CN117701412A - Development of multifunctional natural plant protectant with pepper anthracnose and bacterial disease prevention and control and fertility promoting effects - Google Patents
Development of multifunctional natural plant protectant with pepper anthracnose and bacterial disease prevention and control and fertility promoting effects Download PDFInfo
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- CN117701412A CN117701412A CN202310161219.9A CN202310161219A CN117701412A CN 117701412 A CN117701412 A CN 117701412A CN 202310161219 A CN202310161219 A CN 202310161219A CN 117701412 A CN117701412 A CN 117701412A
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- 238000003892 spreading Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- NJGWOFRZMQRKHT-UHFFFAOYSA-N surfactin Natural products CC(C)CCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-UHFFFAOYSA-N 0.000 description 1
- NJGWOFRZMQRKHT-WGVNQGGSSA-N surfactin C Chemical compound CC(C)CCCCCCCCC[C@@H]1CC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-WGVNQGGSSA-N 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 229960003500 triclosan Drugs 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/25—Paenibacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/12—Bacillus polymyxa ; Paenibacillus polymyxa
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- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
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- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
The invention is a method for effectively solving the problems of difficult control of pepper anthracnose, bacterial wilt, spot disease and the like, not only directly inhibiting the growth of mould and bacterial pathogenic bacteria causing the disease, but also separating and culturing endophytic bacteria with the effects of enhancing the disease immunity and promoting the breeding of host plant pepper from Korean domestic root circle soil to prepare a preparation, and developing and providing a novel natural plant protective agent with novel efficacy of comprehensive plant strengthening (plant strengthener).
Description
[ field of technology ]
The multifunctional natural plant protective agent for preventing and treating pepper anthracnose and bacterial diseases is related to development, and more particularly relates to development of a microbial preparation containing a symbiotic bacterium Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 strain.
The invention relates to a method for controlling crop viruses and plant diseases and insect pests, which is characterized by comprising the steps of carrying out ' industrial technology development for controlling crop viruses and plant diseases and insect pests ' supported by agriculture, forestry and aquatic products food department ' project and ' employment crisis enterprise affiliated research institute R & D special manpower utilization support (R & D) ' business.
[ subject unique number 1545022768, subject name: determination of drug resistance mechanism of Capsici fructus anthracnose and technical development of control agent (2020-2024) ]
[ topic Natural number 1711140886, topic name: microbial mass analysis and action mechanism study (2021-2022) for controlling bacterial porosis (punch disease) of fruit trees ]
[ PREPARATION OF THE INVENTION ]
The yield of capsicum in korean agricultural products is about 1 million 18 million korean yuan (2018), and capsicum is an important blending crop in korea with rice, vegetables, etc. Most of the peppers are planted in the same area, and are planted for several years, and the annual cultivation period is long, so that stable production is greatly lost in the cultivars due to occurrence of pathogenic bacteria of various enzyme diseases, bacteria and viruses. Wherein the loss caused by the anthracnose of the capsicum accounts for about 10% of the total yield value, and reaches about 1000 hundred million Korean yuan.
In severe cases, 50 to 80% of the total amount of the components is lost (see non-patent document 1). Such losses are different depending on the place where anthracnose occurs and the pesticide resistance of pathogenic bacteria, and in korea, domestic anthracnose bacteria were colletotrichum gloeosporioides (Colletotrichum gloeosporioides) in the 90 th century, and recently changed to pepper anthracnose bacteria (Colletotrichum acutatum) (see non-patent document 2). In order to prevent and treat the pepper anthracnose, the pepper variety is provided for farmers to resist the pepper anthracnose, but the limitation in use is somewhat exposed due to the high unit price of seeds, the loss of the pungency of part of varieties, the insufficient compound resistance of pepper viruses and the like. In contrast, the easiest and effective control method is to use chemical bactericides.
The average treatment times of the bactericide used during the pepper planting period are more than 20 times, wherein the average treatment times of the bactericide for preventing and treating anthracnose are 11.2 times (the treatment times of the bactericide are 6-7 times in the proposal of calendar data for preventing and treating anthracnose) (the education data of the loyal pepper synergetic group, 2018). The production of ergosterol (ergosol), which is widely used at present, prevents the production of bactericidal agents and strobilurin-based bactericidal agent-resistant pathogenic bacteria, and the control effect is decreasing (see non-patent documents 3 to 4). Therefore, for effective control of bactericide-resistant anthracnose, there is a need for comprehensive control methods such as development of natural plant protective agents (organisms) that utilize antagonistic or symbiotic microorganisms.
Diseases that cause serious damage to pepper production together with anthrax are bacterial wilt (bacterial wilt), bacterial leaf spot and soft disease (see non-patent document 1). Bacterial wilt is a soil-borne bacterial disease caused by ralstonia solanacearum (Ralstonia solanacearum), mainly occurs at a temperature of 25 ℃ or higher, and does not develop in a low-temperature period. The proper growth temperature of the pathogenic bacteria is 35-37 ℃, and besides the capsicum, the pathogenic bacteria can cause diseases on more than 400 plants such as tomatoes, eggplants, sesame, potatoes and the like. This disease mainly occurs in summer of 7 to 8 months, and once the infected plant dies slowly, the catheter turns brown when the damaged stem is cut off, and bacterial liquid of milk color is secreted in this part. Once an infection is established, the pathogenic bacteria spread along the water, which quickly causes the disease of neighboring plants, and the crop of the whole field dies. At present, there are methods for controlling such diseases by seed-resistant varieties and drip irrigation antibiotics, but there is a strong demand for development of natural plant protection agents using antagonistic microorganisms, which have low control effects against the occurrence of biomass resistance and the like (see non-patent documents 5 to 7).
