CN117257842A - Five-cereal insect debridement bag, preparation method, system and application - Google Patents
Five-cereal insect debridement bag, preparation method, system and application Download PDFInfo
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- CN117257842A CN117257842A CN202311421145.4A CN202311421145A CN117257842A CN 117257842 A CN117257842 A CN 117257842A CN 202311421145 A CN202311421145 A CN 202311421145A CN 117257842 A CN117257842 A CN 117257842A
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/009—Sachets, pouches characterised by the material or function of the envelope
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Insects & Arthropods (AREA)
- Dermatology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention provides a five-cereal insect debridement bag, a preparation method, a system and application, and relates to the field of biomedical basic research. In the embodiment of the invention, fresh five-cereal insects can clean wounds by degrading, liquefying and only taking inactivated/necrotic tissues, promote wound healing, and can achieve ideal treatment effect on chronic wounds. The secretions and excretions of fresh five-cereal worms contain various bioactive substances including antibacterial peptides, anti-inflammatory factors, growth factors and the like. These substances can clear infection, reduce chronic inflammation, and accelerate the growth of new granulation tissue and blood vessels, thereby promoting wound healing. Through the functions of the aspects, the fresh five-cereal insects can effectively promote debridement, anti-infection and healing of wound surfaces, and a relatively natural and mild wound treatment method is provided for patients.
Description
Technical Field
The invention relates to the field of biomedical foundation research, in particular to a five-cereal insect debridement bag, a preparation method, a system and application.
Background
Wounds that fail to heal anatomically and functionally intact for more than 1 month are clinically defined as chronic wounds. With the acceleration of aging of the population, the proportion of chronic wound patients is continuously increased. The chronic wound surface brings a heavy economic burden to the medical care system and greatly reduces the quality of life of the affected population. Therefore, the medicine can treat chronic wound surface better and improve the life quality of patients.
Thus, a new chronic wound solution is currently needed.
Disclosure of Invention
In order to solve the problems in the background technology, the invention provides a five-cereal insect debridement bag, a preparation method, a system and application.
In a first aspect, the present invention provides a five-cereal insect debridement kit comprising: the dressing bag with micropores and fresh five-cereal insects are cultivated in a sterile environment and subjected to aseptic treatment to obtain the fresh five-cereal insects.
Optionally, the processing process of the fresh five-cereal insects comprises the following steps:
placing fresh five-cereal worms in 0.3% -2% iodophor solution under aseptic condition, standing for 5-40 min, and rinsing with sterile physiological saline containing 0.9% sodium chloride;
taking out the rinsed fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum, standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects;
taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent and/or a bactericide, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects.
Optionally, the five-cereal insect debridement bag further comprises: and (5) filling.
Optionally, the density of the fresh five-cereal insects in the five-cereal insect debridement bag is 5-30 strips/square centimeter.
In a second aspect, the invention provides a method for preparing a five-cereal insect debridement bag, which comprises the following steps:
placing fresh five-cereal worms in 0.3% -2% iodophor solution under aseptic condition, standing for 5-40 min, and rinsing with sterile physiological saline containing 0.9% sodium chloride;
taking out the rinsed fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum, standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects;
taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects;
transferring the sterile fresh five-cereal insects into a dressing bag under a sterile condition to obtain the five-cereal insect debridement bag.
Alternatively, the bacteriostatic agent is a nanoparticle of silver, or a mixture of nanoparticles of gold, silver, and platinum, or a solution containing 5-10% lactic acid, 5-10% sorbic acid, 6-10% anhydrous citric acid, 1-3% copper sulfate, 25-30% fatty acid glycerides, and 25-40% propylene glycol.
In a third aspect, the present invention provides a system for preparing a five cereal insect debridement bag, the system comprising:
the first sterile treatment device is used for placing fresh five-cereal worms in 0.3% -2% iodophor solution for standing for 5-40 minutes under a sterile condition, and then rinsing the fresh five-cereal worms with sterile physiological saline containing 0.9% sodium chloride;
the emptying treatment device is used for taking out the rinsed fresh five-cereal worms under the aseptic condition, placing the fresh five-cereal worms in aseptic animal milk or fetal bovine serum for standing for 5-40 minutes, and then rinsing the fresh five-cereal worms with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal worms;
the second aseptic processing device is used for taking out the fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects;
the preparation device is used for transferring the sterile fresh five-cereal insects into the dressing belt under the sterile condition to obtain the five-cereal insect debridement bag.
