CN117463264B

CN117463264B - Automatic reflux device and synthesizer

Info

Publication number: CN117463264B
Application number: CN202311825880.1A
Authority: CN
Inventors: 沈玥; 江湘儿; 黄云; 徐露生; 蔡冬; 张虎; 梁承龙; 刘俞斌
Original assignee: Changzhou New Yichan Life Technology Co ltd; BGI Shenzhen Co Ltd
Current assignee: Changzhou New Yichan Life Technology Co ltd; BGI Shenzhen Co Ltd
Priority date: 2023-12-28
Filing date: 2023-12-28
Publication date: 2024-05-10
Anticipated expiration: 2043-12-28
Also published as: CN117463264A

Abstract

The application discloses an automatic reflux device and a synthesizer, and relates to the technical field of biological macromolecule synthesis. The automatic reflux device comprises a reflux container, a reflux pipe, a reflux bin, a driving piece and a power source. The reflux container is used for collecting the biochip, the reflux bin comprises a main body part and a cover plate, the main body part is provided with an opening, a cavity is arranged in the main body part and communicated with the opening, the reflux container is communicated with the cavity through a reflux pipe, a driving piece is connected with the cover plate and is used for driving the cover plate to cover or expose the opening, and a power source is communicated with the cavity and the reflux container and is used for enabling the biochip in the reflux container to reflux into the cavity. The automatic reflux device and the synthesizer provided by the application can automatically reflux the biochip into the feeding device positioned below the reflux bin, thereby improving the processing efficiency.

Description

Automatic reflux device and synthesizer

Technical Field

The application relates to biological macromolecule synthesis, in particular to an automatic reflux device and a synthesizer.

Background

Common biological macromolecules include proteins, nucleic acids (DNA, RNA, etc.), carbohydrates. Biological macromolecules are mostly polymerized from simple constituent structures, the constituent units of proteins are amino acids, and the constituent units of nucleic acids are nucleotides. The biomacromolecule can be synthesized in vivo by a simple structure. If the biomacromolecule is needed to be synthesized artificially, a special instrument, namely a synthesizer, is needed to synthesize the biomacromolecule.

The existing synthesizer conveys the biochip to a preset position for processing (such as synthesis reaction) through a feeding device, and the biochip which is not conveyed to the preset position or is not processed needs to be collected and conveyed again through the feeding device.

Disclosure of Invention

An object of the present application is to provide an automatic reflow apparatus and synthesizer capable of automatically reflowing a biochip to a feeding device.

The first aspect of the application provides an automatic reflux device comprising a reflux container, a reflux pipe, a reflux bin, a driving piece and a power source. The reflux container is used for collecting the biochip, the reflux bin comprises a main body part and a cover plate, the main body part is provided with an opening, a cavity is arranged in the main body part and communicated with the opening, the reflux container is communicated with the cavity through a reflux pipe, a driving piece is connected with the cover plate and is used for driving the cover plate to cover or expose the opening, and a power source is communicated with the cavity and the reflux container and is used for enabling the biochip in the reflux container to reflux into the cavity.

According to some embodiments of the application, the reflow bin further comprises a guide plate, the guide plate comprises a fixing part and a guide part, the fixing part is fixedly connected with the main body part, the guide part is bent and extended from one end of the fixing part and is arranged at intervals with the main body part, and the cover plate is supported on the guide part and is arranged between the guide part and the main body part in a sliding manner.

According to some embodiments of the application, the reflow bin further comprises a partition plate fixed in the cavity and dividing the cavity into a first cavity and a second cavity isolated from each other, the reflow container comprises a first reflow container and a second reflow container which are arranged at intervals, the first reflow container and the first cavity are positioned on the same side of the automatic reflow device, and the second reflow container and the second cavity are positioned on the other side of the automatic reflow device.

According to some embodiments of the application, the automatic reflow apparatus further includes a connector provided to the main body portion and configured to communicate the cavity with the power source.

According to some embodiments of the application, the cavity comprises a bottom wall and a side wall arranged at the periphery of the bottom wall, the bottom wall and the side wall surround to form the cavity, the main body part comprises a side surface opposite to the side wall, the side surface is provided with a first through hole, the side wall is provided with a plurality of second through holes, a channel is arranged between the side wall and the side surface, the channel is communicated with the first through hole and the plurality of second through holes, and the connector penetrates through the first through hole and is partially accommodated in the channel.

According to some embodiments of the application, the plurality of second through holes are located in the middle of the side wall in a direction from the bottom wall to the opening.

According to some embodiments of the application, the main body comprises a first surface and a second surface which are oppositely arranged, the opening is formed in the second surface, the main body is provided with a connecting hole, the connecting hole penetrates through the first surface and the bottom wall, and one end of the return pipe is inserted into the connecting hole.

According to some embodiments of the application, the body portion includes a first surface and a second surface disposed opposite to each other and a plurality of sides connecting the first surface and the second surface, the opening is disposed on the second surface, the body portion is provided with a notch, the notch penetrates the second surface and communicates with the opening, the notch also penetrates the sides and communicates with the cavity, and the reflow bin further includes a transparent cover plate covering the notch.

According to some embodiments of the application, the automatic reflow apparatus further includes a first mount including a first bottom plate, a first top plate, and a first side plate connecting the first bottom plate and the first top plate, the first bottom plate, the first top plate, and the first side plate enclosing a first through slot having an opening, the reflow container being supported on the first top plate, the first top plate being provided with a through hole through which the reflow tube passes, the reflow tube being partially accommodated in the first through slot.

According to some embodiments of the application, the automatic reflow apparatus further comprises a second mounting seat, the second mounting seat comprises a second bottom plate, a second top plate, a mounting column and a first mounting plate, the mounting column is connected between the second bottom plate and the second top plate, the first mounting plate is sleeved on the mounting column, and the reflow bin is mounted on the first mounting plate.

The second aspect of the application provides a synthesizer comprising a feeding device, an identification device, a control device, a synthesizing device, a sorting device, a transferring device and any one of the automatic reflux devices. The feeding device comprises a conveying component capable of conveying a plurality of biochips simultaneously, the identification device is used for identifying the identification of the biochips on the conveying component and feeding generated identification information back to the control device, the synthesis device comprises a plurality of reaction containers, the reaction containers are used for carrying out synthesis reaction on the identified biochips, the sorting device comprises a plurality of sorting components which are positioned on the side face of the conveying component and are respectively opposite to the plurality of reaction containers, the sorting device is used for sorting the plurality of biochips to the reaction containers corresponding to the current synthesis reaction of the biochips under the control of the control device, the transfer device is used for transferring the biochips on the conveying component to the backflow container, and the backflow bin is arranged above the feeding device.

According to some embodiments of the application, the synthesizer further comprises a sensing device for detecting a spacing between two adjacent biochips on the transfer assembly, a first of the reflow containers being disposed proximate an upstream end of the transfer assembly and for collecting biochips transferred due to the spacing between the two adjacent biochips being less than a predetermined spacing, and a second of the reflow containers being disposed proximate a downstream end of the transfer assembly and for collecting biochips that are not identified by the identifying device or sorted by the sorting device.

The automatic reflux device and the synthesizer provided by the embodiment of the application are provided with the reflux container, the reflux bin and the power source which are sequentially communicated, the biochip collected in the reflux container is refluxed into the reflux bin by using the power source, and the cover plate of the reflux bin is driven to be opened by using the driving piece, so that the biochip is automatically refluxed into the feeding device positioned below the reflux bin, and the processing efficiency is improved.

Drawings

The foregoing and/or additional aspects and advantages of the application will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:

fig. 1 is a schematic perspective view of an automatic reflow apparatus according to an embodiment of the application.

Fig. 2 is a schematic perspective view of a reflow chamber and a driving member of the automatic reflow apparatus shown in fig. 1.

Fig. 3 is a schematic perspective view of the reflow oven and the drive member of fig. 2 from another perspective.

