CN117384730A - Nucleic acid cracking and amplifying device - Google Patents
Nucleic acid cracking and amplifying device Download PDFInfo
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- CN117384730A CN117384730A CN202210780642.2A CN202210780642A CN117384730A CN 117384730 A CN117384730 A CN 117384730A CN 202210780642 A CN202210780642 A CN 202210780642A CN 117384730 A CN117384730 A CN 117384730A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 54
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 54
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 54
- 238000005336 cracking Methods 0.000 title description 2
- 239000007788 liquid Substances 0.000 claims abstract description 224
- 239000002699 waste material Substances 0.000 claims abstract description 46
- 230000003321 amplification Effects 0.000 claims abstract description 28
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 28
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 27
- 230000007017 scission Effects 0.000 claims abstract description 27
- 239000003153 chemical reaction reagent Substances 0.000 claims description 50
- 238000006243 chemical reaction Methods 0.000 claims description 34
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 12
- 229910052782 aluminium Inorganic materials 0.000 claims description 12
- 239000011888 foil Substances 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 238000003825 pressing Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 5
- 238000007731 hot pressing Methods 0.000 claims description 3
- 239000000376 reactant Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 18
- 238000000034 method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 239000012445 acidic reagent Substances 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 3
- 238000011002 quantification Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a nucleic acid cleavage amplification device. The nucleic acid cleavage amplification device comprises: the device comprises a pushing device, a liquid storage device and a chip; the inside of the liquid storage device is provided with a plurality of chambers, the pushing device stretches into the inside of the liquid storage device, and the lower part of the pushing device is in sliding connection with the liquid storage device; one end of the liquid storage device is connected with the buckle above the pushing device, and the other end of the liquid storage device is connected with the chip buckle. The liquid storage device is communicated with the chip through the liquid inlet channel. The chip comprises: the chip body, ration groove, waste liquid groove and location support column, ration groove and waste liquid groove are equipped with a plurality ofly, offer ration groove and waste liquid groove on the chip body, and ration groove and waste liquid groove are respectively through ration runner and waste liquid runner intercommunication liquid storage device, and the location support column is established in one side of chip body bottom. The chip further includes: one end of the buffer flow channel is communicated with the quantitative flow channel, the other end of the buffer flow channel is communicated with the quantitative groove, and the sectional area of the buffer flow channel is smaller than that of the quantitative flow channel so as to improve the accuracy of nucleic acid detection.
Description
Technical Field
The invention relates to the field of nucleic acid cleavage amplification, in particular to a nucleic acid cleavage amplification device.
Background
In medicine, microfluidic chips are often used to detect nucleic acids. However, in use, due to the difference of detection indexes and items, multiple tests are often required for a single sample, so that the detection time is greatly increased. And the nucleic acid sample needs to be stored and transferred before detection, and after the sample is sent to a detection center, the nucleic acid sample and the reaction reagent are released into a detection instrument for nucleic acid detection. However, since there is no matching relationship between the liquid storage device and the detection apparatus, there is a case where the preservation of the nucleic acid sample is imperfect or the nucleic acid sample is missing during the transfer. Meanwhile, the nucleic acid storage device in the prior art has higher cost, and the process of releasing the liquid is complex, so that a plurality of collected samples cannot be released at the same time.
Meanwhile, as the chip is heated, when the freeze-dried reagent is pre-buried in the chip to react with the nucleic acid, the reagent is diluted after quantification.
Therefore, the nucleic acid detection equipment in the prior art has the technical problems that the cost is high, the operation is complex, the detection result is inaccurate due to the nucleic acid detection accuracy caused by heating amplification, and a plurality of embedded reagents cannot be released simultaneously.
Disclosure of Invention
Therefore, the invention mainly aims to provide a nucleic acid cleavage and amplification device which has low cost, simple operation, high detection accuracy and simultaneous release of a plurality of embedded reagents.
