CN117233223A - Multichannel detection circuit, device and method - Google Patents
Multichannel detection circuit, device and method Download PDFInfo
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Abstract
The application relates to the technical field of chip detection, in particular to a multi-channel detection circuit, a device and a method, comprising a data acquisition unit, a detection unit and a detection unit, wherein the data acquisition unit comprises a processing module, a channel selection module and a first power module, the processing module is connected with the channel selection module, and the first power module is respectively connected with the processing module and the channel selection module; the interaction unit is respectively connected with the processing module and the first power supply module; the channel selection module comprises a transimpedance preamplifier circuit and a constant potential circuit connected with the transimpedance preamplifier circuit; the application can realize the function of single-channel connection multi-channel detection by the mutual coordination among the processing module, the channel selection module, the first power module and the interaction unit, and avoids the trouble that the multi-channel test can be realized only by changing different test interfaces in the test process.
Description
Technical Field
The application relates to the technical field of chip detection, in particular to a multi-channel detection circuit, a multi-channel detection device and a multi-channel detection method.
Background
The electrochemical workstation is divided into a single-channel workstation and a multi-channel workstation; the single-channel workstation can only test one sample at a time, and the multi-channel workstation is equivalent to a plurality of single-channel electrochemical workstations which are assembled together, so that the test of a plurality of samples can be simultaneously carried out, and the test efficiency is higher; the multichannel workstation is suitable for a scene requiring large-scale research and development test, and can remarkably accelerate the research and development speed; however, the electrochemical workstations on the market are mainly single-channel workstations, the testing equipment of the multi-channel workstations is not integrated, the volume is large, information transmission is disturbed, the multi-channel testing can be realized only by replacing different testing interfaces in the testing process, the testing process and the method are complex, and data acquired by multi-channel detection can be output only by transmitting the data to a computer.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.
The application has been developed in view of the above-mentioned or prior art problems in that a test procedure requires a different test interface to be replaced in order to achieve a multi-channel test.
It is therefore an object of the present application to provide a multi-channel detection circuit.
In order to solve the technical problems, the application provides the following technical scheme: a multi-channel detection circuit includes,
the data acquisition unit comprises a processing module, a channel selection module and a first power module, wherein the processing module is connected with the channel selection module, and the first power module is respectively connected with the processing module and the channel selection module;
and the interaction unit is respectively connected with the processing module and the first power supply module.
As a preferred embodiment of the multi-channel detection circuit of the present application, wherein: the channel selection module comprises a transimpedance preamplifier circuit and a constant potential circuit connected with the transimpedance preamplifier circuit;
and the transimpedance preamplifier circuit and the constant potential circuit are respectively connected with the first power supply module.
As a preferred embodiment of the multi-channel detection circuit of the present application, wherein: and the resistor network circuit of the transimpedance preamplifier circuit is connected with the processing module.
As a preferred embodiment of the multi-channel detection circuit of the present application, wherein: the transimpedance preamplifier circuit further comprises a reference electrode test port and a counter electrode test port;
and the counter electrode test port is connected to the reference electrode test port.
As a preferred embodiment of the multi-channel detection circuit of the present application, wherein: the interaction unit comprises a control module, a first USB interface and a second power module, and the control module is respectively connected with the processing module and the first power module through the first USB interface.
The multichannel detection circuit has the beneficial effects that: the application can realize the function of single-channel connection multi-channel detection by the mutual coordination among the processing module, the channel selection module, the first power module and the interaction unit, and avoids the trouble that the multi-channel test can be realized only by changing different test interfaces in the test process.
In view of the fact that in the practical use process, the multi-channel workstation testing equipment is not integrated, the size is large, and the information transmission is disturbed.
In order to solve the technical problems, the application also provides the following technical scheme: a multi-channel detection device comprises a multi-channel detection device, comprising the multi-channel detection circuit; the method comprises the steps of,
the bearing shell comprises a chip electrode clamping groove and a mounting groove, and the control module is embedded in the mounting groove;
the chip electrode connecting unit comprises a chip electrode connecting circuit board, and an electrode elastic sheet and a spring type connecting terminal which are respectively connected to two sides of the chip electrode connecting circuit board;
the reference electrode test port, the counter electrode test port and the working motor port of the constant potential circuit are all connected with the spring type wiring terminal;
the electrode spring plate is embedded in the protective shell of the chip electrode connecting unit, the chip electrode connecting circuit board and the spring type wiring terminal are arranged in the protective shell, and one end of the protective shell is embedded in and rotationally connected with the chip electrode clamping groove.
As a preferred embodiment of the multi-channel detection device of the present application, wherein: an accommodating space is arranged in the bearing shell;
wherein, the data acquisition unit set up in the accommodation space.
As a preferred embodiment of the multi-channel detection device of the present application, wherein: a chip to be tested is arranged in the chip electrode clamping groove;
the electrode spring plate is connected with the chip to be tested.
The multichannel detection device has the beneficial effects that: the application integrates the data acquisition unit, the interaction unit, the bearing shell and the chip electrode connection unit, thereby greatly reducing the detection volume, reducing the anti-interference problem of information transmission and improving the detection speed and accuracy.
In view of the fact that in the practical use process, a testing method also exists, and the data acquired by multi-channel detection needs to be transmitted to a calibration computer to be output.
In order to solve the technical problems, the application also provides the following technical scheme: a multi-channel detection method includes a multi-channel detection device, the multi-channel detection method includes steps of,
starting an interaction unit and setting an acquisition test mode;
connecting the chip electrode connecting module with a chip to be tested which is placed in the chip electrode clamping groove;
performing four-stage life cycle test on the chip to be tested to obtain acquisition test data;
and collecting test data, transmitting the test data to a control module, calculating the peak height, and drawing a peak height cycle number chart.
As a preferred embodiment of the multi-channel detection method of the present application, wherein: the four-stage lifecycle includes an acquisition interface lifecycle, a sensor interface lifecycle, a curve interface lifecycle, and a history number lifecycle.
The multichannel detection method has the beneficial effects that: the application combines the circuit and software operation to realize the electrochemical workstation Cyclic Voltammetry (CV) and Square Wave Voltammetry (SWV) of the chip electrode multichannel control, and realizes the high flux collection and data processing functions of the multichannel chip electrode by utilizing the processing module to import Square Wave Voltammetry (SWV) data for processing.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
fig. 1 is an overall schematic diagram of a multi-channel detection circuit.
Fig. 2 is a schematic diagram of a processing module of the multi-channel detection circuit.
Fig. 3 is a schematic diagram of a channel selection module of the multi-channel detection circuit.
Fig. 4 is a schematic diagram of a power supply display circuit of the multi-channel detection circuit.
Fig. 5 is a schematic diagram of a battery charge-discharge circuit of the multi-channel detection circuit.
Fig. 6 is a schematic diagram of a power monitoring circuit of the multi-channel detection circuit.
Fig. 7 is a schematic diagram of a voltage conversion circuit of the multi-channel detection circuit.
Fig. 8 is a schematic diagram of a serial-to-UAB circuit of the multi-channel detection circuit.
Fig. 9 is a schematic diagram of a buzzer circuit of the multi-channel detection circuit.
Fig. 10 is a schematic diagram of an acquisition connection display circuit of the multi-channel detection circuit.
Fig. 11 is an overall schematic diagram of a multi-channel detection device.
Fig. 12 is a schematic diagram of a chip electrode connection unit of the multi-channel detection device.
Fig. 13 is a schematic flow chart of a detection step of the multi-channel detection method.
Fig. 14 is a schematic view of an APP interface for a multi-channel detection method.
FIG. 15 is a schematic diagram of a menu bar-device interface for a multi-channel detection method.
FIG. 16 is a schematic diagram of a software page-menu bar-cyclic voltammetry mode setup interface for a multi-channel detection method.
FIG. 17 is a schematic diagram of a software page-menu bar-square wave voltammetry mode setup interface for a multi-channel detection method.
Fig. 18 is a schematic diagram of a software page-menu bar-import interface of the multi-channel detection method.
Detailed Description
In order that the above-recited objects, features and advantages of the present application will become more readily apparent, a more particular description of the application will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, but the present application may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present application is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the application. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Referring to fig. 1 and 2, a multi-channel detection circuit is provided for a first embodiment of the present application, which includes a data acquisition unit 100, implementing accurate acquisition and bottom calculation of a Working Electrode (WE)/a reference electrode (CE)/a counter electrode (RE), and simultaneously being used for transmitting acquired data with an interaction unit 200, where the data acquisition unit 100 includes a processing module 101, a channel selection module 102 and a first power module 103, the processing module 101 is capable of receiving an instruction signal sent by the interaction unit 200, executing detection by the channel selection module 102, and transmitting acquired chip data to be detected to the interaction unit 200, the channel selection module 102 provides multi-circuit paths such as a Working Electrode (WE), a reference electrode (CE), and a counter electrode (RE), implementing multiple channel gating functions of the Working Electrode (WE), the reference electrode (CE), and the counter electrode (RE), and the processing module 101 is connected with the channel selection module 102, and provides corresponding circuits for different types of chips to be conducted, implementing selective acquisition or sequential acquisition of multiple channels; the first power module 103 supplies power to the processing module 101 and the channel selection module 102, and the first power module 103 is connected with the processing module 101 and the channel selection module 102 through unidirectional power transmission respectively; the interaction unit 200 plays roles of starting a detection program, sending an instruction signal, receiving collected data and checking, and is respectively connected with the processing module 101 and the first power module 103, and specifically, the model of the processing module 101 is STM32H750.
