CN117417870A - Bacillus sphaericus, composition and application thereof in saline-alkali soil improvement - Google Patents

Bacillus sphaericus, composition and application thereof in saline-alkali soil improvement Download PDF

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CN117417870A
CN117417870A CN202311746076.4A CN202311746076A CN117417870A CN 117417870 A CN117417870 A CN 117417870A CN 202311746076 A CN202311746076 A CN 202311746076A CN 117417870 A CN117417870 A CN 117417870A
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saline
salt
soil
alkali soil
sphaericus
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CN117417870B (en
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刘方春
马海林
杨庆山
刘幸红
刘丙花
潘博雅
憨化景
孙铭婕
彭琳
杜振宇
马丙尧
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Shandong Academy of Forestry
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Shandong Academy of Forestry
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00—Methods for working soil
    • A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C09K2101/00—Agricultural use
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    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

Abstract

The invention belongs to the technical field of microorganism and soil treatment and restoration, and particularly relates to bacillus sphaericus, a composition and application thereof in saline-alkali soil improvement. Specifically, the bacillus sphaericus is successfully screened to obtain the bacillus sphaericus, salt leaching of saline-alkali soil can be effectively accelerated, meanwhile, the bacillus sphaericus is matched with Pityrosporum indicum for use, the salt content of the saline-alkali soil can be obviously reduced, and the soil desalination rate is improved. In a word, according to the characteristics of different microorganisms, the invention reduces the content of water-soluble salt in soil from the two angles of accelerating salt leaching and reducing salt returning, comprehensively improves the saline-alkali soil, has simple operation, low price and environmental friendliness, and therefore has good practical application value.

Description

Bacillus sphaericus, composition and application thereof in saline-alkali soil improvement
Technical Field
The invention belongs to the technical field of microorganism and soil treatment and restoration, and particularly relates to bacillus sphaericus, a composition and application thereof in saline-alkali soil improvement.
Background
With the increasing global warming problem, the problem of soil salinization has become a global concern for various countries, about 7% of the land in the world is threatened by salinization, and this figure is rising. China is a country with serious soil salinization, the salinized soil has large area and wide distribution, ecological environment in the area is fragile, ecological problems are prominent, and the regional economy development is seriously restricted by the soil salinization.
The ecological management of saline-alkali soil is a long-term and complex system engineering, relates to multiple disciplines and has long-term complexity. The research of the degenerated ecological system aiming at the improvement and utilization of the saline-alkali soil is a worldwide major scientific and technical problem, and a plurality of scholars and institutions at home and abroad are engaged in related researches. At present, the development and utilization methods of the saline-alkali soil at home and abroad mainly comprise a hydraulic engineering method, a physical method, a chemical method and a biological method. The biggest problem faced by saline-alkali soil improvement is that the salt leaching difficulty is high, salt is easy to return in arid seasons, and the salt surface aggregation phenomenon is serious.
Patent 202010125947.0 discloses a method for promoting saline-alkali soil salt leaching, which promotes saline-alkali soil salt leaching by bacillus mucilaginosus, garden waste compost, bentonite and other materials, improves soil fertility and soil physical structure, is favorable for irrigating water and washing salt, and inhibits surface salt returning. The patent 202210904213.1 discloses a method for avoiding salt returning in spring of saline-alkali cultivated lands, which is characterized in that crop straws are left for 20 cm-30 cm, simultaneously, the straw harvester is used for crushing the straws while harvesting seeds, or the straw harvester is used for crushing the harvested crop straws, and the crushed straws are uniformly sprayed and covered on the surface of the cultivated lands, so that moisture can quickly permeate into the soil of the saline-alkali cultivated lands, evaporation of the water on the surface is reduced, saline-alkali ions are prevented from rising to the surface along with the evaporation of the soil, the saline-alkali ions are detained in the deep layer of the soil, and the salt returning of the soil is effectively prevented. However, the inventor discovers that the method has the defects of complicated preparation and treatment process, poor treatment effect and the like, and influences the large-scale popularization and use of the method.
Disclosure of Invention
In view of the above, the invention aims to provide a bacillus sphaericus strain which can effectively accelerate salt leaching of saline-alkali soil.
