CN117299245A - Modularized integrated pressure-designating quantitative fluid driving chip for instant detection - Google Patents

Modularized integrated pressure-designating quantitative fluid driving chip for instant detection Download PDF

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Publication number
CN117299245A
CN117299245A CN202311463228.XA CN202311463228A CN117299245A CN 117299245 A CN117299245 A CN 117299245A CN 202311463228 A CN202311463228 A CN 202311463228A CN 117299245 A CN117299245 A CN 117299245A
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China
Prior art keywords
layer
fluid
pressure
chip
pressing
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CN202311463228.XA
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Chinese (zh)
Inventor
杜志昌
陈灵
杨绍辉
林忠华
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Jimei University
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Jimei University
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Priority to CN202311463228.XA priority Critical patent/CN117299245A/en
Publication of CN117299245A publication Critical patent/CN117299245A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers

Abstract

The invention provides a modularized integrated finger pressure quantitative fluid driving chip for instant detection, which comprises a batch manufacturing method of modularized integrated micro valves on the chip, a reusable finger pressure driving module for quantitatively driving the volume of fluid and a design of a micro-fluidic chip for instant detection. The main material of the micro valve is silicon rubber, and an anti-sticking film is used as a valve body; the finger-pressure driving module comprises a pressing part, a supporting part, a spring and a screw with a pressing head; the instant detection micro-fluidic chip comprises a three-layer structure, wherein the first layer is used for observing detection results, the second layer is used for constructing a fluid channel, and the third layer is used for sampling and constructing a micropump with a micro valve. According to the invention, the micro valve and the finger pressure driving module are combined on the instant detection micro-fluidic chip, so that mass production of the low-cost micro valve is realized, quantitative driving of fluid is realized by pressing the finger pressure driving module on the chip, the flow control precision of the micro fluid is ensured, and the accuracy of the on-site detection result of the chip is further improved.

Description

Modularized integrated pressure-designating quantitative fluid driving chip for instant detection
Technical Field
The invention relates to the technical field of microfluidic chips, in particular to a modularized integrated pressure-designating quantitative fluid driving chip for instant detection.
Background
The micro-fluidic chip technology is a micro-chip manufactured by utilizing a micro-nano processing technology, and the micro-sample is processed and analyzed by controlling the fluid flow in a micrometer-scale runner. It has great potential in biomedical, chemical analysis, environmental monitoring and other fields and is an indispensable tool in laboratory analysis and biomedical research. The traditional microfluidic chip system is complex, inconvenient to carry, high in cost and slow in response, so that the requirement of on-site instant detection cannot be met, and at the moment, research on driving and controlling of microfluidics is very necessary. Therefore, the invention focuses on the chip integrated micropump technology and fluid driving control method of the microfluidic chip.
The chip integrated micropump technology provides rich methods and tools for research and application in the field of microfluidics, and the types of micropumps include electric-driven micropumps, electrochemical-driven micropumps and pressure-driven micropumps. Electrically driven micropumps can precisely control flow and pressure, but require more complex circuitry and control systems; the electrochemical driving micropump has a compact structure and high mobility, but has slower response speed, and can be limited by bubble generation and chemical reaction in some application scenes; the pressure-driven micropump has a simple structure, is easier to modularly integrate on a chip, and meets the driving condition of high-flux fluid.
In the finger pressure driven micro-fluidic chip, the flow control method of the pressure driven micro-pump comprises a direct pressing method, an indirect control method and a sample injection control method. The direct pressing method ensures the simple structure of the chip, but can cause larger driving volume error due to the characteristics of individual fingers (finger force and pressing position); when the indirect control method controls fluid driving by using methods such as pressure transmission by setting an air pressure layer, energy conversion by a piezoelectric element, finger pressure control of capillary force and the like, the complexity of a chip is increased under the condition of ensuring certain driving precision, and the type of driving fluid is limited to a certain extent; the sample injection control method has higher driving precision, but needs higher pressing sensitivity, and the chip involves complex processing technology.