Bacterial leaf spot of capsicum is caused by (Xanthomonas euvesicatoria), and pathogenic bacteria are transmitted by first infectious source rain, wind or farm tools in spring of the second year after winter of diseased leaves, leaf and stem residues or seeds. First, pathogenic bacteria invade through stomata and wounds of leaves, and initially, lesions start with small grayish brown spots, and if the onset progresses, the whole leaves die to brown (see non-patent document 8). In addition, there is soft rot caused by pectobacterium soft rot (Pectobacter carotovorum), which is a soil-borne disease occurring in Chinese cabbage, sweet pepper, etc., in addition to capsicum. The preparation for preventing and treating the chilli bacterial diseases only comprises copper, an agricultural novel suspending agent, triclosan and the like. The disease of capsicum is caused by various pathogenic bacteria, and the disease is well prevented before the disease occurs because of the huge loss caused by the disease. However, chemical pesticides cannot control mold or bacterial pathogens at the same time, and according to the action mechanism, the preparation mixture of different main components is developed and used together.
The problems of toxicity, environmental pollution and ecological system influence of the chemical pesticides on people and livestock and the sustained occurrence of bactericide resistance of pathogenic bacteria are concerned and developed on environment-friendly natural plant protection agents recently and rapidly increased at home and abroad. In our korea, many pesticides have been used for controlling weeds caused by pests of crops such as capsicum, fruit tree, grain, etc. To solve the various problems caused by this, governments are striving to reduce the amount of fertilizers and pesticides used and drive environmental protection green agricultural cultivation policies. The program was carried out every 5 years from 2001, and the 4 th program (2016-2020) was advanced, so that the planting area ratio of the agricultural products (no agricultural chemical or more) to the environmentally friendly agricultural products was 8% (13 thousands of 3 kilohectares) from 4.5% (7 thousands of 5 kilohectares) during this period, the application was increased by about 78%, and the fertilizer and agricultural chemical usage was reduced by 1.5% or more per year. The material for preventing and controlling diseases and insects, which can replace chemical pesticides, can register environmental-friendly agricultural materials in Korea, and the material is organic agricultural materials and natural plant protective agents. Materials used include a microbial agent and a microbial culture medium which directly utilize the microorganism itself, and a plant material which is extracted. In the aspect of natural plant protection agents, the former is classified into microbial pesticides, and the latter is classified into biochemical pesticides. The microbial basic product analyzes all microbial species (microbiome) in specific natural environments such as soil, plant root circles and sea, selects microorganisms with new functions, sprays the surfaces of leaves after a large amount of culture, or develops a preparation for soil treatment to be used for the purpose of disease and insect control agent (see non-patent document 9).
The microorganism used herein is mainly a natural enemy (antagonistic) microorganism capable of inhibiting plant pathogenic bacteria, a plant growth promoting root canal bacterium (plant growth promoting rhizobacteria, PGPR), an endophytic (endophytic) microorganism that is symbiotic in plant tissues, or the like. Among these microorganisms, many studies on endophytic bacteria of plants have been made in recent decades, and particularly, techniques for treating seeds or seedlings to exert a plant vaccine effect to impart resistance to disease pests, salts and high temperature drying pressure have been developed in korea (see non-patent documents 10 to 12). Endophytic bacteria are not harmful to the host, are symbiotic and beneficial bacteria, and are also present in the space between normal cells or inside the cells. As an action mechanism, nitrogen solidification, solubilization of phosphoric acid, production of iron-binding substances (siderophore), production of plant hormones and antibacterial substances such as lipid peptides (lipopeptides), induction of resistance of disease and pest or promotion of plant growth.
Many endophytic bacteria studied so far are Bacillus, bacillus and paenibacillus
More than 130 kinds of bacteria such as (Paenibacillus), pseudomonas, lysobacter, and Agrobacterium (Agrobacterium), which act on vegetables such as rice, wheat, and other grains, peppers, tomatoes, and potatoes, and inhibit the occurrence of diseases, thereby improving the production of the crops (see non-patent documents 13 to 17).
Bacillus oryzicolaYC7007 is a complex endophyte with rice disease control and growth promotion effects, and shows different action mechanisms according to different host plants. (see non-patent document 18).
The natural plant protectants for controlling plant diseases currently registered in korea are 18 in total (based on 2019), and the main ingredients are Bacillus bacteria and trichoderma mycotica (Tricoderma). Among them, the products actually sold are only 10 Bacillus subtilis (Bacillus subtilis) and 1 Trichoderma harzianum (Trichoderma harzianum) (see non-patent document 9). Among them, 5 are produced by using foreign imported microorganisms, and besides, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Bacillus pumilus (Bacillus pumilus) are used abroad. In order to use the microorganism as a product, the density can be maintained for a long time in a severe environment or circulation, and the prevention and control effect is also good. In this regard, spore forming Bacillus and trichoderma are suitable microorganisms for developing natural plant protection agents, and many researchers have developed biological control agents for pests. It has been found that most bacillus strains exocrine lipopeptide antibacterial substances fengyin, lipopeptide antibiotics (iturin), bacillus metabolic active substances (surfactin) and the like, directly inhibit mold pathogenic bacteria or induce disease resistance of hosts (see non-patent document 19). In addition to the aforementioned Bacillus, recent studies have revealed that the effect of controlling plant diseases is exhibited by Paenibacillus polymyxa (Paenibacillus polymyxa). This strain was originally classified as bacillus (genus) and reclassified from 1993 to a new paenibacillus. Some strains of the bacterial strains not only have the inhibition effect of plant pathogenic mould, but also can effectively inhibit bacterial disease bacterial wilt. It has been confirmed that growth of various crops is promoted by nitrogen fixation, phosphate dissolution, secretion of plant growth hormone, and the like. In particular, fusarium (fusarium), bacteriocin (bacteriocin), lipopeptides, and the like, and polymyxin (polymyxin) has been utilized as a pharmaceutical product for inhibiting pathogenic bacteria in humans long ago. (see non-patent documents 20 to 23).
