CN117464760B

CN117464760B - Intelligent tissue slice culture device

Info

Publication number: CN117464760B
Application number: CN202311507874.1A
Authority: CN
Inventors: 修阳晖; 张玉洁; 苏盛齐
Original assignee: Xiamen Eye Center Co ltd
Current assignee: Xiamen Eye Center Co ltd
Priority date: 2023-11-14
Filing date: 2023-11-14
Publication date: 2024-04-19
Anticipated expiration: 2043-11-14
Also published as: CN117464760A

Abstract

The invention discloses an intelligent tissue slice culturing device, which relates to the technical field of slice culturing and comprises a processing component for carrying out early-stage processing and later-stage collection on a plant sample, wherein the processing component flattens the sample and improves the cutting effect of the sample, a wiping component for cleaning the sample is arranged on the side edge of the processing component, the surface of the sample is wiped by the wiping component, the observation effect of the sample is improved, a cutting component for cutting the sample is arranged on the side edge of the processing component, the cutting component automatically controls the cutting size of the sample, the sample is cut into different sizes according to different observation requirements, and meanwhile, cut wastes can be automatically cleaned, so that the waste sample is prevented from entering a culture dish.

Description

Intelligent tissue slice culture device

Technical Field

The invention relates to the technical field of slice culture, in particular to an intelligent tissue slice culture device.

Background

The observation of plant sections provides the opportunity for deep knowledge of plant tissue structure and function, as well as valuable information for a variety of scientific and application fields, by growing plant tissue sections, researchers can explore plant physiology and developmental processes including cell division, tissue expansion, cell differentiation, etc. This helps to reveal the growth mechanism of plants, while the culture section can be used to study interactions between plants and pathogens, pests, to better understand the mechanisms of plant disease and pest resistance.

The Chinese patent publication No. CN215328156U discloses a slice tissue culture device, which adds culture solution to a culture dish under the condition of not opening a box body, and simultaneously, the device automatically places the culture dish, but the device cannot ensure the interval between sample strips and cannot clean residual samples.

Disclosure of Invention

In view of the problems in the prior art, the invention provides an intelligent tissue slice culture device.

Aiming at the technical problems, the invention adopts the following technical scheme: the utility model provides a tissue slice intelligent culture device, includes processing subassembly, the processing subassembly include the base, the base on be provided with movable plate, pressing plate, support frame, carriage and ejection of compact board, place the sample on the movable plate, pressing plate presses the blade on the movable plate, processing subassembly on still be provided with and wipe subassembly and cutting subassembly, the subassembly of cleaning clean the sample on the movable plate, the cutting subassembly cut the sample on the movable plate, ejection of compact board collect the sample of accomplishing to the cutting, the subassembly of cleaning include that slidable mounting is on the first crane of support frame, first crane on be provided with adjustment mechanism and clean the head, adjustment mechanism control the position of cleaning the head, the cutting subassembly including slidable mounting cut the board on the base, the cutting board on be provided with cutting knife one, cutting knife two and clearance mechanism, cutting knife two clear up the sample, the mechanism clear up the sample.

Further, the base on be provided with multiunit clearance platform, clearance bench on be provided with multiunit sprinkler head one with press the post, sprinkler head a pair of sample surface wash, press the post and press the sample.

Further, the discharging plate on be provided with sprinkler head two and strike the board, strike board and discharging plate sliding connection, strike the board and strike the discharging plate, promote dropping of the material on the discharging plate.

Further, the base on rotate and be provided with the rotating turret, the rotating turret on be provided with multiunit culture dish.

Further, adjustment mechanism include lifting disk and the pivoted post one of installing on lifting frame one, pivoted post one on rotate and be provided with pivoted post two, pivoted post two one end be provided with and wipe the head, pivoted post two other end be provided with spacing ball, spacing ball and lifting disk sliding connection, the lifting disk realizes the regulation of wiping the head position when sliding on lifting frame one.

