CN117304519A - Preparation method of silk fibroin microsphere and silk fibroin microsphere - Google Patents
Preparation method of silk fibroin microsphere and silk fibroin microsphere Download PDFInfo
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- CN117304519A CN117304519A CN202311203798.5A CN202311203798A CN117304519A CN 117304519 A CN117304519 A CN 117304519A CN 202311203798 A CN202311203798 A CN 202311203798A CN 117304519 A CN117304519 A CN 117304519A
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- 108010022355 Fibroins Proteins 0.000 title claims abstract description 124
- 239000004005 microsphere Substances 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 238000003756 stirring Methods 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 23
- 239000002245 particle Substances 0.000 claims abstract description 21
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims abstract description 19
- 229940057995 liquid paraffin Drugs 0.000 claims abstract description 19
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 19
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000004140 cleaning Methods 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 abstract description 46
- 238000011049 filling Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000010382 chemical cross-linking Methods 0.000 abstract description 3
- 239000003431 cross linking reagent Substances 0.000 abstract description 3
- 238000009776 industrial production Methods 0.000 abstract description 3
- 238000002347 injection Methods 0.000 abstract description 3
- 239000007924 injection Substances 0.000 abstract description 3
- 238000000502 dialysis Methods 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 5
- 239000008367 deionised water Substances 0.000 description 5
- 229910021641 deionized water Inorganic materials 0.000 description 5
- 238000000635 electron micrograph Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000001694 spray drying Methods 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/12—Powdering or granulating
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/12—Powdering or granulating
- C08J3/14—Powdering or granulating by precipitation from solutions
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a preparation method of silk fibroin microspheres and silk fibroin microspheres, wherein the preparation method of the silk fibroin microspheres comprises the following steps: providing a silk fibroin solution; uniformly mixing liquid paraffin, span 80 and tween 80 at the temperature of 35-60 ℃ to obtain a mixed oil phase; and (3) dropwise adding the silk fibroin solution into the mixed oil phase in a stirring state by using a syringe, continuously stirring for 30-90 min after the dropwise adding is finished, and separating to obtain the silk fibroin microspheres with the required particle size of 20-80 mu m. By combining the specific embodiment, the preparation method of the silk fibroin microsphere can realize stable batch preparation of micron-sized silk fibroin microspheres with the particle size of 20-80 mu m, and can be used for preparing injection filling preparations. In addition, the preparation method of the silk fibroin microsphere has the advantages of simple process, lower requirements on equipment, higher production efficiency, higher possibility of industrial production and no need of chemical crosslinking agent.
Description
Technical Field
The invention relates to the field of tissue repair materials, in particular to a preparation method of silk fibroin microspheres and the silk fibroin microspheres.
Background
Silk Fibroin (SF) is a natural protein polymer extracted from silk, and has been widely used in biomedical materials, cosmetics, pharmaceutical and other fields due to its excellent mechanical properties, biodegradability, biocompatibility. In addition, the silk fibroin can be processed into various material forms, such as films, microneedles, porous scaffolds, hydrogels, microspheres and the like, and can be widely applied to the fields of tissue engineering, wound dressing, drug-loaded slow release and the like.
Micron-sized microspheres with a particle size of 20-80 μm are currently commonly used as tissue repair materials or structural support materials, for example for the preparation of injection-filling formulations. When the particle size of the microsphere is too small, the microsphere is easily phagocytized by phagocytes, and when the particle size is too large, the syringe is easily blocked during compounding. With the recent intensive research on silk fibroin, it has become one of the natural materials widely used for preparing microspheres. At present, the preparation method of the silk fibroin microsphere mainly comprises an emulsification method, a spray drying method, a salting-out method, a phase separation method, an electric spraying method and the like, but how to prepare the micron-sized silk fibroin microsphere with the particle size of 20-80 μm is always a difficult point.