In a fourth aspect, the invention provides an application of fresh five-cereal insects in preparing a chronic wound debridement bag, wherein the fresh five-cereal insects are cultivated in a sterile environment and subjected to aseptic treatment, and the fresh five-cereal insects are used for cleaning wounds through degradation, liquefaction and ingestion of deactivated/necrotic tissues.
Optionally, the processing step of the fresh five-cereal worms comprises the following steps:
placing fresh five-cereal worms in 0.3% -2% iodophor solution under aseptic condition, standing for 5-40 min, and rinsing with sterile physiological saline containing 0.9% sodium chloride;
taking out the rinsed fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum, standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects;
taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects.
Optionally, the density of the fresh five-cereal insects is 5-30 strips/square centimeter.
The fresh five-cereal insects adopted in the five-cereal insect debridement bag provided by the embodiment of the invention are obtained by cultivating in a sterile environment and performing aseptic treatment, and are in line with clinical application. In the embodiment of the invention, the fresh five-cereal insects clean wounds by degrading, liquefying and only taking the inactivated/necrotic tissues, and meanwhile, secretions and excretions of the fresh five-cereal insects contain various bioactive substances including antibacterial peptides, anti-inflammatory factors, growth factors and the like. These substances can clear infection, reduce chronic inflammation, and accelerate the growth of new granulation tissue and blood vessels, thereby promoting wound healing. Through the functions of the aspects, the fresh five-cereal insects can effectively promote debridement, anti-infection and healing of wound surfaces, and a relatively natural and mild wound treatment method is provided for patients.
Drawings
Fig. 1 is a flowchart of a preparation method of a five-cereal insect debridement bag in an embodiment of the invention.
Detailed Description
The following examples are provided for a better understanding of the present invention and are not limited to the preferred embodiments described herein, but are not intended to limit the scope of the invention, any product which is the same or similar to the present invention, whether in light of the present teachings or in combination with other prior art features, falls within the scope of the present invention.
The specific experimental procedures or conditions are not noted in the examples and may be followed by the operations or conditions of conventional experimental procedures described in the literature in this field. The reagents used, as well as other instruments, are commercially available conventional reagent products, not identified to the manufacturer.
The five cereal insects are named as cereal insects, fagopyrum tataricum, fly maggots and maggots. The five-cereal insect is larvae of lucilia sericata, chrysomya megacephala or other closely related insects of the family of the blowflies. In ancient China, the forty volumes of the worm mesh of Ben Cao gang mu indicate that: "maggots and flies" are also used. For furuncle and ecthyma, it is ground into powder and mixed with sesame oil for external application. The fresh people can take salty-cold and detoxify peristaltic attack and dispersion more well. According to the description of Chinese medicine "Ben Cao Ji Yuan" and "Ben Cao Bian Ji Shen", the insect is dried and ground and then applied externally to treat ecthyma, lip furuncle, carbuncle and other infectious surgical diseases. All of the Chinese medicinal materials Zhi and northeast animal medicine are received by the names of five cereals.
For centuries, fresh five-cereal worms have been used to clean wounds by degrading, liquefying and only taking up the deactivated/necrotic tissue, and promote wound healing. This therapy is internationally known as biological debridement therapy (MDT). The fresh five-cereal worms are very effective in clinical treatment and are approved by the U.S. Food and Drug Administration (FDA) for medical use, and the use of the fresh five-cereal worms is also brought into the Liaoning province Chinese medicine processing Specification and the treatment standard of diabetic foot in China.
The existing fresh five-cereal worm processing standard is relatively simple and convenient, and the processing method comprises the following steps: the artificially cultivated larva of the five-cereal worm is washed. The invention provides a five-cereal insect debridement bag suitable for clinical application and a preparation method thereof without specific processing methods and processes.
Specifically, as shown in fig. 1, a flowchart of a method for preparing a five-cereal insect debridement bag in an embodiment of the present invention is shown, and the method for preparing a five-cereal insect debridement bag specifically includes:
s1, under the aseptic condition, placing fresh five-cereal worms in 0.3% -2% iodophor solution, standing for 5-40 minutes, and then rinsing by using sterile physiological saline containing 0.9% sodium chloride.
