CN117720596A - Preparation method for obtaining rebaudioside A and rebaudioside C from stevia mother liquor - Google Patents
Preparation method for obtaining rebaudioside A and rebaudioside C from stevia mother liquor Download PDFInfo
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- CN117720596A CN117720596A CN202211104569.3A CN202211104569A CN117720596A CN 117720596 A CN117720596 A CN 117720596A CN 202211104569 A CN202211104569 A CN 202211104569A CN 117720596 A CN117720596 A CN 117720596A
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- QSRAJVGDWKFOGU-WBXIDTKBSA-N rebaudioside c Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]1(CC[C@H]2[C@@]3(C)[C@@H]([C@](CCC3)(C)C(=O)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)CC3)C(=C)C[C@]23C1 QSRAJVGDWKFOGU-WBXIDTKBSA-N 0.000 title claims abstract description 88
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 title claims abstract description 56
- 239000001512 FEMA 4601 Substances 0.000 title claims abstract description 45
- HELXLJCILKEWJH-SEAGSNCFSA-N Rebaudioside A Natural products O=C(O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)[C@@]1(C)[C@@H]2[C@](C)([C@H]3[C@@]4(CC(=C)[C@@](O[C@H]5[C@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@@H](O[C@H]6[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O6)[C@H](O)[C@@H](CO)O5)(C4)CC3)CC2)CCC1 HELXLJCILKEWJH-SEAGSNCFSA-N 0.000 title claims abstract description 45
- HELXLJCILKEWJH-UHFFFAOYSA-N entered according to Sigma 01432 Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC(C1OC2C(C(O)C(O)C(CO)O2)O)OC(CO)C(O)C1OC1OC(CO)C(O)C(O)C1O HELXLJCILKEWJH-UHFFFAOYSA-N 0.000 title claims abstract description 45
- 235000019203 rebaudioside A Nutrition 0.000 title claims abstract description 45
- 239000001776 FEMA 4720 Substances 0.000 title claims abstract description 44
- 244000228451 Stevia rebaudiana Species 0.000 title claims abstract description 18
- 239000012452 mother liquor Substances 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 239000011347 resin Substances 0.000 claims abstract description 32
- 229920005989 resin Polymers 0.000 claims abstract description 32
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000006092 Stevia rebaudiana Nutrition 0.000 claims abstract description 16
- 239000002244 precipitate Substances 0.000 claims abstract description 16
- 239000000706 filtrate Substances 0.000 claims abstract description 14
- IMNFDUFMRHMDMM-UHFFFAOYSA-N anhydrous n-heptane Natural products CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 239000002120 nanofilm Substances 0.000 claims abstract description 5
- 238000010438 heat treatment Methods 0.000 claims abstract description 3
- 230000001105 regulatory effect Effects 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 239000013078 crystal Substances 0.000 claims description 10
- 241000544066 Stevia Species 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 7
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims description 5
- 238000002425 crystallisation Methods 0.000 claims description 4
- 230000008025 crystallization Effects 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000000967 suction filtration Methods 0.000 claims description 3
- 239000003480 eluent Substances 0.000 claims description 2
- 235000019202 steviosides Nutrition 0.000 abstract description 12
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 abstract description 11
- 229940013618 stevioside Drugs 0.000 abstract description 11
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 abstract description 11
- 239000000047 product Substances 0.000 abstract description 8
- 238000011084 recovery Methods 0.000 abstract description 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 18
- 238000004128 high performance liquid chromatography Methods 0.000 description 8
- 238000000643 oven drying Methods 0.000 description 6
- 239000012528 membrane Substances 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 3
- 229930182470 glycoside Natural products 0.000 description 3
- 150000002338 glycosides Chemical class 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000012046 mixed solvent Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 241000208838 Asteraceae Species 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 235000003368 Ilex paraguariensis Nutrition 0.000 description 1
