CN117535130A - Detection device and method thereof - Google Patents
Detection device and method thereof Download PDFInfo
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- CN117535130A CN117535130A CN202311426910.1A CN202311426910A CN117535130A CN 117535130 A CN117535130 A CN 117535130A CN 202311426910 A CN202311426910 A CN 202311426910A CN 117535130 A CN117535130 A CN 117535130A
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- C—CHEMISTRY; METALLURGY
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of biochemical detection, and particularly discloses a detection device and a detection method. The detection device comprises a processing tube, an amplification tube, a dilution tube, a detection box, a first communication mechanism, a second communication mechanism and a third communication mechanism. Wherein the amplification tube is connected with the treatment tube; the dilution tube is connected with the amplification tube; the detection box is connected with the amplification tube or the dilution tube; the treatment tube can be communicated with the inner cavity of the amplification tube through the first communication mechanism; the amplification tube can be communicated with the inner cavity of the dilution tube through a second communication mechanism; the dilution pipe can be communicated with the inner cavity of the detection box through a third communication mechanism; alternatively, the amplification tube may be in communication with the cartridge cavity via a third communication mechanism. After the sample to be tested is put into the amplification tube to start reaction, the intermediate process is not required to be operated by using a dropper, the sample is not required to be exposed to the air, the introduction of pollutants is avoided to the greatest extent, the accuracy of the test result is increased, the operation method is optimized, the steps are simple, and the sample is prevented from being scattered.
Description
Technical Field
The invention relates to the technical field of biochemical detection, in particular to a detection device and a detection method.
Background
Nucleic acid is used as biological genetic information carrier, different base sequences represent different genetic information of organisms, and the nucleic acid sequence is specifically detected to effectively identify the object to be detected. Currently, nucleic acid detection has been widely used in various fields such as clinical diagnosis, medical research, environmental monitoring, food safety, criminal investigation and identification.
Nucleic acid detection processes typically include nucleic acid sample processing, nucleic acid amplification, concentration dilution, and product analysis, which typically require detection by specialized inspectors in molecular laboratories relying on sophisticated detection instruments. With the development of technology, people begin to use a portable nucleic acid kit for home detection, and although the content of the target sequence nucleic acid cannot be accurately analyzed, whether the target sequence nucleic acid exists or not can be determined with high probability, and the daily detection requirement can be basically met.
At present, when a nucleic acid kit is used for detection, an initial sample needs to be treated, for example, nucleic acid extraction, after the treatment is finished, a sample to be detected is put into an amplification reagent by a dropper for amplification, after the amplification of the sample to be detected is finished, the sample is sucked out by the dropper and is dripped into a diluent for dilution, and after the dilution of the sample is finished, the sample is sucked out by the dropper and is dripped onto test paper for detection. In the process of sucking and dripping a sample by using a dropper, the sample liquid is directly exposed in the air, so that pollutants are easily introduced, the final test result is inaccurate, the operation process is complicated, and faults such as dripping easily occur.
Disclosure of Invention
In view of the above, the invention provides a detection device and a method thereof, which are used for solving the problems that pollutants are easily introduced, the operation process is complex and misoperation is easy to occur when the traditional nucleic acid detection kit is used for sucking and dripping a dropper during nucleic acid detection.
In a first aspect, the present invention provides a detection device for nucleic acid detection, comprising a processing tube, an amplification tube, a dilution tube, a detection cartridge, a first communication mechanism, a second communication mechanism, and a third communication mechanism. Wherein the amplification tube is connected with the treatment tube; the dilution tube is connected with the amplification tube; the detection box is connected with the amplification tube or the dilution tube; the treatment tube can be communicated with the inner cavity of the amplification tube through the first communication mechanism; the amplification tube can be communicated with the inner cavity of the dilution tube through a second communication mechanism; the dilution pipe can be communicated with the inner cavity of the detection box through a third communication mechanism; alternatively, the amplification tube may be in communication with the cartridge cavity via a third communication mechanism.
The beneficial effects are that: in the scheme, an amplification tube is connected with a treatment tube; the dilution tube is connected with the amplification tube; the detection box is connected with the amplification tube or the dilution tube; the treatment tube can be communicated with the inner cavity of the amplification tube through the first communication mechanism; the amplification tube can be communicated with the inner cavity of the dilution tube through a second communication mechanism; the dilution pipe can be communicated with the inner cavity of the detection box through a third communication mechanism; the amplification tube can be communicated with the inner cavity of the detection box through a third communication mechanism. Therefore, after the sample to be tested is put into the amplification tube to start reaction, the operation of a dropper is not needed in the middle process, and the sample is not needed to be exposed to the air, so that the introduction of pollutants is avoided to the greatest extent, and the accuracy of the test result is increased; in addition, the scheme optimizes the operation method, has simple steps and avoids faults such as sample scattering and the like.
In an alternative embodiment, the first communication mechanism is a rotational communication mechanism, and the processing tube and the amplification tube rotate relative to each other to communicate the lumens.
The beneficial effects are that: this scheme is the concrete implementation of first coupling mechanism, and after processing pipe and the amplification union coupling, rotate coupling mechanism and make processing pipe and the inner chamber intercommunication of amplification pipe, convenient operation has avoided error such as sample unrestrained, has realized the effect of selecting the time intercommunication moreover.
In an alternative embodiment, the first communication mechanism includes a first seal structure; the first sealing structure is used for sealing a sample outlet of the treatment tube, the sample outlet of the treatment tube is eccentrically arranged relative to the axis of the treatment tube, the treatment tube and the amplification tube are separated from the first sealing structure through relative rotation, and the treatment tube is communicated with the inner cavity of the amplification tube; or the first sealing structure is used for sealing the sample inlet of the amplification tube, the sample inlet of the amplification tube is eccentrically arranged relative to the axis of the amplification tube, the treatment tube and the amplification tube are separated from the sample inlet of the amplification tube and the first sealing structure through relative rotation, and the treatment tube is communicated with the inner cavity of the amplification tube.
The beneficial effects are that: this scheme is further concrete implementation of first coupling mechanism, and first seal structure shutoff is handled the opening that pipe or expansion pipe eccentrically set up, rotates relatively and is handled pipe and expansion pipe, makes first seal mechanism and opening break away from, and then makes to handle pipe and expansion pipe inner chamber intercommunication, realizes the effect of rotation intercommunication, easy operation.
