CN117547648A

CN117547648A - Preparation method of meniscus hydrogel material, meniscus manufacturing method and meniscus

Info

Publication number: CN117547648A
Application number: CN202410044800.7A
Authority: CN
Inventors: 余家阔; 许冰冰; 王星; 窦雪宇; 叶景; 宋士堂; 刘威
Original assignee: Institute of Chemistry CAS; Peking University Third Hospital Peking University Third Clinical Medical College
Current assignee: Institute of Chemistry CAS; Peking University Third Hospital Peking University Third Clinical Medical College
Priority date: 2024-01-12
Filing date: 2024-01-12
Publication date: 2024-02-13
Anticipated expiration: 2044-01-12
Also published as: CN117547648B

Abstract

Embodiments of the present disclosure disclose a meniscus hydrogel material preparation method, a meniscus manufacturing method, and a meniscus. One embodiment of the method comprises the following steps: mixing an acryl glycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator and water to obtain a mixed solution; heating the mixed solution to obtain a meniscus hydrogel composite solution; and curing the meniscus hydrogel composite solution to obtain the meniscus hydrogel material. The implementation mode can improve the mechanical property of the hydrogel material, and further improve the repair capability of the manufactured meniscus.

Description

Preparation method of meniscus hydrogel material, meniscus manufacturing method and meniscus

Technical Field

Embodiments of the present disclosure relate to the technical field of biomedical materials, and in particular, to a meniscus hydrogel material preparation method, a meniscus manufacturing method, and a meniscus.

Background

Meniscus is the most damaged structure in human knee joint, and the lack of meniscus can cause abnormal mechanical force environment in the joint, and cause advanced osteoarthritis, and along with the rapid development of tissue engineering technology, hydrogel materials become the preferred materials for promoting the repair of meniscus damage. Currently, hydrogel materials commonly used for making menisci are: the gelatin is used as a raw material to prepare a hydrogel material, and then the hydrogel material is printed in a 3D printing mode to obtain the meniscus.

However, in practice, it has been found that when the above hydrogel materials are used to make menisci, there are often the following technical problems:

first, gelatin is used as a raw material to prepare a hydrogel material, and gelatin is used as a protein, has the property of high-temperature dissolution and low-temperature gelling, and the mechanical property (such as swelling resistance, toughness and the like) of the formed hydrogel is weak, so that the mechanical property of the prepared hydrogel material is weak, and the repair capability of the prepared meniscus is poor.

Second, when the printability of the hydrogel material is low, the accuracy of the shape and size of the tissue engineering meniscus obtained by printing is low, thereby resulting in low applicability of the tissue engineering meniscus.

The above information disclosed in this background section is only for enhancement of understanding of the background of the inventive concept and, therefore, may contain information that does not form the prior art that is already known to those of ordinary skill in the art in this country.

Disclosure of Invention

The disclosure is in part intended to introduce concepts in a simplified form that are further described below in the detailed description. The disclosure is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter.

Some embodiments of the present disclosure propose a meniscus hydrogel material preparation method, a tissue engineering meniscus manufacturing method, and a tissue engineering meniscus to address one or more of the technical problems mentioned in the background section above.

In a first aspect, some embodiments of the present disclosure provide a method of preparing a meniscus hydrogel material, the method comprising: mixing an acryl glycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator and water to obtain a mixed solution; heating the mixed solution to obtain a meniscus hydrogel composite solution; and curing the meniscus hydrogel composite solution to obtain a meniscus hydrogel material.

Optionally, mixing the acryloylglycine monomer, chitosan, methylene bisacrylamide aqueous solution, photoinitiator and water to obtain a mixed solution, wherein the mixing step comprises the following steps: mixing an acryloylglycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator and water by using an oscillator to obtain a mixed solution, wherein the content of the acryloylglycine monomer ranges from 0.1 to 10 g, the mass fraction of the chitosan ranges from 0.2% to 10%, the concentration of the methylene bisacrylamide aqueous solution ranges from 5 mg/ml to 20 mg/ml, and the mass fraction of the photoinitiator ranges from 0.1% to 1%.

In a second aspect, some embodiments of the present disclosure provide a tissue engineering meniscus manufacturing method comprising: acquiring meniscus image data; generating meniscus point cloud data according to the meniscus image data; generating a meniscus curved surface model according to the meniscus point cloud data; generating a meniscus mould three-dimensional model according to the meniscus curved surface model; controlling an associated mold manufacturing device to manufacture a meniscus transparent mold according to the meniscus mold three-dimensional model; controlling an associated meniscus injection device to inject a prefabricated meniscus hydrogel material into the meniscus transparent mould to obtain a meniscus for tissue engineering to be solidified, wherein the prefabricated meniscus hydrogel material is prepared by the meniscus hydrogel material preparation method described in any implementation manner of the first aspect; and controlling the associated irradiation equipment to irradiate the tissue engineering meniscus to be coagulated to obtain the tissue engineering meniscus.

Optionally, generating a three-dimensional model of the meniscus mould according to the meniscus curved surface model comprises: shearing the meniscus curved surface model to obtain a meniscus model with a vertical section, an upper intersecting line and a lower intersecting line; generating a meniscus column model according to the meniscus curved surface model and the meniscus model with the vertical section; parting the meniscus column model according to the upper intersecting line and the lower intersecting line to obtain a meniscus upper die model and a meniscus lower die model; the above-mentioned meniscus mould model and the above-mentioned meniscus lower mould model are determined as a meniscus mould three-dimensional model.

Optionally, the above-mentioned mold manufacturing apparatus includes a machining device, a spark discharge erosion device, and a meniscus mold injection device; and the control-related mold manufacturing apparatus manufacturing a meniscus transparent mold based on the meniscus mold three-dimensional model, including: controlling the machining equipment to process according to the meniscus mould three-dimensional model to obtain a meniscus mould male mould; controlling the spark discharge corrosion equipment to perform spark discharge corrosion treatment on the meniscus mould male die to obtain a meniscus mould cavity; and controlling the meniscus mould injection equipment to inject the prefabricated transparent injection molding material into the meniscus mould cavity to obtain the meniscus transparent mould.

Optionally, the meniscus transparent mold includes an upper mold and a lower mold, where the upper mold and the lower mold cooperate.

Optionally, a gate is provided on the upper surface of the upper mold.

Optionally, a riser is further disposed on the upper surface of the upper mold.

Optionally, a positioning groove is formed in the upper die, a positioning column is arranged on the upper surface of the lower die, and the positioning groove is in interference fit with the positioning column.

In a third aspect, some embodiments of the present disclosure provide a tissue engineering meniscus prepared by a tissue engineering meniscus manufacturing method as described in any one of the implementations of the second aspect.

The above embodiments of the present disclosure have the following advantageous effects: by the preparation method of the meniscus hydrogel material, the mechanical property of the hydrogel material can be improved, and the repair capability of the manufactured meniscus is further improved. Specifically, the mechanical properties of the fabricated hydrogel material are weak, and the repair capability of the fabricated meniscus is poor because: gelatin is used as a raw material to prepare a hydrogel material, and the gelatin is used as a protein, has the property of high-temperature dissolution and low-temperature gelling, and the mechanical property (such as swelling resistance, toughness and the like) of the hydrogel formed by the gelatin is weak, so that the mechanical property of the prepared hydrogel material is weak, and the repair capability of the prepared meniscus is poor. Based on the above, according to some embodiments of the present disclosure, a meniscus hydrogel material preparation method includes mixing an acryl glycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator, and water to obtain a mixed solution; heating the mixed solution to obtain a meniscus hydrogel composite solution; and curing the meniscus hydrogel composite solution to obtain a meniscus hydrogel material. Because the low-valence metal ions can be used for invasively breaking hydrogen bonds among poly-N-acryloylglycine (PACG) chains and further forming ion complexation with carboxyl groups in PACG polymer chains, and the carboxyl groups can be further complexed with trivalent metal ions in a meniscus repair microenvironment to form a hydrophobic compact layer near the surface of the gel, the mechanical property of the meniscus hydrogel material can be improved. And because oxygen (O2 p) on PACG carboxylate radical in the meniscus hydrogel material can overlap with a 3d orbit in ferric iron in a meniscus repair microenvironment to form a chelating structure, the meniscus hydrogel material can activate hypoxia inducible factor-1α (hypoxia inducible factor-1α, HIF-1α) and inhibit inflammatory reaction, so that the mechanical property and biological function of the hydrogel material can be improved, and the repair capability of the manufactured meniscus can be improved.

Drawings

The above and other features, advantages, and aspects of embodiments of the present disclosure will become more apparent by reference to the following detailed description when taken in conjunction with the accompanying drawings. The same or similar reference numbers will be used throughout the drawings to refer to the same or like elements. It should be understood that the figures are schematic and that elements and components are not necessarily drawn to scale.

FIG. 1 is a flow chart of some embodiments of a method of preparing a meniscus hydrogel material in accordance with the present disclosure;

FIG. 2 is a flow chart of some embodiments of a tissue engineering meniscus manufacturing method according to the present disclosure;

FIG. 3 is a schematic structural view of a meniscal model with vertical section of some embodiments of the tissue engineering meniscal fabrication methods of the present disclosure;

FIG. 4 is a schematic structural view of a three-dimensional model of a meniscus mold of some embodiments of the tissue engineering meniscus manufacturing methods of the present disclosure;

fig. 5 is a schematic structural view of a meniscus transparent mold in some embodiments of tissue engineering meniscus manufacturing methods according to the present disclosure.

Detailed Description

Embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While certain embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete. It should be understood that the drawings and embodiments of the present disclosure are for illustration purposes only and are not intended to limit the scope of the present disclosure.

It should be noted that, for convenience of description, only the portions related to the present invention are shown in the drawings. Embodiments of the present disclosure and features of embodiments may be combined with each other without conflict.

It should be noted that the terms "first," "second," and the like in this disclosure are merely used to distinguish between different devices, modules, or units and are not used to define an order or interdependence of functions performed by the devices, modules, or units.

It should be noted that references to "one", "a plurality" and "a plurality" in this disclosure are intended to be illustrative rather than limiting, and those of ordinary skill in the art will appreciate that "one or more" is intended to be understood as "one or more" unless the context clearly indicates otherwise.

The names of messages or information interacted between the various devices in the embodiments of the present disclosure are for illustrative purposes only and are not intended to limit the scope of such messages or information.

The present disclosure will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

Fig. 1 illustrates a flow 100 of some embodiments of a method of preparing a meniscus hydrogel material in accordance with the present disclosure. The preparation method of the meniscus hydrogel material comprises the following steps:

Step 101, mixing an acryl glycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator and water to obtain a mixed solution.

In some embodiments, the execution body (e.g., industrial robot) of the meniscus hydrogel material preparation method may mix acryl glycine (ACG) monomer, chitosan (Chitosan, CS), methylene bisacrylamide aqueous solution (MBA), photoinitiator (Irgacure) with water by a stirring rod to obtain a mixed solution. Wherein, the content range of the acryloylglycine monomer can be 0.1-10 g. The mass fraction of the chitosan can be in the range of 0.2% -10%. The concentration range of the methylene bisacrylamide aqueous solution can be 5-20 mg/ml. The photoinitiator may be a photoinitiator 2959, or may be lithium-2, 4, 6-trimethylbenzoyl phosphinate.

In some optional implementations of some embodiments, the executing body may further mix the acryloylglycine monomer, chitosan, methylene bisacrylamide aqueous solution, photoinitiator with water by using a shaker to obtain a mixed solution. Wherein, the oscillator can be a circumference oscillator.

And 102, heating the mixed solution to obtain the meniscus hydrogel composite solution.

In some embodiments, the performing body may heat-treat the mixed solution by a water bath heating method to obtain a meniscus hydrogel composite solution. As an example, the above mixed solution may be placed in a water bath at 60 degrees celsius and heated for 5 minutes to obtain a clear and transparent meniscus hydrogel composite solution.

And 103, curing the meniscus hydrogel composite solution to obtain a meniscus hydrogel material.

In some embodiments, the executing body performs curing treatment on the meniscus hydrogel composite solution to obtain a meniscus hydrogel material. In practice, the executing body can irradiate ultraviolet light on the meniscus hydrogel composite solution for 2 hours through an ultraviolet curing lamp to obtain a meniscus hydrogel material, namely semi-interpenetrating network hydrogel PACG-CS-x. Wherein x may represent the mass fraction of chitosan.

