CN117503991A - Cellulose collagen wound hydrogel dressing and preparation method thereof - Google Patents
Cellulose collagen wound hydrogel dressing and preparation method thereof Download PDFInfo
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- CN117503991A CN117503991A CN202311548349.4A CN202311548349A CN117503991A CN 117503991 A CN117503991 A CN 117503991A CN 202311548349 A CN202311548349 A CN 202311548349A CN 117503991 A CN117503991 A CN 117503991A
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 58
- 108010035532 Collagen Proteins 0.000 title claims abstract description 58
- 229920001436 collagen Polymers 0.000 title claims abstract description 58
- 239000000017 hydrogel Substances 0.000 title claims abstract description 53
- 239000001913 cellulose Substances 0.000 title claims abstract description 43
- 229920002678 cellulose Polymers 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- -1 schiff base compounds Chemical class 0.000 claims abstract description 13
- 239000002262 Schiff base Substances 0.000 claims abstract description 9
- 239000008367 deionised water Substances 0.000 claims description 16
- 229910021641 deionized water Inorganic materials 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 16
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 claims description 10
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- NGDIAZZSCVVCEW-UHFFFAOYSA-M sodium;butyl sulfate Chemical compound [Na+].CCCCOS([O-])(=O)=O NGDIAZZSCVVCEW-UHFFFAOYSA-M 0.000 claims description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 5
- 229960003638 dopamine Drugs 0.000 claims description 5
- 239000003242 anti bacterial agent Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- YFMPSMITLLBENU-UHFFFAOYSA-N 3,4-dihydroxybenzylamine Chemical compound NCC1=CC=C(O)C(O)=C1 YFMPSMITLLBENU-UHFFFAOYSA-N 0.000 claims description 3
- JDMNLLVJYLMTPE-UHFFFAOYSA-N 4-(3-aminopropyl)benzene-1,2-diol Chemical compound NCCCC1=CC=C(O)C(O)=C1 JDMNLLVJYLMTPE-UHFFFAOYSA-N 0.000 claims description 3
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims description 3
- 239000004599 antimicrobial Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Substances CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 claims description 2
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 claims 2
- 208000027418 Wounds and injury Diseases 0.000 abstract description 20
- 206010052428 Wound Diseases 0.000 abstract description 19
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 15
- 230000029663 wound healing Effects 0.000 abstract description 6
- 230000000052 comparative effect Effects 0.000 description 9
- 150000001299 aldehydes Chemical class 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 230000006835 compression Effects 0.000 description 4
- 238000007906 compression Methods 0.000 description 4
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 125000001841 imino group Chemical group [H]N=* 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011056 performance test Methods 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000218378 Magnolia Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 150000004753 Schiff bases Chemical class 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
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- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- 238000007037 hydroformylation reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
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- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004043 responsiveness Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000000518 rheometry Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- 210000001519 tissue Anatomy 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0052—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a cellulose collagen wound hydrogel dressing and a preparation method thereof, and belongs to the technical field of dressings. The cellulose collagen wound hydrogel dressing comprises the following components in parts by weight: modified collagen: 2-5 parts; aldehyde-modified cellulose: 2-3 parts; schiff base compounds: 0.3-0.5 part; water: 98-100 parts. The cellulose collagen hydrogel dressing with the double-network structure has excellent antibacterial capability and mechanical property, can promote wound healing of patients, and is simple in preparation method and convenient to operate.
Description
Technical Field
The invention belongs to the technical field of dressing, and particularly relates to a cellulose collagen wound hydrogel dressing and a preparation method thereof.
Background
The hydrogel dressing has a three-dimensional network composed of hydrophilic substances, is insoluble in water, and has a water absorption capacity which can be up to 1000 times of the self weight; meanwhile, the wound healing device is transparent, and the wound healing condition of a patient can be monitored at any time under the condition that the dressing is not removed in actual operation; the gel can not adhere to the wound and the healthy skin around the wound, and can not cause secondary injury to the wound when removed. The natural hydrogel has better biodegradability, and meanwhile, the natural hydrogel has good biocompatibility due to the characteristics similar to extracellular matrix, is safer and more environment-friendly, and is more and more concerned due to the good biocompatibility and degradability.
The natural high molecular hydrogel has the advantages of good biocompatibility, cell responsiveness and the like, but the biomechanical property and degradation performance of the natural high molecular hydrogel have certain limitations in tissue engineering application, and the application of the hydrogel is often severely limited by the mechanical properties of the natural high molecular hydrogel. Collagen is a biodegradable non-antigenic polymer, plays roles in stopping bleeding and promoting cell adhesion and proliferation in the healing process, and hydrogel taking collagen as a main building unit is widely applied to the field of biological medicine and the field of wearable equipment due to excellent biocompatibility and abundant active groups on side chains. As a wound dressing material, antibacterial property is a key factor, and a dressing having antibacterial property can protect a wound from bacterial infection, but collagen hydrogel has defects of low hardness, poor mechanical properties, poor antibacterial property, poor shape stability, poor drug release effect, poor plasticity and the like, and thus, some defects and disadvantages existing in the collagen hydrogel are to be solved.
Disclosure of Invention
The invention aims to provide a cellulose collagen wound hydrogel dressing and a preparation method thereof, which are used for solving the problem of poor antibacterial capability of collagen hydrogel.
The aim of the invention can be achieved by the following technical scheme:
the cellulose collagen wound hydrogel dressing comprises the following raw materials in parts by weight:
modified collagen: 2-5 parts;
aldehyde-modified cellulose: 2-3 parts;
schiff base compounds: 0.3-0.5 part;
water: 98-100 parts;
wherein the modified collagen is prepared by the following steps:
adding collagen into deionized water, stirring at 50 ℃, adding an antibacterial agent and Tris-HCl buffer solution, stirring uniformly, adjusting pH to 4-5 by hydrochloric acid solution, and drying to constant weight to obtain the modified collagen.
Preferably, the Schiff base compound is one of cystamine dihydrochloride, N-hydroxysuccinimide and 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride.
Preferably, the antibacterial agent is one of dopamine, 3, 4-dihydroxybenzyl amine hydrobromide and 4- (3-aminopropyl) benzene-1, 2-diol.
Preferably, the ratio of collagen, deionized water, antimicrobial agent and Tris-HCl buffer is 2g:50mL:0.5g:5mL; wherein the pH of the Tris-HCl buffer is 6.8; the concentration of the hydrochloric acid solution is 1mol/L.
Preferably, the hydroformylation cellulose is prepared by the steps of:
adding cellulose and sodium periodate into deionized water, stirring in a dark condition, adding ethylene glycol, stirring, washing with deionized water, and vacuum drying at 40 ℃ to obtain the aldehyde cellulose.
Preferably, the dosage ratio of cellulose, sodium periodate, deionized water and ethylene glycol is 2g:1.5g:100mL:3g.
Preferably, the preparation method of the cellulose collagen wound hydrogel dressing comprises the following steps:
adding the modified collagen and the aldehyde cellulose into deionized water, stirring at 50 ℃, adjusting the pH value to 4-5 by using a sodium hydroxide solution, adding a Schiff base compound, stirring, pouring into a polytetrafluoroethylene mould, and standing for molding to obtain the cellulose collagen wound hydrogel dressing.
The invention has the beneficial effects that:
according to the invention, the antibacterial agent is grafted on the collagen, so that the collagen generates a special antibacterial mechanism due to the conjugation effect of electrons on a phenolic hydroxyl structure, and the collagen has weak binding capacity with hydrogen ions and is easy to dissociate, so that the active hydrogen ions can neutralize active oxygen such as free radicals and the like to achieve the effect of eliminating the free radicals, and the oxidation resistance of the hydrogel is further enhanced; and the phenolic hydroxyl can also react with proteins in cell membranes, so that bacteria are denatured and can be inhibited from growing on the surface of the dressing, the antibacterial performance of the wound dressing is enhanced and improved, and inflammation is reduced to promote wound healing.
According to the invention, after hydroxyl groups on the surface of cellulose are oxidized to generate aldehyde groups with stronger chemical reaction, the mechanical property of the hydrogel can be improved, and modified collagen with good antibacterial effect and aldehyde cellulose generate reversible imino groups after being reacted by Schiff base, so that on one hand, the modified collagen and the aldehyde cellulose are crosslinked to form hydrogel with antibacterial effect, on the other hand, the generation of imino groups improves the crosslinking density, the elasticity of the prepared hydrogel is enhanced, the rebound resilience and fatigue resistance of the hydrogel can be further improved, and the hydrogel can be subjected to larger deformation.