It is another object of the present invention to provide a composition comprising B.sphaericus and Pityrosporum indicum, which can significantly reduce the salt content of the soil in saline-alkali soil and increase the desalination rate of the soil.
It is another object of the present invention to provide the related application of the above-mentioned lysine bacillus sphaericus, composition in soil improvement of saline-alkali soil.
In order to achieve the above purpose, the present invention provides the following technical solutions:
lysine bacillus sphaericus strainLysinibacillus sphaericus) 6', the biological preservation number is CGMCC No. 26114, and the strain is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) on 11 th year 2022.
The bacillus sphaericus 6' is separated from a tamarix rhizosphere soil sample in a germplasm resource library of yellow river delta saline-alkali tolerant trees in a Tianning temple forest farm in a reclamation area of eastern camping city, and is gram positive bacteria, gelatin liquefaction positive, starch hydrolysis experiment positive, nitrate reduction experiment positive, phenylalanine dehydrogenase experiment positive, contact enzyme experiment positive, indole experiment positive and V-P experiment negative. The 16S rDNA sequence is shown as SEQ ID NO. 1.
In a specific embodiment of the present invention, there is provided a microbial agent comprising the above-mentioned lysine bacillus sphaericus 6'.
In a specific embodiment of the present invention, the microbial agent further comprises a carrier in addition to the active ingredient. The carrier may be a carrier commonly used in the field of bacteriological agents and which is biologically inert.
The carrier may be a solid carrier or a liquid carrier;
the solid carrier can be mineral material, plant material or high molecular compound; the mineral material may be at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth; the plant material may be at least one of corn flour, soy flour and starch; the high molecular compound can be polyvinyl alcohol or/and polyglycol;
the liquid carrier may be an organic solvent, vegetable oil, mineral oil, or water; the organic solvent can be decane or/and dodecane.
The dosage form of the microbial inoculum can be various dosage forms, such as liquid, emulsion, suspending agent, powder, granule, wettable powder or water dispersible granule.
Surfactants (such as Tween 20, tween 80, etc.), binders, stabilizers (such as antioxidants), pH regulators, etc. can also be added into the microbial inoculum according to the need.
In a specific embodiment of the present invention, there is provided a composition comprising at least any one of the following (a 1) to (a 2):
(a1) The above-mentioned lysine bacillus sphaericus 6' and mycorrhizal fungi;
(a2) The microbial agents and mycorrhizal fungi.
Wherein the mycorrhizal fungi are preferably fungi with rapid propagation speed and vigorous mycelium growth, such as Boletus fuscosus, agaricus bisporus, pityrosporum indicum, etc. In a specific embodiment of the invention, the mycorrhizal fungus is Pityrosporum indicum.
In a specific embodiment of the present invention, there is provided the above-mentioned lysine bacillus sphaericusLysinibacillus sphaericus) 6', microbial agents and/or compositions in saline-alkali soil improvement.
Specifically, the improvement of the saline-alkali soil is represented by any one or more of the following:
(b1) Accelerating the salt leaching of the saline-alkali soil and inhibiting the salt aggregation;
(b2) Inhibiting soil salt returning;
(b3) Reducing the salt content of the saline-alkali soil;
(b4) The soil desalination rate is improved.
In a specific embodiment of the present invention, there is provided a method for improving saline-alkali soil, the method comprising: applying spherical lysine bacillus to saline-alkali soil environmentLysinibacillus sphaericus) 6', microbial agents and/or compositions.
In a specific embodiment of the invention, the method comprises: the bacillus sphaericus 6' or the microbial agent is applied to saline-alkali soil before planting the spring salt tolerant plants.
In a specific embodiment of the invention, after irrigation (or rainfall) of the saline-alkali soil, planting of salt-tolerant plants is started; and (5) inoculating mycorrhizal fungi after the rainy season is finished.
In a specific embodiment of the present invention, the salt tolerant plants include, but are not limited to, tamarix chinensis, pyrus dorsalis, nitraria tangutica and fraxinus chinensis, and the salt tolerant plants are selected to be symbiotic with the mycorrhizal fungi used, and in a specific embodiment of the present invention, the mycorrhizal fungi are Pityrosporum indicum.