Further, the cutting board on slide and be provided with the sliding table, cutting knife one with sliding connection, sliding table and cutting knife one between be provided with the spring four, the sliding table on rotate and be provided with the cam two, the cam two drive cutting knife one and cut the sample.

Further, clearance mechanism include that slidable mounting is provided with the spring between crane two and the cutting board on the cutting board two, crane two lower extreme is provided with divides the work or material rest, divides the work or material rest to slide and is provided with the lifter, rotates on the lifter and is provided with the discharging roller, lifter and divide and be provided with the spring between the work or material rest three, discharging roller and sample press.

Further, the cutting assembly further comprises a pushing rod which is slidably mounted on the sliding frame, and the pushing rod pushes the sample cut on the moving plate to the discharging plate.

Compared with the prior art, the invention has the beneficial effects that: (1) The processing component provided by the invention is used for preprocessing the sample before cutting and recovering the sliced sample, so that the cleanliness of the sample is ensured, the collection efficiency of the sample is improved, and the scattering of the sample is avoided; (2) The wiping component wipes the surface of the sample, so that the phenomenon that the quality of the sliced sample is changed during cultivation and the observation is wrong or the sliced tissue dies due to stubborn stains on the sample is avoided; (3) The cutting assembly provided by the invention automatically slices the sample, and the size of the slice is automatically adjusted according to different requirements, so that the invention is adaptive, and meanwhile, the cutting assembly can automatically clean the abandoned sample after slicing, so that the slicing efficiency and slicing effect of the cutting assembly are improved.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present invention.

Fig. 2 is a top view of the overall structure of the present invention.

FIG. 3 is a schematic view of a processing assembly according to the present invention.

FIG. 4 is a schematic view of a cleaning station according to the present invention.

Fig. 5 is a front view of the discharge plate structure of the present invention.

Figure 6 is a schematic view of the structure of a wiping assembly of the present invention.

Figure 7 is a cross-sectional view of a wiping assembly of the present invention.

Figure 8 is a schematic view of a partial construction of a wiping assembly of the present invention.

FIG. 9 is a schematic view of a cutting assembly according to the present invention.

FIG. 10 is a schematic view of a partial structure of a cutting assembly according to the present invention.

Fig. 11 is a partial structural cross-sectional view of the cutting assembly of the present invention.

Fig. 12 is an enlarged schematic view of the structure a in fig. 11.

Reference numerals: 1-a processing assembly; 2-a wiper assembly; 3-a cutting assembly; 101-a base; 102-pressing the plate; 103-cleaning a table; 104-a supporting frame; 105-a carriage; 106, a discharging plate; 107-rotating frame; 108-a culture dish; 109-mounting rack; 110-a mobile plate; 111-first sprinkler; 112-pressing the column; 113-second sprinkler; 114-cam one; 115—strike plate; 116-spring one; 201-lifting frame I; 202-lifting disc; 203-a wiper head; 204-swinging the rod; 205-turning column one; 206-rotating the second column; 207-limit ball; 301-cutting a plate; 302, lifting frame II; 303-spring two; 304-a sliding table; 305-cam two; 306-push rod; 307-cutter one; 308-a material separating frame; 309-second cutter; 310-lifting blocks; 311-spring three; 312-discharge roller.

Detailed Description

Referring to fig. 1 to 12, an intelligent tissue slice culturing device comprises a processing assembly 1 for performing pretreatment and later collection on a plant sample, a wiping assembly 2 for cleaning the sample is arranged on the side edge of the processing assembly 1, and a cutting assembly 3 for cutting the sample is further arranged on the side edge of the processing assembly 1.

The processing component 1 comprises a base 101, a pressing plate 102 and a moving plate 110 are arranged on the base 101 in a sliding manner, the moving plate 110 is used for placing samples to be cut, when the pressing plate 102 slides on the base 101, the pressing plate 102 presses the samples on the moving plate 110 to ensure the flatness of the samples, a supporting frame 104 and a sliding frame 105 are also arranged on the base 101, a wiping component 2 is arranged on the supporting frame 104, cleaning tables 103 are also arranged on two sides of the supporting frame 104, a plurality of groups of first spraying heads 111 and first pressing columns 112 are arranged on the cleaning tables 103, the first pressing columns 112 are in sliding connection with the cleaning tables 103, the first spraying heads 111 flush the samples, the first pressing columns 112 press the samples to ensure the stability of the samples when the wiping component 2 wipes the samples, the cut samples are pushed into the discharging plates 106, the discharge plate 106 is rotatably mounted on the base 101, a mounting frame 109 is arranged on the discharge plate 106, a plurality of groups of spraying heads II 113 are arranged on the mounting frame 109, the spraying heads II 113 spray culture solution into the discharge plate 106, the culture solution and a cut sample enter the culture dish 108 together, a knocking plate 115 is slidably arranged at the lower end of the discharge plate 106, a first spring 116 is arranged between the knocking plate 115 and the discharge plate 106, a first cam 114 is rotatably arranged on the discharge plate 106, the first cam 114 drives the knocking plate 115 to reciprocate on the discharge plate 106, the knocking plate 115 knocks the discharge plate 106, the dropping speed of materials in the discharge plate 106 is improved, a rotating frame 107 is rotatably arranged on the base 101, a plurality of groups of culture dishes 108 are arranged on the rotating frame 107, and the rotating frame 107 drives the culture dish 108 to be aligned with the discharge plate 106.

The wiping component 2 comprises a first lifting frame 201 which is slidably arranged on the supporting frame 104, a first rotating column 205 is rotatably arranged on the first lifting frame 201, a second rotating column 206 and a swinging rod 204 are rotatably arranged in the first rotating column 205, a wiping head 203 is arranged at one end of the second rotating column 206, a limit ball 207 is arranged at the other end of the second rotating column 206, a lifting plate 202 is slidably arranged on the first lifting frame 201, the upper end of the lifting plate 202 is slidably connected with the limit ball 207, the lower end of the lifting plate 202 is slidably connected with the swinging rod 204, when the swinging rod 204 is driven to rotate on the first lifting frame 201, the swinging rod 204 drives the lifting plate 202 to slide on the first lifting frame 201, the lifting plate 202 drives the limit ball 207 to move, the limit ball 207 drives the wiping head 203 to rotate along the axis of the second rotating column 206, when the first rotating column 205 is driven to rotate, the first rotating column 205 drives the second rotating column 206 to rotate along the axis of the first rotating column 205, and the wiping head 203 is regulated.

The cutting assembly 3 comprises a pushing rod 306 which is slidably arranged on the sliding frame 105, and further comprises a cutting plate 301 which is slidably arranged on the base 101, wherein a lifting frame II 302 and a sliding table 304 are slidably arranged on the cutting plate 301, a cutting knife I307 is slidably arranged on the sliding table 304, a spring II is arranged between the cutting knife I307 and the sliding table 304, a cam II 305 is rotatably arranged on the sliding table 304, the cutting knife I307 is driven to reciprocally slide on the sliding table 304 during rotation of the cam II 305, the lower end of the cutting knife 307 cuts a sample, the sample is cut into a required strip by moving the sliding table 304, the sample is conveniently observed, a cutting knife II 309 is arranged at the lower end of the cutting plate 301, a cutting knife II 309 is corrected to cut the sample into a rectangle, then the sample is cut by the cutting knife I307, a spring II 303 is arranged between the lifting frame II 302 and the cutting plate 301, a material dividing frame 308 is arranged at the lower end of the lifting frame II, a lifting block 310 is slidably arranged in the dividing frame 308, a spring III 311 is arranged between the lifting block 310 and the dividing frame 308, a discharging roller 312 is rotatably arranged on the lifting block 310, and the discharging roller is pressed against the sample 309, and the sample is prevented from falling off the sample 309 when the sample is cut.

