CN117298697A - Separation and collection device, collection system and collection method - Google Patents

Abstract

The application discloses separation collection device, collecting system and collection method, wherein, separation collection device includes: the body comprises a base, at least one pipe body and a cover body which are sequentially arranged, wherein the base is provided with a first cavity which is open towards one end of the pipe body, the pipe body is provided with a second cavity which is open at both ends, and the cover body is provided with a third cavity which is open towards one end of the pipe body; the filter assembly comprises a first filter membrane and a second filter membrane, the first filter membrane is arranged at the opening of the pipe body, which is close to the base, the second filter membrane is arranged at the opening of the pipe body, which is far away from the base, and the aperture of the second filter membrane is smaller than that of the first filter membrane; and the circulation driving assembly is used for driving the liquid in the first chamber, the second chamber and the third chamber to circulate outside the respective chambers. The method can realize multistage separation, purification and product collection of the product; meanwhile, the effects and the efficiency of multistage separation, purification and product collection can be improved.

Description

Separation and collection device, collection system and collection method

Technical Field

The application relates to the technical field of bioreactors, in particular to a separation and collection device, a collection system and a collection method.

Background

In the related art, a bioreactor is a device system for performing a biochemical reaction in vitro to obtain a metabolite by utilizing a biological function of an enzyme or an organism (such as a microorganism) by using a living cell or an enzyme as a biocatalyst to perform a cell proliferation or a biochemical reaction to provide a suitable environment. It is a biological function simulator, such as fermentation tank, immobilized enzyme or immobilized cell reactor, and is widely used in chemical industry, agriculture, food, medicine, etc.

The product of the bioreactor is a mixture, and the product is subjected to separation, purification and concentration to obtain a preparation stock solution, wherein the purification and concentration process mainly comprises the steps of affinity chromatography, low-PH incubation virus inactivation, multistage fine purification chromatography, virus removal nanofiltration, concentration ultrafiltration, sterile filtration and the like. However, the existing product separation and collection steps are complicated, and the collection effect is poor.

Disclosure of Invention

The present application aims to solve at least one of the technical problems existing in the prior art. Therefore, the application provides a separation and collection device, a collection system and a collection method, which can realize multistage separation, purification and product collection.

In one aspect, the present application provides a separation and collection device of an embodiment, including:

the body comprises a base, at least one pipe body and a cover body which are sequentially arranged, wherein the base is provided with a first cavity which is open towards one end of the pipe body, the pipe body is provided with a second cavity which is open at both ends, and the cover body is provided with a third cavity which is open towards one end of the pipe body; the base is provided with a first interface, the pipe body is provided with a second interface, and the cover body is provided with a third interface;

the filter assembly comprises a first filter membrane and a second filter membrane, the first filter membrane is arranged at the opening of the pipe body close to the base, the second filter membrane is arranged at the opening of the pipe body far away from the base, and the aperture of the second filter membrane is smaller than that of the first filter membrane;

and the circulating driving assembly is used for driving the liquid in the first chamber, the second chamber and the third chamber to circulate outside the respective chambers.

Further, the base is provided with a first inner support edge, one end of the pipe body, which is close to the base, is provided with a first connecting part which is connected with the first inner support edge in a matched mode, and the first filter membrane is arranged between the first inner support edge and the first connecting part.

Further, a second inner supporting edge is arranged at one end, far away from the base, of the tube body, and the second filter membrane is arranged on the second inner supporting edge; the cover body is provided with a second connecting part which is matched and connected with the second inner support edge near one end of the pipe body, and the second inner support edge is matched and connected with the second connecting part or the first connecting part of the other pipe body.

Further, the filter membrane filter further comprises a sealing assembly, wherein the sealing assembly comprises a first sealing ring, and the first sealing ring is arranged between the first filter membrane and the first inner supporting edge; and/or the first sealing ring is arranged between the second filter membrane and the second connecting part.

Further, the sealing assembly further comprises a second sealing ring, and the second sealing ring is arranged between the base and the pipe body; and/or the second sealing ring is arranged between the pipe body and the cover body; and/or, the second sealing ring is arranged between the pipe bodies.

Further, the base, the pipe body and the cover body are detachably connected.

Further, the first filter membrane and/or the second filter membrane is/are a titanium dioxide membrane, an alumina membrane, a silicon pore membrane or a polymer membrane.

Further, the device also comprises a vibrator, and the vibrator is connected with the base.

The utility model provides a collection system of embodiment is provided to another aspect of this application, including first peristaltic pump, coarse filtration device and separation collection device as previously described, the output of first peristaltic pump with coarse filtration device's input is connected, the base be provided with the first interface of first cavity intercommunication, coarse filtration device's output with first interface intercommunication.

In a further aspect, the present application provides an embodiment of a collecting method, applied to the foregoing collecting system, where the separation and collection device includes a vibrator, and includes the following steps:

sequentially passing the mixed stock solution through the first peristaltic pump, the coarse filtering device, the first chamber, the second chamber and the third chamber;

driving an external circulation of the liquid in the first chamber by using the circulation driving assembly;

driving the liquid in the second cavity to circulate outside by using the circulation driving assembly;

driving the liquid in the second cavity to circulate outside by using the circulation driving assembly;

respectively obtaining target filtrate in the first chamber, the second chamber and the third chamber;

in the filtering process, the vibrator is utilized to drive the base to vibrate, so that the liquid in the first chamber, the second chamber and the third chamber vibrate.

The separation and collection device, the collection system and the collection method have the following beneficial effects: when the products of the bioreactor are separated and collected, the concentrated product liquid is conveyed into a first cavity of the base from a first interface, and part of liquid in the first cavity can pass through a first filter membrane and enter a second cavity; part of the liquid in the second chamber passes through the second filter membrane into the third chamber. Because the pore size in the second filter membrane is smaller than the pore size in the first filter membrane, the filtrate with the particle size larger than the first filter membrane is separated in the first chamber, the filtrate with the particle size between the pore size in the first filter membrane and the pore size in the second filter membrane is separated in the second chamber, and the filtrate with the particle size smaller than the pore size in the second filter membrane can pass through the second filter membrane and enter the third chamber. Thus, the multistage separation, purification and product collection of the product can be realized. Meanwhile, the circulating driving assembly can drive filtrate in each cavity to circulate inside and outside the respective cavity, so that the filtrate is driven to flow, the filtrate with smaller particle size can conveniently pass through the filter membrane to enter other cavities, and the effects and the efficiency of multistage separation, purification and product collection are improved.

Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.

Drawings

The application is further described with reference to the accompanying drawings and examples, in which:

FIG. 1 is a schematic diagram of an exploded structure of a portion of a structure in one embodiment of the present application;

FIG. 2 is a schematic view of the structure of FIG. 1 from another perspective;

FIG. 3 is a schematic cross-sectional view of A-A of FIG. 2;

FIG. 4 is a schematic view of a base in an embodiment of the present application;

FIG. 5 is a schematic view of the structure of FIG. 4 from another perspective;

FIG. 6 is a schematic cross-sectional view of B-B of FIG. 5;

FIG. 7 is a schematic view of a tube body according to an embodiment of the present disclosure;

FIG. 8 is a schematic cross-sectional view of the tube of FIG. 7;

FIG. 9 is a schematic view of a cover according to an embodiment of the present disclosure;

FIG. 10 is a schematic cross-sectional view of the cover of FIG. 9;

FIG. 11 is a schematic workflow diagram of a collection system according to another embodiment of the present application.

Reference numerals:

110. a base; 111. a first interface; 112. a first inner support edge; 113. a first chamber; 114. a first clamping groove; 120. a tube body; 121. a first connection portion; 122. a second inner support edge; 123. a second chamber; 124. a second interface; 125. a second clamping part; 126. a second clamping groove; 130. a cover body; 131. a second connecting portion; 132. a third chamber; 133. a third interface; 134. a third clamping part;

210. a first filter membrane; 220. a second filter membrane;

310. a first seal ring; 320. a second seal ring;

410. a multistage peristaltic pump; 420. a vibrator;

510. a first peristaltic pump; 520. a coarse filtration device.

Detailed Description

Embodiments of the present application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for the purpose of explaining the present application and are not to be construed as limiting the present application.

In the description of the present application, it should be understood that references to orientation descriptions, such as directions of up, down, front, back, left, right, etc., are based on the orientation or positional relationship shown in the drawings, are merely for convenience of describing the present application and simplifying the description, and do not indicate or imply that the apparatus or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.

In the description of the present application, the meaning of a number is one or more, the meaning of a number is two or more, and greater than, less than, exceeding, etc. are understood to exclude the present number, and the meaning of a number above, below, within, etc. are understood to include the present number. The description of the first and second is for the purpose of distinguishing between technical features only and should not be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present application, unless explicitly defined otherwise, terms such as arrangement, installation, connection, etc. should be construed broadly and the specific meaning of the terms in the present application can be reasonably determined by a person skilled in the art in combination with the specific contents of the technical solution.

In the description of the present application, a description with reference to the terms "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

An embodiment of an aspect of the present application discloses a separation and collection device, see fig. 1 to 3, the separation and collection device includes a body, a filter assembly and a circulation driving assembly.

Specifically, the body includes a base 110, at least one pipe body 120 and a cover 130 that are sequentially disposed, the base 110 has a first chamber 113 with an opening facing one end of the pipe body 120, the pipe body 120 has a second chamber 123 with both ends open, and the cover 130 has a third chamber 132 with an opening facing one end of the pipe body 120.

Wherein, the base 110 is provided with a first interface 111 communicating with the first chamber 113, the tube 120 is provided with a second interface 124 communicating with the second chamber 123, and the cover 130 is provided with a third interface 133 communicating with the third chamber 132. The first port 111, the second port 124, and the third port 133 are used for introducing or discharging filtrate, or for externally circulating filtrate in the corresponding chamber and the circulation driving assembly.

It should be noted that the two adjacent components are in sealing connection so as to prevent the filtrate from directly realizing the cross-chamber communication transfer without a filter membrane; the tube 120 may be one or more. For the case that the number of the tube bodies 120 is plural, two sides of each tube body 120 are provided with filter membranes with different apertures, wherein the arrangement of the filter membranes follows that the aperture of the filter membrane close to the base 110 is larger than that of the filter membrane far away from the base 110 of the tube body 120, thus being beneficial to realizing multistage separation, collection or filtration.

In this embodiment, the outer circumference of the base 110 is provided with first ports 111 at intervals, one of which is used for inputting the mixture to be filtered. Further, the number of first ports 111 is two, one for inputting the concentrate mixture to be filtered, and the other first port 111 is for connecting with one end of the circulation driving assembly, and the other end of the circulation driving assembly. The circulation driving assembly is located outside the first chamber 113, and the two first interfaces 111 are communicated with the circulation driving assembly, so that filtrate in the first chamber 113 can circulate between the first chamber 113 and the circulation driving assembly. Similarly, two second ports 124 are provided at intervals on the outer periphery of the tube 120 for communication with the circulation driving assembly to enable filtrate in the second chamber 123 to circulate between the second chamber 123 and the circulation driving assembly.

It should be appreciated that only one third port 133 may be provided on the cover 130 for outputting the filtrate in the third chamber 132 to the outside. Two or more third interfaces 133 may also be provided, two of which are adapted to connect with the circulation drive assembly to circulate filtrate in the third chamber 132 between the third chamber 132 and the circulation drive assembly.

The filter assembly comprises a first filter membrane 210 and a second filter membrane 220, wherein the first filter membrane 210 is arranged at an opening of the tube body 120 close to the base 110, the second filter membrane 220 is arranged at an opening of the tube body 120 far away from the base 110, and the aperture of the second filter membrane 220 is smaller than that of the first filter membrane 210. It is understood that the filtrate component having a particle size smaller than the pore size of the filter membrane may pass through the filter membrane into another chamber.

The circulation driving assembly is used for driving the liquid in the first chamber 113, the second chamber 123 and the third chamber 132 to circulate outside in the respective chambers.

Specifically, the circulation driving component is disposed outside the base 110, the pipe 120 or the cover 130, and the circulation driving component can be respectively communicated with the first chamber 113, the second chamber 123 and the third chamber 132, so that the filtrate in each chamber flows through the circulation driving component from the corresponding chamber and then flows back into the corresponding chamber. So, can make the filtrate in each cavity circulate between cavity and circulation drive assembly to improve the mobility of the filtrate in the cavity, thereby make things convenient for the less filtrate of particle diameter to enter into in another cavity, help improving separation, filtration and the efficiency of collecting.

In particular, in this embodiment, the circulation driving assembly includes a multi-stage peristaltic pump 410, and one stage of driving circuit of the multi-stage peristaltic pump 410 is connected to two first interfaces 111 in the base 110 through a pipe, so as to form a circulation circuit. Further, another stage drive circuit of the multi-stage peristaltic pump 410 communicates with the two second ports 124 of the tube 120 to form a circulation circuit. It should be noted that the circulation loop formed by the first chamber 113 and the multi-stage peristaltic pump 410 is not in communication with the circulation loop formed by the second chamber 123 and the multi-stage peristaltic pump 410 to prevent filtrate from flowing through the circulation loop into the other chamber.

When the products of the bioreactor are separated and collected, the product solution is conveyed into the first chamber 113 of the base 110, and part of the liquid in the first chamber 113 can pass through the first filter membrane 210 and enter the second chamber 123; further, a portion of the liquid within second chamber 123 passes through second filter 220 into third chamber 132.

Since the pore size in the second filter 220 is smaller than the pore size in the first filter 210, the filtrate component having a larger particle size than the first filter 210 is partitioned in the first chamber 113, the filtrate having a particle size between the pore size in the first filter 210 and the pore size in the second filter 220 is partitioned in the second chamber 123, and the filtrate having a particle size smaller than the pore size in the second filter 220 can pass through the second filter 220 to enter the third chamber 132. Thus, the multistage separation, purification and product collection of the product can be realized. Meanwhile, the circulation driving assembly can drive filtrate in each cavity to realize external circulation in each cavity, drives the filtrate to flow, can facilitate the filtrate with smaller particle size to pass through the filter membrane and enter other cavities, and is beneficial to improving the effects and efficiency of multistage separation, purification and product collection.

In some embodiments of the present application, referring to fig. 3 to 6, the base 110 is provided with a first inner support edge 112, one end of the tube 120 near the base 110 is provided with a first connection portion 121 cooperatively connected with the first inner support edge 112, and the first filter membrane 210 is disposed between the first inner support edge 112 and the first connection portion 121.

In this embodiment, referring to fig. 3 to 6, the first inner support edge 112 may support the first filter membrane 210, the first connecting portion 121 of the tube body 120 may be embedded into the base 110, and then the top end surface of the first connecting portion 121 may abut against the first filter membrane 210 and press the first filter membrane 210 against the first inner support edge 112. The connection between the tube body 120 and the base 110 may be accomplished while also functioning to fix the first filter 210.

Further, referring to fig. 3 to 6 and 8, the inner periphery of the base 110 corresponding to the first connecting portion 121 is provided with a first clamping groove 114, the first clamping groove 114 is L-shaped, the outer periphery of the first connecting portion 121 is provided with a second clamping portion 125, the second clamping portion 125 is inserted into the first clamping groove 114, and the second clamping portion 125 and the first clamping groove 114 are rotated relatively, so that the fixed connection between the tube 120 and the base 110 can be realized.

In the case of having a plurality of pipes 120, the second clamping portion 125 may be inserted into the second clamping groove 126 of another pipe 120 to achieve connection between the pipes 120.

In some embodiments of the present application, referring to fig. 7 to 10, a second inner support edge 122 is disposed at an end of the tube 120 away from the base 110, and the second filter membrane 220 is mounted on the second inner support edge 122. The cover 130 is provided with a second connecting portion 131 near one end of the tube 120 and connected to the second inner support edge 122, and the second inner support edge 122 is connected to the second connecting portion 131.

It should be noted that, in the case where there are a plurality of the pipe bodies 120, the second inner rim 122 is used for being cooperatively connected with the first connection portion 121 of another pipe body 120.

In particular, in this embodiment, referring to fig. 7 to 9, a second clamping groove 126 is disposed at one end of the tube body 120 connected to the second connecting portion 131 of the cover body 130, the second clamping groove 126 is disposed in an L-shape, and a third clamping portion 134 is disposed at a position of the second connecting portion 131 corresponding to the second clamping groove 126. During assembly, the third clamping portion 134 can be placed in the second clamping groove 126, and then the third clamping portion 134 and the second clamping groove 126 are rotated relatively, so that the cover 130 and the tube 120 can be fixedly connected.

In some embodiments of the present application, the separation and collection device further includes a sealing assembly, where the sealing assembly includes a first sealing ring 310, and the first sealing ring 310 is installed between the first filter membrane 210 and the first inner support edge 112 to improve tightness.

In some embodiments of the present application, a first sealing ring 310 is also disposed between the second filter membrane 220 and the second connecting portion 131 to improve the sealing performance.

In some embodiments of the present application, the seal assembly further includes a second seal ring 320, the second seal ring 320 being disposed between the base 110 and the tube 120.

As one embodiment, referring to fig. 3 and 4, the pipe body 120 has a first connection part 121, and the first connection part 121 is stepped with the outer circumference of the pipe body 120. During assembly, the second sealing ring 320 is sleeved on the outer periphery of the first connecting portion 121, and when the first connecting portion 121 is embedded into the base 110, the second sealing ring 320 abuts against the end face of the base 110, which is close to the pipe body 120.

In some embodiments of the present application, the second seal 320 is disposed between the tube 120 and the cap 130.

As one embodiment, referring to fig. 3 and 4, the cover 130 has a second connection portion 131, and the second connection portion 131 is stepped with the outer circumference of the cover 130. When assembled, the second sealing ring 320 is sleeved on the outer periphery of the second connecting part 131, and when the second connecting part 131 is embedded into the pipe body 120, the second sealing ring 320 is abutted with the end face of the pipe body 120, which is close to the cover body 130.

In some embodiments of the present application, the second sealing ring 320 may also be disposed between the pipe body 120 and the pipe body 120 to improve the connection tightness between the pipe body 120 and another pipe body 120.

It should be noted that in the foregoing embodiments, any two of the base 110, the tube 120, and the cover 130 are detachably connected. Specifically, the base 110 is detachably connected with the pipe body 120; the cover 130 is also detachably connected to the tube 120. In the case of multiple tubes 120, there is also a removable connection between two adjacent tubes 120.

It should be appreciated that the detachable connection between the components may be achieved by the above-mentioned manner of matching connection between the clamping groove and the clamping portion, or may be achieved by a threaded connection, a bolt connection, or the like, which is not limited herein.

In some embodiments of the present application, the first filter membrane 210 is a titania membrane or an alumina membrane, particularly a titania membrane prepared by CN202111582986.4 or an alumina membrane prepared by CN 202210868209.4. Further, the second filter 220 is a titanium dioxide membrane, an alumina membrane, a silicon pore membrane, or a polymer membrane including, but not limited to, polytetrafluoroethylene membrane or polysulfone membrane, polyethersulfone membrane, or the like. The pore sizes of the first filter 210 and the second filter 220 can be selected according to the actual separation, purification and product collection requirements. Illustratively, the pore size of the first filter 210 may be selected to be greater than or equal to 200nm, the pore size of the second filter 220 may be selected to be less than or equal to 30nm, and thus, the resulting 3 products are respectively filtrate (primary filtrate) containing biological samples with particle sizes of >200nm, and filtrate (primary filtrate) containing biological samples with particle sizes of 30-200 nm; a filtrate (secondary filtrate) containing biological samples with particle size <30 nm.

It is appreciated that the resulting products within each chamber may be exported through the first, second and third interfaces 111, 124 and 133, respectively.

In some embodiments of the present application, the separation and collection device further includes a vibrator 420, wherein the vibrator 420 is connected to the base 110. Specifically, the vibrator 420 vibrates the base 110 by driving the base 110 to vibrate, and the base 110 vibrates the tube 120 and the cover 130. In this way, the contaminants adsorbed on the surfaces of the first filter membrane 210, the second filter membrane 220 and the like can be stripped, so that the purpose of cleaning the surfaces of the membranes can be achieved, the filtering effect of the filter membranes can be ensured, and the service life of the filter membranes can be prolonged.

In this embodiment, the vibrator 420 is an ultrasonic transducer, which can convert the sound energy of the power ultrasonic source into mechanical vibration, and then radiate the sound wave to the filtrate in each chamber through the walls of the base 110, the tube 120 and the cover 130. Under the action of ultrasonic waves, the interior of the filtrate is compressed and generates micro bubbles, and the micro bubbles are compressed and crushed to generate ultrasonic cavitation effect. The micro-bubbles generated in the filtrate keep vibrating under the action of sound waves, so that the adsorption of pollutants on the surface of the filter membrane can be destroyed, and finally the pollutants are peeled off from the surface of the filter membrane, thereby achieving the purpose of cleaning the surface of the filter membrane.

Referring to fig. 11, the collecting system according to another embodiment of the present application includes a first peristaltic pump 510, a coarse filtering device 520 and a separating and collecting device as before, an output end of the first peristaltic pump 510 is connected to an input end of the coarse filtering device 520, a base 110 is provided with a first interface 111 communicating with the first chamber 113, and an output end of the coarse filtering device 520 is communicated with the first interface 111.

In one embodiment, the coarse filtration device 520 includes a concentrate filter that uses a pressure differential to drive the stock solution through a filter membrane, where the solute on the filter membrane is progressively enriched and eventually forms a concentrate.

In one embodiment, the separation and collection device includes a tube 120. The application principle is as follows:

separating mixed stock solution of biological samples (such as proteins, polypeptides, nucleic acids, saccharides, exosomes and the like) from samples such as cells or cell supernatant and the like, and primarily concentrating the mixed stock solution through a coarse filtering device 520 under the extrusion pushing action of a first peristaltic pump 510 to remove water and salt; the obtained concentrated solution enters a separation and collection device.

Specifically, the specific flow direction of the concentrated solution in the separation and collection device is as follows: the concentrated solution enters the first chamber 113 from the first interface 111 of the base 110, and then passes through the first filter membrane 210 (for example, the filter membrane aperture of the first filter membrane 210 is greater than or equal to 200 nm) to perform first-stage filtration, so that the generated first-stage filtrate is primary refined filtrate, and the concentrated solution remained in the base 110 is primary refined filtrate. The first-stage filtrate is then passed through a second filter 220 (e.g., the second filter 220 has a filter pore size of 30nm or less) for second-stage filtration, and the resulting second-stage filtrate is discharged from the system.

In the above examples, the 3 products finally obtained are respectively: a filtrate (primary filtrate) containing biological samples with particle diameters of >200nm, a filtrate (primary filtrate) containing biological samples with particle diameters of 30-200 nm; a filtrate (secondary filtrate) containing biological samples with particle size <30 nm.

Wherein, the primary fine filtrate is located in the first chamber 113, the primary filtrate is located in the second chamber 123, and the secondary filtrate is located in the third chamber 132. The resulting products in each chamber may be exported via the first port 111, the second port 124, and the third port 133, respectively, or may continue to circulate with the fluid until the desired concentration is collected and then cease to be exported.

In this embodiment, the collecting and separating device is detachable, so that the filter membranes with different apertures can be replaced and one or more of the tube bodies 120 can be stacked as required, so as to realize separation, filtration and product collection of products with different particle sizes.

For example, when it is necessary to further precisely intercept a substance in the range of 60 to 120nm, 2 tubes 120 may be connected. The pore diameters of the filter membranes at the two ends of the first pipe body 120 are 0.45 μm and 0.2 μm in sequence, the obtained filtrate enters the second pipe body 120, and the pore diameters of the filter membranes at the two ends of the second pipe body 120 are 120nm and 60nm in sequence. Thus, the filtrate in the second tube 120 is the target sample.

