CN117603810A - Protein peptide extraction and purification system for enhancing immune function - Google Patents
Protein peptide extraction and purification system for enhancing immune function Download PDFInfo
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
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- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
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- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
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- C07K1/14—Extraction; Separation; Purification
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- C07K1/14—Extraction; Separation; Purification
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- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
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- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention relates to the technical field of protein peptide production, and provides a protein peptide extraction and purification system capable of enhancing immune function, which comprises a server, raw materials, a processing tank, a raw material pretreatment module, a protein peptide extraction module, a quality control module, a purification module and an evaluation module, wherein the raw material pretreatment module is used for pretreating the raw materials to form pretreatment liquid, the protein peptide extraction module is used for extracting target protein peptides after pretreatment to form extraction liquid, the quality control module is used for collecting a protein peptide extraction process, analyzing the collected state data to form an analysis result and dynamically adjusting the extraction process, the purification module is used for purifying the extraction liquid, the evaluation module is used for collecting purification data of the purification process of the purification module, evaluating the purification process to form an evaluation result according to the purification data, and triggering the purification module to adjust the purification process according to the evaluation result.
Description
Technical Field
The invention relates to the technical field of protein peptide production, in particular to a protein peptide extraction and purification system for enhancing immune function.
Background
With the development of biotechnology, specific protein peptides have shown important application values in the fields of medicine and bioscience, especially in enhancing immune functions. The protein peptide is one of the basic elements constituting organisms, and has various biological activities including antiviral, antibacterial, antitumor and the like. In immunology, specific protein peptides may be used as part of a vaccine or directly as immunomodulators.
For example, the prior art of chinese patent CN116392879B discloses a separation and purification device and purification method for collagen peptide, which solves the problems that the filter screen is easy to be blocked by impurities and is convenient to clean, but cannot realize efficient purification or purification.
Currently, common methods for extracting protein peptides from natural sources include chemical extraction, enzymatic extraction, microbial fermentation, and the like. However, these methods generally have the following problems:
1) The efficiency is low: many conventional methods are not effective in isolating and extracting the target protein peptide, resulting in low yields.
2) The selectivity is poor: it is difficult to precisely select and extract protein peptides having specific functions, often accompanied by a large amount of nonspecific proteins.
3) The product quality is unstable: due to the difference in extraction conditions, there may be fluctuations in the activity and purity of the product.
4) Loss of protein peptide activity during purification: during purification, the protein peptide may lose its biological activity due to unsuitable environmental conditions (e.g., pH, temperature).
The invention is designed to solve the problems that the purification efficiency is low, specific protein peptide cannot be identified, the extraction stability is poor, the quality cannot be evaluated, the activity of the purified protein peptide is lost, the purification stability is poor and the like in the field.
Disclosure of Invention
The invention aims at providing a protein peptide extraction and purification system for enhancing immune function, aiming at the defects existing at present.
In order to overcome the defects in the prior art, the invention adopts the following technical scheme:
a protein peptide extraction and purification system for enhancing immune function, the protein peptide extraction and purification system comprising a server, a raw material, and a processing tank, the protein peptide extraction and purification system further comprising a raw material pretreatment module, a protein peptide extraction module, a quality control module, a purification module, and an evaluation module, the server being configured to store intermediate data and process data of the raw material pretreatment module, the protein peptide extraction module, the quality control module, the purification module, and the evaluation module, the raw material pretreatment module being disposed in the processing tank and pre-treating the raw material;
The raw material pretreatment module is used for pretreating raw materials to form pretreatment liquid, the protein peptide extraction module is used for extracting target protein peptide of the pretreatment liquid to form extraction liquid, the quality control module is used for collecting target protein peptide extraction process, analyzing the collected state data to form analysis result and dynamically adjusting the extraction process, the purification module is used for purifying the extraction liquid, the evaluation module is used for collecting the purification data of the purification process of the purification module, evaluating the purification process according to the purification data to form evaluation result and triggering the purification module to adjust the purification process according to the evaluation result;
the protein peptide extraction module comprises an extraction tank, an ultrasonic auxiliary extraction unit and a selective adsorption unit, wherein the ultrasonic auxiliary extraction unit and the selective adsorption unit are arranged in the extraction tank, the ultrasonic auxiliary extraction unit is used for carrying out auxiliary release on protein peptide in the pretreated raw material, and the selective adsorption unit selectively adsorbs target protein peptide;
the selective adsorption unit comprises an adsorption column, an adsorption material, a sample injection member, a temperature control member and an elution member, wherein the adsorption column is filled with the adsorption material, the sample injection member inputs the pretreatment liquid containing the target protein peptide into the adsorption column, the temperature control member maintains the temperature in the adsorption column, and the elution member is used for eluting the target protein peptide from the adsorption material after adsorption.
Optionally, the sample injection member includes a transfer pump, a control valve, a transfer pipeline, and a flow meter, where the transfer pump provides a flow rate and a pressure to ensure transfer of the pretreatment liquid, one end of the transfer pipeline is connected to the extraction tank, the other end of the transfer pipeline extends to the adsorption column to supply the pretreatment liquid to the adsorption column, the flow meter is used to detect a flow rate in the transfer pipeline, and the control valve performs on-off control on the transfer pipeline;
the conveying pump is arranged in the conveying pipeline and conveys the pretreatment liquid through the conveying pipeline.
Optionally, the quality control module comprises a detection unit and an analysis unit, wherein the detection unit acquires state data of the target protein peptide in the adsorption column, and the analysis unit analyzes the target protein peptide according to the state data acquired by the detection unit;
wherein the status data comprises spectral characteristics of the target protein peptide.
Optionally, the analysis unit obtains the spectral characteristics of the target protein peptide and calculates the Purity index Purity of the target protein peptide according to the following formula:
；
Wherein A is 280 A is the absorption intensity value of the extracting solution at the spectral wavelength of 280nm 260 For the absorption intensity value of the extracting solution at the spectral wavelength of 260nm, CF is a correction factor, the value of which is an empirical value, and is determined according to the purified target protein historical data;
and if the Purity index Purity of the target protein peptide is lower than a monitoring threshold Range set by a system, adjusting the sample injection speed of the sample injection member and the temperature of the temperature control member.
Optionally, the purification module comprises a purification unit and a concentration unit, the purification unit further purifies the extracting solution to form primary treatment solution, and the concentration unit is used for removing small molecular impurities in the primary treatment solution so as to adjust the concentration of the primary treatment solution to form purified solution;
the purification unit includes a chromatographic column, a supply member injecting an extraction liquid into the chromatographic column, and purifying the extraction liquid through the chromatographic column, and a packing or medium filled in the chromatographic column.
Optionally, the evaluation module includes a sampling unit and an evaluation unit, the sampling unit collects purification data of the purification process of the purification unit, and the evaluation unit evaluates the purification process according to the purification data collected by the sampling unit;
The sampling unit comprises a first ultraviolet-spectrum detector, a PH sensor, a conductivity sensor and a data storage, wherein the first ultraviolet-spectrum detector is used for collecting ultraviolet absorption value data of the purified extracting solution, the PH sensor is used for collecting PH value data of the purified extracting solution, and the conductivity sensor is used for collecting the obtained purified extracting solution conductivity data; the data memory stores the ultraviolet absorption value data, the PH value data and the extracting solution conductivity data.
Optionally, the temperature control member includes a heater, a cooler and a cooler temperature sensor, the heater and the cooler are arranged on the side wall of the adsorption column, and the heater and the cooler are distributed in a staggered manner;
the cooler temperature sensor is arranged on the inner wall of the cooler and collects temperature data in the cooler.
Optionally, the eluting component includes a liquid storage tank, a spray head, a supply pump and an eluting pipeline, wherein the liquid storage tank stores the eluent, the supply pump is arranged at one end of the eluting pipeline to form a supply part, the supply part is arranged in the liquid storage tank and supplies the eluent in the liquid storage tank, and the spray head is arranged at the other end of the eluting pipeline and sprays the eluent on an adsorption column so as to flush the adsorbed target protein peptide.
Optionally, the evaluation unit acquires the purified data acquired by the sampling unit, and calculates the state index ACT according to the following formula:
；
in the UV norm Is the standardized value of the ultraviolet absorption value, pH norm Cond for normalization of pH norm Is the normalized value of conductivity, w 1 Is the weight coefficient of the ultraviolet absorption value, the value of the weight coefficient is set by a system, and w 2 Is a pH value weight coefficient, the value of which is set by a system, w 3 The value of the weight coefficient is set by the system.
Optionally, the adsorption material comprises ion exchange resin, affinity chromatography medium, and molecular imprinting polymer.
The beneficial effects obtained by the invention are as follows:
1. through the mutual coordination between the quality control module and the protein peptide extraction module, the extraction efficiency and the recognition capability of the target protein peptide are improved, and the whole system is ensured to have the advantages of high extraction stability, high extraction quality and high activity in the extraction process;
2. through the mutual matching of the ultrasonic auxiliary extraction unit and the selective adsorption unit, the protein peptide in the raw material can be fully released, the utilization rate of the raw material is improved, and the whole system has the advantage of high raw material utilization rate;
3. Through the mutual coordination between the stirring component and the enzyme releasing component, the reaction efficiency of the biological enzyme and the raw material can be accelerated in the stirring process, and the efficiency of releasing the cell content containing the target protein peptide from the raw material can be accelerated;
4. through the mutual coordination of the purification module and the protein peptide extraction module, the target protein peptide can be purified, the purification efficiency and the purification precision of the target protein peptide are improved, and the whole system has the advantages of accurate protein peptide identification, high extraction stability and good purification stability;
5. through the mutual cooperation of the purification module and the evaluation module, the evaluation of the purification process is more accurate and reliable, and the whole system is ensured to have the advantages of accurate quality evaluation, high protein peptide purification efficiency and high protein peptide stability.
Drawings
The invention will be further understood from the following description taken in conjunction with the accompanying drawings. The components in the figures are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the embodiments. Like reference numerals designate like parts in the different views.
Fig. 1 is a schematic block diagram of the overall structure of the present invention.
Fig. 2 is a block schematic diagram of an ultrasonic-assisted extraction unit, the selective adsorption unit and a purification unit of the present invention.
FIG. 3 is a schematic block diagram of the selective adsorption unit of the present invention for extracting target protein peptides.
Fig. 4 is a schematic diagram of an analysis flow of the detection unit and the analysis unit of the present invention.
FIG. 5 is a schematic diagram of the evaluation flow of the purification unit, concentration unit, sampling unit and evaluation unit of the present invention.
FIG. 6 is a schematic partial cross-sectional view of a feedstock pretreatment module of the present invention.
FIG. 7 is a schematic diagram showing the structure of a raw material pretreatment module and a protein peptide extraction module according to the present invention.
Fig. 8 is an enlarged schematic view at a in fig. 7.
Fig. 9 is an enlarged schematic view at B in fig. 7.
FIG. 10 is a schematic cross-sectional view of an adsorption column of the present invention.
Fig. 11 is an enlarged schematic view at C in fig. 10.
FIG. 12 is a schematic top view of the treatment tank, elution tank, and rotating member of the present invention.
FIG. 13 is a schematic partial cross-sectional view of a purification unit and a concentration unit of the present invention.
Reference numerals illustrate: 1. a treatment tank; 2. a heating member; 3. a supply conduit; 4. placing a tank; 5. a first storage tank; 6. a stirring driving mechanism; 7. a stirring rod; 8. a telescopic rod; 9. an interlayer; 10. a connecting pipe; 11. a second storage tank; 12. a first electronically controlled valve; 13. an extraction tank; 14. a lifting rod; 15. an ultrasonic generator; 16. a transmission pipeline; 17. a control valve; 18. electronically opening the valve; 19. an adsorption column; 20. a lifting rod; 21. a switching drive mechanism; 22. a switching lever; 23. an elution tank; 24. a first channel; 25. a second channel; 26. a permeable membrane; 27. a nozzle; 28. a temperature control member; 29. an adsorption material; 30. a spectrum detector; 31. a supply pipe; 32. an electronic supply valve; 33. a purification tank; 34. a chromatographic column; 35. a first ultraviolet-spectral detector; 36. a semipermeable membrane; 37. a filtering membrane; 38. a second ultraviolet spectrum detector; 39. a centrifuge tube temperature sensor; 40. a flow rate meter; 41. a centrifugal driving mechanism; 42. a drawing-out table; 43. centrifuge tube.
Detailed Description
The following embodiments of the present invention are described in terms of specific examples, and those skilled in the art will appreciate the advantages and effects of the present invention from the disclosure herein. The invention is capable of other and different embodiments and its several details are capable of modification and variation in various respects, all without departing from the spirit of the present invention. The drawings of the present invention are merely schematic illustrations, and are not intended to be drawn to actual dimensions. The following embodiments will further illustrate the related art content of the present invention in detail, but the disclosure is not intended to limit the scope of the present invention.