Fig. 4 is an exploded view of the main body portion of the reflow cartridge of fig. 2.

Fig. 5 is a perspective cutaway view of the body portion of the cartridge of fig. 2.

Description of the main reference signs

An automatic reflow apparatus 100;

A reflow container 10;

A reflow bin 20;

A driving member 30;

a power source 40;

A first mount 50;

a second mount 60;

A return pipe 70;

A main body 21;

A cover plate 22;

A cavity 210;

a first surface 21a;

a second surface 21b;

A side surface 21c;

A connection hole 21a1;

An opening 21b1;

A joint 24;

A guide plate 26;

a fixed portion 261;

a guide portion 262;

a connection block 27;

A cylinder 31;

A piston rod 32;

A first joint 33;

a second joint 34;

a first bottom plate 51;

a first top plate 52;

a first side plate 53;

A first through groove 50a;

a second bottom plate 61;

a second top plate 62;

A second side plate 63;

a mounting post 64;

a first mounting plate 65;

A second mounting plate 66;

a second through groove 60a;

a partition plate 23;

A first cavity 210a;

a second cavity 210b;

A first reflow container 11;

a second reflow container 12;

A bottom wall 21d;

a side wall 21e;

a first through hole 21c1;

A second through hole 21e1;

A notch 216;

a transparent cover plate 25;

A valve assembly 80;

A valve seat 81;

A solenoid valve 82;

A first vent valve 83;

A second vent valve 84.

Detailed Description

The following description of the embodiments of the present application will clearly and specifically describe the technical solutions of the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

In addition, the dimensions or thicknesses of various components, layers may be exaggerated in the drawings for brevity and clarity. Like numbers refer to like elements throughout. As used herein, the term "and/or," "and/or" includes any and all combinations of one or more of the associated listed items. In addition, it should be understood that when element a is referred to as "connecting" element B, element a may be directly connected to element B, or intermediate element C may be present and element a and element B may be indirectly connected to each other.

Further, the use of "may" when describing embodiments of the present application refers to "one or more embodiments of the present application".

The terminology used herein is for the purpose of describing particular embodiments and is not intended to be limiting of the application. As used herein, the singular is intended to include the plural as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, values, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, values, steps, operations, elements, components, and/or groups thereof.

It will be understood that, although the terms first, second, third, etc. may be used herein to describe various elements, components, regions, layers and/or sections, these elements, components, regions, layers and/or sections should not be limited by these terms. These terms are used to distinguish one element, component, region, layer or section from another element, component, region, layer or section. Thus, a first element, component, region, layer or section discussed below could be termed a second element, component, region, layer or section without departing from the teachings of the example embodiments.

Referring to fig. 1, an automatic reflow apparatus 100 according to an embodiment of the present application includes a reflow container 10, a reflow chamber 20, a driving member 30, and a power source 40. The reflow container 10 is used for collecting biochips (not shown) to be transferred again by a feeding device (not shown). The return cartridge 20 communicates with the return vessel 10 via a return pipe 70 for dispensing biochips to the supply device. The driving member 30 is used to drive the reflow bin 20 to open or close. The power source 40 communicates with the reflow chamber 20 and the reflow container 10 for reflowing the biochip in the reflow container 10 into the reflow chamber 20 when the reflow chamber 20 is in a closed state.

Referring to fig. 1 to 3, the reflow chamber 20 includes a main body 21 and a cover 22. A cavity 210 is provided in the body 21 for accommodating a biochip. The main body portion 21 includes a first surface 21a and a second surface 21b that are disposed opposite to each other, and a plurality of side surfaces 21c that connect the first surface 21a and the second surface 21 b. The first surface 21a, the second surface 21b, and the side surface 21c are exposed outside the main body 21. The first surface 21a is provided with a connection hole 21a1, and one end of the return pipe 70 is inserted into the connection hole 21a1 to communicate with the cavity 210. The return pipe 70 and the connection hole 21a1 may be connected by interference fit or screw connection. The second surface 21b is provided with an opening 21b1, the opening 21b1 communicating with the cavity 210. The cover plate 22 serves to close the opening 21b1 and to support the biochip received in the cavity 210. The driving member 30 is coupled to the cover 22 and serves to drive the cover 22 to move to close or expose the opening 21b1.

