CN117589687A - Optical cavity container based on air-wrapped liquid, application and spectrum detection method - Google Patents
Optical cavity container based on air-wrapped liquid, application and spectrum detection method Download PDFInfo
- Publication number
- CN117589687A CN117589687A CN202410074326.2A CN202410074326A CN117589687A CN 117589687 A CN117589687 A CN 117589687A CN 202410074326 A CN202410074326 A CN 202410074326A CN 117589687 A CN117589687 A CN 117589687A
- Authority
- CN
- China
- Prior art keywords
- optical cavity
- cavity container
- container
- optical
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003287 optical effect Effects 0.000 title claims abstract description 163
- 239000007788 liquid Substances 0.000 title claims abstract description 64
- 238000001514 detection method Methods 0.000 title claims abstract description 24
- 238000001228 spectrum Methods 0.000 title abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 16
- 230000002209 hydrophobic effect Effects 0.000 claims description 37
- 230000001070 adhesive effect Effects 0.000 claims description 25
- 239000000853 adhesive Substances 0.000 claims description 22
- 230000000694 effects Effects 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 238000009736 wetting Methods 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 9
- 230000003595 spectral effect Effects 0.000 claims description 8
- 239000002105 nanoparticle Substances 0.000 claims description 7
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 5
- 235000012239 silicon dioxide Nutrition 0.000 claims description 5
- -1 polytetrafluoroethylene Polymers 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 238000012360 testing method Methods 0.000 claims description 4
- KXGFMDJXCMQABM-UHFFFAOYSA-N 2-methoxy-6-methylphenol Chemical compound [CH]OC1=CC=CC([CH])=C1O KXGFMDJXCMQABM-UHFFFAOYSA-N 0.000 claims description 3
- 238000001237 Raman spectrum Methods 0.000 claims description 3
- 238000002083 X-ray spectrum Methods 0.000 claims description 3
- 239000004840 adhesive resin Substances 0.000 claims description 3
- 229920006223 adhesive resin Polymers 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 229920001971 elastomer Polymers 0.000 claims description 3
- 238000002189 fluorescence spectrum Methods 0.000 claims description 3
- 229920001568 phenolic resin Polymers 0.000 claims description 3
- 239000005011 phenolic resin Substances 0.000 claims description 3
- 238000001259 photo etching Methods 0.000 claims description 3
- 229920000058 polyacrylate Polymers 0.000 claims description 3
- 239000004814 polyurethane Substances 0.000 claims description 3
- 229920002635 polyurethane Polymers 0.000 claims description 3
- 238000004528 spin coating Methods 0.000 claims description 3
- 238000002211 ultraviolet spectrum Methods 0.000 claims description 3
- 238000007740 vapor deposition Methods 0.000 claims description 3
- 239000005543 nano-size silicon particle Substances 0.000 claims description 2
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 abstract description 8
- 238000004458 analytical method Methods 0.000 abstract description 6
- 230000005540 biological transmission Effects 0.000 abstract description 3
- 230000005284 excitation Effects 0.000 description 10
- 239000004033 plastic Substances 0.000 description 10
- 229920003023 plastic Polymers 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000001069 Raman spectroscopy Methods 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 238000004611 spectroscopical analysis Methods 0.000 description 7
- 239000011347 resin Substances 0.000 description 5
- 229920005989 resin Polymers 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000002103 nanocoating Substances 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000036571 hydration Effects 0.000 description 3
- 238000006703 hydration reaction Methods 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- 239000010453 quartz Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 3
- 238000010146 3D printing Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229920000122 acrylonitrile butadiene styrene Polymers 0.000 description 2
- VYXSBFYARXAAKO-WTKGSRSZSA-N chembl402140 Chemical compound Cl.C1=2C=C(C)C(NCC)=CC=2OC2=C\C(=N/CC)C(C)=CC2=C1C1=CC=CC=C1C(=O)OCC VYXSBFYARXAAKO-WTKGSRSZSA-N 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 229940043267 rhodamine b Drugs 0.000 description 2
- 238000010183 spectrum analysis Methods 0.000 description 2
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- KVIPHDKUOLVVQN-UHFFFAOYSA-N ethene;hydrate Chemical compound O.C=C KVIPHDKUOLVVQN-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- DIVDFFZHCJEHGG-UHFFFAOYSA-N oxidopamine Chemical compound NCCC1=CC(O)=C(O)C=C1O DIVDFFZHCJEHGG-UHFFFAOYSA-N 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000003075 superhydrophobic effect Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- JLZUZNKTTIRERF-UHFFFAOYSA-N tetraphenylethylene Chemical group C1=CC=CC=C1C(C=1C=CC=CC=1)=C(C=1C=CC=CC=1)C1=CC=CC=C1 JLZUZNKTTIRERF-UHFFFAOYSA-N 0.000 description 1
Abstract
The invention belongs to the technical field of instrument analysis, and particularly relates to an optical cavity container based on air-wrapped liquid, application and a spectrum detection method. The optical cavity container is based on an air-wrapped liquid/air interface and an inner wall material structure of the optical cavity container, so that a liquid sample in the optical cavity container becomes an optical resonant cavity similar to a lens and used for concentrating and amplifying light. According to the invention, through the inner wall material structure of the optical cavity container, an air layer with low refractive index can be efficiently formed between the liquid and the optical cavity container, and an air/liquid interface with abrupt refractive index is obtained, so that the sample liquid becomes an optical resonant cavity similar to a lens and used for concentrating amplified light, and efficient transmission of a spectrum signal generated by the liquid to a light detector is realized. The optical cavity container has the advantages of good self-cleaning capability, reusability, good stability, suitability for various spectrum detection methods and the like.
Description
Technical Field
The invention belongs to the technical field of instrument analysis, and particularly relates to an optical cavity container with air almost completely wrapping liquid.
Background
A method of qualitatively and quantitatively analyzing a substance by using the spectrum of the substance is called spectroscopic analysis. In most cases, the object of the spectrum analysis is a liquid sample, and the information of the substance, structure, concentration and the like of the sample to be detected can be clearly obtained by analyzing the absorbance, optical rotation, fluorescence, phosphorescence, raman, terahertz and other spectrums of the liquid sample. While the container carrying the liquid to be measured is generally referred to as a cuvette and in optical analysis is generally referred to as a cuvette. According to the application, the color matching device can be divided into an ultraviolet cuvette, a visible light cuvette, an infrared cuvette, a fluorescent cuvette and the like. According to the materials, the materials can be classified into glass cuvettes, quartz cuvettes, plastic cuvettes and the like.
Along with the development of society, a sample pool is also continuously developed into new categories according to the needs of the times. The innovations for sample cells are still primarily improved and optimized from the standpoint of self-material, geometry, size, etc. Although various liquid sample cells for spectroscopic analysis have been developed in the prior art, the sample cells have been mainly responsible for the container function since the invention as an indispensable key component in spectroscopic analysis. During excitation, generation, and transmission of the spectroscopic analysis signal, not much is contributed to the spectroscopic signal control. In some studies, researchers have conducted some signal enhancement studies on sample cells in order to boost the contribution of the Chi Duiguang spectral signal of the sample. For example, in the literature, "drive up to Liu Guxiang, li Zhengang, et al," study [ J ]. Spectrometry and Spectrometry based on liquid Raman Spectroscopy enhancement method for novel sample cells, 2023, 43 (3): 712-717 ], the spectral signal is enhanced from a geometric optics perspective by changing the material of the sample cell to a mirror capable of reflecting light, and changing the bottom of the circular sample cell to a concave mirror with light reflection. However, this method has limited spectral signal enhancement effect and has high requirements on the flatness, material and arrangement position of the mirror surface. And the optical mirror surface with high reflection has higher cost and poorer universality, and is difficult to popularize on a large scale.
In general, there are few prior art liquid sample reservoirs for optical enhancement, and there is a need in the art to develop new liquid optical cavity containers with optical enhancement.
Disclosure of Invention
The invention aims at the problems in the prior art and provides an air-wrapped optical cavity container so as to enhance a spectrum signal during spectrum analysis.
The invention is based on an optical cavity container of air-wrapped liquid, and the optical cavity container is based on an air-wrapped liquid/air interface and an inner wall material structure of the optical cavity container, so that a liquid sample in the optical cavity container becomes an optical resonant cavity, and the optical resonant cavity has the same function of concentrating and amplifying light as a lens. Wherein the material of the optical cavity container is at least one selected from metal, glass, quartz, PMMA plastic, PS plastic, PET plastic, PTFE plastic, ABS plastic or 3D printing resin.
Preferably, the inner surface of the optical cavity container is provided with a super-wetting hydrophobic structure, and when the optical cavity container is used for optical signal detection, an air layer wrapping the liquid sample is formed between the liquid sample and the inner wall of the container, and an air/liquid interface with abrupt refractive index changes is obtained.
Preferably, the thickness of the super-wetted hydrophobic structure is 10-500 μm.
Preferably, the super-wetting hydrophobic structure is prepared by adopting a method of spraying, coating, spin coating, photoetching, nanoimprint or vapor deposition.
Preferably, the super-wetting hydrophobic structure is prepared by adhering hydrophobic nano-particles on the inner surface of the optical cavity container through an adhesive.
Preferably, the hydrophobic nanoparticle is selected from at least one of hydrophobic nanosilica or hydrophobic nanosilica; and/or the adhesive is at least one of polyacrylate adhesive, polyurethane adhesive, polytetrafluoroethylene adhesive, rubber adhesive or phenolic resin adhesive.
Preferably, the optical cavity container is a square optical cavity container, a round optical cavity container, an annular optical cavity container, a U-shaped optical cavity container, a Y-shaped optical cavity container, a multi-channel optical cavity container, a flat bottom configuration optical cavity container, an elliptic bottom configuration optical cavity container or a conical sharp bottom configuration optical cavity container.
Preferably, the optical cavity container is used for detection of fluorescence spectrum, raman spectrum, ultraviolet spectrum or X-ray spectrum.
The invention also provides the application of the optical cavity container for air-wrapped liquid in spectrum detection.
The invention also provides a spectrum detection method, which carries out spectrum detection after the optical cavity container carries the liquid sample.
The inner surface of the optical cavity container is of a super-infiltration hydrophobic structure, the super-infiltration hydrophobic structure can efficiently form an air layer with low refractive index between liquid and the optical cavity container, the liquid sample is almost completely wrapped by air, and an air/liquid interface with abrupt refractive index is obtained. The sample liquid is an optical resonant cavity similar to a lens and used for concentrating amplified light based on air layer wrapping of an optical cavity container and design of liquid geometric structure, so that efficient transmission of spectrum signals generated by the liquid to a light detector is realized. In addition, the internal surface structure and materials of the container can influence the photophysical properties of the solute by changing the arrangement state of water molecules on the surface of the liquid sample and the hydration shell structure formed by water and the solute. For example, in a fluorescence mode, the absolute fluorescence quantum yield of the solute in the liquid sample can be significantly improved and a significant fluorescence enhancement can be exhibited. Whereas the light detector receives only a small signal when the air without the container wraps around the liquid.
In addition, the optical cavity container has the advantages of good self-cleaning capability, reusability, good stability, suitability for various spectrum detection methods and the like. Therefore, the invention has good application prospect.
It should be apparent that, in light of the foregoing, various modifications, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
The above-described aspects of the present invention will be described in further detail below with reference to specific embodiments in the form of examples. It should not be understood that the scope of the above subject matter of the present invention is limited to the following examples only. All techniques implemented based on the above description of the invention are within the scope of the invention.
Drawings
Fig. 1 is an optical enhancement schematic diagram of the optical cavity container of embodiment 1, wherein a is a flow chart of the use of the optical cavity container, b is a schematic diagram of the optical enhancement principle of the optical cavity container, and c is a schematic diagram of the optical cavity container in the prior art.
Fig. 2 is a schematic diagram and a characterization result of a super-wetted hydrophobic structure of the optical cavity container in example 1, wherein a is a structural perspective view of the optical cavity container, b is an enlarged view of inner wall details of the super-wetted hydrophobic structure, c is a scanning electron microscope image of inner wall details of the super-wetted hydrophobic structure, and d is a water contact angle test image of the super-wetted hydrophobic structure.
FIG. 3 is a graph showing the fluorescence signal enhancement effect of the optical cavity container of example 1 in a fluorescence imaging system.
Fig. 4 is a graph showing the fluorescence signal enhancement effect of the optical cavity container of example 1 in a multifunctional microplate reader.
Fig. 5 is a graph showing the raman spectral signal enhancement effect of the optical cavity container of example 1 in a raman spectrometer.
Fig. 6 is a graph showing the ultraviolet-visible spectrum signal enhancement effect of the optical cavity container of example 1 in a multifunctional microplate reader.
Fig. 7 is a comparison of self-cleaning performance of the optical cavity container of example 1 and a commercial plastic optical cavity container.
Detailed Description
The reagents and raw materials used in the following examples were commercially available, which were not specifically described.
Example 1 air-wrap liquid based optical cavity Container
The invention is based on an optical cavity container of air-wrapped liquid, and the optical cavity container is based on an air-wrapped liquid/air interface and an inner wall material structure of the optical cavity container, so that a liquid sample in the optical cavity container becomes an optical resonant cavity, and the optical resonant cavity has the function of concentrating and amplifying light like a lens. Wherein the material of the optical cavity container is at least one selected from metal, glass, quartz, PMMA plastic, PS plastic, PET plastic, PTFE plastic, ABS plastic or 3D printing resin.
The inner surface of the optical cavity container is provided with a super-wetting hydrophobic structure, the thickness of the super-wetting hydrophobic structure is 10-500 mu m, and the super-wetting hydrophobic structure is prepared by adopting a spraying, coating, spin coating, photoetching, nanoimprint or vapor deposition method. When the optical cavity container is used for optical signal detection, an air layer which almost completely wraps the liquid sample is formed between the liquid sample and the inner wall of the container, and an air/liquid interface with abrupt refractive index changes is obtained.
The super-wetting hydrophobic structure is prepared by adhering hydrophobic nano particles on the inner surface of the optical cavity container through an adhesive. Wherein,
the hydrophobic nano particles are selected from at least one of hydrophobic nano silicon dioxide or hydrophobic nano titanium dioxide; and/or the adhesive is at least one of polyacrylate adhesive, polyurethane adhesive, polytetrafluoroethylene adhesive, rubber adhesive or phenolic resin adhesive.
The optical cavity container is a square optical cavity container, a round optical cavity container, an annular optical cavity container, a U-shaped optical cavity container, a Y-shaped optical cavity container, a multi-channel optical cavity container, a flat bottom configuration optical cavity container, an elliptic bottom configuration optical cavity container or a conical sharp bottom configuration optical cavity container.
The optical cavity container is used for detecting fluorescence spectrum, raman spectrum, ultraviolet spectrum or X-ray spectrum.
The method and principle of using the optical cavity container provided in this embodiment are shown in fig. 1, and the structure and characterization result are shown in fig. 2. Specifically, the cell body of the optical cavity container can be any optical cavity container in the prior art, and in the spectrum test of this embodiment, if the excitation light is from top to bottom and the signal is received from top, the conical pointed bottom optical cavity container is selected; if the excitation light is from bottom to top and the signal is received at the upper part, a conical pointed-bottom-shaped optical cavity container with a small hole (aperture 1.5 and mm and through arrangement) at the center of the bottom and capable of avoiding liquid leakage due to the surface tension of liquid is selected; the material of the optical cavity container is selected to be photosensitive resin (transparent photosensitive resin special for Dongguan Jun Yi SLA).
The super-wetted hydrophobic structure is prepared by spraying in this example, specifically, a hydrophobic silica nanoparticle solution (model: 260, solid content 20 wt% of Aba Ding Shiji company) containing an adhesive is sprayed 3 times (100 μl of each spray amount) into an optical cavity container (inner surface area of 2.5 cm) 2 ) Standing until the nano coating is completely cured, and preserving for standby (the thickness of the cured nano coating is 50 μm). The adhesive can be selected from various existing high-molecular adhesive solvents with strong adhesive force, and the embodiment is specifically selected from polypropylene resin. The characterization results of the nano coating (super-wetting hydrophobic structure) are shown in fig. 2c and 2d, and the results show that the prepared nano coating has a rough surface and good super-hydrophobic performance.
When the optical cavity container of the embodiment is used, due to the super-infiltration performance of the inner surface of the optical cavity container, a thin air layer can be formed between the liquid sample and the optical cavity container, and the interface and the geometrical constraint of the optical cavity container are utilized to realize the almost complete package of the air on the liquid sample. That is to say that the liquid sample actually has a very small area of direct contact with the optical cavity vessel, similar to the pattern of contact of a lotus leaf with a bead, which pattern is referred to in the theory of surface wettability as the Cassie-Baxter state. In a conventional optical cavity vessel, the spectroscopic analysis process is as follows: excitation light enters the liquid sample to interact with the liquid sample and generate a spectrum signal, and then the spectrum signal passes through the liquid phase directly through the optical cavity container and enters the optical detector. In the optical cavity container of this embodiment, the spectral signal passes through the air layer with low refractive index after passing through the liquid phase, and is refracted and reflected repeatedly at the interface from high refractive index to low refractive index, and the design of the liquid geometry is combined to make the sample liquid become an optical resonant cavity similar to a lens for concentrating amplified light. And then into an optical detector. Therefore, the invention realizes the obvious enhancement and regulation of the optical signal by the influence of the spontaneously formed optical cavity and the liquid/air interface on the water molecule arrangement and the hydration shell structure based on the air layer and the liquid drop geometric configuration of the optical cavity container.
Example 2 Spectrum detection method
The present embodiment provides a spectroscopic detection method for performing spectroscopic detection using the optical cavity container provided in embodiment 1 carrying a liquid sample. Other supplements and devices for spectroscopic detection are the same as in the prior art.
In order to compare the optical enhancement and self-cleaning capabilities, this example provided, in addition to the detection results of the optical cavity containers of example 1 as an experimental group, an optical cavity container using an existing optical cavity container (i.e., an optical cavity container in which a super-wetted hydrophobic structure was not prepared by the method of example 1) as a control group.
FIG. 3 provides a comparison of fluorescence signal enhancement effects in a Invitrogen iBright 1500 fluorescence imaging system with excitation light from top to bottom, signal received on top, detection samples water, rhodamine 6G (10. Mu.M) and rhodamine B (10. Mu.M). It can be seen from the figure that the optical cavity container provided in example 1 has a remarkable enhancement effect on fluorescence intensity.
FIG. 4 provides a comparison of fluorescence signal enhancement effects in a PerkinElmer EnVision multifunctional microplate reader, with excitation light from top to bottom, signal received on top, and sample tested as water and tetraphenyl ethylene (2.5. Mu.M) fluorochrome. It can be seen from the figure that the optical cavity container provided in example 1 has a remarkable enhancement effect on fluorescence intensity.
Fig. 5 provides a graph of raman spectral signal enhancement effect in a Horiba HR Evolution raman spectrometer with excitation light from top to bottom, signal received on top, and rhodamine 6G (0.5 μm) as the test sample. It can be seen from the figure that the optical cavity container provided in example 1 has a significant enhancement effect on raman signal intensity.
FIG. 6 provides a graph of the enhancement effect of the ultraviolet-visible spectrum signal in a PerkinElmer EnVision multifunctional microplate reader, wherein the excitation light is from bottom to top, the signal is received from top, and the detection sample is rhodamine B (10 mu M). There are two kinds of optical cavity containers for the control group, wherein the control group 1 is a conventional commercial optical cavity container (Corning 42592), and the control group 2 is a commercial ultraviolet analysis optical cavity container (Corning 3635). As can be seen from fig. 6, the optical cavity container of example 1 has a significant uv-vis enhancement capability, and in the uv band less than 290, 290 nm, the analysis capability has been completely lost due to the strong absorption of uv by the conventional PS cuvette, while the air cuvette is still able to be used normally, comparable to a commercial uv optical cavity container. The bottom of the optical cavity container of example 1 has a small hole with a diameter of 1.5 and mm, the background signal is lower based on the super-wetted hydrophobic structure and geometric constraint (because of air wrapping and no absorption of excitation light by other materials), and based on the optical enhancement principle, the optical cavity container has very low background noise and obvious ultraviolet-visible spectrum enhancement capability in the detection mode of the excitation light from bottom to top, and the ultraviolet analysis window range can be widened from 290 nm to 230 nm.
In order to further verify that the optical cavity containers of the present invention have good self-cleaning properties, the following experiments were performed: 50. Mu.L of BODIPY fluorescent dye (100 nM) was added to two different optical chamber containers, and after standing for 1 minute, the solutions in the two optical chamber containers were poured out, and then the fluorescent signal of the residue in the optical chamber containers was measured. The results are shown in fig. 7, where the nanointerface air-wrap optical cavity container has significant self-cleaning capability relative to commercially available commercial optical cavity containers, yet retains background interference from the substrate over 100 cycles. This shows that the self-cleaning performance of the super-wetted hydrophobic structure prepared in the optical cavity container of this example 1 is significantly improved.
It can be seen from the above embodiments that the present invention provides a new optical cavity container that by design of the container structure and materials forms a nearly fully encapsulated air layer between the liquid sample and the inner wall of the container. On one hand, the invention makes light have multiple refraction and reflection on the water/air interface, and then combines the design of the liquid geometry to make the sample liquid become an optical resonant cavity similar to a lens for concentrating and amplifying light; on the other hand, the photophysical process of the solute is affected by the molecular arrangement state of water on the three-phase contact interface of the water/air/container hydrophobic interface and the difference of the hydration shell structure formed by water and the solute. A significant enhancement of the spectral signal is finally achieved. In addition, the liquid optical cavity container has the advantages of good self-cleaning capability, reusability, good stability, suitability for various spectrum detection methods and the like. Therefore, the invention has good application prospect in spectrum detection.
Claims (10)
1. Optical cavity container based on air parcel liquid, its characterized in that: the optical cavity container is based on an air-wrapped liquid/air interface and an inner wall material structure of the optical cavity container, so that a liquid sample in the optical cavity container becomes an optical resonant cavity, and the optical resonant cavity has the effect of amplifying light intensively.
2. The optical cavity receptacle of claim 1, wherein: the inner surface of the optical cavity container is provided with a super-wetting hydrophobic structure, and when the optical cavity container is used for optical signal detection, an air layer wrapping the liquid sample is formed between the liquid sample and the inner wall of the container, and an air/liquid interface with abrupt refractive index changes is obtained.
3. An optical cavity receptacle according to claim 2, wherein: the thickness of the super-wetting hydrophobic structure is 10-500 mu m.
4. An optical cavity receptacle according to claim 2, wherein: the super-wetting hydrophobic structure is prepared by adopting methods of spraying, coating, spin coating, photoetching, nanoimprint or vapor deposition.
5. An optical cavity receptacle according to claim 2, wherein: the super-wetting hydrophobic structure is prepared by adhering hydrophobic nano particles on the inner surface of the optical cavity container through an adhesive.
6. The optical cavity receptacle of claim 5, wherein: the hydrophobic nano particles are selected from at least one of hydrophobic nano silicon dioxide or hydrophobic nano titanium dioxide;
and/or the adhesive is at least one of polyacrylate adhesive, polyurethane adhesive, polytetrafluoroethylene adhesive, rubber adhesive or phenolic resin adhesive.
7. The optical cavity receptacle of claim 1, wherein: the optical cavity container is a square optical cavity container, a round optical cavity container, an annular optical cavity container, a U-shaped optical cavity container, a Y-shaped optical cavity container, a multi-channel optical cavity container, a flat bottom configuration optical cavity container, an elliptic bottom configuration optical cavity container or a conical sharp bottom configuration optical cavity container.
8. The optical cavity receptacle of claim 1, wherein: the optical cavity container is used for detecting fluorescence spectrum, raman spectrum, ultraviolet spectrum or X-ray spectrum.
9. Use of the optical cavity vessel according to any of claims 1-8 in spectroscopic detection.
10. A method of spectral detection, characterized by: spectroscopic testing of a liquid sample carried by an optical cavity container according to any of claims 1-8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410074326.2A CN117589687B (en) | 2024-01-18 | 2024-01-18 | Optical cavity container based on air-wrapped liquid, application and spectrum detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410074326.2A CN117589687B (en) | 2024-01-18 | 2024-01-18 | Optical cavity container based on air-wrapped liquid, application and spectrum detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117589687A true CN117589687A (en) | 2024-02-23 |
| CN117589687B CN117589687B (en) | 2024-04-09 |
Family
ID=89916971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410074326.2A Active CN117589687B (en) | 2024-01-18 | 2024-01-18 | Optical cavity container based on air-wrapped liquid, application and spectrum detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117589687B (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6694067B1 (en) * | 2001-01-05 | 2004-02-17 | Los Gatos Research | Cavity enhanced fiber optic and waveguide chemical sensor |
| US20090002700A1 (en) * | 2007-06-29 | 2009-01-01 | Intel Corporation | Solution sample plate with wells designed for improved raman scattering signal detection efficiency |
| US20090117384A1 (en) * | 2007-11-07 | 2009-05-07 | Brookhaven Science Associates, Llc | Titania Nanocavities and Method of Making |
| CN103869021A (en) * | 2014-03-28 | 2014-06-18 | 苏州大学 | High performance liquid chromatography detection method and device based on surface enhanced Raman spectroscopy |
| CN104458583A (en) * | 2014-12-12 | 2015-03-25 | 东北石油大学 | Optical cavity for testing sewage transmission spectrum |
| US20150276481A1 (en) * | 2012-09-25 | 2015-10-01 | The Penn State Research Foundation | Resonator Enhanced Raman Spectroscopy |
| CN107110774A (en) * | 2014-12-23 | 2017-08-29 | 马克斯-普朗克科学促进学会 | Method and instrument for measure spectrum sample response |
| US20170315052A1 (en) * | 2014-11-06 | 2017-11-02 | Board Of Regents, The University Of Texas System | Systems and methods for performing cavity-enhanced absorption spectroscopy |
| CN207423808U (en) * | 2017-11-10 | 2018-05-29 | 苏州贝康医疗器械有限公司 | A kind of raman spectral signal strengthening system suitable for atomic quantity of fluid |
| CN109752363A (en) * | 2019-02-28 | 2019-05-14 | 江苏大学 | The remaining Portable Raman optical spectrum detection method of pesticide in a kind of instant tea powder |
| CN110196245A (en) * | 2018-02-26 | 2019-09-03 | 成都艾立本科技有限公司 | A kind of laser induced breakdown spectroscopy detection system |
| US20220244226A1 (en) * | 2019-02-21 | 2022-08-04 | Institut für Nanophotonik Goettingen e.V. | Method and Apparatus for Identifying Volatile Substances Using Resonator-Amplified Raman Spectroscopy Under Reduced Pressure |
| US20220334063A1 (en) * | 2019-08-22 | 2022-10-20 | Cape Breton University | Methods of modifying a liquid sample containing an analyte so as to increasesers signal intensity of the analyte, as well as a probe for remote sensing of an analyte using sers |
| CN116482075A (en) * | 2023-05-23 | 2023-07-25 | 西安邮电大学 | Preparation method of three-dimensional hydrophobic gold-silver alloy nano substrate with Raman activity |
-
2024
- 2024-01-18 CN CN202410074326.2A patent/CN117589687B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6694067B1 (en) * | 2001-01-05 | 2004-02-17 | Los Gatos Research | Cavity enhanced fiber optic and waveguide chemical sensor |
| US20090002700A1 (en) * | 2007-06-29 | 2009-01-01 | Intel Corporation | Solution sample plate with wells designed for improved raman scattering signal detection efficiency |
| US20090117384A1 (en) * | 2007-11-07 | 2009-05-07 | Brookhaven Science Associates, Llc | Titania Nanocavities and Method of Making |
| US20150276481A1 (en) * | 2012-09-25 | 2015-10-01 | The Penn State Research Foundation | Resonator Enhanced Raman Spectroscopy |
| CN103869021A (en) * | 2014-03-28 | 2014-06-18 | 苏州大学 | High performance liquid chromatography detection method and device based on surface enhanced Raman spectroscopy |
| US20170315052A1 (en) * | 2014-11-06 | 2017-11-02 | Board Of Regents, The University Of Texas System | Systems and methods for performing cavity-enhanced absorption spectroscopy |
| CN104458583A (en) * | 2014-12-12 | 2015-03-25 | 东北石油大学 | Optical cavity for testing sewage transmission spectrum |
| CN107110774A (en) * | 2014-12-23 | 2017-08-29 | 马克斯-普朗克科学促进学会 | Method and instrument for measure spectrum sample response |
| CN207423808U (en) * | 2017-11-10 | 2018-05-29 | 苏州贝康医疗器械有限公司 | A kind of raman spectral signal strengthening system suitable for atomic quantity of fluid |
| CN110196245A (en) * | 2018-02-26 | 2019-09-03 | 成都艾立本科技有限公司 | A kind of laser induced breakdown spectroscopy detection system |
| US20220244226A1 (en) * | 2019-02-21 | 2022-08-04 | Institut für Nanophotonik Goettingen e.V. | Method and Apparatus for Identifying Volatile Substances Using Resonator-Amplified Raman Spectroscopy Under Reduced Pressure |
| CN109752363A (en) * | 2019-02-28 | 2019-05-14 | 江苏大学 | The remaining Portable Raman optical spectrum detection method of pesticide in a kind of instant tea powder |
| US20220334063A1 (en) * | 2019-08-22 | 2022-10-20 | Cape Breton University | Methods of modifying a liquid sample containing an analyte so as to increasesers signal intensity of the analyte, as well as a probe for remote sensing of an analyte using sers |
| CN116482075A (en) * | 2023-05-23 | 2023-07-25 | 西安邮电大学 | Preparation method of three-dimensional hydrophobic gold-silver alloy nano substrate with Raman activity |
Non-Patent Citations (3)
| Title |
|---|
| ZHANG, PF等: "Opto-Microfluidic Fabry-Perot Sensor with Extended Air Cavity and Enhanced Pressure Sensitivity", 《MICROMACHINES》, vol. 13, no. 1, 31 January 2022 (2022-01-31), pages 1 - 9 * |
| 万福等: "气体拉曼传感增强技术研究进展与趋势", 《光谱学与光谱分析》, vol. 42, no. 11, 31 December 2022 (2022-12-31), pages 3345 - 335 * |
| 赵伟东: "基于有序阵列结构的SERS基底制备与性能研究", 《中国博士学位论文全文数据库工程科技Ⅰ辑》, no. 01, 15 January 2021 (2021-01-15) * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117589687B (en) | 2024-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ahmadivand et al. | Terahertz plasmonics: The rise of toroidal metadevices towards immunobiosensings | |
| Mekonnen et al. | Dielectric nanosheet modified plasmonic-paper as highly sensitive and stable SERS substrate and its application for pesticides detection | |
| Di Meo et al. | Metasurface based on cross-shaped plasmonic nanoantennas as chemical sensor for surface-enhanced infrared absorption spectroscopy | |
| Anderson | Nonplasmonic surface enhanced Raman spectroscopy using silica microspheres | |
| JP5182900B2 (en) | Sample detection sensor and sample detection method | |
| Pelletier et al. | Spectroscopic theory for chemical imaging | |
| Heidarzadeh | Highly sensitive plasmonic sensor based on ring shape nanoparticles for the detection of ethanol and D-glucose concentration | |
| Shih et al. | MIR plasmonic liquid sensing in nano-metric space driven by capillary force | |
| US8649000B1 (en) | Whispering gallery optical resonator spectroscopic probe and method | |
| CN111337445B (en) | Dielectric super surface based on angle scanning enhanced infrared spectrum absorption | |
| CN101324528B (en) | Thin film with local field enhancement function and preparing method thereof | |
| CN117589687B (en) | Optical cavity container based on air-wrapped liquid, application and spectrum detection method | |
| Culler et al. | The use of infrared methods to study polymer interfaces | |
| CN109060768A (en) | A method of based on Surface enhanced Raman spectroscopy trace detection erythrosine concentration | |
| CN112934281A (en) | Artificial surface plasmon micro-fluidic detection chip structure based on periodic structure and preparation and detection methods thereof | |
| Li et al. | Antenna array-enhanced attenuated total reflection IR analysis in an aqueous solution | |
| Kasuya et al. | Anisotropic light absorption by localized surface plasmon resonance in a thin film of gold nanoparticles studied by visible multiple-angle incidence resolution spectrometry | |
| Ikehata et al. | High sensitive detection of near-infrared absorption by surface plasmon resonance | |
| Guzonas et al. | Infrared spectra of monolayers adsorbed on mica | |
| CN106198459B (en) | Bioanalysis sensing device based on Nanosurface plasma resonance sensor | |
| Danz et al. | Biosensing platform combining label-free and labelled analysis using Bloch surface waves | |
| CN114295557A (en) | Surface plasma resonance sensing chip and perfluorinated compound detection method | |
| ALDABİB et al. | The effects of concentration based on the absorbance form the Ultraviolet–Visible (UV-VIS) spectroscopy analysis | |
| Plowman et al. | Surface sensitivity of SiON integrated optical waveguides (IOWs) examined by IOW-attenuated total reflection spectrometry and IOW-Raman spectroscopy | |
| Chen et al. | Identification and Quantitative Detection of Pesticide Residues Using Overcoupled Metamaterial Absorber |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |