CN117619463A - New crown detection micro-fluidic device based on coagulation dysfunction - Google Patents
New crown detection micro-fluidic device based on coagulation dysfunction Download PDFInfo
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- CN117619463A CN117619463A CN202311568593.7A CN202311568593A CN117619463A CN 117619463 A CN117619463 A CN 117619463A CN 202311568593 A CN202311568593 A CN 202311568593A CN 117619463 A CN117619463 A CN 117619463A
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- 238000001514 detection method Methods 0.000 title claims abstract description 84
- 230000004064 dysfunction Effects 0.000 title claims abstract description 6
- 230000015271 coagulation Effects 0.000 title abstract description 3
- 238000005345 coagulation Methods 0.000 title abstract description 3
- 239000000523 sample Substances 0.000 claims abstract description 59
- 238000001914 filtration Methods 0.000 claims abstract description 42
- 239000000758 substrate Substances 0.000 claims abstract description 36
- 239000002699 waste material Substances 0.000 claims abstract description 31
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 229920000742 Cotton Polymers 0.000 claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000012530 fluid Substances 0.000 claims abstract description 9
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 9
- 230000023555 blood coagulation Effects 0.000 claims abstract description 3
- 206010053567 Coagulopathies Diseases 0.000 claims abstract 3
- -1 polydimethylsiloxane Polymers 0.000 claims description 10
- 238000002493 microarray Methods 0.000 claims description 9
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 9
- 229920000139 polyethylene terephthalate Polymers 0.000 claims description 9
- 239000005020 polyethylene terephthalate Substances 0.000 claims description 9
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 9
- 239000004793 Polystyrene Substances 0.000 claims description 7
- 229920000515 polycarbonate Polymers 0.000 claims description 7
- 239000004417 polycarbonate Substances 0.000 claims description 7
- 229920002223 polystyrene Polymers 0.000 claims description 7
- 108090000190 Thrombin Proteins 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 229960004072 thrombin Drugs 0.000 claims description 6
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 238000003745 diagnosis Methods 0.000 claims description 2
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- 239000000126 substance Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 abstract description 20
- 239000008280 blood Substances 0.000 abstract description 20
- 238000007789 sealing Methods 0.000 abstract description 5
- 230000035602 clotting Effects 0.000 abstract 2
- 230000005856 abnormality Effects 0.000 abstract 1
- 238000003018 immunoassay Methods 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
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- 239000012790 adhesive layer Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 102000009091 Amyloidogenic Proteins Human genes 0.000 description 3
- 108010048112 Amyloidogenic Proteins Proteins 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 2
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a novel crown detection micro-fluidic device based on coagulation dysfunction, which is used in the medical detection fields of immunoassay, instant detection and the like. The novel coronary patient clotting abnormality marker is amyloid in whole blood, and the novel coronary detection is realized by labeling the amyloid which can react with the clotting function by adopting a fluorescent probe. The microfluidic device is an instant detection chip and comprises a three-layer structure: cover plate, middle plate, base plate. Wherein the cover plate comprises a filtering area and a vent hole, which are respectively used for adding a blood sample and controlling the flowing state of fluid; the middle piece comprises a sample injection area through hole structure and is used for fixing filter cotton; the substrate comprises a filtering area, a mixing area, a delay area, a detection area and a waste liquid area; the three-layer structure forms a channel through sealing, so that the filtration of a whole blood sample, the full reaction of amyloid and fluorescent markers, the fluid flow control and the detection of amyloid are realized. The micro-fluidic device designed by the invention can realize the rapid and accurate detection of the novel coronary blood coagulation dysfunction, and has higher application value.
Description
Technical Field
The invention relates to a treatment fluid and a treatment method for designing a blood filtering pad and a sample pad attached with a fluorescent probe by using a microfluidic device for detecting novel coronary blood coagulation dysfunction, belonging to the research and development field of medical instant detection products.
Background
Blood has close relation with tissue and organs of various systems of the whole body, and blood detection is the most widely used sample detection in biomedicine, clinical diagnosis and health quarantine diagnosis. Currently, most hospitals and laboratories use large centrifuges to separate serum and blood cells, and then detect the levels of various indicators of blood in automated biochemical analyzer equipment. However, the method needs to draw a milliliter amount of blood sample from a patient, and the sample actually used for detection is only a few microliters, so that a large amount of sample is wasted, the centrifuge has a large volume and a complex structure, and cannot be directly connected with blood detection equipment, so that blood separation and blood detection need to be separately and independently carried out, sample pollution is easily caused, the detection efficiency is low, and the requirements of safe and rapid blood detection cannot be met. In addition, the automatic biochemical analyzer has huge volume, high price and complex operation, and the required sample amount is large, so that the automatic biochemical analyzer is not applicable to basic medical institutions, and is difficult to meet the requirements of on-site sampling analysis, rapid detection, self-test of patients and the like.
Therefore, development of a miniature portable whole blood detection technology and apparatus which are easy to operate and have a small sample size has become extremely urgent.
The microfluidic instant detection chip is a detection platform based on a microfluidic technology, and can realize high-sensitivity, high-speed and high-flux detection of samples. The micro-fluidic technology utilizes micro-manufacturing technologies such as micro channels, micro valves, micro pumps and the like to control and process samples under a micro scale, so that accurate control and control of micro fluid samples are realized. And detection of different samples can be adapted by structural optimization and control of reaction time.
Disclosure of Invention
Invention of the inventionProblems to be solved
In order to solve the problems of low detection efficiency, huge volume, high price, complex operation, large required sample amount and the like of a detection instrument in the prior art, and improve the reliability of whole blood detection under the condition of small sample amount, the invention designs a micro-fluidic detection chip which has small volume, low cost and convenient use, has low requirements on equipment complexity, and can realize high-precision and rapid detection of blood only by a small amount of samples.
Solution for solving the problem
The inventor has made an effort to solve the above problems and designed a microfluidic detection chip, and specifically, the invention includes the following technical scheme:
[1] a microfluidic detection chip comprising three stacked layers, a chip cover comprising a filtration zone and a vent; the middle chip is provided with a filtering area and a waste liquid area; the chip substrate is designed with a groove as a sample channel; the sample channel comprises a filtering area, a mixing area, a time delay area, a detection area and a waste liquid area, wherein the sample filtering area corresponds to the filtering area of the chip cover plate, and the three layers of chips are welded by ultrasonic stitching.
[2] The microfluidic detection chip according to any one of the above embodiments, wherein the chip cover sheet and the chip substrate are made of one of polystyrene, polydimethylsiloxane, polymethyl methacrylate, polyethylene terephthalate, and polycarbonate; preferably, the materials of the chip cover sheet and the chip substrate are selected from polymethyl methacrylate, polystyrene or polycarbonate; the middle layer of the chip is polyethylene terephthalate glue or polymethyl methacrylate glue.
[3] The microfluidic detection chip according to any one of the above technical solutions, wherein the groove corresponds to the lower half of the waste liquid tank region, and the depth is 1-2mm.
[4] According to the technical scheme of [1], the thickness of the chip cover plate and the thickness of the chip substrate are 1-2mm, and the thickness of the chip intermediate layer is 0.5-1.5mm.
Drawings
Fig. one is an external view of a first example of a microfluidic chip according to the present invention.
And a second diagram is a cover plate structure diagram of the first example of the microfluidic chip provided by the invention.
And third, the structure diagram of the middle piece of the first example of the microfluidic chip provided by the invention.
Fig. four is a substrate structure diagram of a first example of the microfluidic chip provided by the invention.
Description of the reference numerals
Chip cover 100
Chip middle sheet 200
Chip substrate 300
Sample addition port 101
Vent hole 102
Filtration zone 201
Waste liquid zone 202
Filtration zone 301
Mixing zone 302
Delay zone 303
Detection zone 304
Waste liquid zone 305
Description of the embodiments
To more clearly illustrate the above objects, features and advantages of the present invention, specific embodiments of the present invention are described in detail in this section with reference to the accompanying drawings. The present invention can be embodied in various forms other than those described in this section, and modifications, variations, and alternatives thereto can be made by those skilled in the art without departing from the spirit of the invention, and therefore the invention is not limited to the specific examples disclosed in this section. The protection scope of the present invention shall be subject to the claims.