In an alternative embodiment, the lumen of the processing tube is configured to be collapsible such that the sample fluid within the processing tube is accelerated into the amplification tube.
The beneficial effects are that: in the scheme, the inner cavity of the treatment tube can be arranged in a shrinkable manner, so that the sample liquid in the treatment tube can flow into the amplification tube in an accelerating manner, the control on the flow of the sample liquid is improved, and the operation time is saved.
In an alternative embodiment, the treatment tube comprises a first portion and a second portion, the first portion being connected to the second portion in an axially movable, circumferentially limited manner.
The beneficial effects are that: this scheme is the concrete implementation of processing pipe, and processing pipe includes first portion and second portion, and first portion and second portion are connected with axial movable, the spacing mode of circumference, and wherein first portion and second portion axial movable realize the contractible effect of processing pipe inner chamber, and first portion and second portion circumference are spacing, realize that both can drive each other and rotate, realize the purpose of rotation intercommunication.
In an alternative embodiment, the sample inlet of the amplification tube comprises a first sample inlet and a second sample inlet; the first sample inlet is communicated with the sample outlet of the treatment tube through a first communication mechanism, and the second sample inlet is communicated with the sample outlet of the dilution tube through a second communication mechanism; the sample outlet of the amplification tube is communicated with the sample inlet of the detection box through a third communication mechanism.
The beneficial effects are that: this scheme is the concrete implementation who handles between pipe, the amplification pipe, dilution pipe and the detection box, and two sample inlets of amplification pipe communicate with the sample export of handling pipe and dilution pipe respectively, and the sample export of amplification pipe communicates with the sample inlet of detection box, sets up ingenious, compact structure, has avoided the introduction of pollutant and the unrestrained risk of sample.
In an alternative embodiment, the second communication mechanism comprises a second sealing structure and a first piercing structure adapted to pierce the second sealing structure, the second sealing structure being disposed on one of the second sample inlet and the sample outlet of the dilution tube, the first piercing structure being disposed on the other.
The beneficial effects are that: the scheme is a specific communication mode between the amplification tube and the dilution tube, the second sealing structure seals the opening of the amplification tube or the dilution tube, and the second sealing structure is punctured by the first puncture structure, so that the operation is convenient, the structure is simple, and the purpose of communicating the inner cavities of the amplification tube and the dilution tube is achieved.
In an alternative embodiment, the second sealing structure is disposed at the sample outlet of the dilution tube, the first puncturing structure is disposed at the second sample inlet, and the second communication mechanism further includes a driving member for driving the first puncturing structure to move upward and puncture the second sealing structure.
The beneficial effects are that: the scheme is a specific puncturing mode of the first puncturing structure, and the driving piece is used for driving the first puncturing structure to move upwards and puncture the second sealing structure, so that the purpose of communicating the dilution tube with the inner cavity of the amplification tube is achieved.
In an alternative embodiment, the driving member is disposed within the cartridge and the driving member is disposed coaxially with the first piercing structure.
The beneficial effects are that: the specific setting mode of driving piece is this scheme, and the driving piece sets up in the detection box, and driving piece and the coaxial setting of first puncture structure realize the purpose that the first puncture structure of driving piece drive upwards moves and pierces the second seal structure.
In an alternative embodiment, the third communication mechanism comprises a third sealing structure and a second puncturing structure adapted to puncture the third sealing structure, the third sealing structure being disposed on one of the sample outlet of the amplification tube and the sample inlet of the cartridge, the second puncturing structure being disposed on the other.
The beneficial effects are that: this scheme is the concrete implementation of third intercommunication mechanism, and third seal structure seals locates amplification pipe or detection box, and second puncture structure pierces third seal structure and makes amplification pipe and detection box inner chamber intercommunication, convenient operation has avoided risk such as sample unrestrained.
In an alternative embodiment, the third sealing structure is disposed at the sample outlet of the amplification tube, the second puncturing structure is disposed at the sample inlet of the detection cartridge, and the height of the second puncturing structure is lower than the height of the driving member.
The beneficial effects are that: the scheme is a specific implementation mode of a second puncture structure, the height of the second puncture structure is lower than that of a driving piece, and in the butt joint process of an amplification tube and a detection box, the purpose that the driving piece jacks up the first puncture structure to puncture a second sealing structure and then the second puncture structure to puncture a third sealing structure is achieved.
In an alternative embodiment, the processing tube is above the amplification tube with the dilution tube and the processing tube is juxtaposed with the dilution tube.
The beneficial effects are that: the scheme is a specific setting mode among the treatment tube, the dilution tube and the amplification tube, the treatment tube and the dilution tube are arranged above the amplification tube, and the treatment tube and the dilution tube are arranged side by side, so that the device is compact in structure and small in occupied space.
In an alternative embodiment, the test cassette is provided with at least one test strip extending in the height direction of the test device, the test end of the test strip being provided at the sample inlet of the test cassette.
The beneficial effects are that: this scheme is the concrete setting mode of test paper, and the detection box is equipped with at least one test paper, and the test paper extends along detection device's direction of height, and the sample entry of detection box is located to the detection end of test paper, compact structure reduces the volume of detection box.
In a second aspect, the present invention provides a detection method, using the detection device of the first aspect, for nucleic acid detection, comprising the steps of:
placing an initial sample into a treatment tube from a sample inlet of the treatment tube, sealing the sample inlet of the treatment tube, and treating the initial sample by a preset reagent in the treatment tube;
after the initial sample is processed, the processing tube is rotated to separate the first sealing structure from the sample outlet of the processing tube, so that the processing tube is communicated with the inner cavity of the amplification tube;
the treated sample flows into an amplification tube, and a preset reagent in the amplification tube is used for amplifying the sample;
after the sample is amplified, a driving piece on the detection box drives the first puncture structure to move upwards and puncture the second sealing structure, and a reagent preset in the dilution pipe flows into the amplification pipe and dilutes the amplified sample;
after the sample is diluted, the second puncture structure of the detection box punctures the third sealing structure of the sample outlet of the amplification tube, and diluted sample liquid flows into the sample inlet of the detection box and performs chromatographic reaction through test paper.
The beneficial effects are that: the detection device of the first aspect has all the beneficial effects of the detection device; in addition, the detection method is convenient to operate and is beneficial to improving the detection efficiency.
In an alternative embodiment, the step of "the treated sample flows into an amplification tube, and the preset reagent in the amplification tube amplifies the sample" includes:
pressing the first or second portion of the processing tube causes the lumen of the processing tube to contract, and the processed sample accelerates into the amplification tube.
The beneficial effects are that: the technical scheme is that the amplification step of the processed sample is further optimized, the first part or the second part of the processing tube is pressed, the inner cavity of the processing tube is contracted, the processed sample flows into the amplification tube in an accelerating way, the flow speed of the sample is improved, and the detection efficiency is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the overall structure of a detection device according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a detection device according to an embodiment of the present invention;
FIG. 3 is an exploded view of a detection device according to an embodiment of the present invention;
FIG. 4 is a cross-sectional view of FIG. 3;
FIG. 5 is a schematic diagram illustrating an assembly of an external structure and a connection structure according to an embodiment of the present invention;
FIG. 6 is a first isometric view of a connection structure according to an embodiment of the present invention;
FIG. 7 is a second isometric view of a connecting structure according to an embodiment of the present invention;
FIG. 8 is a third isometric view of a connecting structure according to an embodiment of the present invention;
FIG. 9 is a first isometric view of an outer structure according to an embodiment of the present invention;
FIG. 10 is a second isometric view of an outer structure according to an embodiment of the present invention;
FIG. 11 is a third isometric view of an outer structure according to an embodiment of the present invention;
FIG. 12 is a schematic view of a first puncture structure according to an embodiment of the present invention;
FIG. 13 is a schematic diagram illustrating the assembly of the external structure and the cartridge according to the embodiment of the present invention;
FIG. 14 is a first isometric view of a cartridge according to an embodiment of the present invention;
FIG. 15 is a second isometric view of a cartridge according to an embodiment of the present invention;
fig. 16 is a flowchart illustrating an operation of the detecting device according to the embodiment of the invention.
Reference numerals illustrate:
100. a detection device;
110. a treatment tube; 120. an amplification tube; 130. a dilution tube; 140. a detection box; 150. a connection structure;
111. A first portion; 112. a second portion; 113. a first seal ring; 114. a second seal ring; 115. a sealing plug;
1111. a cover body; 1112. a first clamping structure;
1121. a second clamping structure;
121. an outer structure; 122. a first diversion ramp; 123. a second diversion ramp; 124. a limit structure;
131. a first piercing structure;
1311. a sharp end; 1312. a plugging plate; 1313. carrying out push rod waiting; 1314. a blocking part;
141. a bottom support plate; 142. a baffle; 143. a driving member; 144. a second piercing structure; 145. a clamping groove; 146. test paper;
1411. anti-skid lines;
151. a third seal ring; 152. a fourth seal ring; 153. a bracket; 154. a limit groove; 155. a connecting pipe;
1551. a guide groove.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order to enhance the understanding of the solution of the present invention, the technical problems need to be elaborated in combination with the related technologies, and the specific contents are as follows:
when the nucleic acid kit is used for detection, a sample to be detected needs to be put into an amplification reagent for amplification, the sample to be detected is sucked out by a dropper and dripped into a diluent for dilution after the amplification of the sample to be detected is finished, and the sample is sucked out by the dropper and dripped onto test paper for detection after the dilution of the sample is finished.
For easy understanding, the above procedure may refer to antigen detection steps that residents routinely contact, and in contrast to nucleic acid detection, antigen detection does not require amplification of a sample to be tested, and antigen detection generally detects the protein coat of a virus, whereas nucleic acid detection detects genetic material, specifically DNA or RNA, inside the virus coat.
In the operation process, a dropper is required to be used for sucking and dripping a sample, the sample liquid is directly exposed in the air, pollutants are easily introduced, the final test result is inaccurate, the operation process is complicated, and faults such as spilling and dripping easily occur.
Further, such nucleic acid detection is generally performed in a residential room, where the air fluidity is poor, and virus samples can diffuse into the air, which is liable to cause the risk of aerosol infection; in addition, the condition of a sterile laboratory is not provided in the resident room, so that a detection sample is easy to pollute, and the detection of the nucleic acid sample is false positive and influences the detection result.
In order to alleviate the above-mentioned technical problems, the present invention provides a detection device and a method thereof, and embodiments of the present invention are described in detail below with reference to fig. 1 to 16.
First, referring to FIGS. 1 to 4, the present invention provides a detection device 100 for detecting nucleic acid, which comprises a processing tube 110, an amplification tube 120, a dilution tube 130, a detection cartridge 140, a first communication mechanism, a second communication mechanism, and a third communication mechanism. Wherein the amplification tube 120 is connected to the processing tube 110; the dilution tube 130 is connected to the amplification tube 120; the cartridge 140 is connected to the amplification tube 120 or the dilution tube 130; the treatment tube 110 can be communicated with the inner cavity of the amplification tube 120 through a first communication mechanism; the amplification tube can be communicated with the inner cavity of the dilution tube 130 through a second communication mechanism; the dilution tube 130 can be communicated with the inner cavity of the detection box 140 through a third communication mechanism; alternatively, the amplification tube 120 may be in communication with the interior of the cassette 140 via a third communication mechanism.
Specifically, the amplification tube 120 is connected to the treatment tube 110 in this embodiment; the dilution tube 130 is connected to the amplification tube 120; the cartridge 140 is connected to the amplification tube 120 or the dilution tube 130; the treatment tube 110 can be communicated with the inner cavity of the amplification tube 120 through a first communication mechanism; the amplification tube can be communicated with the inner cavity of the dilution tube 130 through a second communication mechanism; the dilution tube 130 can be communicated with the inner cavity of the detection box 140 through a third communication mechanism; the amplification tube 120 can be in communication with the interior of the cartridge 140 via a third communication mechanism. Therefore, after the sample to be tested is put into the amplification tube 120 to start reaction, the operation of a dropper is not needed in the middle process, and the sample is not needed to be exposed to the air, so that the introduction of pollutants is avoided to the greatest extent, and the accuracy of the test result is increased; in addition, the scheme optimizes the operation method, has simple steps and avoids faults such as sample scattering and the like.
Further, this scheme connects and communicates processing tube 110, amplification tube 120, dilution tube 130 and detection box 140, realizes full flow integration setting, accomplishes to add detection device 100 from the original sample and all need not the burette to carry out the pipetting to obtaining the testing result, does not introduce the pollutant and the risk of unrestrained sample.
