CN117309781A - Endoscope smart mobile phone spectrometer based on optical fiber - Google Patents
Endoscope smart mobile phone spectrometer based on optical fiber Download PDFInfo
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- CN117309781A CN117309781A CN202210719681.1A CN202210719681A CN117309781A CN 117309781 A CN117309781 A CN 117309781A CN 202210719681 A CN202210719681 A CN 202210719681A CN 117309781 A CN117309781 A CN 117309781A
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- 239000013307 optical fiber Substances 0.000 title claims abstract description 50
- 239000000523 sample Substances 0.000 claims abstract description 39
- 238000005286 illumination Methods 0.000 claims abstract description 23
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000000835 fiber Substances 0.000 description 12
- 238000001228 spectrum Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 8
- 230000003287 optical effect Effects 0.000 description 7
- 241000220225 Malus Species 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 229930002877 anthocyanin Natural products 0.000 description 3
- 235000010208 anthocyanin Nutrition 0.000 description 3
- 239000004410 anthocyanin Substances 0.000 description 3
- 150000004636 anthocyanins Chemical class 0.000 description 3
- 235000021016 apples Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 235000021466 carotenoid Nutrition 0.000 description 3
- 150000001747 carotenoids Chemical class 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000000862 absorption spectrum Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229930002869 chlorophyll b Natural products 0.000 description 2
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229920000139 polyethylene terephthalate Polymers 0.000 description 2
- 239000005020 polyethylene terephthalate Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 239000000049 pigment Substances 0.000 description 1
- -1 polyethylene terephthalate Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000000985 reflectance spectrum Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mathematical Physics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Endoscopes (AREA)
Abstract
The invention discloses an endoscope smart phone spectrometer based on optical fibers, which comprises: the device comprises a probe shell, a smart phone and a handheld beam collector; the probe shell is fixedly connected with the handheld beam collector; a beam collecting optical fiber is arranged in the probe shell, and an illumination optical fiber is arranged between the beam collecting optical fiber and the probe shell; the front end of the light beam collecting optical fiber is provided with a front light beam collimating lens, and the rear end of the light beam collecting optical fiber is provided with a rear light beam collimating lens; the illumination optical fiber is connected with an LED light source of the smart phone through the light guide; a diffraction grating element, a cylindrical lens and a COMS camera are arranged in the handheld beam collector, and a fixed slit is correspondingly arranged between the diffraction grating element and the rear beam collimating lens; the cylindrical lens is orthogonal to the plane of the diffraction grating element; the cylindrical lens is arranged in a mode of being matched with the COMS camera, and the COMS camera is connected with the smart phone in a data mode. The device does not require additional provision of a battery while avoiding unnecessary stray illumination.
Description
Technical Field
The invention relates to the technical field of spectrum detection, in particular to an endoscope smart phone spectrometer based on optical fibers.
Background
The spectrum detection technology is a novel photoelectric detection technology, and a spectrometer based on the technology can perform quantitative and qualitative analysis on an object to be detected, so that the object can be detected rapidly, safely and nondestructively; the existing spectrometer on the market has huge volume and high price, and needs multiple people to operate, so that accurate measurement in a specific narrow space detection environment cannot be realized; more and more researchers are devoted to research and development of portable spectrometers, and solutions are provided for spectrometers in detection environments in specific narrow spaces; the usual direct Hue Saturation Value (HSV) color format is insufficient to determine the brightness of the screen image unless the responses of the Red, green and Blue three color channels (RGB channels) are recalibrated to reconstruct the actual response of the detector output; however, such solutions do not eliminate the effect of different background illumination on detection, and such small portable spectrometers also require the provision of separate light sources, power supplies and computer components.
Disclosure of Invention
The invention aims to solve the technical problems that an endoscope intelligent mobile phone spectrometer based on optical fibers is provided to solve the technical problems that the spectrometer in the prior art cannot eliminate the influence of different background illuminations on detection and needs to be provided with independent light sources, power sources and computer components.
In order to achieve the above object, the present invention provides an optical fiber-based endoscope smart phone spectrometer, comprising: the device comprises a probe shell, a smart phone and a handheld beam collector; the probe shell is fixedly connected with the handheld beam collector; a beam collection optical fiber is arranged in the probe shell, and an illumination optical fiber is arranged between the beam collection optical fiber and the probe shell; the front end of the light beam collecting optical fiber is provided with a front light beam collimating lens, and the rear end of the light beam collecting optical fiber is provided with a rear light beam collimating lens; the illumination optical fiber is connected with an LED light source of the smart phone through a light guide; a diffraction grating element, a cylindrical lens and a COMS camera are arranged in the handheld beam collector, and a fixed slit is correspondingly arranged between the diffraction grating element and the rear beam collimating lens; the cylindrical lens is orthogonal to the plane of the diffraction grating element; the cylindrical lens is arranged in a manner of being adaptive to the COMS camera, and the COMS camera is connected with the smart phone in a data mode.
Further, the illumination optical fibers are provided with six.
Further, the width of the fixing slit is 0.7mm.
Further, the density of the alternating pits of the diffraction grating element is G-722 lines/mm.
Further, the focal length of the cylindrical lens is f=2.0 cm.
Further, the COMS camera is connected with the smart phone through a Type-C transmission line.
Compared with the prior art, the invention has the following beneficial effects: according to the optical fiber-based endoscope smart phone spectrometer, the smart phone battery is used for supplying power to the whole system, and the endoscope probe can be directly abutted to a measured object so as to avoid unnecessary stray illumination; it is an additional advantage to be able to access hard-to-reach places such as the interior of transport cartons, the interior or back of crop bushes or the interior of the human torso. In addition, the function of the endoscope apparatus is expanded by the combined design of the slit and the optical element such as the lens.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a cross-sectional view at A in the present invention;
FIG. 3 is a schematic diagram of a calibration process for spectral data collected by a COMS camera;
FIG. 4 is a plot of normalized absorption intensity versus time for each individual RGB channel fitted;
reference numerals illustrate: the device comprises a 1-probe shell, a 2-smart phone, a 3-handheld beam collector, a 4-beam collecting optical fiber, a 5-illumination optical fiber, a 6-light guide, a 7-diffraction grating element, an 8-cylindrical lens, a 9-COMS camera, a 10-fixed slit and a 11-sample.
Detailed Description
The following describes the embodiments of the present invention further with reference to the drawings. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
Referring to fig. 1, a preferred embodiment of the present invention provides an optical fiber-based endoscopic smartphone spectrometer, comprising: a probe shell 1, a smart phone 2 and a handheld beam collector 3; the probe shell 1 is fixedly connected with the handheld beam collector 3; a beam collection optical fiber 4 is arranged in the probe shell 1, and six illumination optical fibers 5 are arranged between the beam collection optical fiber 4 and the probe shell 1; the front end of the light beam collecting optical fiber 4 is provided with a front light beam collimating lens (not shown in the figure), and the rear end of the light beam collecting optical fiber 4 is provided with a rear light beam collimating lens (not shown in the figure); the illumination optical fiber 5 is connected with an LED light source of the smart phone 2 through a light guide 6; a diffraction grating element 7, a cylindrical lens 8 and a COMS camera 9 are arranged in the handheld beam collector 3, and the density of alternate pits of the diffraction grating element 7 is G-722 lines/mm; the focal length of the cylindrical lens 8 is f=2.0 cm, a fixed slit 10 is correspondingly arranged between the diffraction grating element 7 and the rear beam collimating lens, and the width of the fixed slit 10 is 0.7mm; the cylindrical lens 8 is orthogonal to the plane of the diffraction grating element 7 and is used for improving the intensity of the signal collected by the COMS camera 9; the cylindrical lens 8 is arranged in a manner of being adapted to the COMS camera 9, and the COMS camera 9 is in data connection with the smart phone 2 through a Type-C transmission line. Light collection improvement of the detector by the cylindrical lens 8, and customized applications to handle the spectrum on the smartphone platform; the diffraction grating element 7 disperses the collimated light beam from the fixed slit 10 into a spectrum that is collected at the COMS camera 9; the fixed slit 10 is used to control the width of the collimated beam at the output end of the fiber, resulting in an optical resolution of δλ=2.0 nm; the illumination fiber 5 is provided only by the smartphone 2, transmitting to the sample to eliminate complications that may come from different background illuminations; the front beam collimating lens and the rear beam collimating lens are used to collimate a beam from an optically broadband source. The whole system is powered by the battery of the smart phone 2, so that an endoscope probe can be directly abutted to a measured object to avoid unnecessary stray illumination; it is an additional advantage to be able to access hard-to-reach places such as the interior of transport cartons, the interior or back of crop bushes or the interior of the human torso. Further, the function of the endoscope apparatus is expanded by the combined design of the fixing slit 10 and the optical element such as a lens.