In the present embodiment, the power source 40 is a vacuum generator. The side 21c is provided with a nipple 24, the nipple 24 being in communication with the cavity 210. The fitting 24 is also in communication with the power source 40 via an air line (not shown) such that the power source 40 is in communication with the cavity 210 and the return vessel 10. In the case where the cover 22 closes the opening 21b1, the power source 40 is activated to generate negative pressure in the cavity 210, and the biochip in the reflow container 10 is introduced into the cavity 210 through the reflow tube 70 by the negative pressure. When the biochip enters the cavity 210, the driving member 30 drives the cover 22 to move and expose the opening 21b1, so that the biochip received in the cavity 210 and supported by the cover 22 can be directly dropped into the feeding device located below the reflow bin 20, and automatic reflow of the biochip transferred from a downstream device (e.g., synthesizing device, etc.) of the feeding device to the feeding device is achieved. In other embodiments, the power source 40 is a vacuum pump or other power device capable of moving the biochip in the reflow container 10 through the reflow tube 70 into the cavity 210.

The reflow bin 20 also includes a deflector 26. The guide plate 26 is fixedly arranged on the side surface 21c and is used for guiding the cover plate 22, so that the cover plate 22 can move relative to the main body 21 conveniently. The guide plate 26 is substantially L-shaped and includes a fixed portion 261 fixedly connected to the main body portion 21 and a guide portion 262 bent from one end of the fixed portion 261 and provided at a distance from the main body portion 21. The cover 22 is supported by the guide portion 262 and slidably disposed between the guide portion 262 and the main body portion 21. The size of the gap between the guide portion 262 and the main body portion 21 and the thickness of the cover plate 22 are adapted so that the cover plate 22 can abut between the guide portion 262 and the main body portion 21 and slide relative to the cover plate 22 and the guide portion 262. In the present embodiment, the number of the guide plates 26 is two, and the two guide plates 26 are fixedly provided on the two opposite side surfaces 21c. The cover plate 22 is guided by two guide plates 26 positioned at both sides of the cover plate 22, so that the guide stability is improved.

The driving member 30 is fixedly connected with the cover plate 22 through the connection block 27. In the present embodiment, the driving tool 30 is a cylinder. Specifically, the driving member 30 includes a cylinder 31, a piston rod 32, a first joint 33, and a second joint 34. When fed through the first joint 33, it is discharged through the second joint 34; conversely, when air is fed in through the second joint 34, air is discharged through the first joint 33. One end of the piston rod 32 is movably disposed in the cylinder 31, and the other end thereof extends out of the cylinder 31. The piston rod 32 may be driven to extend or retract the cylinder 31 by controlling the first and second joints 33 and 34 to respectively take in and out, or to respectively take in and out. The connection block 27 is connected to a piston rod 32. When the piston rod 32 is extended or retracted, the cover plate 22 moves away from or near the cylinder 31 with the piston rod 32, so that the cover plate 22 closes or reveals the opening 21b1. In other embodiments, the driver 30 is a motor.

Referring to fig. 1, the automatic reflow apparatus 100 further includes a first mount 50 for supporting the reflow container 10. The first mount 50 includes a first bottom plate 51, a first top plate 52, and a first side plate 53 connecting the first bottom plate 51 and the first top plate 52. The first bottom plate 51, the first top plate 52, and the first side plate 53 enclose the first through groove 50a having an opening. The first base plate 51 may be mounted to the ground. The reflow container 10 is supported on the first top plate 52. The first top plate 52 is provided with a through hole (not shown) communicating with the first through groove 50a for passing the return pipe 70 therethrough to connect with the return tank 10. The return pipe 70 passing through the through-hole is partially accommodated in the first through-groove 50a, and one end of the return pipe 70 protrudes out of the first mount 50 through the opening of the first through-groove 50a to be connected with the return bin 20. In the present embodiment, the first mounting base 50 includes two first side plates 53 disposed opposite to each other, and the first bottom plate 51, the first top plate 52, and the two first side plates 53 enclose a first through groove 50a formed with openings at both ends. In other embodiments, the number of first side plates 53 may be three or the like.