In the embodiment of the invention, the selected fresh five-cereal insects are larvae of lucilia sericata, chrysomya megacephala and adult flies of the family Leucophaceae. Under the aseptic condition, selecting artificially cultivated five-cereal insects or 1-3-year-old five-cereal insects after incubation of aseptic eggs, and taking the five-cereal insects with the length of 1mm-1.5cm as fresh five-cereal insects for subsequent treatment.
In the embodiment of the invention, the step S1 is repeated for 3 to 5 times, so that the preliminary disinfection of the fresh five-cereal insects can be finished.
S2, taking out the rinsed fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum for standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects.
In the embodiment of the invention, the fresh five-cereal insects after preliminary disinfection are treated according to the step S2 and repeated for 3-5 times, so that the intestinal contents of the fresh five-cereal insects can be emptied, the fresh five-cereal insects tend to be aseptic, and the method is suitable for clinical application.
S3, taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects.
In the embodiment of the present invention, in practical application, the step S3 may be repeated 3-5 times.
Alternatively, in an embodiment of the present invention, the bacteriostatic agent is a nanoparticle of silver, or the bacteriostatic agent is a mixture of nanoparticles of gold, silver and platinum, or the bacteriostatic agent is a solution containing 5-10% lactic acid, 5-10% sorbic acid, 6-10% citric acid anhydrous, 1-3% copper sulfate, 25-30% fatty acid glyceride, and 25-40% propylene glycol.
In the embodiment of the invention, during actual operation, any broad-spectrum bacteriostat can be adopted to perform bacteriostasis treatment on fresh five-cereal insects which empty intestinal contents.
S4, transferring the sterile fresh five-cereal insects into a dressing bag under a sterile condition to obtain the five-cereal insect debridement bag.
In order to facilitate clinical application, the embodiment of the invention provides that the sterile fresh five-cereal insects are transferred into the dressing bag under the sterile condition to obtain the five-cereal insect debridement bag.
In the embodiment of the invention, the material of the dressing bag can be selected from any one or more of the following, and in particular, different dressing bags can be designed according to the type and the requirement of the wound:
superabsorbent fibre dressing: has strong water absorption performance, can quickly absorb wound exudates, keep the wound dry and promote healing.
Collagen dressing: is rich in collagen, can provide good wound support and protection, and promotes tissue regeneration and healing.
Castor oil dressing: is rich in castor oil, has antibacterial, antiinflammatory, and moisturizing effects, and can promote wound healing and prevent infection.
Film dressing: the film dressing with the transparent texture can play a role of a physical barrier, prevent external pollution and bacterial infection, and has air permeability, thereby being beneficial to wound healing.
Gauze dressing: can absorb exudates, provide a protective covering, and at the same time be breathable, facilitating wound healing.
In the embodiment of the invention, the five-cereal insect debridement can be placed in a test tube containing sterile culture solution for storage and transportation.
In the embodiment of the invention, before the step S4, fresh five-cereal worms can be placed on the sterile absorbent paper to absorb surface moisture, and the vitality of the five-cereal worms is observed. Selecting the five-cereal worms with normal vitality to prepare the five-cereal worm debridement bag.
In practical application, after the steps S1 to S3 are completed, the obtained fresh five-cereal worms may be further inspected, which specifically includes: randomly selecting 100 fresh five-cereal worms, crushing to prepare homogenate, and performing bacterial inspection and virology inspection. Making qualified five-cereal insects in the same batch into a five-cereal insect debridement bag, or directly transferring into a sterile unidirectional ventilation culture bottle, and preserving at 4-30deg.C and relative humidity of 27% -85% for use.
The second aspect of the embodiment of the invention also provides a preparation system of the five-cereal insect debridement bag, which comprises:
the first sterile treatment device is used for placing fresh five-cereal worms in 0.3% -2% iodophor solution for standing for 5-40 minutes under a sterile condition, and then rinsing the fresh five-cereal worms with sterile physiological saline containing 0.9% sodium chloride;
the emptying treatment device is used for taking out the rinsed fresh five-cereal worms under the aseptic condition, placing the fresh five-cereal worms in aseptic animal milk or fetal bovine serum for standing for 5-40 minutes, and then rinsing the fresh five-cereal worms with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal worms;
the second aseptic processing device is used for taking out the fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects;
the preparation device is used for transferring the sterile fresh five-cereal insects into the dressing belt under the sterile condition to obtain the five-cereal insect debridement bag.
