CN117512053A - Inspection reagent for detecting helicobacter pylori spheroids and spirochetes under gastroscope and application thereof - Google Patents
Inspection reagent for detecting helicobacter pylori spheroids and spirochetes under gastroscope and application thereof Download PDFInfo
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- CN117512053A CN117512053A CN202311716464.8A CN202311716464A CN117512053A CN 117512053 A CN117512053 A CN 117512053A CN 202311716464 A CN202311716464 A CN 202311716464A CN 117512053 A CN117512053 A CN 117512053A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
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- G01N2333/908—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
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Abstract
The invention provides an inspection reagent for detecting helicobacter pylori (helicobacter pylori) spheroids and spirochetes under a gastroscope and application thereof, belonging to the technical field of biomedicine. An inspection reagent for detecting helicobacter pylori spheroids, which takes anthocyanin as a pH value indicator and also comprises hydrogen peroxide. The pH value is raised by utilizing the hydrogen peroxide which is a substrate with the activity of the catalase of the helicobacter pylori spheroid, and the distribution condition of the helicobacter pylori spheroid on gastric mucosa is shown according to the change of anthocyanin color. The method for detecting based on the detection reagent belongs to a noninvasive helicobacter pylori detection method, can directly detect whether the helicobacter pylori spheroid exists under a gastroscope, detects whether the spheroid residue exists after the helicobacter pylori resistance treatment, and has extremely high clinical popularization and application values.
Description
Technical Field
The invention belongs to the technical field of biomedicine, and particularly relates to an inspection reagent for detecting helicobacter pylori spirochete and spheroids under a gastroscope and application thereof.
Background
Helicobacter pylori (Helicobacter pylori) is an important pathogenic bacterium in the stomach, which can cause duodenal ulcers, chronic active gastritis, chronic atrophic gastritis, gastric mucosa tissue-related lymphoma, and gastric cancer. Therefore, it is necessary to simply and conveniently diagnose the presence or absence of helicobacter pylori infection and to distinguish the relationship between helicobacter pylori infection and intragastric diseases.
The state of viability of helicobacter pylori under normal conditions is the shape of the helicobacter pylori, leading to helicobacter pylori related diseases. Under the action of various adverse factors in vitro and in vivo, the morphology of helicobacter pylori changes from a spiroid to a spheroid. The helicobacter pylori spheroids still have vitality and strong resistance to the external adverse environment, and the conditions allow the spheroids to recover to the shape of the helicobacter body. It has been confirmed by studies that helicobacter pylori spheroids colonize and reproduce in the stomach of mice and cause gastritis. Helicobacter pylori spheroids are important factors that lead to the spread of helicobacter pylori among the population and to the failure of helicobacter pylori treatment to re-burn.
The existing methods for detecting helicobacter pylori include morphological detection, functional detection and immunological detection. Helicobacter pylori morphological detection includes HE staining, W-S silver staining, giemsa staining and methylene blue staining of gastric mucosal biopsies to reveal helicobacter pylori morphology, and bacterial culture. A functional detection method of helicobacter pylori utilizes the urease activity of helicobacter pylori to decompose urea into ammonia (NH) 3 ) And carbon dioxide (CO) 2 ) Helicobacter pylori, such as breath tests and rapid urease detection methods. Helicobacter pylori immunoassay the presence of helicobacter pylori infection is assessed by detecting helicobacter pylori antibodies in blood and feces.
Means for performing these detection methods include invasive and non-invasive inspection methods. The non-invasive helicobacter pylori detection method comprises expiration experiment, blood antibody detection and fecal antibody detection, and the invasive detection method comprises the steps of performing gastroscopy to obtain biopsy tissue of gastric mucosa under a gastroscope for helicobacter pylori detection, including rapid urease detection, tissue section staining detection and bacteria culture.
The detection of helicobacter pylori by the expiration test has the advantage of high sensitivity, but has the defects that the expiration test can only judge whether helicobacter pylori infection exists or not, and the condition of occurrence in the stomach caused by helicobacter pylori is uncertain; the helicobacter pylori antibody detection method can only determine whether helicobacter pylori is infected or not, and cannot determine whether helicobacter pylori infection exists in the current body. Rapid urease detection, tissue section staining detection and bacterial culture have the advantage of good specificity, but have the disadvantage that biopsy tissue of gastric mucosa needs to be taken out to detect the presence or absence of helicobacter pylori during examination, because helicobacter pylori is distributed in a focus in the stomach, false negative and missed diagnosis are caused if no helicobacter pylori infection site is taken by biopsy.
