CN1176360C - Method of preparing multi tissue paraffin embedded wax lump of tissue chip and its device - Google Patents
Method of preparing multi tissue paraffin embedded wax lump of tissue chip and its device Download PDFInfo
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- CN1176360C CN1176360C CNB021394741A CN02139474A CN1176360C CN 1176360 C CN1176360 C CN 1176360C CN B021394741 A CNB021394741 A CN B021394741A CN 02139474 A CN02139474 A CN 02139474A CN 1176360 C CN1176360 C CN 1176360C
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- water bath
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Abstract
The present invention discloses a method of preparing multi-tissue paraffin embedding wax blocks suitable for tissue chip preparation and a device for realizing the method. In the present invention, the temperature for melting paraffin is controlled to be slightly higher than the melting point within the range between 5 DEG C and 10 DEG C by a constant temperature water bath box with a temperature control switch; the embedding temperature is controlled within the range of the paraffin melting point through a constant temperature water bath tank below an embedding tank; certain negative pressure is formed at the bottom of the embedding tank through a negative pressure pump communicated with a negative pressure box below the embedding tank. The present invention has the purposes that air between a tissue sample and a thin layer wax block blank is sucked out at the same time of adding melted paraffin and the melted paraffin are quickly filled in the gaps between the tissue sample and the thin layer wax block to form tightly combined integration. In addition, a plurality of tissue samples in the thin layer wax block blank can be simultaneously and firmly adsorbed on a yarn net at the bottom of the embedding tank by negative pressure to achieve the purposes of positioning and embedding. The device and the method of the present invention can conveniently and quickly prepare high quality multi-tissue paraffin imbedding wax blocks.
Description
Technical field
The invention belongs to the making and the method thereof of the organization chip of medical domain, the device more particularly a kind of making can be used for preparing the method for organizing the paraffin embedding wax stone of organization chip and realize this method.Method and apparatus of the present invention can be widely used in the preparation process of organization chip.
Background technology
In the process of preparation organization chip, at first must make and organize the paraffin embedding wax stone more, and organize the quality of the relationship between quality of paraffin embedding wax stone to organization chip more, when the preparation organization chip, how to guarantee the accurate location embedding of many tissue samples and remove tissue samples and the wax stone blank between air, making tissue samples and thin layer wax stone form complete integral body, is the difficult problem of many organization embeddings.This difficulty increases along with the increase of the quantity of investing tissue's sample.
According to present existing technology, when the thin layer wax stone that directly will have many tissue samples is put into the paraffin of fusing,, be difficult for smooth sinking at the bottom of the embedding frame because the thin layer wax stone that solidifies is lighter, perhaps can not make it parallel with the embedding frame bottom, make tissue samples be in same plane, and, if the temperature of the paraffin of fusing is too high, thin layer paraffin blank will be melted, arrange good tissue samples in it since its density less, just floating, move, can not reach the purpose of location embedding; If temperature is low excessively, make the paraffin of fusing outside thin layer paraffin, form one deck paraffin shell.In addition, if the air between tissue samples and the thin layer paraffin can not effectively be got rid of, tissue samples and thin layer wax stone blank can not form complete integral body, cause section imperfect.
According to present existing technology, when directly joining the paraffin that melts on the thin layer wax stone that has many tissue samples, same reason, if the temperature of the paraffin of fusing is too high, thin layer paraffin blank will be melted, arrange good tissue samples in it because its density is less, just floating, move, can not reach the purpose of location embedding,, make the paraffin of fusing outside thin layer paraffin, form one deck paraffin shell if temperature is low excessively, in addition, air between tissue samples and the thin layer paraffin can not be excluded, and tissue samples and thin layer wax stone blank can not form complete integral body, causes section imperfect.
As seen, adopt present existing technology, all can not finish the quick preparation of organizing the paraffin embedding wax stone smoothly more, and guarantee that the interior tissue samples of wax stone of preparation is in same level, and accurate positioning, combine closely with wax stone.
Summary of the invention
The temperature and the embedding temperature of the present invention and the melted paraffin of control when organizing the paraffin embedding wax stone to prepare more and control the location embedding of many tissue samples and remove tissue samples and thin layer wax stone blank between the method and apparatus of air relevant.Defective or deficiency at above-mentioned prior art existence, an object of the present invention is, provide a kind of making to can be used for preparing the method for organizing the paraffin embedding wax stone of organization chip more, this method can be eliminated above-mentioned defective, particularly eliminate preparation include greater number tissue samples organize the paraffin embedding wax stone more the time prior art shortcoming that exists.Another object of the present invention is, provides and realizes the used device of said method.