In the embodiment of the present invention, the preparation system corresponds to the preparation method provided in the steps S1 to S4, and the specific apparatus used to complete the preparation method may be a conventional culture apparatus, for example: sterile glass petri dishes.
A third aspect of the embodiments of the present invention provides a five-cereal insect debridement pack, the five-cereal insect debridement pack comprising: the dressing bag with micropores and fresh five-cereal insects are cultivated in a sterile environment and subjected to aseptic treatment to obtain the fresh five-cereal insects.
In the embodiment of the invention, the pore diameter of the micropores on the dressing bag can be determined according to the size of the fresh five-cereal insects, and the diameter of the micropores on the dressing bag is smaller than that of the fresh five-cereal insects.
Specifically, in the embodiment of the present invention, the fresh five-cereal worms may be obtained by the treatment in the steps S1 to S3.
Optionally, the five-cereal insect debridement bag further comprises: and (5) filling.
In the embodiment of the invention, the five-cereal insect debridement bag can also comprise a filler so as to maintain the temperature and the humidity in the debridement bag and further maintain the activity of the five-cereal insects.
Specifically, the filler may include any one or more of the following:
gauze (2): different thicknesses and sizes can be set according to different requirements so as to adapt to wounds of different sizes and types;
high absorbency sponge: such a filler has excellent water absorption properties, can rapidly absorb wound secretions, reduces the risk of infection, and a highly absorbent sponge is generally sterile to ensure a clean environment around the wound.
Collagen filler: collagen is a natural protein, which plays an important role in wound repair; collagen fillers can provide structural support and promote the healing process.
Transparent negative pressure sponge: the transparent negative pressure sponge is a filler with negative pressure function, can be used for wound surface closure and the formation of moist environment, can help to reduce infection rate and promote wound healing.
Hydrogel filler: the hydrogel filler has effects of keeping moisture and promoting healing, and can absorb wound secretion and maintain moist environment of wound, thereby promoting wound healing.
In the embodiment of the invention, the density of the fresh five-cereal insects in the five-cereal insect debridement bag is 5-30 pieces/square centimeter, and the fresh five-cereal insects are 1-3 years old fresh five-cereal insects. When the method is specifically applied, the wound surface of a patient can be covered by the five-cereal insect debridement, so that 5-30 fresh five-cereal insects with 1-3 ages are placed on the wound surface of the patient every square centimeter. In specific application, the wound can be disinfected by using the iodophor before using the five-cereal insect debridement bag, and is flushed by using normal saline, so that exudation of the chronic wound is eliminated, and meanwhile, the wound is kept moist.
In the embodiment of the invention, after the wound surface of the patient is covered by the wound debridement of the five-cereal insects, bandaging and fixing can be carried out by using a bandage.
When the method is specifically applied, the fresh five-cereal worms are used for 2-3 days each time, the wound surfaces of the patients are covered with the fresh five-cereal worms in a debridement mode all the time, then the wound surfaces are washed by using iodophor, the fresh five-cereal worms are taken out, and according to clinical requirements, the wound surfaces of the patients can be covered with the fresh five-cereal worms in a debridement mode.
The fourth aspect of the embodiment of the invention provides an application of fresh five-cereal insects in preparing a chronic wound debridement bag, wherein the fresh five-cereal insects are cultivated in a sterile environment and subjected to aseptic treatment, and the fresh five-cereal insects are used for cleaning wounds through degradation, liquefaction and ingestion of deactivated/necrotic tissues.
Optionally, the processing step of the fresh five-cereal worms comprises the following steps:
placing fresh five-cereal worms in 0.3% -2% iodophor solution under aseptic condition, standing for 5-40 min, and rinsing with sterile physiological saline containing 0.9% sodium chloride;
taking out the rinsed fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum, standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects;
taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects.
Optionally, the density of the fresh five-cereal insects is 5-30 strips/square centimeter.