At present, several products of bacillus at home and abroad are commercialized and used for controlling plant diseases, but the control effect is relatively lower than that of chemical pesticides, and the diffusion in the market is slow. In order to develop a more effective product, technically, it is necessary to select and extract excellent microorganisms capable of effectively inhibiting pathogenic bacteria of a subject, and at the same time, it is necessary to consider a development strategy of an action mechanism.
[ non-patent literature ]
Non-patent document 1, high Yeongjin, gold Sangsoo, poSeoki, poJongdae (2004).
And (5) ecologically and preventing and controlling diseases and pests of the vegetables. Technology center for flavoring vegetables at university of Shuntian agricultural life sciences college
Non-patent document 2 Kim, j.t., par, s.y., choi, w.b., lee, y.h., kim, h.t. (2008).
Characterization of Colletotrichum isolates causing anthracnose of pepper in Korea.
Plant Pathol.J.24:17-23.
Non-patent document 3, greenhouse density investigation of pepper anthracnose Colletotrichum acutatum, counter selection medium establishment and utilization. Plant disease study 11:21-27.
Non-patent documents 4, jin Xinhua, min Zhiying and Jin Xingtai (2019).
Resistance performance and mechanism of Strobilurin-based bactericide against Pyraclostrobin pepper anthracnose Colletotrichum acutatum. And the paper for the pesticide science is 23:202-211.
Non-patent documents 5, li Hengzhu, zhao Enjing, jin Naxi, color, and Li Shanyu (2011).
The onset phytosis at high temperature and tomato disease response of Ralstonia solanacearum isolated in korea was studied 17:326-333.
Non-patent documents 6: li Yingji, jiang Xian (2013). Physiological, biochemical and genetic properties of Ralstonia solanacearum system isolated from capsicum. Plant disease study 19:265-272.
Non-patent documents 7, li Xiumin, guo Yanxiu, li Jingxi and Jin Xingtai (2015). The bactericide has the control effect on pepper wilt. And the paper for the pesticide science is 19:323-328.
Non-patent document 8, kyeon, m.s., son, s.h., noh, y.h., kim, y.e.,
Lee,H.I.,Cha,J.S.(2016).Xanthomonas euvesicatoria causes bacterial spot disease on pepper plant in Korea.Plant Pathol.J.32:431-440.
non-patent document 9 discloses medals, jin Taixun and Qiu Chengmin (2020).
Parent environment agricultural materials industry current situation and policy subject, korean rural economic institute
Non-patent document 10 Hossain, M.T, khan, A., chung, E.J., harun, R.M.,
Chung,Y.R.(2016).Biological control of rice bakane by an endophytic Bacillus oryzicolaYC7007.Plant Pathol.J.32:228-241.
non-patent document 11: harun, M.R., kim, H.J., yeom, S.I., yu, H.A., moon, S.S., kang, Y.
J.,Chung,Y.R.(2018).Bacillus velezensisYC7010 enhances plant defenses against brown planthopper through transcriptomic and metabolic changes in rice.
Front.Plant Sci.9:1904.doi:10.3389/fpls.2018.01904.
Non-patent document 12, baek, D., rokibuzzaman, m., khan, a, kim, m.c., par, h.j., yun, D.
J.,Chung,Y.R.(2020).Plant-growth promoting Bacillus oryzicola YC7007
modulates stress-response gene expression and provides protection from salt
stress.Front.Plant Sci.,9Jan.doi.org/10.3389/fpls.2019.01646.
Non-patent document 13 Costa, J.M. & Loper, J.E. (1994) Characterization of siderophore)
production by the biological control agentEnterobacter cloacae.Mol.Plant-MicrobeInteract.7:440-448.
Non-patent document 14 Timmusk, S., wagner, E.G.H. (1999). The plant-growth promotingrhizobacterium Paenibacillus polymyxainduces changes in Arabidopsis
thalianagene expression:a possible connection between biotic and abiotic stress
responses.Mol.Plant-Microbe Interact.12:951-959.
Non-patent document 15 Wakelin, S.A., warren, R.A., harvey, P.R.R.Ryder, M.H. (2004).
Phosphate solubilization byPenicilliumspp.closely associated with wheat roots.Biol.
&Fertil.Soils 40:36-43.
Non-patent document 16, rosenblueth, m., & Martinez-romio, e. (2006). Bacterial strain
endophytes and their interactions with hosts.Mol.Plant-Microbe Interact.19:827-837.
Non-patent document 17, bi, f., yasir, m., song, g.c., lee, s.y., and Chung, y.r. (2012).
Diversity and characterization of endophytic bacteria associated with tidal flat plantsand their antagonistic effects on oomycetous plant pathogens.Plant Pathol.J.28:20-31
Non-patent document 18: chung, E.J., hossain, M.T, khan, A, kim, K.H., jeon, C.O., chung, Y.R. (2015) Bacillus oryzicolasp.nov, an endophytic bacterium isolated from the
roots of rice with antimicrobial,plant growth promoting and systemic resistance
inducing activities in rice.Plant Pathol.J.31:152-164.