In order to achieve the above purpose, the technical scheme of the invention is realized as follows:
the nucleic acid cleavage amplification device comprises: the device comprises a pushing device, a liquid storage device and a chip; the inside of the liquid storage device is provided with a plurality of chambers, the pushing device stretches into the inside of the liquid storage device, and the lower part of the pushing device is in sliding connection with the liquid storage device; one end of the liquid storage device is connected with the buckle above the pushing device, and the other end of the liquid storage device is connected with the chip buckle;
the liquid storage device is communicated with the chip through a liquid inlet channel;
the chip comprises: the chip comprises a chip body, a quantitative tank, a waste liquid tank and positioning support columns, wherein a plurality of quantitative tanks and waste liquid tanks are arranged, the quantitative tank and the waste liquid tank are arranged on the chip body, the quantitative tank and the waste liquid tank are communicated with the liquid storage device through quantitative flow channels and waste liquid flow channels respectively, and the positioning support columns are arranged on one side of the bottom of the chip body;
the chip further includes: and one end of the buffer flow channel is communicated with the quantitative flow channel, the other end of the buffer flow channel is communicated with the quantitative groove, and the sectional area of the buffer flow channel is smaller than that of the quantitative flow channel.
In one embodiment, the pushing device comprises: the device comprises a pressing cover, a push rod and a piston, wherein the piston is fixedly connected to the tail part of the push rod, extends into the liquid storage device, and is in sliding connection with the liquid storage device, so that one end of the liquid storage device is sealed;
the pressing cover is arranged above the push rod and is in buckling connection with the liquid storage device.
In one embodiment, further comprising: and the limiting device is arranged between the push rod and the piston.
In one embodiment, the reservoir device comprises: the liquid storage device comprises a liquid storage device body, a sample liquid storage cavity and a reaction reagent liquid storage cavity, wherein the liquid storage device body is connected with a chip in a buckling manner, and the sample liquid storage cavity and the reaction reagent liquid storage cavity are formed in the liquid storage device body;
the upper end of the sample liquid storage cavity is sealed by a sealing cover, and the lower end of the sample liquid storage cavity is sealed by an aluminum foil; the upper end of the reaction reagent liquid storage cavity is sealed by a piston, and the lower end is sealed by an aluminum foil.
In one embodiment, the reservoir device further comprises: the reaction reagent capacity-expanding cavity is arranged in the liquid storage device body; one end of the reaction reagent expansion cavity is sealed by a piston, and the other end of the reaction reagent expansion cavity is sealed by a liquid storage device; the reaction reagent dilatation cavity is communicated with the sample liquid storage cavity.
In one embodiment, the chip is internally provided with a plurality of spike structures, and the spike structures are positioned below the sample liquid storage cavity and the reactant liquid storage cavity.
In one embodiment, the chip further comprises: the air holes are formed in the plurality of the air holes; the air holes are respectively communicated with the quantitative tank and the waste liquid tank through the flow channels.
In one embodiment, the liquid inlet channel is provided with a T-shaped channel opening, the quantitative tank and the waste liquid tank are respectively communicated with the T-shaped channel opening through the quantitative channel and the waste liquid channel, and the position relationship between the waste liquid channel and the liquid inlet channel is right angle;
the sectional area of the quantitative flow channel is far larger than that of the waste liquid flow channel.
In one embodiment, the lower ends of the sample liquid storage cavity and the reaction reagent liquid storage cavity are made of plastic materials, and the aluminum foil is bonded at the lower ends of the sample liquid storage cavity and the reaction reagent liquid storage cavity in a hot pressing mode.
In one embodiment, the sealing cover is made of plastic.
The nucleic acid cleavage and amplification device has the following beneficial effects:
the nucleic acid cleavage amplification device comprises: the device comprises a pushing device, a liquid storage device and a chip; the inside of the liquid storage device is provided with a plurality of chambers, the pushing device stretches into the inside of the liquid storage device, and the lower part of the pushing device is in sliding connection with the liquid storage device; one end of the liquid storage device is connected with the buckle above the pushing device, and the other end of the liquid storage device is connected with the chip buckle. The liquid storage device is communicated with the chip through the liquid inlet channel. The chip comprises: the chip body, ration groove, waste liquid groove and location support column, ration groove and waste liquid groove are equipped with a plurality ofly, offer ration groove and waste liquid groove on the chip body, and ration groove and waste liquid groove are respectively through ration runner and waste liquid runner intercommunication liquid storage device, and the location support column is established in one side of chip body bottom. The chip further includes: one end of the buffer flow channel is communicated with the quantitative flow channel, the other end of the buffer flow channel is communicated with the quantitative groove, and the sectional area of the buffer flow channel is smaller than that of the quantitative flow channel.
When the nucleic acid cleavage amplification device is used, the liquid storage device is connected with the chip through the buckle, then the buckle above the pushing device is extruded, and the pushing device is pressed downwards, so that liquid in a plurality of chambers inside the liquid storage device is released simultaneously. After releasing the nucleic acid reagent, the nucleic acid reagent enters the chip body through the liquid inlet channel for quantification, and the redundant reagent enters the waste liquid tank. In the liquid feeding process, the liquid reagent is mixed with the freeze-dried reagent pre-buried in the quantitative tank. After liquid is fed, the chip body is heated by the equipment, so that the liquid in the quantitative tank is amplified. The microfluidic chip for detecting nucleic acid is simple to operate and the detection time is reduced. In the heating process, the buffer flow channel above the quantitative groove has smaller sectional area and is positioned at the bottom, so that liquid in the flow channel can be quickly vaporized to form an air column, the quantitative groove and the liquid at the upstream are blocked, and the reagent concentration in the quantitative groove is prevented from being diluted, thereby achieving the technical effect of improving the accuracy of nucleic acid detection.
Meanwhile, the nucleic acid splitting and amplifying device is simple in structure and convenient to operate, and can simultaneously release a plurality of samples to be detected so as to achieve the technical effect that a plurality of samples are detected simultaneously.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is an exploded view of a nucleic acid cleavage amplification apparatus according to one embodiment of the present disclosure;
FIG. 2 is a front view of a nucleic acid cleavage amplification apparatus according to one embodiment of the present disclosure;
FIG. 3 is a right side view of a nucleic acid cleavage amplification apparatus according to one embodiment of the present disclosure;
FIG. 4 is a cross-sectional view taken along line A-A of FIG. 3;
FIG. 5 is a top view of a chip of one embodiment of the present disclosure;
FIG. 6 is a bottom view of a chip of one embodiment of the present disclosure;
FIG. 7 is a left side view of a chip of one embodiment of the present disclosure;
fig. 8 is a sectional view taken along line B-B of fig. 6.
[ Main reference numerals Specification ]
1. A pushing device; 11. pressing the cover; 12. a push rod; 13. a piston; 2. a liquid storage device; 21. a liquid storage device body; 22. a sample reservoir; 23. a reactant reservoir; 24. a reaction reagent expansion cavity; 3. a chip; 31. a chip body; 32. a quantitative tank; 33. a waste liquid tank; 34. positioning a support column; 35. a spike structure; 36. ventilation holes; 4. a liquid inlet flow channel; 5. a quantitative flow channel; 6. a waste liquid flow channel; 7. a buffer flow channel; 8. a limiting device; 9. sealing cover; 10. aluminum foil.
Detailed Description
The following describes a nucleic acid cleavage amplification apparatus according to the present invention in further detail with reference to the accompanying drawings and examples.
It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other. The invention will be described in detail below with reference to the drawings in connection with embodiments.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
It should be noted that the terms "first," "second," and the like in the description and claims of the present application and in the above-described figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the present application described herein may be capable of being practiced otherwise than as specifically illustrated and described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
Spatially relative terms, such as "above," "over," "upper surface," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above" may include both "above" and "below" orientations. The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.
As shown in FIGS. 1 to 8, the nucleic acid cleavage amplification apparatus comprises: a pushing device 1, a liquid storage device 2 and a chip 3. A plurality of chambers are arranged in the liquid storage device 2, so that the pushing device 1 stretches into the liquid storage device 2, and the lower part of the pushing device 1 is in sliding connection with the liquid storage device 2. One end of the liquid storage device 2 is connected with the upper part of the pushing device 1 in a buckling way, and the other end of the liquid storage device 2 is connected with the chip 1 in a buckling way. The liquid storage device 2 is communicated with the chip 3 through the liquid inlet channel 4.
The chip 3 includes: the chip body 31, the quantitative tank 32, the waste liquid tank 33 and the positioning support column 34, the quantitative tank 32 and the waste liquid tank 33 are provided with a plurality of, the quantitative tank 32 and the waste liquid tank 33 are arranged on the chip body 31, the quantitative tank 32 and the waste liquid tank 33 are respectively communicated with the liquid storage device 2 through the quantitative flow channel 5 and the waste liquid flow channel 6, and the positioning support column 34 is arranged on one side of the bottom of the chip body 31;
the chip 3 further comprises: and one end of the buffer flow channel 7 is communicated with the quantitative flow channel 5, the other end of the buffer flow channel 7 is communicated with the quantitative groove 32, and the sectional area of the buffer flow channel 7 is smaller than that of the quantitative flow channel 5.
When the nucleic acid cleavage amplification device is used, the liquid storage device 2 is connected with the chip 3 in a buckling manner, then the buckle above the pushing device 1 is pressed down, and the pushing device 1 is pressed down, so that liquid in a plurality of chambers inside the liquid storage device 2 is released simultaneously. After releasing the nucleic acid reagent, the nucleic acid reagent enters the quantitative tank 32 of the chip body 31 through the liquid inlet channel 4 to be quantified, and the surplus reagent enters the waste liquid tank 33. In the liquid feeding process, the liquid reagent is mixed with the freeze-dried reagent pre-buried in the quantitative tank 32. After the liquid is fed, the chip body 31 is heated by the apparatus to amplify the liquid in the quantitative tank 32. The microfluidic chip for detecting nucleic acid is simple to operate and the detection time is reduced. In the heating process, as the sectional area of the buffer runner 7 above the quantitative groove 32 is smaller and is positioned at the bottom, liquid in the runner can be quickly vaporized to form an air column, the quantitative groove 32 and the liquid at the upstream are blocked, and the reagent concentration in the quantitative groove 32 is prevented from being diluted, so that the technical effect of improving the accuracy of nucleic acid detection is achieved.
Meanwhile, the nucleic acid splitting and amplifying device is simple in structure and convenient to operate, and can simultaneously release a plurality of samples to be detected so as to achieve the technical effect that a plurality of samples are detected simultaneously.
When the nucleic acid cleavage amplification device is used for releasing liquid, firstly the liquid storage device 2 is connected with the chip 3 in a buckling manner, then the buckle above the pushing device 1 is extruded, and the pushing device 1 is pressed downwards, so that the liquid in a plurality of chambers inside the liquid storage device 2 is released simultaneously, and the purpose of releasing the liquid to equipment to be detected before detection is achieved.
The nucleic acid splitting and amplifying device is simple in structure and convenient to operate, and can simultaneously release a plurality of samples to be detected so as to achieve the technical effect that a plurality of samples are detected simultaneously.
In a specific embodiment, the sample to be tested is a nucleic acid sample and the specific collection sample is a pharyngeal swab.
In order to facilitate the release of nucleic acid reagents, the pushing device 1 comprises: the device comprises a pressing cover 11, a push rod 12 and a piston 13, wherein the piston 13 is fixedly connected to the tail of the push rod 12, the piston 13 stretches into the liquid storage device 2, and the piston 13 is in sliding connection with the liquid storage device 2, so that one end of the liquid storage device 2 is sealed.
The pressing cover 11 is arranged above the push rod 12, and the pressing cover 11 is in buckling connection with the liquid storage device 2. When releasing nucleic acid reagent, need press the buckle of pressing lid 11 department, then push down pressing lid 11, drive push rod 12 and piston 13 and remove, and then utilize the pneumatic drive's mode to drive liquid into inside the chip 3. The liquid release principle is simple, the liquid above the liquid storage device 2 can be sealed through the piston 13, an additional sealing device is not needed, and the sample detection cost is greatly reduced.
In order to prevent premature release of liquid due to mishandling, the nucleic acid cleavage amplification apparatus further comprises: and the limiting device 8 is arranged between the push rod 22 and the piston 23. In a specific embodiment, the limiting device 8 is detachably connected above the liquid storage device 2.
In order to achieve simultaneous release of liquids from different chambers, the reservoir device 2 comprises: the liquid storage device comprises a liquid storage device body 21, a sample liquid storage cavity 22 and a reaction reagent liquid storage cavity 23, wherein the liquid storage device body 21 is in buckling connection with a chip 3, and the sample liquid storage cavity 22 and the reaction reagent liquid storage cavity 23 are formed in the liquid storage device body 21;
the upper end of the sample liquid storage cavity 22 is sealed by a sealing cover 9, and the lower end is sealed by an aluminum foil 6; the upper end of the reaction reagent reservoir 23 is sealed by the piston 13, and the lower end is sealed by the aluminum foil 10.