Disclosure of Invention
In view of the above, the present invention aims to provide a modular integrated pressure-designating quantitative fluid driving chip for instant detection, which utilizes a micro valve integrated on the chip in a modular manner, so as to improve portability of the chip, reduce manufacturing difficulty and processing cost of the micro valve, and make fluid driving no longer depend on external equipment such as a heavy external pump/power supply to provide input; the finger pressure driving module capable of quantitatively driving the volume of the fluid by repeated use enables the driving fluid to be simple to operate, and ensures the flow control precision of the fluid in the chip. The micro valve is integrated on the chip to construct the micro pump by the chip integrated micro pump technology, the finger pressure driving module is used for driving fluid, and the designed micro-fluidic chip can realize simple, rapid and high-precision on-site instant detection by combining the two modules.
In order to achieve the above purpose, the invention adopts the following technical scheme: a modular integrated pressure-designating fluid driving chip for instant detection comprises a modular integrated micro valve processed in a standardized way at low cost and an adjustable pressure-designating driving module; the modularized integrated micro valve is embedded into the quantitative fluid driving chip with the specified pressure, and the quantitative driving of multiple fluids on the chip is realized by pressing the adjustable quantitative specified pressure driving module by fingers.
In a preferred embodiment, the modular integrated microvalve is low cost, standardized by:
step 1: pouring a first layer of silicon rubber raw material (1) on a first die (2) and vacuumizing to eliminate bubbles; wherein a large number of semicircular ring structures (16) are arrayed and distributed on the first die (2);
step 2: placing the cover plate (3) on the first die (2) and the first layer of silicone rubber raw material (1) so as to flatten the first layer of silicone rubber raw material (1) to the same height as the arrayed semicircular ring structures (16); under the heat curing condition, vacuumizing to cure the first layer of silicon rubber raw material (1);
step 3: peeling the cover plate (3), and horizontally placing the fixed die (4) on the solidified first layer of silicon rubber raw material; placing an anti-sticking film (5) into the circular holes hollowed out by the array of the fixed die (4) to cover the first layer of cured silicone rubber raw material and the semicircular ring structure (16), wherein the anti-sticking film (5) is used as a valve body part of the micro valve;
step 4: removing the fixed die (4), placing the second die (6) on the solidified first layer of silicone rubber raw material at the position shown in the step 4, pouring the second layer of silicone rubber raw material (101) to a certain height, and then placing the third die (7) into the poured second layer of silicone rubber raw material (101);
step 5: under the heat curing condition, vacuumizing to cure the second layer of silicon rubber raw material (101), and finally sequentially stripping the third die (7), the second die (6) and the first die (2) respectively;
step 6: cutting by a tool to obtain a single complete one-way valve (8).
In a preferred embodiment, the first mould (2) comprises a bottom layer and a semicircular ring structure (16) distributed on the bottom layer in an array; the fixed die (4) is provided with an array hollowed-out round hole matched with the anti-adhesive film (5); said second mould (6) is a cylinder, the inner diameter of which determines the external dimensions of the non-return valve (8); the third mould (7) comprises a top layer and cylinders distributed on the top layer in an array; in the step 3, when the fixed die (4) is horizontally placed on the solidified first layer of silicon rubber raw material, the edge horizontal projection of the hole in the fixed die (4) is positioned at the outer circumference of the outer edge horizontal projection of the semicircular ring structure (16) and has a space, so that the anti-sticking film (5) can be used as a check valve body to be embedded into the check valve (8); when the anti-sticking film (5) is put into the fixed die (4), the edge of the anti-sticking film (5) is coincident with the edge of the hole in the fixed die (4).
In a preferred embodiment, the semicircular ring structure (16), the release film (5) and the third die (7) are coaxial.
In a preferred embodiment, the bottom layer of the first die (2) and the fixed die (4) are circular and have equal diameters.
In a preferred embodiment, the adjustable quantitative finger pressure driving module comprises a pressing part (9), a spring (10), a supporting part (11) and a screw (12), wherein a threaded hole (17) and a spring mounting hole (18) are arranged in the middle of the pressing part (9) so as to be matched with the installation of the screw (12) and the spring (10), the supporting part (11) is provided with the spring mounting hole (18) corresponding to the pressing part (9) so as to be matched with the installation of the spring (10), the top end and the bottom of the screw (12) are respectively provided with a rotary valve (19) and a pressing head (20), wherein when finger force acts on the pressing part (9), the spring (10) is compressed, further, the finger is pressed to the bottom, namely, the spring (10) is limited by the supporting part (11) after reaching the maximum compression amount, so that the same pressing depth can be ensured by pressing the finger pressure driving module each time, and quantitative driving of fluid is realized; the screw (12) can change the finger pressure stroke through rotating the rotary valve (19) to adjust the fluid volume driven by single pressing, so as to realize the adjustable function of the adjustable quantitative finger pressure driving module; the screw (12) can be provided with a plurality of pressing heads (20), and a plurality of fluids can be driven simultaneously by one pressing, so that one source and multiple drives are realized.
