CN117368056B - Method for testing particle size and particle size distribution of azithromycin medicine - Google Patents
Method for testing particle size and particle size distribution of azithromycin medicine Download PDFInfo
- Publication number
- CN117368056B CN117368056B CN202311657422.1A CN202311657422A CN117368056B CN 117368056 B CN117368056 B CN 117368056B CN 202311657422 A CN202311657422 A CN 202311657422A CN 117368056 B CN117368056 B CN 117368056B
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- CN
- China
- Prior art keywords
- azithromycin
- sample
- particle size
- dispersion medium
- xanthan gum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 229960004099 azithromycin Drugs 0.000 title claims abstract description 45
- MQTOSJVFKKJCRP-BICOPXKESA-N azithromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)N(C)C[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 MQTOSJVFKKJCRP-BICOPXKESA-N 0.000 title claims abstract description 45
- 239000002245 particle Substances 0.000 title claims abstract description 28
- 239000003814 drug Substances 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000012360 testing method Methods 0.000 title claims abstract description 22
- 239000002612 dispersion medium Substances 0.000 claims abstract description 27
- 229920001285 xanthan gum Polymers 0.000 claims abstract description 20
- 239000000230 xanthan gum Substances 0.000 claims abstract description 19
- 229940082509 xanthan gum Drugs 0.000 claims abstract description 19
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 19
- 229940079593 drug Drugs 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 239000002609 medium Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 15
- 238000010998 test method Methods 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- VQEMDSRIOVZAOM-UHFFFAOYSA-N 4-(4-methylsulfonylphenyl)-1,3-thiazol-2-amine Chemical compound C1=CC(S(=O)(=O)C)=CC=C1C1=CSC(N)=N1 VQEMDSRIOVZAOM-UHFFFAOYSA-N 0.000 claims description 2
- 241000167854 Bourreria succulenta Species 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 claims description 2
- 240000008790 Musa x paradisiaca Species 0.000 claims description 2
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 229960004924 azithromycin dihydrate Drugs 0.000 claims description 2
- 235000019693 cherries Nutrition 0.000 claims description 2
- 239000001863 hydroxypropyl cellulose Substances 0.000 claims description 2
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 claims description 2
- 239000001488 sodium phosphate Substances 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 2
- 229910000406 trisodium phosphate Inorganic materials 0.000 claims description 2
- 235000019801 trisodium phosphate Nutrition 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 11
- 238000003908 quality control method Methods 0.000 abstract description 3
- 238000012827 research and development Methods 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000000463 material Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940088679 drug related substance Drugs 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The invention provides a method for testing the particle size and the particle size distribution of an azithromycin drug, which relates to the technical field of analysis and detection, and specifically comprises the following steps: sample preparation: adding a pre-dispersion medium into a sample to be measured, and uniformly dispersing by vortex; obtaining a test sample; adding a redispersing medium into the sample, stirring and dispersing uniformly, and measuring; the sample to be tested comprises an azithromycin bulk drug and/or an azithromycin dry suspension; the pre-dispersion medium comprises an aqueous xanthan gum solution; the redispersion medium comprises water; the azithromycin medicine comprises azithromycin bulk drug or azithromycin dry suspension. The method has good repeatability and intermediate precision, can simply, conveniently, accurately and efficiently reflect the granularity condition of the azithromycin, and has important significance for research and development of the azithromycin and related preparations, quality control and even clinical application.
Description
Technical Field
The invention relates to the technical field of analysis and detection, in particular to a method for testing the particle size and particle size distribution of an azithromycin medicine.
Background
The insoluble medicine has low solubility in water, unstable absorption in human body and low bioavailability, and the particle size needs to be controlled to ensure the absorption of the medicine in human body. The particle size detection method must pass through method verification, and meets the requirements of specificity, precision, accuracy and other method verification, so that the reliability of impurity detection results can be ensured, and the method can be used for guiding the quality control of medicines.
The particle size detection method generally selects wet measurement or dry measurement according to the properties and solubility of the sample; at present, only dry measurement of granularity of azithromycin is reported in related researches, for example, patent CN109115661A discloses a method for measuring granularity and granularity distribution of azithromycin bulk drug, and a laser granularity dry measurement mode is adopted, wherein measurement parameters comprise background measurement time, sample measurement time, shading rate, sample injection rate and dispersion air pressure. The detection method has high precision, strong accuracy and convenient operation, and can effectively detect the particle size and the particle size distribution of the azithromycin bulk drug. However, the original preparation contains a large amount of auxiliary materials, so that the method cannot analyze the original preparation. At present, no report on the granularity of the azithromycin bulk drug and the azithromycin preparation by a wet method exists.
Aiming at the defects of the related preparation particle size and particle size distribution of the azithromycin in the prior art and the problem that the azithromycin original development agent is difficult to detect by a dry method, the method for testing the particle size and the particle size distribution of the azithromycin bulk drug is very necessary, wherein the method is simple, convenient and accurate and can efficiently reflect the particle size condition of the azithromycin.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a method for testing the particle size and the particle size distribution of an azithromycin drug, which can meet the detection of the particle size of the azithromycin in the azithromycin bulk drug and the azithromycin dry suspension preparation, and has important significance for research and development, quality control and even clinical application of the azithromycin and the azithromycin dry suspension preparation.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the invention provides a method for testing the particle size and the particle size distribution of an azithromycin drug, which comprises the following steps:
(1) Sample preparation: adding a pre-dispersion medium into a sample to be measured, and uniformly dispersing by vortex; obtaining a test sample;
(2) Adding a redispersing medium into the sample, stirring and dispersing uniformly, and measuring;
the sample to be tested comprises an azithromycin bulk drug and/or an azithromycin dry suspension; the pre-dispersion medium comprises an aqueous xanthan gum solution; the redispersion medium comprises water; the azithromycin medicine comprises azithromycin bulk drug or azithromycin dry suspension.
Further, the weight-to-volume ratio of the sample to be measured and the pre-dispersion medium is (0.1-0.25): 5 g/mL. Preferably, the weight to volume ratio of the sample to be measured and the pre-dispersion medium is 0.1:5 g/mL.
Further, the mass volume concentration of the xanthan gum aqueous solution is 0.1-0.3 g/100mL. Preferably, the aqueous xanthan gum solution has a mass to volume concentration of 0.1 g/100mL.
Further, the speed of the vortex is 3000 rpm, and the time is more than or equal to 30s.
Further, the shade is 5-9%.
Further, the stirring speed is 2200 rpm, and the stirring time is more than or equal to 30 and s.
Further, the weight ratio of the redispersion medium to the test sample is 3-4:200.
Further, the limits of the measurement are specifically: d (0.1) is less than 40.0 mu m, d (0.5) is less than or equal to 60 mu m and less than or equal to 160 mu m, d (0.9) is less than or equal to 200 mu m and less than or equal to 300 mu m; taking d (0.1) < 40.0 μm as an example, d (0.1) < 40.0 μm represents that the particle size of the sample to be measured is 10% or less below 40 μm.
The invention has the technical effects that:
the invention uses the characteristic that azithromycin is dissolved under acidic condition and insoluble under neutral and alkaline conditions, a wet method is selected, the granularity of the azithromycin is measured by taking water as a pre-dispersion medium in the development process of the method, the pre-dispersion medium is optimized according to the test repeatability result, and 0.1-0.3% of xanthan gum is taken as the pre-dispersion medium, so that the granularity detection method with good repeatability is obtained.
Detailed Description
Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present invention with reference to specific examples. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention.
Before the embodiments of the invention are explained in further detail, it is to be understood that the invention is not limited in its scope to the particular embodiments described below; it is also to be understood that the terminology used in the examples of the invention is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the invention.
Where numerical ranges are provided in the examples, it is understood that unless otherwise stated herein, both endpoints of each numerical range and any number between the two endpoints are significant both in the numerical range. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It should be noted that the raw materials used in the present invention are all common commercial products, and therefore the sources thereof are not particularly limited.
The instrument and the reagent used in the invention specifically comprise:
instrument: a particle size analyzer, an electronic balance, and a vortex analyzer;
reagent: xanthan gum; the concentration of the xanthan gum solution is g/mL, and taking a 0.1% xanthan gum solution as an example, the mass volume concentration of the xanthan gum aqueous solution is 0.1 g/100mL.
Sample: azithromycin bulk drug, blank auxiliary material and Azithromycin dry suspension, purchased from the company of the sciences pharmaceutical company (hereinafter referred to as formulation, the specific raw materials comprise azithromycin dihydrate, sucrose, anhydrous trisodium phosphate, xanthan gum, hydroxypropyl cellulose, cherry powder essence, banana powder essence and vanilla powder essence).
Example 1
Table 1 test methods and parameters
The particle size is not significantly different when the shading degree is within the range of 5% -9%.
Table 2 test results
Note that: the light shielding degree of the blank auxiliary materials added into the sample cell after pre-dispersing is about 0.2%, and the light shielding degree is consistent with that of the dispersion medium, and cannot be measured.
Example 2
Table 3 test methods and parameters
Table 4 test results
Example 3
Table 5 test methods and parameters
TABLE 6 test results
Comparative example 1
The only difference from example 1 is that the pre-dispersion medium is replaced by an equal volume of water, corresponding to a 1-fold reduction in the concentration of the raw material sample. The same test sample was tested twice.
TABLE 7 test results
From the above table, water is taken as a pre-dispersion medium, the difference of the two measurement results of the same sample is large, the reproducibility is poor, the suspension of the sample is unstable, and the bulk drug is deposited at the bottom more quickly.
Comparative example 2
The only difference from example 1 is that the pre-dispersion medium is replaced by an equal volume of 0.5% xanthan solution.
Comparative example 3
The only difference from example 1 is that the redispersion medium is replaced by an equal volume of 0.1% xanthan gum solution.
Comparative example 4
The difference from example 1 is that the pre-dispersion medium is replaced with an equal volume of 0.1% tween 20 solution.
1. Repeatability of
Samples of each example (each using azithromycin drug) were taken, 6 samples were prepared in parallel for measurement, and RSD (n=6) was calculated to give the following table.
Table 8 example 1 repeatability test results and discussion
TABLE 9 repeatability test results for examples 2-3 and comparative examples 1-4
2. Intermediate precision
The same batch of samples (azithromycin drug substance is selected) was used, the influence on the test results was examined by different operators and different dates, 6 samples were prepared in parallel for measurement, RSD (n=6) was calculated, and RSD (n=12) was calculated for a total of 12 samples. The following table is obtained:
TABLE 10 intermediate precision results and conclusions for example 1
TABLE 11 intermediate precision results for examples 2-3 and comparative examples 1-4
In conclusion, the method for detecting the granularity has the advantages that the blank auxiliary materials have no interference to the granularity detection, the specificity is good, the reproducibility of granularity results of raw materials and preparations is good, the accuracy is high, and the requirement of the granularity detection of samples is met.
The same solvent water was used for the pre-dispersion medium and the re-dispersion medium in comparative example 1, and the same solvent 0.1% xanthan gum solution was used for the pre-dispersion medium and the re-dispersion medium in comparative example 3, so that the repeatability and intermediate precision effects were significantly lower than those of example 1, indicating that different solvents were necessary for the pre-dispersion medium and the re-dispersion medium to have better repeatability and intermediate precision effects.
In comparative example 2, the concentration of the xanthan gum solution is changed, the effect of repeatability and intermediate precision is obviously reduced, which indicates that the concentration of the xanthan gum solution has a great influence on the detection result, and the effect of repeatability and intermediate precision can be better only within a specific range.
The composition of the pre-dispersion medium is changed in comparative example 4, and as a result, the repeatability and the intermediate precision effects are obviously reduced, which means that not all the pre-dispersion medium can obtain good effects for detecting the granularity of azithromycin and related preparations, and the better repeatability and intermediate precision effects can be achieved only when a xanthan gum solution is used as the pre-dispersion medium.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention, and that the simple modification and equivalent substitution of the technical solution of the present invention can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present invention.
Claims (8)
1. A method for testing the particle size and the particle size distribution of an azithromycin medicine is characterized by comprising the following steps: the method comprises the following steps:
(1) Sample preparation: adding a pre-dispersion medium into a sample to be measured, and uniformly dispersing by vortex; obtaining a test sample;
(2) Adding a redispersing medium into the sample, stirring and dispersing uniformly, and measuring;
the sample to be tested comprises an azithromycin bulk drug and/or an azithromycin dry suspension; the pre-dispersion medium comprises an aqueous xanthan gum solution; the redispersion medium comprises water; the azithromycin medicine comprises azithromycin bulk drug or azithromycin dry suspension;
the mass volume concentration of the xanthan gum aqueous solution is 0.1-0.3 g/100mL.
2. The test method according to claim 1, wherein: the weight volume ratio of the sample to be measured and the pre-dispersion medium is (0.1-0.25): 5 g/mL.
3. The test method according to claim 2, wherein: the weight-to-volume ratio of the sample to be measured to the pre-dispersion medium is 0.1:5 g/mL.
4. The test method according to claim 1, wherein: the mass volume concentration of the xanthan gum aqueous solution is 0.1 g/100mL.
5. The test method according to claim 1, wherein: the speed of the vortex is 3000 rpm, and the time is more than or equal to 30 and s.
6. The test method according to claim 1, wherein: the stirring speed is 2200 rpm, and the stirring time is more than or equal to 30 and s.
7. The test method according to claim 1, wherein: the volume ratio of the redispersion medium to the test sample is 3-4:200.
8. The test method according to claim 1, wherein: the dry suspension comprises azithromycin dihydrate, sucrose, anhydrous trisodium phosphate, xanthan gum, hydroxypropyl cellulose, cherry powder essence, banana powder essence and vanilla powder essence.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311657422.1A CN117368056B (en) | 2023-12-06 | 2023-12-06 | Method for testing particle size and particle size distribution of azithromycin medicine |
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| CN202311657422.1A CN117368056B (en) | 2023-12-06 | 2023-12-06 | Method for testing particle size and particle size distribution of azithromycin medicine |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1813683A (en) * | 2005-12-07 | 2006-08-09 | 范敏华 | Azithromycin for suspension and its preparing method |
| CN101836626A (en) * | 2010-05-17 | 2010-09-22 | 天津师范大学 | Fluazinam-containing pesticide suspension concentrate and preparation method thereof |
| CN101856017A (en) * | 2010-05-17 | 2010-10-13 | 天津师范大学 | Suspension concentrate containing difenoconazole and preparation method thereof |
| CN109115661A (en) * | 2018-09-27 | 2019-01-01 | 湖北省宏源药业科技股份有限公司 | A method of for measuring azithromycin drug partial size and size distribution |
| CN110044780A (en) * | 2019-05-05 | 2019-07-23 | 深圳万乐药业有限公司 | A kind of test method of Linezolid bulk pharmaceutical chemicals partial size and size distribution |
| CN114699386A (en) * | 2022-04-14 | 2022-07-05 | 深圳职业技术学院 | Azithromycin composition and preparation method thereof |
| CN116930013A (en) * | 2023-07-28 | 2023-10-24 | 海南卫康制药(潜山)有限公司 | Method for measuring granularity of raw materials in azithromycin dry suspension |
-
2023
- 2023-12-06 CN CN202311657422.1A patent/CN117368056B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1813683A (en) * | 2005-12-07 | 2006-08-09 | 范敏华 | Azithromycin for suspension and its preparing method |
| CN101836626A (en) * | 2010-05-17 | 2010-09-22 | 天津师范大学 | Fluazinam-containing pesticide suspension concentrate and preparation method thereof |
| CN101856017A (en) * | 2010-05-17 | 2010-10-13 | 天津师范大学 | Suspension concentrate containing difenoconazole and preparation method thereof |
| CN109115661A (en) * | 2018-09-27 | 2019-01-01 | 湖北省宏源药业科技股份有限公司 | A method of for measuring azithromycin drug partial size and size distribution |
| CN110044780A (en) * | 2019-05-05 | 2019-07-23 | 深圳万乐药业有限公司 | A kind of test method of Linezolid bulk pharmaceutical chemicals partial size and size distribution |
| CN114699386A (en) * | 2022-04-14 | 2022-07-05 | 深圳职业技术学院 | Azithromycin composition and preparation method thereof |
| CN116930013A (en) * | 2023-07-28 | 2023-10-24 | 海南卫康制药(潜山)有限公司 | Method for measuring granularity of raw materials in azithromycin dry suspension |
Non-Patent Citations (1)
| Title |
|---|
| Improving dissolution and photostability of resveratrol using redispersible dry emulsion: Application of design space for optimizing formulation and spray-drying process;Pontip Benjasirimongkol;《Journal of Drug Delivery Science and Technology》;20191231;全文 * |
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