The above embodiments of the present disclosure have the following advantageous effects: by the preparation method of the meniscus hydrogel material, the mechanical property of the hydrogel material can be improved, and the repair capability of the manufactured meniscus is further improved. Specifically, the mechanical properties of the fabricated hydrogel material are weak, and the repair capability of the fabricated meniscus is poor because: gelatin is used as a raw material to prepare a hydrogel material, and the gelatin is used as a protein, has the property of high-temperature dissolution and low-temperature gelling, and the mechanical property (such as swelling resistance, toughness and the like) of the hydrogel formed by the gelatin is weak, so that the mechanical property of the prepared hydrogel material is weak, and the repair capability of the prepared meniscus is poor. Based on this, the preparation method of the meniscus hydrogel material of some embodiments of the present disclosure includes firstly, mixing an acryl glycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator and water to obtain a mixed solution; heating the mixed solution to obtain a meniscus hydrogel composite solution; and curing the meniscus hydrogel composite solution to obtain a meniscus hydrogel material. Because the low-valence metal ions can be used for invasively breaking hydrogen bonds among poly-N-acryloylglycine (PACG) chains and further forming ion complexation with carboxyl groups in PACG polymer chains, and the carboxyl groups can be further complexed with trivalent metal ions in a meniscus repair microenvironment to form a hydrophobic compact layer near the surface of the gel, the mechanical property of the meniscus hydrogel material can be improved. And because oxygen (O2 p) on PACG carboxylate radical in the meniscus hydrogel material can overlap with a 3d orbit in ferric iron in a meniscus repair microenvironment to form a chelating structure, the meniscus hydrogel material can activate hypoxia inducible factor-1α (hypoxia inducible factor-1α, HIF-1α) and inhibit inflammatory reaction, so that the mechanical property and biological function of the hydrogel material can be improved, and the repair capability of the manufactured meniscus can be improved.

With further reference to fig. 2, a flow 200 of some embodiments of a tissue engineering meniscus manufacturing method is shown. The flow 200 of the tissue engineering meniscus manufacturing method includes the steps of:

in step 201, meniscus image data is acquired.

In some embodiments, an executing subject (e.g., computing device) of a tissue engineering meniscus manufacturing method may acquire meniscus image data. The meniscus image data may be an image of a meniscus of the target user obtained by scanning with a nuclear magnetic resonance apparatus. The target user may be a user in need of repair or treatment of a meniscus. In practice, the subject may acquire meniscus image data from a database or associated nmr by means of a wired or wireless connection. It should be noted that the wireless connection may include, but is not limited to, a 3G/4G connection, a WiFi connection, a bluetooth connection, a WiMAX connection, a Zigbee connection, a UWB (ultra wideband) connection, and other wireless connection methods now known or developed in the future.

Step 202, generating meniscus point cloud data according to meniscus image data.

In some embodiments, the executing entity may generate meniscus point cloud data from the meniscus image data. The meniscus point cloud data may be point cloud data representing a meniscus. In practice, the execution subject may input the meniscus image data into preset medical reconstruction software to obtain meniscus point cloud data. The file format of the meniscus point cloud data is stl. The preset medical reconstruction software may be preset software capable of converting the imaging data into a three-dimensional model. For example, the preset medical reconstruction software may be a chemicals software or a 3D slider software.

As an example, first, the execution subject may input meniscus image data into the chemicals software. Then, the above-mentioned chemicals software is controlled to execute the editing mask function, the region growing function and the three-dimensional reconstructing function, respectively. And finally, controlling the mic software to output meniscus point cloud data in the stl file format.

Step 203, generating a meniscus curved surface model according to meniscus point cloud data.

In some embodiments, the executing entity may generate a meniscus curved surface model according to the meniscus point cloud data. The meniscus curved surface model may be a model for displaying a meniscus in a curved surface form. In practice, the execution body may input the meniscus point cloud data into preset reverse engineering software to obtain a meniscus curved surface model. The file export format of the meniscus curved surface model may be stp format. The preset reverse engineering software may be preset software for converting 3D scan data or imported file format contents such as STL, OBJ, SAT, PRC, VDA into a three-dimensional model. For example, the preset reverse engineering software may be geomagic software.

As an example, the execution subject may input meniscus point cloud data in stl file format into geomic software, and through curvature detection, a surface patch structure, and grid fitting, a triangle patch surface is fitted to a curved surface, to obtain a meniscus curved surface model. And finally, outputting a file of the meniscus curved surface model in the stp format.

Step 204, generating a meniscus mould three-dimensional model according to the meniscus curved surface model.

In some embodiments, the executing body may generate a three-dimensional model of the meniscus mold from the meniscus curved surface model. The three-dimensional model of the meniscus mold may be a three-dimensional model of a mold for manufacturing a meniscus. In practice, the execution body may input the meniscus curved surface model into preset three-dimensional modeling software to obtain a meniscus mold three-dimensional model. The preset three-dimensional modeling software may be preset software for making a three-dimensional model. For example, the preset three-dimensional modeling software may be UG (Unigraphics NX) software.

As an example, first, the above-described execution subject may input a file of the meniscus surface model in stp format into UG software. And then, performing the mold-turning operation in the UG software to obtain a mold-turning three-dimensional model serving as a meniscus mold three-dimensional model.

In some optional implementations of some embodiments, the executing entity may generate the three-dimensional model of the meniscus mold from the meniscus curved surface model by:

and firstly, shearing the meniscus curved surface model to obtain a meniscus model with a vertical section, an upper intersecting line and a lower intersecting line. In practice, first, the execution subject may input the meniscus curved surface model into the preset three-dimensional modeling software. And secondly, in the preset three-dimensional modeling software, cutting a vertical section along the edge of the meniscus curved surface model to obtain a meniscus model with the vertical section, an upper intersecting line and a lower intersecting line. The upper intersecting line may be an intersecting line obtained by intersecting a vertical tangential plane with the upper surface of the meniscus curved surface model. The lower intersecting line may be an intersecting line obtained by intersecting a vertical tangential plane with the lower surface of the meniscus curved surface model.

By way of example, fig. 3 is a schematic structural view of a vertically sectioned meniscus model of some embodiments of the tissue engineering meniscus manufacturing methods of the present disclosure. Wherein the vertically tangent meniscus model 300 comprises a vertical tangent 301, an upper intersecting line 302 and a lower intersecting line 303.

And secondly, generating a meniscus column model according to the meniscus curved surface model and the meniscus model with the vertical section. In practice, in the preset three-dimensional modeling software, first, the execution body may create a meniscus column model by using the center of gravity of the meniscus curved surface model as the origin. And then, performing Boolean subtraction operation on the newly-built meniscus column body and the meniscus model with the vertical section to obtain a meniscus column body model with a meniscus forming cavity as a meniscus column body model.

And thirdly, performing parting treatment on the meniscus columnar body model according to the upper intersecting line and the lower intersecting line to obtain a meniscus upper die model and a meniscus lower die model. In practice, in the preset three-dimensional modeling software, first, the execution subject may execute the following sub-steps for each intersection included in the intersection line:

And a first sub-step of determining the intersection point as an upper intersection point.

And a second substep of determining a lower intersection point corresponding to the upper intersection point in the lower intersection line as a target intersection point. The lower intersection point may be an intersection point in a lower intersection line. The upper intersection point may be the same as the lower intersection point in the vertical direction.

And a third sub-step of determining a midpoint between the upper intersection point and the target intersection point as a parting point.

And sequentially connecting the determined parting points to obtain a model parting line. And then, the model parting line is projected to the outer surface of the meniscus column model, so as to obtain the model parting surface. Finally, the model located on the upper side of the parting surface of the mold among the above-mentioned meniscus column models is determined as an upper meniscus mold model, and the model located on the lower side of the parting surface of the mold among the above-mentioned meniscus column models is determined as a lower meniscus mold model.

And fourthly, determining the meniscus upper die model and the meniscus lower die model as a meniscus die three-dimensional model.

By way of example, fig. 4 is a schematic structural view of a three-dimensional model of a meniscus mold of some embodiments of the tissue engineering meniscus manufacturing methods of the present disclosure. The three-dimensional model 400 of the meniscus mold includes, among other things, an upper meniscus mold model 401, a lower meniscus mold model 402, and a mold model parting plane 403.

In step 205, an associated mold fabrication apparatus is controlled to fabricate a meniscus transparent mold from a meniscus mold three-dimensional model.

In some embodiments, the execution body may control an associated mold-making device to make a meniscus transparent mold from the meniscus mold three-dimensional model. The transparent mold for meniscus may be a mold made of transparent material for manufacturing meniscus. In practice, the above-described executing bodies may control the associated mold manufacturing apparatus in various ways to manufacture a meniscus transparent mold from the above-described meniscus mold three-dimensional model.

Alternatively, the above-described mold manufacturing apparatus may include a machining device, a spark discharge erosion device, and a meniscus mold injection device. The spark discharge etching apparatus may be an apparatus for etching metal by spark discharge. For example, the spark discharge erosion apparatus may be an electric discharge machine. The above-described meniscus mold injection apparatus may be an injection molding machine for injection molding a meniscus mold.

In some alternative implementations of some embodiments, the executing body may control an associated mold manufacturing apparatus to manufacture the meniscus transparent mold from the meniscus mold three-dimensional model by:

And firstly, controlling the machining equipment to process according to the three-dimensional model of the meniscus mould to obtain a meniscus mould male mould. The male mold of the meniscus mold can be a male mold of the meniscus mold. In practice, the execution body may control the machining apparatus to machine the first meniscus mold with the meniscus mold three-dimensional model as a meniscus mold male mold in accordance with the meniscus mold three-dimensional model.

And secondly, controlling the electric spark discharge corrosion equipment to perform electric spark discharge corrosion treatment on the meniscus mould male die to obtain a meniscus mould cavity. Wherein the meniscus mould cavity may be a cavity for manufacturing a meniscus mould. In practice, the execution body may control the spark discharge erosion device to perform spark discharge erosion treatment on the meniscus mold male mold according to the meniscus mold three-dimensional model, so as to obtain a meniscus mold cavity.

And thirdly, controlling the meniscus mould injection equipment to inject the prefabricated transparent injection molding material into the meniscus mould cavity to obtain the meniscus transparent mould. The prefabricated transparent injection molding material can be a material which is prepared in advance and has material transparency and is used for injection molding. For example, the preformed transparent injection molding material may be melted polycarbonate pellets.

Alternatively, the meniscus transparent mold may include an upper mold and a lower mold. The upper die and the lower die may be matched.

Alternatively, the upper surface of the upper mold may be provided with a gate. In practice, a gate may be provided at the highest point of one side of the upper surface of the upper mold.

Alternatively, the upper surface of the upper mold may be provided with a riser. In practice, the upper die may be provided with a riser at the highest point on the other side of the upper surface.

Optionally, a positioning groove may be disposed in the upper mold. The upper surface of the lower die may be provided with a positioning column. The positioning groove and the positioning column can be in interference fit. The positioning groove may be a notch for positioning the assembly positions of the upper mold and the lower mold.

By way of example, fig. 5 is a schematic structural view of a meniscus transparent mold in some embodiments of tissue engineering meniscus manufacturing methods according to the present disclosure. The meniscus transparent mold 500 includes an upper mold 501, a lower mold 502, a gate 503, a riser 504, and a positioning post 505, among other things.

At step 206, control of an associated meniscus injection device injects a preformed meniscus hydrogel material into the meniscus transparent mold to obtain the tissue engineering meniscus to be solidified.

In some embodiments, the executive body may control an associated meniscus injection device to inject a preformed meniscus hydrogel material into the meniscus transparent mold to obtain the tissue engineering meniscus to be solidified. Wherein the preformed meniscus hydrogel material described above may be obtained by steps 101-103 in those embodiments corresponding to FIG. 1. In practice, the above-described executing bodies may control the associated meniscus injection device in various ways to inject the preformed meniscus hydrogel material into the above-described meniscus transparent mold, resulting in a tissue engineering meniscus to be solidified.

Continuing, in the process of solving the technical problems mentioned in the background art by adopting the technical scheme, the following technical problems are further existed: when the meniscus is manufactured by adopting a fixed die for injection molding, the fluidity of the hydrogel material is poor, and the phenomenon of underinjection can be generated in the manufacturing process, so that the manufactured meniscus has poor quality. Thus, considering that the user's requirements for meniscus quality are high during meniscus use, the following solution is decided to be adopted:

optionally, the meniscus injection device may further include an injection molding assembly, an image acquisition assembly, and a mold adjustment assembly. Wherein, the injection molding component can be a component for injection molding hydrogel materials. The injection molding assembly may include, but is not limited to, an injection molding machine. The image acquisition component may be a component that acquires an image of a meniscus transparent mold. The image acquisition assembly described above may include, but is not limited to, a camera. The above-described mold adjustment assembly may be an assembly for adjusting the angle of a meniscus transparent mold. The above-described die adjustment assembly may include, but is not limited to, an adjustable bracket and a die fixing assembly. The adjustable bracket can be an electric bracket which can not only adjust the angle left and right, but also adjust the angle up and down. The above-described mold-fixing assembly may be an assembly for fixing a meniscus transparent mold. For example, the mold-fixing component may be a clamp for fixing a meniscus transparent mold. The die fixing assembly and the adjustable bracket can be fixedly connected. The meniscus transparent mold may be removably disposed on a mold-holding assembly included in the mold-adjusting assembly. The image acquisition component and the riser can be on the same side so that photographed images can be displayed on the meniscus transparent mold on one side of the riser more clearly.

As an example, in fixing the meniscus transparent mold by the mold fixing assembly, the gates and risers included in the meniscus transparent mold may be respectively provided at the left and right sides of the jig.

In some alternative implementations of some embodiments, the executive body may control the associated meniscus injection device to inject the preformed meniscus hydrogel material into the meniscus transparent mold to obtain the tissue engineering meniscus to be solidified by:

in a first step, the injection molding assembly is controlled to inject a preformed meniscus hydrogel material into the meniscus transparent mold in response to receiving injection molding initiation information. The injection start information may be information indicating start of injection. For example, the injection start information may be "start".

And secondly, controlling the image acquisition component to acquire a first meniscus transparent mold image in response to determining that the current time meets the preset first interval duration condition. The preset first interval duration condition may be that an interval duration between the current time and the time when the injection start information is received is an integer multiple of the preset first interval duration. The preset first interval duration may be a preset interval duration. For example, the preset interval duration may be 2 seconds. The first meniscus transparent mold image described above may be an image of a meniscus transparent mold being injection molded.

And thirdly, performing defect detection processing on the first meniscus transparent mold image to obtain defect detection information. Wherein the defect detection information may characterize whether the first meniscus transparent mold image is defective. The defect detection information may include, but is not limited to, defect type and defect detection area information. The defect type may be indicative of whether the first meniscus transparent mold image is defective. The defect type may be, but is not limited to, one of the following: defective and non-defective. The defect detection area information may characterize the area of the detected defect. It should be noted that, when the defect type indicates that the first meniscus transparent mold image has no defect, the defect detection area information may be empty. The defect detection area information may include defect center point coordinates, defect length, and defect height. The above-described defect center point coordinates may be coordinates of a center point of the defect detection area in the moon plate transparent mold image. The defect detection area may be an area surrounded by a rectangular frame. The defect length may be the length of the defect detection area. The defect height may be a height of the defect detection area. In practice, the execution subject may input the first meniscus transparent mold image into a pre-trained defect detection information generation model to obtain defect detection information. The defect detection information generation model may be a neural network that takes a meniscus transparent mold image as an input and defect detection information as an output. The neural network may be a convolutional neural network.

And a fourth step of determining defect area information according to the defect detection information in response to determining that the defect detection information satisfies a preset detection condition. The preset detection condition may be defect detection information indicating that the first meniscus transparent mold image has a defect. In practice, the execution body may determine, as the defect area information, a product of a defect length and a defect height included in the defect detection information in response to determining that the defect detection information satisfies a preset detection condition.

And fifthly, generating adjustment angle information according to the defect detection information. The angle adjustment information may be information of an angle adjusted by the adjustable bracket. The adjustment angle information may include, but is not limited to, up-down adjustment information and left-right adjustment information. The up-down adjustment information may be information that needs to be adjusted in the up-down direction. The up-down adjustment information may include, but is not limited to, up-down adjustment direction and up-down adjustment angle. The up-down adjustment direction may be a direction in which the adjustable bracket needs to be adjusted in the up-down direction. The up-down adjustment angle may be an angle at which the adjustable bracket is adjusted in the up-down adjustment direction. For example, the above-described up-down adjustment information may be "down adjustment; 15 degrees). The left-right adjustment information may be information that needs to be adjusted in the left-right direction. The left-right adjustment information may include, but is not limited to, a left-right adjustment direction and a left-right adjustment angle. The left-right adjustment direction may be a direction in which the adjustable bracket needs to be adjusted in the left-right direction. The left-right adjustment angle may be an angle at which the adjustable bracket is adjusted in the left-right adjustment direction. For example, the left-right adjustment information may be "left adjustment; 10 degrees). In practice, the execution body may input the defect center point coordinate included in the defect detection information into a preset adjustment angle information generation model to obtain adjustment angle information. The preset adjustment angle information generation model may be a linear function with the coordinates of the defect center point as input and the adjustment angle information as output.

And step six, inputting the defect area information into a preset adjustment information generation model to obtain the adjustment information corresponding to the die adjustment assembly. The preset adjustment information generation model may be a linear function with defect area information as input and adjustment information as output. The adjustment information may be information for the mold adjustment assembly to adjust the meniscus transparent mold. The adjustment information may include, but is not limited to, adjustment speed and adjustment duration. The above-mentioned adjustment speed can be the speed when the adjustable support that the mould adjusting part included rotates when the mould adjusting part adjusts the transparent mould of meniscus. The adjustment time length can be the time length for the adjustable bracket to stay at the position corresponding to the adjustment angle information. It should be noted that the larger the defect area represented by the defect area information, the larger the adjustment speed and adjustment duration.

And seventh, controlling the die adjusting assembly to adjust the meniscus transparent die according to the adjusting speed, the adjusting angle information and the adjusting time length. In practice, first, the execution body may control the adjustable support included in the mold adjustment assembly to adjust the meniscus transparent mold to a position corresponding to the adjustment angle information according to the adjustment speed and the adjustment angle information so that the under-filled region is at the lowest point of the meniscus transparent mold. The adjustable support is then controlled to maintain the meniscus transparent mold for a preset adjustment period. Finally, the adjustable support is controlled to adjust the meniscus transparent mould to the initial position according to the adjusting speed. The initial position may be a position of the meniscus transparent mold at the start of injection molding.

The technical scheme and the related content are taken as an invention point of the embodiment of the disclosure, and the technical problem that when the meniscus is manufactured by adopting a fixed die for injection molding, the fluidity of the hydrogel material is poor, and the phenomenon of underinjection can be generated in the manufacturing process, so that the manufactured meniscus has poor quality is solved. ". Factors that lead to poor quality of the manufactured meniscus tend to be as follows: when the meniscus is manufactured by adopting a fixed die for injection molding, the fluidity of the hydrogel material is poor, and the phenomenon of underinjection can be generated in the manufacturing process, so that the manufactured meniscus has poor quality. If the above factors are solved, the effect of improving the quality of the meniscus can be achieved. To achieve this effect, the tissue engineering meniscus manufacturing method of the present disclosure first controls the injection molding assembly to inject the preformed meniscus hydrogel material into the meniscus transparent mold in response to receiving the injection molding start information. Thus, injection of the preformed meniscus hydrogel material into the meniscus transparent mold may begin. And secondly, controlling the image acquisition component to acquire a first meniscus transparent mold image in response to determining that the current time meets the preset first interval duration condition. Thus, images of the meniscus transparent mold in the injection molding process can be obtained at intervals, and the method can be used for observing the injection molding condition of the meniscus hydrogel material in the injection molding process. Then, defect detection processing is carried out on the first meniscus transparent mold image, so that defect detection information is obtained. Therefore, whether the underinjection phenomenon occurs in the injection molding process can be determined, and the underinjection phenomenon can be found in time. And then, in response to determining that the defect detection information meets a preset detection condition, determining defect area information according to the defect detection information. Therefore, when the underinjection phenomenon exists in the injection molding process, the area of the underinjection area can be determined, and the method can be used for timely compensating the underinjection area. Then, adjustment angle information is generated based on the defect detection information. And then inputting the defect area information into a preset adjustment information generation model to obtain the adjustment information corresponding to the die adjustment assembly. Wherein, the adjusting information comprises an adjusting speed and an adjusting duration. Therefore, the related information of the adjusting assembly of the adjusting die can be obtained, and the adjusting assembly of the adjusting die can be used for adjusting the adjusting assembly of the die, so that the phenomenon of underfilling is reduced. And finally, controlling the mold adjusting assembly to adjust the meniscus transparent mold according to the adjusting speed, the adjusting angle information and the adjusting time length. Therefore, the angle of the meniscus transparent mold can be adjusted in the injection molding process, the hydrogel material is promoted to flow towards the underinjected area, and the underinjected phenomenon can be reduced. Also because through the regular monitoring injection molding process, can in time discover the phenomenon of underinjecting to when the phenomenon of underinjecting appears, can be according to the regional angle of adjusting meniscus transparent mold through the mould adjusting part, and then reduce the phenomenon of underinjecting, from this, can improve the quality of meniscus.

In step 207, the associated illumination device is controlled to illuminate the tissue engineering meniscus to be coagulated, resulting in a tissue engineering meniscus.

In some embodiments, the executing body may control the associated irradiation device to irradiate the tissue engineering meniscus to be coagulated to obtain the tissue engineering meniscus. The irradiation device may be a device for irradiating the meniscus to be coagulated tissue engineering so as to cure the meniscus. For example, the irradiation device may be a light curing lamp. In practice, the execution body may control the associated irradiation device to irradiate the tissue engineering meniscus to be coagulated for a preset period of time, so as to obtain the tissue engineering meniscus. The preset duration may be a preset duration. For example, the preset time period may be 2 hours.

In the process of solving the technical problems mentioned in the background art by adopting the technical scheme, the following problems are often accompanied: how to determine whether the manufactured meniscus is fixedly molded. For these problems, conventional solutions are generally: the length of time for the meniscus fixation molding is determined according to the experience of the preparation personnel, who then observe whether the meniscus is molded or not by human eyes at the determined time point. However, the above solution has the following technical problem four: whether the meniscus is molded or not is observed through human eyes, at the end stage of solidification of the hydrogel material, the state change generated by the hydrogel material is smaller, the accuracy of human eyes is lower, the quality of the manufactured meniscus is lower when the molding is judged manually and the meniscus is not molded actually, and the irradiation equipment occupies longer time when the molding is judged manually and the meniscus is not molded actually, so that the waste of the irradiation equipment is caused. Thus, considering the need for a user to have high meniscus quality requirements during meniscus use and to maximize illumination device utilization during fabrication, the following solution is decided to be adopted:

In some optional implementations of some embodiments, the executing body may control the associated irradiation device to irradiate the tissue engineering meniscus to be coagulated to obtain the tissue engineering meniscus by:

a first step of controlling the above-described irradiation apparatus to perform an irradiation on operation in response to receiving the irradiation on information, and performing the following detection steps:

and a first detection step, wherein the image acquisition component is controlled to acquire a second meniscus transparent mold image in response to the current time meeting the condition of a preset second interval duration. Wherein the irradiation start information may characterize the start of the irradiation device. The above-described irradiation turning-on operation may be an operation of turning on the irradiation apparatus. The preset second interval duration condition may be that an interval duration between the current time and a time of starting the irradiation device is a preset second interval duration, or an interval duration between the current time and a last time of acquiring the second meniscus transparent mold image is a preset third interval duration. The preset second interval duration may be a duration required for shaping the meniscus of the tissue engineering to be coagulated, which is preset empirically. For example, the preset second interval duration may be 2 hours. The preset third interval duration may be a preset interval duration for detecting whether the meniscus of the tissue engineering to be coagulated is formed or not each time. For example, the preset third interval period may be 5 minutes. The second meniscus transparent mold image described above may be an image characterizing a meniscus transparent mold filled with the tissue engineering meniscus to be coagulated.

And a second detection step, controlling the mould adjusting component to adjust the meniscus transparent mould according to a preset adjusting information sequence. The preset adjustment information sequence may be a sequence in which each preset adjustment information is arranged according to an adjustment sequence. The preset adjustment information may be information of adjusting the adjustable bracket set in advance. The preset adjustment information may include, but is not limited to, a preset adjustment direction, a preset adjustment angle, and a preset adjustment speed. The preset adjustment direction may be a preset adjustment direction. The preset adjustment angle may be a preset adjustment angle. The preset adjustment speed may be a preset adjustment speed. In practice, the execution body may sequentially control the adjustable support included in the mold adjusting assembly for each preset adjusting information included in the preset adjusting information sequence, and adjust the meniscus transparent mold to a position corresponding to the preset adjusting angle included in the preset adjusting information according to the preset adjusting speed included in the preset adjusting information and the preset adjusting direction included in the preset adjusting information.

And a third detection step of controlling the image acquisition component to acquire a third meniscus transparent mold image. The third meniscus transparent mold image may be an image of a meniscus transparent mold after adjusting the meniscus transparent mold.

And a fourth detection step, namely carrying out texture feature extraction processing on the second meniscus transparent mold image to obtain first texture feature information. In practice, the execution body may perform texture feature extraction processing on the second meniscus transparent mold image through a preset texture feature extraction algorithm, so as to obtain first texture feature information. The preset texture feature extraction algorithm may be a preset algorithm for extracting texture features. The preset texture feature extraction algorithm may be, but is not limited to, one of the following: gray level co-occurrence matrix algorithm, and texture feature extraction algorithm based on neural network.

And fifth detection, namely carrying out texture feature extraction processing on the third meniscus transparent mold image to obtain second texture feature information. In practice, the execution body may perform texture feature extraction processing on the third meniscus transparent mold image through the preset texture feature extraction algorithm, so as to obtain second texture feature information.

And a sixth detection step, carrying out matching processing on the first texture feature information and the second texture feature information to obtain matching information. In practice, the executing body may determine, according to a preset similarity algorithm, feature similarities of the first texture feature information and the second texture feature information as the matching information. The preset similarity algorithm may be a preset algorithm for solving the similarity. For example, the predetermined similarity algorithm may be cosine similarity.

And a seventh detection step of continuing to execute the detection step in response to determining that the matching information does not satisfy a preset matching condition. The preset matching condition may be that the feature similarity represented by the matching information is greater than or equal to a preset similarity. The preset similarity may be a preset similarity representing that two images are identical.

An eighth detection step of controlling the irradiation device to perform an irradiation closing operation and controlling an associated sound playing device to play preset irradiation completion information in response to determining that the matching information satisfies the preset matching condition. Wherein the above-described irradiation closing operation may be an operation of closing the irradiation apparatus. The sound playing device may be a speaker. The preset irradiation completion information may be a preset voice indicating completion of irradiation. For example, the preset irradiation completion information may be a voice of "irradiation completed".

The technical solution and related matters are taken as an invention point of the embodiments of the present disclosure, and the fourth technical problem mentioned in the background art is solved, that is, whether the meniscus is molded or not is observed by human eyes, at the end stage of solidification of the hydrogel material, the state change generated by the hydrogel material is smaller, the accuracy of human eye observation is lower, when the molding is manually judged to be actually not molded, the quality of the manufactured meniscus is lower, when the molding is manually judged to be not molded to be actually molded, the time occupied by the irradiation equipment is longer, and the waste of the irradiation equipment is caused. Factors that lead to lower quality of the manufactured meniscus or waste of irradiation equipment tend to be as follows: whether the meniscus is molded or not is observed through human eyes, at the end stage of solidification of the hydrogel material, the state change generated by the hydrogel material is smaller, the accuracy of human eyes is lower, the quality of the manufactured meniscus is lower when the molding is judged manually and the meniscus is not molded actually, and the irradiation equipment occupies longer time when the molding is judged manually and the meniscus is not molded actually, so that the waste of the irradiation equipment is caused. If the above factors are solved, the effects of improving the quality of meniscus and reducing the waste of irradiation equipment can be achieved. To achieve this effect, the tissue engineering meniscus manufacturing method of the present disclosure first controls the above-described irradiation apparatus to perform an irradiation on operation in response to receiving the irradiation on information, and performs the following detection steps: first, in response to the current time meeting a preset second interval duration condition, the image acquisition component is controlled to acquire a second meniscus transparent mold image. Thus, an image of the meniscus transparent mold at the current time can be acquired, which can be used to determine whether the tissue engineering meniscus to be coagulated is coagulated and formed. And secondly, controlling the mould adjusting component to adjust the meniscus transparent mould according to a preset adjusting information sequence. Thus, the meniscus transparent mold can be adjusted so that some flow of uncured meniscus hydrogel material can be created. Then, the image acquisition assembly is controlled to acquire a third meniscus transparent mold image. Thus, an adjusted meniscus transparent mold image can be obtained. And then, carrying out texture feature extraction processing on the second meniscus transparent mold image to obtain first texture feature information. And carrying out texture feature extraction processing on the third meniscus transparent mold image to obtain second texture feature information. Thus, individual image texture features of the meniscus transparent mold image, which adjust the front and back of the meniscus mold, can be obtained and used to determine whether the tissue engineering meniscus to be coagulated includes a meniscus hydrogel material that is completely coagulated. And then, carrying out matching processing on the first texture feature information and the second texture feature information to obtain matching information. Thus, it can be known whether the individual image texture features of the meniscus transparent mold images before and after adjustment of the meniscus mold match, and thus can be used to determine whether the tissue engineering meniscus to be coagulated includes a meniscus hydrogel material that is completely coagulated. And then, in response to determining that the matching information does not meet the preset matching condition, continuing to execute the detection step. Thus, when the tissue engineering meniscus to be coagulated includes a meniscus hydrogel material that is not completely coagulated, the detection is continued. Finally, in response to determining that the matching information meets the preset matching condition, controlling the irradiation device to execute irradiation closing operation, and controlling the associated sound playing device to play preset irradiation completion information. When it is determined from the acquired meniscus mold image that the tissue engineering meniscus to be coagulated includes a meniscus hydrogel material that is completely coagulated, irradiation may be automatically stopped and the preparation personnel prompted. Also because when determining whether the tissue engineering meniscus made of the meniscus hydrogel material is fixed and molded, the transparent meniscus mold can be adjusted by setting the detected interval time length and then detecting the tiny flow change which is not easy to be observed by human eyes through image analysis, thereby improving the accuracy of judging whether the tissue engineering meniscus to be solidified is completely solidified, improving the quality of the meniscus and reducing the waste of irradiation equipment.

The above embodiments of the present disclosure have the following advantageous effects: by the tissue engineering meniscus manufacturing method of some embodiments of the present disclosure, the applicability of the tissue engineering meniscus may be improved. In particular, the lower applicability of menisci is caused by: the hydrogel material is printed in a 3D printing mode, when the printability of the hydrogel material is low, the accuracy of the shape and the size of the tissue engineering meniscus obtained by printing is low, and therefore the applicability of the tissue engineering meniscus is low. Based on this, the tissue engineering meniscus manufacturing method of some embodiments of the present disclosure, first, meniscus image data is acquired. Thus, image data of the meniscus to be repaired can be obtained, so that a personalized meniscus can be produced. And generating meniscus point cloud data according to the meniscus image data. Thus, three-dimensional data of the meniscus can be obtained, which can be used to generate a meniscus model. Then, a meniscus curved surface model is generated according to the meniscus point cloud data. Thus, a three-dimensional model of the meniscus can be obtained, and can be used to generate a three-dimensional model of a mold for producing the meniscus. And then, generating a meniscus mould three-dimensional model according to the meniscus curved surface model. Thus, a three-dimensional model of a meniscus mold having a high degree of adhesion to the meniscus to be repaired can be obtained, and the model can be used for manufacturing a meniscus mold. Next, the associated mold manufacturing apparatus is controlled to manufacture a meniscus transparent mold based on the meniscus mold three-dimensional model. Thus, a transparent mold for manufacturing a meniscus can be obtained, which can be used for manufacturing a meniscus and facilitate observation of the process of manufacturing a meniscus. Next, the associated meniscus injection equipment is controlled to inject the preformed meniscus hydrogel material into the meniscus transparent mold described above, resulting in a tissue engineering meniscus to be solidified. Thus, the pre-prepared meniscus hydrogel material can be injected into the mold through an injection molding process, so that the accuracy of the shape and size of the manufactured meniscus can be improved. Finally, controlling the associated irradiation equipment to irradiate the tissue engineering meniscus to be coagulated, so as to obtain the tissue engineering meniscus. Thus, a tissue engineering meniscus with high shape and size fitting degree can be obtained, and the applicability of the tissue engineering meniscus can be improved. Also, because when the tissue engineering meniscus is manufactured, firstly a meniscus mold having a high degree of adhesion with the meniscus to be repaired is manufactured, and then the meniscus hydrogel material having poor printability is injected into the meniscus mold through an injection molding process, the degree of adhesion of the tissue engineering meniscus with the meniscus to be repaired is improved, and thus, the applicability of the tissue engineering meniscus can be improved.

The present disclosure also provides a tissue engineering meniscus. The tissue engineering meniscus is prepared by the steps 201-207 described above.

The foregoing description is only of the preferred embodiments of the present disclosure and description of the principles of the technology being employed. It will be appreciated by those skilled in the art that the scope of the invention in the embodiments of the present disclosure is not limited to the specific combination of the above technical features, but encompasses other technical features formed by any combination of the above technical features or their equivalents without departing from the spirit of the invention. Such as the above-described features, are mutually substituted with (but not limited to) the features having similar functions disclosed in the embodiments of the present disclosure.

Claims

1. A method of preparing a meniscus hydrogel material, comprising:

mixing an acryloylglycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator and water to obtain a mixed solution, wherein the mixing of the acryloylglycine monomer, chitosan, the methylene bisacrylamide aqueous solution, the photoinitiator and water to obtain the mixed solution comprises the following steps:

Mixing an acryloylglycine monomer, chitosan, a methylene bisacrylamide aqueous solution, a photoinitiator and water through an oscillator to obtain a mixed solution, wherein the content range of the acryloylglycine monomer is 0.1-10 g, the mass fraction range of the chitosan is 0.2-10%, the concentration range of the methylene bisacrylamide aqueous solution is 5-20 mg/ml, and the mass fraction of the photoinitiator is 0.1-1%;

heating the mixed solution to obtain a meniscus hydrogel composite solution;

and curing the meniscus hydrogel composite solution to obtain a meniscus hydrogel material.

2. A method of tissue engineering meniscus fabrication comprising:

acquiring meniscus image data;

generating meniscus point cloud data according to the meniscus image data;

generating a meniscus curved surface model according to the meniscus point cloud data;

generating a meniscus mould three-dimensional model according to the meniscus curved surface model;

controlling an associated mold-making device to make a meniscus transparent mold from the meniscus mold three-dimensional model;

controlling an associated meniscus injection device to inject a preformed meniscus hydrogel material into the meniscus transparent mould to obtain a meniscus for tissue engineering to be solidified, wherein the preformed meniscus hydrogel material is prepared by the meniscus hydrogel material preparation method according to claim 1;

And controlling the associated irradiation equipment to irradiate the tissue engineering meniscus to be coagulated to obtain the tissue engineering meniscus.

3. The method of claim 2, wherein the generating a three-dimensional model of a meniscus mold from the meniscus curved surface model comprises:

shearing the meniscus curved surface model to obtain a meniscus model with a vertical section, an upper intersecting line and a lower intersecting line;

generating a meniscus column model according to the meniscus curved surface model and the meniscus model with the vertical section;

parting treatment is carried out on the meniscus columnar body model according to the upper intersecting line and the lower intersecting line, so as to obtain a meniscus upper die model and a meniscus lower die model;

the upper meniscus mold model and the lower meniscus mold model are determined as a three-dimensional model of a meniscus mold.

4. The method of claim 2, wherein the mold manufacturing apparatus comprises a machining device, an electrochemical corrosion device, and a meniscus mold injection device; and

the control-associated mold manufacturing apparatus manufactures a meniscus transparent mold from the meniscus mold three-dimensional model, comprising:

controlling the machining equipment to process according to the meniscus mould three-dimensional model to obtain a meniscus mould male mould;

Controlling the electrochemical corrosion equipment to perform electrochemical corrosion treatment on the meniscus mould male die to obtain a meniscus mould cavity;

and controlling the meniscus mold injection equipment to inject the prefabricated transparent injection molding material into the meniscus mold cavity to obtain the meniscus transparent mold.

5. The method of claim 2, wherein the meniscus transparent mold comprises an upper mold and a lower mold, the upper mold and the lower mold mating.

6. The method of claim 5, wherein the upper surface of the upper mold is further provided with a gate.

7. The method of claim 6, wherein the upper surface of the upper mold is further provided with risers.

8. The method of claim 5, wherein a detent is provided in the upper die and a detent post is provided on an upper surface of the lower die, the detent being an interference fit with the detent post.

9. A tissue engineering meniscus, wherein the tissue engineering meniscus is prepared by the tissue engineering meniscus manufacturing method according to any one of claims 2 to 8.