CN117343835A - Tubular continuous submicron channel microbubble bioreactor - Google Patents
Tubular continuous submicron channel microbubble bioreactor Download PDFInfo
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- CN117343835A CN117343835A CN202311476402.4A CN202311476402A CN117343835A CN 117343835 A CN117343835 A CN 117343835A CN 202311476402 A CN202311476402 A CN 202311476402A CN 117343835 A CN117343835 A CN 117343835A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/20—Mixing gases with liquids
- B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
- B01F23/237—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids characterised by the physical or chemical properties of gases or vapours introduced in the liquid media
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/20—Jet mixers, i.e. mixers using high-speed fluid streams
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/40—Static mixers
- B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
- B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/80—Mixing plants; Combinations of mixers
- B01F33/82—Combinations of dissimilar mixers
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
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Abstract
The invention provides a tubular continuous submicron channel microbubble bioreactor, which comprises a control tank, a feed liquid circulating peristaltic pump, a microbubble generator, a fluid oscillator and a submicron channel static mixer which are sequentially connected, wherein the microbubble generator comprises a rotary impeller, the rotary impeller comprises an impeller cover and an impeller main body, the impeller main body comprises an integrally formed microbubble generating plate and an annular side plate, the upper end surface of the microbubble generating plate is provided with a gas-liquid flow channel, the peripheral side of the gas-liquid flow channel is provided with a packing groove, the packing groove is communicated with the gas-liquid flow channel, the annular side plate is hooped on the microbubble generating plate, a plurality of through holes are uniformly distributed on the annular side plate, and the through holes are communicated with the packing groove; the impeller cover and the impeller main body are connected through bolts, the middle part of the impeller cover is upwards protruded to form an annular boss, and the middle part of the annular boss is provided with a gas or steam inlet. The bioreactor provided by the invention has the advantages of small volume, low manufacturing cost and high safety performance.
Description
Technical Field
The invention belongs to the technical field of chemical industry, and particularly relates to a tubular continuous submicron channel microbubble bioreactor.
Background
Biological products are increasingly widely applied in the fields of common people living, medicine, materials, military industry, communication, energy sources and the like, so to speak, the biological products are directly related to national security, however, a biological reactor is core equipment for producing biological products; whether the bioreactor is advanced or not directly influences the quality and cost of the product, and also influences the popularization and application of the product. Currently, bioreactors are stirred, airlift, self-priming and bubbling, mostly interstitial, large-sized, and belong to pressure vessels; thus, current bioreactors are not only expensive to manufacture but also have a relatively high risk of operating the apparatus. How to make the bioreactor equipment continuous and miniaturized, which can be influenced by the unique properties of different microorganisms, but the most important factors can be influenced by mass and heat transfer of the bioreactor; in particular to the influence of multiphase mass transfer and heat transfer of gas-liquid, gas-liquid-solid and the like.
For most gas-liquid mass transfer reactions, the mass transfer process runs through the whole mass transfer-reaction control process, the main resistance of gas-liquid mass transfer comes from the liquid film and the internal pressure of the bubbles, if the diameter of the bubbles is small, the internal pressure is high, the gas-liquid mass transfer rate can be enhanced, and meanwhile, the bubbles with small diameters can not only increase the specific surface area of the gas-liquid reaction, but also prolong the residence time of the bubbles in the solution and prolong the reaction time; in addition, strengthening the liquid phase turbulence will help reduce mass transfer resistance.
The traditional bubbling reactor generally adopts a simple tube type perforated gas distributor, and the gas distributor is easy to generate bubbles with diameters of a few centimeters or even more than ten centimeters, and can be used for a slow reaction system, but liquid in the reaction system is seriously mixed back, the bubbles are easy to generate coalescence, and the reaction efficiency is low. For a slow reaction system, the chemical reaction rate is lower than the diffusion rate, and the chemical reaction is mainly carried out in a liquid phase for kinetic control.
Meanwhile, as the existing bioreactor system is large, mass transfer and heat transfer are mainly realized through mechanical stirring or gas stirring, so that not only is a great deal of energy consumption required, but also the back mixing of the reaction system is caused to influence the gas-liquid-solid mass transfer, and the production cost is increased.