Working principle: when the sample cleaning device works, a sample to be sliced is placed on the moving plate 110, the moving plate 110 is driven to slide on the base 101, when the moving plate 110 drives the sample to reach the lower part of the pressing plate 102, the pressing plate 102 is driven to descend, the pressing plate 102 presses the sample on the moving plate 110, the flatness of the sample is improved, the moving plate 110 is driven to move continuously, when the moving plate 110 passes through the first group of cleaning tables 103, the first spraying heads 111 on the cleaning tables 103 are driven to flush the sample, when the sample reaches the lower end of the supporting frame 104, the pressing columns 112 on the two groups of cleaning tables 103 are driven to synchronously descend, the pressing columns 112 press the sample on the moving plate 110, the first lifting frame 201 is driven to slide on the supporting frame 104, the first lifting frame 201 drives the wiping head 203 to descend, the wiping head 203 is attached to the sample, the first rotating column 205 is driven to rotate, the second rotating column 206 and the wiping head 203 rotate along the axis of the first rotating column 203, when the position of the wiping head 203 is required to be adjusted, the first swinging rods 204 drive the first lifting rods 204 to rotate on the lifting frame 201, the first lifting rods 204 drive the second lifting rods 202 to slide on the first lifting frame 202 to drive the second lifting frame 203 to slide along the axis of the second lifting frame 203, and the second lifting frame 203 to move the second lifting rods to move the first lifting rods 203 along the axis of the sliding heads 203.

After the sample on the moving plate 110 is wiped, the moving plate 110 is driven to move below the cutting assembly 3, the cutting plate 301 is driven to slide on the base 101, the cutting plate 301 moves towards the direction close to the moving plate 110, when the cutting plate 301 descends, the material separating frame 308 is firstly contacted with the sample on the moving plate 110, the cutting plate 301 continues to descend, the material separating frame 308 presses the sample on the moving plate 110, meanwhile, the discharging roller 312 and the lifting block 310 are pressed in the material separating frame 308, at the moment, the discharging roller 312 is contacted with the sample, at the moment, the lifting frame two 302 slides on the cutting plate 301, meanwhile, the spring two 303 is pulled away, the cutting plate 301 continues to drive the cutting blade two 309 to approach the sample, the cutting blade two 309 cuts the sample into a cuboid when contacting the sample, then the cam two 309 is driven to rotate, the cutting blade one 307 is driven to slide on the sliding table 304, the cutting blade one 307 cuts the sample, the distance of the sliding table 304 is driven to move on the cutting plate 301, and the size of the cutting blade 307 cut sample is changed.

After cutting is completed, the cutting plate 301 is reset, when the material dividing frame 308 leaves the sample and the material discharging roller 312 presses the sample, the driving of the cutting plate 301 is stopped, the first cutting knife 307, the second cutting knife 309 and the material dividing frame 308 are all in contact with the sample, at this time, the material discharging roller 312 contacts with the residual sample, at the same time, the two groups of material discharging rollers 312 are driven to rotate, the residual sample is broken when the material discharging roller 312 rotates, the residual sample is discharged from two sides of the moving plate 110, at this time, only the sample with the cut slice is left on the moving plate 110, then the cutting plate 301 is driven to be completely reset, the pushing rod 306 is driven to slide on the sliding frame 105, and the sliding frame 105 pushes the sample on the moving plate 110 into the material discharging plate 106.

The discharge plate 106 is driven to rotate on the base 101, meanwhile, the second spraying head 113 is driven to spray culture solution into the discharge plate 106, the rotating frame 107 is driven to rotate, the rotating frame 107 drives the culture dish 108 to be aligned with the discharge plate 106, at the moment, a sample obtained by mixing and slicing the nutrient solution enters the culture dish 108, meanwhile, the first cam 114 is driven, the first cam 114 drives the knocking plate 115 to knock the discharge plate 106, and the descending speed of materials in the discharge plate 106 is improved.