The Chinese patent CN 114874466A adopts the methods of balling agent balling, low-temperature solidification, wet-heat crystallization and spray drying to form the silk fibroin microsphere with the particle size of about 100 nm-5000 nm, and the method has complex process, and the prepared microsphere has smaller particle size, so that the application scene is limited. The silk fibroin porous microsphere with the diameter of 25-500 mu m is prepared by the Chinese patent No. CN 114306737A by using a microfluidic method and a freeze-drying method, and the method has higher requirements on equipment and lower production efficiency.
Disclosure of Invention
Based on this, it is necessary to provide a method for preparing silk fibroin microspheres that can solve the above-mentioned problems.
In addition, it is also necessary to provide a silk fibroin microsphere prepared by the preparation method of the silk fibroin microsphere.
A preparation method of silk fibroin microspheres comprises the following steps:
providing a silk fibroin solution, wherein the concentration of the silk fibroin solution is 5-20wt%;
uniformly mixing liquid paraffin, span 80 and tween 80 at the temperature of 35-60 ℃ to obtain a mixed oil phase, wherein the mass ratio of the liquid paraffin to the span 80 to the tween 80 is 100: 6-15: 2 to 5;
and (3) dropwise adding the silk fibroin solution into the mixed oil phase in a stirring state by using a syringe, continuously stirring for 30-90 min after the dropwise adding is finished, and separating to obtain the silk fibroin microsphere with the required particle size of 20-80 mu m, wherein the temperature of the mixed oil phase is 35-60 ℃.
In one embodiment, the mass ratio of the liquid paraffin, the span 80 and the tween 80 is 100:7.5 to 15:2.5 to 5.
In one embodiment, the concentration of the silk fibroin solution is 7.8wt% to 10wt%.
In one embodiment, the mass ratio of the liquid paraffin, the span 80 and the tween 80 is 100:7.5:2.5, the concentration of the silk fibroin solution is 7.8wt%.
In one embodiment, in the operation of dropwise adding the silk fibroin solution to the mixed oil phase in a stirred state by a syringe, the volume ratio of the silk fibroin solution to the mixed oil phase is 1:3 to 7.
In one embodiment, the silk fibroin solution is added dropwise to the mixed oil phase under stirring by a syringe, and the temperature of the silk fibroin solution is 45-50 ℃.
In one embodiment, the silk fibroin solution is dropwise added into the mixed oil phase in a stirring state by a syringe, and the stirring speed is 250 rpm-300 rpm in the operation of continuing stirring for 30 min-90 min after the completion of the dropwise addition.
In one embodiment, the silk fibroin solution is prepared by: sequentially degumming, dissolving, dialyzing and concentrating silk to obtain the silk fibroin solution.
In one embodiment, the operation of obtaining silk fibroin microspheres having a desired particle size of 20-80 μm after separation is: and sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microsphere with the required particle size of 20-80 mu m.
The silk fibroin microsphere is prepared by the preparation method of the silk fibroin microsphere.
By combining the specific embodiment, the preparation method of the silk fibroin microsphere can realize stable batch preparation of micron-sized silk fibroin microspheres with the particle size of 20-80 mu m, and can be used for preparing injection filling preparations.
In addition, the preparation method of the silk fibroin microsphere has the advantages of simple process, lower requirements on equipment, higher production efficiency, higher possibility of industrial production and no need of chemical crosslinking agent.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Wherein:
FIG. 1 is a flow chart of a method for preparing silk fibroin microspheres according to an embodiment.
FIG. 2 is an electron micrograph of silk fibroin microspheres prepared in example 1.
FIG. 3 is an electron micrograph of silk fibroin microspheres prepared in example 2.
FIG. 4 is an electron micrograph of silk fibroin microspheres prepared in example 3.
FIG. 5 is an electron micrograph of silk fibroin microspheres prepared in comparative example 1.
FIG. 6 is an electron micrograph of silk fibroin microspheres prepared in comparative example 2.
Detailed Description
The following description of the embodiments of the present invention will clearly and fully describe the technical solutions of the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present invention are merely used to explain the relative positional relationship between the members, the movement condition, etc. in a specific posture, and if the specific posture is changed, the directional indicators are correspondingly changed.
Furthermore, the description of "first," "second," etc. in this disclosure is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
Referring to FIG. 1, the invention discloses a preparation method of silk fibroin microspheres according to an embodiment, which comprises the following steps:
s10, providing a silk fibroin solution.
Wherein the concentration of the silk fibroin solution is 5-20wt%.
Too high or too low a concentration can affect the formation of silk fibroin microspheres.
Preferably, in this embodiment, the concentration of the silk fibroin solution is 7.8wt% to 10wt%.
More preferably, in this embodiment, the concentration of the silk fibroin solution is 7.8wt%.
Silk fibroin solutions are available directly from commercial sources.
In this embodiment, the silk fibroin solution is prepared by the following operations: sequentially degumming, dissolving, dialyzing and concentrating silk to obtain silk fibroin solution.
S20, uniformly mixing liquid paraffin, span 80 and tween 80 at the temperature of 35-60 ℃ to obtain a mixed oil phase.
Wherein, the mass ratio of liquid paraffin, span 80 and tween 80 is 100: 6-15: 2 to 5.
Too high or too low a concentration can affect the formation of silk fibroin microspheres.
Preferably, in the present embodiment, the mass ratio of liquid paraffin, span 80 and tween 80 is 100:7.5 to 15:2.5 to 5.
More preferably, in the present embodiment, the mass ratio of liquid paraffin, span 80 and tween 80 is 100:7.5:2.5.
s30, dropwise adding the silk fibroin solution into the mixed oil phase in a stirring state by using a syringe, continuously stirring for 30-90 min after the dropwise adding is finished, and separating to obtain the silk fibroin microspheres with the required particle size of 20-80 mu m.
The morphology of the droplets during the addition affects the formation of silk fibroin microspheres.
Wherein the temperature of the mixed oil phase is 35-60 ℃.
Preferably, in S30, the volume ratio of the silk fibroin solution to the mixed oil phase is 1:3 to 7.
More preferably, in S30, the volume ratio of the silk fibroin solution to the mixed oil phase is 1:5 to 6.
Preferably, in S30, the temperature of the silk fibroin solution is 45℃to 50 ℃.
Preferably, in S30, the stirring speed is 250rpm to 300rpm.
Too high or too low a rotational speed may result in too small or too large a particle size of the silk fibroin microspheres.
Specifically, the operation of obtaining silk fibroin microspheres with the required particle size of 20-80 μm after separation is as follows: and sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microsphere with the required particle size of 20-80 mu m.
By combining the specific embodiment, the preparation method of the silk fibroin microsphere can realize stable batch preparation of micron-sized silk fibroin microspheres with the particle size of 20-80 mu m, and can be used for preparing injection filling preparations.
In addition, the preparation method of the silk fibroin microsphere has the advantages of simple process, lower requirements on equipment, higher production efficiency, higher possibility of industrial production and no need of chemical crosslinking agent.
The invention also discloses an embodiment of the silk fibroin microsphere prepared by the preparation method of the silk fibroin microsphere.
The following are specific examples.
In a specific example, grade 5A silk was purchased from Rugao spring and autumn silk Inc., span 80, tween 80, liquid Paraffin were all purchased from Shanghai Ala Biochemical technologies Co.
Example 1
According to 1g:50mL of 5A-grade silk is immersed in Na with mass concentration of 0.06-0.2 wt% 2 CO 3 In the aqueous solution, the mixture is treated for 90 to 120 minutes at the temperature of between 98 and 100 ℃, taken out, cleaned and dried.
According to 1g: the degummed silk is dissolved in a ternary solvent according to the proportion of 15mL, and is poured into a dialysis bag with the molecular weight cut-off of 8kDa to 14kDa, deionized water is replaced every two hours, and dialysis is continued for 3 days, so that a silk fibroin solution is obtained, and concentration treatment is carried out, so that the silk fibroin solution with the concentration of 7.8wt% is obtained.
At 45 ℃, the mass ratio is 100:7.5: and 2.5, uniformly mixing the liquid paraffin, the span 80 and the tween 80 to obtain a mixed oil phase.
