CN117683635A - Incubator with detect function - Google Patents
Incubator with detect function Download PDFInfo
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- CN117683635A CN117683635A CN202410152967.5A CN202410152967A CN117683635A CN 117683635 A CN117683635 A CN 117683635A CN 202410152967 A CN202410152967 A CN 202410152967A CN 117683635 A CN117683635 A CN 117683635A
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- 239000012535 impurity Substances 0.000 claims abstract description 54
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 238000001514 detection method Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000012528 membrane Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 82
- 238000004140 cleaning Methods 0.000 claims description 24
- 230000001012 protector Effects 0.000 claims description 18
- 238000005070 sampling Methods 0.000 claims description 18
- 238000005452 bending Methods 0.000 claims description 13
- 238000010992 reflux Methods 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 6
- 238000005202 decontamination Methods 0.000 claims description 2
- 230000003588 decontaminative effect Effects 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims description 2
- 230000001502 supplementing effect Effects 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 9
- 230000010261 cell growth Effects 0.000 abstract description 7
- 208000002720 Malnutrition Diseases 0.000 abstract description 5
- 230000001071 malnutrition Effects 0.000 abstract description 5
- 235000000824 malnutrition Nutrition 0.000 abstract description 5
- 208000015380 nutritional deficiency disease Diseases 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 238000004113 cell culture Methods 0.000 abstract description 4
- 210000003608 fece Anatomy 0.000 abstract description 4
- 238000001125 extrusion Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
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Abstract
The invention relates to the field of cell culture equipment, in particular to an incubator with a detection function. The technical problems are as follows: the existing device can only observe the growth condition of cells after the culture bag is taken out by opening the culture box, but when the culture bag is taken out from the culture box to be in contact with the outside, the observation result is inaccurate, the representativeness of the observation result is reduced, meanwhile, in the process of cell growth, the cells can produce impurities such as excrement, when the impurities are too much, the absorption of the cells to the culture solution is easily affected, and the conditions such as malnutrition and the like of the cells are caused. The technical implementation scheme of the invention is as follows: an incubator with a detection function comprises an incubator, a mounting rack and the like; the inner side of the incubator is provided with a mounting rack. The invention filters the cell liquid through the filter membrane, intercepts impurities such as excreta of cells in the cell liquid, collects the impurities in the impurity removal box, and avoids the influence of excessive impurities on the accuracy of observing the growth condition of the cells in the cell liquid.
Description
Technical Field
The invention relates to the field of cell culture equipment, in particular to an incubator with a detection function.
Background
The Chinese patent with the application number of CN201510668681.3 discloses a cell incubator, wherein the conditions of cell culture are defaulted by a display module, and then the conditions are controlled by a microprocessor operation, so that a stepper motor pumps culture solution into a culture bag;
meanwhile, in the process of cell growth, the cells can generate impurities such as excrement, when the impurities are too much, the absorption of the cells to the culture solution is easy to influence, so that the cells are in malnutrition and other conditions, further, new cell samples need to be re-cultured, the cultivation cost of the cells is increased, and the follow-up experimental process is influenced.
Disclosure of Invention
The invention provides an incubator with a detection function, which aims to overcome the defects that the growth condition of cells can be observed only after an incubator is opened to take out a culture bag, but when the culture bag is taken out of the incubator to be in contact with the outside, the observation result is easily inaccurate due to the influence of the outside factors, the representativeness of the observation result is reduced, meanwhile, in the process of cell growth, the cells can produce impurities such as excreta, and the like, when the impurities are excessive, the absorption of the cells to the culture solution is easily influenced, and the conditions such as malnutrition and the like of the cells are caused.
The technical implementation scheme of the invention is as follows: an incubator with a detection function comprises an incubator, a mounting rack and a cell observation platform; the inner side of the incubator is provided with a mounting rack; a cell observation table is arranged on the incubator; the device also comprises a clamp, a sampling tube, a power assembly, a guide assembly and a reflux system; the mounting frame is provided with a plurality of clamps for clamping the culture bags; a plurality of fixtures are provided with culture bags; a power assembly is arranged on the inner side of the incubator; the clamp is connected with the power assembly, and the power assembly drives the clamp to move back and forth and move left and right; a plurality of sampling tubes for sucking cell liquid in the culture bag are arranged above the mounting frame; a guide component for guiding the flowing direction of the cell fluid is arranged on the inner side of the incubator; the inner side of the incubator is provided with a reflux system for filling culture solution.
Optionally, the guiding component comprises a first four-way pipe fitting, a first three-way pipe fitting and a bending pipe fitting; the mounting frame is fixedly connected with a first four-way pipe fitting which is communicated with the sampling pipe; a first three-way pipe fitting is arranged on the inner side of the incubator; the first tee pipe fitting is communicated with the power assembly through a first water pipe; the upper side of the incubator is fixedly connected with a bending pipe fitting; the bending pipe fitting is communicated with the first tee pipe fitting through a second water pipe; the junction between buckling pipe fitting and the first tee bend pipe fitting is provided with first ball valve.
Optionally, a first branch pipe is also included; and each sampling tube is communicated with a plurality of first branch pipelines.
Optionally, the device also comprises a impurity removal box and a filter membrane; the mounting frame is provided with a impurity removing box; the first four-way pipe fitting is communicated with the impurity removal box through a third water pipe; the impurity removing box is communicated with the power assembly through a second water pipe; the junction between edulcoration case and the fourth water pipe is provided with the filter membrane that is arranged in the clearance cell sap impurity.
Optionally, the reflux system comprises a second four-way pipe fitting, a reflux pipe and a second branch pipe; the mounting frame is provided with a second four-way pipe fitting; the second four-way pipe fitting is communicated with the first three-way pipe fitting through a fifth water pipe; a plurality of return pipes are communicated with the second four-way pipe fitting; and each return pipe is communicated with a plurality of second branch pipelines.
Optionally, a supply pipe is also included; the incubator is communicated with a supply pipe for supplementing culture solution; the supply pipe is communicated with the fifth water pipe.
Optionally, a protector is also included; a plurality of protectors for preventing the culture bag from shaking are arranged on the lower side of the mounting frame; each protector is positioned outside the culture bag.
Optionally, the device also comprises a sponge block; each protector is provided with a sponge block for cleaning water stains on the surface of the culture bag; each sponge block is provided with a contact part; the contact part is attached to the outer surface of the culture bag.
Optionally, the device also comprises an elastic piece; a plurality of elastic pieces are fixedly connected between the uppermost protector and the sponge block.
Optionally, the cleaning device further comprises a cleaning assembly, wherein the cleaning assembly comprises a driving unit, a water tank, a water return pipe, a cleaning pipe, a one-way valve and a second three-way pipe fitting; a water tank is arranged at the inner side of the incubator; the water tank is communicated with a water return pipe; the water return pipe is communicated with a second tee pipe fitting; a second ball valve is arranged at the joint of the water return pipe and the second three-way pipe fitting; the second three-way pipe fitting is connected with a driving unit, and the driving unit is used for controlling the water circulation in the water tank; the impurity removing box is communicated with a cleaning pipe; the cleaning pipe is connected with the driving unit; a one-way valve is fixedly connected at the joint between the impurity removing box and the third water pipe; the one-way valve is arranged to open towards the interior of the impurity removal box.
The invention has the beneficial effects that: the invention realizes the filtration of the cell liquid by the filter membrane, realizes the interception of impurities such as excreta of cells in the cell liquid, collects the impurities in the impurity removal box, and avoids the influence of excessive impurities on the accuracy of observing the growth condition of the cells in the cell liquid;
expanding the culture bag through the first branch pipeline to avoid the culture bag being extruded into a flat state, so that cells are extruded and crushed, and meanwhile, increasing the contact area with cell fluid through the first branch pipeline, extracting the residual cell fluid, and avoiding the impurities such as excrement and the like in the residual cell fluid to influence the normal growth of the cells;
the culture bag is protected by the protector, so that the toppling of the culture bag is prevented, cell sap is prevented from being spilled out, and the normal growth of cells in the culture process is further ensured;
through the sponge piece sliding from top to bottom at the culture bag surface, realize the absorption to culture bag surface drop, avoid the culture bag surface to adsorb too much drop, influence the getting of staff and put.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional structure of an incubator with detection function according to the present invention;
FIG. 2 is a schematic perspective view of a first portion of the present invention;
FIG. 3 is a schematic perspective view of a second portion of the present invention;
FIG. 4 is a schematic perspective view showing the combination of a sampling tube and a first branch pipe according to the present invention;
FIG. 5 is a schematic view showing a combined three-dimensional structure of a sampling tube, a first four-way pipe fitting and a impurity removal box according to the present invention;
FIG. 6 is a schematic perspective view of a reflow system according to the present invention;
FIG. 7 is a schematic view of a portion of a reflow system of the present invention;
FIG. 8 is a schematic perspective view showing the combination of the protector, sponge block and elastic member of the present invention;
fig. 9 is a schematic perspective view of a cleaning assembly according to the present invention.
