CN117603697A - Resistance-controlled antibiotic resistance gene pollution material composition, resistance-controlled pollution material, preparation method and application thereof - Google Patents
Resistance-controlled antibiotic resistance gene pollution material composition, resistance-controlled pollution material, preparation method and application thereof Download PDFInfo
- Publication number
- CN117603697A CN117603697A CN202311417225.2A CN202311417225A CN117603697A CN 117603697 A CN117603697 A CN 117603697A CN 202311417225 A CN202311417225 A CN 202311417225A CN 117603697 A CN117603697 A CN 117603697A
- Authority
- CN
- China
- Prior art keywords
- resistance
- water
- component
- quaternary ammonium
- ammonium salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000463 material Substances 0.000 title claims abstract description 81
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 42
- 230000003115 biocidal effect Effects 0.000 title claims abstract description 36
- 239000000203 mixture Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 29
- 239000011398 Portland cement Substances 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 20
- 239000004927 clay Substances 0.000 claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 17
- 238000003900 soil pollution Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- 238000002156 mixing Methods 0.000 claims description 16
- 238000011109 contamination Methods 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 229920002472 Starch Polymers 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 238000005096 rolling process Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 229960000892 attapulgite Drugs 0.000 claims description 3
- 239000000440 bentonite Substances 0.000 claims description 3
- 229910000278 bentonite Inorganic materials 0.000 claims description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 3
- JBIROUFYLSSYDX-UHFFFAOYSA-M benzododecinium chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 JBIROUFYLSSYDX-UHFFFAOYSA-M 0.000 claims description 3
- OCBHHZMJRVXXQK-UHFFFAOYSA-M benzyl-dimethyl-tetradecylazanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 OCBHHZMJRVXXQK-UHFFFAOYSA-M 0.000 claims description 3
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 claims description 3
- 229910052625 palygorskite Inorganic materials 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 3
- 229920005614 potassium polyacrylate Polymers 0.000 claims description 3
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 3
- 150000003863 ammonium salts Chemical group 0.000 claims description 2
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims description 2
- 125000002091 cationic group Chemical group 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 claims description 2
- -1 cyano quaternary ammonium salt Chemical class 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims description 2
- 150000003222 pyridines Chemical class 0.000 claims description 2
- 238000005067 remediation Methods 0.000 claims description 2
- 230000002068 genetic effect Effects 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 19
- 238000011282 treatment Methods 0.000 abstract description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 238000011065 in-situ storage Methods 0.000 description 3
- 239000003469 silicate cement Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 101150024821 tetO gene Proteins 0.000 description 3
- 101150004433 tetQ gene Proteins 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000005571 horizontal transmission Effects 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 229910000616 Ferromanganese Inorganic materials 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- WQHONKDTTOGZPR-UHFFFAOYSA-N [O-2].[O-2].[Mn+2].[Fe+2] Chemical compound [O-2].[O-2].[Mn+2].[Fe+2] WQHONKDTTOGZPR-UHFFFAOYSA-N 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 229940124350 antibacterial drug Drugs 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- DALUDRGQOYMVLD-UHFFFAOYSA-N iron manganese Chemical compound [Mn].[Fe] DALUDRGQOYMVLD-UHFFFAOYSA-N 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000009374 poultry farming Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
Landscapes
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of soil pollution treatment and restoration, and particularly discloses a resistance-control antibiotic resistance gene pollution material composition, a resistance-control pollution material, a preparation method and application thereof. The composition comprises a component A, a component B and Portland cement: (I) A component: clay, feSO 4 ·7H 2 O、KMnO 4 The method comprises the steps of carrying out a first treatment on the surface of the (II) component B: water-retaining agent and quaternary ammonium salt; (III) Portland cement. The resistance and control antibiotic resistance gene pollution material provided by the invention has the advantages of simple preparation and convenient use, and can be used for efficiently removing and long-acting resistance and control antibiotic resistance genes in soil.
Description
Technical Field
The invention relates to the technical field of soil pollution treatment and repair, in particular to a resistance-control antibiotic resistance gene pollution material composition, a resistance-control pollution material, a preparation method and application thereof.
Background
Antibiotics are widely used as an antibacterial drug in human medical treatment and livestock and poultry farming, however, excessive use of antibiotics induces the generation of antibiotic resistance genes (antibiotic resistance genes, ARGs) in animals, and causes a certain influence on the ecological environment after the discharge of feces, so that the harm of ARGs is far more than that of the antibiotics per se due to the persistence of ARGs residues, and the ARGs are popular as a novel environmental pollutant in various fields since 2006.
Soil is considered as one of the most important receptors for antibiotic residues, and the antibiotic residues and ARGs enter the soil through fertilization, sludge agriculture, waste water or reclaimed water irrigation and other modes, so that the soil becomes a huge reservoir of the ARGs, and the human health is seriously threatened. In order to reduce the spread of ARGs in soil media, researchers have focused on developing various ARGs abatement technologies, such as aerobic composting, anaerobic fermentation and water treatment processes, to reduce the input and enrichment of antibiotics and exogenous ARGs in soil caused by agricultural production processes such as organic fertilizer, sludge returning, sewage irrigation and the like.
In order to prevent the diffusion of ARGs entering the soil, researchers use a method (CN 202110270919.2) of adding biomass charcoal (CN 202110259591.4), agricultural ferment (CN 202111045142.6) and other materials into the soil and utilizing biomass charcoal-super-enriched plants to repair in situ.
However, the current in-situ repair method still has the problems of long repair period, insufficient resistance to ARGs horizontal transmission, and the like, and is difficult to popularize and apply. Therefore, searching or developing a repair material with excellent performance and economical and practical performance becomes a key for controlling ARGs pollution of soil by primary steric hindrance.
Disclosure of Invention
The invention aims to solve the problems of long repair period, insufficient resistance to ARGs horizontal transmission and control and the like in the in-situ repair method in the prior art.
In order to achieve the above object, a first aspect of the present invention provides a resistance-controlling antibiotic resistance gene pollution material composition comprising an a-component, a B-component and portland cement, wherein the material composition comprises the following components:
(I) And (3) a component A: clay, feSO 4 ·7H 2 O、KMnO 4 ；
(II) component B: water-retaining agent and quaternary ammonium salt;
(III) portland cement;
wherein the clay and the FeSO 4 ·7H 2 O, the KMnO 4 The content weight ratio of the water-retaining agent to the quaternary ammonium salt is 10-50:3-15:2-10:10-30:2-5;
the weight content ratio of the component A, the component B and the Portland cement is 5-10:3-6:0.5-1;
in the component A, the clay is selected from any one of attapulgite, bentonite or diatomite, and the average volume particle diameter is not more than 150 mu m.
In a second aspect, the present invention provides (1) a process for preparing material A: clay, feSO in the presence of water I 4 ·7H 2 O、KMnO 4 After first mixing, regulating the pH value to 7-8, then carrying out first contact reaction, and obtaining a material A after separation, washing and drying;
(2) Preparing a material B: mixing the aqueous solution of the quaternary ammonium salt with a water-retaining agent to perform a second contact reaction, and separating to obtain a material B;
(3) Preparing a resistance and antibiotic resistance gene pollution material: carrying out second mixing on the material A and Portland cement and water III to obtain a mixture I; wherein the amount of water II is controlled such that the water content of the mixture I is 60t%;
rolling and granulating the mixture I and the material B, and drying to obtain the resistance gene pollution material;
wherein the clay and the FeSO 4 ·7H 2 O, the KMnO 4 The weight ratio of the water-retaining agent to the quaternary ammonium salt is 10-50:3-15:2-10:10-30:2-5;
the weight ratio of the material A to the material B to the Portland cement is 5-10:3-6:0.5-1.
In a third aspect, the present invention provides a resistance-controlled antibiotic resistance gene contamination material prepared by the method of the second aspect.
The fourth aspect of the invention provides an application of the resistance-controlled antibiotic resistance gene pollution material in soil pollution control and restoration.
The resistance and control antibiotic resistance gene pollution material provided by the invention has the advantages of simple preparation and convenient use, and can be used for efficiently removing and long-acting resistance and control antibiotic resistance genes in soil.
Detailed Description
The endpoints and any values of the ranges disclosed herein are not limited to the precise range or value, and are understood to encompass values approaching those ranges or values. For numerical ranges, one or more new numerical ranges may be found between the endpoints of each range, between the endpoint of each range and the individual point value, and between the individual point value, in combination with each other, and are to be considered as specifically disclosed herein.
It should be noted that, in the aspects of the present invention, the present invention is described only once in one aspect thereof without repeated description with respect to the same components or terms in the aspects, and those skilled in the art should not understand the limitation of the present invention.
As described above, the first aspect of the present invention provides a resistance-controlling antibiotic resistance gene pollution material composition comprising a component a, a component B and portland cement, wherein the material composition comprises the following components:
(I) And (3) a component A: clay, feSO 4 ·7H 2 O、KMnO 4 ；
(II) component B: water-retaining agent and quaternary ammonium salt;
(III) portland cement;
wherein the clay and the FeSO 4 ·7H 2 O, the KMnO 4 The content weight ratio of the water-retaining agent to the quaternary ammonium salt is 10-50:3-15:2-10:10-30:2-5;
the weight content ratio of the component A, the component B and the Portland cement is 5-10:3-6:0.5-1;
in the component A, the clay is selected from any one of attapulgite, bentonite or diatomite, and the average volume particle diameter is not more than 150 mu m.
Preferably, in the component B, the water-retaining agent is at least one selected from cationic polyacrylamide and starch graft cross-linked matter thereof, sodium polyacrylate and starch graft cross-linked matter thereof, potassium polyacrylate and starch graft cross-linked matter thereof.
More preferably, in the component B, the water-retaining agent is sodium polyacrylate and/or potassium polyacrylate. The inventors found that in this preferred case, the control effect of the material against contamination with the antibiotic resistance gene can be made better.
Preferably, in the component B, the quaternary ammonium salt is at least one selected from dodecyl dimethyl benzyl ammonium chloride, dodecyl trimethyl ammonium chloride, dodecyl dimethyl benzyl ammonium bromide, tetradecyl dimethyl benzyl ammonium chloride, cyano quaternary ammonium salt, bis C8 alkyl quaternary ammonium bromide, polyazacyclic quaternary ammonium salt, pyridine derivative, polyquaternary ammonium salt, and bis quaternary ammonium salt.
More preferably, in the component B, the quaternary ammonium salt is at least one selected from dodecyl trimethyl ammonium chloride, tetradecyl dimethyl benzyl ammonium chloride, and dodecyl dimethyl benzyl ammonium chloride.
Preferably, the Portland cement is at least one selected from early strength Portland cements having a strength of PO32.5R to PO42.5R.
According to a preferred embodiment, the composition further comprises water.
As previously mentioned, the second aspect of the present invention provides a method for preparing a material for resistance to controlled antibiotic resistance gene contamination using the substance of the composition of the first aspect, comprising:
(1) Preparing a material A: clay, feSO in the presence of water I 4 ·7H 2 O、KMnO 4 After first mixing, regulating the pH value to 7-8, then carrying out first contact reaction, and obtaining a material A after separation, washing and drying;
(2) Preparing a material B: mixing the aqueous solution of the quaternary ammonium salt with a water-retaining agent to perform a second contact reaction, and separating to obtain a material B;
(3) Preparing a resistance and antibiotic resistance gene pollution material: carrying out second mixing on the material A and Portland cement and water III to obtain a mixture I; wherein the amount of water II is controlled such that the water content of the mixture I is 60t%;
rolling and granulating the mixture I and the material B, and drying to obtain the resistance gene pollution material;
wherein the clay and the FeSO 4 ·7H 2 O, the KMnO 4 The weight ratio of the water-retaining agent to the quaternary ammonium salt is 10-50:3-15:2-10:10-30:2-5;
the weight ratio of the material A to the material B to the Portland cement is 5-10:3-6:0.5-1.
Preferably, in step (1), the pH is adjusted using a NaOH solution having a concentration of 3M.
Preferably, in step (2), the concentration of the quaternary ammonium salt in the aqueous solution of the quaternary ammonium salt is 2 to 5wt%. The inventors found that in this preferred case, the removal rate of the resistance gene is more favorable.
Preferably, in step (1), the conditions of the first mixing at least satisfy: the stirring is carried out under the condition of 300-400rpm for 10-15min.
Preferably, in step (1), the conditions of the first contact reaction at least satisfy: the stirring is carried out under the condition that the temperature is 22-28 ℃, the rotating speed is 300-400rpm, and the time is 6-12h.
Preferably, in step (1), the washing is rinsing with deionized water.
Preferably, in step (1), the drying conditions at least satisfy: the temperature is 100-120 ℃ and the time is 4-6h.
According to a preferred embodiment, in step (2), the conditions of the second contact reaction at least satisfy: the stirring is carried out under the condition that the temperature is 22-28 ℃, the rotating speed is 300-400rpm, and the time is 4-8h.
Preferably, in step (3), the conditions of the second mixing at least satisfy: the stirring is carried out under the condition of 300-400rpm for 0.5-1h.
Preferably, in step (3), the temperature of the drying is 100-110 ℃.
In the present invention, the water I, the water II, and the water III are all water, and the weight of the water II, the weight of the water III, and the weight of the water in the aqueous solution of the quaternary ammonium salt together constitute the weight of the water I.
It should also be noted that the apparatus and conditions for the rolling granulation are not particularly limited by the present invention and may be carried out using conventional means in the art.
As previously described, a third aspect of the present invention provides a resistance-controlling antibiotic resistance gene contamination material prepared by the method of the second aspect described above.
As described above, the fourth aspect of the present invention provides the use of the resistance-controlled antibiotic resistance gene contaminated material according to the third aspect in soil pollution control and remediation.
Preferably, the adding amount of the resistance-controlled antibiotic resistance gene pollution material is 30-80g/m 2 。
Preferably, the resistance-controlling antibiotic resistance gene contamination material is applied to the soil to be treated, and the water content of the soil to be treated is maintained at 12-20wt%.
The invention will be described in detail below by way of examples. In the following examples, unless otherwise specified, all the raw materials used are commercially available.
Some of the raw materials and sources used in the examples below were as follows:
room temperature in the present invention means 25±2 ℃ unless otherwise specified.
Example 1
This example is for illustrating the method for preparing a resistance-controlled antibiotic resistance gene contamination material according to the present invention, referring to the amounts and parameters shown in Table 1, and is performed according to the procedure comprising the steps of:
(1) Preparing a material A: 10g of clay, feSO, in the presence of water II (deionized water) 4 ·7H 2 O、KMnO 4 After first mixing, regulating the pH to 7 by using a NaOH solution with the concentration of 3M, then carrying out a first contact reaction, eluting by using deionized water after separation, and drying to obtain a material A;
(2) Preparing a material B: mixing an aqueous solution of quaternary ammonium salt (the concentration of the quaternary ammonium salt is 5 wt%) with a water-retaining agent to perform a second contact reaction, and separating to obtain a material B;
(3) Preparing a resistance and antibiotic resistance gene pollution material: carrying out second mixing on the material A and Portland cement and water III to obtain a mixture I; wherein the amount of water III is controlled such that the water content of the mixture I is 60 wt.%;
the weight ratio of the material A to the material B to the silicate cement is 5:3:0.5;
and rolling and granulating the mixture I and the material B, and drying to obtain the resistance-controlled antibiotic resistance gene pollution material which is named as P1.
Example 2
This example uses a formulation and method similar to that of example 1 except that: the preparation conditions, the names of substances and the addition amount are changed, wherein the weight ratio of the material A to the material B to the silicate cement is 5:3.5:0.4;
referring specifically to Table 1, the resistance gene contamination material of the resistance control antibiotic, designated as P2, was finally obtained.
Example 3
This example uses a formulation and method similar to that of example 1 except that: the preparation conditions, the names of substances and the addition amount are changed, wherein the weight ratio of the material A to the material B to the silicate cement is 5:4:1, a step of;
referring specifically to Table 1, the resistance gene contamination material of the resistance control antibiotic, designated as P3, was finally obtained.
Test example 1
The prepared resistance-controlled antibiotic resistance gene contamination materials prepared in examples and comparative examples were prepared at a rate of 50g/m 2 The adding amount of the fertilizer is added into farmland soil with the thickness of 20cm, the farmland soil is uniformly mixed, and water is sprayed to ensure that the water content of the soil is 15%;
when P1 is used, the initial abundance of several major resistance genes tetO, tetQ, tetWsul 2, intI1 in soil is 2.55X10, respectively 6 copies/g、3.73×10 7 copies/g、1.17×10 7 copies/g、4.11×10 7 Copies/g and 3.14X10 7 copies/g；
When P2 is used, the initial abundance of several major resistance genes tetO, tetQ, tetWsul 2, intI1 in soil is 2.55X10, respectively 6 copies/g、3.73×10 7 copies/g、1.17×10 7 copies/g、4.11×10 7 Copies/g and 3.14X10 7 copies/g；
When P3 is used, the initial abundance of several major resistance genes tetO, tetQ, tetWsul 2, intI1 in soil are 3.12X10, respectively 7 copies/g、2.83×10 6 copies/g、2.45×10 7 copies/g、2.97×10 7 Copies/g and 5.11X10 7 copies/g；
After 7 days of stabilization treatment, the removal rates of the resistance genes in the above 5 were recorded, respectively, and the results are shown in Table 2.
Test example 2
The procedure was carried out in a similar manner to test example 1, except that a ferro-manganese oxide modified clay material was used instead of the resistance-controlling antibiotic resistance gene contamination material and was carried out in the same amount as the addition amount, and the removal rate of the resistance gene in the above 5 was recorded, and the results are shown in Table 2.
TABLE 1
TABLE 2
| Resistance gene species | P1 | P2 | P3 | Iron-manganese oxide modified clay material |
| tet O removal Rate (%) | 77.8 | 77.7 | 85.8 | 20 |
| tet Q removal Rate (%) | 83.5 | 85.3 | 86.1 | 25 |
| tet W removal Rate (%) | 82.7 | 85.9 | 90.5 | 23 |
| Sul 2 removal (%) | 90.4 | 91.8 | 92.8 | 27 |
| intI1 removal (%) | 92.8 | 91.9 | 90.9 | 25 |
The result shows that the resistance gene pollution material for the resistance antibiotic provided by the invention has the advantages of simple preparation, convenient use, and capability of efficiently removing and long-acting resistance control on the resistance gene in the soil.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, a number of simple variants of the technical solution of the invention are possible, including combinations of the individual technical features in any other suitable way, which simple variants and combinations should likewise be regarded as being disclosed by the invention, all falling within the scope of protection of the invention.
Claims (10)
1. A composition of a material for preventing and controlling antibiotic resistance gene pollution, which is characterized by comprising an A component, a B component and Portland cement, wherein the composition of the material comprises the following components:
(I) And (3) a component A: clay, feSO 4 ·7H 2 O、KMnO 4 ；
(II) component B: water-retaining agent and quaternary ammonium salt;
(III) portland cement;
wherein the clay and the FeSO 4 ·7H 2 O, the KMnO 4 The content weight ratio of the water-retaining agent to the quaternary ammonium salt is 10-50:3-15:2-10:10-30:2-5;
the weight content ratio of the component A, the component B and the Portland cement is 5-10:3-6:0.5-1;
in the component A, the clay is selected from any one of attapulgite, bentonite or diatomite, and the average volume particle diameter is not more than 150 mu m.
2. The composition according to claim 1, wherein in the B component, the water-retaining agent is selected from at least one of cationic polyacrylamide and starch graft type crosslinks thereof, sodium polyacrylate and starch graft type crosslinks thereof, potassium polyacrylate and starch graft type crosslinks thereof; and/or
In the component B, the quaternary ammonium salt is at least one selected from dodecyl dimethyl benzyl ammonium chloride, dodecyl trimethyl ammonium chloride, dodecyl dimethyl benzyl ammonium bromide, tetradecyl dimethyl benzyl ammonium chloride, cyano quaternary ammonium salt, double C8 alkyl quaternary ammonium bromide, polyazacyclic quaternary ammonium salt, pyridine derivatives, polyquaternary ammonium salt and double quaternary ammonium salt.
3. The composition according to claim 1 or 2, wherein the portland cement is selected from at least one of early strength portland cements having a strength of the order PO32.5R-PO42.5R.
4. The composition according to claim 1 or 2, wherein the composition further comprises water.
5. A method for preparing a material for resistance to genetic contamination by a controlled antibiotic, the method being carried out using a substance in a composition according to any one of claims 1 to 4, comprising:
(1) Preparing a material A: clay, feSO in the presence of water I 4 ·7H 2 O、KMnO 4 After first mixing, regulating the pH value to 7-8, then carrying out first contact reaction, and obtaining a material A after separation, washing and drying;
(2) Preparing a material B: mixing the aqueous solution of the quaternary ammonium salt with a water-retaining agent to perform a second contact reaction, and separating to obtain a material B;
(3) Preparing a resistance and antibiotic resistance gene pollution material: carrying out second mixing on the material A and Portland cement and water III to obtain a mixture I; wherein the amount of water II is controlled such that the water content of the mixture I is 60t%;
rolling and granulating the mixture I and the material B, and drying to obtain the resistance gene pollution material;
wherein the clay and the FeSO 4 ·7H 2 O, the KMnO 4 The weight ratio of the water-retaining agent to the quaternary ammonium salt is 10-50:3-15:2-10:10-30:2-5;
the weight ratio of the material A to the material B to the Portland cement is 5-10:3-6:0.5-1.
6. The method according to claim 5, wherein in step (2), the concentration of the quaternary ammonium salt in the aqueous solution of the quaternary ammonium salt is 2 to 5wt%.
7. The method according to claim 5 or 6, wherein in step (1), the conditions of the first mixing at least satisfy: stirring at 300-400rpm for 10-15min; and/or
In step (1), the conditions of the first contact reaction at least satisfy: stirring at 22-28deg.C at 300-400rpm for 6-12 hr; and/or
In step (1), the drying conditions at least satisfy: the temperature is 100-120 ℃ and the time is 4-6h.
8. The method according to claim 5 or 6, wherein in step (2), the conditions of the second contact reaction at least satisfy: stirring at 22-28deg.C at 300-400rpm for 4-8 hr; and/or
In step (3), the conditions of the second mixing at least satisfy: the stirring is carried out under the condition of 300-400rpm for 0.5-1h.
9. A resistance-controlled antibiotic-resistance gene contaminated material prepared by the method of any one of claims 5 to 8.
10. The use of the resistance-controlled antibiotic resistance gene pollution material of claim 9 in soil pollution control and remediation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311417225.2A CN117603697A (en) | 2023-10-30 | 2023-10-30 | Resistance-controlled antibiotic resistance gene pollution material composition, resistance-controlled pollution material, preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311417225.2A CN117603697A (en) | 2023-10-30 | 2023-10-30 | Resistance-controlled antibiotic resistance gene pollution material composition, resistance-controlled pollution material, preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117603697A true CN117603697A (en) | 2024-02-27 |
Family
ID=89945024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311417225.2A Pending CN117603697A (en) | 2023-10-30 | 2023-10-30 | Resistance-controlled antibiotic resistance gene pollution material composition, resistance-controlled pollution material, preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117603697A (en) |
-
2023
- 2023-10-30 CN CN202311417225.2A patent/CN117603697A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105859060B (en) | A kind of processing method of sewage dephosphorization denitrification | |
| CN109078972B (en) | Heavy metal contaminated soil remediation material and application method thereof | |
| AU722058B2 (en) | Compost decontamination of DDT contaminated soil | |
| CN101808757A (en) | Method for the bioremediation of soil and/or water contaminated by organic and/or inorganic compounds | |
| US20240139790A1 (en) | Composite biochar for preventing and treating heavy metal pollution and preparation method therefor | |
| CN107365227A (en) | Heavy-metal contaminated soil compost modifying agent and its modification method | |
| CN110846046A (en) | Composite repairing agent for organic contaminated soil, preparation method and application thereof | |
| WO2022126756A1 (en) | Remediation agent for petroleum-contaminated coastal soil and use thereof | |
| CN105566010A (en) | Soil amendment | |
| CN106905052A (en) | Heavy metal pollution alkaline land modifying agent and saline and alkali land improvement method comprising spent bleaching clay | |
| CN112470588B (en) | Saline-alkali soil improvement repairing method | |
| CN106433670A (en) | Preparation and application methods for amendment for heavy-metal contaminated soil | |
| CN117603697A (en) | Resistance-controlled antibiotic resistance gene pollution material composition, resistance-controlled pollution material, preparation method and application thereof | |
| CN112342031A (en) | Soil composite modifier and application thereof | |
| CN106905982A (en) | Using the heavy metal pollution alkaline land modifying agent and saline and alkali land improvement method of spent bleaching clay | |
| CN106947716A (en) | The agent of heavy metal pollution Saline-alkali Field Control and saline-alkali soil treatment method comprising spent bleaching clay | |
| US6060292A (en) | Compost decontamination of soil contaminated with methoxychlor | |
| CN112358032B (en) | Green environment-friendly slow-release oxygen increasing agent and preparation method thereof | |
| CZ62696A3 (en) | Reclaiming process of soils | |
| US6074451A (en) | Microorganism fertilizer, a method for manufacturing the microorganism fertilizer, and a method for utilizing the microorganism fertilizer | |
| US5998199A (en) | Compost decontamination of soil contaminated with TNT, HMX and RDX with aerobic and anaerobic microorganisms | |
| CN110615524A (en) | Water treatment filler easy for microbial growth | |
| CN111108835B (en) | Method for improving low organic soil | |
| KR20130071515A (en) | Composting of organic waste for water-swelling polymer by the water regulator | |
| CN106905984A (en) | Using the heavy metal pollution Saline-alkali Field Control agent of spent bleaching clay and saline-alkali soil treatment method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |