CN117491667A

CN117491667A - Chemiluminescent analyzer

Info

Publication number: CN117491667A
Application number: CN202311569119.6A
Authority: CN
Inventors: 蔡传良; 何胜宽; 钟奕贤; 许遵浩; 杜超; 肖文君; 刘文林; 田锋; 周兆海; 张金德; 雷丽娟; 刘琪; 左月霞; 刘理顺; 刘紫薇
Original assignee: Shenzhen Tailede Medical Co ltd
Current assignee: Shenzhen Tailede Medical Co ltd
Priority date: 2023-11-23
Filing date: 2023-11-23
Publication date: 2024-02-02

Abstract

The invention relates to the technical field of chemiluminescent immunoassay, in particular to a chemiluminescent analyzer. The reaction cup rotary table of the chemiluminescent analyzer drives the reaction cup to move when rotating, and in the reaction cup moving process, the reaction cup can pass through the reading area of the reading module and the shaking accommodating space on the shaking piece. The shaking piece is driven by the shaking driving mechanism, so that the shaking piece can shake the reaction cup, and the reagent in the reaction cup is uniformly mixed. After the reagent is evenly mixed, the reaction cup turntable can rotate to enable the reaction cup to move to a reading area of the reading module, and the reading module reads the luminous value of the reagent in the reaction cup. The ingenious reaction cup lower part that makes exposes in this application to shake the reaction cup through shaking piece and reaction cup lower part contact, the mixing of reagent in the reaction cup and the process of reading the value are all accomplished in the module of mixing the reading the value. Through the rotatory switching process of reaction cup carousel, process switching efficiency is higher, has improved the technical problem that current full automation luminescence tester detection efficiency is low.

Description

Chemiluminescent analyzer

Technical Field

The invention relates to the technical field of chemiluminescent immunoassay, in particular to a chemiluminescent analyzer.

Background

Most of the current full-automatic chemiluminescence analyzers include a manipulator or chain reaction tube transfer mechanism, a sample needle, and a reagent needle. During detection, firstly, the serum of a sample to be detected is added into a reaction tube through a sample needle, then a reagent needle extracts a reaction reagent and is added into the reaction tube for reaction, then a mechanical arm or a chain conveyor belt is required to move the reaction tube into a reaction module for reaction, after the reaction is completed, the reaction tube is required to be continuously transferred to a cleaning module for cleaning and adsorbing magnetic beads, and finally the detection is carried out.

For example, chinese patent publication No. CN218412570U discloses a full-automatic chemical luminescence analyzer, which includes a waste cartridge module, a reagent processing module, a scanning module, a sample processing module, a cleaning liquid and waste liquid tank, a cleaning device module, a reaction module, a mixing module, a reading module, a TIP head module, an incubation module, a three-dimensional arm module, and a pipetting device. The three-dimensional arm pipetting device replaces a mechanical arm or a grabbing, transferring, taking and placing reaction cup, and the TIP head is matched to replace a sample adding needle to add samples and reagents, so that cross contamination is avoided. However, in such a full-automatic luminescence analyzer, there are many steps in which the cuvette needs to be picked up by a pipetting device for replacement, and there is a technical problem of low detection efficiency.

Disclosure of Invention

The invention provides a chemiluminescent analyzer, which is used for solving the technical problem of low detection efficiency of the traditional fully-automatic chemiluminescent analyzer.

According to a first aspect, in one embodiment there is provided a chemiluminescent assay device comprising a rack, a sample processing module, an incubation module, a cleaning device module, and a blending read module; the mixing reading module is used for mixing the reagents uniformly and detecting the luminescence value of the reaction; the blending read value module comprises:

the reading module is used for reading the luminous value of the reagent in the reaction cup;

the reaction cup rotating disc comprises a suspension part, wherein the suspension part is provided with a reaction cup hole, and the reaction cup hole is used for accommodating the reaction cup and enabling the lower end of the reaction cup to extend out;

the rotary table driving mechanism is used for driving the rotary table of the reaction cup to rotate so as to drive the reaction cup to move to a reading area of the reading module;

the shaking piece is positioned below the suspended part and is provided with a shaking accommodating space which is positioned on a movement path of the reaction cup turntable for driving the reaction cup to move so that the reaction cup can enter the shaking accommodating space;

And the shaking driving mechanism is used for driving the shaking piece to move so that the shaking piece acts on the part of the reaction cup in the shaking accommodating space to shake the reaction cup after the reaction cup enters the shaking accommodating space.

Further, in an embodiment, the shaking member is rotatably disposed on the frame, the shaking driving mechanism drives the shaking member to rotate, and the shaking member acts on the reaction cup to shake the reaction cup in the rotation process.

In a further embodiment, the rotation axis of the shaking-up piece and the central line of the reaction cup hole are arranged at intervals in parallel.

Further, in one embodiment, the shaking-up accommodating space has a first opening and a second opening; when the reaction cup moves to the shaking accommodating space along with the reaction cup turntable, one of the first opening and the second opening is used for the reaction cup to enter the shaking accommodating space, and the other one is used for the reaction cup to leave the shaking accommodating space.

Still further, in one embodiment, the shaking-up member has a shaking-up groove, the space in the shaking-up groove forming the shaking-up accommodation space, the shaking-up groove having a groove bottom wall, a first groove side wall, and a second groove side wall, the shaking-up groove having a top opening opposite the groove bottom wall, the first opening and the second opening each communicating with the top opening; the shaking-up piece shakes the reaction cup through the side wall of the first groove and/or the side wall of the second groove.

Further, in an embodiment, the blending value reading module includes a base configured on the frame and a light shield covering the upper side of the base, the light shield and the base enclose a darkroom, and the reaction cup turntable, the value reading module and the shaking piece are all located in the darkroom; the shaking driving mechanism is arranged at the lower side of the base, and the shaking piece is arranged at the upper side of the base.

Further, in one embodiment, the chemiluminescent analyzer comprises a substrate refrigeration assembly comprising a refrigeration case and a refrigeration module for refrigerating the refrigeration case, wherein the refrigeration case is provided with a refrigeration case side opening, the refrigeration case side opening is used for entering and exiting the refrigeration case, and a refrigeration case frame is arranged at the top of the refrigeration case and is used for hanging a substrate bottle;

the refrigerator box side opening is in the front side of refrigerator box, be equipped with on the left side lateral wall of refrigerator box and be used for supporting the left side backup pad of refrigerator box frame left end, be equipped with on the right side lateral wall of refrigerator box and be used for supporting the right side backup pad of refrigerator box frame right end, left side backup pad is decurrent downwards gradually from left to right, right side backup pad is decurrent downwards gradually from right to left.

