CN117551573A - Microbial preparation method for ecological breeding - Google Patents
Microbial preparation method for ecological breeding Download PDFInfo
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- CN117551573A CN117551573A CN202311510594.6A CN202311510594A CN117551573A CN 117551573 A CN117551573 A CN 117551573A CN 202311510594 A CN202311510594 A CN 202311510594A CN 117551573 A CN117551573 A CN 117551573A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 60
- 230000000813 microbial effect Effects 0.000 title claims abstract description 59
- 238000009395 breeding Methods 0.000 title claims abstract description 25
- 230000001488 breeding effect Effects 0.000 title claims abstract description 25
- 244000005700 microbiome Species 0.000 claims abstract description 81
- 238000012258 culturing Methods 0.000 claims abstract description 18
- 238000012216 screening Methods 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 16
- 238000010790 dilution Methods 0.000 claims description 12
- 239000012895 dilution Substances 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 12
- 238000009635 antibiotic susceptibility testing Methods 0.000 claims description 9
- 238000002955 isolation Methods 0.000 claims description 9
- 230000000877 morphologic effect Effects 0.000 claims description 9
- 241000186361 Actinobacteria <class> Species 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 238000005138 cryopreservation Methods 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 6
- 239000004310 lactic acid Substances 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 230000000243 photosynthetic effect Effects 0.000 claims description 6
- 239000000523 sample Substances 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000006041 probiotic Substances 0.000 claims description 5
- 235000018291 probiotics Nutrition 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 4
- 241000295146 Gallionellaceae Species 0.000 claims description 3
- 238000010222 PCR analysis Methods 0.000 claims description 3
- 238000012300 Sequence Analysis Methods 0.000 claims description 3
- 239000003242 anti bacterial agent Substances 0.000 claims description 3
- 229940088710 antibiotic agent Drugs 0.000 claims description 3
- 238000009360 aquaculture Methods 0.000 claims description 3
- 244000144974 aquaculture Species 0.000 claims description 3
- 238000002306 biochemical method Methods 0.000 claims description 3
- 239000012470 diluted sample Substances 0.000 claims description 3
- 238000001952 enzyme assay Methods 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims description 3
- 238000012252 genetic analysis Methods 0.000 claims description 3
- 238000013095 identification testing Methods 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 230000007774 longterm Effects 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 230000002503 metabolic effect Effects 0.000 claims description 3
- 239000002207 metabolite Substances 0.000 claims description 3
- 238000001823 molecular biology technique Methods 0.000 claims description 3
- 230000001546 nitrifying effect Effects 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 241001148471 unidentified anaerobic bacterium Species 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 4
- 230000009466 transformation Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention relates to the technical field of microbial preparation, and discloses a microbial preparation method for ecological breeding, which comprises the following specific steps: selecting microorganisms, collecting and culturing the microorganisms, screening and identifying the microorganisms, preparing microbial preparations and applying the microbial preparations, selecting proper microorganism types according to the requirements of ecological breeding, culturing the collected microorganisms, screening and identifying the microorganisms, selecting strains with good characteristics, culturing and amplifying the screened strains, preparing the microbial preparations, and applying the prepared microbial preparations to the ecological breeding environment, so that the transformation function is realized, the risks of various costs are reduced, and the microbial preparations have obvious promotion effect on the cultured animals and plants.
Description
Technical Field
The invention relates to the technical field of microbial preparation, in particular to a microbial preparation method for ecological breeding.
Background
The ecological cultivation means that according to the symbiotic complementation principle among different cultivated organisms, different organisms are grown together in the same environment by utilizing a natural substance circulation system in a certain cultivation space and area and through corresponding technology and management measures, so that an ecological balance is maintained and cultivation benefits are improved.
The microorganism includes: bacteria, fungi, a large group of small protozoa, microalgae and the like, and viruses, which are tiny in individuals, various in types and closely related to human beings, and the evaluation indexes aiming at microorganisms for ecological culture mainly comprise the purity of the microbial inoculum and the quantity of the microbial inoculum in unit mass.
In the ecological cultivation, the ecological balance of the water body is required to be maintained, but when in ecological cultivation, the microorganisms possibly die in batches due to environmental or other reasons, the balance of flora in a micro-ecological system in soil or water area is broken, a carbon, hydrogen and oxygen conversion system is blocked, harmful indexes are out of standard, and the malnutrition of ecological plants is caused by lack of dissolved oxygen.