The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope of the present invention without inventive labor, as those skilled in the art will recognize from the above-described concepts.

Claims

1. The utility model provides a tissue slice intelligent culture device, includes processing assembly (1), its characterized in that: the processing component (1) comprises a base (101), the base (101) is provided with a moving plate (110), a pressing plate (102), a supporting frame (104), a sliding frame (105) and a discharging plate (106), a sample is placed on the moving plate (110), the pressing plate (102) presses a blade on the moving plate (110), the processing component (1) is also provided with a wiping component (2) and a cutting component (3), the wiping component (2) wipes the sample on the moving plate (110), the cutting component (3) cuts the sample on the moving plate (110), the discharging plate (106) collects the cut sample, the wiping component (2) comprises a lifting frame I (201) which is slidably arranged on the supporting frame (104), an adjusting mechanism and a cutting head (203) are arranged on the lifting frame I (201), the adjusting mechanism controls the position of the wiping head (203), the cutting component (3) comprises a cutting mechanism I (307) which is slidably arranged on the base (101), a cutting mechanism II (307) and a cutting knife II (309) are arranged on the cutting plate (307), the cleaning mechanism is used for cleaning the waste samples;

The cutting device is characterized in that a sliding table (304) is arranged on the cutting plate (301) in a sliding manner, the first cutting knife (307) is connected with the sliding table (304) in a sliding manner, a spring IV is arranged between the sliding table (304) and the first cutting knife (307), a second cam (305) is arranged on the sliding table (304) in a rotating manner, and the second cam (305) drives the first cutting knife (307) to cut a sample;

The cleaning mechanism comprises a second lifting frame (302) which is slidably arranged on the cutting plate (301), a second spring (303) is arranged between the second lifting frame (302) and the cutting plate (301), a material separating frame (308) is arranged at the lower end of the second lifting frame (302), a lifting block (310) is slidably arranged in the material separating frame (308), a discharging roller (312) is rotatably arranged on the lifting block (310), a third spring (311) is arranged between the lifting block (310) and the material separating frame (308), and the discharging roller (312) is pressed with a sample;

The cutting assembly (3) further comprises a pushing rod (306) which is slidably arranged on the sliding frame (105), and the pushing rod (306) pushes the cut sample on the moving plate (110) to the discharging plate (106).

2. The intelligent tissue slice culture device according to claim 1, wherein: the base (101) on be provided with multiunit clearance platform (103), clearance platform (103) on be provided with multiunit sprinkler head one (111) and press post (112), sprinkler head one (111) wash the sample surface, press post (112) to press the sample.

3. The intelligent tissue slice culture device according to claim 1, wherein: the discharging plate (106) is provided with a second sprinkler head (113) and a knocking plate (115), the knocking plate (115) is in sliding connection with the discharging plate (106), the knocking plate (115) knocks the discharging plate (106) to promote the falling of materials on the discharging plate (106).

4. The intelligent tissue slice culture device according to claim 1, wherein: the base (101) is rotatably provided with a rotating frame (107), and the rotating frame (107) is provided with a plurality of groups of culture dishes (108).

5. The intelligent tissue slice culture device according to claim 1, wherein: the adjusting mechanism comprises a lifting disc (202) and a first rotating column (205) which are arranged on a first lifting frame (201), a second rotating column (206) is arranged on the first rotating column (205) in a rotating mode, a wiping head (203) is arranged at one end of the second rotating column (206), a limiting ball (207) is arranged at the other end of the second rotating column (206), the limiting ball (207) is connected with the lifting disc (202) in a sliding mode, and the lifting disc (202) can achieve adjustment of the position of the wiping head (203) when sliding on the first lifting frame (201).