In a further embodiment, the side opening of the refrigeration box is positioned at the front side of the refrigeration box, the side wall of the rear side of the refrigeration box is provided with a limit groove for the substrate bottle to enter, and the limit groove is used for limiting the substrate bottle to swing left and right.

Further, in one embodiment, the chemiluminescent analyzer comprises a housing, a material guide penetrating the housing, and a waste bin fixed outside the housing; the guide piece stretches into one end in the shell is provided with a waste receiving port, one end stretching out of the shell is provided with a waste outlet, and the waste box is positioned at the lower side of the waste outlet and is used for receiving waste falling from the guide piece.

Further, in one embodiment, the sample processing module includes a sample tube rack module, a sample tube rack box, and a tube rack driving mechanism, the sample tube rack module includes a tube rack seat and a sample tube rack rotatably disposed on the tube rack seat, the tube rack driving mechanism drives the sample tube rack to rotate, and the sample tube rack has a sample tube accommodating space for accommodating a sample tube; sample pipe support module cartridge is in the sample pipe support box, pipe support actuating mechanism disposes on the sample pipe support box, pipe support actuating mechanism includes driving motor and drive gear train, be fixed with driven gear on the sample pipe support, drive gear train includes drive gear, drive gear and intermediate gear, drive gear with sample pipe support module one-to-one, in the same sample pipe support module: driven gears on adjacent sample tube frames are meshed; the transmission gear is meshed with at least one driven gear in the corresponding sample pipe rack module; the driving gear is meshed with at least one intermediate gear, and the intermediate gear is meshed with the adjacent transmission gears simultaneously, so that the driving gear drives each transmission gear to rotate.

According to the chemiluminescent determinator of the embodiment, the chemiluminescent determinator comprises a blending reading module, the blending reading module comprises a reaction cup turntable, the reaction cup turntable drives the reaction cup to move when rotating, and in the movement process of the reaction cup, the reaction cup can pass through a reading area of the reading module and a shaking accommodating space on a shaking piece. The shaking piece is driven by the shaking driving mechanism, so that the shaking piece can shake the reaction cup, and the reagent in the reaction cup is uniformly mixed. After the reagent is evenly mixed, the reaction cup turntable can rotate to enable the reaction cup to move to a reading area of the reading module, and the reading module reads the luminous value of the reagent in the reaction cup. Compared with the prior chemiluminescent determinator, the liquid transfer device is adopted to pick up the reaction cup to switch the position of the reaction cup, the lower part of the reaction cup is skillfully exposed in the application, the reaction cup is rocked by rocking the member to be contacted with the lower part of the reaction cup, and the mixing and the value reading processes of reagents in the reaction cup are all completed in the mixing and value reading module. Through the rotatory switching process of reaction cup carousel, process switching efficiency is higher, has improved the technical problem that current full automation luminescence tester detection efficiency is low.

Drawings

FIG. 1 is a schematic diagram of a chemiluminescent assay according to one embodiment;

FIG. 2 is a flow chart of test operations in one embodiment;

FIG. 3 is a schematic structural view of a reaction cup according to an embodiment;

FIG. 4 is a diagram of the internal architecture of a mix read module in one embodiment;

FIG. 5 is a schematic diagram of a structure of another view angle of the blending read module in one embodiment;

FIG. 6 is a schematic diagram of a mask of a blending read module in an embodiment;

FIG. 7 is a top view of the lower portion of the cuvette entering the shake-up accommodation space in one embodiment;

FIG. 8 is a schematic diagram of a sample processing module in one embodiment;

FIG. 9 is a schematic diagram of a sample tube rack module according to one embodiment;

FIG. 10 is a schematic view of a partial structure of a sample tube rack module according to an embodiment;

FIG. 11 is a schematic diagram of a substrate refrigeration assembly according to one embodiment;

FIG. 12 is an exploded view of a substrate refrigeration assembly in one embodiment;

FIG. 13 is a schematic view showing the structure of a cooling box in one embodiment;

FIG. 14 is a schematic view of the structure of a substrate bottle and a refrigerated cassette rack in one embodiment;

FIG. 15 is a schematic view of the mounting structure of the leader and the reject box in one embodiment;

FIG. 16 is a schematic view of an embodiment of a lid panel after installation.

List of feature names corresponding to reference numerals in the figure: 1. a frame; 2. a sample processing module; 3. an incubation module; 4. a cleaning device module; 5. a blending reading module; 6. a reaction cup; 61. a large diameter section; 62. a small diameter section; 7. a waste cartridge module; 8. a three-dimensional arm module; 9. a substrate refrigeration assembly; 10. a pipetting device; 11. a reaction module; 12. cleaning liquid and waste liquid barrels; 13. a scanning module; 14. a reagent processing module; 15. a TIP head module;

21. A sample tube rack module; 211. a tube stand seat; 215. a sample tube spring; 2111. a holding hole; 212. a sample tube rack; 2121. a sample tube receiving groove; 213. a driven gear; 214. a bearing seat; 2141. a pipe rack mounting groove; 2142. bearing pedestal boss; 215. a sample tube spring; 22. a sample tube rack box; 231. a driving motor; 232. a drive gear; 233. a transmission gear; 2331. a first transmission gear; 2332. a second transmission gear; 2333. a third transmission gear; 234. an intermediate gear; 2341. a first intermediate gear; 2342. a second intermediate gear; 24. a sensor; 25. a motor base; 2501. perforating a motor shaft;

51. a reading module; 5101. a mounting plate; 5102. a photomultiplier tube; 52. a reaction cup turntable; 521. a suspended portion; 522. a reaction cup hole; 531. a turntable motor; 532. a rotary table driving belt wheel; 533. a turntable driven belt wheel; 534. a turntable synchronous belt; 535. the first limiting optocoupler; 54. shaking up the piece; 541. shaking up the accommodating space; 542. a first opening; 543. a second opening; 544. a bottom wall of the tank; 545. a first slot sidewall; 546. a second groove sidewall; 547. a trigger handle; 551. shaking up the motor; 552. shaking up the driving wheel; 553. shaking up the driven wheel; 554. shaking up the synchronous belt; 56. mixing and reading a value base; 57. a light shield; 571. a reaction cup taking and placing hole; 58. a mouth covering mechanism; 581. a rotary driving mechanism; 582. a baffle; 59. a support column; 510. a light shield support column; 511. a light shielding gasket; 512. the second limiting optocoupler; 513. a mask support column; 514. a light shielding plate; 5141. the reaction cup avoids the hole;