Therefore, there is an urgent need for a bioreactor that can solve both the mass and heat transfer of the current bioreactor and reduce the manufacturing cost of the production equipment and the production cost of the product. The present application proposes a continuous, miniaturized device that solves this problem.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a tubular continuous submicron channel microbubble bioreactor which can not only continuously react but also intermittently react, and the microbubble bioreactor also utilizes a rotating impeller with a novel structure to prepare microbubbles, and simultaneously, the reactor also utilizes a fluid oscillator and a submicron pipeline static mixer to strengthen fluid mixing and prevent the fluid from backmixing so as to prevent bubble coalescence, improve mass transfer and heat transfer efficiency and reduce production cost; the invention has small volume, low manufacturing cost and high safety performance. The reactor has the characteristics of good gas-liquid mixing effect, simple structure, convenient maintenance, flexible operation and the like.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the tubular continuous submicron channel microbubble bioreactor comprises a control tank, a feed liquid circulating peristaltic pump, a microbubble generator, a fluid oscillator and a submicron pipeline static mixer which are sequentially connected in a closed loop along the feed liquid flowing direction, wherein a tail gas outlet, a control tank feed inlet, a control tank discharge outlet and a control tank feed liquid circulating port are arranged on the control tank; the micro-bubble generator is connected with a gas or steam feeding pipe and comprises a rotary impeller, the rotary impeller comprises an impeller cover and an impeller main body, the impeller main body comprises an integrally formed micro-bubble generation plate and an annular side plate, a gas-liquid flow passage is arranged on the upper end face of the micro-bubble generation plate, a packing groove is arranged on the peripheral side of the gas-liquid flow passage and communicated with the gas-liquid flow passage, the annular side plate is hooped on the micro-bubble generation plate, a plurality of through holes are uniformly distributed on the annular side plate, and the through holes are communicated with the packing groove; the impeller cover and the impeller main body are connected through bolts, the middle part of the impeller cover is upwards protruded to form an annular boss, and the middle part of the annular boss is provided with a gas or steam inlet.
Further, the gas-liquid runner is separated by a plurality of bulkheads on the vertical microbubble generating plate that are equipped with and forms, and a plurality of the bulkheads include a plurality of first arc bulkheads and a plurality of second arc bulkheads, first arc bulkheads and second arc bulkheads set up along circumference in turn, and the one end of first arc bulkheads and the lateral wall fixed connection of annular boss, the one end that the second arc bulkheads is close to annular boss and the lateral wall interval setting of annular boss, and the one end that annular boss was kept away from to first arc bulkheads and second arc bulkheads is located the outer fringe of same circle, forms gas-liquid forced mixing and throws away the runner between second arc bulkheads and the first arc bulkheads.
Further, the gas-liquid flow channel is formed by separating a plurality of partition walls vertically arranged on the microbubble generating plate, the plurality of partition walls comprise a plurality of first rectangular partition walls, the plurality of first rectangular partition walls are uniformly distributed on the circumferential side of the annular boss along the circumferential direction, and one end of the first rectangular partition wall, which is close to the annular boss, is arranged at intervals with the annular boss; a first diamond-shaped partition wall and a second rectangular partition wall are sequentially arranged between two adjacent first rectangular partition walls at intervals along the mirror image from inside to outside, two sides of the first diamond-shaped partition wall are arranged at intervals with the two first rectangular partition walls, and the central axes of the first diamond-shaped partition wall and the second rectangular partition wall along the radial direction are overlapped with the symmetrical lines of the two first rectangular partition walls; two second diamond-shaped partition walls are symmetrically arranged on two second rectangular partition walls, and the left side and the right side of each second diamond-shaped partition wall are respectively arranged at intervals with the first rectangular partition wall and the second rectangular partition wall; one ends of the first rectangular partition wall, the second rectangular partition wall and the second diamond-shaped partition wall, which are far away from the annular boss, are positioned at the outer edge of the same circle, and a gas-liquid forced mixing and throwing-out flow passage is formed between the second arc-shaped partition wall and the first arc-shaped partition wall.
Further, the microbubble generator further comprises a barrel, a magnetic fluid sealing piece, a motor and an impeller cover, wherein the impeller cover and the rotary impeller are arranged inside the barrel, the impeller cover is hung on the inner side wall of a top plate of the barrel, an opening is formed in the top plate of the impeller cover, the rotary impeller is arranged in the impeller cover, an annular boss of the rotary impeller is arranged in the opening, a mounting opening is formed in the top plate of the barrel, a microbubble generator feed liquid outlet and a microbubble generator feed liquid inlet are formed in the side wall, the magnetic fluid sealing piece is arranged on the upper end face of the barrel, the upper end of a magnetic conduction transmission shaft in the magnetic fluid sealing piece is connected with an output shaft of the motor, a feed channel is formed inside the magnetic conduction transmission shaft along the length direction of the magnetic conduction transmission shaft, a gas or steam runner which is perpendicular to the feed channel is arranged on the magnetic fluid sealing piece, and a lower port of the feed channel extends into the barrel from the mounting opening to be in butt joint with the feed port of the rotary impeller.
Further, the impeller cover comprises an annular top plate, an annular supporting plate and an annular bottom plate which are sequentially arranged at intervals from bottom to top, a plurality of supporting columns are uniformly distributed between the annular bottom plate and the annular supporting plate, and a plurality of guide plates which are arranged along the radial direction are connected between the annular supporting plate and the annular bottom plate; the annular boss is arranged on the annular top plate, and an opening in the middle of the annular bottom plate is a feed liquid inlet; a plurality of fixing bolt holes are uniformly distributed on the annular top plate, and the impeller cover is connected with the cylinder body through bolts penetrating through the fixing bolt holes.