Non-patent document 19 Islam, M.T., rahman, M.M., pandey, P., boehme, M.M., haesaert, G. (2020) Bacilli and Agrobiotechnology: phytostimulation and Biocontrol (Vol 2) Springer.335p.
Non-patent document 20 Chung, Y.R., kim, C.H., hwang, I., chun, J. (2000).
Paenibacillus koreensissp.nov.,a new species that produces an iturin-like antifungal compound.Inter.J.Syst.&Evol.Microb.50:1495-1500.
Non-patent document 21, jeong, h.y., choi, s.k., ryu, c.m., park, s.h. (2019).
Chronicle of a soil bacterium:Paenibacillus polymyxaE681 as a tiny guardian of plant and human health.Front Microbiol.15March 2019doi.org/10.3389/fmicb.2019.00467
Non-patent document 22, yi, j., zhang, d., cheng, y., tan, j., luo, y. (2019).
The impact of Paenibacillus polymyxaHY96-2 luxS on biofilm formation and control of bacterial wilt.App.Microb.&Cell Physiol.103:9643-9657.
Non-patent document 23, grady, e.n., macDonald, j., liu, l., richman, a., yuan, z. (2016).
Current knowledge and perspectives ofPaenibacillus:a review.Microb.Cell Fact.15:203-211
Non-patent document 24: yoon, s.h.h., ha, s.m., lim, j.m., kwon, S.J @ and & gt
Chun,J.(2017).A large-scale evaluation of algorithms to calculate average nucleotide identity.Anton van Leeuwen.110:1281-1286.
[ invention ]
[ problem ] to be solved
Here, the control of anthracnose, bacterial wilt and spot disease of capsicum is difficult, and the present invention is to solve the problem of the disease, not only directly inhibit the growth of mold and bacterial pathogenic bacteria causing the disease, but also promote the increase and propagation of disease immunity of capsicum as a host plant, and separate and culture endophytic bacteria having all effects from korean domestic root-circle soil, and prepare a preparation to develop a novel natural plant protectant with comprehensive plant strengthening (plant strengthener) efficacy.
[ means for solving the problems ]
In order to solve the above problems, the present invention provides a novel microbial Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP) strain.
Furthermore, the characteristics of the Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP) strain provided in the above description include the base sequence 16S rRNA genetic gene, the same base sequence is described by the number of SEQ ID NO. 1,
the Bacillus polymyxa (Paenibacillus polymyxa) JG90 (KCTC 14388 BP) strain has antagonistic ability to more than 1 pathogenic bacteria selected from the group consisting of pepper anthracnose, pepper bacterial wilt and apple anthracnose
In addition, the Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP) strain provided in the above description is characterized in that the pathogenic bacterium of pepper anthracnose is Bacillus aculeatus (Colletotrichum acutatum), and the pathogenic bacterium of eggplant wilt is Rhizoctonia solani (Ralstonia Solanacearum)
In addition, the Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (KCTC 14388 BP) strain provided in the above description has antagonistic capability on more than 1 pathogenic bacteria selected from the group consisting of fruit tree fusarium wilt, peach perforated pathogen, pepper soft rot and pepper spot pathogen.
In addition, the Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP) strain provided in the above is characterized in that the pathogenic bacterium of soft rot of capsicum is pectobacterium (Pectobacterium carotovorum), and the pathogenic bacterium of Rhizoctonia cerealis is Xanthomonas vesiculosus (Xanthomonas euvesicatoria)
In addition, the Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (KCTC 14388 BP) strain provided in the above description is characterized by having the effects of promoting fertility of capsicum and inducing immunity.
The present invention also provides a microbial preparation containing the strain or an active ingredient of a culture medium thereof.
In addition, the microbial preparation is characterized by being used for fertilizer application or natural plant protection agent application.
[ Effect of the invention ]
According to the invention, the Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 is taken as a domestic indigenous bacterium in Korea, can inhibit important plant diseases anthracnose of capsicum and bacterial wilt, soft disease and spot disease under the induction of antibiotic secretion and plant immunity, is a multifunctional bacterium with the growth inhibition capability of important bacterial pathogenic bacteria of fruit trees, and can provide excellent microorganisms capable of playing roles of natural plant protectants and microbial fertilizers
In addition, although spreading nationwide at present, it has excellent antibacterial activity against fruit tree black rot (burn) and ulcer pathogenic bacteria which are difficult to control, and at the same time, it is possible to provide novel multifunctional microorganisms having disease immunity inducing and breeding promoting effects of host plants and microbial preparations using the same.
[ brief description of drawings ]
FIG. 1 is a photograph showing the result of the test for inhibiting pathogenic bacteria of pepper anthracnose in example 1 of the present invention. FIG. 1A shows the results of a counter culture test for separating microorganisms and pathogenic bacteria, and B shows the results of a test for inhibitory effect on a strain having good inhibitory activity.
FIG. 2 is a diagram showing the nucleotide sequence of 16S rRNA gene of JG90 strain
FIG. 3 is a drawing showing the number of systems obtained by 16S rRNA gene sequence analysis of the JG90 strain.
FIG. 4 is a photograph showing a result of comparative analysis of a standard strain by gene analysis (BOX-PCR) of the JG90 strain.
FIG. 5 is a photograph showing the result of comparison of the treatment areas of the control effect of the JG90 strain on pepper anthracnose.
FIG. 6 is a comparison result of treatment areas illustrating the apple anthracnose control effect of the JG90 strain
FIG. 7 is a comparison result of treatment areas for illustrating the control effect of the JG90 strain on pepper blight
FIG. 8 is a photograph showing the inhibitory effect of the JG90 strain on the main plant pathogenic bacteria.