The term "comprising" in the description of the invention and the claims and in the above figures and any variants thereof is intended to cover a non-exclusive inclusion. For example, a process, method, or system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed but may optionally include additional steps or elements not listed or inherent to such process, method, article, or apparatus.
In addition, numerous specific details are set forth in the following detailed description in order to provide a better understanding of the present disclosure. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In some instances, well known methods, procedures, components, and circuits have not been described in detail so as not to obscure the present invention.
< microfluidic detection chip >
The microfluidic detection chip comprises three stacked layers: chip cover plate, chip substrate, chip intermediate layer. The shape of the chip may be oval, square, rectangular, polygonal or circular, preferably it is a conventional rectangle for better handling, viewing and transportation. The thickness of the substrate on the chip is 1-2mm, if the thickness is too thin, the sample loading amount of the chip is too small and is easy to deform, and if the thickness is too thick, the light transmittance can be influenced, the detection result is influenced, and meanwhile, the chip miniaturization requirement is not met. The thickness of the middle layer of the chip is 0.5-1.5mm. The chip cover plate and the substrate are stuck by an intermediate layer or sewn and fixed by ultrasonic waves.
The material of the chip cover plate and the chip middle plate is selected from one of polystyrene, polydimethylsiloxane, polymethyl methacrylate, polyethylene terephthalate, glass or polycarbonate; preferably, the chip material is selected from polymethyl methacrylate, polystyrene or polycarbonate;
the chip cover is mainly used for introducing the sample into the detection area. The chip cover plate comprises a filtering area, a sample adding hole is arranged on the filtering area and used for adding samples, the sample adding hole can be designed into a round shape or a semicircular shape which can be closely matched with a standard pipette gun head of a routine experiment in a biological experiment, the diameter of the sample adding hole can be between 2mm and 3mm, and the samples added through the sample adding hole can flow along a sample flow channel. Because the invention adopts gravity and capillary action to self-drive, the whole blood filtering device such as a whole blood filter membrane and the like is fixed in the filtering area, so that a centrifugal device and the like are not needed to remove blood cells, the cost is saved, and the use efficiency is improved. The shape of the filtering area corresponds to the shape of the sample groove area of the middle layer of the chip.
The chip cover plate also comprises vent holes, namely a sample adding hole of the filtering area and a vent hole of the waste liquid pool, wherein the vent holes are through holes communicated with the atmosphere, and are preferably circular through holes which are uniformly arranged so as to improve the fluidity of sample solution, and the diameter can be in the range of 0.5-2.0 mm. In one possible embodiment, the chip cover plate is provided with 1 filtration zone loading well and 1 set of evenly arranged waste reservoir vents. In another possible embodiment, a plurality of filter zone vents may be provided, depending on the number of filter zones.
The chip intermediate layer is the glue film with have gluey district and no gluey district to separate the sample flow channel on the glue film, the sample flow channel includes filtration district, mixed district, time delay district, detection zone, waste liquid district, wherein filtration district with the filtration district of chip substrate corresponds, the shape of filtration district can be the same with the filtration district of chip substrate. When the chip is sealed with the substrate and the intermediate layer, the main function of the waste liquid tank area is to store waste liquid, namely the waste liquid tank. The chip intermediate layer and the substrate can also be provided with energy guide ribs, so that the sealing performance and stability of the three-layer structure in ultrasonic seaming are improved.
< use of microfluidic detection chip >
When the microfluidic detection chip is used, 100-300ul of fresh blood can be directly dripped into a sample adding port, a sample flows in a sample flow channel under the action of gravity and capillary action, blood cells are filtered out at the sample adding port under the action of filter cotton, a fluorescent probe is dissolved at the same time, the sample is fully mixed with the fluorescent probe under the action of a mixing region, then the sample is stopped in a time delay zone under the action of a time delay zone structure and air pressure, and the sealing state of the sample flow channel and the external environment is released after 10-15 minutes of fluorescent staining is fully completed, namely, a vent hole of a waste liquid region is opened. After the air pressure is communicated, the fluid slowly flows into the detection area through the delay area structure, thrombin factors pre-buried in the detection area are captured and settled in the detection area, at the moment, marked amyloid protein is settled in the detection area, and the rest of the fluid enters the waste liquid area for storage after passing through the area. And (5) reading the fluorescence intensity of the detection area through fluorescence detection to judge the index to be detected.