Further, the inner cavity of the fluid oscillator comprises an inlet convergent section, a diffusion cavity, a flow guide boss and an outlet diffusion section, the feed liquid outlet of the microbubble generator is communicated with the inlet convergent section, the inlet convergent section is connected with the diffusion cavity, the flow guide boss is arranged in the diffusion cavity, the diffusion cavity is connected with the outlet diffusion section, and the outlet diffusion section is communicated with the feed inlet of the submicron pipeline static mixer.
Further, the sub-microchannel static mixer comprises a plurality of static mixer pipe bodies, wherein reinforced mixing components are arranged in the static mixer pipe bodies, heat exchange jackets are sleeved outside the static mixer pipe bodies, the outlet end of the former static mixer pipe body and the inlet end of the latter static mixer pipe body are connected through a U-shaped bent pipe along the flowing direction of liquid, the heat exchange jackets on the two adjacent static mixer pipe bodies are communicated through pipelines, a steam condensate outlet and a cooling water inlet are arranged on the heat exchange jackets close to the fluid oscillator, and a steam inlet and a cooling water outlet are arranged on the heat exchange jackets close to the control tank.
Further, the reinforced mixing component is one or more of 180-degree or 270-degree positive and negative spiral sheets, spiral sheets with the orientation of double pore channels of adjacent units staggered by 90 degrees and length units formed by intersecting transverse strips, and the transverse strips form 45 degrees with the axis of the pipe shell.
Further, a plurality of static mixer pipe bodies and the U-shaped bent pipe are connected through one or more of a flange, a clamping sleeve and a clamping hoop.
Further, a feed supplement port and a control tank sight glass are arranged on the control tank, and a feed liquid circulation port of the control tank is arranged along the tangential direction of the tank body of the control tank; the diameter of the control tank is 250mm, the height of the control tank is 500mm, the height-diameter ratio reaches 2:1, the diameter of the tank body of the microbubble generator is 200mm, and the height of the tank body of the microbubble generator is 200-300 mm; the impeller rotating speed of the microbubble generator is 1000-3000 rpm, preferably 2000-2500 rpm; the filler is one or more of a wire mesh, ceramic particles and a small Raschig ring.
The micro-bubble generator can generate micro-scale bubbles, and has simple structure and low cost; meanwhile, the method is not limited to a bioreactor, and can be used for the reaction of a general gas-liquid two-phase and gas-liquid-solid three-phase system.
The invention utilizes the heat exchange jacket to control the sterilization or temperature of the system, and can well control the temperature of the system due to the large heat exchange specific surface area of the submicron pipeline. During sterilization, steam enters the static mixer jacket from above the static mixer and then flows down the static mixer in sequence, and steam condensate flows out of the lowest static mixer. After sterilization, the cooling water enters from the lowermost static mixer, goes up the static mixer in sequence, and then exits from the uppermost static mixer. The temperature control process is that hot water for heating or cooling water for cooling flows in from the bottom static mixer jacket, and flows up from the top static mixer jacket sequentially along the static mixer jacket.
Compared with the prior art, the invention has the following beneficial effects:
1. the bioreactor has larger production capacity and higher safety due to the design of continuous and miniaturized equipment;
2. the invention adopts a special structure to prepare the micro-bubbles, which has the advantages of energy saving, blockage prevention, gas-liquid contact area improvement and mass transfer efficiency great improvement;
3. the invention can prevent the fluid from backmixing in a large area by utilizing the structures of the fluid oscillator and the static mixer of the sub-micro pipeline, improves the mass transfer efficiency of the system, and simultaneously greatly improves the heat exchange effect due to the large heat transfer area of the sub-micro pipeline;
4. the tangential design of the circulating reflux pipe of the control tank is beneficial to gas-liquid separation after reaction and discharges the fermented harmful gas out of the system as soon as possible;
5. the quick-opening structure is beneficial to disassembling and cleaning of the device and ensures the quality of microorganism culture;
6. the gas-liquid reactor has simple structure and low cost;
7. the gas-liquid reactor has wide applicability, is not limited to a bioreactor, and can be used for the reaction of a general gas-liquid two-phase and gas-liquid-solid three-phase system.
Drawings
FIG. 1 is a schematic structural diagram of a tubular continuous submicron channel microbubble bioreactor according to the present invention.
Fig. 2 is a schematic structural diagram of a microbubble generator according to the present invention.
Fig. 3 is a schematic diagram of an assembled structure of the rotary impeller according to the present invention.
Fig. 4 is a schematic structural view of a microbubble generation board according to embodiment 1 of the present invention.
Fig. 5 is a schematic structural view of a microbubble generation board according to embodiment 2 of the present invention.
Fig. 6 is a schematic structural diagram of a fluidic oscillator according to the present invention.
Fig. 7 is a schematic structural view of the impeller housing according to the present invention.
FIG. 8 is a flow chart of the sub-micron static mixer jacket sterilization, cooling and temperature control process according to the present invention.
Wherein, 1-control the tank; 2-a feed liquid circulating peristaltic pump; 3-a microbubble generator; a 4-fluidic oscillator; a 5-sub-microchannel static mixer; 6-sampling and discharging the materials by a system; 11-a tail gas outlet; 12-a feed supplement port; 13-controlling a feed inlet of the tank; 14-controlling the can sight glass; 15-controlling a circulating discharge port of the tank; 16-controlling a tank feed liquid circulation port; 31-a magnetic fluid seal; 32-motor, 33-gas or vapor feed pipe, 34-microbubble generator feed liquid outlet, 35-impeller housing, 36-rotating impeller, 37-cylinder, 38-microbubble generator feed liquid inlet, 311-gas or vapor flow channel, 312-feed channel, 361-impeller cover, 362-microbubble generation plate, 363-annular side plate, 362-1-packing slot, 361-1-bolt hole, 361-2-annular boss, 364-first arched partition wall, 365-second arched partition wall, 366-first rectangular partition wall, 367-first diamond partition wall, 368-second rectangular partition wall, 369-second diamond partition wall, 351-annular top plate, 352-annular support plate, 353-annular bottom plate, 354-support column, 355-baffle, 353-1-feed liquid inlet, 351-1-fixed bolt hole, 41-inlet tapered section, 42-diffusion chamber, 43-guide boss, 44-outlet diffusion section, 51-static mixer tube, 52-intensified mixing element, 53-heat exchange jacket, 54-U-type elbow, 55-56-steam outlet and cooling water inlet, 57-cooling water inlet.
Description of the embodiments
The invention will be further described with reference to the accompanying drawings and specific examples.
Examples
The tubular continuous submicron channel microbubble bioreactor as shown in figures 1-4 and 6-8 comprises a control tank 1, a feed liquid circulating peristaltic pump 2, a microbubble generator 3, a fluid oscillator 4 and a submicron pipeline static mixer 5 which are sequentially connected in a closed loop along the feed liquid flowing direction, wherein a tail gas outlet 11, a control tank feed inlet 13, a control tank circulating discharge port 15, a feed supplement port 12, a control tank sight glass 14 and a control tank feed liquid circulating port 16 are arranged on the control tank 1, the feed liquid circulating port of the control tank 1 is arranged along the tangential direction of the tank body of the control tank 1, the control tank circulating discharge port 15 is connected with the feed inlet of the feed liquid circulating peristaltic pump 2 through a pipeline, the discharge port of the submicron pipeline static mixer is communicated with the control tank feed liquid circulating port 16, and a system sampling and discharge port 6 is arranged on the pipeline between the submicron pipeline static mixer 5 and the control tank 1; the micro-bubble generator 3 is connected with a gas or steam feed pipe 33, the micro-bubble generator 3 comprises a cylinder 37, a magnetic fluid sealing piece 31, a motor 32, an impeller cover 35 and a rotary impeller 36, the impeller cover 35 and the rotary impeller 36 are arranged in the cylinder 37, the impeller cover 35 is hung on the inner side wall of the top plate of the cylinder 37, an opening is arranged on the top plate of the impeller cover 35, a feed port of the rotary impeller 36 is clamped in the opening, a mounting port is arranged on the top plate of the cylinder 37, a micro-bubble generator feed liquid outlet 34 and a micro-bubble generator feed liquid inlet 38 are arranged on the side wall of the cylinder 37, the micro-bubble generator feed liquid outlet 34 is connected with the feed port of the fluid oscillator 4, the micro-bubble generator feed liquid inlet 38 is communicated with the discharge port of the feed liquid circulating peristaltic pump 2, the magnetic fluid sealing piece 31 is arranged on the upper end surface of the cylinder 37, the upper end of a magnetic transmission shaft in the magnetic fluid sealing piece 31 is connected with the output shaft of the motor 32, a feed channel 312 is arranged in the magnetic transmission shaft along the length direction, a gas or steam flow channel 311 which is vertically intersected with the feed channel 312 is arranged on the magnetic fluid sealing piece 31, the gas or steam channel 311 is communicated with the feed port of the rotary impeller 312, and the feed port is connected with the feed port of the rotary impeller 37; the rotary impeller 36 comprises an impeller cover 361 and an impeller main body, the impeller main body comprises an integrally formed micro-bubble generating plate 362 and an annular side plate 363, a gas-liquid flow