CN117417814B - Full-automatic virus extraction system and extraction method - Google Patents
Full-automatic virus extraction system and extraction method Download PDFInfo
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- CN117417814B CN117417814B CN202311738521.2A CN202311738521A CN117417814B CN 117417814 B CN117417814 B CN 117417814B CN 202311738521 A CN202311738521 A CN 202311738521A CN 117417814 B CN117417814 B CN 117417814B
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- 241000700605 Viruses Species 0.000 title claims abstract description 112
- 238000000605 extraction Methods 0.000 title claims abstract description 105
- 239000007788 liquid Substances 0.000 claims abstract description 132
- 238000000967 suction filtration Methods 0.000 claims abstract description 65
- 238000002360 preparation method Methods 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000000463 material Substances 0.000 claims abstract description 22
- 239000002699 waste material Substances 0.000 claims abstract description 21
- 238000003860 storage Methods 0.000 claims abstract description 20
- 230000010355 oscillation Effects 0.000 claims abstract description 17
- 239000000243 solution Substances 0.000 claims description 22
- 230000007246 mechanism Effects 0.000 claims description 20
- 238000002386 leaching Methods 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 13
- 239000012086 standard solution Substances 0.000 claims description 12
- 238000009210 therapy by ultrasound Methods 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 10
- 239000013049 sediment Substances 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- 238000005303 weighing Methods 0.000 claims description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 5
- 229940098773 bovine serum albumin Drugs 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 238000002604 ultrasonography Methods 0.000 claims description 5
- 239000007853 buffer solution Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000009295 crossflow filtration Methods 0.000 claims description 4
- 230000003993 interaction Effects 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 238000011065 in-situ storage Methods 0.000 claims description 2
- 210000001503 joint Anatomy 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 abstract description 11
- 238000000034 method Methods 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 10
- 238000011112 process operation Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000003612 virological effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000001360 synchronised effect Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
Abstract
The invention discloses a full-automatic virus extraction system and an extraction method. The full-automatic virus extraction system comprises a liquid preparation container, a standard liquid container, a water storage container, a centrifugal container, an ultrasonic centrifugal oscillating multifunctional integrated machine, a suction filtration device, vacuum equipment, a suction filtration container, a tangential flow device, a material receiving container and a waste container. The full-automatic virus extraction system can realize three-in-one innovation of liquid oscillation, centrifugation and ultrasonic in the centrifugal container, improves the virus extraction experimental efficiency, performs full-process automatic operation on virus extraction, performs high automation on manual and trivial experimental steps of operators in the traditional technology, has more convenient process operation, reduces manual extraction errors, and ensures the virus extraction effect and virus activity to the greatest extent.
Description
Technical Field
The application relates to the technical fields of biotechnology and medical equipment, in particular to a full-automatic virus extraction system and an extraction method thereof.
Background
At present, the occurrence of viruses in the environment and the ecological functions thereof are widely concerned, but the current knowledge of the composition and the functions of the viruses is still insufficient, which is closely related to the complex pretreatment flow. At present, the extraction of virus liquid aiming at an environmental medium mainly needs to be performed by a plurality of flows such as extraction, ultrasonic treatment, centrifugation, filtration and the like, but a virus enrichment instrument-tangential flow system on the market mainly aims at concentration of terminal viruses, so that development of a full-automatic virus liquid extraction system from extraction to concentration and preservation is needed.
Disclosure of Invention
Based on this, it is necessary to provide a fully automated virus extraction system. The full-automatic virus extraction system can realize three-in-one innovation of oscillation, centrifugation and ultrasonic, improves the virus extraction experimental efficiency, automatically extracts viruses in a full process, highly automates manual trivial experimental steps, has more convenient process operation, reduces the extraction error, and ensures the virus extraction effect and activity to the greatest extent.
An embodiment of the present application provides a fully automated virus extraction system.
A full-automatic virus extraction system comprises a liquid preparation container, a standard liquid container, a water storage container, a centrifugal container, an ultrasonic centrifugal oscillating multifunctional integrated machine, a suction filtration device, vacuum equipment, a suction filtration container, a tangential flow device, a material receiving container and a waste container; the liquid preparation container is detachably connected to the centrifugal container through a pipeline, the standard liquid container is connected to the centrifugal container so as to be used for adding standard liquid into the centrifugal container, the centrifugal container is arranged in the ultrasonic centrifugal oscillating multifunctional integrated machine and is in balancing arrangement with a balancing piece, the ultrasonic centrifugal oscillating multifunctional integrated machine can realize ultrasonic, centrifugal and oscillating functions in situ, the centrifugal container is connected to the suction filtration device, the suction filtration device is connected to the suction filtration container, the suction filtration container is connected to the tangential flow device, the suction filtration container is connected to the vacuum equipment, the tangential flow device is connected to the material receiving container and the waste container, and the water storage container is connected to the ultrasonic centrifugal oscillating multifunctional integrated machine so as to be used for adding water into the ultrasonic centrifugal oscillating multifunctional integrated machine for ultrasound preparation.