The existing technology for detecting helicobacter pylori by utilizing the biological enzyme activity of helicobacter pylori comprises a rapid urease method and an expiration experiment method, wherein the helicobacter pylori has the urease activity to decompose urea into ammonia (NH) 3 ) And carbon dioxide (CO) 2 ) The principle of (2) detecting helicobacter pylori, this mechanism can detect only the morphology of helicobacter pylori, and currently there is no test reagent for the spheroids of helicobacter pylori.
Disclosure of Invention
In view of the above, an object of the present invention is to provide a reagent for directly detecting helicobacter pylori spheroids under a gastroscope, wherein the helicobacter pylori spheroids have catalase activity, the pH value around helicobacter pylori is raised by the action of substrate hydrogen peroxide, and the purpose of detecting helicobacter pylori spheroids on gastric mucosa is achieved according to the color change of a pH value indicator.
The invention provides an inspection reagent for detecting helicobacter pylori spheroids, which comprises a pH value indicator anthocyanin or anthocyanin derivative and hydrogen peroxide.
Preferably, the mass ratio of the hydrogen peroxide to the anthocyanin is (0.5-1.5): (0.5-1.5).
Preferably, water is also included;
the working concentration of the hydrogen peroxide is 3.33% -10%;
the working concentration of the anthocyanin is 3.33% -10%.
The invention provides a test reagent for simultaneously detecting helicobacter pylori spheroids and spirochetes, which comprises the test reagent and substrate urea for detecting spirochetes.
Preferably, the mass ratio of urea to hydrogen peroxide to anthocyanin is (1-3): (0.5-1.5): (0.5-1.5).
Preferably, the working concentration of the urea is 6.67-20%.
Preferably, the anthocyanin has a structure shown in formula I:
wherein R is 1 And R is 2 is-H, -OH or-OCH 3 ；R 3 is-H or glycosyl; r is R 4 is-OH or glycosyl.
The invention provides application of the test reagent in preparing a product for detecting helicobacter pylori spheroids.
The invention provides application of the test reagent in preparing a product for evaluating helicobacter pylori treatment effect.
The invention provides application of the test reagent in preparing a product for screening gastric diseases related to helicobacter pylori infection;
the stomach-related disease includes at least one of: duodenal ulcers, chronic active gastritis, chronic atrophic gastritis, gastric mucosa tissue-associated lymphoma, and gastric cancer.
The invention provides an inspection reagent for detecting helicobacter pylori spheroids, which takes anthocyanin as a pH value indicator and also comprises substrate hydrogen peroxide. The helicobacter pylori spheroids have catalase activity, and the catalase can decompose hydrogen peroxide into water (H) 2 O) and oxygen (O) 2 ). The hydrogen peroxide is weak acid, and after the hydrogen peroxide is decomposed into water and oxygen, the local pH value is changed into neutral from weak acid, so that the distribution of helicobacter pylori spheroids can be displayed according to the change of the pH value indicator color. Therefore, the detection reagent provided by the invention can accurately judge the spherical body of helicobacter pyloriThe detection has the characteristics of good specificity and high sensitivity, is low in cost and simple and convenient to operate, provides great convenience for detecting different forms of helicobacter pylori, and is suitable for clinical popularization and application.
The invention also provides a test reagent for simultaneously detecting the helicobacter pylori spheroid and the spirochete, which is based on the helicobacter pylori spheroid having the catalase activity and the helicobacter pylori spirochete having the urease activity, and can decompose urea into ammonia (NH) 3 ) And carbon dioxide (CO) 2 ) The pH value of the living area of the helicobacter pylori is increased, and after urea substrate is added, the existence and distribution of the helicobacter pylori spheroid and the helicobacter pylori can be simultaneously displayed due to the color change of the existence of the pH value indicator. Respectively using
The presence and distribution of helicobacter pylori spheroids and spirochetes can be shown respectively by using anthocyanin as a pH indicator and hydrogen peroxide as a substrate and using anthocyanin as a pH indicator and urea as a substrate. Therefore, the detection reagent provided by the invention not only can accurately detect the helicobacter pylori which is being infected, but also can accurately judge different forms (spirochetes and spheroids) of the helicobacter pylori, and the detection reagent provided by the invention has the characteristics of good specificity and high sensitivity, is low in cost and simple and convenient to operate, and provides great convenience for detecting different forms of the helicobacter pylori, thereby being suitable for clinical popularization and application.
Drawings
FIG. 1 shows the gastroscopic results of a helicobacter pylori negative patient;
FIG. 2 shows the results of gastroscopy before spraying the test reagent on patients with helicobacter pylori infected chronic gastritis;
FIG. 3 shows the gastroscopic results of a patient suffering from helicobacter pylori infected chronic gastritis after spraying the test agent;
FIG. 4 shows the gastroscopic results of a patient suffering from helicobacter pylori spheroid infection;
FIG. 5 shows the gastroscopic examination results of a patient with gastric mucosa-associated tissue lymphoma;
FIG. 6 shows the result of gastroscopy before the gastric cancer patient sprays the detection reagent;
FIG. 7 shows the result of gastroscopy after gastric cancer patients spray detection reagent on gastric mucosa.
Detailed Description
The invention provides an inspection reagent for detecting helicobacter pylori spheroids under a gastroscope, which comprises a pH indicator anthocyanin or anthocyanin derivative and a substrate hydrogen peroxide.
In the present invention, the hydrogen peroxide is a catalytic substrate for catalase produced by helicobacter pylori spheroids, and the hydrogen peroxide is decomposed into water (H 2 O) and oxygen (O) 2 ) The hydrogen peroxide is weak acid, the partial pH value is changed into neutral from weak acid after being decomposed, and the pH value indicator indicates the color change to show the distribution of the helicobacter pylori spheroids. The mass ratio of the hydrogen peroxide to the anthocyanin is preferably (0.5-1.5): (0.5 to 1.5), more preferably 1:1. The detection reagent, when in use, preferably also comprises water. The working concentration of hydrogen peroxide is preferably 3.33% to 10%, more preferably 6.67%. The working concentration of the anthocyanin is preferably 3.33% -10%, more preferably 6.67%.
In the present invention, the anthocyanin is used as a pH indicator. The anthocyanin has a structure shown in a formula I:
wherein R is 1 And R is 2 is-H, -OH or-OCH 3 ；R 3 is-H or glycosyl; r is R 4 is-OH or glycosyl. The anthocyanin molecules have high molecular conjugated systems, contain acidic and basic groups, and are easy to dissolve in polar solvents such as water, methanol, ethanol, dilute alkali, dilute acid and the like. The ultraviolet and visible light regions have strong absorption, the maximum absorption wavelength of the ultraviolet region is near 280nm, and the maximum absorption wavelength of the visible light region is in the range of 500-550 nm. The color of anthocyanin substance changes along with the change of pH value, the pH is less than 7 and is red, and the anthocyanin substance is purple when the pH is 7-8, and the pH is less than 7>And at 11, blue. Repeated experiments prove that the anthocyanin substance is the pH indicator which is most suitable for displaying helicobacter pylori under an endoscope. Because of the gastric mucosa itselfThe normal color of the composition is red, gastric acid is acidic, anthocyanin is red in an acidic environment, the pH value is raised by an action substrate under the condition that helicobacter pylori exists, the color change of the anthocyanin serving as a pH indicator can be clearly compared with the red color of gastric mucosa, and the distribution condition of helicobacter pylori in the stomach can be clearly shown. This is also one of the features of the present invention. The anthocyanin preferably comprises an anthocyanin isolated from: purple cabbage, purple sweet potato, grape, blood orange, red cabbage, blueberry, eggplant, cherry, red strawberry, mulberry, hawthorn and morning glory. In an embodiment of the present invention, the anthocyanin preferably comprises a purple cabbage pigment. Purple cabbage pigments were purchased from Qingdao Pengyuan Kang Hua Natural products Co.Ltd.
The invention also provides an inspection reagent for simultaneously detecting the helicobacter pylori spheroids, which comprises a pH value indicator anthocyanin or anthocyanin derivative, a substrate hydrogen peroxide and urea.
In the invention, the urea is a catalytic substrate of urease generated by helicobacter pylori helicoidal body, and is decomposed into ammonia (NH) under the action of the urease 3 ) And carbon dioxide (CO) 2 ) The pH value of the living area of the helicobacter pylori is raised, and the color change is generated in the presence of the anthocyanin serving as a pH value indicator, so that the distribution of the helicobacter pylori is displayed. The mass ratio of the urea to the hydrogen peroxide to the anthocyanin is (1-3): (0.5-1.5): (0.5 to 1.5), more preferably 2:1:1. The detection reagent, when in use, preferably also comprises water. The working concentration of urea is preferably 6.67% to 20%, more preferably 13.33%.
In the present invention, when the test agent is used for gastroscopy, the agent is sprayed onto the gastric mucosal surface using a spray tube passing through a gastroscopy forceps channel. The spraying dosage of the detection reagent is 30-60 ml/time.
The invention provides application of the detection reagent for detecting helicobacter pylori spirochete and spheroid in preparing products for detecting helicobacter pylori spirochete and/or helicobacter pylori spheroid.
In the present invention, the product preferably comprises a kit.
In the present invention, the method for detecting helicobacter pylori spheroids preferably comprises the steps of:
mixing hydrogen peroxide, anthocyanin and water to prepare working concentration to obtain a helicobacter pylori spheroid detection reagent;
spraying the helicobacter pylori spheroid detection reagent on the surface of the gastric mucosa to be detected, which is the same in source, wherein when the color of the gastric mucosa surface reagent is unchanged, the gastric mucosa is free from infection of the helicobacter pylori spheroid; when the color of the gastric mucosa surface reagent changes from light red to blue, the infection of helicobacter pylori spheroids on the gastric mucosa is indicated.
In the present invention, the method for detecting helicobacter pylori spheroids and spirochetes preferably comprises the steps of:
urea, hydrogen peroxide, anthocyanin and water are mixed to prepare working concentration, and helicobacter pylori spirochete and/or spheroid detection reagent working solution is obtained;
spraying the detection reagent working solution on the surface of the gastric mucosa to be detected, observing the color change of the surface of the gastric mucosa, and when the color of the reagent on the surface of the gastric mucosa is red and has no change, indicating that the gastric mucosa has no infection of helicobacter pylori spirochete and/or helicobacter pylori spheroid; when the color of the gastric mucosa surface reagent changes from red to blue, the infection of helicobacter pylori on the gastric mucosa is indicated;
to further determine whether the infected helicobacter pylori is in the form of a helix or a spheroid, detection reagents were further formulated:
urea, anthocyanin and water are mixed to prepare working concentration, and helicobacter pylori spirochete detection reagent is obtained;
spraying the helicobacter pylori spirochete testing agent on the surface of the gastric mucosa to be tested, which is the same as the source, and when the color of the gastric mucosa surface agent is not changed, indicating that the gastric mucosa is not infected by helicobacter pylori spirochete; when the color of the gastric mucosa surface reagent changes from red to blue, the infection of helicobacter pylori spirochete on the gastric mucosa is indicated;
mixing hydrogen peroxide, anthocyanin and water to prepare working concentration to obtain a helicobacter pylori spheroid detection reagent;
spraying the helicobacter pylori spheroid detection reagent on the surface of the gastric mucosa to be detected, which is the same in source, wherein when the color of the gastric mucosa surface reagent is unchanged, the gastric mucosa is free from infection of the helicobacter pylori spheroid; when the color of the gastric mucosa surface reagent changes from light red to blue, the infection of helicobacter pylori spheroids on the gastric mucosa is indicated.
In the invention, the detection reagent can detect whether helicobacter pylori is infected or not in real time by using a gastroscope for observation, and can also carry out other relevant detection on the gastric mucosa biopsy tissue of the color-changing part, thereby having great operation convenience, safety and no side effect.
Since helicobacter pylori spheroids are important factors affecting the transmission of helicobacter pylori in the population and are also important factors which are not radically cured and re-combusted after helicobacter pylori treatment, the invention provides application of the detection reagent for simultaneously detecting helicobacter pylori spheroids and spheroids in preparing products for evaluating the helicobacter pylori treatment effect and/or the helicobacter pylori transmissibility.
In the invention, the helicobacter pylori treatment effect is evaluated by adopting the detection method, and the helicobacter pylori detection reagent working solution is sprayed on the gastric mucosa surface to be detected, so that a negative detection result (the color of the gastric mucosa surface reagent is not changed in red) appears, and the helicobacter pylori spheroid detection reagent is sprayed on the gastric mucosa of the same source again, so that when the positive detection result (the color of the gastric mucosa surface reagent is changed from red to blue-purple) appears, the helicobacter pylori of the gastric mucosa of a patient to be detected is shown to have spherical change, and the helicobacter pylori is not eradicated, so that the possibility of afterburning is realized.
Since helicobacter pylori is an important pathogenic bacterium of the stomach, various stomach diseases can be caused to occur and develop, the invention provides the application of the detection reagent for simultaneously detecting helicobacter pylori spirochete and spheroid in preparing products for screening stomach diseases related to helicobacter pylori infection.
In the present invention, the gastric disease associated with helicobacter pylori infection preferably includes at least one of the following: duodenal ulcers, chronic active gastritis, chronic atrophic gastritis, gastric mucosa tissue-associated lymphoma, and gastric cancer. In the embodiment of the invention, the infection state of helicobacter pylori in various stomach diseases is clarified by screening the inducement of the gastric diseases related to helicobacter pylori infection, thereby providing a direction for the subsequent treatment of the stomach diseases.
The following is a detailed description of a test reagent for detecting helicobacter pylori spirochetes and spheroids under gastroscope and its application, provided by the present invention, but it should not be construed as limiting the scope of the present invention.
Example 1
Inspection reagent for simultaneously detecting helicobacter pylori spirochete and spheroid and use method thereof
1. Reagent composition: solid urea, solid hydrogen peroxide and solid purple cabbage pigment;
2. preparation of the reagent: the solid urea, the solid hydrogen peroxide and the solid purple cabbage pigment are respectively weighed and mixed with 4g, 2g and 2g, and dissolved in 30ml of water to obtain the detection reagent working solution.
3. Method for using detection reagent
During gastroscopy, sucking the gastric fluid of a patient through a gastroscopy forceps pipeline, sucking 30ml of detection reagent working fluid into a syringe, inserting a spray pipe into the gastroscopy forceps pipeline, spraying the detection reagent working fluid onto the surface of a gastric mucosa, and observing the change condition of the reagent color on the surface of the gastric mucosa through a gastroscope:
helicobacter pylori spirochete has urease activity and can decompose urea into ammonia (NH) 3 ) And carbon dioxide (CO) 2 ) The pH value of the living area of helicobacter pylori is increased. Helicobacter pylori spheroids do not produce urease but produce catalase, which can decompose hydrogen peroxide into water (H 2 O) and oxygen (O) 2 )，2H 2 O 2 ＝2H 2 O+O 2 . Hydrogen peroxide is weakly acidic, and after the hydrogen peroxide is decomposed into water and oxygen, the local pH value is changed from weakly acidic to neutral. Purple cabbage pigment is used as pH value indicator and is red in acidity, blue in neutrality,The alkalinity is green, and the color change of the purple cabbage pigment as a pH value indicator shows the helicobacter pylori distribution.
After spraying the detection reagent, the color of the gastric mucosa surface reagent is red and consistent with the color of gastric mucosa and does not change. After spraying the detection reagent on the person with helicobacter pylori spirochete or spheroid infection, the color of the gastric mucosa surface reagent changes from red to blue.
In order to determine the form of helicobacter pylori, helicobacter pylori detection reagents (solid urea 4g, solid purple cabbage pigment 2g, dissolved in 30ml water) and helicobacter pylori spheroid detection reagents (solid hydrogen peroxide 2g, solid purple cabbage pigment 2g, dissolved in 30ml water) are respectively prepared, gastric mucosa is sprayed again to detect, and according to the results of the detection reagents, whether helicobacter pylori infected by a patient is helicobacter pylori or helicobacter pylori spheroid can be judged.
Example 2
Experiment for detecting different stomach illness patients by adopting detection reagent
The relationship between helicobacter pylori infection and chronic gastritis, gastric cancer and gastric mucosa tissue-related lymphoma was examined, and the detection of helicobacter pylori and spheroids was carried out according to the detection reagent of example 1 while gastroscopy was carried out on chronic gastritis patients, gastric cancer patients and gastric lymphoma patients, and the detection procedure was the same as in example 1.
FIG. 1 shows the result of gastroscopy of a patient negative for helicobacter pylori, and as can be seen from FIG. 1, the gastric mucosa surface is smooth and flat, and no color change occurs after spraying the detection reagent, which indicates that the patient has neither helicobacter pylori infection nor helicobacter pylori spheroid infection.