passage is arranged on the upper end face of the micro-bubble generating plate 362, a packing groove 362-1 is arranged on the periphery side of the gas-liquid flow passage, the packing groove 362-1 is communicated with the gas-liquid flow passage, packing is filled in the packing groove 362-1, the annular side plate 363 is hooped on the micro-bubble generating plate 362, a plurality of through holes are uniformly distributed on the annular side plate 363, and the through holes are communicated with the packing groove 362-1; the micro-bubble generating plate 362 and the blade cover 361 are oppositely provided with a plurality of bolt holes 361-1, the blade cover 361 and the blade body are connected through bolts, the middle part of the blade cover 361 protrudes upwards to form an annular boss 361-2, and the middle part of the annular boss 361-2 is provided with a gas or steam inlet; the gas-liquid flow channel is formed by separating a plurality of partition walls vertically arranged on the microbubble generating plate 362, the plurality of partition walls comprise a plurality of first arc partition walls 364 and a plurality of second arc partition walls 365, the first arc partition walls 364 and the second arc partition walls 365 are alternately arranged along the circumferential direction, one end of the first arc partition walls 364 is fixedly connected with the outer side wall of the annular boss 361-2, one end of the second arc partition walls 365 close to the annular boss 361-2 is arranged at intervals with the outer side wall of the annular boss 361-2, one ends of the first arc partition walls 364 and the second arc partition walls 365 far away from the annular boss 361-2 are positioned at the outer edge of the same circle, and gas-liquid forced mixing and throwing-out flow channels are formed between the second arc partition walls 365 and the first arc partition walls 364; the filler is one or more of wire mesh, ceramic particles and small Raschig rings.
The impeller cover 35 comprises an annular top plate 351, an annular support plate 352 and an annular bottom plate 353 which are sequentially arranged at intervals from bottom to top, a plurality of support columns 354 are uniformly distributed between the annular bottom plate 353 and the annular support plate 352, and a plurality of guide plates 355 which are arranged along the radial direction are connected between the annular support plate 352 and the annular bottom plate 353; the annular boss 361-2 is arranged on the annular top plate 351, and an opening in the middle of the annular bottom plate 353 is a feed liquid inlet 353-1; a plurality of fixing bolt holes 351-1 are uniformly distributed on the annular top plate 351, and the impeller cover 35 is connected to the cylinder 37 by bolts penetrating the fixing bolt holes 351-1. The inner cavity of the fluid oscillator 4 comprises an inlet tapered section 41, a diffusion cavity 42, a flow guide boss 43 and an outlet diffusion section 44, the feed liquid outlet 34 of the micro bubble generator is communicated with the inlet tapered section 41, the inlet tapered section 41 is connected with the diffusion cavity 42, the flow guide boss 43 is arranged in the diffusion cavity 42, the diffusion cavity 42 is connected with the outlet diffusion section 44, and the outlet diffusion section 44 is communicated with the feed inlet of the submicron pipeline static mixer 5.
The sub-microchannel static mixer 5 comprises a plurality of static mixer pipe bodies 51, wherein reinforced mixing components 52 are arranged inside the static mixer pipe bodies 51, heat exchange jackets 53 are sleeved outside the static mixer pipe bodies 51, the outlet end of the former static mixer pipe body 51 and the inlet end of the latter static mixer pipe body 51 are connected through a U-shaped bent pipe 54 along the liquid flowing direction, the static mixer pipe bodies 51 and the U-shaped bent pipe 54 are connected through a clamp 55, the heat exchange jackets 53 on the two adjacent static mixer pipe bodies 51 are communicated through pipelines, steam condensate water outlets and cooling water inlets 56 are arranged on the heat exchange jackets 53 close to the fluid oscillator, and steam inlets and cooling water outlets 57 are arranged on the heat exchange jackets 53 close to the control tank.
The reinforced mixing component 52 is one or more of 180 degrees or 270 degrees positive and negative spiral sheets, spiral sheets with the orientation of adjacent unit double pore channels staggered by 90 degrees and length units formed by intersecting transverse strips, and the transverse strips form 45 degrees with the axis of the pipe shell.
The diameter of the control tank 1 is 250mm, the height of the control tank is 500mm, the height-diameter ratio reaches 2:1, the diameter of the cylinder body of the microbubble generator 3 is 200mm, the height of the cylinder body of the microbubble generator is 200-300 mm, and the rotating speed of the impeller of the microbubble generator 3 is 1000-3000 rpm, preferably 2000-2500 rpm.