Embodiment one: according to fig. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 13, the present embodiment provides a protein peptide extraction and purification system for enhancing immune function, the protein peptide extraction and purification system including a server, a raw material, and a processing tank 1, the protein peptide extraction and purification system further including a raw material pretreatment module, a protein peptide extraction module, a quality control module, a purification module, and an evaluation module, the server being connected to the raw material pretreatment module, the protein peptide extraction module, the quality control module, the purification module, and the evaluation module, respectively, and storing intermediate data and process data of the raw material pretreatment module, the protein peptide extraction module, the quality control module, the purification module, and the evaluation module in a database of the server, the raw material pretreatment module being provided in the processing tank 1 and pretreating the raw material;
In addition, the raw materials include, but are not limited to, the following list of several: deep sea fish or specific plant seeds, milk, dairy products, microbial ferments, etc.;
the raw material pretreatment module is used for pretreating raw materials to form pretreatment liquid, the protein peptide extraction module is used for extracting target protein peptide of the pretreatment liquid to form extraction liquid, the quality control module is used for collecting a protein peptide extraction process, analyzing the collected state data to form an analysis result and dynamically adjusting the extraction process, the purification module is used for purifying the extraction liquid, the evaluation module is used for collecting purification data of the purification process of the purification module, evaluating the purification process according to the purification data to form an evaluation result and triggering the purification module to adjust the purification process according to the evaluation result;
the protein peptide extraction and purification system further comprises a central processor, wherein the central processor is respectively in control connection with the raw material pretreatment module, the protein peptide extraction module, the quality control module, the purification module and the evaluation module, and the raw material pretreatment module, the protein peptide extraction module, the quality control module, the purification module and the evaluation module are controlled in a centralized manner based on the central processor so as to improve the precision and the efficiency of the whole system for extracting and purifying the protein peptide;
The raw material pretreatment module comprises a biological tissue separation unit and an active ingredient stabilization unit, wherein the biological tissue separation unit is used for processing the raw materials to release cell contents containing target protein peptides, and the active ingredient stabilization unit is used for controlling the temperature, the PH value and additives in the pretreatment process to prevent the protein peptides from being denatured or inactivated;
the biological tissue separation unit includes an enzyme releasing member that releases biological enzymes, and a stirring member that stirs raw materials in the treatment tank 1 to promote the raw materials to release cellular contents containing target protein peptides;
the enzyme release member comprises a placing tank 4, a supply pump and a supply pipeline 3, wherein the placing tank 4 is used for storing biological enzymes, the supply pump is used for throwing the biological enzymes in the placing tank 4 into the treatment tank 1, one end of the supply pipeline 3 is connected with the supply pump, and the other end of the supply pipeline 3 extends into the treatment tank 1 so as to throw or supply the biological enzymes into the treatment tank 1;
in this embodiment, in the use process of treating the protein peptide, the protease of the type corresponding to the target protein peptide is used to decompose the biological tissue, so as to release the protein peptide, which is a technical means well known to those skilled in the art, and thus will not be described in detail herein;
The stirring component comprises a stirring rod 7, a stirring driving mechanism 6, a connecting seat, a telescopic rod 8 and a telescopic driving mechanism, wherein one end of the telescopic rod 8 is in driving connection with the telescopic driving mechanism to form a telescopic part, the telescopic part is arranged in the treatment tank 1, the other end of the telescopic rod 8 extends towards the inner cavity of the treatment tank 1, the end part of the telescopic rod extends out of the inner cavity of the treatment tank 1, the end part of the telescopic rod is connected with the connecting seat, the connecting seat supports the stirring driving mechanism 6, the stirring driving mechanism 6 is in driving connection with one end of the stirring rod 7, and the other end of the stirring rod 7 extends towards the inner cavity of the treatment tank 1 so as to stir raw materials stored in the treatment tank 1;
through the mutual coordination between the stirring component and the enzyme releasing component, the reaction efficiency of the biological enzyme and the raw material can be accelerated in the stirring process, and the efficiency of releasing the cell content containing the target protein peptide from the raw material can be accelerated;
the active ingredient stabilizing unit includes a temperature adjusting means for controlling the temperature in the treatment tank 1 to prevent the degradation of the cell contents containing the target protein peptide due to an excessively high temperature or the decrease of the activity due to an excessively low temperature, a PH adjusting means for adjusting the PH of the raw material in the treatment chamber, and an additive feeding means for feeding the stabilizer and the antioxidant into the treatment tank 1;
In this embodiment, the treatment tank 1 is configured in a double-layer structure, that is, the treatment tank 1 is provided with an interlayer 9, and the temperature adjusting member is disposed in the interlayer 9 of the treatment tank 1 to heat the inner layer of the treatment tank 1 and maintain accurate temperature control of the cell content containing the target protein peptide;
the temperature regulating component comprises a heating element 2, a treatment tank temperature sensor and a current controller, wherein the current controller is used for controlling current applied to the heating element 2, the treatment tank temperature sensor is used for collecting temperature data in the treatment tank 1, the heating element 2 is arranged along the interlayer 9 of the treatment tank 1 in a surrounding way, so that the heating element 2 is used for heating the interlayer 9, and the temperature of the raw materials in the treatment tank 1 is regulated;
wherein in the present embodiment, the temperature adjusting means further comprises a heating liquid which is provided in the interlayer and which conducts heat emitted from the heat generating member into the treatment tank 1, thereby adjusting the temperature of the raw material in the treatment tank 1;
the PH value adjusting component comprises a PH meter, a first storage tank 5, a second storage tank 11, a first electronic control valve 12 and a second electronic control valve, wherein the PH meter is arranged in the treatment tank 1 to detect the PH value of raw materials in the treatment tank 1, the first storage tank 5 stores an acid regulator, the second storage tank 11 stores an alkaline regulator, the first electronic control valve 12 is arranged at the outlet of the first storage tank 5 and controls the application amount of the acid regulator, and the second electronic control valve is arranged at the outlet of the second storage tank 11 and controls the application amount of the alkaline regulator;
Wherein, the first electronic control valve 12 and the second electronic control valve have flow monitoring functions, thereby realizing accurate control of the application amounts of the acid regulator and the alkaline regulator;
