CN117448405A - Method for producing donkey-hide gelatin peptide - Google Patents
Method for producing donkey-hide gelatin peptide Download PDFInfo
- Publication number
- CN117448405A CN117448405A CN202311184210.6A CN202311184210A CN117448405A CN 117448405 A CN117448405 A CN 117448405A CN 202311184210 A CN202311184210 A CN 202311184210A CN 117448405 A CN117448405 A CN 117448405A
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- Prior art keywords
- donkey
- hide gelatin
- enzymolysis
- peptide
- water
- Prior art date
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- Pending
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- 108010010803 Gelatin Proteins 0.000 title claims abstract description 68
- 239000008273 gelatin Substances 0.000 title claims abstract description 68
- 229920000159 gelatin Polymers 0.000 title claims abstract description 68
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 68
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 68
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 66
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 108010019160 Pancreatin Proteins 0.000 claims abstract description 15
- 229940055695 pancreatin Drugs 0.000 claims abstract description 15
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 14
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 14
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 14
- 108010007119 flavourzyme Proteins 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 239000002245 particle Substances 0.000 claims abstract description 10
- 239000011259 mixed solution Substances 0.000 claims abstract description 9
- 230000001105 regulatory effect Effects 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 3
- 239000000047 product Substances 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 238000001694 spray drying Methods 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 6
- 241000283074 Equus asinus Species 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 102000035195 Peptidases Human genes 0.000 claims description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 claims description 2
- 239000000413 hydrolysate Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 108010038807 Oligopeptides Proteins 0.000 abstract description 9
- 102000015636 Oligopeptides Human genes 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 238000000746 purification Methods 0.000 abstract description 2
- 108010052008 colla corii asini Proteins 0.000 description 12
- 229910052799 carbon Inorganic materials 0.000 description 6
- 230000006837 decompression Effects 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 229920001542 oligosaccharide Polymers 0.000 description 3
- 150000002482 oligosaccharides Chemical class 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 239000004365 Protease Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Abstract
The invention provides a production method of donkey-hide gelatin peptide, which comprises the following steps: mixing donkey-hide gelatin powder with water, and regulating the pH value to 6.5-7 to obtain a mixed solution, wherein the weight-to-volume (g/L) ratio of the donkey-hide gelatin powder to the water is 150-200:1, and the particle size of the donkey-hide gelatin powder is 60 meshes; (2) enzymolysis: adding 7-8 u/g neutral protease, 3-4 u/g pancreatin and 5-5.5 u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 40-60 ℃ and the enzymolysis time is 3-5 hours, so as to obtain enzymolysis liquid; (3) purification: and decoloring and drying the enzymolysis liquid to obtain a product. The production method has the advantages of simple and easily controlled process and strong stability. The donkey-hide gelatin peptide obtained by the method has the oligopeptide content (on a dry basis) of more than 90 percent; the content of small molecular peptide with molecular weight less than or equal to 3000Da is more than 96 percent; the donkey-hide gelatin peptide obtained by the method has high content of small molecular peptide.
Description
Technical Field
The invention relates to the technical field of donkey-hide gelatin peptide production, in particular to a production method of donkey-hide gelatin peptide.
Background
Decocting donkey hide, concentrating to obtain solid gel, namely donkey-hide gelatin. The main component of the donkey-hide gelatin is protein, has good nourishing performance, and is one of the products to be favored along with the improvement of the health consciousness of people.
Donkey-hide gelatin is mainly macromolecular protein, and has certain difficulty in digestion and absorption after eating, so that the donkey-hide gelatin is treated by an enzymolysis method commonly used at present to obtain small molecular peptide; because the small molecular peptide can be directly absorbed and utilized by human body to improve the immunity of the human body, the research on the enzymolysis of donkey-hide gelatin to obtain the small molecular peptide is gradually increasing.
Although the research on preparing the small molecular peptide by the enzymolysis of donkey-hide gelatin is more, the hydrolysis degree of macromolecular protein is lower in the prior art, and the occupation of the molecular weight of the obtained small molecular peptide less than 3000Da is lower, so that the hydrolysis effect of the prior art is poor.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a production method of donkey-hide gelatin peptide, which comprises the steps of material preparation, enzymolysis and purification. The donkey-hide gelatin peptide obtained by the method has the oligopeptide content (calculated on a dry basis) more than 90%; the content of small molecular peptide with molecular weight less than or equal to 3000Da is more than 96 percent; the donkey-hide gelatin peptide obtained by the method has high content of small molecular peptide. The production method has the advantages of simple and easily controlled process and strong stability.