In the embodiment of the invention, when the method is specifically applied, the fresh five-cereal worms can be clinically applied to preparing the five-cereal worm debridement bag, or the fresh five-cereal worms can be directly placed on the wound surface of a patient and then covered by dressing or gauze.
When the method is specifically applied, the wound surface of a patient can be observed every day, and a small amount of physiological saline is dripped into the dry part of the wound surface. If a large amount of liquid seeps out from the wound surface, the bandage wrapped by the outer layer is replaced.
When the five-cereal insect debridement bag is replaced in specific application, the debridement bag can be taken down by forceps and put into a test tube filled with 75% alcohol or chlorine-containing disinfectant for sealing.
In the embodiment of the invention, specific clinical application cases are also provided:
case 1: after 85 years old men have 30 years history of diabetes mellitus, the patients have complicated MRSA drug-resistant bacteria infection due to non-healing incision migration after toe interception of diabetic foot blood disorder, and the patients are rolled to 30 domestic hospitals for more than 10 years old, and the patients take more than 30 ten thousands of yuan, so that the treatment methods have no obvious effect. The external application treatment of the five cereal insect debridement bag is applied, the wound can be replaced every 3 days, after the wound is used for 2 weeks (about 2500 yuan), the infection is effectively controlled, the granulation of the wound surface is regenerated, the area of the wound surface is gradually reduced, and the wound surface is finally and completely healed.
Case 2: in 91-year-old men, the heel is pressed for a long time to cause huge defect wound surfaces, and the treatment method is combined with pseudomonas aeruginosa infection, takes hundreds of thousands of yuan, and has no obvious effect on various treatment methods. The wound surface is gradually reduced after the wound surface is changed every 3 days by applying the external application treatment of the five cereal insect debridement bag (about 8000 yuan is spent), the wound surface is filled with newly generated granulation, the bacteria is cultivated to turn into yin, and finally the wound surface is completely healed.
Case 3: in 41 years old men, severe aplastic anemia patients, venous drugs extravasate during hospitalization, leading to large-area necrotizing fasciitis on the palmar side and the dorsal side of the forearm. HB 47g/L; PLT 9 is 109/L, can not carry out operation debridement, is ineffective in conventional dressing change, and can effectively remove necrotic tissue after external application treatment of the five-cereal insect debridement bag, fresh granulation grows, and the wound surface is completely healed.
Case 4: in 53 years old men, 2 months after knee joint tibial plateau fracture operation, severe postoperative infection leads to exposure of the steel plate to the outside, and a large amount of wound surface seeps. If the traditional treatment mode is adopted, the steel plate is taken out by operation and debridement treatment is repeated for a plurality of times. But such fractures are difficult to heal and the estimated cost is 5-10 ten thousand yuan. The external application treatment of the five cereal insect debridement bag is applied for 3 weeks, 8 times of treatment are spent, 4800 yuan is spent, the wound surface is gradually reduced, exudation is obviously reduced, and the wound is finally healed perfectly.
Case 5: men aged 77 have a history of diabetes for 20 years. One year ago, the first toe of the right foot is blackened and is shrunken without obvious causes. It takes approximately 20 ten thousand yuan for the external hospital toe interception and vascular intervention treatment, but the incision after operation overflows pus and does not heal bacteria for a long time. The traditional Chinese medicine has various diseases such as heart failure, pleural effusion and the like. The external application treatment of the five cereal insect debridement bag is applied, the five cereal insect debridement bag is replaced every 3 days, the infection is controlled after the treatment is carried out for 4 weeks (about 6000 yuan is spent), the exudation is obviously reduced, and the five cereal insect debridement bag is finally healed completely.
For the purposes of simplicity of explanation, the methodologies are shown as a series of acts, but one of ordinary skill in the art will recognize that the present invention is not limited by the order of acts described, as some acts may, in accordance with the present invention, occur in other orders and concurrently. Further, those skilled in the art will recognize that the embodiments described in the specification are all of the preferred embodiments, and that the acts and components referred to are not necessarily required by the present invention.
The five-cereal insect debridement bag, the preparation method, the system and the application provided by the invention are described in detail, and specific examples are applied to illustrate the principle and the implementation mode of the invention, and the description of the examples is only used for helping to understand the method and the core idea of the invention; meanwhile, as those skilled in the art will have variations in the specific embodiments and application scope in accordance with the ideas of the present invention, the present description should not be construed as limiting the present invention in view of the above.
