CN117551531A - Medical science inspection microorganism culture apparatus - Google Patents

Medical science inspection microorganism culture apparatus Download PDF

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Publication number
CN117551531A
CN117551531A CN202311501677.9A CN202311501677A CN117551531A CN 117551531 A CN117551531 A CN 117551531A CN 202311501677 A CN202311501677 A CN 202311501677A CN 117551531 A CN117551531 A CN 117551531A
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CN
China
Prior art keywords
incubator
culture dish
microorganism
motor
culture
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CN202311501677.9A
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Chinese (zh)
Inventor
刘倩
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Changzhou Wujin People's Hospital Changzhou Eighth People's Hospital
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Changzhou Wujin People's Hospital Changzhou Eighth People's Hospital
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Priority to CN202311501677.9A priority Critical patent/CN117551531A/en
Publication of CN117551531A publication Critical patent/CN117551531A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14Incubators; Climatic chambers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Abstract

The invention discloses a medical examination microorganism culture device in the technical field of medical examination, which comprises an incubator, wherein constant temperature and constant humidity control equipment is connected inside the incubator, a display is connected to the surface of the incubator, the constant temperature and constant humidity control equipment is electrically connected with the display, an opening is formed in the surface of the incubator, a box door is arranged at the opening, and a microorganism observation mechanism is arranged inside the incubator; this scheme is through the inside sealed setting of incubator, installs multiunit culture dish on incubator internally mounted's the microorganism observation mechanism simultaneously to install inoculation mechanism on the incubator, can carry out microorganism inoculation in the multiunit culture dish through inoculation mechanism, and be in the closed environment of incubator inside throughout the inoculation process, even follow-up microorganism inoculation or culture substance add in time, all be in under sealed aseptic environment, avoid the microorganism in the culture dish to be polluted in the cultivation process or in the inoculation process or in the observation process.

Description

Medical science inspection microorganism culture apparatus
Technical Field
The invention relates to the technical field of medical examination, in particular to a medical examination microorganism culture device.
Background
Microbial culture is one of the important technologies in clinical medicine and life sciences research. By culturing microorganisms, information such as the growth condition of the microorganisms, identification of the types of the microorganisms, sensitivity to antibiotics and the like can be obtained, and important basis is provided for clinical diagnosis and treatment. Conventional microorganism culture requires addition of a culture medium to a culture dish and then culture in an incubator.
Through retrieving, chinese patent number CN201710204226.7 discloses a medical science inspection microorganism culture apparatus, including the incubator casing, upper left side is equipped with the protecting crust in the incubator casing, be equipped with the division board in the protecting crust, the division board is cut apart into four independent regions in with the protecting crust, all be equipped with a cultivation storehouse rack in every region, cultivate all be equipped with on the rack and cultivate the storehouse, cultivate the storehouse and be equipped with and cultivate the storehouse hatch door, cultivate including cultivating the storehouse casing in the storehouse, cultivate and be provided with two cultivation regions in the storehouse, be first cultivation region and second cultivation region respectively, second cultivation region bottom is equipped with the base, the base upside is equipped with heating device, the heating device upside is equipped with the temperature buffer layer.
The beneficial effects of the device are as follows: through setting up the incubator that keeps warm shading and confined, establish a plurality of cultivation storehouses simultaneously in breeding device, therefore can carry out the experiment of cultivating in the same time of many bacterial, can carry out accurate regulation and control to temperature, humidity, illumination intensity and gaseous environment at the in-process of cultivating, it has most suitable cultivation environment to have guaranteed to cultivate the microorganism, simultaneously at the in-process of cultivating can carry out the observation of real-time cultivation state and the observation of later stage through the camera, need not open the cultivation storehouse in the in-process of observing moreover, can not cause the destruction to the environment of cultivating the storehouse, the stability and the persistence of cultivated microorganism have been guaranteed, in addition, setting up parameter and real-time monitoring parameter to the environment of cultivating the storehouse and all can carry out real-time display through the display.
In the use process of the existing microorganism culture device, a plurality of culture dishes can be simultaneously arranged to serve as an experiment group and a control group, and the experiment group and the control group are controlled by automatic equipment to be cultivated in a constant temperature and humidity environment. However, in the process of culturing microorganisms, the prepared culture medium needs to be inoculated, so that the culture medium needs to be exposed to the external environment after being prepared, and the culture medium is further polluted. And the experimental group and the control group are inoculated, and repeated operation is needed for a plurality of times, so that the same inoculation condition of the experimental group and the control group cannot be ensured even in a sterile environment, and further, the difference in microorganism culture is caused. To this end, we propose a medical test microorganism culture apparatus.
Disclosure of Invention
The present invention is directed to a medical test microorganism culture apparatus for solving the problems set forth in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a medical science inspection microorganism culture apparatus, includes the incubator, the internally connected of incubator has constant temperature and humidity controlgear, the surface connection of incubator has the display, constant temperature and humidity controlgear and display electric connection, the surface of incubator is provided with opening and opening part and installs the chamber door, the internally mounted of incubator has microorganism observation mechanism, install a plurality of groups culture dish on the microorganism observation mechanism, the surface mounting of incubator has the microscope, the one end of microscope is inserted and is established in the incubator inside and be located microorganism observation mechanism top, the microscope is used for running through in the culture dish, the internally mounted of incubator has moving mechanism, install fixed establishment on the moving mechanism, fixed establishment is used for the centre gripping culture dish, moving mechanism is used for moving the culture dish, the surface mounting of incubator has inoculation mechanism, inoculation mechanism is used for planting into the culture dish.
Preferably, the microorganism observation mechanism comprises a motor A arranged inside an incubator, a rotary drum is connected to an output shaft of the motor A, a connecting rod is connected to the surface of the rotary drum, a fixed sleeve is connected to the other end of the connecting rod, a culture dish is inserted into the fixed sleeve, the microorganism observation mechanism further comprises a motor B arranged inside the incubator, the motor B is positioned on one side of the motor A, an installation seat is connected to the inner wall of the incubator, a screw rod A is inserted into the installation seat, an output shaft of the motor B is connected with the screw rod A in a transmission manner, a lifting plate is arranged on the surface of the screw rod A in a matched manner, the lifting plate is positioned below the microscope, and a groove is formed in the surface of the fixed sleeve, and the size of the lifting plate is matched with the groove.
Preferably, the surface of lifter plate is connected with the ejector pad, the lower extreme surface of culture dish is provided with the kerve, ejector pad, kerve looks adaptation and all set up to the frustum form.
Preferably, the upper end surface of the culture dish is connected with a sealing cover, the sealing cover is made of transparent materials, an opening is formed in the surface of the sealing cover, and a membrane flap is connected to the opening of the sealing cover.
Preferably, the moving mechanism comprises two groups of vertical limit vertical rods arranged inside the incubator, a motor C is arranged between the two groups of limit vertical rods, a screw rod B is connected to an output shaft of the motor C, the screw rod B and the limit vertical rods are distributed in parallel, a lifting table A matched with the surface of the limit vertical rods and the screw rod B is arranged on the surface of the lifting table A, a U-shaped frame is connected to the surface of the lifting table A, a screw rod C and a limit cross rod are inserted into the U-shaped frame, the screw rod C, the limit cross rod and the screw rod B are vertically distributed, a sliding table matched with the surface of the screw rod C and the surface of the limit cross rod are connected with a motor D, an output shaft of the motor D is connected with the screw rod C in a transmission mode, a sliding carriage is connected to the surface of the sliding table, and a plurality of groups of fixing mechanisms are arranged on the surface of the sliding carriage.
Preferably, the fixed establishment is including connecting the mounting panel on the balladeur train surface, the inside of mounting panel is inserted and is equipped with driving shaft, driven shaft, driving shaft, driven shaft's surface is connected with initiative arm lock, driven arm that adds respectively, the surface of culture dish is provided with the draw-in groove, the size of draw-in groove and initiative arm lock, driven arm that adds looks adaptation make the lower extreme of driving shaft is connected with the driving gear, the lower extreme of driven shaft is connected with driven gear, driving gear and driven gear meshing are connected, the surface connection of mounting panel has motor E, motor E's output shaft and driving shaft transmission are connected.
Preferably, the inoculation mechanism comprises a mounting frame arranged on the surface of the incubator, a sleeve frame which is in equidistant linear distribution is connected to the surface of the mounting frame, each group of sleeve frames are internally and fixedly connected with a liquid injection pipe, the lower end of each liquid injection pipe is connected with a communicating pipe, the lower end of each communicating pipe penetrates through the incubator and is inserted into the incubator, and the size of each communicating pipe is matched with the size of each membrane flap.
Preferably, the surface of annotating the liquid pipe is connected with the branch pipe, the branch pipe is linked together with annotate the liquid intraductal, annotate the junction of liquid pipe and communicating pipe and be provided with the check valve, annotate the inside of liquid pipe and insert and be equipped with the piston, the upper end of piston is connected with the push rod.
Preferably, the surface of the liquid injection pipe is provided with scales.
Preferably, the damping slide bar is inserted on the surface of mounting bracket, the upper end of damping slide bar is connected with and connects soon, connect quick surface and be provided with the through-hole that is equidistant linear distribution, the push rod upper end is all inserted and is established in the through-hole of connecting fast surface, connect quick surface and be provided with screw hole and through-hole and be linked together, connect quick screw hole interpolation and be equipped with stop bolt, the lower extreme of damping slide bar is inserted and is established at the intraductal, the sleeve pipe is connected at the surface of incubator, sheathed tube internal connection has the spring, the upper end of spring is connected on the damping slide bar.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the scheme, after the temperature and humidity of the incubator are regulated through the internal sealing arrangement of the incubator, the temperature and humidity environment in the incubator can be kept, meanwhile, a plurality of groups of culture dishes are arranged on a microorganism observation mechanism arranged in the incubator, the same or different culture mediums can be configured through the plurality of groups of culture dishes, microorganisms are further injected into the culture dishes to be cultured under different conditions or cultured under the same conditions, an experimental group and a control group for culturing the microorganisms can be arranged, an inoculation mechanism is arranged on the incubator, microorganisms can be inoculated in the plurality of groups of culture dishes through the inoculation mechanism, and the inoculation process is always in a closed environment in the incubator, even if subsequent microorganisms are inoculated or culture substances are added, the subsequent microorganisms in the culture dishes are all in a sealed sterile environment, and the microorganisms in the culture dishes are prevented from being polluted in the culture process or the inoculation process or the observation process;
2. according to the technical scheme, the microscope is arranged in the incubator, the culture dish can be driven to carry out position adjustment in the incubator through the microorganism observation mechanism, and further under the condition that the culture dish is not taken out or opened, a user can observe the microorganism culture condition in the culture dish through the microscope, so that the situation that microorganisms need to be frequently taken out from a sealed sterile environment in the observation process is avoided, and the possibility of pollution of a microorganism culture medium is increased;
3. this scheme is through moving mechanism, fixed establishment's structure setting, can carry out the centre gripping to the multiunit culture dish simultaneously and fix and remove, makes inoculation mechanism can carry out the microorganism liquid inoculation of the same or different grade, dose in a plurality of culture dishes simultaneously to improve this device and use the high efficiency.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the incubator of the present invention;
FIG. 3 is a schematic view showing the structural installation of the microorganism observing mechanism of the present invention;
FIG. 4 is a schematic view of a part of the structure of the microorganism observing mechanism of the present invention;
FIG. 5 is a schematic view of the mounting structure of the inoculating mechanism of the present invention;
FIG. 6 is a schematic diagram of a moving mechanism according to the present invention;
FIG. 7 is a schematic view of a fixing mechanism according to the present invention;
FIG. 8 is a schematic view of the inoculating mechanism of the present invention;
FIG. 9 is a schematic view of a portion of the inoculating mechanism of the present invention;
FIG. 10 is a schematic view of a part of the inoculating mechanism of the present invention.
In the figure: 1. an incubator; 2. a microorganism observation mechanism; 201. a motor A; 202. a rotating drum; 203. a connecting rod; 204. a fixed sleeve; 205. slotting; 206. a culture dish; 207. a motor B; 208. a mounting base; 209. a screw rod A; 210. a lifting plate; 211. a pushing block; 212. a bottom groove; 213. sealing cover; 214. a membrane flap; 215. a clamping groove; 3. a microscope; 4. constant temperature and humidity control equipment; 5. a display; 6. a moving mechanism; 601. a limit vertical rod; 602. a motor C; 603. a screw rod B; 604. a lifting platform A; 605. a U-shaped frame; 606. a screw rod C; 607. a limiting cross bar; 608. a sliding table; 609. a motor D; 610. a carriage; 7. a fixing mechanism; 701. a mounting plate; 702. a driving shaft; 703. a driven shaft; 704. an active clamp arm; 705. a driven arm; 706. a drive gear; 707. a driven gear; 708. a motor E; 8. an inoculation mechanism; 801. a mounting frame; 802. a sleeve frame; 803. a liquid injection pipe; 804. a communicating pipe; 805. a branch pipe; 806. a scale; 807. a one-way valve; 808. a piston; 809. a push rod; 810. damping slide bar; 811. the connection is quick; 812. a limit bolt; 813. a sleeve; 814. a spring; 9. a box door.
Detailed Description
Example 1
Referring to fig. 1-10, the present invention provides a technical solution:
the utility model provides a medical science inspection microorganism culture apparatus, the internally mounted of incubator 1 has microorganism observation mechanism 2, installs multiunit culture dish 206 on the microorganism observation mechanism 2, can dispose the same or different culture medium through multiunit culture dish 206, further pours into the microorganism into in the culture dish 206, carries out the cultivation of different conditions or the same condition and cultivates, can realize the experimental group and the control group setting of microorganism cultivation. Meanwhile, the constant temperature and humidity control equipment 4 is installed in the incubator 1, and the constant temperature and humidity control equipment 4 further controls the microorganisms on the plurality of groups of culture dishes 206 to be cultured in the same temperature and humidity environment due to the fact that the temperature and humidity inside the incubator 1 are controlled, and the same environment is further kept, so that the culture distinction of the microorganisms in different inoculation environments is checked. And be connected with display 5 on the constant temperature and humidity control equipment 4, display 5 installs the surface at incubator 1, can carry out real-time supervision to the humiture environment in the incubator 1 through display 5. Meanwhile, the microscope 3 is arranged above the incubator 1, and a plurality of groups of culture dishes 206 arranged on the microorganism observing mechanism 2 can be directly observed through the microscope 3, so that the culture condition of microorganisms can be recorded in real time. And the inside of incubator 1 is located one side of microorganism observation mechanism 2 and installs mobile mechanism 6, installs a plurality of fixed establishment 7 on the mobile mechanism 6, can carry out the centre gripping to the single culture dish 206 on the microorganism observation mechanism 2 through fixed establishment 7 and fix, further drive fixed establishment 7 through mobile mechanism 6 and shift, can make the inoculation mechanism 8 of incubator 1 top installation inoculate the microorganism in the culture dish 206 of centre gripping on the fixed establishment 7. The front end surface of incubator 1 is provided with opening and installs chamber door 9 on the opening, can realize the inside sealed control of incubator 1 through opening and shutting of chamber door 9, further keep the inside disinfection back of incubator 1, microorganism observation mechanism 2 is in under aseptic environment, and the culture dish 206 of installing on the microorganism observation mechanism 2 is in under the confined environment all the time after the culture medium configuration, even when follow-up microorganism inoculation or culture substance add, all is in under sealed aseptic environment, avoids microorganism in the culture dish 206 to be polluted in the cultivation process or inoculation process or observation process.
The microorganism observation mechanism 2 comprises a motor A201 arranged inside the incubator 1, a rotary drum 202 is connected to an output shaft of the motor A201, a connecting rod 203 is connected to the surface of the rotary drum 202, a fixing sleeve 204 is connected to the other end of the connecting rod 203, a culture dish 206 is inserted and arranged in the fixing sleeve 204, and the culture dish 206 is supported by the fixing sleeve 204 on the culture dish 206, so that the culture dish 206 can be fixedly arranged on the microorganism observation mechanism 2. And the motor A201 can be started to rotate the front rotary drum 202, further drives the fixed sleeve 204 to connect with the culture dish 206 for rotation, and controls the culture dish 206 to move in the incubator 1. The upper end surface of the incubator 1 is connected with a microscope 3, one end of the microscope 3 is inserted inside the incubator 1, and the microscope 3 is in the rotation locus of the culture dish 206. Further, in the process of driving the culture dish 206 to rotate by the motor A201, when the culture dish 206 rotates below the microscope 3, a user can observe the culture condition of microorganisms in the culture dish 206 through the space between the microscopes 3 under the condition that the incubator 1 is not opened outside the incubator 1, so that the probability that microorganisms need to be frequently taken out from a sealed sterile environment in the observation process, and the probability of contamination of a microorganism culture medium is increased.
The microorganism observing mechanism 2 further comprises a motor B207 installed inside the incubator 1, the motor B207 being located at one side of the motor A201. The inner wall of the incubator 1 is connected with a mounting seat 208, a screw A209 is inserted in the mounting seat 208, and an output shaft of a motor B207 is in transmission connection with the screw A209. Meanwhile, a lifting plate 210 is installed on the surface of the screw rod A209 in a matched mode, and the lifting plate 210 is located below the microscope 3. The motor B207 is started to drive the screw rod A209 to rotate, the lifting plate 210 is further controlled to linearly slide on the surface of the screw rod A209, the culture dish 206 can be taken down from the fixed sleeve 204 in the sliding process of the lifting plate 210, the culture dish 206 is lifted to the lower side of the microscope 3, and a user can directly observe the microorganism cultivation condition in the culture dish 206 through the microscope 3. The surface of lifter plate 210 is connected with ejector pad 211, and the lower extreme surface of culture dish 206 is provided with kerve 212 simultaneously, and ejector pad 211, kerve 212 looks adaptation and all set up to the frustum form for lifter plate 210 promotes ejector pad 211 and inserts in kerve 212 in the ascending process, further makes culture dish 206 card at the upper end fixed surface of ejector pad 211, makes lifter plate 210 can be with culture dish 206 stable upwards lifting, avoids culture dish 206 to take place to drop at the lifting in-process and leads to microorganism cultivation environment to be destroyed. Meanwhile, the surface of the fixing sleeve 204 is provided with the slot 205, the size of the lifting plate 210 is matched with the slot 205, so that the lifting plate 210 can be inserted into the slot 205 and lift the culture dish 206 through the push block 211 in the lifting process, and the lifting plate 210 can smoothly pass through the fixing sleeve 204, so that the lifting control of the culture dish 206 is realized.
The upper end surface of the culture dish 206 is connected with a sealing cover 213, and the sealing cover 213 is made of transparent materials, so that the culture dish 206 is convenient to be protected from pollution and the microorganism culture condition in the culture dish 206 is convenient to be observed. Simultaneously, the surface of the sealing cover 213 is provided with an opening, the opening is connected with a membrane flap 214, the opening on the surface of the sealing cover 213 can be sealed through the membrane flap 214, and meanwhile, the membrane flap 214 can be quickly penetrated by hardware so as to open the culture dish 206, and further subsequent operations such as microorganism inoculation and the like are conveniently carried out in the culture dish 206.
The upper end surface of the incubator 1 is provided with an inoculation mechanism 8, and the inoculation mechanism 8 can inoculate a culture dish 206 placed inside the incubator 1. The inoculation mechanism 8 comprises a mounting frame 801 arranged on the surface of the incubator 1, the surface of the mounting frame 801 is connected with sleeves 802 which are distributed linearly at equal distance, each group of sleeves 802 is fixedly connected with a liquid injection pipe 803, the lower end of each liquid injection pipe 803 is connected with a communicating pipe 804, and the lower end of each communicating pipe 804 penetrates through the incubator 1 and is inserted into the incubator 1. Further, when the culture medium in the culture dish 206 needs to be inoculated with microorganisms, the microorganisms to be inoculated are further transferred to the incubator 1 through the communicating tube 804 by adding the microorganisms to be inoculated into the liquid injection tube 803. The internally mounted of incubator 1 has mobile mechanism 6, installs fixed establishment 7 on the mobile mechanism 6, can take off culture dish 206 from microorganism observation mechanism 2 through fixed establishment 7, further moves culture dish 206 to inoculation mechanism 8's below through mobile mechanism 6, because the size of communicating pipe 804 and the size looks adaptation of membrane lamella 214, makes communicating pipe 804 insert and establishes in membrane lamella 214 after, can be with the microorganism seal inoculation in the notes liquid pipe 803 to culture dish 206 in to avoid culture dish 206 to be opened at inoculation microorganism in-process, lead to the interior contaminated of culture dish 206.
The moving mechanism 6 comprises two groups of vertical limiting vertical rods 601 arranged inside the incubator 1, a motor C602 is arranged between the two groups of limiting vertical rods 601, a screw rod B603 is connected to an output shaft of the motor C602, and the screw rod B603 and the limiting vertical rods 601 are distributed in parallel. The surface mounting of limit montant 601, lead screw B603 has the elevating platform A604 of looks adaptation, drives lead screw B603 and rotates after opening through motor C602, further controls elevating platform A604 at the linear slip of limit montant 601, the surface of lead screw B603 to realize elevating platform A604's lift. The fixing mechanism 7 is arranged on the lifting platform A604, and after the culture dish 206 is clamped and fixed by the fixing mechanism 7, the lifting of the culture dish 206 in the incubator 1 is controlled. The surface of elevating platform A604 is connected with U type frame 605, and the inside of U type frame 605 is inserted and is equipped with lead screw C606, spacing horizontal pole 607, and lead screw C606, spacing horizontal pole 607 and lead screw B603 are perpendicular distribution to lead screw C606, the surface mounting of spacing horizontal pole 607 have the slip table 608 of looks adaptation. The surface of the U-shaped frame 605 is connected with a motor D609, and an output shaft of the motor D609 is in transmission connection with a screw rod C606. The motor D609 is started to drive the screw rod C606 to rotate, so that the sliding table 608 is further controlled to linearly slide on the surfaces of the screw rod C606 and the limiting cross rod 607, and the sliding table 608 is transversely moved in the incubator 1. The surface of the sliding table 608 is connected with a sliding frame 610, and a plurality of groups of fixing mechanisms 7 are arranged on the surface of the sliding frame 610, so that the relative position adjustment of the plurality of groups of fixing mechanisms 7 and the microorganism observing mechanism 2 is further realized. The culture dishes 206 installed on the microorganism observation mechanism 2 are located in the clamping range of the fixing mechanism 7, so that different fixing mechanisms 7 correspond to different culture dishes 206, multiple groups of culture dishes 206 can be clamped and fixed simultaneously through the fixing mechanism 7, and the culture dishes 206 are moved to the lower part of the inoculation mechanism 8, so that simultaneous inoculation of multiple groups of culture dishes 206 is facilitated.
The fixing mechanism 7 comprises an installation plate 701 connected to the surface of the carriage 610, a driving shaft 702 and a driven shaft 703 are inserted in the installation plate 701, and the surfaces of the driving shaft 702 and the driven shaft 703 are respectively connected with a driving clamping arm 704 and a driven clamping arm 705. The opening and closing actions are realized by controlling the driving clamping arm 704 and the driven adding arm 705 to reversely rotate, and the culture dish 206 can be further clamped and fixed by the driving clamping arm 704 and the driven adding arm 705 so as to realize the position movement of the culture dish 206. Meanwhile, the clamping groove 215 is formed in the surface of the culture dish 206, the size of the clamping groove 215 is matched with that of the driving clamping arm 704 and the driven adding arm 705, and therefore the culture dish 206 can be prevented from falling when the culture dish 206 is clamped and fixed by the fixing mechanism 7. The lower end of the driving shaft 702 is connected with a driving gear 706, the lower end of the driven shaft 703 is connected with a driven gear 707, and the driving gear 706 and the driven gear 707 are in meshed connection. Meanwhile, the surface of the mounting plate 701 is connected with a motor E708, and the output shaft of the motor E708 is in transmission connection with the driving shaft 702. When the fixing mechanism 7 needs to clamp and control the culture dish 206, the motor E708 is started to drive the driving shaft 702 to rotate, the driving shaft 702 simultaneously drives the driving clamping arm 704 and the driving gear 706 to rotate, the driven gear 707 is meshed with the driven gear 707 to rotate in the rotation process of the driving gear 706, the driven gear 707 is connected with the driven shaft 703 to drive the driven clamping arm 705 to rotate, the rotation directions of the driving clamping arm 704 and the driven clamping arm 705 are opposite, and the opening and closing actions between the driving clamping arm 704 and the driven clamping arm 705 are further realized, so that the culture dish 206 is clamped and fixed conveniently.
The surface of the liquid injection pipe 803 is connected with a branch pipe 805, the branch pipe 805 is communicated with the liquid injection pipe 803, and a one-way valve 807 is arranged at the joint of the liquid injection pipe 803 and the communicating pipe 804. After the culture dish 206 is clamped and fixed by the fixing mechanism 7, the culture dish 206 is moved upwards to the lower part of the communicating pipe 804 by the moving mechanism 6, so that the communicating pipe 804 is further inserted into the membrane flap 214, and the communication between the communicating pipe 804 and the culture dish 206 is further realized. At this time, the microorganism to be inoculated is added into the liquid injection pipe 803 through the branch pipe 805, the surface of the liquid injection pipe 803 is provided with scales 806, and the quantity of the microorganism to be inoculated can be penetrated through the scales 806, so that the accurate control of inoculation is facilitated. Meanwhile, a piston 808 is inserted into the liquid injection pipe 803, the upper end of the piston 808 is connected with a push rod 809, the piston 808 is pushed to slide downwards in the liquid injection pipe 803 by the push rod 809, so that the piston 808 is further used for extruding air in the liquid injection pipe 803, microorganism liquid in the liquid injection pipe 803 can be extruded into the culture dish 206 by the one-way valve 807 under the action of air pressure, microorganism inoculation in the culture dish 206 is further realized, and the culture medium in the culture dish 206 can be prevented from being polluted by opening in the inoculation process, so that the convenience of inoculation is improved.
And the liquid filling pipe 803, the communicating pipe 804, the culture dish 206 and the fixing mechanism 7 are all arranged in a plurality of groups. Through the below of a set of culture dish 206 of every fixed establishment 7 centre gripping to communicating pipe 804, the interval looks adaptation of multiunit fixed establishment 7 and multiunit communicating pipe 804, further make multiunit fixed establishment 7 can be linked together multiunit culture dish 206, communicating pipe 804 simultaneously, further make things convenient for follow-up to carrying out synchronous inoculation in the multiunit culture dish 206, improve inoculation convenience. The surface of mounting bracket 801 is inserted and is equipped with damping slide bar 810, the upper end of damping slide bar 810 is connected with and connects fast 811, connect fast 811's surface and be provided with the through-hole that is equidistant linear distribution, every group annotates the inside push rod 809 upper end of inserting of liquid pipe 803 and establish in connecting fast 811's through-hole, connect fast 811's surface simultaneously and be provided with the screw hole and be linked together with the through-hole, connect fast 811's screw hole interpolation and be equipped with limit bolt 812, screw up the conflict at push rod 809's surface through limit bolt 812, can realize that push rod 809 is fixed on connecting fast 811. When the multiple groups of culture dishes 206 are further inoculated synchronously, the damping slide bars 810 can drive the connection blocks 811 to slide downwards, the multiple groups of push rods 809 are further controlled to have equal sliding length in the liquid injection pipe 803, and the microorganism liquid is further added into the multiple groups of culture dishes 206 in an equal amount, so that the accurate control of microorganism inoculation is realized, and the error is reduced. And the multiple groups of liquid injection pipes 803 can be filled with different microorganism liquid, so that different variable control in the culture dish 206 is further realized. And the separation of the push rod 809 and the connecting speed 811 can be realized by unscrewing the limit bolts 812 in the threaded holes, so that the controllable arrangement of the microorganism liquid injection in different liquid injection pipes 803 is further facilitated. The lower end of damping slide bar 810 is inserted in sleeve 813, sleeve 813 is connected on the surface of incubator 1, meanwhile the inside of sleeve 813 is connected with spring 814, and the upper end of spring 814 is connected on damping slide bar 810. When the damping slide bar 810 simultaneously extrudes the spring 814 in the sliding process, after the microorganism liquid in the liquid injection pipe 803 is further added into the specified metering, the damping slide bar 810 rapidly overcomes the negative pressure in the liquid injection pipe 803 to be extracted under the elastic force of the spring 814, so that the error of dosage control is avoided.

Claims (10)

1. The utility model provides a medical science inspection microorganism culture apparatus, includes incubator (1), the internally connected of incubator (1) has constant temperature and humidity controlgear (4), the surface connection of incubator (1) has display (5), constant temperature and humidity controlgear (4) and display (5) electric connection, the surface of incubator (1) is provided with opening and opening part installs chamber door (9), its characterized in that: the utility model provides a culture dish (206) is used for cultivating in culture dish (206), including internally mounted, microorganism observation mechanism (2) of incubator (1), install a plurality of group's culture dish (206) on microorganism observation mechanism (2), surface mounting of incubator (1) has microscope (3), the one end of microscope (3) is inserted and is established inside incubator (1) and be located microorganism observation mechanism (2) top, microscope (3) are used for running through in culture dish (206), internally mounted of incubator (1) has moving mechanism (6), install fixed establishment (7) on moving mechanism (6), fixed establishment (7) are used for centre gripping culture dish (206), moving mechanism (6) are used for removing culture dish (206), surface mounting of incubator (1) has inoculation mechanism (8), inoculation mechanism (8) are used for inoculating in culture dish (206).
2. A medical test microbiological culture device according to claim 1 wherein: the utility model provides a microorganism observation mechanism (2) is including installing motor A (201) inside incubator (1), be connected with rotary drum (202) on the output shaft of motor A (201), the surface connection of rotary drum (202) has connecting rod (203), the other end of connecting rod (203) is connected with fixed cover (204), culture dish (206) are inserted and are established in fixed cover (204), microorganism observation mechanism (2) is still including installing motor B (207) inside incubator (1), motor B (207) are located one side of motor A (201), be connected with mount pad (208) on the inner wall of incubator (1), the inside of mount pad (208) is inserted and is equipped with lead screw A (209), the output shaft and lead screw A (209) transmission of motor B (207) are connected, lifting plate (210) are installed in the surface phase-fit of lead screw A (209), lifting plate (210) are located the below of microscope (3), the surface phase-fit of fixed cover (204) is provided with fluting (205), size and slotting (205) phase-fit.
3. A medical test microbiological culture device according to claim 2 wherein: the surface of lifter plate (210) is connected with ejector pad (211), the lower extreme surface of culture dish (206) is provided with kerve (212), ejector pad (211), kerve (212) looks adaptation and all set up to the frustum form.
4. A medical test microbiological culture device according to claim 2 wherein: the upper end surface of culture dish (206) is connected with sealed lid (213), sealed lid (213) are transparent material, the surface of sealed lid (213) is provided with the opening, the opening part of sealed lid (213) is connected with membrane lamella (214).
5. A medical test microbiological culture device according to claim 1 wherein: the utility model provides a moving mechanism (6) is including installing at inside two sets of perpendicular spacing montants (601) of incubator (1), two sets of install motor C (602) between spacing montant (601), be connected with lead screw B (603) on the output shaft of motor C (602), lead screw B (603) and spacing montant (601) are parallel distribution, the elevating platform A (604) of looks adaptation are installed to the surface mounting of spacing montant (601), lead screw B (603), the surface connection of elevating platform A (604) has U type frame (605), the inside of U type frame (605) has inserted lead screw C (606), spacing horizontal pole (607), lead screw C (606), spacing horizontal pole (607) are perpendicular distribution, the surface mounting of lead screw C (606), spacing horizontal pole (607) has slip table (608) of looks adaptation, the surface connection of U type frame (605) has motor D (609), the output shaft of motor D (609) and lead screw C (606) transmission are connected, the surface connection of slip table (608) has a plurality of carriage (610) fixed surface mounting (610).
6. A medical test microbiological culture device according to claim 5 wherein: the utility model provides a fixed establishment (7) is including connecting mounting panel (701) on balladeur train (610) surface, the inside of mounting panel (701) is inserted and is equipped with driving shaft (702), driven shaft (703), the surface of driving shaft (702), driven shaft (703) is connected with initiative arm lock (704), driven arm (705) respectively, the surface of culture dish (206) is provided with draw-in groove (215), the size of draw-in groove (215) and initiative arm lock (704), driven arm (705) looks adaptation of adding make the lower extreme of driving shaft (702) is connected with driving gear (706), the lower extreme of driven shaft (703) is connected with driven gear (707), driving gear (706) and driven gear (707) meshing are connected, the surface of mounting panel (701) is connected with motor E (708), the output shaft and the driving shaft (702) transmission of motor E (708) are connected.
7. A medical test microbiological culture device according to claim 1 wherein: inoculation mechanism (8) is including installing mounting bracket (801) at incubator (1) surface, the surface connection of mounting bracket (801) has sleeve frame (802) that are equidistant linear distribution, every group equal fixedly connected with notes liquid pipe (803) in sleeve frame (802), the lower extreme of notes liquid pipe (803) is connected with communicating pipe (804), the lower extreme of communicating pipe (804) runs through incubator (1) and inserts the inside of establishing at incubator (1), the size of communicating pipe (804) and the size looks adaptation of membrane lamella (214).
8. The medical test microorganism culture apparatus according to claim 7, wherein: the surface of notes liquid pipe (803) is connected with branch pipe (805), branch pipe (805) are linked together in annotating liquid pipe (803), the junction of annotating liquid pipe (803) and communicating pipe (804) is provided with check valve (807), the inside of annotating liquid pipe (803) is inserted and is equipped with piston (808), the upper end of piston (808) is connected with push rod (809).
9. A medical test microbiological culture device according to claim 8 wherein: the surface of the liquid injection pipe (803) is provided with scales (806).
10. A medical test microbiological culture device according to claim 8 wherein: the damping slide bar (810) is inserted on the surface of mounting bracket (801), the upper end of damping slide bar (810) is connected with and connects soon (811), the surface of connecting soon (811) is provided with the through-hole that is equidistant linear distribution, push rod (809) upper end is all inserted and is established in the through-hole of connecting soon (811) surface, the surface of connecting soon (811) is provided with screw hole and through-hole and is linked together, the screw hole interpolation of connecting soon (811) is equipped with limit bolt (812), the lower extreme of damping slide bar (810) is inserted and is established in sleeve pipe (813), sleeve pipe (813) are connected at the surface of incubator (1), the internal connection of sleeve pipe (813) has spring (814), the upper end of spring (814) is connected on damping slide bar (810).
CN202311501677.9A 2023-11-10 2023-11-10 Medical science inspection microorganism culture apparatus Pending CN117551531A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311501677.9A CN117551531A (en) 2023-11-10 2023-11-10 Medical science inspection microorganism culture apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311501677.9A CN117551531A (en) 2023-11-10 2023-11-10 Medical science inspection microorganism culture apparatus

Publications (1)

Publication Number Publication Date
CN117551531A true CN117551531A (en) 2024-02-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202311501677.9A Pending CN117551531A (en) 2023-11-10 2023-11-10 Medical science inspection microorganism culture apparatus

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Country Link
CN (1) CN117551531A (en)

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