Referring to fig. 1, the automatic reflow apparatus 100 further includes a second mount 60 for supporting the reflow bin 20. The second mount 60 includes a second bottom plate 61, a second top plate 62, mounting posts 64, and a first mounting plate 65. The second base plate 61 may be mounted to the ground. The mounting post 64 is connected between the second bottom plate 61 and the second top plate 62. The first mounting plate 65 is sleeved on the mounting post 64. The main body 21 of the reflow chamber 20 is mounted on the first mounting plate 65. In the present embodiment, the second top plate 62 is closer to the first mounting plate 65 than the second bottom plate 61, i.e. the first mounting plate 65 is disposed at the upper portion of the second mounting seat 60, so that the reflow bin 20 mounted on the first mounting plate 65 and the ground can have enough space to facilitate the arrangement of the feeding device below the reflow bin 20.

Referring to fig. 1, the second mounting base 60 further includes three second side plates 63, and the second bottom plate 61, the second top plate 62, and the three second side plates 63 enclose a second through slot 60a having an opening. The mounting post 64 is accommodated in the second through groove 60a, and the first mounting plate 65 is sleeved on the mounting post 64 and extends out of the second through groove 60a.

Referring to fig. 1, the second mounting base 60 further includes a second mounting plate 66, and the second mounting plate 66 is used for mounting the driving member 30. Specifically, the second mounting plate 66 is connected to the main body 21, and the driver 30 is mounted to the second mounting plate 66. The second mounting plate 66 is closer to the second base plate 61 than the first mounting plate 65 to facilitate coupling the cover plate 22 with the driver 30.

In some embodiments, the first mounting plate 65 is slidably received over the mounting posts 64 to facilitate adjustment of the height of the reflow bin 20 relative to the ground.

Referring to fig. 1 to 3, the reflow bin 20 further includes a partition 23, where the partition 23 is fixed in the cavity 210 and divides the cavity 210 into two cavities, a first cavity 210a and a second cavity 210b, which are isolated from each other. The first and second chambers 210a and 210b are respectively communicated with the two power sources 40 to independently control the first and second chambers 210a and 210b to generate power. The first surface 21a is provided with two connection holes 21a1, wherein one connection hole 21a1 is communicated with the first cavity 210a, and the other connection hole 21a1 is communicated with the second cavity 210b. The number of the reflow containers 10 is two, namely a first reflow container 11 and a second reflow container 12, the first reflow container 11 and the second reflow container 12 are arranged at intervals, the first reflow container 11 and the first cavity 210a are positioned on the same side of the automatic reflow device 100, and the second reflow container 12 and the second cavity 210b are positioned on the other side of the automatic reflow device 100. Specifically, for convenience of piping connection, the first return tank 11 may communicate with the second chamber 210b, and the second return tank 12 may communicate with the first chamber 210 a. In the present embodiment, the first reflux container 11 and the second reflux container 12 are both funnels. The number of the first mounting seats 50 is two, and the first reflow container 11 and the second reflow container 12 are respectively mounted on the two first mounting seats 50. The first reflow container 11 and the first cavity 210a are located at the same side of the automatic reflow apparatus 100, and the second reflow container 12 and the second cavity 210b are located at the other side of the automatic reflow apparatus 100. The first return tank 11 communicates with the second chamber 210b through the corresponding return pipe 70 and the connection hole 21a1, and the second return tank 12 communicates with the first chamber 210a through the corresponding return pipe 70 and the connection hole 21a 1. In other embodiments, the partition 23 may not be included, i.e., there is only one cavity inside the body portion 21; or the number of the partitions 23 is plural and divides the cavity 210 into a plurality of cavities isolated from each other. It will be appreciated that the number of power sources 40 and the number of return vessels 10 are equal to the number of cavities within the body portion 21.