71. A housing; 72. a waste bin; 73. a waste cartridge carrier; 74. a waste cartridge cover plate; 75. a material guide; 751. a waste receiving port; 752. a waste outlet;

91. a refrigeration box; 911. a refrigeration box side opening; 912. a left side support plate; 913. a right side support plate; 914. a limit groove; 92. a refrigeration module; 920. the heat preservation sponge; 921. the heat preservation foam is arranged below; 922. left heat preservation foam; 923. the right side of the heat preservation foam; 924. after heat preservation and foam soaking; 93. a refrigeration box rack; 94. a substrate bottle; 95. a substrate bottle cover plate; 96. a temperature sensor; 97. a fuse; 98. a seal ring; 99. an elbow; 9101. an inclined surface; 9102. and a drain hole.

Description of bracketed reference numerals in the drawings: in the bracketed reference numerals in the drawings, features indicated by the reference numerals are features indicated by both numerals in the brackets and numerals outside the brackets.

Detailed Description

The invention will be described in further detail below with reference to the drawings by means of specific embodiments. Wherein like elements in different embodiments are numbered alike in association. In the following embodiments, numerous specific details are set forth in order to provide a better understanding of the present application. However, one skilled in the art will readily recognize that some of the features may be omitted, or replaced by other elements, materials, or methods in different situations. In some instances, some operations associated with the present application have not been shown or described in the specification to avoid obscuring the core portions of the present application, and may not be necessary for a person skilled in the art to describe in detail the relevant operations based on the description herein and the general knowledge of one skilled in the art.

Furthermore, the described features, operations, or characteristics of the description may be combined in any suitable manner in various embodiments. Also, various steps or acts in the method descriptions may be interchanged or modified in a manner apparent to those of ordinary skill in the art. Thus, the various orders in the description and drawings are for clarity of description of only certain embodiments, and are not meant to be required orders unless otherwise indicated.

The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The terms "coupled" and "connected," as used herein, are intended to encompass both direct and indirect coupling (coupling), unless otherwise indicated.

In one embodiment, referring to fig. 1 to 16, the chemiluminescent analyzer comprises a rack 1, a sample processing module 2, an incubation module 3, a cleaning device module 4, and a mixing reading module 5. The sample processing module 2 is used for containing a sample tube and processing samples in the sample tube, the incubation module 3 is used for placing a reaction cup and providing an incubation environment for the reaction cup, the cleaning device module 4 is used for cleaning magnetic beads, and the mixing reading module 5 is used for mixing substrate liquid uniformly and detecting a luminescence value of a reaction.

Referring to fig. 1 to 6, in order to improve the measurement efficiency of the reagent, the mixing module and the reading module of the reagent are integrated. Limited by the installation space, the reaction cup 6 is uniformly shaken, so that reagents in the reaction cup 6 are uniformly mixed. The blending read module 5 will be described in detail below.

The mixing read value module 5 comprises a read value module 51 for reading the luminous value of the reagent in the reaction cup 6, a reaction cup rotary table 52, a rotary table driving mechanism for driving the reaction cup rotary table 52 to rotate, a shaking piece 54 and a shaking driving mechanism.

To facilitate the installation of the shaking-up member 54, the cuvette turntable 52 herein includes a suspending portion 521, and the suspending portion 521 has a cuvette hole 522 therein, where the cuvette hole 522 allows the cuvette 6 to be inserted therein and the lower end of the cuvette 6 to protrude. The shaking element 54 is below the suspended portion 521. After the reaction cup 6 extends downwards out of the reaction cup hole 522, the shaking piece 54 is convenient to apply an acting force to the reaction cup 6, so that the reaction cup 6 shakes, and reagents in the reaction cup 6 are uniformly mixed.

The size of the cuvette hole 522 should be sufficient to shake the cuvette 6. Referring to fig. 3, the reaction cup 6 includes a large diameter section 61 with a larger outer diameter and a small diameter section 62 with a smaller outer diameter, wherein the cup opening is located on the large diameter section 61. After the cuvette 6 is inserted into the cuvette aperture 522, the large diameter section 61 is positioned above the cuvette turntable 52, and the small diameter section 62 is inserted into the cuvette aperture 522 and extends out of the cuvette aperture 522. The outer diameter of the small diameter section 62 should be smaller than the diameter of the reaction cup hole 522, and a gap for allowing the reaction cup 6 to shake is reserved between the outer wall of the small diameter section 62 and the wall of the reaction cup hole 522.

The turntable driving mechanism drives the reaction cup turntable 52 to rotate, and the reaction cup 6 can pass through the reading area of the reading module 51, namely the reading port of the reading module 51, and can also shake by the shaking piece 54 through the position of the shaking piece 54 in the rotating process. The reaction cup 6 passes through the shaking piece 54, so that the reagent is fully mixed and reacted, and then moves to the reading area of the reading module 51, and the reading module 51 reads the luminous value of the reagent in the reaction cup 6.

In order to implement the shaking motion of the shaking member 54 on the reaction cup 6, in this application, the shaking member 54 has a shaking accommodating space 541, and the shaking accommodating space 541 is located on a motion path of the reaction cup turntable 52 to drive the reaction cup 6 to move, so that the reaction cup 6 can enter the shaking accommodating space 541.

The shaking driving mechanism is used for driving the shaking member 54 to move, so that the shaking member 54 acts on the part of the reaction cup 6 in the shaking accommodating space 541 to shake the reaction cup 6.

