CN117303604A - Bacillus pedicel Meng Dayang and application thereof in aquaculture - Google Patents
Bacillus pedicel Meng Dayang and application thereof in aquaculture Download PDFInfo
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- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 39
- 238000009360 aquaculture Methods 0.000 title claims abstract description 33
- 244000144974 aquaculture Species 0.000 title claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000002360 preparation method Methods 0.000 claims abstract description 20
- 244000005700 microbiome Species 0.000 claims abstract description 13
- 241001465754 Metazoa Species 0.000 claims abstract description 9
- 230000004083 survival effect Effects 0.000 claims abstract description 9
- 241001331596 Oceanobacillus timonensis Species 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 238000009629 microbiological culture Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 9
- 230000000813 microbial effect Effects 0.000 claims description 7
- 241000428792 Caldimicrobium Species 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- 241000238553 Litopenaeus vannamei Species 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 108020004465 16S ribosomal RNA Proteins 0.000 description 5
- 241001467552 Mycobacterium bovis BCG Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229960000190 bacillus calmette–guérin vaccine Drugs 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 2
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 241000530454 Litopenaeus schmitti Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 240000002044 Rhizophora apiculata Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000005276 aerator Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000009642 citrate test Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009654 indole test Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Chemical & Material Sciences (AREA)
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- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Physiology (AREA)
- Hydrology & Water Resources (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention belongs to the field of microorganisms, and particularly relates to bacillus pedicel Meng Dayang YZS-M02 and application thereof in aquaculture. The Bacillus pedicel Meng Dayang YZS-M02 is classified and named as Meng Dayang Bacillus pedicel [ ]Oceanobacillus timonensis) YZS-M02 is preserved in China general microbiological culture Collection center (CGMCC) at 9 and 3 months in 2020, with preservation number of CGMCC No.20593 and preservation address of Beijing Chaoyang area North Chen Xiyu No.1 and 3. The aquaculture microorganism preparation prepared by utilizing the pedicel Meng Dayang bacillus YZS-M02 has the advantage of cleanThe capability of the aquaculture water body is improved, the aquaculture survival rate and the yield of the aquatic animals can be improved, and the aquaculture water body is beneficial to aquaculture and income.
Description
Technical Field
The invention belongs to the field of microorganisms, and relates to bacillus pedicel Meng Dayang and application thereof in aquaculture.
Background
In recent years, the aquatic culture industry rapidly develops and the intensification degree is continuously improved, and a large number of harmful algae and pathogenic bacteria in a culture pond are propagated in large quantity, so that the culture ecological environment is seriously damaged, and the culture diseases are frequently generated. The microecological preparation is a novel green and environment-friendly product, and has irreplaceable effects in regulating microecological balance of animal organism, preventing diseases, improving feed conversion rate, promoting animal health, protecting ecological environment, etc. At present, the microecological preparation is accepted as a green feed additive and is widely applied to aquaculture. The most widely used probiotics in the market are probiotics of yeasts, lactic acid bacteria and bacillus, have the effects of antagonizing intestinal pathogenic bacteria, maintaining and regulating intestinal microecological balance, can synthesize vitamins for hosts in the hosts, generate various digestive enzymes, provide nutrition and prevent invasion of pathogenic bacteria. The microecological preparation can promote animal growth, increase feed conversion rate and animal immunity. Therefore, the excavation and utilization of functional microorganisms are important components of efficient microecologics and important bases for functioning.
Disclosure of Invention
The invention aims to provide bacillus pedicel Meng Dayang and application thereof in aquaculture.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the application of the pedicel Meng Dayang bacillus YZS-M02 in preparing an aquaculture microbial preparation, wherein the pedicel Meng Dayang bacillus YZS-M02 is classified and named as pedicel Meng Dayang bacillus (Oceanobacillus timonensis) YZS-M02, and the pedicel Meng Dayang bacillus YZS-M02 is preserved in China general microbiological culture Collection center (CGMCC) with a preservation number of CGMCC No.20593 and a preservation address of North-West-Lu No.1 and 3 in the Korean of Beijing in the 9 month 3 day of 2020; the aquaculture microbial preparation has the effects of purifying aquaculture water and improving aquaculture survival rate and yield of aquatic animals.
An aquaculture microorganism preparation comprising bacillus caldus Meng Dayang YZS-M02, said aquaculture microorganism preparation being a liquid preparation,the living bacteria content range of the pedicel Meng Dayang bacillus YZS-M02 in the aquaculture microorganism preparation is (2-5) multiplied by 10 8 cfu/mL。
The application of the aquaculture microorganism preparation containing the bacillus caldus Meng Dayang YZS-M02 in purifying aquaculture water and improving aquaculture survival rate and yield of aquatic animals.