Thus, a method for the preparation of microorganisms for ecological breeding is proposed.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides a microbial preparation method for ecological breeding, which can prepare different microbial preparations for breeding different animals and plants in ecological breeding and improve ecological breeding environment.
(II) technical scheme
The invention is realized by adopting the following technical scheme: the microbial preparation method for ecological breeding comprises the following specific steps:
s1, selecting microorganisms: selecting proper microorganism types according to the requirements of ecological cultivation;
s2, collecting and culturing microorganisms: culturing microorganisms collected from natural environment;
s3, screening and identifying microorganisms: selecting a strain with good characteristics by screening and identifying microorganisms;
s4, preparing a microbial preparation: culturing and amplifying the screened strain to prepare a microbial preparation;
s5, applying a microbial preparation: and applying the prepared microbial preparation to an ecological breeding environment.
In step S1, microorganisms common in ecological breeding include probiotics, aerobic microorganisms and bad oxygen microorganisms.
Preferably, in step S1, the probiotics include photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes and filamentous bacteria, wherein the photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes and filamentous bacteria are composed of 1:2:2:1:1, and the aerobic microorganisms may be composed of nitrifying bacteria, vulcanizing bacteria and iron bacteria, and the bad-oxygen microorganisms include anaerobic bacteria and gas generating bacteria.
Preferably, in step S2, the microorganisms collected from the natural environment may be isolated and purified to obtain single strains, and the single strains may be transferred to a new culture medium using a conventional coating method or a dilution coating method to obtain pure cultures, and then the strains may be cultured to provide a culture medium and conditions for promoting the propagation and growth of the microorganisms; the method comprises the following steps of:
a. sampling: collecting a sample from a natural environment;
b. dilution: subjecting the sample to appropriate dilution to ensure isolation of individual microbial cells;
c. separating: distributing the diluted sample on a nutrient-rich culture medium, and utilizing different culture conditions such as temperature, pH value, nutrient concentration and the like to promote the growth of microbial cells;
d. purifying: by stepwise dilution and re-isolation, individual colonies are selected, each from a single microbial cell, so that a pure single strain can be obtained.
Preferably, in step S3, strains with good properties are selected by screening and identifying microorganisms, which can be evaluated by observing the morphology, physiological properties and promotion of ecological cultures, using modern molecular biology techniques and biochemical methods, including morphological identification, physiological biochemical property identification, molecular biology identification and antibiotic susceptibility testing.
Preferably, in step S3, morphological identification: observing morphological features of the microorganism, such as colony shape, size and color, and observing cell morphology and structure using a microscope; and (3) identifying physiological and biochemical characteristics: performing a series of physiological biochemical experiments, such as enzyme activity assays and metabolite tests, to determine the metabolic characteristics of the microorganism; molecular biology identification: genetic analysis of microorganisms to determine their species or strain using molecular biological techniques such as PCR and sequence analysis; antibiotic susceptibility testing: the susceptibility of microorganisms to different antibiotics is determined using an antibiotic susceptibility test to direct clinical treatment or other applications.
Preferably, in step S4, the selected strain is cultured and amplified, and prepared into a microbial preparation, which may be in the form of a liquid culture, a solid culture or a powder.
Preferably, in step S4, the microorganism preparation is amplified and prepared by culturing, and the following steps are performed:
A. culturing the amplified strain: inoculating the screened strain into a proper culture medium, and providing proper temperature, pH and nutrition conditions to promote the growth and propagation of the strain;
B. strain purification: isolation of individual colonies onto new medium until pure strains are obtained;
C. strain storage: for long-term storage and backup of strains, cryopreservation or preparation of lyophilisates is used;
D. preparing a microbial preparation: preparing into microbial preparation, and packaging the microbial preparation into proper package;
wherein, the cryopreservation is to preserve the strain at a low temperature of-80 ℃.
Preferably, in step S5, the prepared microbial preparation may be applied by being added to feed or sprayed in a body of aquaculture water.
(III) beneficial effects
Compared with the prior art, the invention provides a microbial preparation method for ecological breeding, which has the following beneficial effects:
according to the preparation method of the microorganism for ecological cultivation, the proper microorganism type is selected according to the requirements of ecological cultivation, the collected microorganism is cultivated, the microorganism is screened and identified, the strain with good characteristics is selected, the screened strain is cultivated and amplified, the microbial preparation is prepared, and the prepared microbial preparation is applied to an ecological cultivation environment, so that the transformation function is realized, the risks of various costs are reduced, and the microbial preparation has an obvious promotion effect on cultivated animals and plants.