Claims (10)
1. The five-cereal insect debridement bag is characterized by comprising: the dressing bag with micropores and fresh five-cereal insects are cultivated in a sterile environment and subjected to aseptic treatment to obtain the fresh five-cereal insects.
2. The five-cereal insect debridement bag of claim 1, wherein the fresh five-cereal insect treatment process comprises:
placing fresh five-cereal worms in 0.3% -2% iodophor solution under aseptic condition, standing for 5-40 min, and rinsing with sterile physiological saline containing 0.9% sodium chloride;
taking out the rinsed fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum, standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects;
taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent and/or a bactericide, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects.
3. The five-cereal insect debridement bag of claim 1, further comprising: and (5) filling.
4. The five-cereal insect debridement bag of claim 1, wherein the density of fresh five-cereal insects in the five-cereal insect debridement bag is 5-30 bars/square centimeter.
5. The preparation method of the five-cereal insect debridement bag is characterized by comprising the following steps:
placing fresh five-cereal worms in 0.3% -2% iodophor solution under aseptic condition, standing for 5-40 min, and rinsing with sterile physiological saline containing 0.9% sodium chloride;
taking out the rinsed fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum, standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects;
taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects;
transferring the sterile fresh five-cereal insects into a dressing bag under a sterile condition to obtain the five-cereal insect debridement bag.
6. The method according to claim 5, wherein the bacteriostatic agent is silver nanoparticles, or the bacteriostatic agent is a mixture of gold, silver and platinum nanoparticles, or the bacteriostatic agent is a solution containing 5-10% lactic acid, 5-10% sorbic acid, 6-10% citric acid anhydrous, 1-3% copper sulfate, 25-30% fatty acid glyceride and 25-40% propylene glycol.
7. A system for preparing a five cereal insect debridement kit, the system comprising:
the first sterile treatment device is used for placing fresh five-cereal worms in 0.3% -2% iodophor solution for standing for 5-40 minutes under a sterile condition, and then rinsing the fresh five-cereal worms with sterile physiological saline containing 0.9% sodium chloride;
the emptying treatment device is used for taking out the rinsed fresh five-cereal worms under the aseptic condition, placing the fresh five-cereal worms in aseptic animal milk or fetal bovine serum for standing for 5-40 minutes, and then rinsing the fresh five-cereal worms with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal worms;
the second aseptic processing device is used for taking out the fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects;
the preparation device is used for transferring the sterile fresh five-cereal insects into the dressing belt under the sterile condition to obtain the five-cereal insect debridement bag.
8. The application of the fresh five-cereal insects in preparing the chronic wound debridement bag is characterized in that the fresh five-cereal insects are cultivated in a sterile environment and subjected to aseptic treatment, and the fresh five-cereal insects are used for cleaning wounds through degradation, liquefaction and ingestion of deactivated/necrotic tissues.
9. The use according to claim 8, wherein the step of treating the fresh five-cereal worms comprises:
placing fresh five-cereal worms in 0.3% -2% iodophor solution under aseptic condition, standing for 5-40 min, and rinsing with sterile physiological saline containing 0.9% sodium chloride;
taking out the rinsed fresh five-cereal insects under aseptic conditions, placing the fresh five-cereal insects in aseptic animal milk or fetal bovine serum, standing for 5-40 minutes, and rinsing the fresh five-cereal insects with aseptic physiological saline containing 0.9% sodium chloride to empty intestinal contents of the fresh five-cereal insects;
taking out the fresh five-cereal insects under the aseptic condition, placing the fresh five-cereal insects in aseptic normal saline containing a bacteriostatic agent, standing for 2-40 minutes, and then rinsing the fresh five-cereal insects with the aseptic normal saline containing 0.9% sodium chloride to obtain the aseptic fresh five-cereal insects.
10. The use according to claim 8, characterized in that the density of use of the fresh five-cereal insects is 5-30 bars/cm.
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| CN202311421145.4A CN117257842A (en) | 2023-10-30 | 2023-10-30 | Five-cereal insect debridement bag, preparation method, system and application |
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| CN202311421145.4A CN117257842A (en) | 2023-10-30 | 2023-10-30 | Five-cereal insect debridement bag, preparation method, system and application |
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