Further, as shown in fig. 3, the channel selection module 102 includes a transimpedance preamplifier circuit 102a and a potentiostat circuit 102b connected to the transimpedance preamplifier circuit 102a, the transimpedance preamplifier circuit 102a provides a reference electrode (CE) and a counter electrode (RE) circuit path, the potentiostat circuit 102b provides a Working Electrode (WE) circuit path, and the transimpedance preamplifier circuit 102a and the potentiostat circuit 102b cooperate with each other to provide a multi-channel electrode selection acquisition or sequential alternate acquisition basis for a chip to be detected.
The transimpedance preamplifier circuit 102a is connected with the processing module 101, and the resistor network circuit 102a-1 of the transimpedance preamplifier circuit 102a is connected with the processing module 101, so that bidirectional information signal transmission for starting a collection reference electrode (CE) and a counter electrode (RE) circuit to collect data and transmit the collected data to the chip can be realized; specifically, pin 8 (D), pin 1 (A0), pin 16 (A1), and pin 15 (A2) of the single-chip microcomputer U9 of the transimpedance preamplifier circuit 102a are respectively connected with pin 55 (RE), pin 24 (PA 3), pin 20 (PA 1), and pin 22 (PA 2) of the processing module 101, respectively, and pin 8 (D), pin 1 (A0), pin 16 (A1), and pin 15 (A2) of the single-chip microcomputer U10 of the resistor network circuit 102a-1 are respectively connected with pin 54 (CE), pin 24 (PA 3), pin 20 (PA 1), and pin 22 (PA 2) of the processing module 101.
The constant potential circuit 102b is used for receiving a start acquisition signal of the transimpedance preamplifier circuit 102a, unidirectionally transmitting data of a Working Electrode (WE) circuit access acquisition chip to the processing module 101 and the transimpedance preamplifier circuit 102a, and unidirectionally transmitting the constant potential circuit 102b to the processing module 101; specifically, pin 8 (D), pin 1 (A0), pin 16 (A1), and pin 15 (A2) of the single-chip microcomputer U7 of the potentiostat circuit 102b are respectively connected to pin 53 (WE), pin 24 (PA 3), pin 20 (PA 1), and pin 22 (PA 2) of the processing module 101; it should be noted that, the models of the single-chip microcomputer U9, the single-chip microcomputer U10 and the single-chip microcomputer U7 are ADG1408YRUZ.
It is noted that the transimpedance preamplifier circuit 102a further includes a reference electrode test port 102a-2 and a counter electrode test port 102a-3; the counter electrode test port 102a-3 is connected to the counter electrode test port 102a-2 in a unidirectional manner to transmit data collected by the counter electrode, the potentiostatic circuit 102b includes 8 circuit channels (according to detection, not limited to 8), the counter electrode test port 102a-2 and the counter electrode test port 102a-3 are connected to the chip electrode connection circuit board through wires in an integrated manner, it should be noted that the counter electrode test port 102a-3, the counter electrode test port 102a-2 and the counter electrode test port 102a-3 are respectively provided with a pin 4 (S1), a pin 5 (S2), a pin 6 (S3), a pin 7 (S4), a pin 12 (S5), a pin 11 (S6), a pin 10 (S7) and a pin 9 (S8) respectively, and the 8 ports of the counter electrode test port 102a-2 and the counter electrode test port 102a-3 are respectively correspondingly connected to the 8 chip electrode connection circuit board through three ADG1408 yuz digital-analog converters, so that the collection of the electrodes of the micro-chip to be detected can be replaced for different chips (i.e. the chip to be detected, the detection efficiency of the micro-chip can be improved, and the detection of the detection module can be designed, and the detection of the chip is 8.