Further, the liquid storage device 2 further includes: the reaction reagent capacity-expanding cavity 24, the reaction reagent capacity-expanding cavity 24 is opened in the liquid storage device body 21; one end of the reaction reagent expansion cavity 24 is sealed by the piston 13, and the other end is sealed by the liquid storage device 2. The reagent flash chamber 24 communicates with the sample reservoir 22. When the sample is stored, the sealing cover 9 is opened, the liquid reagent is filled in the sample liquid storage cavity 22, and the collected throat swab is placed in the sample liquid storage cavity 22 for stirring, and then the sealing cover is covered again. When releasing the liquid, the push rod 12 and the piston 13 in the reaction reagent liquid storage cavity 23 and the reaction reagent capacity expansion cavity 24 are used for pneumatic driving, and the liquid in the reaction reagent liquid storage cavity 23 and the liquid in the sample liquid storage cavity 22 are driven into the chip 3, so that the detection of the sample is completed.
In order to realize that the liquid in the reaction reagent liquid storage cavity 23 and the sample liquid storage cavity 22 can be driven into the chip 3, the chip 3 is internally provided with a plurality of spike structures 35, and the spike structures 35 are arranged below the sample liquid storage cavity 22 and the reaction reagent liquid storage cavity 23. In the process of releasing the liquid, the aluminum foil 10 below the reaction reagent liquid storage cavity 23 and the sample liquid storage cavity 22 is contacted with the spike structure 35, so that the aluminum foil 10 is extruded and punctured, and the purpose of releasing the liquid is achieved.
In order to facilitate the extraction of the liquid in the quantitative tank 32 and the waste liquid tank 33, the chip 3 further includes: the air holes 36 are formed in a plurality of the air holes 36; the air holes 36 are respectively communicated with the quantitative tank 32 and the waste liquid tank 33 through flow passages.
In order to make the liquid flow into the quantitative tank 32 preferentially, a T-shaped flow passage opening is arranged on the liquid inlet flow passage 4, the quantitative tank 32 and the waste liquid tank 33 are respectively communicated with the T-shaped flow passage opening through the quantitative flow passage 5 and the waste liquid flow passage 6, and the position relationship between the waste liquid flow passage 6 and the liquid inlet flow passage 4 is right angle. The sectional area of the quantitative flow channel 5 is made much larger than that of the waste liquid flow channel 6. The flow resistance of the liquid to the dosing channel 5 is much smaller than the flow resistance to the waste channel 6 so that the liquid preferentially flows to the inside of the dosing channel 32.
In order to facilitate the sealing of the sample liquid storage cavity 22 and the reaction reagent liquid storage cavity 23, the lower ends of the sample liquid storage cavity 22 and the reaction reagent liquid storage cavity 23 are made of plastic materials, and the aluminum foil 10 is bonded at the lower ends of the sample liquid storage cavity 22 and the reaction reagent liquid storage cavity 23 in a hot-pressing mode.
Further, in order to facilitate improvement of the sealing effect of the sample storage chamber 22, the sealing cover 9 is made of plastic material.
The above is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention.
Claims (10)
1. A nucleic acid cleavage amplification device comprising: a pushing device (1), a liquid storage device (2) and a chip (3); the inside of the liquid storage device (2) is provided with a plurality of chambers, the pushing device (1) stretches into the inside of the liquid storage device (2), and the lower part of the pushing device (1) is in sliding connection with the liquid storage device (2); one end of the liquid storage device (2) is connected with the upper part of the pushing device (1) in a buckling manner, and the other end of the liquid storage device (2) is connected with the chip (3) in a buckling manner;
the liquid storage device (2) is communicated with the chip (3) through a liquid inlet flow channel (4);
the chip (3) comprises: chip body (31), ration groove (32), waste liquid groove (33) and location support column (34), ration groove (32) and waste liquid groove (33) are equipped with a plurality ofly, ration groove (32) and waste liquid groove (33) are seted up on chip body (31), ration groove (32) and waste liquid groove (33) are respectively through ration runner (5) and waste liquid runner (6) intercommunication reservoir (2), location support column (34) are established one side of chip body (31) bottom;
the chip (3) further comprises: and one end of the buffer flow channel (7) is communicated with the quantitative flow channel (5), the other end of the buffer flow channel is communicated with the quantitative groove (32), and the sectional area of the buffer flow channel (7) is smaller than that of the quantitative flow channel (5).
2. The nucleic acid cleavage amplification device as recited in claim 1, wherein the pushing device (1) comprises: the device comprises a pressing cover (11), a push rod (12) and a piston (13), wherein the piston (13) is fixedly connected to the tail of the push rod (12), the piston (13) stretches into the liquid storage device (2), and the piston (13) is in sliding connection with the liquid storage device (2) so that one end of the liquid storage device (2) is sealed;
the pressing cover (11) is arranged above the push rod (12), and the pressing cover (11) is in buckling connection with the liquid storage device (2).
3. The nucleic acid cleavage amplification device of claim 2, further comprising: and the limiting device (8) is arranged between the push rod (12) and the piston (13).
4. The nucleic acid cleavage amplification device as recited in claim 1, wherein the liquid storage device (2) comprises: the liquid storage device comprises a liquid storage device body (21), a sample liquid storage cavity (22) and a reaction reagent liquid storage cavity (23), wherein the liquid storage device body (21) is in buckling connection with a chip (3), and the sample liquid storage cavity (22) and the reaction reagent liquid storage cavity (23) are formed in the liquid storage device body (21);
the upper end of the sample liquid storage cavity (22) is sealed by a sealing cover (9), and the lower end is sealed by an aluminum foil (10); the upper end of the reactant liquid storage cavity (23) is sealed by a piston (13), and the lower end is sealed by an aluminum foil (10).
5. The nucleic acid cleavage amplification device as recited in claim 4, wherein the liquid storage device (2) further comprises: a reaction reagent expansion cavity (24), wherein the reaction reagent expansion cavity (24) is arranged in the liquid storage device body (21); one end of the reaction reagent expansion cavity (24) is sealed by a piston (13), and the other end is sealed by a liquid storage device (2); the reagent expansion chamber (24) is in communication with the sample reservoir (22).
6. The nucleic acid cleavage and amplification device according to claim 4, wherein a plurality of spike structures (35) are provided inside the chip (3), and the spike structures (35) are located below the sample liquid storage chamber (22) and the reaction reagent liquid storage chamber (23).
7. The nucleic acid cleavage amplification device as recited in claim 1, wherein the chip (3) further comprises: the air holes (36) are formed in the plurality of the air holes (36); the air holes (36) are respectively communicated with the quantitative tank (32) and the waste liquid tank (33) through flow passages.
8. The nucleic acid cleavage and amplification device according to claim 1, wherein the liquid inlet channel (4) is provided with a T-shaped channel opening, the quantitative tank (32) and the waste liquid tank (33) are respectively communicated with the T-shaped channel opening through the quantitative channel (5) and the waste liquid channel (6), and the position relationship between the waste liquid channel (6) and the liquid inlet channel (4) is right angle;
the sectional area of the quantitative flow channel (5) is far larger than that of the waste liquid flow channel (6).
9. The nucleic acid cleavage and amplification device according to claim 4, wherein the lower ends of the sample liquid storage cavity (22) and the reaction reagent liquid storage cavity (23) are made of plastic materials, and the aluminum foil (10) is bonded to the lower ends of the sample liquid storage cavity (22) and the reaction reagent liquid storage cavity (23) by hot pressing.
10. The nucleic acid cleavage and amplification device according to claim 4, wherein the sealing cover (9) is made of plastic material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210780642.2A CN117384730A (en) | 2022-07-04 | 2022-07-04 | Nucleic acid cracking and amplifying device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210780642.2A CN117384730A (en) | 2022-07-04 | 2022-07-04 | Nucleic acid cracking and amplifying device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117384730A true CN117384730A (en) | 2024-01-12 |
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ID=89439724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210780642.2A Pending CN117384730A (en) | 2022-07-04 | 2022-07-04 | Nucleic acid cracking and amplifying device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117384730A (en) |
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2022
- 2022-07-04 CN CN202210780642.2A patent/CN117384730A/en active Pending
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