FIG. 9 is a photograph showing the results of comparison of treated areas of the control effect of the bacterial perforin disease of peach of the JG90 strain.
FIG. 10 is a photograph showing the result of comparing the treated areas of the immunity induction effect of the JG90 strain on pepper anthracnose.
FIG. 11 is a photograph showing the effect of increasing the number of fruits in the growth of the JG90 strain.
In fig. 11, (a) shows the effect after 4 weeks and (b) shows the effect after 8 weeks.
FIG. 12 is a graph showing the effect of JG90 strain on promoting pepper growth.
FIG. 13 is a graph showing growth according to the culture medium and the culture period of the JG90 strain.
[ for embodying the invention ]
The present invention will be described in detail below with reference to examples. The terms or words used in the specification and the scope of the request are not to be construed as limited to general or dictionary meanings. The inventor can define the term concept under the reasonable definition principle in order to describe his own invention by the best method, and should explain the concept and meaning by conforming to the meaning according to the technical idea of the invention. Therefore, the examples described in the detailed description table are only the most preferable examples of the present invention, and do not represent the technical idea of the present invention, and therefore, it is understood that the present application time may replace the existence of various equivalents and modified examples thereof.
The inventor discovers a novel multifunctional microorganism with the disease immunity induction and growth promotion effects of host plants, and the microorganism can inhibit the occurrence of important plant diseases such as anthracnose, bacterial wilt, soft disease and zebra spot of capsicum due to the secretion of antibiotics and the induction of plant immunity, and has the growth inhibition capability of important bacterial pathogenic bacteria of fruit trees.
Thus, the present invention discloses a novel microorganism Paenibacillus polymyxa (Paenibacillus polymyxa
polymyxa) JG90 (accession number KCTC14388 BP).
The present invention will be described in detail below using examples of embodiments.
EXAMPLE 1 isolation and identification of the JG46 Strain
(1) JG90 Strain isolation
The JG9 strain was isolated from the root internal tissue of grass (Miscanthus sinensis) in industrial soil, and it was grown on the first green of the chinese cognac (strain) in jin. Firstly, in order to isolate endophytic bacteria, root samples are washed with tap water, cut into small pieces, and soaked in a 1% NaOCl solution for 10 minutes to perform surface sterilization. After these chips were washed with sterilized water, 1/10 of the sterilized chips were placed on TSA medium (Tryptic Soy Broth g, agar 16 g/distilled water 1 liter) and cultured for about 2 to 3 days to confirm whether or not surface sterilization was normal, and whether or not bacteria were grown on the surface was observed. 1g of root fragments in which bacteria hardly grow was taken, 9 ml of distilled water sterilized in an autoclave was added, and after grinding with a sterilized mortar and mortar, 1 ml of distilled water was added thereto, and the solution was diluted 10 times (10-3, 10-4, 10-5) in this order. After 0.1 ml of the diluted solution was poured onto 1/10TSA medium and spread evenly, the solution was cultured in a medium at 28℃for 1 to 2 weeks.
The grown bacteria 350 were isolated in a pure form, and 15 strains having a good inhibitory effect were selected as shown in the following Table 1 (see FIG. 1) as a result of the cultivation of the bacteria against the pathogenic bacteria of pepper anthracnose
Among the selected strains, the 1 most suitable for the production of the growth rate (JG No. 90) was finally selected, and this was designated as JG90 strain.
In Table 1 below, the antagonistic ability of the bacteria was measured for the extent of inhibition of hypha growth (+3 mm, ++,4-5mm ++,6-7 mm) after culturing at 28℃for 4-6 days, R was the fungicide resistant strain, and S was the fungicide sensitive strain.
[ Table 1]
(2) Identification of JG90 Strain
To identify the above isolated JG90 strain, the nucleotide sequence of the 16S rRNA gene was determined (see 1499bp, FIG. 2), and the phylogenetic position was studied by performing an interoperability search with the database of GenBank/EMBL/DDBJ.
FIG. 3 shows the number of systems produced by 16S rRNA gene sequence analysis of the JG90 strain. The number of intersections in fig. 3 shows the bootstrap value (boottrap value) coming out in 1000 iterations.
On the other hand, the average nucleic acid identity (ANI, average nucleotide identity) of the base sequences of the genetic genomes was compared with the following standard-like strain (type patterns), and the results are shown in Table 2 (see non-patent document 24)
[ Table 2 ]
| Hit taxon | ANI(％) | ANI coverage(％) |
| Paenibacillus polymyxa | 97.16 | 98 |
| Paenibacillus polymyxa ATCC 842(T) | 92.74 | 84 |
| Paenibacillus jamilae | 92.49 | 83.2 |
| Paenibacillus polymyxa E681 | 89.52 | 81.1 |
| Paenibacillus seodonensis DCT19(T) | 87.50 | 1.9 |
| Paenibacillus brasilensis PB172(T) | 87.39 | 65.9 |
| Paenibacillus kribbensis AM49(T) | 87.20 | 64.4 |
| Paenibacillus peoriae DSM 8320(T) | 86.78 | 62.1 |
| Paenibacillus maysiensis SX-49(T) | 86.49 | 59.1 |
| Paenibacillus barcinonensis BP-23(T) | 86.09 | 1.4 |
| Paenibacillus qingshengii S1-9(T) | 85.72 | 1 |
| Paenibacillus panacisoli Gsoil 1411T | 85.20 | 2.3 |
| Paenibacillus polysaccharolyticus | 85.19 | 1.1 |
| Paenibacillus illinoisensis NRRL | 84.95 | 1.5 |
| Paenibacillus massiliensis Smarlab | 84.68 | 2.2 |
Referring to Table 2, the isolated JG90 strain was confirmed to be Paenibacillus polymyxa (Paenibacillus polymyxa) by average nucleic acid identity analysis in more accurate genetic profile as a result of 16s rRNA similarity analysis
FIG. 4 shows the results of a standard strain-to-gene comparative analysis using gene analysis (BOX-PCR) of the JG90 strain. The polymerase (polymerase) used for gene analysis (BOX-PCR) was Platinum Taq DNA polymerase High Fidelity (Invitrogen), and BOXAR1 (5' -CATCGGCAAGGCGACGCTGACG-3) was used as primer. The initial denaturation (initial denaturation) of PCR conditions was carried out at 95℃for 7 minutes, denaturation, annealing (annealing) and elongation (elongation) of 35 repeated processes at 90℃for 30 seconds, 40℃for 1 minute, 72℃for 3 minutes, and finally final elongation at 72℃for 10 minutes, respectively, was increased.
For the PCR product obtained through this procedure, whether or not it was increased was confirmed by 1% LE agaroose gel (Seake). FIG. 4 shows that M is a 1kb marker,1 is a JG22 strain, 2 is a JG90 strain, 3 is a JG91 strain, 4 is a JG93 strain, 5 is (Paenibacillus jamiliae) KCTC13919, 6 is (Paenibacillus polymyxa) KACC10098, and 7 is (Paenibacillus polymyxa) E681 standard strain
As shown in FIG. 4, in order to perform this comparison in more detail with a standard strain having the same or similar academic name as the JG90 strain, a gene analysis (BOX-PCR) was performed, and as a result, the JG90 strain was identified as being different from the standard strain.
Thus, the microorganism of the present invention is designated as Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 strain, and is hosted by a hosting facility, and the entrusted number KCTC14388BP is given from the biological resource center (KCTC: korean Collection for Type Culture) of the Korean institute of life and electronics, 11.month and 24.2020.
(3) Whole genome (whole genome) analysis of JG90 Strain
To analyze the whole Genome of the above isolated JG90 strain, the whole gene was identified and analyzed using the Trueback ID-Genome system of chun lab and Ex Bio clone, the results of which are shown in table 3 below.
[ Table 3 ]
| Strain name | JG90 |
| Genome·size·(bp) | 5,630,584 |
| No.·of·contigs | 3 |
| DNA·G+C·content(％) | 45.33 |
| No.·of·CDSs | 5.186 |
| No_·of·rRNA·genes | 39 |
| No.·of·tRNA·genes | 111 |
| Mean·of·CDS·lengths·(bp) | 923.59 |
| Median·of·CDS·lengths·(bp) | 795 |
| Mean·of·intergenic·engths·(bp) | 165.77 |
| Nedian·of·intergenic·engths(bp) | 121 |
Example 2: identification of pepper anthracnose prevention and control effect of JG90 strain preparation
To identify the effect of the JG90 strain on controlling pepper anthracnose, the effect of controlling disease was investigated in two places in different areas as shown in table 4 of the powder formulation sample. The test of prevention and treatment efficacy is carried out according to the disorder block method, 3 times of repeated investigation of JG90 microbial agents are carried out, the microbial agents are diluted 1000 times and 2000 times, and 4 times of leaf treatment are carried out every 10 days in the initial stage of disease. The number of incidences of 200 fruits or more per treated area after 10 days of treatment with the final microbial agent was investigated and the incidences and the ratio (%) were shown. The phytotoxicity investigation was performed on days 7, 14 and 21 after the treatment of the JG90 microbial agent, and no phytotoxicity was observed (see FIG. 5).
The results of investigation of the effect of controlling pepper anthracnose with respect to the JG90 microbial agent are shown in table 4 below. From these results, it was confirmed that the present microbial agent can be used for the control of pepper anthracnose.
[ Table 4 ]
Example 3 identification of apple anthracnose control Effect of JG90 Strain preparation
To identify the apple anthracnose control effect of the JG90 strain, the disease control effect was investigated on an apple farm as shown in table 5 with the powder formulation samples. The test of control efficacy was carried out according to a completely arbitrary arrangement method, the pretreatment-free and repeated investigation of the JG90 microbial agent was carried out 3 times, the microbial agent was diluted 1000 times and 2000 times, and the foliar spray treatment was carried out 7 times every 10 days in the initial stage of the onset of the disease. Control values after 10 days of final microbiologic treatment, the number of ill fruits per treated area was investigated and the results showed a ill rate (%). The phytotoxicity investigation was performed 3, 5 and 7 days after the treatment of JG90 microbial agent, and no phytotoxicity was observed (see FIG. 6)
Table 5 below shows the results of investigation of the effect of controlling apple anthracnose of JG90 microbial agents. From the results, the microbial agent can be used for preventing and controlling anthracnose of apples
[ Table 5 ]
EXAMPLE 4 identification of the control Effect of bacterial wilt of Capsici fructus of JG90 Strain
In order to examine the effect of JG90 strain on controlling bacterial wilt of capsicum, the powder formulation samples were investigated for the effect of controlling disease in two different areas of the test land. The control efficacy test was conducted by repeating the investigation 3 times in the treatment area of the microorganism agent JG90 without treatment according to the disorder block method, and the microorganism agent was diluted 1000-fold and 2000-fold, and the perfusion treatment was conducted 4 times every 7 days in the initial stage of the onset of the disease. The control value was examined for the number of the entire fruit tree plants per treated area after 7 days of treatment with the final microbial agent, and the result showed the disease occurrence rate (%). The phytotoxicity investigation was performed on days 7, 14 and 21 after the treatment with the JG90 microbial agent, and no phytotoxicity was observed (see fig. 7).
