CN117617499B - 肉桂精油-植物多酚自组装复合乳液及其制备方法和应用 - Google Patents

肉桂精油-植物多酚自组装复合乳液及其制备方法和应用 Download PDF

Info

Publication number
CN117617499B
CN117617499B CN202311614433.1A CN202311614433A CN117617499B CN 117617499 B CN117617499 B CN 117617499B CN 202311614433 A CN202311614433 A CN 202311614433A CN 117617499 B CN117617499 B CN 117617499B
Authority
CN
China
Prior art keywords
essential oil
cinnamon essential
polyphenol
self
composite emulsion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202311614433.1A
Other languages
English (en)
Other versions
CN117617499A (zh
Inventor
陈绵鸿
李如一
程方滢
冯慧坤
何云侠
李家祺
涂浩
周伟
李积华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agricultural Products Processing Research Institute of CATAS
Original Assignee
Agricultural Products Processing Research Institute of CATAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agricultural Products Processing Research Institute of CATAS filed Critical Agricultural Products Processing Research Institute of CATAS
Priority to CN202311614433.1A priority Critical patent/CN117617499B/zh
Publication of CN117617499A publication Critical patent/CN117617499A/zh
Application granted granted Critical
Publication of CN117617499B publication Critical patent/CN117617499B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/015—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/03—Organic compounds
    • A23L29/035—Organic compounds containing oxygen as heteroatom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
    • A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/74—Rubiaceae (Madder family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/82—Theaceae (Tea family), e.g. camellia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A61K8/066—Multiple emulsions, e.g. water-in-oil-in-water
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A61K8/602—Glycosides, e.g. rutin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/113—Multiple emulsions, e.g. oil-in-water-in-oil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
    • A61K2800/30—Characterized by the absence of a particular group of ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Birds (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Dermatology (AREA)
  • Dispersion Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Molecular Biology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Inorganic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

本发明公开了一种肉桂精油‑植物多酚自组装复合乳液及其制备方法，该自组装复合乳液包括如下组分：肉桂精油、植物多酚和无水乙醇；植物多酚溶于磷酸缓冲液浓度优选为1‑40mg/mL，肉桂精油与植物多酚的质量比优选为0.25‑2.5，肉桂精油与无水乙醇的比例优选为0.5‑2(v/v)；该肉桂精油‑植物多酚自组装复合乳液及其制备方法通过超声触发油相肉桂精油和水相多酚溶液油水界面不平衡，从而产生趋向于均相平衡状态的驱动力诱导精油组分和多酚发生非共价相互作用，从而形成自组装复合乳液，而无需任何表面活性剂，形成的自组装复合乳液具有较好稳定性的同时还具备增强单体抗氧化和抗菌活性的作用。

Description

肉桂精油-植物多酚自组装复合乳液及其制备方法和应用
技术领域
本发明属于食品乳液技术领域，具体涉及一种肉桂精油-植物多酚自组装复合乳液及其制备方法和应用。
背景技术
仿生小分子自组装是自然界的一种普遍现象，开发小分子自组装体已被应用于生物医学领域。例如，分子自组装策略已被应用于生产许多结构良好的组装体，应用于医学、生物学、材料、药物递送等领域。目前，π-π堆积和氢键相互作用在分子自组装和药物传递系统中得到了广泛的应用。然而，疏水性肉桂精油与亲水性多酚生物活性小分子的自组装现象尚未见报道。
发明内容
为了克服现有技术的不足，本发明的目的在于提供一种通过超声触发油相肉桂精油和水相多酚溶液油水界面不平衡，从而产生趋向于均相平衡状态的驱动力诱导精油组分和多酚发生非共价相互作用，形成自组装复合乳液，无需任何表面活性剂的肉桂精油-植物多酚自组装复合乳液及其制备方法和应用。
本发明的目的通过以下技术方案实现：
一种肉桂精油-植物多酚自组装复合乳液，包括以下组分：肉桂精油、植物多酚、无水乙醇和磷酸缓冲液，其中，肉桂精油与无水乙醇混合溶解，植物多酚溶于磷酸缓冲液。
作为优选，肉桂精油与植物多酚的质量比为0.25-2.5。
作为优选，植物多酚溶于磷酸缓冲液浓度为1-40mg/mL。
作为优选，肉桂精油与无水乙醇的比例为0.5-2v/v。
作为优选，植物多酚为咖啡多酚、茶多酚或柚皮苷中的一种或多种。
作为优选，磷酸盐缓冲液的pH为4-10。
本发明所要解决的另一技术问题为提供一种肉桂精油-植物多酚自组装复合乳液的制备方法，包括以下步骤：
将肉桂精油与无水乙醇混匀溶解，得肉桂精油溶液；
将植物多酚溶于磷酸缓冲液，得多酚溶液；
在磁力搅拌下将肉桂精油溶液滴加到多酚溶液中混匀，得混合溶液；
将混合溶液在超声波清洗器和超声波破碎仪下进行超声处理；获得肉桂精油-植物多酚自组装复合乳液。
作为优选，超声波清洗器超声功率为100-900W，超声时间为5-60min。
作为优选，超声波破碎仪的超声功率为50-800W，超声时间为2-30min。
肉桂精油通常含有70％以上的肉桂醛，具有抗发炎、镇静麻醉、血管扩张，以及指痉挛的作用，对中枢神经有振奋与镇静双重作用。同时肉桂精油还是用于治疗多种感染的最强大的精油之一。但由于其疏水性强，在温度、湿度、pH、光照、氧气等复杂的外界环境下易降解，导致其生物利用度低，极大地阻碍了其在食品和化妆品领域的应用。
多酚是一种广泛存在于天然植物中的常见化合物，其分子结构中含有酚羟基，具有抗氧化、抗炎、抗菌和伤口治疗等作用。肉桂精油的主要成分肉桂醛可以通过π-π堆积、氢键、静电相互作用等作用方式与多酚发生相互自组装反应。
本发明通过超声触发油相肉桂精油和水相多酚溶液油水界面不平衡，从而产生趋向于均相平衡状态的驱动力诱导精油组分和多酚发生非共价相互作用，从而形成自组装复合乳液，而无需任何表面活性剂。
本发明具有如下有益效果：
通过超声触发油相肉桂精油和水相多酚溶液油水界面不平衡，从而产生趋向于均相平衡状态的驱动力诱导精油组分和多酚发生非共价相互作用(π-π堆积和氢键)，从而形成自组装复合乳液，而无需任何表面活性剂。形成的自组装复合乳液具有较好稳定性的同时还具备增强单体抗氧化和抗菌活性的作用。
附图说明
图1为实施例1～3所制得的肉桂精油-植物多酚自组装复合乳液的外观形貌图；
图2为不同咖啡多酚浓度复合乳液的微观结构图；
图3为不同咖啡多酚浓度复合乳液的粒径分布图；
图4为不同咖啡多酚浓度复合乳液的粒径图；
图5为不同咖啡多酚浓度复合乳液的PDI图；
图6为不同咖啡多酚浓度复合乳液的zeta电位图；
图7为不同咖啡多酚浓度复合乳液的离心稳定性图。
图8为肉桂精油和多酚中主要成分自组装形成复合乳液示意图；
图9为肉桂精油、咖啡多酚及其自组装复合乳液的紫外光谱图；
图10为肉桂精油、咖啡多酚及其自组装复合乳液的红外光谱图；
图11为肉桂精油、咖啡多酚及其自组装复合乳液的DSC曲线图；
图12为肉桂精油、咖啡多酚和肉桂精油-咖啡多酚复合乳液的不同稀释倍数下的总抗氧化能力图；
图13为肉桂精油、咖啡多酚和肉桂精油-咖啡多酚复合乳液的对金黄色葡萄球菌和大肠杆菌的抗菌能力图。
具体实施方式
本发明的实施方式不限于此，按照本发明的上述内容，利用本领域的普通技术知识和惯用手段，在不脱离本发明上述基本技术思想前提下，本发明还可以做出其它多种形式的修改、替换或组合，均落在本发明权利保护范围之内。