The invention is further illustrated, but not limited, by the following examples.
Examples
Examples
The embodiment provides a microfluidic detection chip for qualitatively detecting amyloid.
Fig. 1 is a diagram showing the outline of the microfluidic detection chip of the present embodiment. As can be seen from the figure, the microfluidic detection chip 1 is rectangular in shape, and has dimensions of 20mm×90mm×3mm in length, width and height. The chip comprises three stacked layers: chip cover 100, chip middle 200, chip substrate 300; the thickness of the three layers of chips is 1mm.
The chip cover 100 is made of polymethyl methacrylate, and the structure of the chip cover 100 is shown in fig. two, so that the chip cover 100 can be seen to comprise a filtering area 101, and a sample adding hole 102 is formed in the filtering area 101 and is used for adding a sample, and the radius of the sample adding hole 102 is 2.5mm. The filtering area structure is trapezoid. Chip cover 100 also includes vent holes of diameter 0.5mm, i.e., filtration area loading well 103 and waste well vent 104 as shown in FIG. 1, arranged in 3*5. The vent hole has a circular pupil identical to the atmosphere to improve the fluidity of the sample solution.
The material of the chip middle sheet 200 is polymethyl methacrylate, the shape of the chip middle sheet is matched with that of the chip substrate, and the thickness of the chip middle sheet 200 is 1mm. The middle chip sheet is a double-sided adhesive layer made of PET (polyethylene terephthalate) adhesive, and the structure of the middle chip layer 200 shown in the figure three is that a sample flow channel is separated on the double-sided adhesive layer by an adhesive area 301 and an adhesive-free area 302.
The chip substrate 300 is made of polyethylene terephthalate glue, a sample flow groove is processed on the chip, and the second diagram shows the chip substrate structure, which comprises a filtering area 301, a mixing area 302, a delay area 303, a detection area 304 and a waste liquid area 305; the filtering area 301 is provided with a trapezoid with filtering cotton of 12.9mm at the upper bottom, 10mm at the lower bottom and 20mm in height; the mixing area 302 is a cylindrical structure with uniformly distributed diameters and intervals of 50um; the delay area 303 is designed with a serpentine channel structure, which is characterized in that the channel has a height of 90um and a width of 300um, one end of the channel is connected with the lower side of the channel, and the other end extends to 500um from the upper side of the channel; the detection area 304 is designed with a microarray structure, wherein the microarray structure is a cylindrical groove structure with the diameter and the interval of 50um which are uniformly distributed, and thrombin factors are pre-embedded in the groove structure; the waste liquid area 305 is a rectangular groove with a length of 6mm, a width of 12mm and a depth of 100um.
When the detection chip is prepared, the adhesive coating on one side of the middle chip 300 is torn off and stuck on the chip substrate 200, and the filter cotton and the filter areas 201 and 301 are fixed, fluorescent probe particles are attached to the filter cotton, the cover plate of the chip cover plate is stuck to the filter cotton to be tightly pressed after the filter cotton is air-dried for half an hour, and the ultrasonic stitching is performed by using an ultrasonic stitching machine after the filter cotton is air-dried for half an hour.
In the detection process, the vent hole 104 is sealed, 200ul of whole blood is directly dripped into the sample adding hole 102, after waiting for 10 minutes, the sealing of the vent hole 104 is released, the sample reaction is finished, the sample enters the detection area 304 through the time delay area 303, after the thrombin factor precipitates the dyed amyloid protein, the rest of samples enter the waste liquid area 305 through the detection area, and then the qualitative judgment is carried out by detecting the fluorescence intensity in the detection area.
Examples
The embodiment provides a microfluidic detection chip for qualitatively detecting amyloid.
Fig. 1 shows an outline view of the microfluidic detection chip of the present embodiment. As can be seen from the figure, the microfluidic detection chip 1 is rectangular in shape, and has dimensions of 20mm×90mm×3mm in length, width and height. The chip comprises three stacked layers: chip cover 100, chip middle 200, chip substrate 300; the thickness of the three layers of chips is 1mm.