2mL of silk fibroin solution at 45 ℃ is dropwise added into 10mL of mixed oil phase under stirring by a syringe, and stirring is continued for 40min after the completion of the dropwise addition. Wherein the stirring speed was 300rpm.
And sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microspheres.
Example 2
According to 1g:50mL of 5A-grade silk is immersed in Na with mass concentration of 0.06-0.2 wt% 2 CO 3 In the aqueous solution, the mixture is treated for 90 to 120 minutes at the temperature of between 98 and 100 ℃, taken out, cleaned and dried.
According to 1g: the degummed silk is dissolved in a ternary solvent according to the proportion of 15mL, and is poured into a dialysis bag with the molecular weight cut-off of 8kDa to 14kDa, deionized water is replaced every two hours, and dialysis is continued for 3 days, so that a silk fibroin solution is obtained, and concentration treatment is carried out, so that the silk fibroin solution with the concentration of 10wt% is obtained.
At 45 ℃, the mass ratio is 100:12: and 4, uniformly mixing the liquid paraffin, the span 80 and the tween 80 to obtain a mixed oil phase.
1.8mL of silk fibroin solution at 48 ℃ is dropwise added into 10mL of mixed oil phase under stirring by a syringe, and stirring is continued for 40min after the dropwise addition is completed. Wherein the stirring speed was 250rpm.
And sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microspheres.
Example 3
According to 1g:50mL of 5A-grade silk is immersed in Na with mass concentration of 0.06-0.2 wt% 2 CO 3 In the aqueous solution, the mixture is treated for 90 to 120 minutes at the temperature of between 98 and 100 ℃, taken out, cleaned and dried.
According to 1g: the degummed silk is dissolved in a ternary solvent according to the proportion of 15mL, and is poured into a dialysis bag with the molecular weight cut-off of 8-14 kDa, deionized water is replaced every two hours, and dialysis is continued for 3 days, so that a silk fibroin solution is obtained, and concentration treatment is carried out, so that the silk fibroin solution with the concentration of 8wt% is obtained.
At 45 ℃, the mass ratio is 100:15: and 5, uniformly mixing the liquid paraffin, the span 80 and the tween 80 to obtain a mixed oil phase.
2mL of silk fibroin solution at 45 ℃ is dropwise added into 10mL of mixed oil phase under stirring by a syringe, and stirring is continued for 40min after the completion of the dropwise addition. Wherein the stirring speed was 300rpm.
And sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microspheres.
Comparative example 1
According to 1g:50mL of 5A-grade silk is immersed in Na with mass concentration of 0.06-0.2 wt% 2 CO 3 In the aqueous solution, the water-soluble polymer is mixed with the water,treating at 98-100 deg.c for 90-120 min, taking out, cleaning and drying.
According to 1g: the degummed silk is dissolved in a ternary solvent according to the proportion of 15mL, and is poured into a dialysis bag with the molecular weight cut-off of 8-14 kDa, deionized water is replaced every two hours, and dialysis is continued for 3 days, so that a silk fibroin solution is obtained, and concentration treatment is carried out, so that the silk fibroin solution with the concentration of 8wt% is obtained.
At 45 ℃, the mass ratio is 100:4.5:1.5 mixing liquid paraffin, span 80 and Tween 80 uniformly to obtain a mixed oil phase.
2mL of silk fibroin solution at 50 ℃ is dropwise added into 10mL of mixed oil phase under stirring by a syringe, and stirring is continued for 40min after the completion of the dropwise addition. Wherein the stirring speed was 250rpm.
And sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microspheres.
Comparative example 2
According to 1g:50mL of 5A-grade silk is immersed in Na with mass concentration of 0.06-0.2 wt% 2 CO 3 In the aqueous solution, the mixture is treated for 90 to 120 minutes at the temperature of between 98 and 100 ℃, taken out, cleaned and dried.
According to 1g: the degummed silk is dissolved in a ternary solvent according to the proportion of 15mL, and is poured into a dialysis bag with the molecular weight cut-off of 8-14 kDa, deionized water is replaced every two hours, and dialysis is continued for 3 days, so that a silk fibroin solution is obtained, and concentration treatment is carried out, so that the silk fibroin solution with the concentration of 8wt% is obtained.
At 45 ℃, the mass ratio is 100:18: and 6, uniformly mixing the liquid paraffin, the span 80 and the tween 80 to obtain a mixed oil phase.
1.8mL of silk fibroin solution at 45 ℃ is dropwise added into 10mL of mixed oil phase under stirring by a syringe, and stirring is continued for 40min after the dropwise addition is completed. Wherein the stirring speed was 300rpm.
And sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microspheres.
Test case
FIGS. 2 to 6 are scanning electron microscope images of silk fibroin microspheres prepared in examples 1 to 3 and comparative examples 1 to 2, respectively.
Referring to FIGS. 2 to 6, it can be seen that spherical silk fibroin microspheres can be prepared in examples 1 to 3, and the particle size is 20 μm to 80 μm, whereas comparative examples 1 to 2 cannot prepare complete silk fibroin microspheres.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the claims. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (10)
1. The preparation method of the silk fibroin microsphere is characterized by comprising the following steps:
providing a silk fibroin solution, wherein the concentration of the silk fibroin solution is 5-20wt%;
uniformly mixing liquid paraffin, span 80 and tween 80 at the temperature of 35-60 ℃ to obtain a mixed oil phase, wherein the mass ratio of the liquid paraffin to the span 80 to the tween 80 is 100: 6-15: 2 to 5;
and (3) dropwise adding the silk fibroin solution into the mixed oil phase in a stirring state by using a syringe, continuously stirring for 30-90 min after the dropwise adding is finished, and separating to obtain the silk fibroin microsphere with the required particle size of 20-80 mu m, wherein the temperature of the mixed oil phase is 35-60 ℃.
2. The method for preparing the silk fibroin microsphere according to claim 1, wherein the mass ratio of the liquid paraffin to the span 80 to the tween 80 is 100:7.5 to 15:2.5 to 5.
3. The method for preparing silk fibroin microspheres according to claim 2, wherein the concentration of the silk fibroin solution is 7.8wt% to 10wt%.
4. The method for preparing silk fibroin microspheres according to claim 3, wherein the mass ratio of the liquid paraffin to the span 80 to the tween 80 is 100:7.5:2.5, the concentration of the silk fibroin solution is 7.8wt%.
5. The method according to any one of claims 1 to 4, wherein the silk fibroin solution is added dropwise to the mixed oil phase in a stirred state by a syringe, the volume ratio of the silk fibroin solution to the mixed oil phase being 1:3 to 7.
6. The method according to claim 5, wherein the temperature of the silk fibroin solution is 45 ℃ to 50 ℃ in the operation of dropwise adding the silk fibroin solution into the mixed oil phase in a stirred state by a syringe.
7. The method according to claim 6, wherein the silk fibroin solution is added dropwise to the mixed oil phase in a stirred state by a syringe, and stirring is continued for 30 to 90 minutes after the completion of the addition, wherein the stirring speed is 200 to 400rpm.
8. The method for preparing silk fibroin microspheres according to claim 5, wherein the silk fibroin solution is prepared by: sequentially degumming, dissolving, dialyzing and concentrating silk to obtain the silk fibroin solution.
9. The method for preparing silk fibroin microsphere according to claim 5, wherein the operation of obtaining silk fibroin microsphere with a required particle size of 20-80 μm after separation is as follows: and sequentially cleaning the mixed system with ethanol and pure water, and freeze-drying to obtain the silk fibroin microsphere with the required particle size of 20-80 mu m.
10. A silk fibroin microsphere, characterized in that the silk fibroin microsphere is prepared by the preparation method of the silk fibroin microsphere according to any one of claims 1 to 9.
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| CN202311203798.5A CN117304519A (en) | 2023-09-15 | 2023-09-15 | Preparation method of silk fibroin microsphere and silk fibroin microsphere |
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