meanwhile, a closed loop control is formed among the PH meter, the first electronic control valve 12, the second electronic control valve and the central processing unit, so that the PH value in the treating tank 1 can be accurately controlled;
in the present embodiment, the PH meter is provided in the treatment tank 1 without affecting the stirring position of the stirring member, and preferably, the PH meter is provided in the inner wall of the treatment tank 1;
the additive throwing component comprises a third storage tank, an electronic throwing valve and an additive stored in the third storage tank, wherein the electronic throwing valve is arranged at a throwing port of the third storage tank and is used for throwing the additive stored in the third storage tank into the processing cavity;
the central processing unit controls the electronic release valve to act, so that the additive in the third storage tank can fall into the treatment tank 1, and accurate control of the additive in the third storage tank is realized;
in the example, the electronic dispensing valve dispenses a set proportion of additive each time; in this example, the stated set ratio is the ratio of stabilizer to antioxidant in the additive;
The protein peptide extraction module comprises an extraction tank 13, an ultrasonic auxiliary extraction unit and a selective adsorption unit, wherein the ultrasonic auxiliary extraction unit and the selective adsorption unit are arranged in the extraction tank 13, the ultrasonic auxiliary extraction unit is used for carrying out auxiliary release on protein peptide in the pretreated raw material, and the selective adsorption unit selectively adsorbs target protein peptide;
in the present embodiment, the treatment tank 1 is connected to the extraction tank 13 through a connection pipe 10, as shown in fig. 6 and 7;
the ultrasonic auxiliary extraction unit and the selective adsorption unit are both arranged in the extraction tank 13 and used for carrying out auxiliary release on the protein peptide in the pretreated raw material, so that more target protein peptide in the raw material is ensured to be released, and the utilization rate of the raw material is improved;
the ultrasonic auxiliary extraction unit comprises a lifting member, an ultrasonic generator 15 and an ultrasonic probe, wherein the lifting member adjusts the positions of the ultrasonic generator 15 and the ultrasonic probe so as to realize auxiliary release of different positions in the extraction tank 13, the ultrasonic generator 15 is used for generating high-frequency ultrasonic waves, and the ultrasonic probe is contacted with liquid in the extraction tank 13 and directly transmits the ultrasonic waves generated by the ultrasonic generator 15 into a raw material solution;
The lifting member comprises a lifting rod 14, a lifting driving mechanism and a height detecting piece, one end of the lifting rod 14 is connected with the ultrasonic generator 15 and the ultrasonic probe, the other end of the lifting rod 14 is in driving connection with the lifting driving mechanism, and the height detecting piece detects the lifting height of the lifting rod 14 so as to obtain the lifting amount of the lifting rod 14;
the lifting rod 14 is arranged to be telescopic, and is driven by the lifting driving mechanism to realize telescopic action, so that the auxiliary release of the protein peptide in the pretreated raw material is realized, and meanwhile, when the lifting rod 14 is at different depths, the auxiliary release of the protein peptide in the pretreated raw material at different depth positions can be realized;
through the mutual matching of the ultrasonic auxiliary extraction unit and the selective adsorption unit, the protein peptide in the raw material can be fully released, the utilization rate of the raw material is improved, and the whole system has the advantage of high raw material utilization rate;
the selective adsorption unit comprises an adsorption column 19, an adsorption material 29, a sample injection member, a temperature control member 28 and an elution member, wherein the adsorption material 29 is filled in the adsorption column 19, the sample injection member inputs the pretreatment liquid containing protein peptide into the adsorption column 19, the temperature control member 28 maintains the temperature in the adsorption column 19, and the elution member is used for eluting target protein peptide from the adsorption material 29 after adsorption;
Optionally, the adsorption material 29 comprises ion exchange resin, affinity chromatography medium, molecularly imprinted polymer; meanwhile, according to the material of the protein peptide to be processed, an adaptive adsorption material 29 is selected, so that the identification and the processing of the specific peptone (target protein peptide) are realized;
as shown in fig. 10, the adsorption material 29 is clamped on one side of the permeable membrane 26, and the permeable membrane 26 is arranged on the periphery of the adsorption column 19 for exudation of non-target protein peptides;
at the same time, non-target protein peptides permeate the adsorption material 29 and enter the treatment tank 1 for storage (this part is waste, not required by the current process, and can be discarded), and target protein peptides remain on the adsorption material 29;
optionally, the sample feeding member includes a transfer pump, a control valve 17, a transfer pipe 16, and a flow meter, where the transfer pump provides a flow rate and a pressure to ensure transfer of the pretreatment liquid, one end of the transfer pipe 16 is connected to the extraction tank 13, and the other end of the transfer pipe 16 extends to the adsorption column, preferably may extend into the adsorption column or may be suspended above the adsorption column 19, so as to supply the pretreatment liquid to the adsorption column 19, and the flow meter is used to detect a flow rate in the transfer pipe 16, and the control valve 17 performs on-off control on the transfer pipe 16;
Wherein the delivery pump is arranged in the transmission pipeline 16 and transmits the pretreatment liquid through the transmission pipeline 16;
meanwhile, the control valve 17 is set to be electronic and is opened and closed based on a control signal of the central processing unit;
after the raw materials in the treatment tank 1 are treated, the raw materials are transferred to an adsorption column 19 of the extraction tank 13 through a sample injection member;
optionally, the temperature control member 28 includes a heater, a cooler, and a cooler temperature sensor, the heater, the cooler being disposed on a side wall of the adsorption column 19, and the heater and the cooler being staggered;
the cooler temperature sensor is arranged on the inner wall of the cooler and collects temperature data in the cooler;
in this embodiment, the temperature of the adsorption process of the protein peptide is monitored by the temperature control member 28, so that the stability of the activity of the protein peptide is ensured, and the change of the activity of the protein peptide caused by the temperature loss of the protein peptide is prevented;
the selective adsorption unit further comprises a switching member and an elution tank 23, the switching member transferring the adsorption column 19 from the treatment tank 1 into the elution tank 23, when the switching member transfers the adsorption column 19 into the elution tank 23, the elution member is triggered to rinse the adsorption column 19;
The conversion member comprises a conversion seat, a conversion rod 22, a conversion driving mechanism 21, a lifting rod 20 and a lifting mechanism, wherein one end of the conversion rod 22 is in driving connection with the conversion driving mechanism 21 to form a conversion part, the conversion part is arranged on the conversion seat, one end of the lifting rod 20 is in driving connection with the lifting driving mechanism to form a lifting part, the lifting part is arranged at the end part of the other end of the conversion rod 22, one end of the lifting rod 20 extends towards one side far away from the lifting part, and the adsorption column 19 is arranged at the end part of the lifting rod 20;
wherein the axis of the lifting rod 20 is perpendicular to the axis of the switching rod 22;
the lifting rod 20 is arranged to be telescopic, and is driven by the lifting driving mechanism to realize telescopic action, so that telescopic action is realized;