The chip cover 100 is made of polystyrene or polycarbonate, and the chip cover 100 shown in fig. 2 is structured, so that the chip cover 100 can be seen to comprise a filtering area 101, and a sample adding hole 102 is formed in the filtering area 101 for adding a sample, wherein the radius of the sample adding hole 102 is 2.5mm. The filtering area structure is trapezoid. Chip cover 100 also includes vent holes of diameter 0.5mm, i.e., filtration area loading well 103 and waste well vent 104 as shown in FIG. 1, arranged in 3*5. The vent hole has a circular pupil identical to the atmosphere to improve the fluidity of the sample solution.
The material of the chip middle sheet 200 is polystyrene or polycarbonate, the shape of the chip middle sheet is matched with that of the chip substrate, and the thickness of the chip middle sheet 200 is 1.5mm. The middle chip sheet is a double-sided adhesive layer made of PET (polyethylene terephthalate) adhesive, and the structure of the middle chip layer 200 shown in FIG. 3 is that a sample flow channel is separated on the double-sided adhesive layer by an adhesive area 301 and an adhesive-free area 302.
The chip substrate 300 is made of polymethyl methacrylate adhesive, and is provided with a sample flow groove, wherein the sample flow groove comprises a filtering area 301, a mixing area 302, a delay area 303, a detection area 304 and a waste liquid area 305; the filtering area 301 is provided with a trapezoid with filtering cotton of 12.9mm at the upper bottom, 10mm at the lower bottom and 20mm in height; the mixing area 302 is a cylindrical structure with uniformly distributed diameters and intervals of 50um; the delay area 303 is designed with a serpentine channel structure, which is characterized in that the channel has a height of 90um and a width of 200um, one end of the channel is connected with the lower side of the channel, and the other end extends to 250um from the upper side of the channel; the detection area 304 is designed with a microarray structure, wherein the microarray structure is a cylindrical groove structure with the diameter and the interval of 50um which are uniformly distributed, and thrombin factors are pre-embedded in the groove structure; the waste liquid area 305 is a rectangular groove with a length of 6mm, a width of 12mm and a depth of 100um.
When the detection chip is prepared, the adhesive coating on one side of the middle chip 300 is torn off and stuck on the chip substrate 200, and the filter cotton and the filter areas 201 and 301 are fixed, fluorescent probe particles are attached to the filter cotton, the cover plate of the chip cover plate is stuck to the filter cotton to be tightly pressed after the filter cotton is air-dried for half an hour, and the ultrasonic stitching is performed by using an ultrasonic stitching machine after the filter cotton is air-dried for half an hour.
In the detection process, the vent hole 104 is sealed, 300ul of whole blood is directly dripped into the sample adding hole 102, after waiting for 15 minutes, the sealing of the vent hole 104 is released, the sample reaction is finished, the sample enters the detection area 304 through the time delay area 303, after the thrombin factor precipitates the dyed amyloid protein, the rest of samples enter the waste liquid area 305 through the detection area, and then the qualitative judgment is carried out by detecting the fluorescence intensity in the detection area.
Claims (12)
1. The microfluidic device design for detecting the novel coronary blood coagulation dysfunction is characterized in that the microfluidic chip comprises a three-layer structure, and a chip cover plate comprises a filtering area and a vent hole; the chip substrate is designed with a groove as a fluid channel; the sample flow channel comprises a filtering area, a mixing area, a time delay area, a detection area and a waste liquid area.
2. The three-layer structure of claim 1 wherein the filtration zone of the substrate corresponds to the cover slip loading port and the substrate waste zone corresponds to the cover slip vent. The middle chip comprises a hollowed-out structure corresponding to the negative film filtering area and the waste liquid area.
3. The microfluidic chip according to claim 1 or 2, wherein the materials of the cover sheet, the intermediate sheet and the substrate of the microfluidic chip are selected from polystyrene, polydimethylsiloxane, polymethyl methacrylate, polyethylene terephthalate, glass or polycarbonate.