实施例一
一种肉桂精油-植物多酚自组装复合乳液的制备方法，包括以下步骤：
(1)精确称取肉桂精油200mg，与200μL无水乙醇混匀；
(2)精确称取咖啡多酚200mg，溶于pH7磷酸盐缓冲液(10mL)中；
(3)在磁力搅拌下将肉桂精油乙醇溶液滴加到咖啡多酚溶液中混匀；
(4)将肉桂精油-咖啡多酚混合溶液在超声波破碎仪下进行超声处理获得肉桂精油-咖啡多酚自组装复合乳液，超声条件为600W，超声时间10分钟，超声开5秒，超声关10秒。
实施例二
一种肉桂精油-植物多酚自组装复合乳液的制备方法，包括以下步骤：
(1)精确称取肉桂精油150mg，与200μL无水乙醇混匀；
(2)精确称取茶多酚250mg，溶于pH8磷酸盐缓冲液(10mL)中；
(3)在磁力搅拌下将肉桂精油乙醇溶液滴加到茶多酚溶液中混匀；
(4)将肉桂精油-茶多酚混合溶液在超声波清洗器下进行超声处理获得肉桂精油-咖啡多酚自组装复合乳液，超声条件为500W，超声时间30分钟。
实施例三
(1)精确称取肉桂精油50mg，与75μL无水乙醇混匀；
(2)精确称取柚皮苷100mg，溶于pH10磷酸盐缓冲液(10mL)中；
(3)在磁力搅拌下将肉桂精油乙醇溶液滴加到柚皮苷溶液中混匀；
(4)将肉桂精油-柚皮苷混合溶液在超声波破碎仪下进行超声处理获得肉桂精油-柚皮苷自组装复合乳液，超声条件为800W，超声时间8分钟，超声开6秒，超声关10秒。
上述实施例1-3所制得的肉桂精油-植物多酚自组装复合乳液的外观形貌如图1所示。
图2为实施例1中制得的肉桂精油-植物多酚自组装复合乳液拍摄的微观结构，从图2中可以观察到，复合乳液中随着咖啡多酚的浓度增加，相同浓度的肉桂精油(20mg/mL)在咖啡多酚溶液中其油滴越分散，且其微观结构为球体。
从图3-6中可以看到，当固定肉桂精油浓度(20mg/mL)时，随着咖啡多酚浓度的增加，乳液的平均粒径越小，结果与显微镜观察到结果一致。同时，含咖啡多酚的复合乳液的PDI值小于0.3，表明所制备的复合乳液均一性比纯精油乳液更好。Zeta电位结果显示咖啡多酚的加入增加了油滴表明的负电荷，表明咖啡多酚和肉桂精油存在静电相互作用。通常zeta电位绝对值越大，体系越稳定，CP20为实施例一。
如图7所示，通过稳定性分析仪测定了不同咖啡多酚浓度复合乳液的离心稳定性，结果表明随着咖啡多酚浓度的增加，复合乳液的稳定性逐渐增加，不稳定性指数逐渐下降，这与复合乳液中乳滴粒径较小且均一，zeta电位值绝对值更大相关。综上所述，肉桂精油和咖啡多酚能经超声处理后自组装形成复合乳液，且咖啡多酚还能起到稳定和分散肉桂精油乳滴的作用，CP20为实施例一。
如图8-11所示，肉桂精油中主要成分肉桂醛可以通过π-π堆积、氢键等与多酚中的酚羟基进行自组装形成复合乳液。图9中复合乳液(EM，实施例一)的紫外扫描光谱图的特征吸收峰与肉桂精油(EO)一致，但是相同浓度下吸光值大于EO，这是由于咖啡多酚(CP)也存在吸收峰叠加到一起，表明该复合乳液中存在肉桂精油和咖啡多酚的特征吸收，二者存在一定的非共价相互作用。图10展示了冻干后的咖啡多酚和肉桂精油-咖啡多酚复合乳液(实施例一)的红外光谱图，乳液在1666cm-1出现了肉桂醛的羰基特征吸收峰，表明肉桂精油和咖啡多酚结合良好，在冻干后仍保存在咖啡多酚粉末中，二者之间存在较强的相互作用力。图11中显示肉桂精油、咖啡多酚及其复合乳液(实施例一)的热吸收峰温度，复合乳液与两种单体吸收峰温度均不同，这是因为咖啡多酚和肉桂精油之间形成了新的分子内和分子间氢键，表明肉桂精油与咖啡多酚之间并非简单的混合叠加而是通过发生相互作用形成复合乳液。
如图12-13所示，考察了肉桂精油、咖啡多酚两种单体以及二者自组装复合乳液(实施例一)的抗氧化能力和抗菌能力。结果显示，与单体肉桂精油和咖啡多酚相比，复合乳液的抗氧化能力和抗菌能力均有所提高，抗氧化能力和抗菌能力强于同浓度下的单体肉桂精油和咖啡多酚。结果表明二者的复合起到协同增效的作用，在食品、化妆品和生物医学具有较大的应用潜力。
说明：以上实施例仅用以说明本发明而并非限制本发明所描述的技术方案；因此，尽管本说明书参照上述的各个实施例对本发明已进行了详细的说明，但是本领域的普通技术人员应当理解，仍然可以对本发明进行修改或等同替换；而一切不脱离本发明的精神和范围的技术方案及其改进，其均应涵盖在本发明的权利要求范围内。