Fresh air of the microbubble generator 3 enters from a gas or steam feeding pipe, passes through a magnetic fluid sealing piece and reaches a generator rotating impeller, the impeller is driven by a motor to rotate at a high speed, and liquid and gas pass through a special flow passage and shear of filler in the impeller to generate microbubbles which enter a reaction system. The micro-bubble mixed liquid enters a fluid oscillator under the pushing of a peristaltic pump, and oscillation jet flow can be generated in the fluid oscillator due to the wall attaching effect, so that the self-oscillation of the fluid is realized, and the mixing can be enhanced; then, the fluid continuously enters a sub-microchannel static mixer and is subjected to gas-liquid-solid mixing enhancement through components of the static mixer; finally, the reaction liquid is circulated back to the control tank or discharged.
Ribonucleic acid (RNA) was prepared by the above apparatus using candida tropicalis: the prepared culture solution enters a control tank from a liquid inlet 13 of the device, a circulating peristaltic pump 2 is started, the culture solution is sterilized firstly, steam enters a jacket from a medium inlet 54 in the jacket of the submicron static mixer to heat the culture solution, steam condensate is discharged through a medium outlet 51 in the jacket, when the temperature of the solution is raised to 100 ℃, the jacket is closed to heat the culture solution, the temperature is continuously raised to 121 ℃ by using steam, sterilization is carried out for 30 minutes, the temperature is reflected on an instrument through a thermometer probe, after sterilization is finished, an air inlet 33 of the microbubble device 3 is opened to enable sterile air to enter the system to keep the pressure in the system at 0.1MPa, a medium inlet 51 in the jacket of the static mixer and a medium outlet 54 in the jacket are opened to cool the culture solution, the temperature of the culture solution is stopped to be reduced at 30 ℃, strains are inoculated for cultivation, and continuous cultivation is carried out under the conditions of 30 ℃ and pH=4.0. 800 g.L is continuously added under the condition of controlling pH=4.0 -1 The concentration of sugar in the system effluent liquid is controlled to be 2 g L -1 The following is given. Sterile air enters from the air inlet device 3, then is prepared into micro bubbles through a micro bubble generator to enter into solution, and finally, mixed gas with complete reaction leaves the reaction device through the air outlet 11. The device can be used for preparing candida tropicalis with biomass of 180. g.L -1 RNA concentration was 11. g g -1 RNA yield was up to 150.09 mg/(L.h).
Examples
As shown in fig. 1-3, 5 and 6-8, the apparatus used in this example is substantially the same as that of embodiment 1, except that in this embodiment, the gas-liquid flow passage of the rotary impeller 36 is divided by a plurality of partition walls provided vertically with a microbubble generating plate, the plurality of partition walls include a plurality of first rectangular partition walls 366, the plurality of first rectangular partition walls 366 are circumferentially and uniformly distributed on the circumferential side of the annular boss 361-2, and one end of the first rectangular partition walls 366 close to the annular boss 361-2 is spaced from the annular boss 361-2; a first diamond-shaped partition wall 367 and a second rectangular partition wall 368 are sequentially arranged between two adjacent first rectangular partition walls 366 at intervals along the mirror image from inside to outside, two sides of the first diamond-shaped partition wall 367 are arranged at intervals with the two first rectangular partition walls 366, and the central axes of the first diamond-shaped partition wall 367 and the second rectangular partition wall 368 along the radial direction are overlapped with the symmetrical lines of the two first rectangular partition walls 366; two second diamond-shaped partition walls 369 are symmetrically arranged on two second rectangular partition walls 368, and the left and right sides of the two second diamond-shaped partition walls 369 are arranged at intervals from the first rectangular partition walls 366 and the second rectangular partition walls 368; the ends of the first rectangular partition wall 366, the second rectangular partition wall 368 and the second diamond partition wall 369, which are far away from the annular boss 361-2, are positioned on the outer edge of the same circle, a plurality of shearing flow passages for forcibly mixing and throwing out the gas and the liquid are formed between the second arc partition wall 365 and the first arc partition wall 364, and other similar structures can be designed.
The lysine hydrochloride is prepared by using corynebacterium glutamicum by adopting the device: the prepared culture solution enters a control tank from a liquid inlet 13 of the device, a circulating peristaltic pump 2 is started, the culture solution is sterilized firstly, steam enters a jacket from a medium inlet 54 in a jacket of a submicron static mixer to heat the culture solution, steam condensate is discharged through a medium outlet 51 in the jacket, when the temperature of the solution rises to 100 ℃, the jacket is closed to heat the culture solution, the temperature of the culture solution is continuously raised to 121 ℃ by using steam, sterilization is carried out for 30 minutes, the temperature is reflected on an instrument by a thermometer probe, after sterilization is finished, an air inlet 33 of a microbubble device 3 is opened to enable sterile air to enter a system to keep the pressure in the system at 0.1MPa, a medium inlet 51 in the jacket of the static mixer and a medium outlet 54 in the jacket are opened, and the culture solution is reducedThe temperature of the culture solution is 30 ℃, the temperature is stopped to be reduced, strains are inoculated for culturing, and the culture is continuously carried out under the conditions of the temperature of 30 ℃ and the pH value of 4.0. 800 g.L is continuously added under the condition of controlling pH=4.0 -1 The concentration of sugar in the system effluent liquid is controlled to be 1 g L -1 The following is given. Sterile air enters from the air inlet device 3, then is prepared into micro bubbles through a micro bubble generator to enter into solution, and finally, mixed gas with complete reaction leaves the reaction device through the air outlet 11. Lysine hydrochloride can be prepared by the device of the invention, and the maximum biomass is 180. g.L -1 Lysine hydrochloride concentration reaches 213.7 g.L -1 。
In the description of the present application, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "inner," "outer," and the like indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, merely to facilitate description of the present application and simplify the description, and do not indicate or imply that the devices or elements being referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.
The foregoing describes specific embodiments of the present invention. It is to be understood that the invention is not limited to the particular embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without affecting the spirit of the invention. The embodiments of the present application and features in the embodiments may be combined with each other arbitrarily without conflict. Those of ordinary skill in the art will appreciate that: the foregoing description of the embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (10)
1. The tubular continuous submicron channel microbubble bioreactor is characterized by comprising a control tank, a feed liquid circulating peristaltic pump, a microbubble generator, a fluid oscillator and a submicron pipeline static mixer which are sequentially connected in a closed loop along the feed liquid flowing direction, wherein a tail gas outlet, a control tank feed inlet, a control tank discharge outlet and a control tank feed liquid circulating port are arranged on the control tank, the discharge outlet of the control tank is connected with the feed inlet of the feed liquid circulating peristaltic pump through a pipeline, the discharge outlet of the submicron pipeline static mixer is communicated with the feed liquid circulating port, and a system sampling and discharge outlet is arranged on the pipeline between the submicron pipeline static mixer and the control tank; the micro-bubble generator is connected with a gas or steam feeding pipe and comprises a rotary impeller, the rotary impeller comprises an impeller cover and an impeller main body, the impeller main body comprises an integrally formed micro-bubble generation plate and an annular side plate, a gas-liquid flow passage is arranged on the upper end face of the micro-bubble generation plate, a packing groove is arranged on the peripheral side of the gas-liquid flow passage and communicated with the gas-liquid flow passage, the annular side plate is hooped on the micro-bubble generation plate, a plurality of through holes are uniformly distributed on the annular side plate, and the through holes are communicated with the packing groove; the impeller cover and the impeller main body are connected through bolts, the middle part of the impeller cover is upwards protruded to form an annular boss, and the middle part of the annular boss is provided with a gas or steam inlet.
2. The continuous submicron channel microbubble bioreactor as set forth in claim 1, characterized in that the gas-liquid flow channel is formed by a plurality of partition walls on a microbubble generating plate, the partition walls comprise a plurality of first arc partition walls and a plurality of second arc partition walls, the first arc partition walls and the second arc partition walls are alternately arranged along the circumferential direction, one end of the first arc partition wall is fixedly connected with the outer side wall of the annular boss, one end of the second arc partition wall close to the annular boss is arranged at intervals with the outer side wall of the annular boss, one ends of the first arc partition walls and the second arc partition walls far away from the annular boss are located at the outer edge of the same circle, and a gas-liquid forced mixing and throwing flow channel is formed between the second arc partition walls and the first arc partition walls.
3. The tubular continuous submicron-channel microbubble bioreactor as set forth in claim 1, characterized in that the gas-liquid flow channel is partitioned by a plurality of partition walls vertically provided with microbubble generating plates, wherein the plurality of partition walls comprise a plurality of first rectangular partition walls which are uniformly distributed on the circumferential side of the annular boss along the circumferential direction, and one end of the first rectangular partition wall, which is close to the annular boss, is arranged at intervals with the annular boss; a first diamond-shaped partition wall and a second rectangular partition wall are sequentially arranged between two adjacent first rectangular partition walls at intervals along the mirror image from inside to outside, two sides of the first diamond-shaped partition wall are arranged at intervals with the two first rectangular partition walls, and the central axes of the first diamond-shaped partition wall and the second rectangular partition wall along the radial direction are overlapped with the symmetrical lines of the two first rectangular partition walls; two second diamond-shaped partition walls are symmetrically arranged on two second rectangular partition walls, and the left side and the right side of each second diamond-shaped partition wall are respectively arranged at intervals with the first rectangular partition wall and the second rectangular partition wall; one ends of the first rectangular partition wall, the second rectangular partition wall and the second diamond-shaped partition wall, which are far away from the annular boss, are positioned at the outer edge of the same circle, and a gas-liquid forced mixing and throwing-out flow passage is formed between the second arc-shaped partition wall and the first arc-shaped partition wall.