The invention has the remarkable advantages that:
the pedicel Meng Dayang bacillus YZS-M02 can effectively improve the transparency of the culture water body, and the water body purifying effect is obvious; can improve the survival rate and the yield of the cultured aquatic products.
Detailed Description
In order to make the contents of the present invention more easily understood, the technical scheme of the present invention will be further described with reference to the specific embodiments, but the present invention is not limited thereto.
In the following examples, unless otherwise specified, all experimental methods used are conventional and all materials, reagents, etc. are commercially available from biological or chemical reagent companies.
The formulation of the required media in the examples below is as follows:
NA medium: 10g of peptone, 3g of beef extract, 5g of NaCl, 15g of agar and 1000mL of water; the pH is 7.0-7.2.
NB medium: 10g of peptone, 3g of beef extract, 5g of NaCl and 1000mL of water; the pH is 7.0-7.2.
Example 1: separation, screening and identification of bacillus pedicel Meng Dayang
(1) And (3) separating and screening:
319 isolates were isolated from the Fujian mangrove plants by gradient dilution. Purifying the separated endophytic bacteria by a three-zone streak method, judging whether the bacterial strain is purified by microscopic examination, numbering the purified bacteria, picking single bacterial colonies, and transferring the single bacterial colonies to an NA culture medium inclined plane for later use. And then, simultaneously measuring activities of protease, lipase, amylase and cellulase and measuring degradation activities of ammonia nitrogen, nitrite, sulfide and COD on the separated strain, and finally screening endophytic bacteria with stronger activity, wherein the endophytic bacteria are marked as YZS-M02.
(2) Colony characteristics and colony morphology:
after YZS-M02 is cultured on an NA culture medium flat plate for 24 hours, a colony is pale yellow, matt, round, free of fluidity, gram-positive, spore-bearing and capsule-free; the colony diameter is 3-5mm.
(3) Physiological and biochemical characteristics:
YZS-M02 contact enzyme reaction is negative, V.P measurement is negative, MR measurement is positive, glucose acidogenesis test is negative, citrate test is negative, nitrate reduction is positive, starch hydrolysis is negative, indole test is negative, malonic acid measurement is negative, and H is produced 2 S test negative.
(4) 16S rDNA sequence analysis
The 16S rDNA gene sequence nucleotide sequence table SEQ ID NO.1 of the strain YZS-M02. The results of the alignment of the measured 16S rDNA sequences with sequences in the GenBank database show that: YZS-M02 and Oceanobacillus timonensis are on the same branch, and the similarity of 16S rDNA sequence and Oceanobacillus timonensis (MT 192497.1) reaches 99.72 percent. The strain YZS-M02 was initially identified as Bacillus pedicel Meng Dayang (Oceanobacillus timonensis) in combination with colony morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis.
Example 2: application of pedicel Meng Dayang bacillus YZS-M02 in purifying Penaeus vannamei Boone culture water, after pedicel Meng Dayang bacillus (Oceanobacillus timonensis) YZS-M02 is activated on NA culture medium slope, picking a loop, inoculating into NB culture medium, shake culturing at 28deg.C and 180r/min for 72 hr, diluting culture solution with sterile water to obtain culture solution containing 1×10 8 cfu/mL of bacterial liquid is used for standby.
Selecting an industrial indoor winter shed mode penaeus vannamei boone culture pond, wherein penaeus vannamei boone in the culture pond is young penaeus vannamei boone about 1 month. And (3) a test pool: application of 1X 10 8 cfu/mL of a bacillus calmette guerin YZS-M02 bacterial solution; control pool: no microbial preparation or other similar products are applied. And (3) a test pool: 20mL each day for 2 days. The bacillus calyx Meng Dayang YZS-M02 bacterial liquid is uniformly sprayed in a test pool, and the aerator is started for 2 hours after the spraying is finished. Periodically observing waterThe color changes, and the water changing proportion is reduced according to the water quality condition. During the test, other biological, chemical, disinfectant products and the like which influence the result are not added.
The results show that the water transparency of the test pool for applying the pedicel Meng Dayang bacillus YZS-M02 bacterial liquid is gradually improved along with the time, the water change amount of the test pool is reduced by 27% on the 2 nd day of the application of the pedicel Meng Dayang bacillus YZS-M02 bacterial liquid, the total water change amount is reduced by 35% on the following 3 days, and the difference is obvious compared with the control pool; the water color of the test pool to which the bacillus pedicel Meng Dayang YZS-M02 bacterial liquid is applied is light, and the difference is obvious compared with the light brown color of the water color of the control pool.
TABLE 1 purification effect of Di Meng Dayang bacillus YZS-M02 on culture water of Penaeus vannamei Boone
Example 3: influence of Bacillus calmette-guerin YZS-M02 on survival rate and yield of Penaeus vannamei Boone
Activating Bacillus pedicel Meng Dayang (Oceanobacillus timonensis) YZS-M02 on NA culture medium slope, selecting one ring, inoculating into NB culture medium, shake culturing at 28deg.C and 180r/min for 72 hr, diluting the culture solution with sterile water to obtain a solution containing 1×10 8 cfu/mL of bacterial liquid is used for standby.
2 treatments are selected from a penaeus vannamei boone culture pond (high-level pond), and the treatments are respectively as follows: and (3) a test pond: application of 1X 10 8 cfu/mL of a bacillus calmette guerin YZS-M02 bacterial solution; control pond: no microbial preparation or other similar products are applied. The average seedling weight of the test pond and the control pond is 4.6 g/tail, the cultivation area is 0.5 mu/pond, and the cultivation days are 90 days. The pedicel Meng Dayang bacillus YZS-M02 bacterial liquid and the feed are mixed evenly according to a proportion and then fed. The test pond is in the early stage of cultivation (seedling placement-cultivation for 60 days): mixing the bacillus caldus Meng Dayang YZS-M02 bacterial liquid and the feed according to the proportion of 5% (volume-mass ratio); in the later stage of cultivation (cultivation for 60 days- -fishing): the pedicel Meng Dayang bacillus YZS-M02 bacterial liquid and the feed are mixed according to the proportion of 8% (volume mass ratio). Periodic determination of water transparencyIndex of degree, ammonia nitrogen and nitrite nitrogen. During the test, other biological, chemical, disinfectant products and the like which influence the result are not added.
The result shows that the transparency of the water body of the test pond by applying the pedicel Meng Dayang bacillus YZS-M02 bacteria liquid is obviously improved, and the concentration of ammonia nitrogen and nitrite nitrogen in the water body is obviously reduced; the survival rate of the white shrimp in south America after the bacillus YZS-M02 bacterial liquid is applied to the pedicel Meng Dayang is 92%, which is improved by 16.46% compared with a control shrimp pond; after the pedicel Meng Dayang bacillus YZS-M02 bacteria liquid is applied, the qualification rate of normal shrimp bodies in the test pond is 69%, which is improved by 23.21% compared with a control pond; the yield of the shrimp pond per mu is improved by 19 percent.
TABLE 2 influence of Ti Meng Dayang Bacillus YZS-M02 on the purification of Penaeus vannamei Boone culture Water
TABLE 3 influence of Bacillus calmette-guerin YZS-M02 on survival rate and yield of Penaeus vannamei Boone
The foregoing description is only of the preferred embodiments of the invention, and all changes and modifications that come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (6)
1. The application of the bacillus caldus Meng Dayang YZS-M02 in preparing an aquaculture microorganism preparation is characterized in that: the Bacillus pedicel Meng Dayang YZS-M02 is classified and named as Meng Dayang Bacillus pedicel [ ]Oceanobacillus timonensis) YZS-M02 is preserved in China general microbiological culture Collection center (CGMCC) at 9 and 3 months in 2020, with preservation number of CGMCC No.20593 and preservation address of Beijing Chaoyang area North Chen Xiyu No.1 and 3.
2. The use according to claim 1, characterized in that: the aquaculture microbial preparation has the effects of purifying aquaculture water and improving aquaculture survival rate and yield of aquatic animals.
3. An aquaculture microorganism preparation comprising bacillus caldus Meng Dayang YZS-M02.
4. An aquaculture microbial agent according to claim 3, characterized in that: the aquaculture microorganism preparation is a liquid preparation.
5. An aquaculture microbial agent according to claim 4, wherein: the living bacteria content range of the pedicel Meng Dayang bacillus YZS-M02 in the aquaculture microorganism preparation is 2 multiplied by 10 8 ~5×10 8 cfu/mL 。
6. Use of an aquaculture microorganism preparation according to claim 3 for purifying aquaculture water and increasing aquaculture survival and yield of aquatic animals.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311532351.2A CN117303604A (en) | 2023-11-17 | 2023-11-17 | Bacillus pedicel Meng Dayang and application thereof in aquaculture |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311532351.2A CN117303604A (en) | 2023-11-17 | 2023-11-17 | Bacillus pedicel Meng Dayang and application thereof in aquaculture |
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