The beneficial technical effects of one or more of the technical schemes are as follows:
(1) The spherical lysine bacillus 6' selected by the technical scheme has strong salt tolerance, has strong extracellular polysaccharide production capacity in a high-salt soil environment, has the functions of enhancing the content of soil water-stable aggregates and recombining soil aggregate structures, and can accelerate the leaching speed of saline-alkali soil.
(2) According to the technical scheme, the characteristic that mycorrhizal fungi and part of salt-tolerant plants can be symbiotic is utilized, the propagation speed of mycorrhizal fungi is improved, the growth speed of mycelia is increased, soil capillary is cut off by utilizing the mycelia of mycorrhizal fungi, and the phenomena of soil salt returning and salt surface aggregation caused by evaporation are inhibited.
(3) According to the technical scheme, according to the characteristics of different microorganisms, the water-soluble salt content of soil is reduced from the two angles of accelerating salt leaching and reducing salt returning, and the saline-alkali soil is comprehensively improved.
Biological preservation description:
bacillus sphaericusLysinibacillus sphaericus) 6', the strain was deposited in China general microbiological culture Collection center (address: the biological preservation number of the Beijing, the Chaoyang district, north Chen Xili No.1, 3, china academy of sciences microbiological study) is CGMCC No. 26114.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention.
FIG. 1 is a diagram of a laboratory experiment for carrying out an indoor soil column simulation test using a PVC pipe in the laboratory stage of example 2 of the present invention.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
As described above, the current methods for developing and utilizing saline-alkali soil at home and abroad mainly comprise hydraulic engineering methods, physical methods, chemical methods and biological methods. The biggest problem faced by saline-alkali soil improvement is that the salt leaching difficulty is high, salt is easy to return in arid seasons, and the salt surface aggregation phenomenon is serious.
Therefore, the invention fully utilizes the characteristics of the bacillus sphaericus 6' such as strong salt tolerance, exopolysaccharide production, soil water stability aggregate enhancement, soil aggregate structure recombination and the like, and combines the characteristics of mycorrhizal fungi (such as Pityrosporum indicum) capable of cutting off soil capillary and effectively inhibiting salt return, so that the salt leaching of saline-alkali soil is accelerated, the salt return of the soil is inhibited, and the invention has great significance for the improvement of the saline-alkali soil.
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention.
Example 1
Bacillus sphaericusLysinibacillus sphaericus) 6', the steps of the screening method are as follows:
separating bacteria from a tamarix chinensis rhizosphere soil sample in a saline-alkali-tolerant tree germplasm resource library of yellow river delta in a Tianning temple forest farm in a reclamation area of eastern camping, purifying the separated bacteria by a three-area scribing method, numbering the purified bacteria, and preserving on an LB inclined plane for later use. And (3) performing primary screening by a wheat leaf green-keeping method, performing secondary screening by increasing the weight of radish cotyledons to obtain candidate strains, and finally determining the extracellular polysaccharide production capacity of the desired candidate strains to obtain the lysine bacillus 6'.
The 16s rDNA sequence (SEQ ID NO. 1) was entered into the GenBank database website of the NCBI website for similarity comparison. The strain is identified and named as the bacillus sphaericus by combining physiological biochemistry, colony morphology and indexes (the bacillus sphaericus 6' is gram positive bacterium, gelatin liquefaction positive, starch hydrolysis positive, nitrate reduction positive, phenylalanine dehydrogenase positive, contact enzyme positive, indole positive and V-P negative)Lysinibacillus sphaericus）6’。
Example 2: leaching experiments
Adopting PVC pipe (diameter 7.5cm, height 40 cm) to carry out indoor soil column simulation test, 6 treatments are provided for the test, which are (1) CK: adding water; (2) inoculating lysine bacillus sphaericus 6'; (3) inoculating bacillus subtilis GE3; (4) inoculating bacillus aryabhattai BPR078; (5) inoculating bacillus fusiformis L13; (6) Inoculating Pityrosporum indicumSerendipita indica）。
Bacillus subtilis GE3, bacillus aryabhattai BPR078, bacillus fusiformis L13 and Bacillus sphaericus 6' were inoculated into LB medium, respectively, cultured under shaking at 28℃and 180 rpm for 24 h, and the bacterial suspension was adjusted with sterile physiological saline (2.0X10) 8 cfu/mL) were made into GE3, BPR078, L13 and Bacillus sphaericus 6' inoculants, respectively.
