CN117339297A - Virus sample filters concentrator - Google Patents

Virus sample filters concentrator Download PDF

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Publication number
CN117339297A
CN117339297A CN202311504484.9A CN202311504484A CN117339297A CN 117339297 A CN117339297 A CN 117339297A CN 202311504484 A CN202311504484 A CN 202311504484A CN 117339297 A CN117339297 A CN 117339297A
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CN
China
Prior art keywords
filtering
primary
filtration
shell
air
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202311504484.9A
Other languages
Chinese (zh)
Inventor
谢金文
苗立中
唐娜
董林
毕研丽
王文秀
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Binzhou Animal Science & Veterinary Medicine Academy
Original Assignee
Shandong Binzhou Animal Science & Veterinary Medicine Academy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Binzhou Animal Science & Veterinary Medicine Academy filed Critical Shandong Binzhou Animal Science & Veterinary Medicine Academy
Priority to CN202311504484.9A priority Critical patent/CN117339297A/en
Publication of CN117339297A publication Critical patent/CN117339297A/en
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
    • B01D35/30—Filter housing constructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/50—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition
    • B01D29/56—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with multiple filtering elements, characterised by their mutual disposition in series connection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

The invention discloses virus sample filtering and concentrating equipment, which belongs to the technical field of filtering equipment and comprises a rotary disc, a primary filtering shell, a secondary filtering shell, a piston plate, an air cylinder and a liquid guide component.