Referring to fig. 2 and 5, the cavity 210 includes a bottom wall 21d and a plurality of side walls 21e disposed around the periphery of the bottom wall 21d, and the bottom wall 21d and the plurality of side walls 21e enclose the cavity 210. The side wall 21e is opposite to the side face 21 c. The connection hole 21a1 penetrates the first surface 21a and the bottom wall 21d. The edge of the side wall 21e remote from the bottom wall 21d surrounds the opening 21b1. The side surface 21c is provided with a first through hole 21c1, and the side wall 21e is provided with a plurality of second through holes 21e1. The first through hole 21c1 and the plurality of second through holes 21e1 are located opposite to each other. A channel (not shown) is provided between the side wall 21e and the side 21c for receiving the connector 24. The connector 24 passes through the first through hole 21c1 and is fixedly connected in the passage to communicate with the cavity 210. The dimensions of the first through hole 21c1 and the dimensions of the channel are adapted to the dimensions of the joint 24. The joint 24 may be connected to the first through hole 21c1 and the inside of the passage by screwing. The second through hole 21e1 is smaller in size than the tab 24 such that the tab 24 cannot extend through the second through hole 21e1 into the cavity 210. The second through hole 21e1 has a smaller size than the biochip, and prevents the biochip from entering the channel through the second through hole 21e1. The biochip may be in a regular shape such as rectangle, square, trapezoid, etc., or in an irregular shape such as ellipse. The diameter of the second through hole 21e1 is smaller than the minimum length of the biochip in the length direction and the minimum width in the width direction. In one embodiment, the diameter of the second through hole 21e1 is smaller than half of the smaller one of the minimum length and the minimum width of the biochip. For example, the square shape of the biochip, the length of which is 2mm and the width of which is 2mm, and the diameter of the second through-hole 21e1 may be 0.9mm, 0.8mm, 0.7mm, 0.6mm, 0.5mm, the risk of the second through-hole 21e1 being blocked by the biochip accommodated in the cavity 210 may be reduced. In fig. 5, the opposite side surfaces 21c are provided with first through holes 21c1, and the opposite side walls 21e are provided with second through holes 21e1, so that the two connectors 24 are respectively communicated with the first cavity 210a and the second cavity 210b through the corresponding first through holes 21c1 and the second through holes 21e1.

In some embodiments, the plurality of second through holes 21e1 are located in the middle of the side wall 21e in the direction from the bottom wall 21d to the opening 21b 1. In the present application, the middle portion of the side wall 21e has a portion overlapping the center of the side wall 21e in the direction from the bottom wall 21d to the opening 21b 1. Alternatively, the ratio of the length of the middle portion of the side wall 21e to the length of the side wall 21e in the direction from the bottom wall 21d to the opening 21b1 is 1/6, 1/5, 1/4 or 1/3. The second through-hole 21e1 is provided adjacent to the center of the sidewall 21e, and the risk of the biochip being electrostatically attracted to the bottom wall 21d during reflow to the cavity 210 can be reduced.

In some embodiments, the body portion 21 has a transparent portion (not shown) to facilitate a user's viewing of the biochip within the cavity 210. Referring to fig. 4, the main body 21 is provided with a notch 216, the notch 216 penetrates through the second surface 21b and communicates with the opening 21b1, and the notch 216 penetrates through the side surface 21c and the side wall 21e and communicates with the cavity 210. The reflow bin 20 also includes a transparent cover plate 25, the transparent cover plate 25 covering the indentations 216. Through the transparent cover plate 25, a user can observe the biochip inside the cavity 210. In fig. 4, the notch 216 is L-shaped, penetrating the adjacent three sides 21c and the opposite two side walls 21e.

In some embodiments, the return tube 70 is made of a transparent material. By configuring the return tube 70 transparent, the biochip inside the tube can be observed.