In another aspect, the present disclosure discloses a collecting method, which is applied to the collecting system, and the separating and collecting device includes a vibrator 420, including the following steps: sequentially passing the mixed stock solution through a first peristaltic pump 510, a coarse filtration device 520, a first chamber 113, a second chamber 123 and a third chamber 132; driving the liquid in the first chamber 113 to perform external circulation by using the circulation driving assembly; driving the liquid in the second chamber 123 to perform external circulation by using the circulation driving assembly; driving the liquid in the second chamber 123 to perform external circulation by using the circulation driving assembly; the target filtrate is obtained in the first chamber 113, the second chamber 123, and the third chamber 132, respectively.

In the filtering process, the vibrator 420 is utilized to drive the base 110 to vibrate, so that the liquid in the first chamber 113, the second chamber 123 and the third chamber 132 vibrate.

The embodiments of the present application have been described in detail above with reference to the accompanying drawings, but the present application is not limited to the above embodiments, and various changes can be made within the knowledge of one of ordinary skill in the art without departing from the spirit of the present application. Furthermore, embodiments of the present application and features of the embodiments may be combined with each other without conflict.

Claims (10)

1. A separation and collection device, comprising:

the body comprises a base, at least one pipe body and a cover body which are sequentially arranged, wherein the base is provided with a first cavity which is open towards one end of the pipe body, the pipe body is provided with a second cavity which is open at both ends, and the cover body is provided with a third cavity which is open towards one end of the pipe body; the base is provided with a first interface, the pipe body is provided with a second interface, and the cover body is provided with a third interface;

the filter assembly comprises a first filter membrane and a second filter membrane, the first filter membrane is arranged at the opening of the pipe body close to the base, the second filter membrane is arranged at the opening of the pipe body far away from the base, and the aperture of the second filter membrane is smaller than that of the first filter membrane;

and the circulation driving component is used for driving the liquid in at least one of the first chamber, the second chamber and the third chamber to circulate outside the respective chambers.

2. The separation and collection device according to claim 1, wherein the base is provided with a first inner support edge, one end of the tube body, which is close to the base, is provided with a first connecting portion, which is connected with the first inner support edge in a matched manner, and the first filter membrane is arranged between the first inner support edge and the first connecting portion.

3. The separation and collection device according to claim 2, wherein a second inner support edge is arranged at one end of the tube body away from the base, and the second filter membrane is mounted on the second inner support edge; the cover body is provided with a second connecting part which is matched and connected with the second inner support edge near one end of the pipe body, and the second inner support edge is matched and connected with the second connecting part or the first connecting part of the other pipe body.

4. The separation and collection device of claim 3, further comprising a seal assembly comprising a first seal ring mounted between the first filter membrane and the first inner rim; and/or the first sealing ring is arranged between the second filter membrane and the second connecting part.

5. The separation and collection device of claim 4, wherein the seal assembly further comprises a second seal ring disposed between the base and the tube; and/or the second sealing ring is arranged between the pipe body and the cover body; and/or, the second sealing ring is arranged between the pipe bodies.

6. The separation and collection device of claim 1, wherein any two of the base, the tube, and the cover are removably connected.

7. The separation and collection device of claim 1, wherein the first filter and/or the second filter is a titanium dioxide membrane, an aluminum oxide membrane, a silicon pore membrane, or a polymeric membrane.

8. The separation and collection device of claim 1, further comprising a vibrator coupled to the base.

9. A collection system comprising a first peristaltic pump, a coarse filtration device and a separation and collection device according to any one of claims 1 to 8, wherein the output end of the first peristaltic pump is connected to the input end of the coarse filtration device, and the output end of the coarse filtration device is in communication with the first interface.

10. A collection method applied to the collection system of claim 9, the separation and collection device comprising a vibrator, comprising the steps of:

sequentially passing the mixed stock solution through the first peristaltic pump, the coarse filtering device, the first chamber, the second chamber and the third chamber;

driving an external circulation of the liquid in the first chamber by using the circulation driving assembly;

driving the liquid in the second cavity to circulate outside by using the circulation driving assembly;

driving the liquid in the second cavity to circulate outside by using the circulation driving assembly;

respectively obtaining target filtrate in the first chamber, the second chamber and the third chamber;

in the filtering process, the vibrator is utilized to drive the base to vibrate, so that the liquid in the first chamber, the second chamber and the third chamber vibrate.