Description

Virus sample filters concentrator
Technical Field
The invention belongs to the technical field of filtering equipment, and particularly relates to virus sample filtering and concentrating equipment.
Background
The virus sample is filtered and concentrated, the transfected virus stock solution is usually destroyed by overspeed centrifugal force, the broken cell debris solution is collected, and the virus components with different particle sizes can be obtained according to different centrifugal speeds by adopting a micron-sized filter membrane for filtration and purification.
Because the filtration and concentration of the virus solution are carried out by adopting the filter membranes with different filtration grades for multiple times, operators need to repeatedly load and unload the centrifuge tube on the centrifuge to carry out the filtration of the solution, so that the heavy operation process is increased, and the pollution of the sample solution is easily caused.
Disclosure of Invention
Aiming at the defects existing in the prior art, the embodiment of the invention aims to provide virus sample filtering and concentrating equipment so as to solve the problems in the background art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the virus sample filtering and concentrating equipment comprises a frame component, wherein the frame component comprises a main machine seat, a rotary disc and fixed shafts, the rotary disc is movably assembled on the main machine seat, and a plurality of fixed shafts are circumferentially arranged on the rotary disc;
the filtration assembly comprises a primary filtration shell, a secondary filtration shell and a piston plate, wherein the primary filtration shell is fixedly assembled at the tail part of the secondary filtration shell, the piston plate is assembled in the primary filtration shell in a sealing sliding way, a plurality of secondary filtration shells are circumferentially arranged on a rotary disc, the tail ends of the secondary filtration shells are rotationally assembled on a fixed shaft, and the plurality of groups of primary filtration shells and the secondary filtration shells are sequentially communicated with each other, so that virus sample stock solution to be filtered and concentrated sequentially flows along the plurality of groups of primary filtration shells and the secondary filtration shells in a unidirectional way;
the air pumping assembly comprises a plurality of air cylinders which are fixedly arranged at one end of the primary filtering shell and are communicated with the primary filtering shell, and the air pumping assembly is used for pumping air into the primary filtering shell;
and the liquid guide assembly is movably assembled on one side of the rotary disc, and is used for inputting the virus sample stock solution to be filtered and concentrated into the filtering assembly on the adjacent side and recovering the concentrated solution after multistage filtration.
As a further scheme of the invention, the filter assembly further comprises a clamping groove, a clamping plate and a filter membrane, wherein the clamping groove is arranged at one end of the secondary filter shell, the clamping plate is slidably assembled on the clamping groove, the filter membrane is fixedly assembled on the clamping plate, and the filter membrane is movably clamped on the secondary filter shell through a clamping seat.
As a further scheme of the invention, a plurality of secondary filter shells are internally provided with filter membranes with different filter grades, and the filter grade of the filter membranes is continuously increased in the flowing direction of concentrated virus sample stock solution to be filtered.
As a further aspect of the present invention, the filter assembly further includes a return spring disposed between the piston plate and the primary filter housing for driving the reciprocating motion of the piston plate, and an air vent disposed on a wall of the primary filter housing to define that air between the primary filter housing and the piston plate can flow out of the cavity of the primary filter housing in a unidirectional manner.
As a further scheme of the invention, the pumping assembly further comprises a gas valve head, a gas inlet hole, a branch pipe and a piston counterweight rod, wherein the gas valve head is fixedly arranged on one side of the gas cylinder and is communicated with the inner cavity of the gas cylinder, the gas inlet hole is arranged on the gas valve head and is used for limiting gas to flow into the gas cylinder in a unidirectional way, the gas valve head is also connected with the branch pipe, the tail end of the branch pipe is inserted into the primary filtering shell and is communicated with the primary filtering shell, the piston counterweight rod is also elastically inserted into the gas cylinder in a sliding way, and the piston at the tail end of the piston counterweight rod is slidingly assembled in the gas cylinder and is used for pumping the gas in the gas cylinder into the primary filtering shell.