4. A tubular continuous submicron channel microbubble bioreactor according to claim 2 or 3, characterized in that the microbubble generator further comprises a cylinder, a magnetic fluid sealing member, a motor and an impeller cover, wherein the impeller cover and the rotating impeller are arranged inside the cylinder, the impeller cover is hung on the inner side wall of a top plate of the cylinder, an opening is arranged on the top plate of the impeller cover, the rotating impeller is arranged in the impeller cover, an annular boss of the rotating impeller is arranged in the opening, a mounting opening is arranged on the top plate of the cylinder, a microbubble generator feed liquid outlet and a microbubble generator feed liquid inlet are arranged on the side wall, the magnetic fluid sealing member is arranged on the upper end face of the cylinder, the upper end of a magnetic transmission shaft in the magnetic fluid sealing member is connected with an output shaft of the motor, a feed channel is arranged inside the magnetic transmission shaft along the length direction of the magnetic transmission shaft, a gas or steam feed pipe which is vertically intersected with the feed channel is arranged on the magnetic fluid sealing member, and the lower port of the feed channel extends into the cylinder from the mounting opening to the inside the cylinder to be in butt joint with the feed port of the rotating impeller.
5. The tubular continuous submicron channel microbubble bioreactor as set forth in claim 4, characterized in that the impeller cover comprises an annular top plate, an annular support plate and an annular bottom plate which are sequentially arranged at intervals from bottom to top, wherein a plurality of support columns are uniformly distributed between the annular bottom plate and the annular support plate, and a plurality of guide plates arranged along the radial direction are connected between the annular support plate and the annular bottom plate; the annular boss is arranged on the annular top plate, and an opening in the middle of the annular bottom plate is a feed liquid inlet; a plurality of fixing bolt holes are uniformly distributed on the annular top plate, and the impeller cover is connected with the cylinder body through bolts penetrating through the fixing bolt holes.
6. The tubular continuous submicron channel microbubble bioreactor of claim 1, characterized in that the inner cavity of the fluid oscillator comprises an inlet tapered section, a diffusion cavity, a flow guiding boss and an outlet diffusion section, the feed liquid outlet of the microbubble generator is communicated with the inlet tapered section, the inlet tapered section is connected with the diffusion cavity, the flow guiding boss is arranged in the diffusion cavity, the diffusion cavity is connected with the outlet diffusion section, and the outlet diffusion section is communicated with the feed inlet of the submicron channel static mixer.
7. The tubular continuous submicron channel microbubble bioreactor as set forth in claim 1, characterized in that the submicron channel static mixer comprises a plurality of static mixer tube bodies, wherein the inside of the static mixer tube bodies is provided with reinforced mixing components, the outside of the static mixer tube bodies is sleeved with heat exchange jackets, the outlet ends of the former static mixer tube bodies and the inlet ends of the latter static mixer tube bodies are connected through U-shaped bent pipes along the flowing direction of the liquid, the heat exchange jackets on the adjacent two static mixer tube bodies are communicated through pipelines, the heat exchange jackets near the fluid oscillator are provided with steam condensate water outlets and cooling water inlets, and the heat exchange jackets near the control tank are provided with steam inlets and cooling water outlets.
8. The tubular continuous submicron-channel microbubble bioreactor according to claim 7, characterized in that the intensified mixing component is one or more of 180 ° or 270 ° positive and negative spiral sheets, spiral sheets with 90 ° dislocation of adjacent unit double-channel orientations, and units composed of intersecting cross bars, the cross bars being 45 ° from the axis of the tube shell.
9. The tubular continuous submicron-channel microbubble bioreactor as set forth in claim 7, characterized in that a plurality of said static mixer tubes and U-shaped elbows are connected by one or more of flanges, ferrules, clips.
10. The tubular continuous submicron channel microbubble bioreactor as set forth in claim 1, characterized in that the control tank is provided with a feed supplement port and a control tank sight glass, and the feed liquid circulation port of the control tank is arranged along the tangential direction of the tank body of the control tank; the diameter of the control tank is 250mm, the height of the control tank is 500mm, the height-diameter ratio reaches 2:1, the diameter of the tank body of the microbubble generator is 200mm, and the height of the tank body of the microbubble generator is 200-300 mm; the impeller rotating speed of the microbubble generator is 1000-3000 rpm, preferably 2000-2500 rpm; the filler is one or more of a wire mesh, ceramic particles and a small Raschig ring.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111690520A (en) * | 2020-07-15 | 2020-09-22 | 北京诚益通控制工程科技股份有限公司 | Dynamic pipeline fermentation device and fermentation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111690520A (en) * | 2020-07-15 | 2020-09-22 | 北京诚益通控制工程科技股份有限公司 | Dynamic pipeline fermentation device and fermentation method |
| CN111690520B (en) * | 2020-07-15 | 2024-07-23 | 北京诚益通控制工程科技股份有限公司 | Dynamic pipeline fermentation device and fermentation method |
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