Pityrosporum indicum stock was inoculated onto PDA solid medium (pH=6.5), incubated under dark conditions at constant temperature and humidity (25.+ -. 1 ℃, 60.00%) for 1 week, and subjected to plate activation and proliferation culture. Punching at the edge of activated colony with sterile puncher with diameter of 5 mm, collecting mycelium blocks, placing in Pityrosporum indicum liquid medium, placing in rotary incubator, culturing in dark for 3 days (32 deg.C, 60.00% RH,180 rpm), and concocting to spore content of 10 5 Inoculant per mL.
The Pityrosporum indicum liquid medium comprises the following formula: potato filtrate 100mL (water 100mL, potato 20 g), wort 4.370 mL, yeast powder 0.052 g, magnesium sulfate 0.040 g.
The Pityrosporum indicum (CGMCC 3.17686) is purchased from China general microbiological culture Collection center (China Committee for culture Collection).
The soil used in the experiment is taken from a germplasm resource library of yellow river delta saline-alkali tolerant tree in Tianning temple forest farm in the reclamation area of eastern camping, and the original salt content is 8.2%. After passing the soil through a2 mm sieve, 100ml inoculants (4.46% according to the different experimental designv/ w) Mixing with 2.24kg soil, stirring, and stirring to 1.69. 1.69 g/cm 3 Column loading was performed uniformly and each treatment was repeated 7 times.
The bottom cover of the PVC pipe is uniformly perforated and covered with 4 layers of permeable cloth to prevent soil leakage, the soil column is erected in a leaching liquid collecting cylinder after filling, a layer of filter paper is placed on the surface of the soil layer to prevent soil from being scattered when water is injected, 100ml of deionized water is poured into the soil layer, and the soil layer is stood for 10 d.10 And d, beginning to inject water into the soil column for leaching, wherein the water injection amount is 420ml, recording the leaching time, and measuring the conductivity of the leaching solution. It can be seen that the treatment of the bacillus sphaericus 6' obviously reduces the leaching time, and the conductivity of the leaching liquid is obviously higher than that of other treatments; the Pilospora indica treatment obviously increases the leaching time, and the conductivity of the leaching liquid is obviously reduced. The other microbial agents have little influence on the salt leaching speed and the conductivity of the leaching solution. This demonstrates that lysine bacillus sphaericus 6' can significantly promote salt leaching in soil, while piriform Pityrosporum indicum has a certain inhibition effect on salt leaching.
Table 1 results of indoor soil column simulation test under different treatment conditions
Example 3: field experiments
The field experiment is arranged in a germplasm resource library of yellow river delta saline-alkali tolerant tree in Tianning temple forest farm in the eastern camping and reclamation area, the experiment starts at 2022 and 4 months, and four treatments are respectively: (1) control: no inoculant is added; (2) inoculating lysine bacillus sphaericus 6'; (3) inoculating Pityrosporum indicum (Si); (4) The seed was inoculated with B.sphaericus 6 'followed by Pityrosporum indicum (6' +Si). The preparation of lysine bacillus sphaericus 6' and pyriform indicum inoculants was performed as in example 2, liquid fermentation culture.
In 2022, 4 months, treatment 2 and treatment 4 cells used bacillus sphaericus 6' with irrigation to saline-alkali soil in an amount of 15 liters per mu, and with irrigation measures, the microbial inoculant was applied to saline-alkali soil. The planting tree is Lu Cheng first (1 year container seedling), and 400 tamarix chinensis are planted in 4 processing cells respectively. And after the 8 months rainy season is finished, inoculating Pityrosporum indicum in the areas of treatment 3 and treatment 4, and when the Pityrosporum indicum is inoculated with mycorrhizal fungi, punching 3 holes around the planted salt-tolerant plants, and inoculating Pityrosporum indicum inoculant around the root systems of the salt-tolerant plants, wherein the inoculation amount of each plant of salt-tolerant forest is 30ml. The salt content and the desalination rate of the surface soil (0 to 20 cm) in the different cells were measured at the beginning of 11 months, and the results are shown in Table 2 below. It can be seen that the combination application of the bacillus sphaericus 6' and the Pityrosporum indicum can obviously reduce the salt content of the saline-alkali soil and improve the soil desalination rate.