The technical scheme of the invention is as follows:
a method for producing donkey-hide gelatin peptide comprises the following steps:
(1) Preparing materials: mixing donkey-hide gelatin powder with water, and regulating the pH value to 6.5-7 to obtain a mixed solution, wherein the weight-to-volume (g/L) ratio of the donkey-hide gelatin powder to the water is 150-200:1, and the particle size of the donkey-hide gelatin powder is 60 meshes;
(2) Enzymolysis: adding 7-8 u/g neutral protease, 3-4 u/g pancreatin and 5-5.5 u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 40-60 ℃ and the enzymolysis time is 3-5 hours, so as to obtain enzymolysis liquid;
(3) Purifying: and decoloring and drying the enzymolysis liquid to obtain a product.
Preferably, in the step (1), the weight-to-volume (g/L) ratio of the donkey-hide gelatin powder to water is 170:1000.
Preferably, in step (2), 7.5u/g of neutral protease, 3.5u/g of pancreatin and 5.5u/g of flavourzyme are added to the mixture.
Preferably, in the step (2), the enzymolysis temperature is 45 ℃ and the enzymolysis time is 4 hours.
Preferably, in the step (3), activated carbon is added into the enzymatic hydrolysate, and the enzymatic hydrolysate is decolorized to obtain a removal solution.
Preferably, in step (3), the removal solution is concentrated and then spray dried.
Preferably, the spray drying conditions are an inlet temperature of 175 ℃ and an outlet temperature of 80 ℃.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, through setting the particles of the donkey-hide gelatin powder, the situation that water-in-water powder is generated when the donkey-hide gelatin powder is mixed with water is avoided, the mixing degree of water and the donkey-hide gelatin powder is improved, the subsequent enzymolysis process is facilitated, and the enzymolysis efficiency is improved.
2. According to the invention, neutral protease, pancreatin and flavor protease are used in combination, so that on one hand, the content of micromolecular peptide in the product can be effectively increased, and on the other hand, the smell and taste of the product can be improved.
3. The donkey-hide gelatin peptide obtained by the method has the oligopeptide content (calculated on a dry basis) of more than 90 percent; the content of small molecular peptide with molecular weight less than or equal to 3000Da is more than 96 percent; the donkey-hide gelatin peptide obtained by the method has high content of small molecular peptide.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the description of the embodiments or the prior art will be briefly described below, and it will be obvious to those skilled in the art that other drawings can be obtained from these drawings without inventive effort.
FIG. 1 is a graph showing the molecular weight distribution of donkey-hide gelatin peptide of example 1.
FIG. 2 shows donkey-hide gelatin peptide test report-1 of example 1.
FIG. 3 shows donkey-hide gelatin peptide test report-2 of example 1.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
Example 1
A method for producing donkey-hide gelatin peptide comprises the following steps:
(1) Preparing materials: mixing colla Corii Asini powder with water, and regulating pH to 6.7 to obtain mixed solution, wherein the weight volume (g/L) ratio of colla Corii Asini powder and water is 170:1000, and the particle size of colla Corii Asini powder is 60 mesh;
(2) Enzymolysis: adding 7.5u/g neutral protease, 3.5u/g pancreatin and 5.5u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 45 ℃ and the enzymolysis time is 4 hours, so as to obtain enzymolysis liquid;
(3) Purifying: adding active carbon into the enzymolysis liquid for decoloring and deoiling, and collecting a removal liquid; concentrating the removed liquid by using a decompression concentrating device, then spray drying at the inlet temperature of 175 ℃ and the outlet temperature of 80 ℃, and crushing to obtain the product.
The physicochemical detection and molecular weight distribution detection of the product are carried out, and the results are shown in figures 1-3.
Referring to FIGS. 1 to 3, the oligopeptide content (on a dry basis) of the donkey-hide gelatin peptide is 90.7%; the content of the small molecular peptide with the molecular weight less than or equal to 3000Da is 96.24 percent.
Example 2
A method for producing donkey-hide gelatin peptide comprises the following steps:
(1) Preparing materials: mixing colla Corii Asini powder with water, and regulating pH to 6.5 to obtain mixed solution, wherein the weight volume (g/L) ratio of colla Corii Asini powder and water is 150:1, and the particle size of colla Corii Asini powder is 60 mesh;
(2) Enzymolysis: adding 7u/g neutral protease, 3u/g pancreatin and 5u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 50 ℃ and the enzymolysis time is 5 hours, so as to obtain enzymolysis liquid;
(3) Purifying: adding active carbon into the enzymolysis liquid for decoloring and deoiling, and collecting a removal liquid; concentrating the removed liquid by using a decompression concentrating device, then spray drying at the inlet temperature of 175 ℃ and the outlet temperature of 80 ℃, and crushing to obtain the product.