To achieve these goals, the technical scheme that the present invention takes is that a kind of method of organizing the paraffin embedding wax stone for preparing organization chip comprises the following steps: more
1) medical paraffin wax is held in place in the wax cylinder that has temp control switch in the constant water bath box, makes medical paraffin wax keep molten state, and the paraffin that melts can flow out to through the conduit of wax cylinder bottom portion in the embedding frame of below;
2) open the switch of constant temperature water bath, make the temperature of embedding groove rise to 55 ℃～60 ℃;
3) that will prepare and landfill have the thin layer paraffin wax stone blank that is used to prepare organization chip of a plurality of tissue samples to be placed in the embedding frame;
4) the embedding frame that will place the wax stone blank is placed in the embedding groove;
5) the embedding groove together is positioned on the negative pressure box that is positioned at constant temperature water bath together with the embedding frame that has placed the wax stone blank in it, and makes it involutory good;
6) open branch's exhaust tube switch of the negative pressure pump that is communicated with negative pressure box, open the negative pressure pump power switch again, and its pressure is adjusted to 1/2 atmospheric pressure, open the mozzle switch of the wax cylinder bottom portion on embedding frame top simultaneously, the paraffin that adds fusing, by the negative pressure swabbing action, remove the air between fritter tissue samples and the thin layer paraffin blank, behind the paraffin filling tissue samples and the space between the paraffin blank of fusing, close the mozzle switch of the wax cylinder bottom portion on branch's exhaust tube switch of negative pressure pump power switch, negative pressure pump and embedding frame top successively;
7) close constant water bath box and Water Tank with Temp.-controlled switch, wait embedding groove temperature to be reduced to room temperature after, the wax stone in the embedding frame is taken out, what being used to of can obtaining preparing prepared organization chip organizes the paraffin embedding wax stone more.
Realize the device of organizing paraffin embedding wax stone method of above-mentioned preparation organization chip, this device comprises more:
A wax cylinder, it is positioned at one and has temp control switch and by the heat supply of constant water bath box heating wire with by the draining of constant water bath box aqueduct and have the constant water bath box of constant water bath box aqueduct switch and lid, and there is a wax cylinder mozzle that has a mozzle switch that passes from constant water bath box in wax cylinder bottom portion;
Temp control switch that has a constant temperature water bath and by the heat supply of constant temperature water bath heating wire with by the constant temperature water bath of constant temperature water bath aqueduct draining, constant temperature water bath aqueduct switch is arranged on the constant temperature water bath aqueduct, and negative pressure box is positioned at constant temperature water bath;
In constant temperature water bath, also be provided with:
Embedding frame and embedding groove, the base plate of each embedding groove is made by the sheet metal that the middle part has gauze, places an embedding frame in each embedding groove;
One or more negative pressure boxs, negative pressure box is corresponding one by one with the embedding groove, and the base plate of embedding groove is as the top cover of negative pressure box;
Outside constant temperature water bath, also be provided with:
A negative pressure pump that has the negative pressure pump pressure regulating switch, the base plate that is provided with branch's exhaust tubes of branch's exhaust tube switch and passes the embedding groove respectively by exhaust tube a series of and embedding groove negative pressure box one to one are communicated with;
Constant water bath box on constant temperature water bath, and is fixed in embedding groove top by support with the wax cylinder by stent support;
One power supply is connected with constant water bath box, constant temperature water bath and negative pressure pump respectively.
The present invention controls the temperature of melted paraffin just in time in 5 ℃～10 ℃ scopes of a little higher than its fusing point (56 ℃～58 ℃) by the constant water bath box that has temp control switch, control the scope of embedding temperature by the constant temperature water bath that is positioned under the embedding groove at paraffin melting point (56 ℃～58 ℃), by the negative pressure pump that is communicated with the negative pressure box of embedding groove below, form certain negative pressure at the embedding trench bottom, its objective is in the paraffin that adds fusing, the air between tissue samples and the thin layer wax stone blank is removed in suction, and fill rapidly between the two space with the paraffin of fusing, the integral body that its formation is combined closely, in addition, also can by negative pressure a plurality of tissue samples in the thin layer wax stone blank be adsorbed on the screen cloth of embedding trench bottom firmly simultaneously, reach the purpose of location embedding.