FIG. 2 shows the result of gastroscopy before spraying the test agent on patients with helicobacter pylori infected chronic gastritis, and it can be seen from FIG. 2 that the gastric mucosa surface is rough and scattered on the erosion focus. FIG. 3 shows the result of gastroscopy after spraying the test agent, and as can be seen from FIG. 3, the partial area of the gastric mucosa surface is clearly observed to show a flaky bluish purple color change, which indicates that the patient is currently infected with helicobacter pylori.
FIG. 4 shows the result of gastroscopy of a patient suffering from helicobacter pylori spheroid infection, who has been treated with a drug and who has been exhaled in an experiment to detect helicobacter pylori negative for gastroscopy. As can be seen from FIG. 4, the gastric mucosa of the patient is swollen, the color of the patient is unchanged after the gastric mucosa is sprayed with the helicobacter pylori spiral physical examination test agent, and the gastric mucosa shows light blue after the gastric mucosa is sprayed with the helicobacter pylori spheroid detection agent, which indicates that the patient is infected with the helicobacter pylori spheroid at present, and the possibility of reburning exists.
Fig. 5 shows the result of gastroscopy of a patient with gastric mucosa-associated tissue lymphoma, and as can be seen from fig. 5, the gastric mucosa swelling mucosa folds of the patient disappear, but the mucosa is intact. After the detection reagent is sprayed, the color change of blue-violet can be obviously observed on gastric mucosa, which indicates that the patient has positive helicobacter pylori.
FIG. 6 shows the result of gastroscopy before spraying the gastric cancer patient with the detection reagent, and it can be seen from FIG. 6 that there is an obvious ulcer-type tumor in the shape of volcanic mouth at the lesser curvature of the antrum, and the mucosa surrounding the tumor and the central ulcer. After spraying the detection reagent, the periphery of the tumor is light blue, and in fig. 7, a linear color change of blue-violet can be obviously observed on the gastric mucosa of the lesser curvature of the stomach body, which indicates that the gastric cancer patient has helicobacter pylori infection.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. An inspection reagent for detecting helicobacter pylori spheroids, which is characterized by comprising anthocyanin or anthocyanin derivatives as a pH value indicator and hydrogen peroxide.
2. The test agent for detecting a spherical body of helicobacter pylori according to claim 1, characterized in that the mass ratio of hydrogen peroxide to anthocyanin is (0.5 to 1.5): (0.5-1.5).
3. The test reagent of claim 1 or 2, further comprising water;
the working concentration of the hydrogen peroxide is 3.33% -10%;
the working concentration of the anthocyanin is 3.33% -10%.
4. A test reagent for simultaneous detection of helicobacter pylori spheroids and spirochetes, characterized by comprising the test reagent according to claim 1 or 2 and a substrate urea for detection of spirochetes.
5. The test kit according to claim 4, wherein the mass ratio of urea, hydrogen peroxide and anthocyanin is (1 to 3): (0.5-1.5): (0.5-1.5).
6. The test reagent according to claim 4 or 5, wherein the urea has a working concentration of 6.67% to 20%.
7. The test agent of any one of claims 1, 2, 4 and 5, wherein the anthocyanin has the structure of formula I:
wherein R is 1 And R is 2 is-H, -OH or-OCH 3 ；R 3 is-H or glycosyl; r is R 4 is-OH or glycosyl.
8. Use of the test agent according to any one of claims 1 to 7 for the preparation of a product for detecting helicobacter pylori spheroids.
9. Use of the test agent according to any one of claims 1 to 7 for the preparation of a product for evaluating the therapeutic effect of helicobacter pylori.
10. Use of the test agent according to any one of claims 1 to 7 for the preparation of a product for screening gastric diseases associated with helicobacter pylori infection;
the stomach-related disease includes at least one of: duodenal ulcers, chronic active gastritis, chronic atrophic gastritis, gastric mucosa tissue-associated lymphoma, and gastric cancer.
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| CN202311716464.8A CN117512053A (en) | 2023-12-13 | 2023-12-13 | Inspection reagent for detecting helicobacter pylori spheroids and spirochetes under gastroscope and application thereof |
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| CN202311716464.8A CN117512053A (en) | 2023-12-13 | 2023-12-13 | Inspection reagent for detecting helicobacter pylori spheroids and spirochetes under gastroscope and application thereof |
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