Drawings
Fig. 1 is an overall construction diagram of the present invention.
FIG. 2 is a flow chart of the collection and cultivation of microorganisms in the present invention.
FIG. 3 is a partial construction diagram of a microbial preparation prepared by culturing in the present invention.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, on the premise of no conflict, the following embodiments or technical features may be arbitrarily combined to form new embodiments.
Example 1:
referring to fig. 1-3, the method for preparing a microorganism for ecological cultivation in this embodiment comprises the following specific steps:
s1, selecting microorganisms: selecting proper microorganism types according to the requirements of ecological cultivation;
in step S1, microorganisms commonly used in ecological cultivation include probiotics including photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes and filamentous bacteria, aerobic microorganisms including nitrifying bacteria, vulcanizing bacteria and iron bacteria, and spoilage microorganisms including anaerobic bacteria and gas producing bacteria, wherein the photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes and filamentous bacteria are composed in a ratio of 1:2:2:1:1.
S2, collecting and culturing microorganisms: culturing microorganisms collected from natural environment;
in step S2, the microorganisms collected from the natural environment may be isolated and purified to obtain single strains, and the single strains may be transferred to a new culture medium using a conventional coating method or a dilution coating method to obtain pure cultures, and then the strains may be cultured to provide a culture medium and conditions for promoting the propagation and growth of the microorganisms; the method comprises the following steps of:
a. sampling: collecting a sample from a natural environment;
b. dilution: subjecting the sample to appropriate dilution to ensure isolation of individual microbial cells;
c. separating: distributing the diluted sample on a nutrient-rich culture medium, and utilizing different culture conditions such as temperature, pH value, nutrient concentration and the like to promote the growth of microbial cells;
d. purifying: by stepwise dilution and re-isolation, individual colonies are selected, each from a single microbial cell, so that a pure single strain can be obtained.
S3, screening and identifying microorganisms: selecting a strain with good characteristics by screening and identifying microorganisms;
in step S3, strains with good characteristics are selected by screening and identifying microorganisms, and can be evaluated by observing the morphology, physiological characteristics and promotion effect on ecological cultures, and the strains are identified by using modern molecular biology techniques and biochemical methods, including morphological identification, physiological and biochemical characteristics identification, molecular biology identification and antibiotic susceptibility testing, morphological identification: observing morphological features of the microorganism, such as colony shape, size and color, and observing cell morphology and structure using a microscope; and (3) identifying physiological and biochemical characteristics: performing a series of physiological biochemical experiments, such as enzyme activity assays and metabolite tests, to determine the metabolic characteristics of the microorganism; molecular biology identification: genetic analysis of microorganisms to determine their species or strain using molecular biological techniques such as PCR and sequence analysis; antibiotic susceptibility testing: the susceptibility of microorganisms to different antibiotics is determined using an antibiotic susceptibility test to direct clinical treatment or other applications.
S4, preparing a microbial preparation: culturing and amplifying the screened strain to prepare a microbial preparation;
in the step S4, the screened strain is cultured and amplified, and is prepared into a microbial preparation, wherein the preparation can be in the form of a liquid culture, a solid culture or powder, and the microbial preparation is prepared by culturing and amplifying, and is operated according to the following steps:
A. culturing the amplified strain: inoculating the screened strain into a proper culture medium, and providing proper temperature, pH and nutrition conditions to promote the growth and propagation of the strain;
B. strain purification: isolation of individual colonies onto new medium until pure strains are obtained;
C. strain storage: for long-term storage and backup of strains, cryopreservation or preparation of lyophilisates is used;
D. preparing a microbial preparation: preparing into microbial preparation, and packaging the microbial preparation into proper package;
wherein, the cryopreservation is to preserve the strain at a low temperature of-80 ℃.
S5, applying a microbial preparation: and applying the prepared microbial preparation to an ecological breeding environment.
In step S5, the prepared microbial preparation may be applied by being added to feed or sprayed in a body of aquaculture water.
According to the preparation method of the microorganism for ecological cultivation, the proper microorganism type is selected according to the requirements of ecological cultivation, the collected microorganism is cultivated, the microorganism is screened and identified, the strain with good characteristics is selected, the screened strain is cultivated and amplified, the microbial preparation is prepared, and the prepared microbial preparation is applied to an ecological cultivation environment, so that the transformation function is realized, the risks of various costs are reduced, and the microbial preparation has an obvious promotion effect on cultivated animals and plants.