In a preferred embodiment, the threaded hole (17) is a through hole; the spring mounting holes (18) provided in the support portion (11) and the pressing portion (9) are the same in size; the maximum pressing depth position refers to a position when the screw (12) mounting upper surface coincides with the support portion (11) mounting lower surface; the pressing part (9), the supporting part (11) and the screw (12) are coaxial.
In a preferred embodiment, the fluid driving chip of the specified pressure and quantity comprises a three-layer structure: a bottom layer (13), a first layer of PDMS (14) and a second layer of PDMS (15); the bottom layer (13) is used for observing instant detection results, the first layer PDMS (14) is provided with a runner and a fluid treatment unit for instant detection, a plurality of one-way valve holes (24) are arranged in a key way, and the one-way valve holes (24) are embedded into a one-way valve standardized by a low-cost method of the modularized integrated micro valve through interference fit; unlike the first layer of PDMS (14), the second layer of PDMS (15) is further provided with a pressure driving chamber (25), wherein fluid flows in from a sample inlet (21) of the second layer of PDMS (15), a finger transmits force to the pressure driving chamber (25) of the second layer of PDMS (15) by pressing a pressing head (20) in the finger-pressing driving module, a pump membrane of the pressure driving chamber (25) vibrates to enable an embedded one-way valve (8) to be selectively opened or closed, the pressing part (9) is repeatedly pressed by the finger, a fluid sample enters a mixing runner (22) of the first layer of PDMS (14) through a micro valve to be fully mixed, and finally the mixed fluid flows to a detection hole (23) of the first layer of PDMS (14) to be detected in real time on site.
In a preferred embodiment, the sample injection hole (21), the detection hole (23) and the one-way valve hole (24) are through holes, so that the one-way valve (8) is embedded and sample injection and detection of sample reagents are realized; the pressure driving chamber (25) is spaced from the top of the second layer of PDMS (15); before the finger pressure driving module is pressed, the lower surface of the supporting part (11) is overlapped with the upper surface of the second layer PDMS (15), and the pressing head (20) is coaxial with the pressure driving chamber (25).
In a preferred embodiment, a single pressure-driven chamber (25) is in communication with both of the one-way valves (8) and may be configured as a micropump unit (26); two one-way valves (8) are placed in a positive-negative way and integrated on the first layer of PDMS (14) of the chip.
Compared with the prior art, the invention has the following beneficial effects: the invention provides a modularized integrated pressure-designating quantitative fluid driving chip for instant detection, which realizes the modularization and mass processing of micro valves on the chip and simultaneously can ensure the flow control precision of fluid in the chip. The micro valve consists of two parts, namely a material and a valve body, wherein the main material is silicon rubber, an anti-sticking film is used as the valve body, and the two micro valves are embedded into a chip in a positive-negative way, so that a micro pump unit can be simply constructed with a pressure driving cavity on the chip; the finger pressure driving module is used for quantitatively driving fluid, and the same pressing depth can be ensured when the finger pressure driving module is pressed to the limit position each time only by finger force, so that the volume of the fluid driven each time is ensured, and the flow control precision of the fluid is greatly improved; the design of the rotary valve at the top end of the screw can be used for adjusting the pressing depth, so that the quantitative adjustment of the volume of driving fluid is realized; through the combination of the two modules, the designed microfluidic chip can realize simple, rapid and high-precision on-site instant detection. The modularization and mass processing of the micro valve are realized, and the manufacturing difficulty and the processing cost of the micro valve are further reduced. The micro valve is miniaturized in size, and can be relatively simply integrated in a micro-fluidic chip to construct a micro-pump unit. The finger pressure driving module can quantitatively drive the volume of fluid, and has high flow control precision in the aspect of fluid driving; the volume of the driving fluid is quantitatively adjustable, the optimal pressing depth can be determined, and the field detection with higher efficiency is realized. The micro valve and the finger pressure driving module have good reusability and can be applied to other micro-fluidic chips.