Preferably, the first communication mechanism and the second communication mechanism may be configured as a time-selective communication structure, so as to better control the communication time, improve the mastering performance of the intermediate process, and the third communication mechanism may be configured as a docking communication mechanism, that is, a docking communication mechanism, and may also be configured as a time-selective communication structure. In addition, the third communication mechanism can connect and communicate the whole formed by the amplification tube 120 and the dilution tube 130 with the test paper 146 box, specifically, the amplification tube 120 or the dilution tube 130 is communicated with the inner cavity of the test paper 146 box.
It should be noted that, the conventional nucleic acid amplification method is mainly polymerase chain reaction (Polymerase Chain Reaction, PCR), and along with the development of technology, some isothermal amplification reactions without complicated temperature control are widely used for nucleic acid amplification, such as Loop-mediated isothermal amplification (Loop-mediated Isothermal Amplification, LAMP), recombinase polymerase amplification (Recombinase Polymerase Amplification, RPA), and the like. The type of isothermal amplification in this scheme may be, but is not limited to, loop-mediated isothermal amplification reactions or recombinant polymerase reactions, and isothermal processes may use water baths or metal baths.
The nucleic acid amplification generally requires a template, a primer, an enzyme, a base and the like, wherein the primer is connected to one end of the template, the paired bases are sequentially connected from the primer under the action of the enzyme, and finally a new nucleic acid substance corresponding to the template is formed, and the nucleic acid is amplified for multiple times, so that a small amount of initial nucleic acid is increased, and the detection sensitivity is increased.
In particular, the reagents involved in the amplification reaction may be in liquid or lyophilized form, and subsequently digested by heating.
In some embodiments, referring to FIG. 3, the first communication mechanism is a rotational communication mechanism, and the processing tube 110 and the amplification tube 120 are rotated relative to each other to communicate the lumens.
Specifically, this scheme is the concrete implementation of first coupling mechanism, and processing tube 110 is connected the back with amplification pipe 120, rotates coupling mechanism and makes processing tube 110 and amplification pipe 120 inner chamber intercommunication, convenient operation has avoided error such as sample unrestrained, has realized the effect of selecting the time intercommunication moreover.
It should be noted that the processing tube 110 is mainly used for operations before performing nucleic acid amplification, including, but not limited to, nucleic acid extraction. Nucleic acid extraction refers to the extraction of DNA or RNA from the nucleus by disrupting the cell membrane of the cell or disrupting the protein coat of the virus by the corresponding extraction enzyme.
In addition, the first communication mechanism is mainly used for releasing the processed sample under specific conditions, and is not limited to a rotation action, but includes other modes such as a pressing action and the like.
In some embodiments, referring to fig. 3 and 6-8, the first communication mechanism includes a first seal structure; the first sealing structure is used for sealing a sample outlet of the processing tube 110, the sample outlet of the processing tube 110 is eccentrically arranged relative to the axis of the processing tube 110, the processing tube 110 and the amplification tube 120 are separated from the first sealing structure through relative rotation, and the processing tube 110 is communicated with the inner cavity of the amplification tube 120; alternatively, the first sealing structure is used for sealing the sample inlet of the amplification tube 120, the sample inlet of the amplification tube 120 is eccentrically arranged relative to the axis of the amplification tube 120, the processing tube 110 and the amplification tube 120 are separated from the sample inlet of the amplification tube 120 by relative rotation, and the processing tube 110 is communicated with the inner cavity of the amplification tube 120.
Specifically, this scheme is further specific implementation of first coupling mechanism, and first seal structure shutoff is handled the opening that pipe 110 or amplification pipe 120 set up eccentrically, rotates relatively and handles pipe 110 and amplification pipe 120, makes first seal mechanism and opening break away from, and then makes handle pipe 110 and amplification pipe 120 inner chamber intercommunication, realizes rotating the effect of intercommunication, easy operation.
More specifically, according to some of the embodiments, the processing tube 110 is connected to the amplification tube 120 through a connection tube 155, a main body portion of the processing tube 110 is provided in the connection tube 155, and one end of the connection tube 155 is connected to the amplification tube 120; the inner wall of the connecting tube 155 extends inward to form a support 153, the support 153 is provided with a limit groove 154, and a sealing plug 115 is arranged in the limit groove 154, and the sealing plug 115 is used for blocking a sample outlet of the processing tube 110.
Further, the present solution is a preferred embodiment of the rotation communication mechanism, and in other embodiments, the present solution may be: one of the pipe orifices of the processing pipe 110 and the amplifying pipe 120 is provided with a sealing film, the other one of the pipe orifices is provided with a puncture structure, and the puncture structure is eccentrically arranged, namely, the pipe orifice of the processing pipe 110 is provided with the sealing film, the pipe orifice of the amplifying pipe 120 is provided with a puncture structure eccentric relative to the axis, or the pipe orifice of the amplifying pipe 120 is provided with the sealing film, and the pipe orifice of the processing pipe 110 is provided with a puncture structure eccentric relative to the axis; the processing tube 110 and the amplification tube 120 are screwed and connected by screw threads, when the processing tube 110 and the amplification tube 120 rotate mutually, the processing tube and the amplification tube are close to each other, and the puncture structure breaks the sealing film, and an arc-shaped opening is left, so that the processing tube 110 is communicated with the inner cavity of the amplification tube 120, and the purpose of rotating communication can be realized. The above embodiments or modifications thereof fall within the scope of the present application.
In some embodiments, referring to FIG. 3, the lumen of the processing tube 110 may be configured to be collapsible such that the sample fluid within the processing tube 110 is accelerated into the amplification tube 120.
Specifically, in this embodiment, the inner cavity of the processing tube 110 is configured to be contractible, so that the sample liquid in the processing tube 110 flows into the amplification tube 120 with an acceleration, thereby improving the control of the flow of the sample liquid and saving the operation time.
Further, when the sample outlet of the processing tube 110 is eccentrically disposed, the nozzle is smaller, and the inner cavity of the processing tube 110 needs to be compressed to increase the flow rate in this solution.
In some embodiments, referring to fig. 2 and 3, the processing tube 110 includes a first portion 111 and a second portion 112, the first portion 111 and the second portion 112 being connected in an axially movable, circumferentially limited manner.
Specifically, the present embodiment is a specific embodiment of the treatment tube 110, where the treatment tube 110 includes a first portion 111 and a second portion 112, and the first portion 111 and the second portion 112 are connected in an axially movable, circumferentially limited manner; wherein the first portion 111 and the second portion 112 are coaxially abutted and can relatively approach or separate from each other along the axial direction for movement, so as to realize the contractible effect of the inner cavity of the treatment tube 110; in addition, the first portion 111 and the second portion 112 are limited in the circumferential direction, that is, they cannot rotate relative to each other in the circumferential direction, so that the two portions can rotate with each other, and the purpose of rotational communication is achieved.
More specifically, according to some of these embodiments, one end of the first portion 111 protrudes out of the connecting tube 155 and is provided with a sample inlet and with a cover 1111 for placing the initial sample; the second end of the first part 111 is provided with a first clamping structure 1112, and the outer wall of the first part 111 is sleeved with a first sealing ring 113 and is movably sealed in the connecting pipe 155 through the first sealing ring 113; the second clamping structure 1121 is arranged at one end of the second part 112 facing the first part 111, the second clamping structure 1121 is clamped with the first clamping structure 1112, and the second clamping structure and the first clamping structure are circumferentially limited, so that the second part 112 is driven to rotate when the first part 111 is manually rotated; the second clamping structure 1121 is not tightly abutted with the first clamping structure 1112, and a gap is formed between the second clamping structure 1121 and the first clamping structure 1112, namely the first part 111 can move towards the second part 112, the outer wall of the second part 112 is fixedly sealed in the connecting pipe 155 through the second sealing ring 114, the other end of the second part 112 is provided with an eccentric sample outlet, the first part 111 is rotated to drive the second part 112 to rotate, so that the sample outlet is separated from the sealing plug 115, and the treated sample liquid flows out; as the first portion 111 moves toward the second portion 112, the lumen of the processing tube 110 is compressed and the sample fluid accelerates out.
Further, the cover 1111 of the sample inlet of the processing tube 110 may be a folded cover connected to the processing tube 110, or may be a cover 1111 independently provided, including but not limited to a threaded connection, a snap connection, an interference fastening, or an aluminum film heat sealing.
Preferably, the inner wall of the connection tube 155 is provided with a guide groove 1551 engaged with the first part 111, and the guide groove 1551 facilitates the rotation and axial movement of the first part 111.
In some embodiments, referring to fig. 5-11, the sample inlet of the amplification tube 120 comprises a first sample inlet and a second sample inlet; the first sample inlet communicates with the sample outlet of the processing tube 110 through the first communication mechanism, and the second sample inlet communicates with the sample outlet of the dilution tube 130 through the second communication mechanism; the sample outlet of the amplification tube 120 communicates with the sample inlet of the cartridge 140 through a third communication mechanism.
Specifically, the present embodiment is a specific embodiment among the processing tube 110, the amplifying tube 120, the diluting tube 130 and the detection box 140, wherein two sample inlets of the amplifying tube 120 are respectively communicated with sample outlets of the processing tube 110 and the diluting tube 130, and a sample outlet of the amplifying tube 120 is communicated with a sample inlet of the detection box 140, so that the device is smart in arrangement and compact in structure, and the risks of introducing pollutants and scattering samples are avoided.
The purpose of the exponential amplification of the nucleic acid sample is to amplify the number of target nucleic acids and increase the sensitivity of detection; the purpose of diluting the amplified sample is to render the viscous sample fluid more fluid, facilitating the flow of the sample fluid into the cartridge 140 and onto the test strip 146.
In particular, the number of samples after dilution is greater than the number of samples before amplification.
In some embodiments, referring to fig. 8 to 13, the second communication mechanism includes a second sealing structure and a first piercing structure 131 adapted to pierce the second sealing structure, the second sealing structure is disposed on one of the second sample inlet and the sample outlet of the dilution tube 130, the first piercing structure 131 is disposed on the other, i.e., the second sealing structure plugs the second sample inlet, the first piercing structure 131 is disposed near the sample outlet of the dilution tube 130, or the second sealing structure plugs the sample outlet of the dilution tube 130, and the first piercing structure 131 is disposed near the second sample inlet.
Specifically, the present embodiment is a specific communication manner between the amplification tube 120 and the dilution tube 130, and the second sealing structure is sealed at the opening of the amplification tube 120 or the dilution tube 130 and is pierced by the first piercing structure 131, so that the operation is convenient, the structure is simple, and the purpose of communicating the inner cavities of the two is achieved.
In particular, according to various embodiments, the dilution tube 130 may be used mainly for storing a dilution liquid, and after the amplification, the dilution liquid flows into the amplification tube 120 for dilution, and the dilution tube 130 may also be used for diluting a sample liquid flowing in the amplification tube 120.
In some embodiments, referring to fig. 11 to 14, the second sealing structure is disposed at the sample outlet of the dilution tube 130, the first puncturing structure 131 is disposed at the second sample inlet, and the second communication mechanism further includes a driving member 143, where the driving member 143 is configured to drive the first puncturing structure 131 to move upward and puncture the second sealing structure.
Specifically, the present embodiment is a specific puncturing manner of the first puncturing structure 131, and the driving member 143 is configured to drive the first puncturing structure 131 to move upward and puncture the second sealing structure, so as to achieve the purpose of communicating the dilution tube 130 with the inner cavity of the amplification tube 120.
In some embodiments, the driver 143 is disposed within the cartridge 140, and the driver 143 is disposed coaxially with the first piercing structure 131.
Specifically, the present embodiment is a specific setting mode of the driving member 143, where the driving member 143 is disposed in the detection box 140, and the driving member 143 is coaxially disposed with the first piercing structure 131, so as to achieve the purpose that the driving member 143 drives the first piercing structure 131 to move upwards and pierce the second sealing structure.
More specifically, an integrally formed outer structure 121 is arranged outside the amplification tube 120, a second diversion inclined plane 123 is arranged at a part of the outer structure 121 corresponding to the dilution tube 130, and an opening at the bottom of the second diversion inclined plane 123 is communicated with the amplification tube 120; one end of the first puncture structure 131 penetrating through the second diversion inclined plane 123 is a sharp end 1311, and the sharp end 1311 is provided with a plugging plate 1312 for plugging the bottom opening of the diversion inclined plane; the portion of the first puncture structure 131 located at the bottom of the second diversion slope 123 is a to-be-pushed rod 1313, and the to-be-pushed rod 1313 corresponds to the driving piece 143.
Further, according to some embodiments, the driving member 143 and the second piercing structure 144 extend from the bottom of the detection cartridge 140 along the height direction of the detection cartridge 140, respectively, and the height of the driving member 143 is greater than that of the second piercing structure 144, so that when the detection cartridge 140 is docked with the amplification tube 120, the driving member 143 pushes up the first piercing structure 131 to pierce the second sealing structure, thereby realizing that the diluent flows into the amplification tube 120 for dilution, and after the dilution is completed, the amplification tube 120 is moved downwards, and the second piercing structure 144 pierces the third sealing structure, thereby realizing that the sample liquid flows into the detection cartridge 140 for detection.
Preferably, the sharp end 1311 is a cross structure, the top is a blade, the top of the plugging plate 1312 is also a blade, an integrally formed plugging portion 1314 is provided between the sharp end 1311 and the to-be-ejected rod 1313, and the plugging portion 1314 plugs the opening on the second diversion slope 123 for the first puncture structure 131 to move up and down, so as to prevent a great amount of diluent from flowing from the opening on the second diversion slope 123 to the detection box 140 after puncture.
In some embodiments, referring to fig. 9 and 14, the third communication mechanism includes a third sealing structure and a second puncturing structure 144 adapted to puncture the third sealing structure, the third sealing structure is disposed on one of the sample outlet of the amplification tube 120 and the sample inlet of the detection cartridge 140, the second puncturing structure 144 is disposed on the other, i.e., the third sealing structure is blocked at the sample outlet of the amplification tube 120, the second puncturing structure 144 is disposed near the sample inlet of the detection cartridge 140, or the third sealing structure is blocked at the sample inlet of the detection cartridge 140, and the second puncturing structure is disposed near the sample outlet of the amplification tube 120.
Specifically, this scheme is the concrete implementation mode of third intercommunication mechanism, and the third seal structure seals locates amplification tube 120 or detection box 140, and second puncture structure 144 pierces third seal structure and makes amplification tube 120 and detection box 140 inner chamber intercommunication, convenient operation has avoided risk such as sample unrestrained.
In some embodiments, referring to fig. 14, a third sealing structure is disposed at the sample outlet of the amplification tube 120, a second puncturing structure 144 is disposed at the sample inlet of the cartridge 140, and the height of the second puncturing structure 144 is lower than the height of the driving member 143.
Specifically, the present embodiment is a specific implementation manner of the second piercing structure 144, where the height of the second piercing structure 144 is lower than that of the driving member 143, and in the process of docking the amplification tube 120 with the detection box 140, the purpose that the driving member 143 lifts the first piercing structure 131 to pierce the second sealing structure and then the second piercing structure 144 pierces the third sealing structure is achieved.
More specifically, the amplification tube 120 can be moved toward the cartridge 140, bringing the driver 143 into contact with the first piercing structure 131 and the second piercing structure 144 piercing the third sealing structure.
In some embodiments, referring to FIG. 8, the processing tube 110 and the dilution tube 130 are above the amplification tube 120, and the processing tube 110 is disposed in parallel with the dilution tube 130.
Specifically, the present embodiment is a specific arrangement mode among the processing tube 110, the dilution tube 130 and the amplification tube 120, where the processing tube 110 and the dilution tube 130 are above the amplification tube 120, and the processing tube 110 and the dilution tube 130 are arranged in parallel, so that the structure is compact and the occupied space is small.
More specifically, according to some of these embodiments, the treatment tube 110 is disposed in parallel with the connection tube 155 and integrally formed, but the lumens thereof are not communicated with each other, and the treatment tube 110 is separated from the connection tube 155 by the connection structure 150; the processing tube 110 extends into the interior of the connecting tube 155 from one end of the connecting tube 155, and the other end of the connecting tube 155 is sleeved with a third sealing ring 151 and is in sealing connection with the first sample inlet of the amplification tube 120; the top end of the dilution tube 130 is closed, the other end of the dilution tube 130 is provided with a sample outlet with a second sealing structure, preferably an aluminum film, and the outer wall of the sample outlet of the dilution tube 130 is sleeved with a fourth sealing ring 152 and is in sealing connection with the second sample inlet of the amplification tube 120.
Further, the first sample inlet and the second sample inlet of the amplification tube 120 are respectively provided with a first diversion inclined plane 122 and a second diversion inclined plane 123, and the two diversion inclined planes are separated by a partition board; the bottoms of the two diversion slopes are provided with openings, so that the sample liquid can flow into the amplification tube 120 along the two diversion slopes after flowing out from the treatment tube 110 or the dilution tube 130; the bottom of the amplification tube 120 is sealed with a third sealing structure, which is preferably an aluminum film.
In some embodiments, referring to fig. 8, 13 and 14, the test cassette 140 is provided with at least one test strip 146, the test strip 146 extending along the height of the test device 100, and the test end of the test strip 146 being provided at the sample inlet of the test cassette 140.
Specifically, this scheme is a specific setting mode of test paper 146, and the detection box 140 is provided with at least one test paper 146, and the test paper 146 extends along the height direction of the detection device 100, and the detection end of the test paper 146 is disposed at the sample inlet of the detection box 140, so that the structure is compact, and the volume of the detection box 140 is reduced.
More specifically, one test paper 146 may be provided, or a plurality of test papers may be provided, so that multiple tests can be performed simultaneously, and the test results are compared with each other, so that accuracy is improved; preferably, two test strips 146 are provided, one on each side of the amplification tube 120.
Further, referring to fig. 14 and 15, according to some of the embodiments, the detection cartridge 140 has a cylindrical structure, the bottom of which has a bottom plate 141, and the bottom surface of the bottom plate 141 is provided with anti-slip patterns 1411; a driving piece 143 extending along the height direction is arranged at one side of the inside of the detection box 140, and the driving piece 143 is of a rod-shaped structure integrally formed on the detection box 140 and is used for jacking the first puncture structure 131; the middle position inside the detection box 140 is a detection area, the detection area is surrounded by the baffle 142 and is in butt joint with the sample outlet of the amplification tube 120, a third puncture structure extending along the height of the detection box 140 is integrally formed inside the detection area, the third puncture structure is preferably a cross structure, a larger opening can be formed on a third seal of the sample outlet of the amplification tube 120, and amplified samples can flow out conveniently; the inner walls of two sides of the detection area surrounded by the baffle 142 are respectively provided with a clamping groove 145 for fixing the detection ends of two test papers 146, the test papers 146 extend along the height of the detection device 100, the other ends penetrate through the outer structure 121 of the amplification tube 120 to reach the structure between the treatment tube 110 and the dilution tube 130, and the outer structure 121 of the amplification tube 120 and the connection structure 150 between the treatment tube 110 and the dilution tube 130 are respectively provided with a limiting structure 124 for fixing the test papers 146; in addition, the baffle 142 encloses a detection area, so that the sample to be detected flowing into the detection area and the diluent leaked from the driving part 143 can be prevented from being mixed and polluted.
In particular, the detection box 140 can be separately arranged with the amplification tube 120, or can be directly connected with the amplification tube 120 at the beginning of operation, and the third sealing structure is preferably an aluminum film with certain strength, so that the inner cavities of the detection box and the amplification tube cannot be communicated only by gravity, and the detection box is not influenced at the beginning of the process; after the diluent flows into the amplification tube 120 and the dilution is completed, the detection box 140 and the amplification tube 120 are forcibly inserted into each other, so that the inner cavities of the two are communicated.
Next, the present invention provides a detection method, to which the detection apparatus 100 of the first aspect is applied, including the steps of:
placing an initial sample into the processing tube 110 from a sample inlet of the processing tube 110, sealing the sample inlet of the processing tube 110, and processing the initial sample by a preset reagent in the processing tube 110;
after the initial sample is processed, the processing tube 110 is rotated, so that the first sealing structure is separated from the sample outlet of the processing tube 110, and the processing tube 110 is communicated with the inner cavity of the amplification tube 120;
the treated sample flows into the amplification tube 120, and a preset reagent in the amplification tube 120 amplifies the sample;
after the sample is amplified, the driving piece 143 on the detection box 140 drives the first puncture structure 131 to move upwards and puncture the second sealing structure, and the reagent preset in the dilution tube 130 flows into the amplification tube 120 and dilutes the amplified sample;
After the sample is diluted, the second puncture structure 144 of the detection box 140 punctures the third sealing structure of the sample outlet of the amplification tube 120, and the diluted sample liquid flows into the sample inlet of the detection box 140 and performs a chromatographic reaction through the test paper 146.
Specifically, the detection device 100 of the first aspect is applied to the scheme, and has the advantages of the detection device 100; in addition, the detection method is convenient to operate and is beneficial to improving the detection efficiency.
Preferably, the test strip 146 is a colloidal gold test strip 146. It should be noted that, the colloidal gold is gold particles formed by polymerizing chloroauric acid (HAuCl 4) under the action of reducing agents such as white phosphorus, ascorbic acid, sodium citrate, tannic acid and the like, and the gold particles are mutually repelled and suspended into a stable colloidal state due to the action of static electricity, so as to form a hydrophobic gel solution with negative electricity, and the name of the colloidal gold is derived from the gold particles. Colloidal gold has high electron density, can be combined with various biological macromolecules, and is a non-radioactive tracer which is more commonly used after fluorescein, radioisotope and enzyme are relayed by an immune labeling technology. In view of the negative charge presented on the surface of the colloidal gold, the colloidal gold can be combined with antigens and antibodies with positive charges on the surface through electrostatic action, and the combination does not damage the structure and the function of the protein. Therefore, colloidal gold test paper is generally used for antigen detection, and can detect the protein coat of viruses.
Further, in this embodiment, the primer required for amplification is bound to biotin in advance, and biotin can be bound to an antibody on the colloidal gold test strip 133, so that the colloidal gold test strip 133 can detect nucleic acid.
In particular, the sample may be blood, urine, a sample storage solution, or the like, or may be a nasopharyngeal swab from which the sample is collected.
In some embodiments, the step of "the treated sample flows into the amplification tube 120, and the preset reagent in the amplification tube 120 amplifies the sample" includes:
pressing the first portion 111 or the second portion 112 of the processing tube 110 causes the lumen of the processing tube 110 to contract, and the processed sample accelerates into the amplification tube 120.
Specifically, the method is to further optimize the amplification step of the processed sample, press the first portion 111 or the second portion 112 of the processing tube 110, so as to shrink the inner cavity of the processing tube 110, and accelerate the processed sample to flow into the amplification tube 120, thereby improving the flow speed of the sample and the detection efficiency.
Preferably, in some of these embodiments, pressing on the first portion 111 of the treatment tube 110 causes the treatment tube 110 lumen to contract. However, in other embodiments, it is within the scope of the present application to press on the second portion 112 of the processing tube 110 to constrict the lumen of the processing tube 110.
In summary, with reference to fig. 16, a detection flow will be described:
(1) Collecting a sample;
(2) Sample processing: the nucleic acid extraction reagent is placed in the treatment tube 110 in advance, an initial sample is placed from a sample inlet of the treatment tube 110, a cover 1111 is covered, and the nucleic acid extraction is performed by shaking;
(3) Nucleic acid amplification: after the sample is processed, the first part 111 of the processing tube 110 is rotated to drive the second part 112 to rotate, so that the sample outlet is separated from the first sealing structure, and the sample liquid after nucleic acid release flows into the amplification tube 120 from the processing tube 110 and flows into the amplification tube 120 through the first diversion inclined plane 122, and is uniformly shaken; enzymes, primers and the like required for the amplification reaction are pre-placed in the amplification tube 120; placing the amplification tube 120 in a heating device to perform constant-temperature heating for nucleic acid amplification reaction;
(4) Nucleic acid dilution: the diluent is pre-placed in the diluent tube 130; after the amplification reaction is finished, the amplification tube 120 is in butt joint with the detection box 140, so that the driving piece 143 of the detection box 140 lifts the first puncture structure 131 to puncture the second sealing structure of the dilution tube 130, and the dilution liquid flows out of the dilution tube 130 and flows into the amplification tube 120 through the second diversion inclined plane 123 for dilution;
(5) Chromatographic reaction: the amplification tube 120 is connected to the detection cartridge 140, and the second piercing structure 144 pierces the third sealing structure, so that the sample liquid flows into the detection cartridge 140 to perform a chromatographic reaction, thereby completing detection.
Preferably, the connection structure 150 or the external structure 121 is transparent, so as to facilitate observation of the detection result; the test strip 146 may be removed from the test device 100 for observation.
In particular, the detection device 100 and the method thereof according to the present embodiment are mainly used for nucleic acid detection, and in other embodiments, may be used for detecting other biochemical substances as long as the present embodiment can be implemented, and therefore the detection device 100 according to the present embodiment is not limited to nucleic acid detection only.
Although embodiments of the present invention have been described in connection with the accompanying drawings, various modifications and variations may be made by those skilled in the art without departing from the spirit and scope of the invention, and such modifications and variations fall within the scope of the invention as defined by the appended claims.
Claims (15)
1. A detection device, characterized in that the detection device (100) is for nucleic acid detection, comprising:
a processing tube (110);
an amplification tube (120) connected to the treatment tube (110);
a dilution tube (130) connected to the amplification tube (120);
a detection cartridge (140) connected to the amplification tube (120) or the dilution tube (130);
a first communication mechanism through which the processing tube (110) is communicable with the inner lumen of the amplification tube (120);
A second communication mechanism through which the amplification tube is communicable with the dilution tube (130) lumen;
a third communication mechanism through which the dilution tube (130) is communicable with the inner cavity of the cartridge (140); or, the third communication mechanism is arranged at the assembly position of the amplification tube (120) and the detection box (140), and the amplification tube (120) can be communicated with the inner cavity of the detection box (140) through the third communication mechanism.
2. The device according to claim 1, wherein the first communication mechanism is a rotational communication mechanism, and wherein the processing tube (110) and the amplification tube (120) rotate relative to each other to communicate the lumens.
3. The test device of claim 2, wherein the first communication mechanism comprises a first seal structure;
the first sealing structure is used for sealing a sample outlet of the processing tube (110), the sample outlet of the processing tube (110) is eccentrically arranged relative to the axis of the processing tube (110), the processing tube (110) and the amplifying tube (120) enable the sample outlet of the processing tube (110) to be separated from the first sealing structure through relative rotation, and enable the processing tube (110) to be communicated with an inner cavity of the amplifying tube (120);
Or, the first sealing structure is used for sealing the sample inlet of the amplification tube (120), the sample inlet of the amplification tube (120) is eccentrically arranged relative to the axis of the amplification tube (120), the treatment tube (110) and the amplification tube (120) enable the sample inlet of the amplification tube (120) to be separated from the first sealing structure through relative rotation, and enable the treatment tube (110) to be communicated with the inner cavity of the amplification tube (120).
4. A device according to claim 3, wherein the lumen of the processing tube (110) is arranged to be collapsible such that the sample liquid in the processing tube (110) is accelerated to flow into the amplification tube (120).
5. The detection device according to claim 4, characterized in that the treatment tube (110) comprises a first portion (111) and a second portion (112), the first portion (111) being connected to the second portion (112) in an axially movable, circumferentially limited manner.
6. The detection device according to any one of claims 1 to 5, wherein the sample inlet of the amplification tube (120) comprises a first sample inlet and a second sample inlet;
the first sample inlet communicates with the sample outlet of the processing tube (110) through the first communication mechanism, and the second sample inlet communicates with the sample outlet of the dilution tube (130) through the second communication mechanism;
The sample outlet of the amplification tube (120) communicates with the sample inlet of the cartridge (140) through the third communication mechanism.
7. The device according to claim 6, wherein the second communication means comprises a second sealing structure and a first piercing structure (131) adapted to pierce the second sealing structure, the second sealing structure being arranged on one of the second sample inlet and the sample outlet of the dilution tube (130), the first piercing structure (131) being arranged on the other.
8. The device according to claim 7, wherein the second sealing structure is provided at a sample outlet of the dilution tube (130), the first piercing structure (131) is provided at the second sample inlet, and the second communicating mechanism further comprises a driving member (143), and the driving member (143) is configured to drive the first piercing structure (131) to move upward and pierce the second sealing structure.
9. The device according to claim 8, wherein the driving member (143) is arranged in the cartridge (140), and wherein the driving member (143) is arranged coaxially with the first piercing structure (131).
10. The device according to claim 9, wherein the third communication means comprises a third sealing structure and a second piercing structure (144) adapted to pierce the third sealing structure, the third sealing structure being arranged on one of the sample outlet of the amplification tube (120) and the sample inlet of the cartridge (140), the second piercing structure (144) being arranged on the other.
11. The device according to claim 10, wherein the third sealing structure is provided at a sample outlet of the amplification tube (120), the second piercing structure (144) is provided at a sample inlet of the cartridge (140), and a height of the second piercing structure (144) is lower than a height of the driving member (143).
12. The detection apparatus according to claim 6, wherein the treatment tube (110) and the dilution tube (130) are above the amplification tube (120), and the treatment tube (110) and the dilution tube (130) are juxtaposed.
13. The device according to claim 12, wherein the cartridge (140) is provided with at least one test strip (146), the test strip (146) extending in a height direction of the device (100), and a detection end of the test strip (146) being provided at a sample inlet of the cartridge (140).
14. A detection method, characterized in that a detection device (100) according to any one of claims 1 to 13 is applied, comprising the steps of:
placing an initial sample into a processing tube (110) from a sample inlet of the processing tube (110), sealing the sample inlet of the processing tube (110), and processing the initial sample by a preset reagent in the processing tube (110);
After the initial sample is processed, the processing tube (110) is rotated, so that the first sealing structure is separated from the sample outlet of the processing tube (110), and the processing tube (110) is communicated with the inner cavity of the amplification tube (120);
the treated sample flows into the amplification tube (120), and a preset reagent in the amplification tube (120) amplifies the sample;
after the sample is amplified, a driving piece (143) on the detection box (140) drives the first puncture structure (131) to move upwards and puncture the second sealing structure, and a reagent preset in the dilution tube (130) flows into the amplification tube (120) and dilutes the amplified sample;
after the sample is diluted, the second puncture structure (144) of the detection box (140) punctures the third sealing structure of the sample outlet of the amplification tube (120), and diluted sample liquid flows into the sample inlet of the detection box (140) and performs chromatographic reaction through test paper (146).
15. The method according to claim 14, wherein the step of flowing the treated sample into the amplification tube (120), and the step of amplifying the sample with a predetermined reagent in the amplification tube (120) comprises:
pressing the first portion (111) or the second portion (112) of the processing tube (110) to shrink the inner cavity of the processing tube (110), and accelerating the processed sample to flow into the amplification tube (120).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311426910.1A CN117535130A (en) | 2023-10-30 | 2023-10-30 | Detection device and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311426910.1A CN117535130A (en) | 2023-10-30 | 2023-10-30 | Detection device and method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117535130A true CN117535130A (en) | 2024-02-09 |
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ID=89785218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311426910.1A Pending CN117535130A (en) | 2023-10-30 | 2023-10-30 | Detection device and method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117535130A (en) |
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2023
- 2023-10-30 CN CN202311426910.1A patent/CN117535130A/en active Pending
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