The wound dressing prepared by the invention has good antibacterial, antioxidant and anti-inflammatory properties and good rebound resilience performance, has a self-healing function, can be used for promoting wound healing aiming at the effects generated in multiple stages of wound healing, is an ideal material for wounds, and has the advantages of simple steps, few procedures and convenient popularization.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The present example provides an aldehyde-modified cellulose:
adding 2g of cellulose and 1.5g of sodium periodate into 100mL of deionized water, stirring to be uniform under the dark condition, adding 3g of ethylene glycol, stirring for 1h, washing with deionized water for 3 times, washing for 40min each time, and drying in a vacuum drying oven at 40 ℃ for 24h to obtain the aldehyde cellulose.
Example 2
The present example provides a modified collagen:
2g of collagen (CAS number 9064-67-9, shanghai Michelia Biochemical technology Co., ltd.) was added to 50mL of deionized water, stirred to uniformity in a constant temperature water bath at 50 ℃, 0.5g of dopamine and 5mLTris-HCl buffer (pH 6.8) were added, stirred to uniformity, pH was adjusted to 4.5 using 1mol/L hydrochloric acid solution, and dried to constant weight, to obtain modified collagen.
Example 3
The dopamine in example 2 was replaced with 3, 4-dihydroxybenzyl amine hydrobromide, and the other raw materials and steps were the same as in example 2.
Example 4
The dopamine of example 2 was replaced with 4- (3-aminopropyl) benzene-1, 2-diol, and the remaining materials and steps were the same as in example 2.
Example 5
The present example provides a cellulosic collagen wound hydrogel dressing:
adding 3 parts of modified collagen in example 2 and 2 parts of aldehyde cellulose into 100 parts of deionized water, stirring for 40min to be uniform in a water bath kettle at 50 ℃, adjusting the pH to 4.5 by a sodium hydroxide solution (the concentration is 1 mol/L), adding 0.35 part of cystamine dihydrochloride, stirring uniformly, pouring into a polytetrafluoroethylene mould, and standing for molding to obtain the cellulose collagen wound hydrogel dressing.
Example 6
The modified collagen in example 5 was replaced with the modified collagen in example 3, cystamine dihydrochloride was replaced with N-hydroxysuccinimide, and the other raw materials and steps were the same as in example 5.
Example 7
The modified collagen in example 5 was replaced with the modified collagen in example 4, and the other raw materials and steps were the same as those in example 5.
Comparative example 1
The modified collagen in example 5 was replaced with collagen, and the other raw materials and steps were the same as in example 5.
Comparative example 2
The cystamine dihydrochloride in example 5 was replaced with glutaraldehyde, and the other raw materials and steps were the same as in example 5.
Comparative example 3
The modified collagen in example 5 was replaced with collagen, the cellulose aldehyde was replaced with cellulose, the cystamine dihydrochloride was replaced with glutaraldehyde, and the other raw materials and steps were the same as in example 5.
Performance tests were performed on examples 5-7 and comparative examples 1-3:
mechanical property test: preparing the hydrogel into a long strip shape at 25 ℃, and testing the tensile strength and the elongation at break of the hydrogel by adopting an electronic universal tester with the model of WDW-02; the compression performance of the hydrogel cylinder with the diameter of 60mm and the height of 10mm is tested by an electronic universal tester, the compression speed is 5mm/min, the test is stopped immediately after the compression, the average value is obtained by three parallel tests, and the compression performance of the hydrogel is tested.
Swelling property test: the hydrogels were freeze-dried and each sample was about 100mg (noted as W d ) Immersed in a beaker containing 100mL of phosphate buffer solution at room temperature. Taking out the sample at a certain time interval, sucking the water on the surface of the hydrogel by the filter paper, weighing, and taking the weight as W s Expressed, according to the formula 100% × (W s -W d )/W d The swelling ratio was calculated.
Antibacterial activity test: antibacterial activity of hydrogels against staphylococcus aureus (s.aureus, ATCC 25923) and escherichia coli (e.coli, ATCC 22922) was examined by the zone of inhibition method. The bacterial culture medium consisted of yeast powder (0.5%, w/v), peptone (1.0%, w/v), naCl (1.0%, w/v) and agar powder (1.5%, w/v). The hydrogel was placed on a filter paper having a diameter of 5mm, the medium was placed in an incubator at 37.5℃and after 48 hours the experimental phenomenon was recorded and the diameter of the zone of inhibition was measured.
Elastic performance test: the rheology of the hydrogel was measured with a TA rheometer, the rheometer was preheated to 37 ℃ before the start of the experiment, 350 μl of the hydrogel pre-polymerization solution was poured into the bottom plate of the rheometer, the oscillation mode-time scan was performed on the hydrogel pre-polymerization solution, and the storage modulus of the hydrogel was measured after 10 min.
The test results are shown in table 1:
TABLE 1
As can be seen from Table 1, the mechanical properties, elastic properties, swelling ratio and antibacterial property effects in examples 5 to 7 were excellent. Compared with the example 5, the comparative example 1 adopts unmodified collagen, the comparative example 2 adopts a cross-linking agent glutaraldehyde to replace schiff base compounds, the antibacterial effect of the hydrogel in the comparative example 1 is poor according to the data in the table, the mechanical property of the hydrogel in the comparative example 2 is poor, and the combination of the comparative examples 1-3 shows that the cellulose collagen wound hydrogel dressing prepared by the invention has excellent antibacterial property, elastic property and mechanical property.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The cellulose collagen wound hydrogel dressing is characterized by comprising the following raw materials in parts by weight:
modified collagen: 2-5 parts;
aldehyde-modified cellulose: 2-3 parts;
schiff base compounds: 0.3-0.5 part;
water: 98-100 parts;
wherein the modified collagen is prepared by the following steps:
adding collagen into deionized water, stirring at 50 ℃, adding an antibacterial agent and Tris-HCl buffer solution, stirring uniformly, adjusting pH to 4-5 by hydrochloric acid solution, and drying to constant weight to obtain the modified collagen.
2. The cellulose collagen wound hydrogel dressing according to claim 1, wherein the schiff base compound is one of cystamine dihydrochloride, N-hydroxysuccinimide and 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride.
3. The cellulose collagen wound hydrogel dressing according to claim 1, wherein the antimicrobial agent is one of dopamine, 3, 4-dihydroxybenzyl amine hydrobromide and 4- (3-aminopropyl) benzene-1, 2-diol.
4. The cellulose collagen wound hydrogel dressing according to claim 1, wherein the ratio of collagen, deionized water, antimicrobial agent and Tris-HCl buffer is 2g:50mL:0.5g:5mL; wherein the pH of the Tri s-HCl buffer is 6.8; the concentration of the hydrochloric acid solution was 1mol/L.
5. The cellulose collagen wound hydrogel dressing according to claim 1, wherein the aldehyde-modified cellulose is prepared by the steps of:
adding cellulose and sodium periodate into deionized water, stirring in a dark condition, adding ethylene glycol, stirring, washing with deionized water, and vacuum drying at 40 ℃ to obtain the aldehyde cellulose.
6. The cellulose collagen wound hydrogel dressing according to claim 5, wherein the ratio of the amounts of cellulose, sodium periodate, deionized water and ethylene glycol is 2g:1.5g:100mL:3g.
7. The cellulose collagen wound hydrogel dressing according to claim 1, wherein the method of preparing the cellulose collagen wound hydrogel dressing comprises the steps of:
adding the modified collagen and the aldehyde cellulose into deionized water, stirring at 50 ℃, adjusting the pH value to 4-5 by using a sodium hydroxide solution, adding a Schiff base compound, stirring, pouring into a polytetrafluoroethylene mould, and standing for molding to obtain the cellulose collagen wound hydrogel dressing.
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| CN117860641A (en) * | 2024-03-12 | 2024-04-12 | 山东赫柏芳香生物科技有限公司 | Production process of moisturizing skin-care emulsion cosmetics |
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| CN117860641A (en) * | 2024-03-12 | 2024-04-12 | 山东赫柏芳香生物科技有限公司 | Production process of moisturizing skin-care emulsion cosmetics |
| CN117860641B (en) * | 2024-03-12 | 2024-05-17 | 山东赫柏芳香生物科技有限公司 | Production process of moisturizing skin-care emulsion cosmetics |
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