Reference numerals illustrate: 1-incubator, 2-mounting rack, 3-cell observation table, 4-clamp, 5-sampling tube, 101-slide rail, 102-electric slider, 103-electric push rod, 104-first driving piece, 201-first branch pipe, 301-first four-way pipe, 302-impurity removal box, 303-filter membrane, 304-first three-way pipe, 305-bending pipe, 401-second four-way pipe, 402-supply pipe, 403-return pipe, 404-second branch pipe, 405-protector, 406-sponge block, 40601-contact part, 407-elastic piece, 501-water tank, 502-return pipe, 503-cleaning pipe, 504-one-way valve, 505-second three-way pipe, 506-second driving piece.
Detailed Description
The invention is further described below with reference to the drawings and the detailed description.
Example 1
As shown in fig. 2 to 5, an incubator with a detection function comprises an incubator 1, a mounting rack 2 and a cell observation table 3; the inner side of the incubator 1 is provided with a mounting frame 2; a cell observation table 3 is arranged on the incubator 1;
the device also comprises a clamp 4, a sampling tube 5, a power component, a guide component and a reflux system; two clamps 4 which are bilaterally symmetrical are arranged on the mounting frame 2; two clamps 4 are provided with culture bags; a power assembly is arranged on the inner side of the incubator 1; the clamp 4 is connected with the power assembly, and the power assembly drives the clamp 4 to move back and forth and move left and right; a plurality of sampling pipes 5 are arranged above the mounting frame 2; a guide component is arranged on the inner side of the incubator 1; a reflux system is arranged on the inner side of the incubator 1.
The guiding assembly comprises a first four-way pipe fitting 301, a first three-way pipe fitting 304 and a bending pipe fitting 305; the mounting frame 2 is fixedly connected with a first four-way pipe fitting 301, and the first four-way pipe fitting 301 is communicated with the sampling pipe 5; a first three-way pipe fitting 304 is arranged on the inner side of the incubator 1; the first three-way pipe fitting 304 is communicated with the power assembly through a first water pipe; the upper side of the incubator 1 is fixedly connected with a bending pipe fitting 305; the bent pipe fitting 305 is communicated with the first three-way pipe fitting 304 through a second water pipe; a first ball valve is provided at the junction between the bent pipe fitting 305 and the first tee pipe fitting 304.
A first branch pipe 201 is also included; each sampling tube 5 is communicated with a plurality of first branch pipes 201.
The device also comprises a impurity removal box 302 and a filter membrane 303; the impurity removing box 302 is arranged on the mounting frame 2; the first four-way pipe fitting 301 is communicated with the impurity removal box 302 through a third water pipe; the impurity removal box 302 is communicated with the power component through a second water pipe; a filter membrane 303 is arranged at the joint between the impurity removal box 302 and the fourth water pipe.
The power assembly comprises a sliding rail 101, an electric sliding block 102, an electric push rod 103 and a first driving piece 104; two sliding rails 101 are arranged on the mounting frame 2; each sliding rail 101 is connected with an electric sliding block 102 in a sliding way; each electric sliding block 102 is fixedly connected with an electric push rod 103; each electric push rod 103 is fixedly connected with the adjacent fixture 4; a first driving piece 104 is arranged on the inner side of the incubator 1, and the first driving piece 104 is a water pump; the first driving piece 104 is communicated with the first three-way pipe fitting 304 through a first water pipe; the first driving piece 104 is communicated with the impurity removal box 302 through a fourth water pipe; the electric push rod 103 is driven by the electric slide block 102, the clamp 4 is driven to move back and forth along the slide rail 101, the clamp 4 is driven to squeeze the culture bag by the expansion of the electric push rod 103, the culture bag is clamped, the cell liquid is pumped into the cell observation table 3 by the first driving piece 104, and the cell liquid is sampled.
