CN117418003A - 标志物、探针及其应用 - Google Patents
标志物、探针及其应用 Download PDFInfo
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- CN117418003A CN117418003A CN202210851062.8A CN202210851062A CN117418003A CN 117418003 A CN117418003 A CN 117418003A CN 202210851062 A CN202210851062 A CN 202210851062A CN 117418003 A CN117418003 A CN 117418003A
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- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000013441 quality evaluation Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C40B40/04—Libraries containing only organic compounds
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- General Chemical & Material Sciences (AREA)
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Abstract
Description
反应物 | 1次反应体积 |
Ligation Buffer | 23μl |
T4 DNA Ligase | 2μl |
合计 | 25μl |
步骤 | 温度 | 时间 |
步骤1 | 20℃ | 15分钟 |
步骤2 | 72℃ | 15分钟 |
步骤3 | 4℃ | Hold |
反应物 | 1次反应体积 |
End Repair-A Tailing Buffer | 16μl |
End Repair-A Tailing Enzyme Mix | 4μl |
合计 | 20μl |
步骤 | 温度 | 时间 |
步骤1 | 20℃ | 30分钟 |
步骤2 | 4℃ | 保持 |
反应物 | 1次反应体积 |
5x HerculaseⅡBuffer with dNTPs | 10μl |
HerculaseⅡFusion DNA Polymerase | 1μl |
合计 | 11μl |
DNA投入量 | 循环数 |
100-200ng | 8 |
10-100ng | 9 |
<10ng | 11 |
反应物 | 单个反应体积 |
SureSelect RNase Block | 0.5ul |
Nuclease-free water | 1.5ul |
Total | 2ul |
反应物 | 单个反应体积 |
25%RNase Block solution(from step 9.4) | 2ul |
Probe(with design<3MB) | 2ul |
SureSelect Fast Hybridization Buffer | 6ul |
Nuclease-free water | 3ul |
Total | 13ul |
Claims (14)
Priority Applications (1)
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CN202210851062.8A CN117418003A (zh) | 2022-07-19 | 2022-07-19 | 标志物、探针及其应用 |
Applications Claiming Priority (1)
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CN202210851062.8A CN117418003A (zh) | 2022-07-19 | 2022-07-19 | 标志物、探针及其应用 |
Publications (1)
Publication Number | Publication Date |
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CN117418003A true CN117418003A (zh) | 2024-01-19 |
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Application Number | Title | Priority Date | Filing Date |
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CN202210851062.8A Pending CN117418003A (zh) | 2022-07-19 | 2022-07-19 | 标志物、探针及其应用 |
Country Status (1)
Country | Link |
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CN (1) | CN117418003A (zh) |
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2022
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