Table 6 below shows the results of investigation of the effect of controlling bacterial wilt of capsicum against JG90 microbial agents. From these results, it can be seen that the microbial agent can be used for preventing and treating pepper wilt
[ Table 6 ]
EXAMPLE 5 inhibition of the primary plant pathogenic bacteria of the JG90 Strain
In order to examine the inhibition effects of the important plant pathogenic bacteria such as zebra bacteria, soft bacteria, fruit tree black rot bacteria and peach perforation bacteria of the capsicum and fruit tree of the JG90 strain, the growth inhibition effects of the bacteria are investigated as follows
The JG90 strain was cultured in a 1/10TSB liquid medium at 28℃for 72 hours with shaking (180 rpm), and the extent of inhibition of the culture broth was examined by the agar well diffusion method (agar well diffusion method). After a hole having a diameter of 10 mm was punched by laying pathogenic bacteria on the medium, 100. Mu.l of the culture solution of the JG90 strain was placed in the hole, and the extent of inhibition of growth of pathogenic bacteria formed around the hole was confirmed, and the result thereof is shown in FIG. 8. In FIG. 8, (a) shows an antibacterial effect against fruit tree fusarium wilt, (b) against pepper soft rot and (c) against peach bacterial perforin.
Referring to FIG. 8, it can be seen that the JG90 culture solution has a good inhibitory effect on not only anthracnose of capsicum, but also pathogenic bacteria.
EXAMPLE 6 identification of control Effect of bacterial Confucius of JG90 Strain preparation on peach bacterial perforin
In order to examine the effect of preventing and controlling the bacterial perforin disease of peach bacteria of JG90 strain, the disease preventing and controlling effect of the powder formulation sample was investigated. The test of control efficacy was carried out according to a completely arbitrary configuration method, the pretreatment-free and repeated investigation of the JG90 microbial agent was carried out 3 times, the microbial agent was diluted 1000-fold and 2000-fold, and the foliar treatment was carried out 3 times every 10 days before the onset of disease. The control value was examined for the number of diseased leaves of 200 leaves or more per treated area after 11 days of treatment with the final microbial agent, and the result showed a diseased leaf rate (%). The phytotoxicity investigation was performed on the appearance of the microbial agent at 3, 5 and 7 days after the treatment with the JG90 microbial agent, but no phytotoxicity was observed (see FIG. 9).
The test results of the control effect on the bacterial pore disease of peach of the JG90 microbial agent are shown in Table 7 below. From these junctions
As can be seen from the results, the microbial agent can be used for preventing and treating bacterial perforin disease of peach
[ Table 7 ]
EXAMPLE 7 identification of Capsici immunity-inducing efficacy of JG90 Strain
In order to identify the pepper immunity induction effect of the JG90 strain, the control effect was investigated as shown below
After germination of pepper seeds (color), sowing in a ceramic pot (diameter 10 cm) filled with garden bed soil, from a plant growing bed (28-30 ℃, relative humidity above 80%, darkness for 16 hours, light for 8 hours) to 3-4 leaf period, and after seedling cultivation, 10ml of JG90 strain culture solution (0.5 TSB,28 ℃,48 hours, 160rpm, O.D not less than 1) on the periphery of the root circle of the pepper in the ceramic pot is treated. After 3 days of strain treatment, mycelium disks of pepper anthracnose Colletotrichum coccodes, which were cultured in oat agar (oatm al agar) medium for about 3 weeks, were placed on pepper seedlings. 1 germ plate is placed on 2 leaves of each plant, and the inoculation is repeated for 5 times, and 10 leaves are inoculated. The treated ports were inoculated with germs covered with plastic cloth, and the extent of plaque necrosis occurring after 6 days of the birth bed was investigated. The incidence is divided into 0 to 3 (0: no disease symptoms, 1: about 10% of the lesions around the blades around the mycelium disks, 2: about 30% to 50% of the lesions around the blades around the mycelium disks, 3: more than 50% of the blades around the mycelium disks are necrotic), and the incidence is calculated from the incidence obtained from the disease investigation according to the following mathematical formula 1.
[ mathematics 1]
Incidence (%) = { [ (number of diseased leaves×1) + (number of diseased leaves×2) + (number of diseased leaves×3) ]/number of diseased leaves×3} ×100 were examined
The results of investigation of the effect of the soil root loop treatment of the EG90 strain on the control of pepper anthracnose are shown in Table 8 below, and comparative photographs of the treatment areas illustrating the immune induction effect of pepper anthracnose are shown in FIG. 10
[ Table 8 ]
Referring to table 8 and fig. 10, the jg90 strain suspension was inoculated with germs after 3 days of treatment in soil, and the results of investigation on the control effect showed that the incidence of leaves treated with only pathogenic germs was 2.4±0.8, the treatment area of jg90 strain was 0.9±0.7, and that the control price of 62.5% was confirmed to be excellent in the immune induction effect of host anthracnose.
EXAMPLE 8 identification of efficacy of JG90 Strain to promote Capsici fructus propagation
To identify the efficacy of promoting pepper growth by JG90 strain treatment, the efficacy of plant growth was investigated as follows.
After germination of pepper seeds (color), sowing in a ceramic basin Pot (diameter 10 cm) placed in gardening bed soil, spraying 10ml of JG90 strain culture solution (0.5 TSB,28 ℃,48h,160rpm, O.D not less than 1) on pepper root circle from a plant growth bed (28-30 ℃ C., relative humidity above 80%, darkness for 16 hours, light for 8 hours) to 3-4 leaf period. After two weeks of strain treatment, the growth degree and the pure weight of the stems and the roots are respectively investigated after the strain is planted in a plant fertility bed. The control zone was treated with an equivalent amount of medium (0.5 TSB). Each treatment was repeated 3 times, 5 plants per time. In the following table 9, the results of efficacy investigation of the JG90 strain for promoting pepper growth are shown.