In the donkey-hide gelatin peptide, the oligosaccharide content (based on dry basis) is 90.4%; the content of small molecular peptide with molecular weight less than or equal to 3000Da is 96.17 percent.
Example 3
A method for producing donkey-hide gelatin peptide comprises the following steps:
(1) Preparing materials: mixing donkey-hide gelatin powder with water, and regulating pH to 7 to obtain a mixed solution, wherein the weight-to-volume (g/L) ratio of the donkey-hide gelatin powder to the water is 200:1, and the particle size of the donkey-hide gelatin powder is 60 meshes;
(2) Enzymolysis: adding 8u/g neutral protease, 4u/g pancreatin and 5.5u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 50 ℃ and the enzymolysis time is 4 hours, so as to obtain an enzymolysis liquid;
(3) Purifying: adding active carbon into the enzymolysis liquid for decoloring and deoiling, and collecting a removal liquid; concentrating the removed liquid by using a decompression concentrating device, then spray drying at the inlet temperature of 175 ℃ and the outlet temperature of 80 ℃, and crushing to obtain the product.
In the donkey-hide gelatin peptide, the content of oligopeptide (on a dry basis) is 90.1 percent; the content of small molecular peptide with molecular weight less than or equal to 3000Da is 96.22 percent.
Example 4
A method for producing donkey-hide gelatin peptide comprises the following steps:
(1) Preparing materials: mixing colla Corii Asini powder with water, and regulating pH to 6.6 to obtain mixed solution, wherein the weight volume (g/L) ratio of colla Corii Asini powder and water is 160:1, and the particle size of colla Corii Asini powder is 60 mesh;
(2) Enzymolysis: adding 7.7u/g neutral protease, 3.3u/g pancreatin and 5.5u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 55 ℃ and the enzymolysis time is 4.5 hours, so as to obtain an enzymolysis liquid;
(3) Purifying: adding active carbon into the enzymolysis liquid for decoloring and deoiling, and collecting a removal liquid; concentrating the removed liquid by using a decompression concentrating device, then spray drying at the inlet temperature of 175 ℃ and the outlet temperature of 80 ℃, and crushing to obtain the product.
In the donkey-hide gelatin peptide, the content of oligopeptide (on a dry basis) is 90.3%; the content of small molecular peptide with molecular weight less than or equal to 3000Da is 96.19 percent.
Example 5
A method for producing donkey-hide gelatin peptide comprises the following steps:
(1) Preparing materials: mixing colla Corii Asini powder with water, and regulating pH to 6.6 to obtain mixed solution, wherein the weight volume (g/L) ratio of colla Corii Asini powder and water is 150:1, and the particle size of colla Corii Asini powder is 60 mesh;
(2) Enzymolysis: adding 8u/g neutral protease, 3u/g pancreatin and 5u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 56 ℃ and the enzymolysis time is 5 hours, so as to obtain enzymolysis liquid;
(3) Purifying: adding active carbon into the enzymolysis liquid for decoloring and deoiling, and collecting a removal liquid; concentrating the removed liquid by using a decompression concentrating device, then spray drying at the inlet temperature of 175 ℃ and the outlet temperature of 80 ℃, and crushing to obtain the product.
In the donkey-hide gelatin peptide, the content of oligosaccharide (based on dry basis) is 90.2%; the content of small molecular peptide with molecular weight less than or equal to 3000Da is 96.05 percent.
Example 6
A method for producing donkey-hide gelatin peptide comprises the following steps:
(1) Preparing materials: mixing donkey-hide gelatin powder with water, and regulating pH to 7.0 to obtain mixed solution, wherein the weight-to-volume (g/L) ratio of the donkey-hide gelatin powder to the water is 190:1, and the particle size of the donkey-hide gelatin powder is 60 meshes;
(2) Enzymolysis: adding 7.3u/g neutral protease, 3.5u/g pancreatin and 5.5u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 58 ℃ and the enzymolysis time is 5 hours, so as to obtain enzymolysis liquid;
(3) Purifying: adding active carbon into the enzymolysis liquid for decoloring and deoiling, and collecting a removal liquid; concentrating the removed liquid by using a decompression concentrating device, then spray drying at the inlet temperature of 175 ℃ and the outlet temperature of 80 ℃, and crushing to obtain the product.