The shaking piece 54 is driven to move through the shaking driving mechanism, after the lower portion of the reaction cup 6 moves to the shaking accommodating space 541, the shaking piece 54 can act on the lower portion of the reaction cup 6, the reaction cup 6 is shaken, and reagents in the reaction cup 6 are uniformly mixed. After the reagents in the reaction cup 6 are uniformly mixed, the reaction cup turntable 52 can drive the reaction cup 6 to a reading area, and the luminous value of the reagents is read through the reading module 51. In the process of uniformly mixing and reading the values, a pipetting device is not required to pick up the reaction cup 6 to change the position of the reaction cup 6, so that the process switching efficiency of the reaction cup 6 is improved, and the detection efficiency of the chemiluminescent analyzer is further improved.

In one embodiment, referring to FIGS. 4 and 5, the number of cuvette holes 522 in the cuvette carousel 52 is at least two. Specifically, two, three or more than four may be employed. Of course, in some other embodiments, the number of cuvette holes 522 in the cuvette carousel 52 may be one.

Under the condition that the number of the reaction cup holes 522 is at least two, each reaction cup hole 522 is uniformly arranged on the reaction cup turntable 52, the reaction cup turntable 52 rotates one hole position each time, when the reaction cup 6 in one reaction cup hole 522 is in a reading area, the reaction cup 6 in the other reaction cup hole 522 is in the reaction cup hole 522, and thus when the reading module 51 reads a value of one reaction cup 6, the shaking piece 54 can shake the other reaction cup 6 to shake the same, and the testing efficiency is further improved. Of course, to further improve the test efficiency, the number of the read value modules 51 may be increased, for example, there may be two read value modules 51, and there may be two corresponding shaking members 54, so that the test efficiency may be doubled.

In one embodiment, the shaking-up and turntable drive mechanisms may take any feasible form, for example, the drive mechanism may include a drive motor and gears through which shaking member 54 is driven to rotate; the reaction cup turntable 52 can also be driven to rotate by a driving motor and a gear; for another example, the cuvette carousel 52 may be directly rotated by a stepper motor; for another example, the shaking element 54 may be in a reciprocating manner, and the shaking driving mechanism may be a driving motor or a cylinder.

Further, in an embodiment, referring to fig. 4 and 5, the shaking element 54 is rotatably disposed on the frame 1, and the shaking driving mechanism drives the shaking element 54 to rotate. Still further, in one embodiment, the axis of rotation of the shaking element 54 is spaced parallel to the centerline of the cuvette aperture 522. In this way, the reaction cup hole 522 and the shaking element 54 are always in an eccentric state, and the reaction cup 6 is easier to shake when the shaking element 54 rotates.

In order to facilitate applying a force to the reaction cup 6, in one embodiment, referring to fig. 4 and 5, the shaking-up accommodating space 541 has a first opening 542 and a second opening 543, one of the first opening 542 and the second opening 543 is configured to allow the reaction cup 6 to enter the shaking-up accommodating space 541, and the other is configured to allow the reaction cup 6 to leave the shaking-up accommodating space 541.

Specifically, in one embodiment, the shaking-up member 54 has a shaking-up groove, the space in the shaking-up groove forms a shaking-up accommodation space 541, the shaking-up groove has a groove bottom wall 544, a first groove side wall, and a second groove side wall, the shaking-up groove has a top opening opposite to the groove bottom wall 544, and the first opening 542 and the second opening 543 are all communicated with the top opening. The shaking-up member 54 shakes the cuvette 6 through the first tank sidewall 545 and/or the second tank sidewall 546. The shaking-up groove is convenient for driving the reaction cup 6 to act.

In an embodiment, the first opening 542 is a reaction cup inlet, the second opening 543 is a reaction cup outlet, when the shaking member is at the initial position, the first opening 542 and the second opening 543 are both located on the movement path of the reaction cup, when the reaction cup turntable rotates, the reaction cup can be driven to enter the shaking accommodating space 541 through the first opening 542, after the mixing is completed, the reaction cup turntable continues to rotate, and the reaction cup is driven to pass through the second opening 543 and leave the shaking accommodating space 541 to move towards the reading area of the reading module 51.

Specifically, referring to fig. 7, in one embodiment, referring to fig. 4, the shaking-up member 54 is an eccentric, and the shaking-up groove is located on the eccentric. The contact position of the first groove side wall 545 and the lower part of the reaction cup 6 is in an eccentric state, and in the rotation process of the shaking piece 54, the first groove side wall 545 can reciprocally push the lower part of the reaction cup 6 to drive the lower part of the reaction cup 6 to shake rapidly. Referring to the figure, the first groove sidewall 545 and the second groove sidewall 546 are both arc-shaped, the first groove sidewall 545 is concave arc-shaped, and the second groove sidewall is convex arc-shaped, so that the reaction cup 6 is convenient to enter the shaking accommodating space 541.

In order to coordinate the relationship between the shaking-up part 54 and the reaction cup turntable 52, the lower part of the reaction cup 6 smoothly enters the shaking-up accommodating space 52, the mixing read value module 5 comprises a second limiting optocoupler 512, and when the reagent in the reaction cup 6 is mixed, the shaking-up driving mechanism controls the stopping of the shaking-up part 54 according to the detection result of the second limiting optocoupler 512, so that the shaking-up part 54 can return to the initial reset, and the shaking-up stopping and starting positions of each time are guaranteed to be at the positions, so that the reaction cup 6 can smoothly enter and exit the shaking-up accommodating space 52 when the turntable rotates.

In some other embodiments, the shaking element may be a cam, and the cam pushes the lower portion of the reaction cup 6 to shake the reaction cup 6.

In one embodiment, referring to fig. 4 to 6, the blending read module 5 includes a blending read base 56 disposed on the rack 1 and a light shield 57 covering an upper side of the blending read base 56, the light shield 57 and the blending read base 56 enclose a darkroom, the light shield 57 has a reaction cup taking hole 571 for the reaction cup 6 to enter and exit the darkroom, the blending read module 5 includes a shielding mechanism 58 in the darkroom, and the shielding mechanism 58 is used for opening the reaction cup taking hole 571 when the reaction cup 6 is taken in and out, and covering the reaction cup taking hole 571 when the blending read is operated. In one embodiment, the second limiting optocoupler 512 is disposed on the mix readout base 56. The second spacing optocoupler 512 may employ a laser sensor. Correspondingly, the shaking-up piece 54 has a trigger handle 547 adapted to the second limiting optocoupler 512, when the trigger handle 547 rotates to the trigger area of the second limiting optocoupler 512, the second limiting optocoupler 512 is triggered, and can send a position signal as a controller, and the controller controls the shaking-up piece driving mechanism according to the signal.