TABLE 2 results of salt content in surface soil
16S rDNA sequence of B.sphaericus 6':
AGCCGGGGCGGGCGTGCTATACATGCAAGTCGAGCGAACAGAGAAGGAGCTTGCTCCTTTGACGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTACCCTATAGTTTGGGATAACTCCGGGAAACCGGGGCTAATACCGAATAATCTTTTGTCCCTCATGGGACAATACTGAAAGACGGTTTCGGCTGTCGCTATAGGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGATTTCGGTTCGTAAAACTCTGTTGTAAGGGAAGAACAAGTACAGTAGTAACTGGCTGTACCTTGACGGTACCTTATTAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGATAGTGGAATTCCAAGTGTAGCGGTGAAATGCGTAGAGATTTGGAGGAACACCAGTGGCGAAGGCGACTATCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCGTTGACCACTGTAGAGATATGGTTTTCCCTTCGGGGACAACGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCATCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGATACAAACGGTTGCCAACTCGCGAGAGGGAGCTAATCCGATAAAGTCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGAATAGAGG（SEQ ID NO.1）。
finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. Lysine bacillus sphaericus strainLysinibacillus sphaericus) 6', wherein the strain is preserved in China general microbiological culture collection center (CGMCC) at 11/2022, and the biological preservation number is CGMCC No. 26114.
2. A microbial agent comprising at least the spherical lysine according to claim 1Bacillus [ ]Lysinibacillus sphaericus）6’。
3. A composition, characterized in that it comprises at least any one of the following (a 1) to (a 2):
(a1) The bacillus sphaericus of claim 1Lysinibacillus sphaericus) 6' and mycorrhizal fungi;
(a2) The microbial agent and mycorrhizal fungi of claim 2.
4. A composition according to claim 3, wherein the mycorrhizal fungi are selected from bolete fusca, agaricus bisporus and pyriform indicum.
5. The bacillus sphaericus of claim 1Lysinibacillus sphaericus) Use of a microbial agent according to claim 2 and/or a composition according to any one of claims 3 to 4 for saline-alkali soil improvement.
6. The use of claim 5, wherein the saline-alkali soil improvement is manifested as any one or more of the following:
(b1) Accelerating the salt leaching of the saline-alkali soil and inhibiting the salt aggregation;
(b2) Inhibiting soil salt returning;
(b3) Reducing the salt content of the saline-alkali soil;
(b4) The soil desalination rate is improved.
7. A method for improving saline-alkali soil, the method comprising: applying the bacillus sphaericus of claim 1 to a saline-alkali soil environmentLysinibacillus sphaericus) 6', the microbial agent of claim 2 and/or the composition of any one of claims 3-4.
8. The method of claim 7, wherein the method comprises: planting of salt-tolerant plants in springBefore, the bacillus sphaericus is treatedLysinibacillus sphaericus) 6' or the microbial agent is applied to the saline-alkali soil.
9. The method of claim 8, further comprising, after irrigation of the saline-alkali soil or rainfall, starting to plant the salt-tolerant plant; and (5) inoculating mycorrhizal fungi after the rainy season is finished.
10. The method of claim 9, wherein the salt tolerant plant comprises tamarix chinensis, pyrus chinensis, nitraria tangutica and fraxinus chinensis, and the mycorrhizal fungus is pyriform indicum.
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CN111100816A (en) * 2020-01-09 2020-05-05 根力多生物科技股份有限公司 Spherical lysine bacillus SY50 and application thereof

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KR20150111528A (en) * 2014-03-25 2015-10-06 인하대학교 산학협력단 Lysinibacillus sphaericus and composition containing the same
CN104838842A (en) * 2015-04-20 2015-08-19 天津师范大学 Method adopting salt-tolerant reinforcement nanometer garbage compost for regulating turf grass protective enzyme under salt stress
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