The device uses the battery of the smart phone 2 to supply power for the whole system, thereby realizing the portable endoscope smart phone spectrometer. The proposed endoscope fiber optic bundle probe is flexible enough to move, probe and reach any surface; can be used to determine the optimal time for harvesting, transporting and storing agricultural products and ensure improved standardization and quality assessment; connecting an endoscope fiber optic fiber bundle probe and a handheld beam collector 3 assembly to a rear camera so that the rear camera is suitable for handheld operation; the used endoscope fiber bundle probe is directly connected with the measured object in a butt joint way, so that unnecessary stray illumination can be avoided; a cylindrical lens 8 orthogonal to the plane of the diffraction grating element 7 to collect and focus more light on the detector surface for improving the light collection per pixel on the detector in the diffraction direction; the actual response of the smart phone CMOS camera 9 is corrected by combining the customized mobile phone application program with a fitting equation, wherein the fitting equation is derived from a calibration factor obtained after the comparison with the single RGB channel response of the commercial portable spectrometer; the customized cell phone application takes into account the effect of all other optical elements on the excitation and emission light during the propagation of the light source to the detector, e.g., a polymeric light guide has weak absorption at shorter wavelengths of blue. Thus, the final spectral measurement is obtained by measuring the spectrum over the entire working span (λ -420-670 nm) with an Ocean Optics spectrometer and using the contrast differences for the correction factors for each wavelength in the application.
Embodiment one:
the embodiment provides an optical fiber-based endoscope smart phone spectrometer, and referring to fig. 1. The body of the endoscope spectrometer consists of a probe shell 1 of an endoscope optical fiber bundle, a smart phone 2, a light guide 6 and a handheld light beam collector 3. The probe housing 1 of the endoscope optical fiber bundle comprises an LED main light source for detecting the sample 11 by the illumination optical fiber 5, and is coupled into the endoscope optical fiber bundle probe by a flexible novel Polymer (PET) light guide made of 'highly durable' polyethylene terephthalate, and the front end and the rear end of the endoscope optical fiber bundle probe 4 respectively comprise a light beam collimating lens for collecting reflected or emitted light from the sample 11. The 3D printed handheld beam collector 3 is connected to the optical fiber output end, the light beam passes through the fixed slit 10 and reaches the COMS camera 9 for detection after diffraction, and finally, the spectrum data is transmitted to a mobile phone application program for analysis and processing.
Embodiment two:
the present embodiments provide a beam acquisition and data processing module for an endoscope spectrometer. Within the probe housing 1 of the endoscope optical fiber bundle, six illumination fibers 5 transmit light from the smartphone light source to the sample. Reflected or emitted light from the sample 11 is collected by a beam collection fiber 4, the beam collection fiber 4 being located in the center of the probe housing 1, a front beam collimating lens at the end of the beam collection fiber 4 for increased light collection, and a rear beam collimating lens for collimating the collected light; the output end of the endoscope probe uses a fixed slit 10 of width ω=0.7 mm to control the collimated beam width, resulting in an optical resolution δλ=2.0 nm; the light beam passes through a diffraction grating element 7, the diffracted light passes through a cylindrical lens 8 orthogonal to the grating, and finally the diffracted light is imaged and calibrated on a CMOS camera 9; after the custom application analyzes and processes the image from the grating diffraction and calibrates, the calibration process is shown in FIG. 3. The spectra measured over the entire working span (λ -420-670 nm) by the smartphone spectrometer and the Ocean Optical spectrometer were compared, the difference being used to apply a correction factor for each wavelength in the application.
To further illustrate the beneficial effects that can be achieved with the device, the reflectance spectrum of apples under normal supermarket storage conditions was measured using the device, the method comprising the steps of:
(1) Prior to capturing the spectrum of the apple, the reflection of the planar mirror is used for reference, and the absorption spectrum is determined by comparing the reflection of the apple with the reference reflection.
(2) Measuring spectrum (temperature T-22 ℃ and humidity H-50 percent) every 24 hours in a week, and using a fluorescent lamp of a common ceiling lamp to illuminate, wherein the illumination intensity is 400 lux and the distance is 3 meters; the visible light absorption spectrum of apples (just to store in a day) collected from fruit stores was recorded.
(3) After the smart device wirelessly transmits data to the smart phone, intensity changes are observed in the RGB channels, whose corresponding centers are near the absorption bands of λ -630, 540 and 440nm, respectively. These bands are closely related to changes in chlorophyll b, anthocyanin and carotenoid content within apples.
(4) The normalized absorption intensity variation Δi is calculated from the area under each fitted individual band.
FIG. 4 shows that these pigments decrease [ dI/dt (carotenoids): 0.14.+ -. 0.01 with corresponding decrease in absorption intensity over time; dI/dt (anthocyanin) is-0.09+/-0.01; dI/dt (chlorophyll) is-0.05+/-0.01 ]. The rate of decay of carotenoids and anthocyanins is greater than the rate of decay of chlorophyll b content. All of this determines the color, appearance and quality of the fruit. The results indicate that these parameters vary during storage as well as during transport at various pressures, including temperature.
From the above description, it can be seen that the above embodiments of the present invention achieve the following technical effects: the whole system is powered by the battery of the smart phone 2, so that an endoscope probe can be directly abutted to a measured object to avoid unnecessary stray illumination; it is an additional advantage to be able to access hard-to-reach places such as the interior of transport cartons, the interior or back of crop bushes or the interior of the human torso. Further, the function of the endoscope apparatus is expanded by the combined design of the fixing slit 10 and the optical element such as a lens. The assembly is connected to a COMS camera 9, making it suitable for hand-held operation; in addition, the endoscope fiber optic bundle probe is flexible enough to move, detect and reach any surface; the probe of the fiber optic bundle allows light to be collected from difficult to access areas and eliminates the effects of ambient lighting variations, making it an ideal choice for field diagnostics.
The embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, and yet fall within the scope of the invention.
Claims (6)
1. An optical fiber-based endoscope smartphone spectrometer, comprising: the intelligent mobile phone comprises a probe shell (1), a smart phone (2) and a handheld beam collector (3); the probe shell (1) is fixedly connected with the handheld light beam collector (3); a light beam collecting optical fiber (4) is arranged in the probe shell (1), and an illumination optical fiber (5) is arranged between the light beam collecting optical fiber (4) and the probe shell (1); the front end of the light beam collecting optical fiber (4) is provided with a front light beam collimating lens, and the rear end of the light beam collecting optical fiber (4) is provided with a rear light beam collimating lens; the illumination optical fiber (5) is connected with an LED light source of the smart phone (2) through a light guide (6); a diffraction grating element (7), a cylindrical lens (8) and a COMS camera (9) are arranged in the handheld beam collector (3), and a fixed slit (10) is correspondingly arranged between the diffraction grating element (7) and the rear beam collimating lens; the cylindrical lens (8) is orthogonal to the plane of the diffraction grating element (7); the cylindrical lens (8) is arranged in a manner of being matched with the COMS camera (9), and the COMS camera (9) is in data connection with the smart phone (2).
2. The optical fiber-based endoscopic smartphone spectrometer of claim 1, wherein: six illumination optical fibers (5) are arranged.
3. The optical fiber-based endoscopic smartphone spectrometer of claim 1, wherein: the width of the fixing slit (10) is 0.7mm.
4. The optical fiber-based endoscopic smartphone spectrometer of claim 1, wherein: the density of the alternating pits of the diffraction grating element (7) is G-722 lines/mm.
5. The optical fiber-based endoscopic smartphone spectrometer of claim 1, wherein: the focal length of the cylindrical lens (8) is f=2.0 cm.
6. The optical fiber-based endoscopic smartphone spectrometer of claim 1, wherein: the COMS camera (9) is connected with the smart phone (3) through a Type-C transmission line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210719681.1A CN117309781A (en) | 2022-06-23 | 2022-06-23 | Endoscope smart mobile phone spectrometer based on optical fiber |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210719681.1A CN117309781A (en) | 2022-06-23 | 2022-06-23 | Endoscope smart mobile phone spectrometer based on optical fiber |
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| Publication Number | Publication Date |
|---|---|
| CN117309781A true CN117309781A (en) | 2023-12-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210719681.1A Pending CN117309781A (en) | 2022-06-23 | 2022-06-23 | Endoscope smart mobile phone spectrometer based on optical fiber |
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| Country | Link |
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- 2022-06-23 CN CN202210719681.1A patent/CN117309781A/en active Pending
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