As a further scheme of the invention, a first liquid inlet valve is further arranged on one side of the primary filter shell, a second liquid inlet valve is further arranged on one side of the secondary filter shell, the first liquid inlet valve is used for limiting the unidirectional inflow of fluid into the primary filter shell, the second liquid inlet valve is used for limiting the unidirectional outflow of fluid out of the secondary filter shell, the first liquid inlet valve and the second liquid inlet valve are respectively arranged at two ends of the filter membrane, and the first liquid inlet valve and the second liquid inlet valve are communicated between two groups of primary filter shells and secondary filter shells which are in any adjacent relation in the circumferential direction.
As a further scheme of the invention, the liquid guide component comprises a bracket, a liquid return cylinder and a liquid outlet cylinder, wherein the bracket is movably assembled on one side of the rotary disc, the liquid return cylinder and the liquid outlet cylinder are movably arranged on the bracket, the liquid return cylinder is connected with a backflow conduit, the backflow conduit is communicated with the secondary filter shell on the adjacent side, the liquid outlet cylinder is connected with an outflow conduit, and the outflow conduit is communicated with the primary filter shell on the other side.
In summary, compared with the prior art, the embodiment of the invention has the following beneficial effects:
according to the invention, the plurality of primary filter shells and secondary filter shells are circumferentially assembled on the rotary disc, and the filtering membranes with different filtering grades are arranged between the plurality of primary filter shells and the secondary filter shells, so that virus stock solution can continuously flow along a pipeline in the centrifugal crushing process, and multistage filtration and concentrated solution extraction are realized.
Drawings
Fig. 1 is a schematic diagram of a virus sample filtration and concentration apparatus provided in one embodiment of the invention.
Fig. 2 is a schematic structural diagram of a virus sample filtering and concentrating apparatus according to an embodiment of the present invention.
Fig. 3 is a schematic structural view of a schematic symbol a in a virus sample filtration and concentration apparatus according to an embodiment of the present invention.
Fig. 4 is a schematic structural view of a graphic mark B in a virus sample filtering and concentrating apparatus according to an embodiment of the present invention.
Fig. 5 is a schematic top structure view of a virus sample filtration and concentration device according to an embodiment of the present invention.
Reference numerals: 1-frame component, 101-main frame, 102-rotary disk, 103-fixed axle, 2-filter component, 201-primary filter shell, 202-secondary filter shell, 203-clamping groove, 204-clamping plate, 205-filter membrane, 206-clamping seat, 207-piston plate, 208-reset spring, 209-air vent, 210-first liquid inlet valve, 211-second liquid inlet valve, 3-pumping component, 301-inflator, 302-air valve head, 303-air inlet hole, 304-branch pipe, 305-piston balance weight rod, 4-liquid guide component, 401-bracket, 402-liquid return cylinder, 403-liquid outlet cylinder, 404-reflux conduit and 405-outflow conduit.
Detailed Description
In order to more clearly illustrate the structural features and efficacy of the present invention, the present invention will be described in detail below with reference to the accompanying drawings and examples.
Referring to fig. 1-5, a virus sample filtering and concentrating device in an embodiment of the present invention includes a frame member 1, where the frame member 1 includes a main frame 101, a rotary disc 102 and a fixed shaft 103, the main frame 101 is movably equipped with the rotary disc 102, and a plurality of fixed shafts 103 are circumferentially arranged on the rotary disc 102; the filtering assembly 2 comprises a primary filtering shell 201, a secondary filtering shell 202 and a piston plate 207, wherein the primary filtering shell 201 is fixedly assembled at the tail part of the secondary filtering shell 202, the piston plate 207 is assembled in the primary filtering shell 201 in a sealing sliding manner, a plurality of secondary filtering shells 202 are circumferentially arranged on the rotary disc 102, the tail ends of the secondary filtering shells 202 are rotationally assembled on the fixed shaft 103, and the groups of primary filtering shells 201 and the secondary filtering shells 202 are sequentially communicated with each other, so that virus sample stock solution to be filtered and concentrated sequentially flows along the groups of primary filtering shells 201 and the secondary filtering shells 202 in a unidirectional manner; the air pumping assembly 3, the air pumping assembly 3 comprises a plurality of air cylinders 301, the air cylinders 301 are fixedly arranged at one end of the primary filter shell 201 and are communicated with the primary filter shell 201, and the air pumping assembly is used for pumping air into the primary filter shell 201; and the liquid guide assembly 4 is movably assembled on one side of the rotary disc 102 and is used for inputting the virus sample stock solution to be filtered and concentrated into the filter assembly 2 on the adjacent side and recovering the concentrated solution after multistage filtration.