In some embodiments, the dispensing container comprises a first dispensing barrel, a second dispensing barrel; the centrifugal container comprises a first centrifugal bottle and a second centrifugal bottle; the suction filtration device comprises a first suction filtration machine and a second suction filtration machine; the suction filtration container comprises a first suction filtration barrel and a second suction filtration barrel; the material receiving container comprises a first material receiving barrel and a second material receiving barrel;
the first liquid preparation barrel is detachably connected to the first centrifugal bottle through a first pipeline, the second liquid preparation barrel is detachably connected to the second centrifugal bottle through a second pipeline, the standard liquid container is simultaneously connected to the first centrifugal bottle and the second centrifugal bottle for enabling standard liquid to be added to the first centrifugal bottle and the second centrifugal bottle respectively, the first centrifugal bottle and the second centrifugal bottle are both arranged in the ultrasonic centrifugal oscillating multifunctional integrated machine, the first centrifugal bottle is connected to the first suction filter, the second centrifugal bottle is connected to the second suction filter, the first suction filter is connected to the first suction filter barrel, the second suction filter is connected to the second suction filter barrel, the first suction filter barrel and the second suction filter barrel are respectively connected to the tangential flow devices, the first suction filter and the second suction filter are respectively connected to the vacuum equipment, and the two tangential flow devices are respectively connected to the first suction filter and the second suction filter.
In some embodiments, the number of the first liquid preparing barrels and the number of the second liquid preparing barrels may be multiple, correspondingly, the number of the first centrifugal bottles, the number of the first suction filters, the number of the first suction filter barrels and the number of the first material collecting barrels are all consistent with the number of the first liquid preparing barrels, the number of the second centrifugal bottles, the number of the second suction filters, the number of the second suction filter barrels and the number of the second material collecting barrels are all consistent with the number of the second liquid preparing barrels, and the number of the tangential flow devices is the sum of the number of the second suction filters and the number of the second material collecting barrels.
In some embodiments, the fully automatic virus extraction system further comprises a first liquid adding pump and a second liquid adding pump;
the first liquid preparation barrel is connected to the first centrifugal bottle through the first liquid adding pump, and the second liquid preparation barrel is connected to the second centrifugal bottle through the second liquid adding pump.
In some embodiments, the vacuum apparatus comprises a first vacuum pump, a second vacuum pump, the first suction filter being connected to the first vacuum pump, the second suction filter being connected to the second vacuum pump.
In some embodiments, the fully automated virus extraction system further comprises a third liquid feed pump; the standard solution container is connected to the centrifugal container through the third liquid adding pump for adding standard solution to the centrifugal container.
In some embodiments, the fully automated virus extraction system further comprises a fourth liquid feed pump; the centrifugal container is connected with the suction filtration device through the fourth liquid adding pump.
In some embodiments, the fully automated virus extraction system further comprises a sixth liquid feed pump; the water storage container is connected to the ultrasonic centrifugal oscillation multifunctional integrated machine through the sixth liquid adding pump and is used for adding water into the ultrasonic centrifugal oscillation multifunctional integrated machine for preparing ultrasonic waves.
In some embodiments, the ultrasonic centrifugal oscillation multifunctional integrated machine comprises an ultrasonic barrel, an ultrasonic vibrator, an oscillator and a centrifuge; the centrifugal containers or the centrifugal containers and the balancing piece are symmetrically arranged in the ultrasonic barrel body through balancing, and the ultrasonic vibrators are arranged in the ultrasonic barrel body to carry out ultrasonic treatment on liquid in the centrifugal containers in the ultrasonic barrel body;
the centrifugal machine is arranged on the centrifugal base and is used for centrifuging the centrifugal container in the ultrasonic barrel body;
the oscillator is connected to the centrifugal base for oscillating the centrifugal container.
In some embodiments, the fully automated virus extraction system further comprises a refrigerator for providing a temperature control function for the fully automated virus extraction system.
In some embodiments, the fully automated virus extraction system further comprises a lifting mechanism connected to the pipeline for driving the pipeline to move up and down to achieve docking and undocking with the centrifuge vessel.
An embodiment of the application also provides a full-automatic virus extraction method.
A full-automatic virus extraction method adopts the full-automatic virus extraction system, and comprises the following steps:
(1) Weighing; weighing a virus sample sediment in a centrifugal container;
(2) Adding the leaching solution into the centrifugal container in the step (1), and shaking uniformly; the leaching solution is prepared by dissolving 1-2wt% of bovine serum albumin in 0.01M PBS buffer solution; the mass ratio of the added virus sample sediment to the leaching solution is 1:3-1:10;
(3) Standing in darkness; controlling a standard liquid container to add an equal volume of standard liquid into a centrifugal container, balancing the centrifugal container in an ultrasonic centrifugal oscillating multifunctional integrated machine, and standing in a dark environment for at least 15 min;
(4) Ultrasonic treatment; controlling the water storage container to add water into the ultrasonic centrifugal vibration multifunctional integrated machine, and controlling the ultrasonic centrifugal vibration multifunctional integrated machine to carry out ultrasonic treatment for at least 15 min at the temperature of 40-50W and the temperature of 4-8 ℃;
(5) Oscillating; controlling the ultrasonic centrifugal oscillating multifunctional integrated machine to oscillate at least 1 h at the rotating speed of 250 rpm-300 rpm;
(6) Centrifuging; controlling the ultrasonic centrifugal oscillating multifunctional integrated machine to centrifuge for at least 10 min at 8000 rpm;
(7) Suction filtration; collecting supernatant liquid in the centrifuged container, pumping the supernatant liquid to a suction filtration device, controlling vacuum equipment to work, enabling the supernatant liquid to sequentially pass through a filter membrane of the suction filtration device, removing large particles and microorganisms, collecting filtrate, and then entering the suction filtration container;
(8) Tangential flow filtration;
1) Cleaning the tangential flow device;
2) Sample adding; and opening the suction filtration container, opening an upper permeation pipeline valve and a reflux valve of the tangential flow device connected with the suction filtration container, allowing filtrate in the suction filtration container to enter the tangential flow device, filtering concentrated virus suspension at a flow rate of 20% -40%, allowing the concentrated solution to enter a corresponding receiving container, and allowing waste liquid in the tangential flow device to enter a waste liquid container.