Drawings
FIG. 1 is a flow chart of a method for mass production of on-chip modular integrated micro-valves in accordance with a preferred embodiment of the present invention;
FIG. 2 is an assembly view of a reusable, quantitatively driven fluid volume finger pressure drive module in accordance with a preferred embodiment of the present invention;
FIG. 3 is an assembly diagram of a real-time detection microfluidic chip according to a preferred embodiment of the present invention;
FIG. 4 is a schematic view showing the structure of the first mold in FIG. 1 according to a preferred embodiment of the present invention;
fig. 5 is a schematic view of the pressing part and the supporting part of fig. 2 according to a preferred embodiment of the present invention;
FIG. 6 is a schematic view of the screw of FIG. 2 in accordance with a preferred embodiment of the present invention;
FIG. 7 is a top view of the first and second PDMS layers of FIG. 3 according to a preferred embodiment of the present invention;
fig. 8 is a schematic diagram of the finger pressure driving module and the micro valve integrated on the chip and a cross-sectional view of the micro pump unit according to the preferred embodiment of the invention.
Detailed Description
The invention will be further described with reference to the accompanying drawings and examples.
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application; as used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
First, as shown in fig. 1 and fig. 4, the embodiment of the invention provides a method for manufacturing modularized integrated micro valves on a chip in batches, which comprises the following steps:
step 1: pouring a first layer of silicon rubber raw material 1 on a first die 2, and vacuumizing to eliminate bubbles; wherein, a large number of semicircular ring structures 16 are arrayed and distributed on the first die 2; wherein the first layer of silicone rubber material 1 may be, but is not limited to, PDMS material. The first mold 2 may be, but is not limited to, a glass mold.
Step 2: the cover plate 3 is placed over the first mould 2 and the first layer of silicone rubber stock 1 so that the first layer of silicone rubber stock 1 is flattened to the same height as the arrayed semicircular ring structure 16. Under the heat curing condition, the first layer of silicone rubber raw material 1 is cured by vacuumizing. Wherein the cover plate 3 may be, but is not limited to, a metal sheet. The heat curing conditions may be, but are not limited to, conditions at 90 ℃.
Step 3: the cover plate 3 is peeled off, and the fixed die 4 is laid flat on the cured first layer of silicone rubber raw material. The anti-sticking film 5 is placed into the circular holes hollowed out in the array of the fixed die 4, so that the anti-sticking film is covered on the solidified first layer of silicon rubber raw material and the semicircular ring structure 16, and the anti-sticking film 5 is used as a valve body part of the micro valve. Wherein the stationary mold 4 may be, but is not limited to, a glass mold. The release film 5 may be, but is not limited to, a silicon dioxide metal mask.
Step 4: the fixed mold 4 is removed, the second mold 6 is placed on the cured first layer of silicone rubber raw material at the position shown in step 4, the second layer of silicone rubber raw material (101) is poured to a certain height, and after that, the third mold 7 is placed in the poured second layer of silicone rubber raw material (101). Wherein the second layer of silicone rubber material (101) may be, but is not limited to, PDMS material. The second mold 6, the third mold 7 may be, but is not limited to, a glass mold.
Step 5: under the heat curing condition, the second layer of silicon rubber raw material (101) is cured by vacuumizing, and finally the third die 7, the second die 6 and the first die 2 are respectively peeled off in sequence.
Step 6: cutting by a tool to obtain a single complete one-way valve 8.
The first mold 2, the second mold 6, and the third mold 7 are not limited to being manufactured using a soft lithography process, and the molds may be manufactured by 3D printing using a metal material, for example. The first layer of silicon rubber raw material 1 and the second layer of silicon rubber raw material (101) can be replaced by other silicon rubber materials. The release film 5 may be replaced with other metallic or non-metallic materials and the process may be, but is not limited to, chemical vapor deposition.
As shown in fig. 1 and 4, in a preferred embodiment, the first mold 2 includes a bottom layer and a semicircular ring structure 16 arranged in an array on the bottom layer; the fixed die 4 is provided with an array hollowed-out round hole matched with the anti-adhesive film 5; said second mould 6 is a cylinder, the inner diameter of which determines the outer dimensions of the non-return valve; the third die 7 comprises a top layer and cylinders distributed on the top layer in an array; in step 3, when the fixed mold 4 is laid flat on the cured first layer of silicone rubber material, the horizontal projection of the edges of the holes in the fixed mold 4 is located outside the circumference of the horizontal projection of the outer edge of the semicircular ring structure 16 and has a spacing such that the anti-sticking film 5 can be embedded into the check valve 8 as a check valve body. When the release film 5 is placed in the fixed mold 4, the edge of the release film 5 should coincide with the edge of the hole in the fixed mold 4.
In a preferred embodiment, the semicircular ring structure 16, the release film 5 and the third mould 7 are coaxial.
In a preferred embodiment, the bottom layer of the first mold 2 and the fixed mold 4 are both circular and have equal diameters.
Next, as shown in fig. 2, 5 and 6, in a preferred embodiment, a reusable, adjustable compression stroke, finger pressure drive module for quantitatively driving a volume of fluid, comprises four parts: the device comprises a pressing part 9, a spring 10, a supporting part 11 and a screw 12, wherein a threaded hole 17 and a spring mounting hole 18 are formed in the middle of the pressing part 9 so as to be matched with the installation of the screw 12 and the spring 10, the spring mounting hole 18 corresponding to the pressing part 9 is formed in the supporting part 11 so as to be used for mounting the spring 10, a rotary valve 19 and a pressing head 20 are respectively arranged at the top end and the bottom of the screw 12, the spring 10 is compressed when a finger force acts on the pressing part 9, further, the finger is pressed to the bottom, namely, the spring 10 is limited through the supporting part 11 after reaching the maximum compression amount, so that the same pressing depth can be ensured when the finger pressing driving module is pressed each time, and the quantitative driving of fluid is realized.
In a preferred embodiment, the springs 10, 18 may be, but are not limited to, three; the pressing head 20 may be, but is not limited to, four; the rotary valve 19 may be, but is not limited to, an inline valve.
In a preferred embodiment, the pressing portion 9, the spring 10, the supporting portion 11, the screw 12 may be, but are not limited to, made of a metallic material.
In a preferred embodiment, the threaded hole 17 is a through hole; the spring mounting holes 18 provided in the support portion 11 and the pressing portion 9 are the same in size; the maximum pressing depth position refers to a position when the screw 12 mounting upper surface coincides with the support portion 11 mounting lower surface; the pressing portion 9, the supporting portion 11, and the screw 12 are coaxial.
Finally, as shown in fig. 3, 7 and 8, in a preferred embodiment, a finger-pressure driving microfluidic chip is characterized by comprising a three-layer structure: the bottom layer 13, the first layer PDMS14, and the second layer PDMS15, the bottom layer 13 is used for observing the instant detection result, the first layer PDMS14 is provided with a sample inlet 21, a mixing runner 22, a detection hole 23, and a one-way valve hole 24, so that a sample can be transported from the sample inlet to the detection hole as a fluid channel, unlike the first layer PDMS14, the second layer PDMS15 is further provided with a pressure driving chamber 25, wherein fluid flows from the sample inlet 21 of the second layer PDMS15, a finger applies a force to the pressure driving chamber 25 of the second layer PDMS15 through the pressing head 20 in the finger pressing driving module, the pressure driving chamber 25 pump film vibrates to enable the one-way valve 8 to be selectively opened/closed, the finger repeatedly presses the pressing part 9, the fluid sample enters the mixing runner 22 of the first layer PDMS14 through the micro valve to be fully mixed, and finally the mixed fluid flows to the detection hole 23 of the first layer PDMS14 to perform the instant detection on site.
In a preferred embodiment, the sample injection hole 21, the detection hole 23 and the one-way valve hole 24 are through holes, so that the one-way valve 8 is embedded and sample injection and detection of sample reagents are realized; the pressure driving chamber 25 is spaced from the top of the second layer PDMS 15; before the finger pressure driving module is pressed, the lower surface of the supporting part 11 is coincident with the upper surface of the second layer PDMS15, and the pressing head 20 is coaxial with the four pressure driving chambers 25; the sample inlet 21 and the pressure driving chamber 25 may be, but not limited to, four; the mixing flow channel 22 and the detection hole 23 may be, but are not limited to, two; the one-way valve bore 24 may be, but is not limited to being, eight.
In a preferred embodiment, a single pressure-driven chamber 25 is in communication with both of the one-way valves 8 to form a micropump unit 26.
In a preferred embodiment, the two check valves 8 are placed one on top of the other and integrated on the first layer of PDMS14 of the chip.
In a preferred embodiment, the immediate detection of blood type may be performed by integrating the micropump unit 26 and the finger pressure drive module on a chip. The method comprises the following steps: the blood sample, the anti-A reagent and the anti-B reagent are respectively injected through the injection holes 21, fingers repeatedly press the finger pressure driving module, quantitative fluid volume can be driven each time until the blood sample is respectively mixed with two antibodies in the mixing channel 22, and finally, the reaction mixture is respectively driven to the detection holes 23, and the blood type can be determined on site by observing the blood coagulation result; wherein the instant detection microfluidic chip can be used for instant detection of blood type but is not limited to.
It should be noted that relational terms such as first and second and the like are used solely to distinguish one entity or action from another entity or action without necessarily implying any actual such relationship or order between such entities or actions.
The background section of the present invention may contain background information about the problem or environment of the present invention, but is not necessarily descriptive of the prior art. Accordingly, inclusion in the background section is not an admission of prior art by the applicant.
The foregoing is a detailed description of the invention in connection with specific/preferred embodiments, and is not intended to limit the practice of the invention to such description. It will be apparent to those skilled in the art that several alternatives or modifications can be made to the described embodiments without departing from the spirit of the invention, and these alternatives or modifications should be considered to be within the scope of the invention.

Claims (10)

1. The modularized integrated pressure-designating quantitative fluid driving chip for instant detection is characterized by comprising a modularized integrated micro valve and an adjustable quantitative pressure-designating driving module, wherein the modularized integrated micro valve is processed in a standardized way at low cost; the modularized integrated micro valve is embedded into the quantitative fluid driving chip with the specified pressure, and the quantitative driving of multiple fluids on the chip is realized by pressing the adjustable quantitative specified pressure driving module by fingers.
2. A modular integrated, pressure-specific, fluid-driven chip for point-of-care testing as defined in claim 1, wherein said modular integrated microvalve is low cost, standardized in process by:
step 1: pouring a first layer of silicon rubber raw material (1) on a first die (2) and vacuumizing to eliminate bubbles; wherein a large number of semicircular ring structures (16) are arrayed and distributed on the first die (2);
step 2: placing the cover plate (3) on the first die (2) and the first layer of silicone rubber raw material (1) so as to flatten the first layer of silicone rubber raw material (1) to the same height as the arrayed semicircular ring structures (16); under the heat curing condition, vacuumizing to cure the first layer of silicon rubber raw material (1);
step 3: peeling the cover plate (3), and horizontally placing the fixed die (4) on the solidified first layer of silicon rubber raw material; placing an anti-sticking film (5) into the circular holes hollowed out by the array of the fixed die (4) to cover the first layer of cured silicone rubber raw material and the semicircular ring structure (16), wherein the anti-sticking film (5) is used as a valve body part of the micro valve;
step 4: removing the fixed die (4), placing the second die (6) on the solidified first layer of silicone rubber raw material at the position shown in the step 4, pouring the second layer of silicone rubber raw material (101) to a certain height, and then placing the third die (7) into the poured second layer of silicone rubber raw material (101);
step 5: under the heat curing condition, vacuumizing to cure the second layer of silicon rubber raw material (101), and finally sequentially stripping the third die (7), the second die (6) and the first die (2) respectively;
step 6: cutting by a tool to obtain a single complete one-way valve (8).
3. A modular integrated, pressure-specific, fluid-driven chip for on-line detection according to claim 2, characterized in that the first die (2) comprises a bottom layer and a semicircular ring structure (16) arranged in an array on the bottom layer; the fixed die (4) is provided with an array hollowed-out round hole matched with the anti-adhesive film (5); said second mould (6) is a cylinder, the inner diameter of which determines the external dimensions of the non-return valve (8); the third mould (7) comprises a top layer and cylinders distributed on the top layer in an array; in the step 3, when the fixed die (4) is horizontally placed on the solidified first layer of silicon rubber raw material, the edge horizontal projection of the hole in the fixed die (4) is positioned at the outer circumference of the outer edge horizontal projection of the semicircular ring structure (16) and has a space, so that the anti-sticking film (5) can be used as a check valve body to be embedded into the check valve (8); when the anti-sticking film (5) is put into the fixed die (4), the edge of the anti-sticking film (5) is coincident with the edge of the hole in the fixed die (4).
4. A modular integrated, pressure-specific, fluid-driven chip for on-line detection according to claim 2, characterized in that the semicircular ring structure (16), the release film (5), the third die (7) are coaxial.
5. A modular integrated, designated pressure fluid-driven chip for on-line detection according to claim 2, characterized in that the bottom layer of the first die (2) and the fixed die (4) are both circular and of equal diameter.
6. A modular integrated pressure-designating fluid driving chip for instant detection according to claim 1, characterized in that the adjustable quantity pressure-designating driving module comprises a pressing part (9), a spring (10), a supporting part (11) and a screw (12), wherein a threaded hole (17) and a spring mounting hole (18) are arranged in the middle of the pressing part (9) so as to be matched with the mounting of the screw (12) and the spring (10), the supporting part (11) is provided with a spring mounting hole (18) corresponding to the pressing part (9) so as to be matched with the mounting of the spring (10), the top end and the bottom of the screw (12) are respectively provided with a rotary valve (19) and a pressing head (20), wherein when finger force acts on the pressing part (9), the spring (10) is compressed, further, the finger is pressed to the bottom, namely, the spring (10) is limited through the supporting part (11) after reaching the maximum compression quantity, so that the same pressing depth can be ensured for realizing the quantitative driving of fluid;
the screw (12) can change the finger pressure stroke through rotating the rotary valve (19) to adjust the fluid volume driven by single pressing, so as to realize the adjustable function of the adjustable quantitative finger pressure driving module; the screw (12) can be provided with a plurality of pressing heads (20), and a plurality of fluids can be driven simultaneously by one pressing, so that one source and multiple drives are realized.
7. A modular integrated, pressure-specific, fluid-driven chip for on-demand detection according to claim 6, characterized in that the threaded hole (17) is a through hole; the spring mounting holes (18) provided in the support portion (11) and the pressing portion (9) are the same in size; the maximum pressing depth position refers to a position when the screw (12) mounting upper surface coincides with the support portion (11) mounting lower surface; the pressing part (9), the supporting part (11) and the screw (12) are coaxial.
8. A modular integrated fluid driver chip for point-of-care testing as recited in claim 1, wherein the fluid driver chip comprises a three-layer structure: a bottom layer (13), a first layer of PDMS (14) and a second layer of PDMS (15); the bottom layer (13) is used for observing instant detection results, the first layer PDMS (14) is provided with a runner and a fluid treatment unit for instant detection, a plurality of one-way valve holes (24) are arranged in a key way, and the one-way valve holes (24) are embedded into a one-way valve standardized by a low-cost method of the modularized integrated micro valve through interference fit; unlike the first layer of PDMS (14), the second layer of PDMS (15) is further provided with a pressure driving chamber (25), wherein fluid flows in from a sample inlet (21) of the second layer of PDMS (15), a finger transmits force to the pressure driving chamber (25) of the second layer of PDMS (15) by pressing a pressing head (20) in the finger-pressing driving module, a pump membrane of the pressure driving chamber (25) vibrates to enable an embedded one-way valve (8) to be selectively opened or closed, the pressing part (9) is repeatedly pressed by the finger, a fluid sample enters a mixing runner (22) of the first layer of PDMS (14) through a micro valve to be fully mixed, and finally the mixed fluid flows to a detection hole (23) of the first layer of PDMS (14) to be detected in real time on site.
9. A modular integrated, pressure-specific, fluid-driven chip for on-line detection according to claim 8, characterized in that the sample inlet (21), the detection orifice (23), the one-way valve orifice (24) are all through-holes for the insertion of the one-way valve (8) and the sample introduction and detection of sample reagents; the pressure driving chamber (25) is spaced from the top of the second layer of PDMS (15); before the finger pressure driving module is pressed, the lower surface of the supporting part (11) is overlapped with the upper surface of the second layer PDMS (15), and the pressing head (20) is coaxial with the pressure driving chamber (25).
10. A modular integrated, designated pressure fluid-driven chip for on-line detection according to claim 8, characterized in that a single pressure-driven chamber (25) is in communication with two of said one-way valves (8) and can be constructed as one micropump unit (26); two one-way valves (8) are placed in a positive-negative way and integrated on the first layer of PDMS (14) of the chip.
CN202311463228.XA 2023-11-06 2023-11-06 Modularized integrated pressure-designating quantitative fluid driving chip for instant detection Pending CN117299245A (en)

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