In practical application, when the virus sample stock solution is processed by the filtration and concentration device, firstly, the sample stock solution to be processed is input into the liquid guide component 4, and the driving motor in the main machine base 101 is started, so that the motor drives the rotary disc 102 to directionally rotate in the rotating process, when the rotary disc 102 drives a plurality of secondary filter shells 202 on the rotary disc, the air cylinder 301 assembled on the primary filter shell 201 at the tail end of the secondary filter shell 202 can slide relative to the air cylinder 301 under the action of centrifugal force, so that external air is sucked into the air cylinder 301, and when the motor is in variable speed motion, if the motor is decelerated, the piston weight rod 305 can reset towards one side of the air cylinder 301 under the action of elastic force, and the air in the air cylinder 301 is pumped into the primary filter shell 201 by utilizing the piston at the tail end of the piston weight rod 305, when gas enters the inside of the primary filter housing 201, the piston plate 207 can be pushed to move towards the side close to the filtering membrane 205 against the elastic force, so that the cavity of the primary filter housing 201 is in a positive pressure state, thereby the virus stock solution on one side of the primary filter housing 201 is pressed towards the side of the filtering membrane 205, when the rotation speed is increased, components in the virus stock solution can be centrifugally crushed in the process of following the centrifugal rotation of the rotary disc 102, and the piston plate 207 slides reversely under the centrifugal force, thereby the cavity of the primary filter housing 201 is in a negative pressure state, thereby the virus stock solution is sucked into the primary filter housing 201 along the first liquid inlet valve 210, and is sucked into the secondary filter housing 202 after filtering, and is sucked into the primary filter housing 201 on the adjacent side along the second liquid inlet valve 211, so that the virus stock solution continuously flows between the primary filter housing 201 and the secondary filter housing 202, realizing multistage centrifugal separation and filtration.
In one case of this embodiment, the driving motor in the main housing 101 is a variable speed motor, and the pumping gas in the air cylinder 301 can be controlled by the speed-down rotation after the high-speed centrifugal separation, and the specific principle of the motor will not be described here.
In one case of the present embodiment, a plurality of the secondary filter housings 202 are installed with filtering membranes 205 of different filtering grades, and the filtering grade of the filtering membranes 205 is continuously increased in the flowing direction of the concentrated virus sample stock solution to be filtered.
Referring to fig. 4, in a preferred embodiment of the present invention, the filter assembly 2 further includes a clamping groove 203, a clamping plate 204, and a filtering membrane 205, wherein the clamping groove 203 is disposed at one end of the secondary filter housing 202, the clamping plate 204 is slidably mounted thereon, the filtering membrane 205 is fixedly mounted on the clamping plate 204, and the filtering membrane 205 is movably clamped on the secondary filter housing 202 through a clamping seat 206.
In practical application, the clamping plate 204 is slidably assembled on the clamping groove 203, and the filtering membrane 205 is disposed on the clamping plate 204, so that the filtering membrane 205 with different filtering grades can be replaced according to different filtering requirements, and the filtering membrane 205 is movably buckled at one end of the clamping groove 203 through the buckling seat 206, so that the assembly and disassembly can be conveniently performed.
Referring to fig. 4, in a preferred embodiment of the present embodiment, the filter assembly 2 further includes a return spring 208, and an air vent 209, wherein the return spring 208 is disposed between the piston plate 207 and the primary filter housing 201 for driving the reciprocating motion of the piston plate 207, and the air vent 209 is disposed on a cavity wall of the primary filter housing 201, so as to limit that air between the primary filter housing 201 and the cavity of the piston plate 207 can flow out of the cavity of the primary filter housing 201 in a unidirectional manner.
In practical application, the return spring 208 is disposed between the primary filter housing 201 and the piston plate 207, and when the centrifugal force applied to the piston plate 207 is large, the piston plate can slide towards the end of the primary filter housing 201 against the elastic force of the return spring 208, and simultaneously, the residual air between the primary filter housing 201 and the piston plate 207 is exhausted along the air guide hole 209.
In one case of the present embodiment, the air guiding hole 209 has an air guiding amount far smaller than the air intake amount at the side of the primary filter housing 201, and only plays a role of balancing air pressure, and has a negligible influence on the air intake process.
Referring to fig. 3, in a preferred embodiment of the present invention, the pumping assembly 3 further includes a valve head 302, an air inlet 303, a branch pipe 304 and a piston weight rod 305, the valve head 302 is fixedly disposed on one side of the air cylinder 301 and is in communication with the inner cavity of the air cylinder 301, the air inlet 303 is disposed on the valve head 302 for limiting the unidirectional flow of air into the air cylinder 301, the branch pipe 304 is further connected to the valve head 302, the end of the branch pipe 304 is inserted into the primary filter housing 201 and is in communication with the primary filter housing 201, the piston weight rod 305 is further elastically slidably inserted into the air cylinder 301, and the piston at the end of the piston weight rod 305 is slidably mounted in the air cylinder 301 for pumping the air in the air cylinder 301 into the primary filter housing 201.
In practical application, when the piston weight rod 305 drives the piston at the end of the piston weight rod to slide in the air cylinder 301, external air can be sucked from the air inlet 303 into the air cylinder 301 when the air cylinder 301 is in a negative pressure state, and when the air cylinder 301 is in a positive pressure state, air in the air cylinder 301 can enter the primary filter housing 201 along the branch pipe 304, and the air valve head 302 can simultaneously limit the air flow direction on the air valve head 302 and the branch pipe 304.
Referring to fig. 3, in a preferred embodiment of the present invention, a first liquid inlet valve 210 is further disposed at one side of the primary filtering housing 201, a second liquid inlet valve 211 is further disposed at one side of the secondary filtering housing 202, the first liquid inlet valve 210 is configured to define a unidirectional inflow of fluid into the primary filtering housing 201, the second liquid inlet valve 211 is configured to define a unidirectional outflow of fluid out of the secondary filtering housing 202, and the first liquid inlet valve 210 and the second liquid inlet valve 211 are respectively disposed at two ends of the filtering membrane 205, and the first liquid inlet valve 210 and the second liquid inlet valve 211 are in communication between two groups of primary filtering housing 201 and secondary filtering housing 202 which are arbitrarily adjacent to each other in a circumferential direction.
In practical application, the primary filter housing 201 and the secondary filter housing 202 that are adjacent to each other in the circumferential direction may be connected by a hose, so that after the virus stock solution in any one group of primary filter housings 201 is filtered by the filter membrane 205 in one group of secondary filter housings 202, the filtered solution may pass through the second liquid inlet valve 211 and enter the other group of primary filter housings 201 for secondary filtration, so that the virus stock solution continuously flows in the plurality of filter membranes 205 with different filtering capacities, thereby realizing multi-stage filtration, and the filtered concentrate continuously flows along the pipeline and finally flows to the liquid return tube 402.
Referring to fig. 5, in a preferred embodiment of the present invention, the liquid guiding assembly 4 includes a support 401, a liquid return tube 402 and a liquid outlet tube 403, wherein the support 401 is movably assembled on one side of the rotary disc 102, the liquid return tube 402 and the liquid outlet tube 403 are movably mounted on the support, a backflow conduit 404 is connected to the liquid return tube 402, the backflow conduit 404 is in communication with the secondary filter housing 202 on the adjacent side, an outflow conduit 405 is connected to the liquid outlet tube 403, and the outflow conduit 405 is in communication with the primary filter housing 201 on the other side.
In practical application, the liquid return cylinder 402 may be used to collect the virus stock solution filtered by the multi-layer filtering membrane 205, and the liquid outlet cylinder 403 is used to hold the unfiltered virus stock solution and continuously drain the virus stock solution into the adjacent primary filter housing 201 under negative pressure.
In one case of this embodiment, the liquid return cylinder 402 and the liquid outlet cylinder 403 are both constant pressure bottles, so that when the bottle mouth is in a negative pressure suction state or a positive pressure inflow state, the air hole on the bottle body always ensures the constant bottle body pressure.
In the above embodiment of the present invention, a virus sample filtering and concentrating apparatus is provided, and a plurality of primary filter shells 201 and secondary filter shells 202 are circumferentially assembled on a rotary disc 102, and filtering membranes 205 with different filtering grades are arranged between the plurality of primary filter shells 201 and secondary filter shells 202, so that virus stock solution can continuously flow along a pipeline in the process of centrifugal crushing, thereby realizing multi-stage filtration and concentrated solution extraction.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.

Claims (7)

1. A virus sample filtration and concentration apparatus, the apparatus comprising:
the device comprises a frame component, a rotating disc and a plurality of fixing shafts, wherein the frame component comprises a main machine seat, the rotating disc and the fixing shafts, the rotating disc is movably assembled on the main machine seat, and the plurality of fixing shafts are circumferentially arranged on the rotating disc;
the filtration assembly comprises a primary filtration shell, a secondary filtration shell and a piston plate, wherein the primary filtration shell is fixedly assembled at the tail part of the secondary filtration shell, the piston plate is assembled in the primary filtration shell in a sealing sliding way, a plurality of secondary filtration shells are circumferentially arranged on a rotary disc, the tail ends of the secondary filtration shells are rotationally assembled on a fixed shaft, and the plurality of groups of primary filtration shells and the secondary filtration shells are sequentially communicated with each other, so that virus sample stock solution to be filtered and concentrated sequentially flows along the plurality of groups of primary filtration shells and the secondary filtration shells in a unidirectional way;
the air pumping assembly comprises a plurality of air cylinders which are fixedly arranged at one end of the primary filtering shell and are communicated with the primary filtering shell, and the air pumping assembly is used for pumping air into the primary filtering shell;
and the liquid guide assembly is movably assembled on one side of the rotary disc, and is used for inputting the virus sample stock solution to be filtered and concentrated into the filtering assembly on the adjacent side and recovering the concentrated solution after multistage filtration.
2. The virus sample filtering and concentrating device according to claim 1, wherein the filtering assembly further comprises a clamping groove, a clamping plate and a filtering membrane, the clamping groove is arranged at one end of the secondary filtering shell, the clamping plate is slidably assembled on the clamping groove, the filtering membrane is fixedly assembled on the clamping plate, and the filtering membrane is movably clamped on the secondary filtering shell through a clamping seat.
3. The virus sample filtration and concentration device according to claim 2, wherein a plurality of the secondary filter housings are provided with filtration membranes with different filtration grades, and the filtration grade of the filtration membranes is continuously increased in the flowing direction of the concentrated virus sample stock solution to be filtered.
4. The apparatus of claim 1, wherein the filter assembly further comprises a return spring disposed between the piston plate and the primary filter housing for driving the reciprocating motion of the piston plate, and an air vent disposed on a wall of the primary filter housing for defining a unidirectional outflow of air between the primary filter housing and the piston plate chamber out of the primary filter housing chamber.
5. The virus sample filtering and concentrating device according to claim 1, wherein the pumping assembly further comprises a valve head, an air inlet hole, a branch pipe and a piston counterweight rod, the valve head is fixedly arranged on one side of the air cylinder and is communicated with the inner cavity of the air cylinder, the air inlet hole is arranged on the valve head and is used for limiting the unidirectional flow of air into the air cylinder, the valve head is further connected with the branch pipe, the tail end of the branch pipe is inserted into the primary filtering shell and is communicated with the primary filtering shell, the piston counterweight rod is elastically and slidably inserted into the air cylinder, and the piston at the tail end of the piston counterweight rod is slidably assembled in the air cylinder and is used for pumping the air in the air cylinder into the primary filtering shell.
6. The virus sample filtering and concentrating apparatus according to claim 1, wherein a first liquid inlet valve is further provided on one side of the primary filtering housing, a second liquid inlet valve is further provided on one side of the secondary filtering housing, the first liquid inlet valve is used for limiting unidirectional inflow of fluid into the primary filtering housing, the second liquid inlet valve is used for limiting unidirectional outflow of fluid out of the secondary filtering housing, and the first liquid inlet valve and the second liquid inlet valve are respectively disposed at two ends of the filtering membrane, and between two groups of the primary filtering housing and the secondary filtering housing which are arbitrarily adjacent in a circumferential direction, and the first liquid inlet valve and the second liquid inlet valve on the first liquid inlet valve are communicated.
7. The virus sample filtering and concentrating apparatus of claim 1 wherein the liquid guide assembly comprises a bracket, a liquid return cylinder and a liquid outlet cylinder, wherein the bracket is movably assembled on one side of the rotary disc, the liquid return cylinder and the liquid outlet cylinder are movably arranged on the bracket, the liquid return cylinder is connected with a backflow conduit, the backflow conduit is communicated with the secondary filter shell on the adjacent side, the liquid outlet cylinder is connected with an outflow conduit, and the outflow conduit is communicated with the primary filter shell on the other side.
CN202311504484.9A 2023-11-13 2023-11-13 Virus sample filters concentrator Withdrawn CN117339297A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202311504484.9A CN117339297A (en) 2023-11-13 2023-11-13 Virus sample filters concentrator

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311504484.9A CN117339297A (en) 2023-11-13 2023-11-13 Virus sample filters concentrator

Publications (1)

Publication Number Publication Date
CN117339297A true CN117339297A (en) 2024-01-05

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Family Applications (1)

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CN202311504484.9A Withdrawn CN117339297A (en) 2023-11-13 2023-11-13 Virus sample filters concentrator

Country Status (1)

Country Link
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