The full-automatic virus extraction system can realize the innovation of three-in-one of liquid oscillation, centrifugation and ultrasonic of the centrifugal container, improves the experimental efficiency of virus extraction, and performs full-process automatic operation on virus extraction, so that manual and trivial experimental steps of operators in the traditional technology are highly automated, the process operation is more convenient, the manual extraction error is reduced, and the virus extraction effect and the virus activity are ensured to the greatest extent. Specifically, the full-automatic virus extraction system realizes full-automatic procedures from dosing, extraction, vibration, centrifugation, filtration, enrichment and concentration to back flushing, greatly reduces manual participation of operators, liberates manual operation of operators on one hand, improves accuracy and uniformity of virus liquid extraction on the other hand, can realize low-temperature storage of samples, avoids influence of excessive environmental temperature on virus extraction effect, and can more accurately reflect the composition characteristics of viruses in actual environments.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained from these drawings without inventive effort to a person skilled in the art.
For a more complete understanding of the present application and the advantages thereof, reference is now made to the following descriptions taken in conjunction with the accompanying drawings. Wherein like reference numerals refer to like parts throughout the following description.
Fig. 1 is a schematic diagram of a fully automatic virus extraction system according to an embodiment of the invention.
Description of the reference numerals
10. A fully automatic virus extraction system; 110. a first liquid preparation barrel; 120. a second liquid preparation barrel; 130. a standard liquid container; 210. a first centrifuge bottle; 220. a second centrifuge bottle; 300. ultrasonic centrifugal oscillation multifunctional integrated machine; 310. an ultrasonic bucket body; 320. a centrifuge; 410. a first suction filter; 420. a second suction filter; 500. a vacuum device; 610. a first suction filtration barrel; 620. a second suction filtration barrel; 700. tangential flow device; 810. a first receiving barrel; 820. a second receiving barrel; 830. a waste container; 910. a first pipeline; 920. a second pipeline; 1010. a first liquid adding pump; 1020. a second liquid adding pump; 1030. a third liquid adding pump; 1040. a fourth liquid adding pump; 1050. a fifth liquid pump; 1100. and (3) a bracket.
Detailed Description
In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
In the description of the present invention, the meaning of a number is one or more, the meaning of a number is two or more, and greater than, less than, exceeding, etc. are understood to exclude the present number, and the meaning of a number is understood to include the present number. The description of the first and second is for the purpose of distinguishing between technical features only and should not be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Embodiments of the present application provide a fully automated virus extraction system 10 to address the current lack of fully automated virus liquid extraction systems from extraction to concentrate storage. The fully automated virus extraction system 10 will be described with reference to the accompanying drawings.
Referring to fig. 1, fig. 1 is a schematic structural diagram of a fully automatic virus extraction system 10 according to an embodiment of the present application. The fully automated virus extraction system 10 of the present application can be used for fully automated virus liquid extraction from extraction to concentrate preservation.
For a clearer description of the structure of the fully automated virus extraction system 10, the fully automated virus extraction system 10 will be described with reference to the accompanying drawings. Referring to fig. 1, fig. 1 is a schematic diagram illustrating a fully automatic virus extraction system 10 according to an embodiment of the disclosure.
The full-automatic virus extraction system 10 comprises a liquid preparation container, a standard liquid container 130, a water storage container, a centrifugal container, an ultrasonic centrifugal oscillating multifunctional integrated machine 300, a suction filtration device, a vacuum device 500, a suction filtration container, a tangential flow device 700, a material receiving container and a waste container 830. Wherein, join in marriage liquid container and pass through the pipeline detachable connection in centrifugal container. The standard solution container 130 is connected to the centrifuge vessel for adding standard solution thereto. The centrifuge vessel is disposed within the ultrasonic centrifugal oscillation multifunctional all-in-one machine 300 and is trim-set with the trim piece. The ultrasonic centrifugal oscillation multifunctional integrated machine 300 can realize ultrasonic, centrifugal and oscillation functions. The centrifugal container is connected with the suction filtration device. The suction filtration device is connected with the suction filtration container. The suction filtration vessel is connected with a tangential flow device 700. The suction filtration vessel is connected with a vacuum device 500. The tangential flow device 700 is connected to a receiving vessel and a waste vessel 830. The water storage container is connected to the ultrasonic centrifugal vibration multifunctional integrated machine 300 and used for adding water into the ultrasonic centrifugal vibration multifunctional integrated machine 300 for ultrasonic treatment.
In some embodiments, referring to fig. 1, the dispensing container includes a first dispensing barrel 110 and a second dispensing barrel 120; the centrifuge vessel comprises a first centrifuge bottle 210, a second centrifuge bottle 220; the suction filtration device comprises a first suction filter 410 and a second suction filter 420; the suction filtration container comprises a first suction filtration bucket 610 and a second suction filtration bucket 620; the receiving container comprises a first receiving bucket 810 and a second receiving bucket 820. Wherein, the first dispensing barrel 110 is detachably connected to the first centrifuge bottle 210 through the first pipeline 910. The second dispensing barrel 120 is detachably connected to the second centrifuge bottle 220 via a second conduit 920. The standard solution container 130 is simultaneously connected to the first and second centrifuge bottles 210 and 220 for adding standard solution to the first and second centrifuge bottles 210 and 220, respectively. The first centrifuge bottle 210 and the second centrifuge bottle 220 are both disposed in the ultrasonic-centrifugal-vibration multifunctional integrated machine 300. The first centrifuge bottle 210 is connected to a first suction filter 410 and the second centrifuge bottle 220 is connected to a second suction filter 420. The first suction filter 410 is connected to a first suction filter basket 610. The second suction filter 420 is connected to a second suction filter basket 620. The tangential flow devices 700 are connected to the first and second suction filters 410 and 420, respectively. The first suction filter 410 and the second suction filter 420 are connected with a vacuum apparatus 500, respectively. The two tangential flow devices 700 are respectively connected to a first receiving tank 810 and a second receiving tank 820. The two tangential flow devices 700 are also connected to a waste container 830.
In some of these embodiments, the vacuum apparatus 500 includes a first vacuum pump, a second vacuum pump. The first suction filter 410 is connected to a first vacuum pump, and the second suction filter 420 is connected to a second vacuum pump. The first and second vacuum pumps are not shown in the drawings, and only the vacuum apparatus 500 is shown as an illustration.
In some embodiments, the number of first dispensing barrels 110, the number of second dispensing barrels 120 may be plural. Correspondingly, the number of the first centrifuge bottles 210, the number of the first suction filters 410, the number of the first suction filter barrels 610, the number of the first receiving barrels 810 and the number of the first vacuum pumps are consistent with the number of the first liquid preparation barrels 110, the number of the second centrifuge bottles 220, the number of the second suction filters 420, the number of the second suction filter barrels 620, the number of the second receiving barrels 820 and the number of the second vacuum pumps are consistent with the number of the second liquid preparation barrels 120, and the number of the tangential flow devices 700 is the sum of the number of the second suction filters 420 and the number of the second receiving barrels 820.
The number of the first liquid dispensing barrels 110 and the number of the second liquid dispensing barrels 120 may be one or more, and the first centrifuge bottle 210 may be in a balancing and symmetrical state in the ultrasonic centrifugal oscillating multifunctional integrated machine 300 to achieve centrifugation. The first liquid preparation barrel 110 and the second liquid preparation barrel 120 can be the same type of virus sample, and can also be virus samples with different components, namely, the synchronous extraction of the same virus sample can be synchronously realized, the synchronous extraction of different virus samples can also be realized, and the final extraction concentrated solution of the virus samples in each liquid preparation barrel can be separately collected in different material collecting barrels.
In some embodiments, referring to fig. 1, the fully automatic virus extraction system 10 further includes a first fluid pump 1010 and a second fluid pump 1020. Each first dispensing bucket 110 is connected to a corresponding first centrifuge bottle 210 by a corresponding first filling pump 1010. Each second dispensing barrel 120 is connected to a corresponding second centrifuge bottle 220 by a corresponding second filling pump 1020.
In some of these embodiments, the fully automated virus extraction system 10 further includes a third charge pump 1030. The standard solution container 130 is simultaneously connected to the first and second centrifuge bottles 210 and 220 through the third filling pump 1030 for adding standard solution to the corresponding first and second centrifuge bottles 210 and 220, respectively.
In some of these embodiments, the fully automated virus extraction system 10 further includes a fourth charge pump 1040. Each first centrifuge bottle 210 is connected to a first suction filter 410 by a fourth liquid pump 1040, respectively.
In some of these embodiments, the fully automated virus extraction system 10 further includes a fifth charge pump 1050. Each second centrifuge bottle 220 is connected to a second suction filter 420 via a fifth fluid pump 1050, respectively.
In some of these embodiments, the fully automated virus extraction system 10 further comprises a sixth liquid feed pump. The water storage container is connected to the ultrasonic centrifugal oscillation multifunctional integrated machine 300 through a sixth liquid adding pump and is used for adding water into the ultrasonic centrifugal oscillation multifunctional integrated machine 300 for preparing ultrasonic waves. The water storage container and the sixth liquid adding pump are not shown in the drawings.
In some embodiments, when the number of the first suction filters 410 and the number of the second suction filters 420 are respectively plural, the first vacuum pumps are respectively connected to the first suction filters 410. A second vacuum pump is connected to each second suction filter 420.
In some of these embodiments, the ultrasonic centrifugal oscillating multifunctional integrated machine 300 includes an ultrasonic bucket 310, an ultrasonic vibrator, an oscillator, and a centrifuge 320. Wherein the first centrifuge bottle 210 and the second centrifuge bottle 220 are symmetrically disposed in the ultrasonic bucket body 310 by balancing. The ultrasonic vibrator is disposed in the ultrasonic bucket body 310 to ultrasonically treat the liquid in the first centrifuge bottle 210 and the second centrifuge bottle 220 in the ultrasonic bucket body 310. The centrifuge 320 is mounted to the centrifuge base for centrifuging the ultrasonic bucket 310. The oscillator is connected to the centrifugal base for oscillating the centrifugal base and the ultrasonic bucket body 310 connected thereto. The ultrasound vibrator, the oscillator are not shown in the figures.
In some of these embodiments, each of the first and second suction filters 410 and 420 has a 2 μm and 0.22 μm filter membrane, respectively.
In some of these embodiments, the fully automated virus extraction system 10 further comprises a refrigerator. The refrigerator is used to provide a temperature control function for the fully automated virus extraction system 10. The refrigerator provides the whole process temperature condition of 4-8 ℃ for the full-automatic virus extraction method.
In some of these embodiments, the fully automated virus extraction system 10 further includes a lifting mechanism. The lifting mechanism is connected to the first pipeline 910 and the second pipeline 920. The lifting mechanism is used for driving the first pipeline 910 and the second pipeline 920 to move up and down so as to respectively realize the butt joint and separation of the corresponding first centrifuge bottle 210 and the corresponding second centrifuge bottle 220.
In some of these embodiments, the lifting mechanism may be a drive cylinder, a drive motor, a screw assembly, or the like.
In some of these embodiments, the fully automated virus extraction system 10 further comprises a housing structure. The first liquid preparation tank 110, the second liquid preparation tank 120, the standard liquid container 130, the water storage container, the first centrifuge bottle 210, the second centrifuge bottle 220, the ultrasonic centrifugal oscillation multifunctional integrated machine 300, the first suction filter 410, the second suction filter 420, the vacuum equipment 500, the first suction filter 410, the second suction filter 420, the tangential flow device 700, the first receiving bucket 810, the second receiving bucket 820 and the waste container 830 are all arranged in a shell structure.
In some of these embodiments, referring to FIG. 1, the fully automated viral extraction system 10 further includes a stand 1100, the stand 1100 being disposed within the housing structure. The support 1100 comprises a multi-layer laminate. The first liquid preparation tank 110, the second liquid preparation tank 120, the standard liquid container 130, the water storage container, the first centrifuge bottle 210, the second centrifuge bottle 220, the ultrasonic centrifugal oscillation multifunctional integrated machine 300, the first suction filter 410, the second suction filter 420, the vacuum equipment 500, the first suction filter 410, the second suction filter 420, the tangential flow device 700, the first material receiving tank 810, the second material receiving tank 820 and the waste container 830 are respectively arranged on different laminates of the bracket 1100 according to the installation height.
In some of these embodiments, the fully automated virus extraction system 10 further comprises a controller. The first liquid adding pump 1010, the second liquid adding pump 1020, the third liquid adding pump 1030, the fourth liquid adding pump 1040, the fifth liquid adding pump 1050, the sixth liquid adding pump, the ultrasonic vibrator, the oscillator, the centrifuge 320, the first suction filter 410, the second suction filter 420, the vacuum apparatus 500, the tangential flow device 700, the refrigerator and the lifting mechanism are respectively connected with the controller by electrical signals. The controller may be a PLC programmable logic controller. The controller can set a corresponding control program according to the virus extraction flow.
In some of these embodiments, the fully automated virus extraction system 10 further includes an input display mechanism. The input display mechanism is arranged on the shell structure and is electrically connected with the controller through signals. The input display mechanism is used for performing man-machine interaction. The input display mechanism is used for displaying corresponding setting parameter data, corresponding process progress and the like. The input display mechanism is not shown in the drawings.
In some of these embodiments, the input display mechanism may be a touch screen.
In some of these embodiments, the fully automated virus extraction system 10 further includes a power supply device. The power supply device is connected to the first liquid adding pump 1010, the second liquid adding pump 1020, the third liquid adding pump 1030, the fourth liquid adding pump 1040, the fifth liquid adding pump 1050, the sixth liquid adding pump, the ultrasonic vibrator, the oscillator, the centrifuge 320, the first suction filter 410, the second suction filter 420, the vacuum device 500, the tangential flow device 700, the refrigerator, and the lifting mechanism. The power supply device is not shown in the drawings.
An embodiment of the application also provides a full-automatic virus extraction method.
A fully automatic virus extraction method, employing a fully automatic virus extraction system 10, comprising the steps of:
(1) Weighing; the viral sample sediment is weighed into a centrifuge vessel.
(2) Adding the leaching solution into the centrifugal container in the step (1), and shaking uniformly; the leaching solution is prepared by dissolving 1-2wt% of bovine serum albumin in 0.01M PBS buffer solution; the mass ratio of the added viral sample sediment to the leaching solution is 1:3-1:10.
(3) Standing in darkness; the standard solution container 130 is controlled to add standard solution to the centrifugal container, and the centrifugal container is kept stand in a dark environment for at least 15 min after being trimmed in the ultrasonic centrifugal oscillating multifunctional integrated machine 300.
(4) Ultrasonic treatment; and controlling the water storage container to add water into the ultrasonic centrifugal vibration multifunctional integrated machine 300, and controlling the ultrasonic centrifugal vibration multifunctional integrated machine 300 to carry out ultrasonic treatment for at least 15 min under the conditions of 40-50W and 4-8 ℃.
(5) Oscillating; and controlling the ultrasonic centrifugal oscillating multifunctional integrated machine 300 to oscillate at least 1 h at the rotating speed of 250 rpm-300 rpm.
(6) Centrifuging; the ultrasonic centrifugal oscillating multifunctional integrated machine 300 is controlled to centrifuge at 8000 rpm for at least 10 min.
(7) Suction filtration; and collecting supernatant liquid in the centrifuged container, pumping the supernatant liquid to a suction filtration device, controlling the vacuum equipment 500 to work, enabling the supernatant liquid to sequentially pass through a filter membrane of the suction filtration device, removing large particles and microorganisms, and collecting filtrate and then entering the suction filtration container.
(8) Tangential flow filtration.
1) Cleaning the tangential flow device 700;
2) Sample adding; and opening the suction filtration container, opening an upper permeation pipeline valve and a reflux valve of the tangential flow device 700 connected with the suction filtration container, allowing filtrate in the suction filtration container to enter the tangential flow device 700, filtering concentrated virus suspension at a flow rate of 20% -40%, allowing the concentrated solution to enter a corresponding receiving container, and allowing waste liquid in the tangential flow device 700 to enter a waste liquid container.
The full-automatic virus extraction method is carried out at the temperature of 4-8 ℃ in the whole process.
In some of these embodiments, the following method describes a fully automated virus extraction method in terms of two centrifuge bottles, such as first centrifuge bottle 210, second centrifuge bottle 220, wherein first centrifuge bottle 210, second centrifuge bottle 220 are in a trim symmetry state within ultrasound tank 310.
A full-automatic virus extraction method comprises the following steps:
(1) And (5) weighing the sample. Two aliquots of the 70 g virus sample sediment were manually weighed into the first 210 and second 220 centrifuge bottles of 250 mL, respectively.
(2) Adding leaching solution into the first centrifugal bottle 210 and the second centrifugal bottle 220 in the step (1), and shaking uniformly: the first drive pump is controlled to pump a predetermined volume of leach liquor from the first liquor dispensing tank 110 to the first centrifuge bottle 210 and the second drive pump is controlled to pump an equal volume of leach liquor from the second liquor dispensing tank 120 to the second centrifuge bottle 220. The leaching solution is prepared by dissolving 1-2 wt% of bovine serum albumin in 0.01M PBS buffer solution. It should be noted that the mass ratio of the added viral sample sediment to the leaching solution was 1:3, and the concentration of the leaching solution was 1.067 times the final concentration because the viral sample sediment contained 20% flowing water, and the added amount was subtracted from the viral sample sediment flowing water.
(3) And (5) standing in dark. The third liquid feeding pump 1030 is controlled to feed the standard liquid having a volume of 0 to 200mL from the standard liquid container 130 to the first centrifuge bottle 210 and the second centrifuge bottle 220, respectively. The first centrifuge bottle 210 and the second centrifuge bottle 220 are allowed to stand in a dark environment for at least 15 minutes.
(4) And (5) ultrasonic treatment. And controlling a sixth liquid adding pump to add water into the ultrasonic barrel body 310 from the water storage container, and controlling the ultrasonic vibrator to work, wherein the ultrasonic vibrator is subjected to ultrasonic treatment for at least 15 min at the temperature of 40W and the temperature of 4-8 ℃.
(5) Oscillating. The oscillator is controlled to oscillate the centrifugal base and the ultrasonic barrel 310 connected with the centrifugal base by at least 1 h at the rotation speed of 250 rpm-300 rpm.
(6) And (5) centrifuging. And (3) controlling the centrifugal machine 320 to centrifuge the ultrasonic barrel body 310 after the vibration in the step (5) at the rotating speed of 8000 rpm for at least 10 min.
(7) And (5) suction filtration. The fourth liquid adding pump 1040 is controlled to pump the supernatant collected and centrifuged in the first centrifuge bottle 210 to the first suction filter 410, and the first vacuum pump is controlled to work, so that the supernatant sequentially passes through the 2 μm and 0.22 μm filter membranes of the first suction filter 410, large particles and microorganisms are removed, and the filtrate is collected and then enters the first suction filter 410.
The fifth liquid feeding pump 1050 is controlled to pump the supernatant collected and centrifuged in the second centrifuge bottle 220 to the second suction filter 420, and the second vacuum pump is controlled to work so that the supernatant sequentially passes through the 2 μm and 0.22 μm filter membranes of the second suction filter 420 to remove large particles and microorganisms, and the filtrate is collected and then enters the second suction filter 420.
(8) Tangential flow filtration.
1) And cleaning the membrane bag. Specifically, a 0.2M NaOH solution is prepared, the feed tank of the tangential flow device 700 is filled, the solution is made to flow through the membrane bag at a flow rate of 20% -40%, all the pipelines of the tangential flow device 700 are cleaned, after 2 min, other pipelines are closed, only the valves of the upper permeation pipelines are kept open, after all NaOH flows out, sterilizing water is added, all the pipelines of the tangential flow device 700 are cleaned, and finally all the pipelines of the tangential flow device 700 are cleaned by 2% bovine serum albumin.
2) And (5) sample adding. The first suction filter 410 is opened, an upper permeation pipeline valve and a reflux valve of the tangential flow device 700 connected with the first suction filter 410 are opened, filtrate in the first suction filter 410 is added into the corresponding tangential flow device 700, concentrated virus suspension is filtered at a flow rate of 20% -40%, the concentrated solution enters the corresponding first material receiving barrel 810, waste liquid in the corresponding tangential flow device 700 enters the waste liquid barrel, and the operation is stopped after the sample in the first material receiving barrel 810 is concentrated to a required volume, and the whole process is controlled to operate at 4 ℃ -8 ℃.
And opening the second suction filter 420, opening an upper permeation pipeline valve and a reflux valve of the tangential flow device 700 connected with the second suction filter 420, adding filtrate in the second suction filter 420 into the corresponding tangential flow device 700, filtering concentrated virus suspension at a flow rate of 20% -40%, feeding the concentrated solution into the corresponding second material receiving barrel 820, feeding waste liquid in the corresponding tangential flow device 700 into the waste liquid barrel, stopping running after the sample in the second material receiving barrel 820 is concentrated to a required volume, and controlling the whole flow to operate at 4 ℃ -8 ℃.
In summary, the fully automatic virus extraction system 10 of the present invention can implement three-in-one innovation of liquid oscillation, centrifugation and ultrasound in one or more centrifugation containers, such as the first centrifugation bottle 210 and the second centrifugation bottle 220, so as to improve the virus extraction experimental efficiency, and the full-process automatic operation of virus extraction, and highly automate the experimental steps of manual and trivial operation of operators in the conventional technology, so that the process operation is more convenient, the manual extraction error is reduced, and the virus extraction effect and virus activity are ensured to the greatest extent. Specifically, the full-automatic virus extraction system 10 realizes full-automatic procedures from dosing, extraction, vibration, centrifugation, filtration, enrichment and concentration to back flushing, greatly reduces manual participation of operators, on one hand, liberates manual operation of the operators, on the other hand, improves the accuracy and uniformity of virus liquid extraction, can realize low-temperature storage of samples, avoids influence of excessive environmental temperature on virus extraction effect, and can more accurately reflect the composition characteristics of viruses in actual environments.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to related descriptions of other embodiments.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (10)
1. The full-automatic virus extraction system is characterized by comprising a liquid preparation container, a standard liquid container, a water storage container, a centrifugal container, an ultrasonic centrifugal oscillating multifunctional integrated machine, a suction filtration device, vacuum equipment, a suction filtration container, a tangential flow device, a material receiving container and a waste container; the liquid preparation container is detachably connected to the centrifugal container through a pipeline, the standard liquid container is connected to the centrifugal container for adding standard liquid into the centrifugal container, the centrifugal container is arranged in the ultrasonic centrifugal oscillating multifunctional integrated machine and is in balancing arrangement with a balancing piece, the ultrasonic centrifugal oscillating multifunctional integrated machine can realize ultrasonic, centrifugal and oscillating functions in situ, the centrifugal container is connected to the suction filtration device, the suction filtration device is connected to the suction filtration container, the suction filtration container is connected to the tangential flow device, the suction filtration container is connected to the vacuum equipment, the tangential flow device is connected to the material receiving container and the waste container, and the water storage container is connected to the ultrasonic centrifugal oscillating multifunctional integrated machine for adding water into the ultrasonic centrifugal oscillating multifunctional integrated machine for ultrasound preparation;
the ultrasonic centrifugal oscillating multifunctional integrated machine comprises an ultrasonic barrel body, an ultrasonic vibrator, an oscillator and a centrifugal machine, wherein a plurality of centrifugal containers or centrifugal containers and balancing pieces are symmetrically arranged in the ultrasonic barrel body through balancing, the ultrasonic vibrator is arranged in the ultrasonic barrel body to carry out ultrasonic treatment on liquid in the centrifugal containers in the ultrasonic barrel body, the centrifugal machine is arranged on a centrifugal base and used for centrifuging the ultrasonic barrel body, and the oscillator is connected with the centrifugal base and used for oscillating the centrifugal base and the ultrasonic barrel body connected with the centrifugal base;
the full-automatic virus extraction system further comprises a lifting mechanism, wherein the lifting mechanism is connected to the pipeline and is used for driving the pipeline to move up and down so as to realize the butt joint and separation with the centrifugal container; the liquid preparation container comprises a first liquid preparation barrel and a second liquid preparation barrel; the centrifugal container comprises a first centrifugal bottle and a second centrifugal bottle; the suction filtration device comprises a first suction filtration machine and a second suction filtration machine; the suction filtration container comprises a first suction filtration barrel and a second suction filtration barrel; the material receiving container comprises a first material receiving barrel and a second material receiving barrel;
the first liquid preparation barrel is detachably connected to the first centrifugal bottle through a first pipeline, the second liquid preparation barrel is detachably connected to the second centrifugal bottle through a second pipeline, the standard liquid container is simultaneously connected to the first centrifugal bottle and the second centrifugal bottle for respectively adding standard liquid into the first centrifugal bottle and the second centrifugal bottle, the first centrifugal bottle and the second centrifugal bottle are both arranged in the ultrasonic centrifugal oscillating multifunctional integrated machine, the first centrifugal bottle is connected to the first suction filter, the second centrifugal bottle is connected to the second suction filter, the first suction filter is connected to the first suction filter barrel, the second suction filter is connected to the second suction filter barrel, the first suction filter barrel and the second suction filter barrel are respectively connected to the tangential flow devices, the first suction filter and the second suction filter are respectively connected to the vacuum equipment, and the two tangential flow devices are respectively connected to the first suction filter and the second suction filter;
the full-automatic virus extraction system further comprises a controller and an input display mechanism, wherein the ultrasonic vibrator, the oscillator, the centrifugal machine, the first suction filter, the second suction filter, the vacuum equipment, the tangential flow device and the lifting mechanism are respectively connected with the controller through electrical signals, and the input display mechanism is connected with the controller through electrical signals and is used for performing man-machine interaction.