Referring to fig. 1, the automatic reflow apparatus 100 further includes a valve assembly 80 disposed on the second mounting seat 60. The valve assembly 80 includes a valve seat 81 mounted to the second side plate 63 and located outside the second vent groove 60a, and a solenoid valve 82, a first vent valve 83, and a second vent valve 84 mounted to the valve seat 81. Specifically, in some embodiments, power source 40 is a vacuum generator and solenoid valve 82 is used to control the inlet or outlet of first/second connectors 33, 34 and thus the extension or retraction of piston rod 32 from cylinder 31. The first ventilation valve 83 is used to control on/off of an air source (not shown) of the power source 40 communicating with the first chamber 210a, and negative pressure is generated when ventilation is performed, and negative pressure is not generated when ventilation is performed. The second ventilation valve 84 is used for controlling on/off of a gas source (not shown) of the power source 40 communicated with the second cavity 210 b.

An embodiment of the present application further provides a synthesizer, including a feeding device, an identification device, a control device, a synthesizing device, a sorting device, a transferring device, and an automatic reflow device 100. The feeding device includes a transfer assembly that can simultaneously transfer a plurality of biochips. The identification device is used for identifying the identification of the biochip on the transmission assembly and feeding back the generated identification information to the control device, and the control device confirms the synthesis reaction which the biochip should currently perform according to the identification information. The synthesis device includes a plurality of reaction vessels for performing a synthesis reaction on the biochip. The sorting device comprises a plurality of sorting assemblies which are positioned on the side face of the conveying assembly and are respectively opposite to the plurality of reaction vessels, and the sorting assemblies are used for sorting the identified plurality of biochips positioned on the conveying assembly to the reaction vessels corresponding to the current synthesis reaction of the biochips under the control of the control device. The transfer device is used to transfer the biochip on the transfer assembly to the reflow container 10. The reflow bin 20 is disposed above the feeding device, so that when the cover plate 22 exposes the opening 21b1, the biochip in the reflow bin 20 can automatically drop onto the feeding device under the action of gravity. In this embodiment, the transfer device includes an air supply device for blowing the biochip on the transfer assembly into the reflow container 10 by blowing. In other embodiments, the transfer device includes a robot that transfers the biochip on the transfer assembly to the reflow container 10 by way of a robot grasp.

In some embodiments, the synthesizer further comprises a sensing device. The sensing device is used for detecting the interval between two adjacent biochips on the conveying assembly and feeding back the generated detection information to the control device. When the interval between the adjacent two biochips is smaller than the preset interval, the control means controls the transfer means to transfer one of the adjacent two biochips, which is close to the upstream side in the conveying direction of the conveying assembly, into the reflow container 10.

In some embodiments, the first reflow container 11 is disposed proximate the upstream end of the transfer assembly and the second reflow container 12 is disposed proximate the downstream end of the transfer assembly. In the present application, the upstream end of the transfer member refers to one end of the transfer member located on the upstream side in the transfer direction, and the downstream end of the transfer member refers to the other end of the transfer member located on the downstream side in the transfer direction. The first reflow container 11 serves to collect the biochips transferred on the transfer assembly due to the interval between the adjacent biochips being smaller than the preset interval. The second reflow container 12 is used to collect the biochips on the transfer assembly that are not recognized by the recognition means or sorted to the synthesis means by the sorting means. In this embodiment, the biochips transferred because the pitch between adjacent biochips is smaller than the preset pitch are transferred to the first reflow container 11 by the transfer device, and the biochips on the transfer assembly that are not recognized by the recognition device or sorted to the synthesizing device by the sorting device drop directly into the second reflow container 12 at the downstream end of the transfer assembly. In other embodiments, biochips on the transfer assembly that are not identified by the identification means or that are not sorted to the synthesis means by the sorting means are also transferred to the second reflow container 12 by the transfer means.

The foregoing disclosure is merely illustrative of the present application and is not intended to limit the application thereto, therefore, equivalent variations of the application may be made and fall within the scope of the application.

Claims

1. The automatic reflux device is characterized by comprising a reflux container, a reflux pipe, a reflux bin, a driving piece and a power source, wherein the reflux container is used for collecting biological chips, the reflux bin comprises a main body part and a cover plate, the main body part is provided with an opening, a cavity is formed in the main body part and communicated with the opening, the reflux container is communicated with the cavity through the reflux pipe, the driving piece is connected with the cover plate and used for driving the cover plate to cover or expose the opening, the power source is communicated with the cavity and the reflux container and used for enabling the biological chips in the reflux container to reflux into the cavity, the automatic reflux device further comprises a connector, the cavity comprises a bottom wall and a side wall arranged at the periphery of the bottom wall, the bottom wall and the side wall surround the cavity, the side wall is far away from the edge of the bottom wall and surrounds the opening, the side surface opposite to the side wall is provided with a first through hole, the side wall is provided with a plurality of second through holes, the side wall is provided with a plurality of through holes, the side wall and the side wall is provided with a plurality of channels and the first through holes and the power source are communicated with the first through holes and the power source. The backflow bin further comprises a guide plate and a baffle plate, the guide plate comprises a fixing part and a guide part, the fixing part is fixedly connected with the main body part, the guide part is bent and extended from one end of the fixing part and is arranged with the main body part at intervals, the cover plate is supported on the guide part and is arranged between the guide part and the main body part in a sliding manner, the baffle plate is fixed in the cavity and divides the cavity into a first cavity and a second cavity which are isolated from each other, and the backflow container comprises a first backflow container and a second backflow container which are arranged at intervals.

2. The automatic reflow apparatus of claim 1, wherein the first reflow container and the first cavity are located on the same side of the automatic reflow apparatus, and the second reflow container and the second cavity are located on the other side of the automatic reflow apparatus.

3. The automatic reflow apparatus of claim 1, wherein the plurality of second through holes are located in a middle portion of the side wall in a direction from the bottom wall to the opening.

4. The automatic reflow apparatus of claim 1, wherein the main body includes a first surface and a second surface disposed opposite to each other, the opening is formed in the second surface, the main body has a connection hole, the connection hole penetrates through the first surface and the bottom wall, and one end of the reflow tube is inserted into the connection hole.

5. The automatic reflow apparatus of claim 1, wherein the body includes a first surface and a second surface disposed opposite each other and a plurality of sides connecting the first surface and the second surface, the opening is formed in the second surface, the body is provided with a notch extending through the second surface and communicating with the opening, the notch also extends through the sides and communicating with the cavity, and the reflow chamber further includes a transparent cover plate covering the notch.

6. The automatic reflow apparatus of claim 1, further comprising a first mount including a first bottom plate, a first top plate, and a first side plate connecting the first bottom plate and the first top plate, the first bottom plate, the first top plate, and the first side plate enclosing a first channel having an opening, the reflow container supported on the first top plate, the first top plate having a through hole through which the reflow tube passes, the reflow tube being partially received in the first channel.

7. The automatic reflow apparatus of claim 1, further comprising a second mount including a second bottom plate, a second top plate, a mounting post, and a first mounting plate, the mounting post connected between the second bottom plate and the second top plate, the first mounting plate sleeved on the mounting post, the reflow bin mounted on the first mounting plate.

8. A synthesizer comprising a feeding device, an identification device for identifying the identification of a biochip on the delivery device and feeding back the generated identification information to the control device, a control device for performing a synthesis reaction on the identified biochip, a synthesizing device for sorting the biochip to a reaction container corresponding to the current synthesis reaction of the biochip under the control of the control device, a sorting device for sorting the biochip to a reaction container corresponding to the current synthesis reaction of the biochip, the sorting device being disposed above the feeding device, and an automatic reflow device according to any one of claims 1 to 7.

9. The synthesizer of claim 8, further comprising a sensing device for detecting a spacing between adjacent two of the biochips on the conveyor assembly, a first one of the reflow containers being disposed proximate an upstream end of the conveyor assembly and for collecting biochips diverted due to a spacing between adjacent two of the biochips being less than a predetermined spacing, and a second one of the reflow containers being disposed proximate a downstream end of the conveyor assembly and for collecting biochips not identified by the identification device or sorted by the sorting device.