- 244000188472 Ilex paraguariensis Species 0.000 description 1
- YWPVROCHNBYFTP-UHFFFAOYSA-N Rubusoside Natural products C1CC2C3(C)CCCC(C)(C(=O)OC4C(C(O)C(O)C(CO)O4)O)C3CCC2(C2)CC(=C)C21OC1OC(CO)C(O)C(O)C1O YWPVROCHNBYFTP-UHFFFAOYSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000008123 high-intensity sweetener Substances 0.000 description 1
- 235000021096 natural sweeteners Nutrition 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000013615 non-nutritive sweetener Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- YWPVROCHNBYFTP-OSHKXICASA-N rubusoside Chemical compound O([C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O YWPVROCHNBYFTP-OSHKXICASA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Abstract
The invention discloses a preparation method for obtaining rebaudioside A and rebaudioside C from stevia rebaudiana mother liquor, which comprises the following steps: (1) Regulating pH of the stevia She Mu solution to 5-5.5, heating in water bath, maintaining the temperature for 30-40min, cooling to room temperature, passing through organic molecular film, and collecting film-passing solution; (2) Recovering solvent from the membrane-passing solution, passing the solution through gel resin column, eluting the effluent with gel ion resin column and ethanol, collecting eluate, concentrating, adding low-temperature anhydrous heptane or acetone, stirring, and filtering to obtain filtrate and precipitate; (3) Recrystallizing the filtrate with methanol to obtain rebaudioside A; (4) Recrystallizing the precipitate to obtain rebaudioside C. The preparation method provided by the invention can effectively separate the rebaudioside A and the rebaudioside C from the stevioside mother liquor. The content of rebaudioside A and rebaudioside C in the product is more than 95%, and the recovery rate is more than 90%.
Description
Technical Field
The present invention relates to a method for extracting effective components of stevia rebaudiana, and in particular to a method for preparing rebaudioside A and rebaudioside C from mother liquor of stevia rebaudiana.
Background
Stevia rebaudiana (Stevia rebaudiana Bertoni), also known as stevia rebaudiana (stevia), stevia rebaudiana (Bertoni), etc., is native to yerba mate, brazil, etc., and its leaves contain Stevioside, which is a novel natural sweetener with high sweetness and low calorie, mainly a mixture of several steviosides extracted from the leaves of stevia rebaudiana (Bertoni) of the family Compositae), such as Stevioside (Stevioside), rebaudioside C (RC), rebaudioside A (RA) and Rubusoside. Stevioside belongs to a natural low-calorie high-intensity sweetener, the sweetness of the stevioside is 200-300 times that of sucrose, the calorie is only about 1/250 of that of the sucrose, and the stevioside and Rebaudioside A (RA) which are relatively abundant in content are widely paid attention to a plurality of fields of scientific circles, industries and the like, and the stevioside and the Rebaudioside A (RA) are widely applied to the food processing fields of beverages, foods, seasonings, wines, dairy products and the like. At present, the commercial stevia rebaudiana products mainly adopt STV and RA, the products mainly adopt RC are fewer, and the development of the products mainly adopt RC can meet more social demands.
CN102127130B discloses a method for purifying RC, which comprises preparing 0.5-1% of mother liquor sugar into 0.5-1% of feed liquor, passing through ultrafiltration membrane device, concentrating the concentrated solution at 55-65deg.C, drying the solid liquid to obtain crude sugar, preparing mixed solvent of 85% ethanol, 70% methanol and 85% acetone at a ratio of 3:2:1, and recrystallizing to obtain refined stevioside RC with content of more than 85%. However, in the scheme, the polarities of the components of stevioside, especially RA and RC, are very close, and the content of RA is far greater than the content of RC, so that the proportioning accuracy of the prepared mixed solvent is required to be high, the temperature during crystallization is required to be accurately controlled, the purity of the RC product is reduced too fast or too slow, and the production of the RC product is not facilitated.
Disclosure of Invention
The present invention provides a process for producing rebaudioside A and rebaudioside C from stevia mother liquor.
The technical scheme provided by the invention is as follows: a method for preparing rebaudioside a and rebaudioside C from stevia mother liquor comprising the steps of:
(1) Regulating pH of the stevia She Mu solution to 5-5.5, heating in water bath, maintaining the temperature for 30-40min, cooling to room temperature, passing through organic molecular film, and collecting film-passing solution;
(2) Recovering solvent from the membrane-passing solution, passing the solution through gel resin column, eluting the effluent with gel ion resin column and ethanol, collecting eluate, concentrating, adding low-temperature anhydrous heptane or acetone, stirring, and filtering to obtain filtrate and precipitate;
(3) Recrystallizing the filtrate with methanol to obtain rebaudioside A;
(4) Recrystallizing the precipitate to obtain rebaudioside C.
The stevia rebaudiana She Mu liquid in the step (1) is obtained by dissolving stevia rebaudiana She Mu liquid dry powder in ethyl acetate, wherein the amount of ethyl acetate is 4-6 times of that of the stevia rebaudiana She Mu liquid dry powder.
The normal temperature in the step (1) is 25-30 ℃, and the temperature of water bath heat preservation is 35-45 ℃.
The molecular weight of the organic molecular film in the step (1) is 500-1000.
The gel resin column in the step (2) is prepared from BiophdexG-25 resin or BiophdexG-50 resin. The mass ratio of the gel resin to the membrane-coating liquid is 20-25:1.
The gel ion resin in the step (2) is DEAE Biophdex-25 resin, and the mass ratio of the gel ion resin to the effluent liquid is 15-20:1.
In the step (2), after the effluent liquid enters the gel ion resin, the gel ion resin is washed to be neutral by pure water, and then the gel ion resin is eluted by 50% ethanol which is 3 to 3.5 times of the volume of the gel ion resin.
In the step (2), the eluent is concentrated into concentrated solution, and then anhydrous heptane or acetone with the temperature lower than 0 ℃ is added, wherein the anhydrous heptane or acetone is 0.5-1 times of the volume of the concentrated solution.
In the step (3), the filtrate is concentrated and baked, 3-4 times of methanol is added, and the mixture is crystallized at normal temperature, filtered and baked to obtain the rebaudioside A crystal.
In the step (4), the precipitate is completely dissolved in water (1.5-3 times of the amount of the precipitate), and is placed at 0-5 ℃ for crystallization for 6-10 hours, and then suction filtration and drying are carried out to obtain the rebaudioside C crystal.
The invention has the following advantages:
1. the preparation method provided by the invention can effectively separate the rebaudioside A and the rebaudioside C from the stevioside mother liquor. The content of rebaudioside A and rebaudioside C in the product is more than 95%, and the recovery rate is more than 90%.
2. The preparation method provided by the invention only uses a small amount of solvent in the whole process. In particular, pure water is only used in the whole crystallization process of rebaudioside C, so that the pollution is less, the process is well controlled, and the method is suitable for mass production.
3. The preparation method provided by the invention fully obtains more than 95% of rebaudioside A and rebaudioside C samples from stevia rebaudiana mother liquor sugar. For the product effectively treated with the stevia rebaudiana mother liquor sugar, the production cost of stevia rebaudiana can be indirectly saved.
Drawings
FIG. 1 is an HPLC chromatogram of RA of example one.
FIG. 2 is an HPLC chromatogram of RC of example one.
Detailed Description
The invention will be further illustrated with reference to specific examples.
Example 1
(1) 100g of sweet She Jumu liquid dry powder, 50% of total glycosides, RA 18% and RC15% were dissolved completely with 400ml of ethyl acetate (4 times). Adding 0.1g (1%o) 30% citric acid, adjusting pH to 5.5, maintaining at 40deg.C in water bath for 30min, cooling to room temperature (25deg.C), adding organic molecular membrane with molecular weight of 500, and collecting membrane-passing solution.
(2) The solvent was recovered from the membrane-passing solution, which was passed through 2Kg of gel resin (BiophdexG-25), and the effluent was collected, and then fed into 1.5Kg of gel ion resin (DEAE Biophdex-25), after the completion of the feeding, the solution was washed with pure water to neutrality (pH=7), eluted with 4.5L of 50% ethanol, and the eluate was collected and concentrated to a small amount, i.e., concentrated to 80-90Ml. 100ML of pure anhydrous heptane less than 0 ℃ is added, stirred uniformly and filtered by suction, thus obtaining filtrate and precipitate.
(3) Concentrating the filtrate, oven drying, adding 80ML of methanol, crystallizing at room temperature for 8 hr, vacuum filtering to obtain crystal, oven drying, and detecting sample by HPLC to obtain RA. The RA content in the sample was 96.1%, and the yield was 17.5%. The recovery rate was 93.4%.
(4) The precipitate was completely dissolved in 40ML of water (pH 5 was adjusted with 10% citric acid), left to crystallize at low temperature (2 ℃) for 6 hours, suction filtered to obtain crystals, washed with 5ML of pure water, baked to dryness, and the sample was checked by HPLC and determined as RC. The RC content in the sample was 95.4%, the yield was 14.5%, and the recovery rate was 91.4%.
Example two
(1) 100g of sweet She Jumu liquid dry powder, 50% of total glycosides, RA 18% and RC15% were dissolved completely with 400ml of ethyl acetate (4 times). Adding 0.1g (1%o) 30% citric acid, adjusting pH to 5.5, maintaining at 40deg.C in water bath for 40min, cooling to room temperature (30deg.C), adding organic molecular membrane with molecular weight of 1000, and collecting membrane-passing solution.
(2) The solvent was recovered from the membrane-passing solution, which was passed through 2Kg of gel resin (BiophdexG-50), and the effluent was collected, and then fed into 1.5Kg of gel ion resin (DEAE Biophdex-25), after the completion of the feeding, the solution was washed with pure water to neutrality (pH=7), eluted with 4.5L of 50% ethanol, and the eluate was collected and concentrated to a small amount, i.e., concentrated to 80-90Ml. 100ML of pure anhydrous heptane less than 0 ℃ is added, stirred uniformly and filtered by suction, thus obtaining filtrate and precipitate.
(3) Concentrating the filtrate, oven drying, adding 90ML of methanol, crystallizing at normal temperature for 12 hr, vacuum filtering to obtain crystal, oven drying, and detecting sample by HPLC to obtain RA. The RA content in the sample was 96.5%, the yield was 17.3%, and the recovery rate was 92.7%.
(4) The precipitate was completely dissolved in 45ML of water (pH 5 was adjusted with 10% citric acid), left to crystallize at low temperature (2 ℃) for 10 hours, suction filtered to obtain crystals, washed with 5ML of pure water, baked to dryness, and the sample was checked by HPLC and determined as RC. The RC content in the sample was 95.2%, the yield was 14.3%, and the recovery rate was 90.8%.
Example III
(1) 100g of sweet She Jumu liquid dry powder, 50% of total glycosides, RA 18% and RC15% were dissolved completely with 400ml of ethyl acetate (4 times). Adding 0.1g (1%o) 30% citric acid, adjusting pH to 5.5, maintaining at 35deg.C in water bath for 35min, cooling to room temperature (25deg.C), adding organic molecular membrane with molecular weight of 800, and collecting membrane-passing solution.