in the present embodiment, the eluting tank 23 is provided at one side of the treatment tank 1, and the switching means can reciprocally switch the adsorption column 19 between the treatment tank 1 and the eluting tank 23;
the eluting tank 23 and the side wall of the treatment tank 1 are respectively provided with a first channel 24 and a second channel 25 for the adsorption column 19 to pass through, when the pretreatment liquid is treated, the adsorption column 19 is lifted and lowered under the lifting operation of the lifting rod 20, is restored to the original position (the original position is set to be flush with the first channel 24 and the second channel 25, and the adsorption column 19 can just pass through the first channel 24 and the second channel 25), and enters the eluting tank 23 under the conversion of the conversion driving mechanism 21 to the conversion rod 22, and the target protein peptide adsorbed on the adsorbing material 29 is washed down by the eluting member, so that the extracted target protein peptide is stored in the eluting tank 23;
Wherein the elution member is disposed within the adsorption column 19 and cleans the adsorption column 19 after the adsorption column 19 enters the elution tank 23;
optionally, the eluting member includes a liquid storage tank, a spray head, a supply pump, and an eluting pipe, the liquid storage tank stores an eluent, the supply pump is disposed at one end of the eluting pipe and forms a supply part together with the eluting pipe, the supply part is disposed in the liquid storage tank and supplies the eluent in the liquid storage tank, the spray head is disposed at the other end of the eluting pipe and sprays the eluent onto the adsorption column 19 to flush the adsorbed target protein peptide;
in addition, the eluting means further comprises an electronic opening valve 18, the electronic opening valve 18 is arranged at the bottom wall of the adsorption column 19, and when the target protein peptide on the adsorption material 29 is washed, the target protein peptide is opened, so that the target protein peptide can be smoothly washed and enter the washing tank;
optionally, the quality control module includes a detection unit and an analysis unit, the detection unit collects state data of the target protein peptide adsorbed in the adsorption column 19, the detection unit collects state data of the protein peptide in the adsorption column 19, and the analysis unit analyzes the target protein peptide according to the state data collected by the detection unit;
Wherein the status data comprises spectral characteristics of the target protein peptide;
the detection unit is arranged in the adsorption column 19, and comprises at least one spectrum detector 30 and a placing seat, wherein the placing seat is used for placing the spectrum detector 30, and the at least one spectrum detector 30 collects state data of the target protein peptide;
the status data includes, but is not limited to, the following list: monitoring the absorption intensity at a wavelength of 280nm, the absorption intensity value at a wavelength of 260 nm;
in addition, at least one spectrum detector 30 is symmetrically disposed on the inner wall of the adsorption column 19, and collects the absorption intensity data of the target protein peptide adsorbed in the adsorption column 19;
meanwhile, the spectrum detector 30 is specifically an ultraviolet-visible spectrum detector;
optionally, the analysis unit obtains the spectral characteristics of the target protein peptide and calculates the Purity index Purity of the target protein peptide according to the following formula:
；
wherein A is 280 A is the absorption intensity value of the extracting solution at the spectral wavelength of 280nm 260 For the absorption intensity value of the extracting solution at the spectral wavelength of 260nm, CF is a correction factor, the value of which is an empirical value, and is determined according to the purified target protein historical data;
If the Purity index Purity of the target protein peptide is lower than a monitoring threshold Range set by a system, adjusting the sample injection speed of the sample injection member and the temperature of the temperature control member;
if the Purity index Purity of the target protein peptide is higher than a monitoring threshold Range set by a system, the sample injection speed of the sample injection member and the temperature of the temperature control member meet the set requirements, and special adjustment is not needed;
in this embodiment, a manner of determining the correction factor is provided, and the following is a step of determining CF:
s1, preparing a standard solution
Preparing standard solutions of pure proteins and other substances (e.g., nucleic acids) of known concentrations;
s2, measuring an absorption spectrum:
measuring the absorbance of the protein and nucleic acid standard solutions at specific wavelengths (e.g., 280 nm and 260 nm), respectively, using an ultraviolet spectroscopy detector;
s3, calculating an independent absorption ratio:
for the protein, the absorption ratio was calculated at 280 nm and 260 nm;
for other substances (represented by nucleic acids, for example), the absorption ratios at 260 nm and 280 nm were also calculated;
s4, determining CF:
determining CF using the uptake ratio of the nucleic acid; CF is the ratio of nucleic acid uptake at 280 nm relative to its uptake at 260 nm;
Suppose A Nucleic acid 280 And A Nucleic acid 260 The absorbance of the nucleic acid at 280 nm and 260 nm, respectively, then the formula for CF is:
；
through the mutual coordination between the quality control module and the protein peptide extraction module, the extraction efficiency and the recognition capability of the target protein peptide are improved, and the whole system is ensured to have the advantages of high extraction stability, high extraction quality and high activity in the extraction process;
optionally, the purification module comprises a purification unit and a concentration unit, the purification unit further purifies the extracting solution to form primary treatment solution, and the concentration unit is used for removing small molecular impurities in the primary treatment solution so as to adjust the concentration of the primary treatment solution to form purified solution;
the purification unit includes a chromatographic column 34, a supply member to allow packing or medium filled in the chromatographic column 34, the supply member injecting an extraction liquid into the chromatographic column 34 and purifying the extraction liquid through the chromatographic column 34;
the purification unit further comprises the purification tank 33, wherein the purification tank 33 is used for supporting the chromatographic column 34 and the purified target protein peptide;
the supply member comprises a delivery pump, a supply pipe 31 and an electronic supply valve 32, wherein the delivery pump is used for delivering the extracting solution into the chromatographic column 34, one end of the supply pipe 31 is connected with the delivery pump, the other end of the supply pipe 31 extends into the chromatographic column 34, and the electronic supply valve 32 is used for controlling the on-off of the supply pipe 31 so as to control the amount of the extracting solution entering the chromatographic column 34;
As shown in fig. 13, a semipermeable membrane 36 is disposed in the chromatographic column 34, and after the semipermeable membrane 36 filters the extract once, the filtered extract can be concentrated and filtered by the concentrating unit, so as to implement the re-purification of the extract to obtain purified protein peptide;
in this example, the semipermeable membrane 36 is selected according to the type of the target protein peptide actually required, and this is a technical means well known to those skilled in the art, so that details are not repeated in this example;
the concentration unit comprises an ultrafiltration member and a centrifugal member, wherein the ultrafiltration member filters the purified protein peptide, and the centrifugal member centrifugally concentrates the filtered protein peptide;
the ultrafiltration member comprises a supporting frame and a filtering membrane 37, the supporting frame supports the filtering membrane 37 to form a filtering part, and the filtering part is clamped on the inner wall of the purification tank 33 and filters the purified protein peptide;