In the donkey-hide gelatin peptide, the oligosaccharide content (based on dry basis) is 90.6%; the content of small molecular peptide with molecular weight less than or equal to 3000Da is 96.22 percent.
As can be seen from the results of examples 1 to 6, the method of the present invention has the advantage of high donkey-hide gelatin peptide content. The content of small molecular peptide with molecular weight less than or equal to 3000Da in the donkey-hide gelatin peptide prepared by the method of the invention is more than 96%.
Comparative example 1
The difference from example 1 is that no neutral protease was added, but only pancreatin was added at 7.5u/g, flavourzyme 9u/g; in the donkey-hide gelatin peptide, the content of oligopeptide (on a dry basis) is 70.4%; the content of the small molecular peptide with the molecular weight less than or equal to 3000Da is 55.43 percent.
Comparative example 2
The difference from example 1 is that no pancreatin was added, but only 9.5u/g neutral protease and 7u/g flavourzyme were added; in the donkey-hide gelatin peptide, the content of oligopeptide (on a dry basis) is 67.1 percent; the content of the small molecular peptide with the molecular weight less than or equal to 3000Da is 52.76 percent.
Comparative example 3
The difference from example 1 is that no flavourzyme is added, but only 8.5u/g neutral protease and 8u/g pancreatin are added; in the donkey-hide gelatin peptide, the content of oligopeptide (on a dry basis) is 74.9%; the content of the small molecular peptide with the molecular weight less than or equal to 3000Da is 62.08 percent.
Compared with the prior art, the three enzymes added in the donkey-hide gelatin peptide have a synergistic effect, so that the content of small molecular peptide (molecular peptide is less than or equal to 3000 Da) in the donkey-hide gelatin peptide is high through the synergistic effect of the three enzymes. In the absence of either enzyme, the effect of the present invention could not be achieved.
Although the present invention has been described in detail by way of reference to preferred embodiments, the present invention is not limited thereto. Various equivalent modifications and substitutions may be made in the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and it is intended that all such modifications and substitutions be within the scope of the present invention/be within the scope of the present invention as defined by the appended claims. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
1. A method for producing donkey-hide gelatin peptide is characterized by comprising the following steps:
(1) Preparing materials: mixing donkey-hide gelatin powder with water, and regulating the pH value to 6.5-7 to obtain a mixed solution, wherein the weight-to-volume (g/L) ratio of the donkey-hide gelatin powder to the water is 150-200:1, and the particle size of the donkey-hide gelatin powder is 60 meshes;
(2) Enzymolysis: adding 7-8 u/g neutral protease, 3-4 u/g pancreatin and 5-5.5 u/g flavourzyme into the mixed liquor; the enzymolysis temperature is 40-60 ℃ and the enzymolysis time is 3-5 hours, so as to obtain enzymolysis liquid;
(3) Purifying: and decoloring and drying the enzymolysis liquid to obtain a product.
2. The method of producing donkey hide gelatin peptide according to claim 1, wherein in step (1), the weight-to-volume (g/L) ratio of donkey hide gelatin powder to water is 170:1000.
3. The method for producing donkey-hide gelatin peptide according to claim 1, wherein in step (2), 7.5u/g of a proteolytic enzyme, 3.5u/g of pancreatin and 5.5u/g of a flavourzyme are added to the mixture.
4. The method for producing donkey-hide gelatin peptide according to claim 1, wherein in the step (2), the enzymolysis temperature is 45 ℃ and the enzymolysis time is 4 hours.
5. The method for producing donkey-hide gelatin peptide according to claim 1, wherein in the step (3), activated carbon is added to the enzymatic hydrolysate, and the enzymatic hydrolysate is decolorized to obtain a removal solution.
6. The method for producing donkey-hide gelatin peptide according to claim 1, wherein in step (3), the removal liquid is concentrated and then spray-dried.
7. The method for producing donkey hide gelatin peptide according to claim 6, wherein the spray drying conditions are inlet temperature 175 ℃ and outlet temperature 80 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311184210.6A CN117448405A (en) | 2023-09-14 | 2023-09-14 | Method for producing donkey-hide gelatin peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311184210.6A CN117448405A (en) | 2023-09-14 | 2023-09-14 | Method for producing donkey-hide gelatin peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117448405A true CN117448405A (en) | 2024-01-26 |
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ID=89584356
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311184210.6A Pending CN117448405A (en) | 2023-09-14 | 2023-09-14 | Method for producing donkey-hide gelatin peptide |
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| Country | Link |
|---|---|
| CN (1) | CN117448405A (en) |
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2023
- 2023-09-14 CN CN202311184210.6A patent/CN117448405A/en active Pending
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