Firstly, a culture bag is placed on a clamp 4 manually, the clamp 4 is driven by the electric push rod 103 to squeeze the culture bag, the clamping of the culture bag is realized, when cells are cultured in the incubator 1 for the fifth day, a small amount of cell liquid is pumped into a first four-way pipe fitting 301 by a first driving member 104 through a sampling pipe 5, then into a impurity removing box 302 through a third water pipe, then into the first driving member 104 through a fourth water pipe, in the process, the cell liquid is filtered through a filter membrane 303 positioned at the joint between the impurity removing box 302 and the fourth water pipe, the interception of impurities such as excreta of cells in the cell liquid is realized, the impurities are collected in the impurity removing box 302, then the cell liquid is introduced into a first three-way pipe fitting 304 through the first water pipe, then a first ball valve positioned at the joint between the bending pipe fitting 305 and the first three-way pipe fitting 304 is opened, the cell liquid is led into the bending pipe fitting 305 along the second water pipe and then is led into the cell observation table 3, the growth condition of cells is detected by the cell observation table 3, the periodic detection of the cell growth condition is realized, when excessive impurities in the cell liquid are detected, all the cell liquid in the culture bag is pumped out by the sampling pipe 5, meanwhile, the air in the culture bag is greatly reduced in the pumping process of the cell liquid, the inside of the culture bag gradually forms a vacuum state, the culture bag is further influenced by the external pressure, the culture bag is extruded inwards, the residual cell liquid in the culture bag is difficult to pump, the culture bag is expanded by the first branch pipe 201, the culture bag is prevented from being extruded into a flat state, the cells are further crushed by extrusion, the contact area between the cell liquid and the cell liquid is increased by the first branch pipe 201, the residual cell liquid is pumped, avoiding residual cell fluid and excrement in the culture bag from affecting the normal growth of subsequent cells.
Example 2
On the basis of the embodiment 1, as shown in fig. 6 to 8, the reflux system comprises a second four-way pipe 401, a reflux pipe 403 and a second branch pipe 404; the second four-way pipe fitting 401 is installed on the installation frame 2; the second four-way pipe fitting 401 is communicated with the first three-way pipe fitting 304 through a fifth water pipe; a plurality of return pipes 403 are communicated with the second four-way pipe fitting 401; each return pipe 403 is connected to a plurality of second branch pipes 404.
Also included is supply line 402; the incubator 1 is communicated with a supply pipe 402; the supply line 402 communicates with a fifth water line.
Also included is guard 405; at least three protectors 405 are mounted on the underside of the mounting frame 2; each guard 405 is located outside the culture bag.
Also included is a sponge block 406; each protector 405 is provided with a sponge block 406; each sponge block 406 is provided with a contact portion 40601; the contact portion 40601 is attached to the outer surface of the culture bag.
Also included is an elastic member 407; a plurality of elastic pieces 407 are fixedly connected between the uppermost protector 405 and the sponge block 406, and the elastic pieces 407 are springs.
When the cell malnutrition is detected, the first driving part 104 is controlled to pump out all the cell liquid in the culture bag through the sampling pipe 5, then the first ball valve positioned at the joint between the bending pipe 305 and the first three-way pipe 304 is closed, the pumped-out culture liquid is led into the second four-way pipe 401 along the fifth water pipe, meanwhile, the culture liquid is led into the fifth water pipe through the supplying pipe 402, the cell liquid is supplemented, the pumped-out cell liquid and the supplemented culture liquid are synchronously led into the culture bag through the return pipe 403, the malnutrition of the cell caused by the shortage of the culture liquid is avoided, the subsequent growth of the cell is influenced, the normal growth of the cell in the culture process is ensured, when a worker touches the culture box 1 by mistake in the working process, the culture bag is protected through the protector 405, the cell liquid is prevented from being spilled out, further ensures the normal growth of cells in the culture process, because the cells need to be cultured in a high-humidity environment, the surface of the culture bag is easy to condense water drops, the culture bag gradually forms a vacuum state in the process that culture solution is pumped out of the culture bag, the culture bag is influenced by external pressure, the culture bag is extruded inwards, a gap is further generated between the contact part 40601 and the culture bag, the friction force between the sponge block 406 and the outer surface of the culture bag is reduced, the sponge block 406 slides downwards, the contact part 40601 is further attached to the outer surface of the culture bag again, the adsorption of the water drops on the surface of the culture bag is realized, the culture bag gradually expands from bottom to top in the process that the culture solution is supplemented into the culture bag, the sponge block 406 slides upwards through the culture bag extrusion contact part 40601, the adsorption of the water drops on the surface of the culture bag is realized, the excessive water drops condensation on the surface of the culture bag is avoided, the staff is influenced to get to cultivate the bag and put, meanwhile, through the elastic component 407 that is located between protector 405 and the sponge piece 406 of topside, extrude back flow 403 at the in-process of cultivateing the bag inflation, make second branch pipeline 404 buckle to cultivate the bag inner wall, and then make second branch pipeline 404 and cultivate bag inner wall contact, at the in-process of injecting into the culture solution, reduce the production of bubble, drive electric putter 103 and then drive anchor clamps 4 along slide rail 101 back-and-forth movement through electric slider 102, make back flow 403 produce the beat, and then make second branch pipeline 404 synchronous beat in the culture solution, realize the elimination to the bubble, avoid the bubble too much to influence the normal growth of cell.
Example 3
On the basis of the embodiments 1 and 2, as shown in fig. 1, 5 and 9, the cleaning assembly further comprises a cleaning assembly, wherein the cleaning assembly comprises a driving unit, a water tank 501, a water return pipe 502, a cleaning pipe 503, a one-way valve 504 and a second three-way pipe 505; a water tank 501 is installed inside the incubator 1; a water return pipe 502 is communicated with the water tank 501; the return pipe 502 is communicated with a second three-way pipe fitting 505; a second ball valve is arranged at the joint of the water return pipe 502 and the second three-way pipe fitting 505; a driving unit is connected to the second three-way pipe fitting 505, and water circulation in the water tank 501 is controlled by the driving unit; the impurity removal box 302 is communicated with a cleaning pipe 503; the cleaning tube 503 is connected with the driving unit; a one-way valve 504 is fixedly connected at the joint between the impurity removing box 302 and the third water pipe; the one-way valve 504 is configured to open toward the interior of the decontamination box 302.
The driving unit comprises a second driving piece 506; the second three-way pipe fitting 505 is communicated with a second driving piece 506, and the second driving piece 506 is a water pump; the second driving piece 506 is communicated with the second three-way pipe 505; the second driving member 506 communicates with the purge tube 503; the circulation of water in the water tank 501 is controlled by the second driving member 506.
In the process of expanding the culture bag, the culture bag is outwards extruded to be in contact with the protector 405, when the culture bag is continuously expanded, water drops adsorbed in the sponge block 406 are extruded through the extrusion of the culture bag and the protector 405, and then circulate into the water tank 501 along the outer wall of the culture bag, after the culture liquid flows back to the culture bag, the second ball valve is opened, water in the water tank 501 is introduced into the cleaning pipe 503 along the water return pipe 502 through the second driving piece 506, and then the water is introduced into the impurity removing box 302, so that impurities adsorbed in the impurity removing box 302 and on the filter membrane 303 are cleaned, and meanwhile, the third water pipe is blocked through the one-way valve 504, water flow is prevented from being introduced into the culture bag along the third water pipe, so that the water flow with the impurities is prevented from polluting cell liquid in the culture bag, normal operation of cell culture is further ensured, after the cleaning is finished, the second ball valve is closed, the water flow with the impurities in the impurity removing box 302 is controlled to be introduced into the second three-way pipe 505 along the cleaning pipe 503, and then the water with the impurities is discharged through the second three-way pipe 505.
While the present invention has been described in detail with reference to the above embodiments, it will be apparent to those skilled in the art from this disclosure that various changes or modifications can be made therein without departing from the spirit and scope of the invention as defined in the following claims. Accordingly, the detailed description of the disclosed embodiments is to be taken only by way of illustration and not by way of limitation, and the scope of protection is defined by the content of the claims.
Claims (10)
1. An incubator with a detection function comprises an incubator (1), a mounting rack (2) and a cell observation table (3); the inner side of the incubator (1) is provided with a mounting rack (2); a cell observation table (3) is arranged on the incubator (1); the method is characterized in that: the device also comprises a clamp (4), a sampling tube (5), a power assembly, a guide assembly and a reflux system; a plurality of clamps (4) for clamping the culture bags are arranged on the mounting frame (2); a plurality of fixtures (4) are provided with culture bags; a power assembly is arranged on the inner side of the incubator (1); the clamp (4) is connected with the power assembly, and the power assembly drives the clamp (4) to move forwards and backwards and left and right; a plurality of sampling tubes (5) for sucking the cell liquid in the culture bag are arranged above the mounting frame (2); a guide component for guiding the flowing direction of the cell fluid is arranged on the inner side of the incubator (1); the inner side of the incubator (1) is provided with a reflux system for filling culture solution.
2. An incubator with a detection function according to claim 1, characterized in that: the guiding assembly comprises a first four-way pipe fitting (301), a first three-way pipe fitting (304) and a bending pipe fitting (305); the mounting frame (2) is fixedly connected with a first four-way pipe fitting (301), and the first four-way pipe fitting (301) is communicated with the sampling pipe (5); a first three-way pipe fitting (304) is arranged on the inner side of the incubator (1); the first tee pipe fitting (304) is communicated with the power assembly through a first water pipe; the upper side of the incubator (1) is fixedly connected with a bending pipe fitting (305); the bending pipe fitting (305) is communicated with the first three-way pipe fitting (304) through a second water pipe; the joint between the bending pipe fitting (305) and the first tee pipe fitting (304) is provided with a first ball valve.
3. An incubator with a detection function as claimed in claim 2, characterized in that: also comprises a first branch pipeline (201); each sampling tube (5) is communicated with a plurality of first branch pipes (201).
4. An incubator with a detection function as claimed in claim 2, characterized in that: the device also comprises a impurity removal box (302) and a filter membrane (303); the mounting frame (2) is provided with a impurity removing box (302); the first four-way pipe fitting (301) is communicated with the impurity removal box (302) through a third water pipe; the impurity removing box (302) is communicated with the power assembly through a second water pipe; a filter membrane (303) for cleaning impurities in the cell sap is arranged at the joint between the impurity removal box (302) and the fourth water pipe.
5. An incubator with a detection function according to claim 4, characterized in that: the reflux system comprises a second four-way pipe fitting (401), a reflux pipe (403) and a second branch pipeline (404); the mounting frame (2) is provided with a second four-way pipe fitting (401); the second four-way pipe fitting (401) is communicated with the first three-way pipe fitting (304) through a fifth water pipe; a plurality of return pipes (403) are communicated with the second four-way pipe fitting (401); each return pipe (403) is communicated with a plurality of second branch pipelines (404).
6. An incubator with a detection function as claimed in claim 2, characterized in that: also comprises a supply pipe (402); a supply pipe (402) for supplementing culture solution is communicated with the incubator (1); the supply pipe (402) is communicated with the fifth water pipe.
7. An incubator with detection function according to claim 5, characterized in that: also comprises a protector (405); a plurality of protectors (405) for preventing the culture bag from shaking are arranged on the lower side of the mounting frame (2); each guard (405) is located outside the culture bag.
8. An incubator with detection function according to claim 7, characterized in that: also comprises a sponge block (406); each protector (405) is provided with a sponge block (406) for cleaning water stains on the surface of the culture bag; each sponge block (406) is provided with a contact part (40601); the contact part (40601) is attached to the outer surface of the culture bag.
9. An incubator with a detection function as set forth in claim 8, wherein: also comprises an elastic piece (407); a plurality of elastic pieces (407) are fixedly connected between the uppermost protector (405) and the sponge block (406).
10. An incubator with detection function according to claim 6, characterized in that: the cleaning assembly comprises a driving unit, a water tank (501), a water return pipe (502), a cleaning pipe (503), a one-way valve (504) and a second three-way pipe fitting (505); a water tank (501) is arranged on the inner side of the incubator (1); a water return pipe (502) is communicated with the water tank (501); the water return pipe (502) is communicated with a second three-way pipe fitting (505); a second ball valve is arranged at the joint of the water return pipe (502) and the second three-way pipe fitting (505); the second three-way pipe fitting (505) is connected with a driving unit, and the driving unit controls the water circulation in the water tank (501); a cleaning pipe (503) is communicated with the impurity removal box (302); the cleaning pipe (503) is connected with the driving unit; a one-way valve (504) is fixedly connected at the joint between the impurity removal box (302) and the third water pipe; the one-way valve (504) is arranged to open towards the interior of the decontamination box (302).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410152967.5A CN117683635B (en) | 2024-02-04 | 2024-02-04 | Incubator with detect function |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410152967.5A CN117683635B (en) | 2024-02-04 | 2024-02-04 | Incubator with detect function |
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| CN117683635A true CN117683635A (en) | 2024-03-12 |
| CN117683635B CN117683635B (en) | 2024-04-16 |
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205473766U (en) * | 2016-03-14 | 2016-08-17 | 上海康岱生物医药技术股份有限公司 | Cell culture bag sample pipeline |
| CN209468454U (en) * | 2019-01-23 | 2019-10-08 | 江苏省人民医院 | A kind of test anti-fungal interference cell incubator |
| JP2020061979A (en) * | 2018-10-18 | 2020-04-23 | ルン−クァン リュＬｕｎ−Ｋｕａｎｇ Ｌｉｕ | Cell incubator |
| CN212476777U (en) * | 2020-06-04 | 2021-02-05 | 青岛环湾检测评价股份有限公司 | Cell culture blending device used in incubator |
| CN114164108A (en) * | 2021-12-17 | 2022-03-11 | 昆明医科大学 | Cell culture device capable of monitoring primary nasopharyngeal carcinoma cell culture liquid environment |
| CN217600745U (en) * | 2022-01-21 | 2022-10-18 | 上海赛岚生物科技有限公司 | Biological cell incubator for biological assay |
| CN217677537U (en) * | 2022-04-22 | 2022-10-28 | 中南大学 | Oxygen supply adjustable cell culture device for medical science experiments |
| CN219032214U (en) * | 2023-01-05 | 2023-05-16 | 中博瑞康(上海)生物技术有限公司 | Sampling device for disposable cell culture bag |
| CN219156892U (en) * | 2023-02-21 | 2023-06-09 | 翔鹏佑康(北京)科技有限公司 | Culture apparatus is used in preparation of CAR-NK cell |
| WO2023116833A1 (en) * | 2021-12-22 | 2023-06-29 | 基亚生物科技股份有限公司 | Integrated automated cell culture device |
| CN219907702U (en) * | 2023-05-31 | 2023-10-27 | 上海中成诺舟生物科技有限公司 | Immune cell culture bag convenient to sample |
-
2024
- 2024-02-04 CN CN202410152967.5A patent/CN117683635B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205473766U (en) * | 2016-03-14 | 2016-08-17 | 上海康岱生物医药技术股份有限公司 | Cell culture bag sample pipeline |
| JP2020061979A (en) * | 2018-10-18 | 2020-04-23 | ルン−クァン リュＬｕｎ−Ｋｕａｎｇ Ｌｉｕ | Cell incubator |
| CN209468454U (en) * | 2019-01-23 | 2019-10-08 | 江苏省人民医院 | A kind of test anti-fungal interference cell incubator |
| CN212476777U (en) * | 2020-06-04 | 2021-02-05 | 青岛环湾检测评价股份有限公司 | Cell culture blending device used in incubator |
| CN114164108A (en) * | 2021-12-17 | 2022-03-11 | 昆明医科大学 | Cell culture device capable of monitoring primary nasopharyngeal carcinoma cell culture liquid environment |
| WO2023116833A1 (en) * | 2021-12-22 | 2023-06-29 | 基亚生物科技股份有限公司 | Integrated automated cell culture device |
| CN217600745U (en) * | 2022-01-21 | 2022-10-18 | 上海赛岚生物科技有限公司 | Biological cell incubator for biological assay |
| CN217677537U (en) * | 2022-04-22 | 2022-10-28 | 中南大学 | Oxygen supply adjustable cell culture device for medical science experiments |
| CN219032214U (en) * | 2023-01-05 | 2023-05-16 | 中博瑞康(上海)生物技术有限公司 | Sampling device for disposable cell culture bag |
| CN219156892U (en) * | 2023-02-21 | 2023-06-09 | 翔鹏佑康(北京)科技有限公司 | Culture apparatus is used in preparation of CAR-NK cell |
| CN219907702U (en) * | 2023-05-31 | 2023-10-27 | 上海中成诺舟生物科技有限公司 | Immune cell culture bag convenient to sample |
Non-Patent Citations (1)
| Title |
|---|
| 都田赵 等: "两种培养袋对细胞因子诱导的杀伤细胞分化增殖作用的比较", 中国生物制品学杂志, no. 01, 31 December 2018 (2018-12-31), pages 29 - 32 * |
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