the centrifugal component is arranged below the ultrafiltration component and is used for centrifuging the filtered protein peptide so as to promote the purity of the target protein peptide to be higher;
The ultrafiltration member further comprises a withdrawal station 42, the centrifugation member being arranged on the withdrawal station 42 such that the withdrawal station 42 is withdrawn after centrifugation is completed to obtain a purified target protein peptide;
meanwhile, the extraction mode of the extraction stage 42 includes mechanical extraction or sliding extraction, which is well known to those skilled in the art, so that a detailed description is omitted in this embodiment;
the centrifuge member comprises a centrifuge tube and a centrifuge drive mechanism 41, the centrifuge drive mechanism 41 driving the centrifuge tube to cause the small molecule components to be filtered out through the filter membrane 37 of the centrifuge tube;
in this embodiment, a filtering membrane 37 is provided on the side wall of the centrifuge tube, and the centrifuge tube is driven by the centrifugal driving mechanism 41 to centrifuge the filtered protein peptide, so that larger protein molecules (target protein peptide) cannot pass through and are retained in the tube; concentrating the protein peptide in a centrifuge tube, and simultaneously removing small molecular impurities in the solution;
in this embodiment, the filtering membrane 37 is set as a selectively permeable membrane, the pore size of the membrane is smaller than the size of the target protein peptide, and an adaptive filtering membrane 37 is selected according to the type of the target protein peptide actually required, which is a technical means well known to those skilled in the art, so that details are not repeated in this example;
When the concentration unit is finished, two parts are obtained, wherein one part is concentrated solution containing high-concentration target protein peptide and is retained in a centrifuge tube; the other part is the dialyzate from which the macromolecular proteins are removed, and the dialyzate contains water and other small molecular impurities;
obtaining the target protein peptide retained in the centrifuge tube, so as to realize high-purification protein peptide;
through the mutual coordination of the purification module and the protein peptide extraction module, the target protein peptide can be purified, the purification efficiency and the purification precision of the target protein peptide are improved, and the whole system has the advantages of accurate protein peptide identification, high extraction stability and good purification stability;
optionally, the evaluation module includes a sampling unit and an evaluation unit, the sampling unit collects purification data of the purification process of the purification unit, and the evaluation unit evaluates the purification process according to the purification data collected by the sampling unit;
the sampling unit comprises a first ultraviolet-spectrum detector 35, a PH sensor, a conductivity sensor, an extracting solution temperature sensor and a data storage, wherein the first ultraviolet-spectrum detector 35 collects the ultraviolet absorption value data of the extracting solution after purification, the PH sensor collects the PH value data of the extracting solution after purification, the conductivity sensor collects the purified extracting solution conductivity data, the extracting solution temperature sensor collects the temperature data of the extracting solution after purification, and the data storage stores the ultraviolet absorption value data collected by the first ultraviolet-spectrum detector 35, the PH value data collected by the PH sensor, the purified extracting solution conductivity data collected by the conductivity sensor and the temperature data of the extracting solution after purification;
Optionally, the evaluation unit acquires the purified data acquired by the sampling unit, and calculates the state index ACT according to the following formula:
；
in the UV norm (T) is the normalized value of the ultraviolet absorption value of the T-th time node in the sampling period T, pH norm Cond for normalization of pH norm T is the normalized value of conductivity norm Is the standard value of temperature, a, b, c, d is the adjustment coefficient, and is adjusted according to experimental data and specific application, t 0 For the start time of the purification process, T is the end time at which the purification process ends;
t in sampling period T 0 Normalization of UV absorbance values for time nodesValue UV norm The calculation is performed according to the following formula:
；
in the UV actual Is the T in the sampling period T 0 The actual measured UV absorption value of the time node, which value is detected by the first UV-spectrum detector, UV min And UV max Respectively the minimum and maximum ultraviolet absorption values preset in the purification process;
standardization of pH value pH norm The calculation is performed according to the following formula:
；
in the pH value actual For the actual measured pH value, the value is detected by a pH sensor, the pH min To a preset minimum pH value in the purification process, pH max The pH value is the preset maximum pH value in the purification process;
normalized value of conductivity Cond norm The calculation is performed according to the following formula:
；
In Cond actual For actually measured conductivity values, the value is detected by a conductivity sensor, cond min Cond for a predetermined minimum conductivity value during purification max Is a predetermined maximum conductivity value during the purification process;
normalized value T of temperature norm The calculation is performed according to the following formula:
；
wherein T is actual For the actual measured temperature value, the value is detected by a conductivity sensor, T min T for a predetermined minimum temperature value during purification max Is a predetermined maximum temperature value during the purification process;
triggering the adjustment of the supply speed of the supply component if the state index ACT is lower than a state threshold Monitor set by a system;
if the state index ACT is higher than a state threshold Monitor set by the system, indicating that the current supply quantity of the supply component meets the set requirement;
the state threshold Monitor set by the system is set by a manager or the system, which is a technical means well known to those skilled in the art, and those skilled in the art can query related technical manuals to obtain the technology, so that the description is omitted in this embodiment;
a, b, c, d is provided in this embodiment as an adjustment coefficient, and a value example of adjustment according to experimental data and specific applications is as follows:
(1) The UV absorbance is most critical (assuming that the amount of protein is of most concern in the purification process, and that changes in pH and conductivity have relatively little effect on the purification process)
The allocation of the adjustment coefficients is as follows:
a=0.5: the influence of the ultraviolet absorption value is the greatest;
b=0.3: temperature stability is inferior;
c=0.1: the influence of conductivity is relatively small
d=0.1: the influence of the pH value is minimal;
(2) The pH value is as important as the UV absorption value (assuming that the stability of the protein is very sensitive to pH value, and also the quantitative purification effect)
The weight coefficient is distributed as follows:
a=0.3；
b=0.1；
c=0.3；
d=0.3；
(3) Average partition (all parameters are considered to have the same effect on the purification process)
The allocation of the adjustment coefficients is as follows:
a=0.25；
b=0.25；
c=0.25；
d=0.25；
in summary, the specific value of the adjustment coefficient can be set according to the actual requirement, so in this embodiment, details are not repeated;
through the mutual cooperation of the purification module and the evaluation module, the evaluation of the purification process is more accurate and reliable, and the whole system is ensured to have the advantages of accurate quality evaluation, high protein peptide purification efficiency and high protein peptide stability.
Embodiment two: this embodiment should be understood to include all the features of any one of the foregoing embodiments, and be further modified on the basis thereof, as shown in fig. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 13, further including a concentrating sampling subunit and a concentrating analysis subunit, where the concentrating sampling subunit samples a concentrating process, and the concentrating analysis subunit analyzes a centrifugal concentrating process according to data sampled by the concentrating sampling subunit to form an analysis result, and triggers control of a centrifugal speed of the purified extract by the centrifugal member according to the analysis result;
The concentration sampling subunit comprises a second ultraviolet-spectrum detector 38, a centrifuge tube temperature sensor 39, a rotating speed sensor and a flowmeter 40, wherein the second ultraviolet-spectrum detector is used for collecting ultraviolet absorption characteristics of liquid in the centrifuge tube, the centrifuge tube temperature sensor 39 is used for detecting the temperature in the centrifuge tube, the rotating speed sensor is used for collecting the centrifugal speed of the centrifuge tube, and the flowmeter 40 is used for collecting the flow rate of extracting solution in the centrifugal concentration process;
wherein the second ultraviolet-spectrum detector 38, the centrifuge tube temperature sensor 39 and the flowmeter 40 are arranged on the end surface of the supporting frame contacted with the extracting solution, and the data of the centrifuging process of the centrifuging component are sampled;
the rotating speed sensor is arranged on the outer wall of the centrifugal tube so as to detect the rotating speed of the centrifugal tube;
the concentration analysis subunit acquires the data acquired by the concentration sampling subunit, and calculates a judgment index Level of the concentration process of the purified extracting solution according to the following formula:
；
wherein lambda is 1 The weight coefficient of ultraviolet absorption is set by the system, lambda 2 Is the weight coefficient of temperature, the value of which is set by the system, lambda 3 The value of the weight coefficient is set by the system, lambda 4 Is the weight coefficient of the flow velocity, the value of which is set by the system, G norm Te is the standardized value of ultraviolet absorption value in the concentration process of the purified extract norm S is the normalized value of the temperature in the centrifuge tube norm Is the standardized value of the centrifugal speed of the centrifugal tube, F norm Is a standardized value of the flow rate of the extracting solution in the centrifugal concentration process;
normalized value for UV absorbance G norm Calculated according to the following formula:
；
wherein G is actual For the actual measured UV absorption value, U min And U max Predetermined minimum and maximum values for ultraviolet absorption;
normalized value Te for temperature norm Calculated according to the following formula:
；
in Te (Te) actual Is the actual measured temperature, te, in the centrifuge tube min And Te (Te) max Is a predetermined minimum and maximum value of temperature in the centrifuge tube;
for S norm The following formula is calculated for the normalized value of the rotation speed:
；
wherein S is actual For the actually measured rotational speed S min Is a preset minimum value of the rotating speed, S max For the purpose of speed of rotationSetting a maximum value;
for F norm Calculated for the normalized value of flow rate as follows:
；
wherein F is actual F for the actual measured flow rate min Is a predetermined minimum value of the flow velocity, F max Is a predetermined maximum of flow rate;
in this embodiment, the weight coefficient is typically between 0 and 1, and the sum of all weight coefficients should be equal to 1 to ensure that the contribution of each parameter is balanced;
Meanwhile, in this embodiment, a value example of a weight coefficient is provided, and specifically:
1) The most critical ultraviolet absorbance value
λ 1 =0.5；
λ 2 =0.2；
λ 3 =0.2；
λ 4 =0.1；
2) Temperature regulation is of paramount importance
λ 1 =0.2；
λ 2 =0.5；
λ 3 =0.2；
λ 4 =0.1；
3) The rotation speed and the temperature are equally important
λ 1 =0.25；
λ 2 =0.25；
λ 3 =0.25；
λ 4 =0.25；
4) The flow velocity has obvious influence on the process
λ 1 =0.1；
λ 2 =0.2；
λ 3 =0.3；
λ 4 =0.4；
5) Balanced but biased against UV absorption and flow rate
λ 1 =0.35；
λ 2 =0.15；
λ 3 =0.15；
λ 4 =0.35；
In a word, the setting of the weight coefficient needs to be adjusted according to the characteristics of the protein, the purpose of experiments and the past experience, and meanwhile, the purpose of weight distribution is to ensure that the Level index can accurately reflect the efficiency and quality of the whole concentration process; different experimental conditions and purposes may require different weight distribution strategies;
if the judgment index Level of the purified extracting solution concentration process is higher than a monitoring threshold Range' set by a system, triggering to control the centrifugal speed of the centrifugal driving mechanism;
if the judgment index Level of the concentration process of the purified extracting solution is lower than a monitoring threshold Range' set by a system, continuing to monitor the centrifugal tube (without triggering to control the centrifugal speed);
the monitoring threshold Range' set by the system is set by the system or the manager according to the actual situation, which is a technical means well known to those skilled in the art, and those skilled in the art can query the related technical manual to obtain the technology, so that the description is omitted in this embodiment;
The concentration analysis subunit acquires a judgment index Level of the concentration process of the purified extracting solution and a monitoring threshold Range set by a system, and calculates the driving speed V of the centrifugal driving mechanism according to the following formula:
；
wherein V is init The initial centrifugal speed or the reference centrifugal speed, the value of which is set by the system, k is an adjusting coefficient for controlling the sensitivity of the speed adjustment according to the difference between Level and Range, and the value of which is obtained according to experience or historical data;
when Level is greater than Range, meaning that the concentration effect exceeds that desired, it is necessary to slow down the centrifugation speed to prevent excessive concentration or protein damage;
when the Level is smaller than or equal to Range, the concentration effect accords with the set standard, and the current centrifugal speed is maintained to improve the concentration efficiency;
in this embodiment, a value example of the adjustment coefficient k is provided, specifically as follows:
1) Sensitive to the protein peptide concentration process (in which case the protein may be very sensitive to centrifugal speed variations, requiring fine tuning)
The k is lower, for example, k=500, and a smaller k means that the centrifugal speed is adjusted mildly even when there is a significant difference between Level and Range;
2) The need for rapid adjustment (if the protein is relatively stable and experimental conditions allow for faster adjustment)
The value of k is higher, for example, k=1500, and the larger value of k allows the centrifugal speed to be quickly adjusted when Level deviates significantly from Range, so as to quickly achieve the ideal concentration effect;
3) Balance adjustment (protein has a certain tolerance to the variation of centrifugal speed)
k takes a medium value, for example k=1000, a medium k value providing a balance between adjusting the speed and maintaining protein stability;
4) Requirements for specific proteins (based on the characteristics of the specific proteins and past experimental data)
Customizing a specific k value according to experimental data, such as k=800, according to experimental data and protein characteristics, to maximize the centrifugation process;
in summary, the determination of the k-value should be optimized by a series of preliminary experiments based on specific experimental conditions and characteristics of the target protein, and furthermore, the k-value should be set in consideration of limitations of the centrifuge apparatus and safety of the sample;
through mutually supporting between centrifugal component, concentrated sampling subunit and the concentrated analysis subunit for the purification concentration efficiency of the extract after the purification is more accurate, still compromise to the protection of protein peptide activity, guarantee that entire system has the advantage that the purification protein peptide activity is good and purification stability is high.