The shaking driving mechanism comprises a shaking motor 551, and the shaking motor 551 can drive the shaking piece 54 to work in various modes, for example, the shaking motor 551 can drive the shaking piece 54 to reciprocate forward and backward for multiple times, so that the shaking effect is better; for another example, the shaking-up motor 551 may drive the shaking-up member 54 to continuously rotate in the forward direction or the reverse direction for shaking-up; for another example, the shaking-up motor 551 may intermittently drive the shaking-up member 54 to move. Regardless of the working mode, the initial position of the shaking element 54 is a trigger area where the trigger handle 547 triggers the second limiting optocoupler 512, that is, a position where the second limiting optocoupler 512 is blocked, the shaking motor 551 is controlled in a closed loop, and can control the stop position of the shaking element 54 according to the signal of the second limiting optocoupler 512, and after the shaking element 54 finishes shaking the reaction cup 6 each time, the shaking element 54 returns to the initial position.

In some other embodiments, the second limiting optocoupler 512 may also employ a mechanical sensor for detecting the rotational position of the shaking member.

In one embodiment, referring to fig. 4 and 5, the cuvette carousel 52, the reading module 51, and the shaking unit 54 are all in a darkroom. The shaking driving mechanism is arranged at the lower side of the mixing read value base 56, and the shaking member 54 is arranged at the upper side of the mixing read value base 56. The shaking driving mechanism and the shaking member 54 are arranged on the upper and lower sides of the mixing read value base 56, and by arranging the shaking driving mechanism outside the darkroom, the space of the darkroom is not occupied.

Specifically, in one embodiment, referring to fig. 4 to 6, the blending read module 5 includes a support column 59 disposed on a bottom plate, and the blending read base 56 is fixed on the support column 59. A mask support column 510 is fixed on the blending read base 56, and the mask 57 is covered on the mask support column 510.

A shading washer 511 is arranged between the shading cover 57 and the blending reading base 56, and after the shading cover 57 is fixed on the shading cover support column 510 through a fastener, the shading washer 511 is pressed.

The shutter 58 includes a rotary drive mechanism 581 provided on one side of the cuvette turntable 52, and a shutter 582 fixed to the rotary drive mechanism 581, the shutter 582 having a blocking position for blocking the cuvette access hole 571 and a release position for releasing the cuvette access hole 571. The rotation driving mechanism 581 drives the shutter 582 to rotate, and the position of the shutter 582 is switched. The rotary driving mechanism 581 may employ a rotary electromagnet, a driving motor, or the like.

The read module 51 includes a mounting plate 5101 secured to a mix read base 56 and photomultiplier tube 5102 on one side of the cuvette carousel 52. The photomultiplier tube 5102 is fixed to the mounting plate 5101, and the mounting plate 5101 is fixed to the mix readout base 56.

4 cuvette holes 522 are uniformly distributed on the cuvette turntable 52. The turntable drive mechanism includes a turntable motor 531 secured to the mix readout base 56. The turntable motor 531 drives the reaction cup turntable 52 to rotate through a pulley mechanism, wherein the turntable motor 531 drives the turntable driving pulley 532, the turntable driven pulley 533 is fixed on the reaction cup turntable 52, and the turntable driving pulley 532 drives the turntable driven pulley 533 to rotate through the turntable synchronous belt 534. The turntable drive mechanism includes a first limit optocoupler 535 for detecting the rotation angle of the cuvette turntable 52. Besides the adoption of a limiting optocoupler, a rotary encoder can be added to the output shaft of the turntable motor to calculate the rotation angle of the reaction cup turntable 52.

The output shaft of the shaking motor 551 is provided with a shaking driving wheel 552, the shaking piece 54 is fixed with a shaking driven wheel 553, and the shaking driven wheel 553 is in transmission connection with the shaking driving wheel 552 through a shaking synchronous belt 554.

A mask support column 513 is provided on the mix readout base 56, and a mask 514 is fixed to the mask support column 513 and above the photomultiplier tube 5102. The mask 514 is provided with a reaction cup avoiding hole 5141, when the pipetting device 10 needs to take and place the reaction cup 6, the reaction cup hole 522 corresponding to the reaction cup turntable 52, the reaction cup avoiding hole 5141 and the reaction cup taking and placing hole 571 on the mask 57 are correspondingly overlapped, and a relatively closed cued environment can be formed by the mask 57, the mask 514 and the baffle 582, so that the reading stability of the photomultiplier 5102 is ensured.

The working principle of the mixing reading module 5 is as follows: the reagent cup 6 is added with a special reagent for detecting sample items by other modules in the whole chemiluminescence process, substrate liquid is added after the reagent cup 6 is subjected to cleaning magnetic separation by the cleaning device module 4, the three-dimensional arm module 8 is matched with the pipetting device 10 to transfer the reagent cup 6 of the cleaning device module 4 to the position right above the reagent cup taking and placing hole 571, after the rotary driving mechanism 581 rotates clockwise to drive the baffle 582 to open, the pipetting device 10 places the reagent cup 6 into the reagent cup hole 522 in the reagent cup turntable 52 from the reagent cup taking and placing hole 571, then the rotation position and angle are controlled by a photoelectric switch, the reagent cup turntable 52 rotates a hole position, the pipetting device 10 picks up the reagent cup 6 after the previous round of testing, and at the moment, the rotary driving mechanism 581 rotates anticlockwise to drive the baffle 582 to stay right above the reagent cup 6. After the reaction cup turntable 52 rotates by one hole site, the reaction cup 6 enters the shaking accommodating space 541, the shaking motor 551 starts to operate, and the shaking piece 54 is driven to rotate through the shaking driving wheel 552, the shaking synchronous belt 554 and the shaking driven wheel 553 which are connected, so that liquid in the reaction cup 6 is driven to perform reciprocating mixing and shaking actions. After the mixing action, the turntable motor 531 drives the reaction cup turntable 52 to continuously rotate by one hole position, so that the detection hole of the photomultiplier tube 5102 can directly read the value of the luminous value of the reagent in the reaction cup 6, the photomultiplier tube 5102 detects the luminous value of the reagent, and the luminous value is output to the PC end for result conversion and then the detection result is output. When one reaction cup 6 leaves the shaking accommodating space 541 and goes to the reading area for reading, one reaction cup 6 enters the shaking accommodating space 541 at the same time, and when the reading module 51 reads a value of one reaction cup 6, the shaking member 54 also performs a shaking operation on one reaction cup 6 at the same time.

After the reading module 51 finishes reading, the rotary driving mechanism 581 rotates the driving baffle 582 clockwise to return to the initial position, after the next new cuvette 6 to be detected is placed in the position of the cuvette taking hole 571, the cuvette turntable 52 rotates again by an angle of one hole, and the pipetting device 10 takes out the detected cuvette 6 and transfers it to the waste box 72 (see fig. 15 and 16), and then the whole mixing reading period is completed.

In one embodiment, referring to fig. 1 and 2 and fig. 8 to 10, the sample processing module 2 includes a sample tube rack module 21, a sample tube rack box 22, and a tube rack driving mechanism, the sample tube rack module 21 includes a tube rack seat 211 and a sample tube rack 212 rotatably disposed on the tube rack seat 211, the tube rack driving mechanism drives the sample tube rack 212 to rotate, and the sample tube rack 212 has a sample tube accommodating space for accommodating sample tubes. The sample tube rack module 21 is inserted in the sample tube rack box 22, and a tube rack driving mechanism is arranged on the sample tube rack box 22, and the tube rack driving mechanism comprises a driving motor 231 and a driving gear train. Be fixed with driven gear 213 on the sample pipe support 212, drive gear train includes drive gear 232, transfer gear 233 and intermediate gear 234, and transfer gear 233 and sample pipe support module 21 one-to-one correspond, in the same sample pipe support module 21: driven gears 213 on adjacent sample racks 212 mesh. The transmission gear 233 is engaged with at least one driven gear 213 in the corresponding sample tube rack module 21; the driving gear 232 is engaged with at least one intermediate gear 234, and the intermediate gear 234 is simultaneously engaged with the adjacent driving gears 233, so that the driving gear 232 rotates each driving gear 233.

Specifically, as shown in fig. 8 and 9, the sample processing module 2 includes a sensor 24 and a motor mount 25.

The sample tube rack module 21 includes a bearing block 214 and a sample tube spring 215. The sample tube spring 215 is used to hold the sample tube. The rack holder 211 is provided with a handle grip hole 2111 for inserting the hand-operated sample rack module 21 into the take-out sample rack box 22.

The bearing housing 214 has a tube rack mounting groove 2141, a bearing housing boss 2142 is provided at the front end of the bearing housing 214, and the sample tube rack 212 has a sample tube receiving groove 2121. When the sample tube rack module 21 is inserted into the sample tube rack box 22, the bearing pedestal boss 2142 passes through the sensor 24, the sensor 24 detects an insertion signal and transmits the insertion signal to the computer control end, so that the instrument can master sample loading information at any time, and a specified command is sent to enable the driving motor 231 to start to operate.

As shown in fig. 10, the motor housing 25 is provided with a motor shaft through hole 2501, the driving motor 231 is fixed to the motor housing 25, the motor shaft of the driving motor 231 is penetrated out from the motor shaft through hole 2501, and the driving gear 232 is fixed to a portion of the motor shaft penetrated out from the motor shaft through hole 2501. The motor base 25 is provided with 5 gears, namely a first intermediate gear 2341, a first transmission gear 2331, a second transmission gear 2332, a second intermediate gear 2342 and a third transmission gear 2333. The drive gear 232 is meshed with a first intermediate gear 2341, the first intermediate gear 2341 is meshed with a first transmission gear 2331 and a second transmission gear 2332 respectively, the second transmission gear 2332 is meshed with a second intermediate gear 2342, and the second intermediate gear 2342 is meshed with a third transmission gear 2333. The sample processing module 2 is provided with 3 sample tube rack modules 21, and each sample tube rack module 21 is provided with 10 driven gears 213 which are arranged in a queue and meshed with each other. When the three sample tube rack modules 21 are properly mounted, the first drive gear 2331 is engaged with the driven gear 213 of the first sample tube rack module 21, the second drive gear 2332 is engaged with the driven gear 213 of the second sample tube rack module 21, and the third drive gear 2333 on the motor mount 25 is engaged with the driven gear 213 of the second sample tube rack module 21. When the driving motor 231 is operated, the driving gear 232 drives the first intermediate gear 2341, the first transmission gear 2331, the second transmission gear 2332, the second intermediate gear 2342 and the third transmission gear 2333 to rotate, the first transmission gear 2331 drives each driven gear 213 of the first sample tube rack module 21 to rotate, the second transmission gear 2332 drives each driven gear 213 of the second sample tube rack module 21 to rotate, and the third transmission gear 2333 drives each driven gear 213 of the third sample tube rack module 21 to rotate. The sample tube rack 212 can be driven to synchronously do circular reciprocating motion through reversing rotation of the driving motor 231, so that blood in the sample tube is fully and uniformly mixed, and layering can not occur.

In one embodiment, referring to fig. 11 to 13, the chemiluminescent analyzer comprises a substrate refrigeration assembly 9, the substrate refrigeration assembly 9 comprises a refrigeration box 91 and a refrigeration module 92 for refrigerating the refrigeration box 91, the rack 1 is provided with a reagent taking and placing door (not shown in the drawings), the refrigeration box 91 is provided with a refrigeration box side opening 911, the refrigeration box side opening 911 faces the reagent taking and placing door for a substrate bottle 94 to enter and leave the refrigeration box 91, a refrigeration box frame 93 is arranged at the top of the refrigeration box 91, and the refrigeration box frame 93 is used for hanging the substrate bottle 94. After the substrate bottle 94 and the refrigerating box frame 93 of the refrigerating box 91 are matched, a certain gap is reserved between the bottom of the inner side of the refrigerating box 91 and the bottommost surface of the substrate bottle 94, so that the condensed water can be conveniently discharged in a flowing mode.

Further, referring to fig. 11 and 12, the opening 911 of the side of the refrigeration cassette is located at the front side of the refrigeration cassette 91, a left support plate 912 for supporting the left end of the refrigeration cassette rack 93 is provided on the left side wall of the refrigeration cassette 91, a right support plate 913 for supporting the right end of the refrigeration cassette rack 93 is provided on the right side wall of the refrigeration cassette 91, and the left support plate 912 is gradually inclined downward from left to right, and the right support plate 913 is gradually inclined downward from right to left. This allows the condensed water formed by the heat exchange to smoothly flow down on the inclined surface by gravity, preventing the condensed water from accumulating on the surface of the supporting structure and carrying out the condensed water when the substrate bottle 94 is replaced. Also make when changing substrate bottle 94, can hang substrate bottle apron 95 temporarily on this cold-stored box frame 93, prevent the pipeline pollution and conveniently change substrate liquid, cold-stored box frame 93 all goes into the line face cooperation with right side backup pad 913, left side backup pad 912, reduces the area of contact each other.

Specifically, referring to fig. 11 to 13, the substrate cooling assembly 9 includes a temperature sensor 96, a fuse 97, and thermal insulation foam. The refrigerating module 92 is tightly attached to the rear position of the refrigerating box 91 through heat-conducting silicone grease to provide refrigeration, the temperature sensor 96 is mounted on the side position of the refrigerating cavity 91, and the fuse 97 is mounted on the heat-conducting plate in front of the refrigerating module 92 to detect the temperature of the heat-conducting aluminum block. The heat preservation sponge is 920, heat preservation foam is 921 down, heat preservation foam left 922, heat preservation foam right 923, heat preservation foam rear 924 paste respectively in the position of "upper", "lower", "left", "right", "rear" of refrigeration cavity 91, and substrate bottle 94 is installed in the inside position of refrigeration cavity 91, and heat preservation foam plays effective heat preservation effect to refrigeration cavity internal environment.

The substrate bottle cap 95 is sealed to the substrate bottle 94 by a gasket 98. The sealing ring 98 is arranged below the substrate bottle cover plate 95 and is integrally matched with the substrate bottle 94, and the sealing ring 98 plays a role in sealing, so that condensed water is effectively prevented from entering the substrate bottle 94, and pollution is prevented. Meanwhile, the substrate bottle cover plate 95 is in compression fit with the substrate bottle 94, and compared with threaded fit, the operation is simpler and more convenient, and the improvement of the operation efficiency is facilitated.

In one embodiment, referring to fig. 12-14, a substrate bottle cover 95 is fixedly attached to the refrigeration cassette rack 93. The substrate bottle cap plate, the substrate bottle cap plate 95, the refrigeration cassette rack 93, the substrate bottle 94 and the sealing ring 98 are assembled and integrally put into the refrigeration cassette. The substrate bottle cover plate 95 is fixed with the refrigerating box frame 93 by screws. In some other embodiments, the substrate bottle cover 95 and the refrigerated case frame 93 may be snapped together to facilitate removal of the substrate bottle cover 95 from the refrigerated case frame 93.

The drain hole 9102 is arranged at the lowest position of the inner bottom surface of the refrigeration box 91, the inclined surface 9101 with uniform downward inclination direction is arranged on the inner bottom surface of the refrigeration box 91 and is collected in the drain hole 9102, the collection structure of the inclined surface 9101 with the same direction is beneficial to drainage of condensed water flowing into the drain hole, backflow is effectively prevented, high-speed drainage is realized, meanwhile, the production cost is low, and the economic benefit is good. Further, the drain hole 9102 is a threaded hole, and the elbow 99 is screwed into the drain hole. The water suction pump can be connected to the outside of the elbow 99, and condensed water can be quickly discharged through the switch control water pump, so that the phenomenon that the condensed water gathers too much and converges to the inside of an instrument to cause short circuit of a circuit and pollute the inside of the instrument is prevented.

Referring to fig. 11 and 13, the rear side wall of the refrigeration box 91 has a limiting slot 914 for the substrate bottle to enter, and the limiting slot 914 is used for limiting the substrate bottle to swing left and right, so as to ensure the stability and accuracy of the substrate bottle 94 during assembly and effectively improve the operation efficiency.

The refrigerating box 91 is made of aluminum alloy, and has good thermal conductivity, easy processing and good economic benefit. The temperature sensor 96 is installed at the rear position outside the refrigeration box 91, and plays a role in monitoring the temperature environment inside the refrigeration box 91.

Referring to fig. 11 and 12, a radiator is installed behind the heat conducting plate of the refrigeration module 92, and the radiator is used for radiating heat from the refrigeration module 92. The front side of the heat conducting plate is attached to the refrigerating sheet of the refrigerating module 92, so that heat generated by the refrigerating sheet is led out, and the fuse 97 is installed in front of the heat conducting plate, when the temperature of the heat conducting plate reaches a certain height, the fuse 97 is fused, and therefore the whole circuit can be cut off, and the protection function is achieved.

In one embodiment, referring to fig. 1, 15 and 16, the waste cartridge module 7 includes a housing 71, a guide 75 extending through the housing 71, and a waste cartridge 72 secured to the outside of the housing 71. The end of the guide 75 extending into the housing 71 has a waste receiving opening 751, and the end extending out of the housing 71 has a waste outlet 752, and the waste cartridge 72 is positioned below the waste outlet 752 for receiving waste falling from the guide 75.

Specifically, the chemiluminescent analyzer comprises a waste box carrier 73 and a waste box cover 74, the shell 71 comprises a right side plate, the reaction cup 6 or other waste materials are returned at a waste receiving port 751 of the material carrying part, and an inclined surface is arranged on the bottom surface of the waste material outlet 752, so that the waste materials more smoothly slide into the waste box 72 under the action of gravity. The waste box 72 is placed inside the waste box carrier 73, and the waste box carrier 73 is installed on one side of the right side plate and placed at an outlet position below the waste outlet 752. The waste box cover plate 74 is arranged on the upper surface of the waste box bearing frame 73 and covers the waste box 72, the waste box cover plate 74 is made of transparent materials, the internal loading of the waste box 72 can be observed, a convex wrapping flange is designed on the upper part of the waste box cover plate 74, the waste outlet 752 can be wrapped, the waste can be effectively prevented from being accidentally ejected out of the waste box 72 when the waste falls, and meanwhile, the waste is prevented from being directly exposed to the surface to influence the appearance and reduce the biohazard.