Claims (6)

1.一种肉桂精油-咖啡多酚自组装复合乳液，其特征在于，包括以下组分：肉桂精油、植物多酚、无水乙醇和磷酸缓冲液，其中，肉桂精油与无水乙醇混合溶解，植物多酚溶于磷酸缓冲液；肉桂精油与植物多酚的质量比为0.25-2.5；该肉桂精油-咖啡多酚自组装复合乳液的制备方法，包括以下步骤：
将肉桂精油与无水乙醇混匀溶解，得肉桂精油溶液；
将植物多酚溶于磷酸缓冲液，得多酚溶液；
在磁力搅拌下将肉桂精油溶液滴加到多酚溶液中混匀，得混合溶液；
将混合溶液在超声波清洗器或超声波破碎仪下进行超声处理；获得肉桂精油-咖啡多酚自组装复合乳液；
该植物多酚为咖啡多酚。
2.根据权利要求1所述的肉桂精油-咖啡多酚自组装复合乳液，其特征在于：植物多酚溶于磷酸缓冲液浓度为1-40 mg/mL。
3.根据权利要求1所述的肉桂精油-咖啡多酚自组装复合乳液，其特征在于：肉桂精油与无水乙醇的比例为0.5-2v/v。
4.根据权利要求1或2所述的肉桂精油-咖啡多酚自组装复合乳液，其特征在于：磷酸盐缓冲液的pH为 4- 10。
5.根据权利要求1所述的肉桂精油-咖啡多酚自组装复合乳液，其特征在于：超声波清洗器超声功率为100-900W，超声时间为5-60min。
6.根据权利要求1或5所述的肉桂精油-咖啡多酚自组装复合乳液，其特征在于：超声波破碎仪的超声功率为50-800W，超声时间为2-30min。
CN202311614433.1A 2023-11-29 2023-11-29 肉桂精油-植物多酚自组装复合乳液及其制备方法和应用 Active CN117617499B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311614433.1A CN117617499B (zh) 2023-11-29 2023-11-29 肉桂精油-植物多酚自组装复合乳液及其制备方法和应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311614433.1A CN117617499B (zh) 2023-11-29 2023-11-29 肉桂精油-植物多酚自组装复合乳液及其制备方法和应用

Publications (2)

Publication Number Publication Date
CN117617499A CN117617499A (zh) 2024-03-01
CN117617499B true CN117617499B (zh) 2024-08-06

Family

ID=90035231

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202311614433.1A Active CN117617499B (zh) 2023-11-29 2023-11-29 肉桂精油-植物多酚自组装复合乳液及其制备方法和应用

Country Status (1)

Country Link
CN (1) CN117617499B (zh)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106038327A (zh) * 2016-07-04 2016-10-26 江苏大学 一种酪蛋白‑肉桂精油脂质体抗菌剂的制备方法和用途
CN113521004A (zh) * 2021-06-24 2021-10-22 中国农业科学院农产品加工研究所 一种高负载大麻二酚水包油大分子颗粒乳液的制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2715582B1 (fr) * 1994-02-02 1996-03-15 Centre Nat Rech Scient Microcapsules à paroi de flavonoïde réticulée et compositions en contenant.
CN116850142A (zh) * 2023-05-12 2023-10-10 吉林大学 一种丁香酚Pickering乳液及其制备方法与应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106038327A (zh) * 2016-07-04 2016-10-26 江苏大学 一种酪蛋白‑肉桂精油脂质体抗菌剂的制备方法和用途
CN113521004A (zh) * 2021-06-24 2021-10-22 中国农业科学院农产品加工研究所 一种高负载大麻二酚水包油大分子颗粒乳液的制备方法

Also Published As

Publication number Publication date
CN117617499A (zh) 2024-03-01

Similar Documents

Publication Publication Date Title
Sang et al. Comparison of three water-soluble polyphosphate tripolyphosphate, phytic acid, and sodium hexametaphosphate as crosslinking agents in chitosan nanoparticle formulation
Horuz et al. Nanoencapsulation of carotenoids extracted from tomato peels into zein fibers by electrospinning
Ahmad et al. Quantification and brain targeting of eugenol-loaded surface modified nanoparticles through intranasal route in the treatment of cerebral ischemia
Sonaje et al. Development of biodegradable nanoparticles for oral delivery of ellagic acid and evaluation of their antioxidant efficacy against cyclosporine A-induced nephrotoxicity in rats
AU2017208307B2 (en) Therapeutic polymeric nanoparticles and methods of making and using same
Jelvehgari et al. Development of pH-sensitive insulin nanoparticles using Eudragit L100-55 and chitosan with different molecular weights
Lakshmana Prabu et al. Formulation and evaluation of sustained release microspheres of rosin containing aceclofenac
Raja et al. Development and evaluation of curcumin encapsulated self-assembled nanoparticles as potential remedial treatment for PCOS in a female rat model
Pagar et al. Rivastigmine-loaded L-lactide-depsipeptide polymeric nanoparticles: decisive formulation variable optimization
Kumar et al. In vitro physicochemical characterization and short term in vivo tolerability study of ethionamide loaded PLGA nanoparticles: potentially effective agent for multidrug resistant tuberculosis
Paulo et al. Microencapsulation of caffeic acid and its release using aw/o/w double emulsion method: Assessment of formulation parameters
JP2020079249A (ja) 注射のためのテコビリマットの医薬組成物およびその調製方法
Contado et al. Influence of secondary preparative parameters and aging effects on PLGA particle size distribution: a sedimentation field flow fractionation investigation
Manne et al. Pterocarpus marsupium Roxb. heartwood extract synthesized chitosan nanoparticles and its biomedical applications
Feng et al. The selective encapsulation and stabilization of cinnamaldehyde and eugenol in high internal phase Pickering emulsions: Regulating the interfacial properties
Jiang et al. Chitosan nanoparticles loaded with Eucommia ulmoides seed essential oil: Preparation, characterization, antioxidant and antibacterial properties
KR20190010212A (ko) 레스베라트롤의 나노캡슐 제조방법 및 제조된 레스베라트롤 나노캡슐
Tong et al. Microenvironment-Responsive antibacterial, Anti-Inflammatory, and antioxidant Pickering emulsion stabilized by Curcumin-Loaded tea polyphenol particles for accelerating infected wound healing
Begum et al. Formulation and evaluation of dasatinib loaded solid lipid nanoparticles
Li et al. Electrospun quercetin-loaded zein nanoribbons
Xing et al. Chitosan/alginate nanoparticles stabilized by poloxamer for the controlled release of 5‐fluorouracil
Shi et al. Topical gel based nanoparticles for the controlled release of oleanolic acid: design and in vivo characterization of a cubic liquid crystalline anti-inflammatory drug
CN117617499B (zh) 肉桂精油-植物多酚自组装复合乳液及其制备方法和应用
Javadi et al. Electrospun PEO/WPI nanofibers with vanillin for food applications
CN114642652B (zh) 一种岩藻多糖自组装载药纳米粒制备及应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant