CN117405824A - Nicotine content determination kit and rapid detection method - Google Patents
Nicotine content determination kit and rapid detection method Download PDFInfo
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- CN117405824A CN117405824A CN202311716876.1A CN202311716876A CN117405824A CN 117405824 A CN117405824 A CN 117405824A CN 202311716876 A CN202311716876 A CN 202311716876A CN 117405824 A CN117405824 A CN 117405824A
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- nicotine
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- acid
- buffer solution
- glacial acetic
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- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 83
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 title claims abstract description 78
- 229960002715 nicotine Drugs 0.000 title claims abstract description 76
- 238000001514 detection method Methods 0.000 title abstract description 25
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 94
- 239000000243 solution Substances 0.000 claims abstract description 73
- 239000007853 buffer solution Substances 0.000 claims abstract description 58
- 229960000583 acetic acid Drugs 0.000 claims abstract description 46
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 44
- 239000013078 crystal Substances 0.000 claims abstract description 25
- 238000011161 development Methods 0.000 claims abstract description 24
- 239000002904 solvent Substances 0.000 claims abstract description 24
- 238000002360 preparation method Methods 0.000 claims description 18
- 238000005303 weighing Methods 0.000 claims description 12
- 150000008064 anhydrides Chemical class 0.000 claims description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 150000007524 organic acids Chemical class 0.000 claims description 6
- JOOXCMJARBKPKM-UHFFFAOYSA-N 4-oxopentanoic acid Chemical compound CC(=O)CCC(O)=O JOOXCMJARBKPKM-UHFFFAOYSA-N 0.000 claims description 4
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 claims description 4
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 claims description 4
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 239000005711 Benzoic acid Substances 0.000 claims description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- 235000016623 Fragaria vesca Nutrition 0.000 claims description 2
- 240000009088 Fragaria x ananassa Species 0.000 claims description 2
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims description 2
- 239000004471 Glycine Substances 0.000 claims description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 235000010233 benzoic acid Nutrition 0.000 claims description 2
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 claims description 2
- 238000004364 calculation method Methods 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 239000001530 fumaric acid Substances 0.000 claims description 2
- 235000011087 fumaric acid Nutrition 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 229940040102 levulinic acid Drugs 0.000 claims description 2
- 229940107700 pyruvic acid Drugs 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 2
- 238000003149 assay kit Methods 0.000 claims 5
- 238000003556 assay Methods 0.000 claims 3
- 238000000034 method Methods 0.000 abstract description 24
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 3
- 239000003571 electronic cigarette Substances 0.000 description 18
- 239000000523 sample Substances 0.000 description 17
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 8
- 241000208125 Nicotiana Species 0.000 description 7
- JKYKXTRKURYNGW-UHFFFAOYSA-N 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C(O)=C(O)C(S(O)(=O)=O)=C2 JKYKXTRKURYNGW-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000004448 titration Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 3
- MLVYOYVMOZFHIU-UHFFFAOYSA-M sodium;4-[(4-anilinophenyl)diazenyl]benzenesulfonate Chemical compound [Na+].C1=CC(S(=O)(=O)[O-])=CC=C1N=NC(C=C1)=CC=C1NC1=CC=CC=C1 MLVYOYVMOZFHIU-UHFFFAOYSA-M 0.000 description 3
- VWTHFJXLFGINSW-PPHPATTJSA-N 2-hydroxypropanoic acid;3-[(2s)-1-methylpyrrolidin-2-yl]pyridine Chemical compound CC(O)C(O)=O.CN1CCC[C@H]1C1=CC=CN=C1 VWTHFJXLFGINSW-PPHPATTJSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000004365 square wave voltammetry Methods 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 238000002479 acid--base titration Methods 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- -1 salt compounds Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention relates to a nicotine content determination kit and a rapid detection method, and belongs to the technical field of nicotine detection. The kit comprises buffer solution, color development solution and solvent; the buffer solution is a perchloric acid solution, the developing solution is a crystal violet solution, and the solvent is anhydrous glacial acetic acid. The invention is based on the principle that nicotine reacts with perchloric acid in buffer solution, color change occurs in color developing solution at the end point of reaction, and the amount of buffer solution added is recorded, so that the nicotine content is calculated. The method used by the invention has the advantages of short time consumption, no need of large equipment, short measurement time, time saving and labor saving, good repeatability and relative standard deviation within 2.0 percent.
Description
Technical Field
The invention relates to a nicotine content determination kit and a rapid detection method, and belongs to the technical field of nicotine detection.
Background
Nicotine is an alkaline compound naturally occurring in tobacco plants and is also one of the major alkaloids in tobacco. It can cause a series of physiological and neural reactions after being inhaled into human body by means of smog, and can produce addictive and sedative effects. Nicotine salts are salt compounds formed by combining nicotine with organic acids and are widely used in tobacco products. Nicotine salts have a lower pH, provide a smoother inhalation experience, and reduce throat and oral irritation. The content of nicotine in the electronic cigarette oil is about 2-5%, and the electronic cigarette oil is heated and atomized by the heating device to enter a human body, so that the nicotine in the electronic cigarette oil needs to be accurately detected in order to ensure the quality and safety of products such as the tobacco oil.
The quantitative analysis of nicotine is a process for determining the content of nicotine, and common quantitative analysis methods include gas chromatography-mass spectrometry, high performance liquid chromatography and the like.
The gas chromatography-mass spectrometry combines the gas chromatography and mass spectrometry technologies, extracts nicotine in a sample by methods of evaporation, dissolution and the like, and adds a proper solvent for dilution. The nicotine content can then be determined by measurement and analysis by gas chromatograph mass spectrometry and compared to known standard samples to obtain accurate results.
The high performance liquid chromatography separates nicotine from the sample by a solvent and quantitatively analyzes the separated target substance by a detector. Dissolving and diluting a sample, injecting the sample into a high performance liquid chromatograph through a sample injector, pumping into a chromatographic column filled with a stationary phase, separating each component in the column, detecting and quantitatively analyzing the separated target substance through a detector, and determining the content of nicotine by establishing a standard curve.
CN114624303B discloses an electrochemical method for rapid quantitative determination of nicotine in electronic cigarettes, which comprises the following steps: (1) pretreatment of a working electrode; (2) preparing a nicotine standard solution and drawing a standard curve; (3) determination of an e-cigarette sample: measuring the tobacco tar of the electronic cigarettes with different volumes, and adding organic acid and inorganic acid to obtain electronic cigarette samples with different dilution factors; recording an SWV curve of the electronic cigarette sample by a square wave voltammetry; and calculating to obtain the nicotine content value in the electronic cigarette sample solution, and multiplying the nicotine content value by the corresponding dilution times to obtain the nicotine content in the sample. The method requires a chemical workstation, is difficult to walk out of a laboratory for detection, still depends on equipment for detection, is complex in time-consuming rectangular method, and requires long training to operate.
CN114486869a discloses a qualitative or quantitative detection method for nicotine components in electronic cigarette tobacco tar based on color change, which uses Alizarin Red S (ARS) to react with sodium hydroxide to generate alizarin red S, the alizarin red S can react with nicotine components in the electronic cigarette tobacco tar in the form of nicotine salt in a color development way, the color change can be identified by naked eyes to judge whether the tobacco tar contains nicotine components, and quantitative detection for nicotine components can be realized by detecting the change of absorbance value of a solution. The method is used for qualitatively detecting nicotine through color change, but quantitative detection cannot be realized through color change, and the quantitative detection still needs equipment to measure the absorbance value of the solution, so that the cost is high and the method is complex.
In the detection method in the prior art, a large instrument is usually needed, the detection method is difficult to leave a laboratory, the response time of the gas chromatography-mass spectrometry combination method and other methods without pretreatment and other operations exceeds 10 minutes, the operation is complex, the numerical value can be determined after a large number of data records or comparison, an operator can perform detection work after long-term training, the time consumption is long, the operation difficulty is high, even a portable electrochemical workstation is very often different from tens of thousands to hundreds of thousands, and the cost is high and the detection method is difficult to popularize.
Disclosure of Invention
In order to solve the problems that in the prior art, the qualitative and quantitative detection of nicotine is needed by means of an instrument, the detection time is long, the operation is complex, and the labor cost is high, the invention provides a nicotine content detection kit and a rapid detection method.
The invention provides a nicotine content determination kit, which comprises buffer solution, color development solution and solvent; the buffer solution is a perchloric acid solution, the developing solution is a crystal violet solution, and the solvent is anhydrous glacial acetic acid.
Further, the buffer concentration is 0.02mol/L to 0.1mol/L.
And further, the preparation method of the buffer solution comprises the steps of taking 750mL of anhydrous glacial acetic acid, adding 8.5mL of perchloric acid with the concentration of 70% -72%, slowly dropwise adding 23mL of anhydride at room temperature after shaking, shaking while adding, shaking uniformly after adding, cooling to room temperature, adding the anhydrous glacial acetic acid to 1000mL, shaking uniformly, standing for 24 hours to obtain 0.1mol/L of buffer solution, and adding the anhydrous glacial acetic acid into the buffer solution with the concentration of 0.1mol/L to dilute to the required concentration.
Further, the concentration of the crystal violet solution was 5mg/mL, and the preparation method was such that 0.5g of crystal violet was dissolved in 100mL of anhydrous glacial acetic acid.
The rapid detection method of the nicotine content determination kit comprises the following steps:
s1, weighing an object to be measured, recording a weighing sample amount m, adding the object to be measured into 50mL of anhydrous glacial acetic acid solvent, dissolving and shaking uniformly;
s2, adding a color developing solution into the solution obtained in the step S1 while shaking, and shaking uniformly;
s3, slowly dropwise adding a buffer solution into the solution obtained in the step S2 while shaking until the color of the solution turns green, and recording the volume V of the added buffer solution;
s4, calculating the content of nicotine.
Further, the volume of the color-developing solution added in the step S2 is 0.2mL.
Further, the formula of calculating the nicotine content in step S4 is as follows:
wherein m is the sample weighing amount of the to-be-detected object, the unit is mg, V is the volume of the added buffer solution, the unit is mL, n is the molar concentration of the buffer solution, and the unit is mol/L.
Furthermore, the nicotine content determination kit or the rapid detection method is applied to the detection of the nicotine content in nicotine, nicotine salt samples or e-tar, wherein the nicotine salt is formed by organic acid and nicotine, and the organic acid comprises one or more of benzoic acid, lactic acid, levulinic acid, fumaric acid, tartaric acid, citric acid, strawberry acid, pyruvic acid, succinic acid, glycolic acid or glycine.
The invention provides a nicotine content determination kit, wherein reagents in the kit are filled in containers with different specifications, such as centrifuge tubes, sealing bottles and the like, so that the kit is convenient to take and has sealing effect, and the kit also comprises a sampler, such as a disposable dropper and the like, so that a sample can be conveniently titrated.
The invention is based on the principle that nicotine reacts with perchloric acid in buffer solution, color change occurs in color developing solution at the end point of reaction, and the amount of buffer solution added is recorded, so that the nicotine content is calculated. At present, no indicator method nicotine detection kit and no indicator method exist on the market.
Crystal violet was used as a visual indicator and when dropped in glacial acetic acid, a clear and rapid color change was produced. The method comprises the steps of adding a solution of perchloric acid with the concentration of 0.02mol/L-0.1mol/L as a solvent, and then adding the solution of perchloric acid into a solution, wherein the solution of perchloric acid is dissolved in the solution of acetic acid to form a solution of perchloric acid, and the solution of perchloric acid is dissolved in the solution of acetic acid to form an aqueous solution of perchloric acid.
The nicotine content determination kit and the rapid detection method provided by the invention only need buffer solution, color development liquid and solvent, and the preparation methods of the three reagents are relatively simple; the color change of the crystal violet at the titration end point has higher sensitivity, the titration end point can be directly observed by naked eyes, the determination of the titration result is more visual and convenient, the acid-base titration method is short in time consumption, a long-time measurement process is not needed, time and labor are saved, the repeatability is good, and the relative standard deviation is within 2.0%. In the prior art, a portable, simple and quick nicotine content determination kit does not exist, the kit enables nicotine to be detected to leave a laboratory, the time consumed by a titration mode is far less than that of a method requiring large-scale instrument equipment such as an electrochemical detection method or a gas chromatography, the subsequent calculation mode is simple, the using method is simple, the using threshold is reduced, and the nicotine content determination kit can be applied to various laboratories or on-site determination work of nicotine content in nicotine and electronic cigarette tobacco tar and is very suitable for large-area popularization.
Detailed Description
The technical scheme and effects of the invention are further described by the following specific examples. The following examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. Simple modifications of the invention using the inventive concept are within the scope of the invention as claimed.
The apparatus used in the preparation method of the present invention may employ any apparatus known in the art. The raw materials used in the present invention are commercially available unless otherwise indicated.
Example 1
A nicotine content determination kit comprises buffer solution, color development solution and solvent.
The buffer solution is perchloric acid solution with the concentration of 0.1mol/L, and the preparation method comprises the following steps: and (3) taking 750mL of anhydrous glacial acetic acid, adding 8.5mL of perchloric acid with the concentration of 70% -72%, shaking uniformly, slowly dropwise adding 23mL of anhydride at room temperature, shaking simultaneously, shaking uniformly after adding, standing to room temperature, adding the anhydrous glacial acetic acid to 1000mL, shaking uniformly, and standing for 24h.
The preparation method of the crystal violet solution with the color development solution concentration of 5mg/mL comprises the following steps: 0.5g of crystal violet was taken and 100mL of anhydrous glacial acetic acid was added thereto to dissolve the crystal violet.
The solvent is anhydrous glacial acetic acid.
Buffer solution is added into a No. 1 centrifuge tube with the specification of 100mL, color development solution is added into a No. 2 centrifuge tube with the specification of 5mL, and 50mL of anhydrous glacial acetic acid is added into a No. 3 centrifuge tube with the specification of 100 mL.
The method comprises the following specific steps:
s1, taking a No. 3 centrifuge tube, weighing 60mg of nicotine, adding the nicotine into the No. 3 centrifuge tube, dissolving and shaking uniformly.
S2, adding 0.2mL of color developing solution into the centrifuge tube No. 3 by using a disposable dropper, and shaking uniformly.
S3, dropwise adding the buffer solution into the No. 3 centrifuge tube while shaking until the color of the sample in the No. 3 centrifuge tube is green, stopping adding the buffer solution, and recording that the volume of the added buffer solution is 7.4mL.
S4, calculating the content of nicotine as follows:。
example 2
A nicotine content determination kit comprises buffer solution, color development solution and solvent.
The buffer solution is perchloric acid solution with the concentration of 0.1mol/L, and the preparation method comprises the following steps: and (3) taking 750mL of anhydrous glacial acetic acid, adding 8.5mL of perchloric acid with the concentration of 70% -72%, shaking uniformly, slowly dropwise adding 23mL of anhydride at room temperature, shaking simultaneously, shaking uniformly after adding, standing to room temperature, adding the anhydrous glacial acetic acid to 1000mL, shaking uniformly, and standing for 24h.
The preparation method of the crystal violet solution with the color development solution concentration of 5mg/mL comprises the following steps: 0.5g of crystal violet was taken and 100mL of anhydrous glacial acetic acid was added thereto to dissolve the crystal violet.
The solvent is anhydrous glacial acetic acid.
Buffer solution is added into a No. 1 centrifuge tube with the specification of 100mL, color development solution is added into a No. 2 centrifuge tube with the specification of 5mL, and 50mL of anhydrous glacial acetic acid is added into a No. 3 centrifuge tube with the specification of 100 mL.
The method comprises the following specific steps:
s1, taking a No. 3 centrifuge tube, weighing 160mg of nicotine tartrate, adding the nicotine tartrate into the No. 3 centrifuge tube, dissolving and shaking uniformly.
S2, adding 0.2mL of color developing solution into the centrifuge tube No. 3 by using a disposable dropper, and shaking uniformly.
S3, dropwise adding the buffer solution into the No. 3 centrifuge tube while shaking until the color of the sample in the No. 3 centrifuge tube is green, stopping adding the buffer solution, and recording that the volume of the added buffer solution is 6.9mL.
S4, calculating the content of nicotine as follows:。
example 3
A nicotine content determination kit comprises buffer solution, color development solution and solvent.
The buffer solution is perchloric acid solution with the concentration of 0.07mol/L, and the preparation method comprises the following steps: and (3) taking 750mL of anhydrous glacial acetic acid, adding 8.5mL of perchloric acid with the concentration of 70% -72%, shaking uniformly, slowly dropwise adding 23mL of anhydride at room temperature, shaking simultaneously, shaking uniformly after adding, standing to room temperature, adding the anhydrous glacial acetic acid to 1000mL, shaking uniformly, standing for 24h, taking 700mL of the solution, and adding the anhydrous glacial acetic acid to 1000mL.
The preparation method of the crystal violet solution with the color development solution concentration of 5mg/mL comprises the following steps: 0.5g of crystal violet was taken and 100mL of anhydrous glacial acetic acid was added thereto to dissolve the crystal violet.
The solvent is anhydrous glacial acetic acid.
Buffer solution is added into a No. 1 centrifuge tube with the specification of 100mL, color development solution is added into a No. 2 centrifuge tube with the specification of 5mL, and 50mL of anhydrous glacial acetic acid is added into a No. 3 centrifuge tube with the specification of 100 mL.
The method comprises the following specific steps:
s1, taking a No. 3 centrifuge tube, weighing 110mg of nicotine lactate, adding the nicotine lactate into the No. 3 centrifuge tube, dissolving and shaking uniformly.
S2, adding 0.2mL of color developing solution into the centrifuge tube No. 3 by using a disposable dropper, and shaking uniformly.
S3, dropwise adding the buffer solution into the No. 3 centrifuge tube while shaking until the color of the sample in the No. 3 centrifuge tube is green, stopping adding the buffer solution, and recording that the volume of the added buffer solution is 6.2mL.
S4, calculating the content of nicotine as follows:。
example 4
A nicotine content determination kit comprises buffer solution, color development solution and solvent.
The buffer solution is perchloric acid solution with the concentration of 0.02mol/L, and the preparation method comprises the following steps: and (3) taking 750mL of anhydrous glacial acetic acid, adding 8.5mL of perchloric acid with the concentration of 70% -72%, shaking uniformly, slowly dropwise adding 23mL of anhydride at room temperature, shaking simultaneously, shaking uniformly after adding, standing to room temperature, adding the anhydrous glacial acetic acid to 1000mL, shaking uniformly, standing for 24h, taking 200mL of the solution, and adding the anhydrous glacial acetic acid to 1000mL.
The preparation method of the crystal violet solution with the color development solution concentration of 5mg/mL comprises the following steps: 0.5g of crystal violet was taken and 100mL of anhydrous glacial acetic acid was added thereto to dissolve the crystal violet.
The solvent is anhydrous glacial acetic acid.
Buffer solution is added into a No. 1 centrifuge tube with the specification of 100mL, color development solution is added into a No. 2 centrifuge tube with the specification of 5mL, and 50mL of anhydrous glacial acetic acid is added into a No. 3 centrifuge tube with the specification of 100 mL.
The method comprises the following specific steps:
s1, taking a No. 3 centrifuge tube, weighing 140mg of electronic cigarette oil, adding the electronic cigarette oil into the No. 3 centrifuge tube, dissolving and shaking uniformly.
S2, adding 0.2mL of color developing solution into the centrifuge tube No. 3 by using a disposable dropper, and shaking uniformly.
S3, dropwise adding the buffer solution into the No. 3 centrifuge tube while shaking until the color of the sample in the No. 3 centrifuge tube is green, stopping adding the buffer solution, and recording that the volume of the added buffer solution is 4.0mL.
S4, calculating the content of nicotine as follows:。
comparative example 1
A nicotine content determination kit comprises buffer solution, color development solution and solvent.
The buffer solution is hydrochloric acid solution with the concentration of 0.1mol/L, and the preparation method comprises the following steps: 9mL of hydrochloric acid was taken, water was added thereto to 1000mL, and the mixture was left to stand for 24 hours after shaking.
The preparation method of the crystal violet solution with the color development solution concentration of 5mg/mL comprises the following steps: 0.5g of crystal violet was taken and 100mL of anhydrous glacial acetic acid was added thereto to dissolve the crystal violet.
The solvent is anhydrous glacial acetic acid.
Buffer solution is added into a No. 1 centrifuge tube with the specification of 100mL, color development solution is added into a No. 2 centrifuge tube with the specification of 5mL, and 50mL of anhydrous glacial acetic acid is added into a No. 3 centrifuge tube with the specification of 100 mL.
The method comprises the following specific steps:
s1, taking a No. 3 centrifuge tube, weighing 140mg of electronic cigarette oil, adding the electronic cigarette oil into the No. 3 centrifuge tube, dissolving and shaking uniformly.
S2, adding 0.2mL of color developing solution into the centrifuge tube No. 3 by using a disposable dropper, and shaking uniformly.
S3, dropwise adding a buffer solution into the centrifuge tube No. 3 while shaking, wherein the color of the sample in the centrifuge tube No. 3 cannot indicate the end point, and the nicotine content cannot be calculated.
Comparative example 2
A nicotine content determination kit comprises buffer solution, color development solution and solvent.
The buffer solution is perchloric acid solution with the concentration of 0.1mol/L, and the preparation method comprises the following steps: and (3) taking 750mL of anhydrous glacial acetic acid, adding 8.5mL of perchloric acid with the concentration of 70% -72%, shaking uniformly, slowly dropwise adding 23mL of anhydride at room temperature, shaking simultaneously, shaking uniformly after adding, standing to room temperature, adding the anhydrous glacial acetic acid to 1000mL, shaking uniformly, and standing for 24h.
The preparation method of the orange IV solution with the color development solution concentration of 5mg/mL comprises the following steps: 0.5g of orange IV is taken, and 100mL of anhydrous glacial acetic acid is added to dissolve the orange IV.
The solvent is anhydrous glacial acetic acid.
Buffer solution is added into a No. 1 centrifuge tube with the specification of 100mL, color development solution is added into a No. 2 centrifuge tube with the specification of 5mL, and 50mL of anhydrous glacial acetic acid is added into a No. 3 centrifuge tube with the specification of 100 mL.
The method comprises the following specific steps:
s1, taking a No. 3 centrifuge tube, weighing 140mg of electronic cigarette oil, adding the electronic cigarette oil into the No. 3 centrifuge tube, dissolving and shaking uniformly.
S2, adding 0.2mL of color developing solution into the centrifuge tube No. 3 by using a disposable dropper, and shaking uniformly.
S3, dropwise adding a buffer solution into the centrifuge tube No. 3 while shaking, wherein the color of the sample in the centrifuge tube No. 3 cannot indicate the end point, and the nicotine content cannot be calculated.
Claims (8)
1. A nicotine content determination kit, characterized in that:
comprises buffer solution, color development solution and solvent; the buffer solution is a perchloric acid solution, the developing solution is a crystal violet solution, and the solvent is anhydrous glacial acetic acid.
2. A nicotine assay kit according to claim 1, characterized in that:
the concentration of the buffer solution is 0.02mol/L-0.1mol/L.
3. A nicotine content determination kit according to claim 2, characterized in that:
the preparation method of the buffer solution comprises the steps of taking 750mL of anhydrous glacial acetic acid, adding 8.5mL of perchloric acid with the concentration of 70% -72%, slowly dropwise adding 23mL of anhydride at room temperature after shaking, shaking while adding, shaking uniformly after adding, cooling to room temperature, adding the anhydrous glacial acetic acid to 1000mL, shaking uniformly, standing for 24h to obtain 0.1mol/L of buffer solution, and adding the anhydrous glacial acetic acid into the buffer solution with the concentration of 0.1mol/L to dilute to the required concentration.
4. A nicotine assay kit according to claim 1, characterized in that:
the concentration of the crystal violet solution is 5mg/mL, and the preparation method is that 0.5g of crystal violet is added with 100mL of anhydrous glacial acetic acid to dissolve the crystal violet.
5. A rapid assay method for nicotine content according to any one of claims 1-4, characterized by the specific steps of:
s1, weighing an object to be measured, recording a weighing sample amount m, adding the object to be measured into 50mL of anhydrous glacial acetic acid solvent, dissolving and shaking uniformly;
s2, adding a color developing solution into the solution obtained in the step S1 while shaking, and shaking uniformly;
s3, slowly dropwise adding a buffer solution into the solution obtained in the step S2 while shaking until the color of the solution turns green, and recording the volume V of the added buffer solution;
s4, calculating the content of nicotine.
6. The rapid assay method of nicotine content assay kit of claim 5, wherein:
the volume of the color development liquid added in the step S2 is 0.2mL.
7. The rapid assay method of nicotine content assay kit of claim 5, wherein:
the calculation formula of the nicotine content in the step S4 is as follows:wherein m is the sample weighing amount of the to-be-detected object, the unit is mg, V is the volume of the added buffer solution, the unit is mL, and n is the molar concentration of the buffer solution, and the unit is mol/L.
8. A nicotine assay kit according to claim 1, characterized in that:
the nicotine content determination kit is used for detecting the content of nicotine in nicotine, nicotine salt samples or e-tobacco tar, wherein the nicotine salt is formed by organic acid and nicotine, and the organic acid is one or more of benzoic acid, lactic acid, levulinic acid, fumaric acid, tartaric acid, citric acid, strawberry acid, pyruvic acid, succinic acid, glycolic acid or glycine.
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