2. The fully automatic virus extraction system of claim 1, further comprising a first liquid feed pump, a second liquid feed pump;
the first liquid preparation barrel is connected to the first centrifugal bottle through the first liquid adding pump, and the second liquid preparation barrel is connected to the second centrifugal bottle through the second liquid adding pump.
3. The fully automatic virus extraction system of claim 1, wherein the vacuum apparatus comprises a first vacuum pump, a second vacuum pump, the first suction filter being connected to the first vacuum pump, the second suction filter being connected to the second vacuum pump.
4. The fully automatic virus extraction system of claim 1, further comprising a third liquid feed pump; the standard solution container is connected to the centrifugal container through the third liquid adding pump for adding standard solution to the centrifugal container.
5. The fully automatic virus extraction system of claim 1, further comprising a fourth liquid feed pump; the centrifugal container is connected with the suction filtration device through the fourth liquid adding pump.
6. The fully automatic virus extraction system according to any one of claims 1 to 5, further comprising a sixth liquid feeding pump; the water storage container is connected to the ultrasonic centrifugal oscillation multifunctional integrated machine through the sixth liquid adding pump and is used for adding water into the ultrasonic centrifugal oscillation multifunctional integrated machine for preparing ultrasonic waves.
7. The fully automatic virus extraction system according to any one of claims 1 to 5, further comprising a refrigerator for providing a temperature control function for the fully automatic virus extraction system.
8. The fully automatic virus extraction system according to any one of claims 1 to 5, wherein the lifting mechanism is a drive cylinder, a drive motor or a screw assembly.
9. The fully automatic virus extraction system according to any one of claims 1 to 5, wherein the controller is a PLC programmable logic controller and the input display mechanism is a touch screen.
10. A fully automatic virus extraction method, characterized in that the fully automatic virus extraction system according to any one of claims 1 to 9 is adopted, comprising the following steps:
(1) Weighing; weighing a virus sample sediment in a centrifugal container;
(2) Adding the leaching solution into the centrifugal container in the step (1), and shaking uniformly; the leaching solution is prepared by dissolving 1-2wt% of bovine serum albumin in 0.01M PBS buffer solution; the mass ratio of the added virus sample sediment to the leaching solution is 1:3-1:10;
(3) Standing in darkness; controlling a standard liquid container to add an equal volume of standard liquid into a centrifugal container, balancing the centrifugal container in an ultrasonic centrifugal oscillating multifunctional integrated machine, and standing in a dark environment for at least 15 min;
(4) Ultrasonic treatment; controlling the water storage container to add water into the ultrasonic centrifugal vibration multifunctional integrated machine, and controlling the ultrasonic centrifugal vibration multifunctional integrated machine to carry out ultrasonic treatment for at least 15 min at the temperature of 40-50W and the temperature of 4-8 ℃;
(5) Oscillating; controlling the ultrasonic centrifugal oscillating multifunctional integrated machine to oscillate at least 1 h at the rotating speed of 250 rpm-300 rpm;
(6) Centrifuging; controlling the ultrasonic centrifugal oscillating multifunctional integrated machine to centrifuge for at least 10 min at 8000 rpm;
(7) Suction filtration; collecting supernatant liquid in the centrifuged container, pumping the supernatant liquid to a suction filtration device, controlling vacuum equipment to work, enabling the supernatant liquid to sequentially pass through a filter membrane of the suction filtration device, removing large particles and microorganisms, collecting filtrate, and then entering the suction filtration container;
(8) Tangential flow filtration;
1) Cleaning the tangential flow device;
2) Sample adding; and opening the suction filtration container, opening an upper permeation pipeline valve and a reflux valve of the tangential flow device connected with the suction filtration container, allowing filtrate in the suction filtration container to enter the tangential flow device, filtering concentrated virus suspension at a flow rate of 20% -40%, allowing the concentrated solution to enter a corresponding receiving container, and allowing waste liquid in the tangential flow device to enter a waste liquid container.
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