4. The microfluidic detection chip substrate according to claim 1, wherein: and S1, the depth of the groove serving as the fluid channel is 100um. S2, the microfluidic detection chip according to claim 1 is characterized in that the thicknesses of the chip cover plate and the substrate are 1-2mm, the thickness of the chip intermediate layer can be set to be generally 0.5-1.5mm according to the required sample loading amount, and the sizes of the chip cover plate, the intermediate plate and the substrate are 20 x 90mm. S3, the microfluidic detection chip according to claim 1 is characterized in that filter cotton attached with fluorescent probes is placed in a substrate filter area of the chip, and the filter cotton is simultaneously fixed by a hollowed-out structure of an intermediate layer. S4, the microfluidic detection chip according to claim 1 is characterized in that a microarray structure is designed in a mixing area of the chip substrate, and the microarray structure is a cylindrical structure with uniformly distributed diameters and pitches of 50um-100 um. S5. The microfluidic detection chip according to claim 1, wherein the delay area of the chip substrate is designed with a serpentine channel structure, and the chip substrate is characterized in that the channel has a height of 60-90um and a width of 200-300um, one end of the channel is connected with the lower side of the channel, and the other end extends to 450-500um from the upper side of the channel. The subsequent channels alternate with each other to form S-shaped channels to slow down the fluid passage. S6, the microfluidic detection chip according to claim 1 is characterized in that a microarray structure is designed in a detection area of the chip substrate, and the microarray structure is a cylindrical groove structure with uniformly distributed diameters and intervals of 50um-100 um. S7. The cylindrical well structure of microarray according to claim 8, wherein thrombin factor is attached to the well for the purpose of depositing amyloid flowing therethrough in the detection zone. S8, the microfluidic detection chip according to claim 1, wherein the waste liquid area of the chip substrate is designed into a rectangular groove with the length of 5-8mm, the width of 10-14mm and the depth of 90-120 um. The microfluidic detection chip according to claim 1, wherein the filter area of the middle chip has the same position and size as the filter area of the bottom chip, and is in a hollow structure; the purpose is to fix the filter cotton of the bottom plate. The microfluidic detection chip according to claim 1, wherein the waste liquid area of the middle chip has the same position and size as the waste liquid area of the bottom chip, and is in a hollow structure; the purpose is to create a pathway for the underlying microfluidic channel to communicate with the atmosphere.
5. The microfluidic chip according to claim 1, wherein the filter area of the chip cover plate has a semicircular structure with a radius of 2-3mm, is located at a central position and is 5-6mm from the top end of the chip, and is used as a sample loading port of the whole chip.
6. The microfluidic chip according to claim 1, wherein the waste liquid area of the chip cover plate is provided with uniformly arranged hole structures with the radius of 0.5-2mm and the same interval as the radius, which are used as vents between the microfluidic channel in the chip and the outside, and thus the opening and closing of the structures control the internal air pressure of the microfluidic channel.
7. The microfluidic detection chip according to claim 1, wherein the three-layer structure is tightly laminated by means of glue, ultrasonic welding or laser welding.
8. The microfluidic chip according to claim 1, wherein the microfluidic chip can add a sample to be tested through the filtering area, mix the sample to be tested with the pre-buried fluorescent probe through the mixing area and the delay area, control the reaction time, finally capture the detection reaction result of the labeled substance in the detection area, and collect the waste liquid in the waste liquid area.
9. The microfluidic chip according to claim 1, wherein the microfluidic chip can adapt to different detection requirements by optimizing the adjustment structure to control the sample flow rate and reaction time.
10. The microfluidic chip according to claim 1, wherein the microfluidic chip can rapidly detect coagulation abnormal conditions in a sample, and has the characteristics of high sensitivity and high precision.
11. The microfluidic detection chip according to claim 1, wherein the microfluidic chip has the advantages of simple manufacturing process, low cost, easy mass production and the like.
12. The microfluidic chip according to claim 1, wherein the microfluidic chip can be widely used in the fields of medical diagnosis, drug screening, life science research, and the like.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311568593.7A CN117619463A (en) | 2023-11-22 | 2023-11-22 | New crown detection micro-fluidic device based on coagulation dysfunction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311568593.7A CN117619463A (en) | 2023-11-22 | 2023-11-22 | New crown detection micro-fluidic device based on coagulation dysfunction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117619463A true CN117619463A (en) | 2024-03-01 |
Family
ID=90031436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311568593.7A Pending CN117619463A (en) | 2023-11-22 | 2023-11-22 | New crown detection micro-fluidic device based on coagulation dysfunction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117619463A (en) |
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2023
- 2023-11-22 CN CN202311568593.7A patent/CN117619463A/en active Pending
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