[ Table 9 ]
Referring to Table 9, after two weeks of treatment of the JG90 strain suspension in soil, the pure weight and root and stem length of the capsicum were investigated, and the results showed that the pure weight increased by 12.0 grams over 11.4 grams of the untreated zone, without significant differences, with the JG90 strain treatment. But the root length was longer than 14.0 cm without treatment, 18.0 cm and longer, but the stem length was not different. As described above, the seedlings grown on the breeding bed were transplanted again to a Datao pot (diameter: 18 cm), and the seedlings were cultivated in the open field for 2 months, and the breeding state was examined, and the results are also shown in fig. 11 and 12.
Referring to FIGS. 11 and 12, it can be seen that the pepper treated with the JG90 strain has significantly improved plant height and the like as compared to the untreated region. From this result, the effect of promoting the propagation of roots and stems of capsicum was confirmed by the treatment of JG90 strain at seedling stage, and it was expected that the yield of capsicum after growth could be increased.
EXAMPLE 9 JG90 growth comparison according to Medium and culture time of the Strain
In order to prepare the JG90 strain, absorbance (600 nm) was measured in 5 media (GM, SYM, NB, TSB and 0.5 TSB) at 12, 24, 36, 48, 60 and 72 hours, respectively, to find a large amount of culture and appropriate growth conditions, and the results are shown in FIG. 13.
Referring to FIG. 13, the JG90 strain at 48 hours of cultivation showed the highest absorbance in the 3 SYM, TSB and 0.5TSB media, confirming suitability for cultivation. The medium used was the Difco medium which is being marketed, the main ingredients being as follows: GM; soypepton 2g,Yeast Extract 1g,Sucrose 0.5g,MgSO44g,MgCl22g,NaCl 10g,SYM:Yeast Extract 5g,Sucrose 10g,NB:Beef Extract 3g,Peptone 5g,TSB:Pancreatic digest of Casein 17g,Papaic digest of soybean 3g,Dextrose 2.5g,Sodium Chloride 5g,Dipotassium Phosphate 2.5g,0.5TSB:Pancreatic digest of Casein 8.5g,Papaic digest of Soybean 1.5g,Dextrose 1.25g,Sodium Chloride 5g,Dipotassium Phosphate 1.25g.
EXAMPLE 10 preparation procedure of JG90 Strain
For the production of the JG90 strain, powder and liquid forms were prepared as described below in order to maintain the density during the activity and storage.
| Differentiation of | Pure weight (g) | Root length (cm) | Length of stem (cm) |
| No treatment | 11.4a | 14.0a | 26.0a |
| JG90 microbial agent | 12.0a | 18.0b | 26.5a |
Culturing the strain in a large-scale fermentation machine (more than 1 ton), mixing the obtained bacterial culture solution or thallus obtained after centrifugal separation with clay mineral such as geolite, kaolin, bentonite, peat at a weight ratio of 1:100, air drying at low temperature, and pulverizing to obtain powder paste. Simultaneously, the bacterial culture and the clay mineral are mixed in a weight ratio of 1:100 to prepare a liquid suspension
As a result of examining the density of the above-mentioned preparation samples, it was revealed that most of the preparations contained 10 9 The liquid suspension of cfu/g or more and the clay mineral mixed preparation also contains 10 9 Density above cfu/ml.
The practical examples of the present invention described above are disclosed for solving the technical problems, and if a person having ordinary knowledge in the technical field of the present invention can make various modifications, alterations, additions, etc. within the spirit and scope of the present invention, such modifications, alterations, etc. are within the scope of the following claims.
[ mechanism under support ]
Hosting organization name, korean institute of life and engineering
Trusted number KCTC14388BP
Trusted date 20201124
Claims (9)
1. A novel microorganism, paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number KCTC14388 BP).
2. In the item 1, in which the first and second substrates,
the strain includes a Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP) strain characterized by the 16SrRNA gene of the base sequence described in SEQ ID NO. 1.
3. In item 1, the strain is Paenibacillus polymyxa (Paenibacillus) having antagonism against at least 1 pathogen selected from the group consisting of pepper anthracnose, pepper fusarium and apple anthracnose
polymyxa) JG90 (accession number KCTC14388 BP).
4. In the third item, the pathogenic bacteria of the pepper anthracnose and the pepper blight are respectively Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP) strains characterized by Colletotrichum acutatum and Ralstonia solanacearum.
5. In item 1, the strain is Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (trusted plaited) having antagonism against at least 1 pathogen selected from the group consisting of fruit tree black rot, peach pit rot, pepper soft rot, pepper spot
No. KCTC14388 BP).
6. In a fifth aspect, the pathogenic bacteria of the soft rot pathogen of capsicum and the spot pathogen of capsicum are respectively
Pectobacterium Carotovorum and Xanthomonas Euvesicatoria A strain of Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP).
7. In item 1, the strain is a Paenibacillus polymyxa (Paenibacillus polymyxa) JG90 (accession number: KCTC14388 BP) strain characterized by promoting plant growth and inducing immune efficacy of capsicum.
8. A microbial preparation comprising the strain of any one of items 1 to 7 or an active ingredient of a culture solution thereof.
9. In item 8
The microbial preparation is characterized by being a microbial preparation for fertilizer or a natural plant protective agent.
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