Description of drawings
Fig. 1 is the structural representation of organizing paraffin embedding wax stone device of organization chip of the present invention more; Each symbol among the figure is respectively: 1-wax cylinder, 2-temp control switch, 3-constant water bath box, 4-constant water bath box aqueduct, 5-constant water bath box aqueduct switch, 6-lid; 7-wax cylinder mozzle, 8-mozzle switch, 9-embedding frame, 10-embedding groove, 11-embedding trough floor, 12-gauze, 13-embedding groove dividing plate, 14-constant temperature water bath, the temp control switch of 15-constant temperature water bath, 16-constant temperature water bath aqueduct, 17-constant temperature water bath aqueduct switch, 18-negative pressure pump pressure regulating switch, the 19-negative pressure pump, 20-exhaust tube, 21-branch exhaust tube switch, 22-branch exhaust tube, 23-support, 24-power supply.
Fig. 2 is Fig. 1 coronal-plane sectional view; Each symbol among the figure is respectively: 1-wax cylinder, 3-constant water bath box, 4-constant water bath box aqueduct, 5-constant water bath box aqueduct switch, 6-lid; 7-wax cylinder mozzle, 8-wax cylinder mozzle switch, 9-embedding frame, 10-embedding groove, 11-embedding trough floor, 12-gauze, 13-embedding groove dividing plate, 14-constant temperature water bath, 16-constant temperature water bath aqueduct, 17-constant temperature water bath aqueduct switch, the 19-negative pressure pump, 25-constant water bath box heating wire, 26-negative pressure box, 27-constant temperature water bath heating wire, 28-embedding frame dividing plate.
Fig. 3 is the structural representation of constant temperature water bath; Each symbol among the figure is respectively: 14-constant temperature water bath, the temp control switch of 15-constant temperature water bath, 23-support.
Fig. 4 is the structural representation of embedding groove; Each symbol among the figure is respectively: 10-embedding groove, 11-embedding trough floor, 12-gauze, 13-embedding groove dividing plate, 20-exhaust tube, 21-branch exhaust tube switch, 22-branch exhaust tube.
Fig. 5 is a negative pressure box sagittal plane synoptic diagram; Each symbol is respectively among the figure: 10-embedding groove, 11-embedding trough floor, 12-gauze, 20-exhaust tube, 21-branch exhaust tube switch, 22-branch exhaust tube, 26-negative pressure box.
Fig. 6 is an embedding mount structure synoptic diagram; Each symbol among the figure is respectively: 9-embedding frame, 28-embedding frame dividing plate.
Embodiment
The present invention is described in further detail according to specific embodiment that technical scheme of the present invention is finished below in conjunction with accompanying drawing and inventor.
Referring to Fig. 1～6, Fig. 1～6 also are specific embodiments of the present invention, but the invention is not restricted to this embodiment.
By technical scheme of the present invention, the method for organizing the paraffin embedding wax stone of preparation organization chip comprises the following steps: more
1) medical paraffin wax is held in place in the wax cylinder that has temp control switch in the constant water bath box, makes medical paraffin wax keep molten state, and the paraffin that melts can flow out to through the mozzle of wax cylinder bottom portion in the embedding frame of below;
2) open the switch of constant temperature water bath, make the temperature of embedding groove rise to 55 ℃～60 ℃;
3) that will prepare and landfill have the thin layer paraffin wax stone blank that is used to prepare organization chip of a plurality of tissue samples to be placed in the embedding frame;
4) the embedding frame that will place the wax stone blank is placed in the embedding groove;
5) the embedding groove together is positioned on the negative pressure box that is positioned at constant temperature water bath together with the embedding frame that has placed the wax stone blank in it, and makes it involutory good;
6) open branch's exhaust tube switch of the negative pressure pump that is communicated with negative pressure box, open the negative pressure pump power switch again, and its pressure is adjusted to 1/2 atmospheric pressure, open the mozzle switch of the wax cylinder bottom portion on embedding frame top simultaneously, the paraffin that adds fusing, by the negative pressure swabbing action, remove the air between fritter tissue samples and the thin layer paraffin blank, behind the paraffin filling tissue samples and the space between the paraffin blank of fusing, close the mozzle switch of the wax cylinder bottom portion on branch's exhaust tube switch of negative pressure pump power switch, negative pressure pump and embedding frame top successively;
7) close constant water bath box and Water Tank with Temp.-controlled switch, wait embedding groove temperature to be reduced to room temperature after, the wax stone in the embedding frame is taken out, can obtain preparing be used to prepare organization chip organize the paraffin embedding wax stone more.
Realize the device of organizing paraffin embedding wax stone method of above-mentioned preparation organization chip, this device comprises more:
A wax cylinder 1, it is positioned at one and has temp control switch 2 and by 25 heat supplies of constant water bath box heating wire with by 4 drainings of constant water bath box aqueduct and have the constant water bath box 3 of constant water bath box aqueduct switch 5 and lid 6, and a wax cylinder mozzle 7 that has a mozzle switch 8 that passes from constant water bath box 3 is arranged at wax cylinder 1 bottom;
In constant temperature water bath 14, also be provided with:
Embedding frame 9 and embedding groove 10, the base plate 11 of each embedding groove 10 is made by the sheet metal that the middle part has gauze 12, places an embedding frame 9 in each embedding groove;
One or more negative pressure boxs 26, negative pressure box 26 is corresponding one by one with embedding groove 10, and the base plate 11 of embedding groove 10 is as the top cover of negative pressure box 26;
Outside constant temperature water bath 14, also be provided with:
A negative pressure pump 19 that has negative pressure pump pressure regulating switch 18, the base plate 11 that is provided with branch's exhaust tubes 22 of branch's exhaust tube switch 21 and passes embedding groove 10 respectively by exhaust tube 20 a series of and embedding groove 10 negative pressure box 26 one to one are communicated with;
Constant water bath box 3 is supported on the constant temperature water bath 14 by support 23, and by support 23 wax cylinder 1 is fixed in embedding groove 10 tops;
One power supply 24 is connected with constant water bath box 3, constant temperature water bath 14 and negative pressure pump 19 respectively.
Described embedding frame is constituted, surrounds cubic frame shape by four sidewalls,, can prepare the suitable device of organizing the paraffin embedding wax stone more according to accompanying drawing of the present invention.Device recited above can amplify and dwindle when making as required.
According to the mechanism of the invention described above, the method and apparatus of the application of the invention just can very conveniently be prepared the high-quality paraffin embedding wax stone of organizing fast.
Claims (2)
1. a method of organizing the paraffin embedding wax stone for preparing organization chip is characterized in that this method comprises the following steps: more
1) medical paraffin wax is held in place in the paraffin cylinder that has temp control switch in the constant water bath box, makes medical paraffin wax keep molten state, and the paraffin that melts can flow out to through the conduit of wax cylinder bottom portion in the embedding frame of below;
2) open the switch of constant temperature water bath, make the temperature of embedding groove rise to 55～60 ℃;
3) that will prepare and landfill have the thin layer paraffin wax stone blank that is used to prepare organization chip of a plurality of tissue samples to be placed in the embedding frame;
4) the embedding frame that will place the wax stone blank is placed in the embedding groove;
5) the embedding groove together is positioned on the negative pressure box that is positioned at constant temperature water bath together with the embedding frame that has placed the wax stone blank in it, and makes it involutory good;
6) open branch's exhaust tube switch of the negative pressure pump that is communicated with negative pressure box, open the negative pressure pump power switch again, and its pressure is adjusted to 1/2 atmospheric pressure, open the mozzle switch of the wax cylinder bottom portion on embedding frame top simultaneously, the paraffin that adds fusing, by the negative pressure swabbing action, remove the air between fritter tissue samples and the thin layer paraffin blank, behind the paraffin filling tissue samples and the space between the paraffin blank of fusing, close the mozzle switch of the wax cylinder bottom portion on branch's exhaust tube switch of negative pressure pump power switch, negative pressure pump and embedding frame top successively;
7) close constant water bath box and Water Tank with Temp.-controlled switch, wait embedding groove temperature to be reduced to room temperature after, the wax stone in the embedding frame is taken out, what being used to of can obtaining preparing prepared organization chip organizes the paraffin embedding wax stone more.
2. the device of the method for organizing the paraffin embedding wax stone of a described preparation organization chip that is used to realize claim 1 is characterized in that this device comprises more:
A wax cylinder (1), it is positioned at one and has temp control switch (2) and by heating wire (25) heat supply of constant water bath box with by (4) draining of constant water bath box aqueduct and have constant water bath box aqueduct switch (5) and the constant water bath box (3) of lid (6), and a wax cylinder mozzle (7) that has a mozzle switch (8) that passes from constant water bath box (3) is arranged at wax cylinder (1) bottom;
A constant temperature water bath (14), it has the temp control switch (15) of constant temperature water bath and by (27) heat supply of constant temperature water bath heating wire with by constant temperature water bath aqueduct (16) draining, constant temperature water bath aqueduct switch (17) is arranged on the constant temperature water bath aqueduct (16), and negative pressure box (26) is positioned at constant temperature water bath (14);
In constant temperature water bath (14), also be provided with:
Embedding frame (9) and embedding groove (10), the base plate (11) of each embedding groove (10) is made by the sheet metal that the middle part has gauze (12), places an embedding frame (9) in each embedding groove;
One or more negative pressure boxs (26), negative pressure box (26) is corresponding one by one with embedding groove (10), and the base plate (11) of embedding groove (10) is as the top cover of negative pressure box (26);
Outside constant temperature water bath (14), also be provided with:
A negative pressure pump (19) that has negative pressure pump pressure regulating switch (18), the base plate (11) that is provided with branch's exhaust tubes (22) of branch's exhaust tube switch (21) and passes embedding groove (10) respectively by exhaust tube (20) a series of and embedding groove (10) negative pressure box (26) one to one are communicated with;
Constant water bath box (3) is supported on the constant temperature water bath (14) by support (23), and by support (23) wax cylinder (1) is fixed in embedding groove (10) top;
One power supply (24) is connected with constant water bath box (3), constant temperature water bath (14) and negative pressure pump (19) respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021394741A CN1176360C (en) | 2002-10-18 | 2002-10-18 | Method of preparing multi tissue paraffin embedded wax lump of tissue chip and its device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021394741A CN1176360C (en) | 2002-10-18 | 2002-10-18 | Method of preparing multi tissue paraffin embedded wax lump of tissue chip and its device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1410750A CN1410750A (en) | 2003-04-16 |
| CN1176360C true CN1176360C (en) | 2004-11-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021394741A Expired - Fee Related CN1176360C (en) | 2002-10-18 | 2002-10-18 | Method of preparing multi tissue paraffin embedded wax lump of tissue chip and its device |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100353155C (en) * | 2004-09-17 | 2007-12-05 | 中国科学院海洋研究所 | Tissue section cutting method for sea water left eye floundre and right eye founder fertilized egg |
| US20060121597A1 (en) * | 2004-11-24 | 2006-06-08 | Rongshan Li | Cytoblock preparation system and methods of use |
| JP4059520B1 (en) * | 2007-07-25 | 2008-03-12 | 株式会社常光 | Tissue piece processing equipment |
| CN101556224B (en) * | 2009-05-18 | 2011-02-16 | 复旦大学附属中山医院 | Manufacturing method of paraffin tissue chip |
| CN102435725A (en) * | 2011-08-31 | 2012-05-02 | 复旦大学附属中山医院 | Simple inoculation method for paraffin tissue chip |
| CN102719352B (en) * | 2012-06-06 | 2014-01-29 | 西安交通大学 | Cell chip slide for preparing microarray cell chips and preparation method |
| CN102967493B (en) * | 2012-10-27 | 2015-04-08 | 山西农业大学 | Rapid paraffin sectioning method for plant tissue |
| CN103900864A (en) * | 2012-12-25 | 2014-07-02 | 泰州医药城博奥邦科生物科技有限公司 | Exfoliated cell chip |
| CN104390837B (en) * | 2014-10-22 | 2017-01-18 | 中国人民解放军总医院第一附属医院 | Tissue embedding instrument capable of automatically adjusting temperature |
| CN105779973A (en) * | 2014-12-23 | 2016-07-20 | 中国电子科技集团公司第十八研究所 | Constant temperature water bath apparatus for chemical plating |
| CN106290696B (en) * | 2015-06-23 | 2023-04-11 | 中美华世通生物医药科技(武汉)股份有限公司 | Thin-layer chromatography system and chromatography method |
| CN107463193B (en) * | 2017-08-30 | 2022-09-09 | 中国医科大学附属第一医院 | Low temperature tissue embedding temperature control system |
-
2002
- 2002-10-18 CN CNB021394741A patent/CN1176360C/en not_active Expired - Fee Related
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| CN1410750A (en) | 2003-04-16 |
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