The foregoing disclosure is only a preferred embodiment of the present invention and is not intended to limit the scope of the invention, so that all equivalent technical changes made by applying the description of the present invention and the accompanying drawings are included in the scope of the present invention, and in addition, elements in the present invention can be updated as the technology develops.
Claims (10)
1. A protein peptide extraction and purification system for enhancing immune function, the protein peptide extraction and purification system comprising a server, a raw material and a processing tank, characterized in that the protein peptide extraction and purification system further comprises a raw material pretreatment module, a protein peptide extraction module, a quality control module, a purification module and an evaluation module, wherein the server is used for storing intermediate data and process data of the raw material pretreatment module, the protein peptide extraction module, the quality control module, the purification module and the evaluation module, and the raw material pretreatment module is arranged in the processing tank and is used for pretreating the raw material;
the raw material pretreatment module is used for pretreating raw materials to form pretreatment liquid, the protein peptide extraction module is used for extracting target protein peptide of the pretreatment liquid to form extraction liquid, the quality control module is used for collecting target protein peptide extraction process, analyzing the collected state data to form analysis result and dynamically adjusting the extraction process, the purification module is used for purifying the extraction liquid, the evaluation module is used for collecting the purification data of the purification process of the purification module, evaluating the purification process according to the purification data to form evaluation result and triggering the purification module to adjust the purification process according to the evaluation result;
The protein peptide extraction module comprises an extraction tank, an ultrasonic auxiliary extraction unit and a selective adsorption unit, wherein the ultrasonic auxiliary extraction unit and the selective adsorption unit are arranged in the extraction tank, the ultrasonic auxiliary extraction unit is used for carrying out auxiliary release on protein peptide in the pretreated raw material, and the selective adsorption unit selectively adsorbs target protein peptide;
the selective adsorption unit comprises an adsorption column, an adsorption material, a sample injection member, a temperature control member and an elution member, wherein the adsorption column is filled with the adsorption material, the sample injection member inputs the pretreatment liquid containing the target protein peptide into the adsorption column, the temperature control member maintains the temperature in the adsorption column, and the elution member is used for eluting the target protein peptide from the adsorption material after adsorption.
2. The immune function enhancing protein peptide extraction and purification system according to claim 1, wherein the sample injection member comprises a transfer pump, a control valve, a transfer pipe, a flow meter, wherein the transfer pump provides a flow rate and a pressure to ensure transfer of the pretreatment liquid, one end of the transfer pipe is connected with the extraction tank, the other end of the transfer pipe extends to the adsorption column to supply the pretreatment liquid into the adsorption column, the flow meter is used for detecting a flow rate in the transfer pipe, and the control valve performs on-off control on the transfer pipe;
The conveying pump is arranged in the conveying pipeline and conveys the pretreatment liquid through the conveying pipeline.
3. The protein peptide extraction and purification system for enhancing an immune function according to claim 2, wherein the quality control module comprises a detection unit and an analysis unit, wherein the detection unit collects state data of a target protein peptide in the adsorption column, and the analysis unit analyzes the target protein peptide according to the state data collected by the detection unit;
wherein the status data comprises spectral characteristics of the target protein peptide.
4. The immune function enhancing protein peptide extraction and purification system according to claim 3, wherein the analysis unit obtains the spectral characteristics of the target protein peptide and calculates the Purity index Purity of the target protein peptide according to the following formula:
；
wherein A is 280 A is the absorption intensity value of the extracting solution at the spectral wavelength of 280nm 260 For the absorption intensity value of the extracting solution at the spectral wavelength of 260nm, CF is a correction factor, the value of which is an empirical value, and is determined according to the purified target protein historical data;
and if the Purity index Purity of the target protein peptide is lower than a monitoring threshold Range set by a system, adjusting the sample injection speed of the sample injection member and the temperature of the temperature control member.
5. The system for extracting and purifying protein peptide having enhanced immune function according to claim 4, wherein the purification module comprises a purification unit and a concentration unit, the purification unit further purifies the extract to form a primary treatment liquid, and the concentration unit is used for removing small molecular impurities in the primary treatment liquid so as to adjust the concentration of the primary treatment liquid to form a purified liquid;
the purification unit includes a chromatographic column, a supply member injecting an extraction liquid into the chromatographic column, and purifying the extraction liquid through the chromatographic column, and a packing or medium filled in the chromatographic column.
6. The protein peptide extraction and purification system for enhancing an immune function according to claim 5, wherein the evaluation module comprises a sampling unit and an evaluation unit, wherein the sampling unit collects purification data of a purification process of the purification unit, and the evaluation unit evaluates the purification process according to the purification data collected by the sampling unit;
the sampling unit comprises a first ultraviolet-spectrum detector, a PH sensor, a conductivity sensor and a data storage, wherein the first ultraviolet-spectrum detector is used for collecting ultraviolet absorption value data of the purified extracting solution, the PH sensor is used for collecting PH value data of the purified extracting solution, and the conductivity sensor is used for collecting the obtained purified extracting solution conductivity data; the data memory stores the ultraviolet absorption value data, the PH value data and the extracting solution conductivity data.
7. The immune function enhancing protein peptide extraction and purification system according to claim 6, wherein said temperature control means comprises a heater, a cooler and a cooler temperature sensor, said heater, said cooler being disposed on a side wall of said adsorption column, and said heater and said cooler being staggered;
the cooler temperature sensor is arranged on the inner wall of the cooler and collects temperature data in the cooler.
8. The system for extracting and purifying protein peptide having an enhanced immune function according to claim 7, wherein the eluting means comprises a reservoir for storing an eluent, a spray head provided at one end of the eluting tube to form a supply part, and a supply pump provided in the reservoir to supply the eluent in the reservoir, and an eluting tube provided at the other end of the eluting tube to spray the eluent onto an adsorption column to flush the adsorbed target protein peptide.
9. The system for extracting and purifying protein peptides with enhanced immune function according to claim 8, wherein the evaluation unit obtains the purified data collected by the sampling unit, and calculates the state index ACT according to the following formula:
；
In the UV norm Is the standardized value of the ultraviolet absorption value, pH norm Cond for normalization of pH norm Is the normalized value of conductivity, w 1 Is the weight coefficient of the ultraviolet absorption value, the value of the weight coefficient is set by a system, and w 2 Is a pH value weight coefficient, the value of which is set by a system, w 3 Is a weight coefficient of the electrical conductivity,the value of which is set by the system.
10. The immune function enhancing protein peptide extraction and purification system of claim 9, wherein said adsorption material comprises ion exchange resin, affinity chromatography medium, molecularly imprinted polymer.
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