The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention are intended to be within the scope of the present invention as claimed.
Claims (9)
1. The microbial preparation method for ecological cultivation is characterized by comprising the following specific steps:
s1, selecting microorganisms: selecting proper microorganism types according to the requirements of ecological cultivation;
s2, collecting and culturing microorganisms: culturing microorganisms collected from natural environment;
s3, screening and identifying microorganisms: selecting a strain with good characteristics by screening and identifying microorganisms;
s4, preparing a microbial preparation: culturing and amplifying the screened strain to prepare a microbial preparation;
s5, applying a microbial preparation: and applying the prepared microbial preparation to an ecological breeding environment.
2. A method for preparing microorganisms for ecological breeding as claimed in claim 1, wherein in step S1, microorganisms commonly used in ecological breeding include probiotics, aerobic microorganisms and bad oxygen microorganisms.
3. A method for preparing microorganisms for ecological breeding according to claim 2, wherein in step S1, the probiotics include photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes and filamentous bacteria, wherein the photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes and filamentous bacteria are composed according to 1:2:2:1:1, and the aerobic microorganisms are composed of nitrifying bacteria, vulcanizing bacteria and iron bacteria, and the bad oxygen microorganisms include anaerobic bacteria and gas generating bacteria.
4. A method for preparing microorganisms for ecological breeding according to claim 3, wherein in step S2, the microorganisms collected from the natural environment can be obtained as single strains by separation and purification, single colonies are transferred to a new culture medium using a conventional coating method or a dilution coating method to obtain pure cultures, and then the strains are cultured to provide culture medium and conditions for promoting the propagation and growth of microorganisms;
the method comprises the following steps of:
a. sampling: collecting a sample from a natural environment;
b. dilution: subjecting the sample to appropriate dilution to ensure isolation of individual microbial cells;
c. separating: distributing the diluted sample on a nutrient-rich culture medium, and utilizing different culture conditions such as temperature, pH value, nutrient concentration and the like to promote the growth of microbial cells;
d. purifying: by stepwise dilution and re-isolation, individual colonies are selected, each from a single microbial cell, so that a pure single strain can be obtained.
5. A method for preparing microorganisms for ecological breeding as claimed in claim 4, wherein in step S3, strains with good characteristics are selected by screening and identifying microorganisms, and can be evaluated by observing the morphology, physiological characteristics and promotion effect on ecological breeding, and the strains are identified by using modern molecular biology techniques and biochemical methods, including morphological identification, physiological and biochemical characteristics identification, molecular biology identification and antibiotic susceptibility test.
6. The method of claim 5, wherein in step S3, morphological identification: observing morphological features of the microorganism, such as colony shape, size and color, and observing cell morphology and structure using a microscope;
and (3) identifying physiological and biochemical characteristics: performing a series of physiological biochemical experiments, such as enzyme activity assays and metabolite tests, to determine the metabolic characteristics of the microorganism;
molecular biology identification: genetic analysis of microorganisms to determine their species or strain using molecular biological techniques such as PCR and sequence analysis;
antibiotic susceptibility testing: the susceptibility of microorganisms to different antibiotics is determined using an antibiotic susceptibility test to direct clinical treatment or other applications.
7. The method of claim 6, wherein in step S4, the selected strain is cultured and amplified to prepare a microbial preparation, which can be in the form of liquid culture, solid culture or powder.
8. The method for preparing microorganisms for ecological breeding as claimed in claim 7, wherein in the step S4, the microorganism preparation is prepared by culture amplification, and is operated according to the following steps:
A. culturing the amplified strain: inoculating the screened strain into a proper culture medium, and providing proper temperature, pH and nutrition conditions to promote the growth and propagation of the strain;
B. strain purification: isolation of individual colonies onto new medium until pure strains are obtained;
C. strain storage: for long-term storage and backup of strains, cryopreservation or preparation of lyophilisates is used;
D. preparing a microbial preparation: preparing into microbial preparation, and packaging the microbial preparation into proper package;
wherein, the cryopreservation is to preserve the strain at a low temperature of-80 ℃.
9. A method of preparing microorganisms for ecological breeding as claimed in claim 8, wherein in step S5, the prepared microorganism preparation is applied by adding to feed or spraying in a body of aquaculture water.
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| CN118086056A (en) * | 2024-04-25 | 2024-05-28 | 内蒙古师范大学 | Development and monitoring method of growth-promoting anti-germ agent |
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