(2) The solvent was recovered from the membrane-passing solution, which was passed through 2Kg of gel resin (BiophdexG-50), and the effluent was collected, and then fed into 1.5Kg of gel ion resin (DEAE Biophdex-25), after the completion of the feeding, the solution was washed with pure water to neutrality (pH=7), eluted with 4.5L of 50% ethanol, and the eluate was collected and concentrated to a small amount, i.e., concentrated to 80-90Ml. 100ML of pure anhydrous heptane less than 0 ℃ is added, stirred uniformly and filtered by suction, thus obtaining filtrate and precipitate.
(3) Concentrating the filtrate, oven drying, adding 85ML of methanol, crystallizing at room temperature for 12 hr, vacuum filtering to obtain crystal, oven drying, and detecting sample by HPLC to obtain RA. The RA content in the sample was 96.4%, the yield was 17.4%, and the recovery rate was 93.2%.
(4) The precipitate was completely dissolved in 55ML of water (pH 5 was adjusted with 10% citric acid), left to crystallize at low temperature (2 ℃) for 10 hours, suction filtered to obtain crystals, washed with 5ML of pure water, baked to dryness, and the sample was checked by HPLC and determined as RC. The RC content in the sample was 95.0%, the yield was 14.4%, and the recovery rate was 91.2%.
Claims (10)
1. A method for preparing rebaudioside a and rebaudioside C from stevia mother liquor, comprising the steps of:
(1) Regulating pH of the stevia She Mu solution to 5-5.5, heating in water bath, maintaining the temperature for 30-40min, cooling to room temperature, passing through organic molecular film, and collecting film-passing solution;
(2) Recovering solvent from the membrane-passing solution, passing the solution through gel resin column, eluting the effluent with gel ion resin column and ethanol, collecting eluate, concentrating, adding low-temperature anhydrous heptane or acetone, stirring, and filtering to obtain filtrate and precipitate;
(3) Recrystallizing the filtrate with methanol to obtain rebaudioside A;
(4) Recrystallizing the precipitate to obtain rebaudioside C.
2. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein the stevia rebaudiana She Mu liquid of step (1) is obtained by dissolving stevia She Mu liquid dry powder in ethyl acetate, wherein the ethyl acetate is 4-6 times the amount of stevia She Mu liquid dry powder.
3. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein the normal temperature in the step (1) is 25-30 ℃ and the temperature of the water bath is 35-45 ℃.
4. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein the organic molecular film of step (1) has a molecular weight of 500-1000.
5. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein the gel resin column in the step (2) is formed of biopddexg-25 resin or biopddexg-50 resin, and the mass ratio of the gel resin to the film-passing liquid is 20-25:1.
6. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein the gel ion resin in the step (2) is DEAE biopdex-25 resin, and the mass ratio of the gel ion resin to the effluent is 15-20:1.
7. The process for producing rebaudioside a and rebaudioside C according to claim 1, wherein in the step (2), the effluent is washed with pure water to neutrality and then with 3-3.5 times the volume of 50% ethanol of the resin after the effluent is fed into the gel ion resin.
8. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein in the step (2), anhydrous heptane or acetone is added after concentrating the eluent into a concentrated solution, wherein the anhydrous heptane or acetone is 0.5-1 times the volume of the concentrated solution.
9. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein in the step (3), the filtrate is concentrated, dried, and then 3-4 times methanol is added, and the crystals of rebaudioside a are produced by crystallization at room temperature, suction filtration and drying.
10. The method for producing rebaudioside a and rebaudioside C according to claim 1, wherein in the step (4), the precipitate is completely dissolved in water, and the precipitate is left to crystallize at 0 to 5 ℃ for 6 to 10 hours, and the crystals of rebaudioside C are obtained by suction filtration and drying.
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| CN202211104569.3A CN117720596A (en) | 2022-09-09 | 2022-09-09 | Preparation method for obtaining rebaudioside A and rebaudioside C from stevia mother liquor |
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| CN202211104569.3A CN117720596A (en) | 2022-09-09 | 2022-09-09 | Preparation method for obtaining rebaudioside A and rebaudioside C from stevia mother liquor |
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