In one embodiment, please refer to fig. 1, the chemiluminescent analyzer is a fully automated chemiluminescent analyzer, which comprises a sample processing module 2, an incubation module 3 for placing a reaction cup and providing an incubation reaction environment for reagents in the reaction cup, a washing device module 4 for washing magnetic beads, a mixing read module 5, a waste box module 7, a three-dimensional arm module 8 for moving reagents, samples, reaction cups, a substrate refrigeration assembly 9, a pipetting device 10 for sucking and transferring reagents, samples and mixing, a reaction module 11 for testing each reagent and sample reaction in the reaction process, a washing liquid and waste liquid barrel 12 for placing washing liquid and waste liquid, a scanning module 13 for reading test strip information, a reagent processing module 14, a TIP head module 15 for placing a TIP head, and an electronic control part and a computer control end. The incubation module 3, the washing device module 4, the pipetting device 10, the reaction module 11, the scanning module 13, the reagent processing module 14, the TIP head module 15, and the electronic control unit and the computer control terminal may be any available methods in the prior art, for example, refer to the disclosure in the grant publication No. CN 218412570U. The three-dimensional arm module 8 may refer to the disclosure in CN216322024U, and will not be described in detail in this application.

In one embodiment, the cleaning solution and waste liquid tank 12 is located outside the housing 1, which makes the space inside the housing 1 more compact.

In one embodiment, the detection operation flow of the full-automatic chemiluminescence determinator is as follows: selecting an item to be tested on a screen; sample tube rack module 21 is provided with 30 sample locations where single or multiple samples can be tested simultaneously. The test items can be selected from single or a plurality of different combined items, and each test sample position has no interference effect;

the sample tube is manually clamped on the sample tube rack and then integrally inserted to the corresponding position of the sample processing module 2, the scanning module 13 can synchronously scan the information on the sample tube while the sample tube rack is inserted, then the reagent kit is placed at the bearing position of the reagent kit in the instrument, the TIP head is placed on the TIP head module, the reaction cup is placed on the incubation module, the bin gate is closed, and the item is selected to be clicked to start the test. The test process is fully automatic, and comprises the following steps: sample frame test information scanning code reading, automatic TIP head taking, reagent and sample sucking to a reaction cup, reagent incubation, reagent mixing (mixing in an absorbing and spitting mode), magnetic particle cleaning and separation, substrate adding, reaction cup moving to a mixing reading module for vibration mixing and luminous value reading, test result outputting, and waste box discarding of the tested reaction cup and TIP head.

The foregoing description of the invention has been presented for purposes of illustration and description, and is not intended to be limiting. Several simple deductions, modifications or substitutions may also be made by a person skilled in the art to which the invention pertains, based on the idea of the invention.

Claims

1. The chemiluminescent analyzer is characterized by comprising a rack, a sample processing module, an incubation module, a cleaning device module and a mixing reading module; the mixing reading module is used for mixing the reagents uniformly and detecting the luminescence value of the reaction; the blending read value module comprises:

2. The chemiluminescent apparatus of claim 1 wherein the shaking element is rotatably disposed on the frame, the shaking drive mechanism drives the shaking element to rotate, and the shaking element acts on the reaction cup to shake the reaction cup during rotation.

3. The chemiluminescent apparatus of claim 2 wherein the axis of rotation of the shaking element is spaced parallel to the centerline of the cuvette well.

4. The chemiluminescent apparatus of claim 1 wherein the shake-up containment space has a first opening and a second opening; when the reaction cup moves to the shaking accommodating space along with the reaction cup turntable, one of the first opening and the second opening is used for the reaction cup to enter the shaking accommodating space, and the other one is used for the reaction cup to leave the shaking accommodating space.

5. The chemiluminescent apparatus of claim 4 wherein the shaking element has a shaking slot, the space in the shaking slot forming the shaking receiving space, the shaking slot having a slot bottom wall, a first slot side wall and a second slot side wall, the shaking slot having a top opening opposite the slot bottom wall, the first opening and the second opening each communicating with the top opening; the shaking-up piece shakes the reaction cup through the side wall of the first groove and/or the side wall of the second groove.

6. The chemiluminescent apparatus of any one of claims 1-5 wherein the blending read module comprises a base disposed on the frame and a light shield covering the upper side of the base, the light shield and base enclosing a darkroom, the reaction cup turntable, the read module and the shaking piece all being in the darkroom; the shaking driving mechanism is arranged at the lower side of the base, and the shaking piece is arranged at the upper side of the base.

7. The chemiluminescent apparatus of any one of claims 1-5 wherein the chemiluminescent apparatus comprises a substrate refrigeration assembly comprising a refrigerated case and a refrigeration module that refrigerates the refrigerated case, the refrigerated case having a refrigerated case side opening into and out of the refrigerated case, the refrigerated case top having a refrigerated case shelf for lifting a substrate bottle;

8. The chemiluminescent apparatus of claim 7 wherein the refrigerated case side opening is on the front side of the refrigerated case and the rear side sidewall of the refrigerated case has a limiting slot for access to a substrate bottle, the limiting slot having a means for limiting side-to-side rocking of the substrate bottle.

9. The chemiluminescent apparatus of any one of claims 1-5 wherein the chemiluminescent apparatus comprises a housing, a guide extending through the housing, a waste cartridge affixed to the outside of the housing; the guide piece stretches into one end in the shell is provided with a waste receiving port, one end stretching out of the shell is provided with a waste outlet, and the waste box is positioned at the lower side of the waste outlet and is used for receiving waste falling from the guide piece.

10. The chemiluminescent assay of any one of claims 1-5 wherein the sample processing module comprises a sample tube rack module, a sample tube rack cartridge, and a tube rack drive mechanism, the sample tube rack module comprising a tube rack mount and a sample tube rack rotatably disposed on the tube rack mount, the tube rack drive mechanism driving rotation of the sample tube rack, the sample tube rack having a sample tube receiving space for receiving a sample tube; sample pipe support module cartridge is in the sample pipe support box, pipe support actuating mechanism disposes on the sample pipe support box, pipe support actuating mechanism includes driving motor and drive gear train, be fixed with driven gear on the sample pipe support, drive gear train includes drive gear, drive gear and intermediate gear, drive gear with sample pipe support module one-to-one, in the same sample pipe support module: driven gears on adjacent sample tube frames are meshed; the transmission gear is meshed with at least one driven gear in the corresponding sample pipe rack module; the driving gear is meshed with at least one intermediate gear, and the intermediate gear is meshed with the adjacent transmission gears simultaneously, so that the driving gear drives each transmission gear to rotate.