Further, the transimpedance preamplifier circuit 102a and the potentiostatic circuit 102b are respectively connected with the first power supply module 103; specifically, the first power module 103 includes a power supply display circuit 103a, a battery charge-discharge circuit 103b, a power monitoring circuit 103c, and a voltage conversion circuit 103d; specifically, as shown in fig. 4, the power supply display circuit 103a is configured to display whether external 12V power is normally connected (if connected, green light is on, if connected, green light is not on); as shown in fig. 5, the battery charge-discharge circuit 103B can charge, store and supply power to the voltage conversion circuit 103d, in which B1 and B6 are series-parallel connection of batteries, and Q1 to Q3 are insulated gate field effect transistor (MOSFET) circuits for balancing charge and discharge of the battery pack, so that charge balance between batteries is achieved; as shown in fig. 6, the power monitoring circuit 103c is a voltage divider circuit, and is used for collecting voltage for the ADC to determine the battery power, and the pin ADC-CH1 of the power monitoring circuit 103c is connected to the pin 18 (PA 0) of the processing module 101; as shown in fig. 7, the voltage conversion circuit 103D is used for stabilizing and converting voltages, so as to provide a direct current ±6v operational amplification analog power supply, +3.3v analog and digital power supplies, +5v and +5.5v digital power supplies required for detection and collection of the processing module 101 and the channel selection module 102, wherein the a+6v voltage input by the voltage conversion circuit 103D is respectively connected in parallel with the pin 7 (a+6v) and the pin 9 (a+6v) of the processing module 101, and the transimpedance preamplifier circuit 102a, the potentiostatic circuit 102b and the singlechip pin 2 (EN) and the pin 13 (VDD) of the resistor network circuit 102a-1, the a+6v voltage input by the voltage conversion circuit 103D is respectively connected with the pin 8 (a-6V), the pin 10 (a-6V) and the singlechip pin 3 (VSS) of the transimpedance preamplifier circuit 102a, the potentiostatic circuit 102b and the resistor network circuit 102a-1, and the voltage input by the voltage conversion circuit 103D is respectively connected with the pin 8 (a-6V), the singlechip pin 3V and the pin 3 (39) of the processing module 101 and the like; the 12V external power supply is connected to the power supply display circuit 103a, the battery charge/discharge circuit 103b, the power monitoring circuit 103c, and the voltage conversion circuit 103d, respectively.
Further, the interaction unit 200 includes a control module 201, a first USB interface 202 and a second power module 203, where the control module 201 is connected to the processing module 101 and the first power module 103 through the first USB interface 202, the second power module 203 is connected to an external circuit module, the control module 201 plays roles of sending a multi-channel acquisition signal, receiving acquisition data and calibrating and comparing the acquisition data, specifically may be touch display (performance: cortex A9,1GB DDR3,8GB eMMC,Android5.1), it is specifically man-machine interface software, supports chip sampling parameter editing, supports dynamic graphic display in a sampling process, supports real-time storage of data in a sampling process, supports table display of sampling data and the like, and the second power module 203 includes a 220C ac-to-12V dc power adapter, a 12V dc lithium battery (capacity 5000 ma/h) and a 12V dc circuit, and the 20C ac-to-12V dc power adapter is connected to the 12V dc circuit through the 12V dc lithium battery (capacity 5000 ma/h); the first USB interface 202 is connected to the serial port of the processing module 101 through the switching module 104 of the data acquisition unit 100, and the interface 202 is transmitted; specifically, the second USB interface 104a of the switching module 104 is respectively connected to the first USB interface 202, the serial-to-UAB circuit 104b and the first power module 103, where the serial-to-UAB circuit 104b is used to transmit the collected data to the processing module 101 and the control module 201 detects the collected communication interface, as shown in fig. 8, the pin NC and the pin CH-RXD of the serial-to-UAB circuit 104b are both connected to the pin 36 (PA 9) of the processing module 101, and the pin CH-TXD and the pin NC of the serial-to-UAB circuit 104b are both connected to the pin 38 (PA 10).
Further, the data acquisition unit 100 further includes a warning template 105, where the warning template 105 includes a buzzer circuit 105a and an acquisition connection display circuit 105b, the buzzer circuit 105a plays a role of acquiring a completion prompt sound, as shown in fig. 9, a pin of the buzzer circuit 105a is connected to a pin 48 (PA 15) of the processing module 101, and green, yellow and blue prompt lamps are disposed in the acquisition connection display circuit 105b, and when the chip to be detected is respectively connected to the working electrode test port 102b-1, the reference electrode test port 102a-2 and the counter electrode test port 102a-3, the green, yellow and blue prompt lamps are respectively illuminated to indicate that multiple channels are normally connected, specifically, as shown in fig. 10, the pin LED-SYS, the pin LED-CH1 and the pin LED-CH 2 of the acquisition connection display circuit 105b are respectively connected to a pin 45 (PC 13), a pin 47 (PC 14) and a pin 49 (PC 15) of the processing module 101.
Further, the main hardware parameter ranges of the data acquisition unit 100 are provided herein:
voltage range: 10V;
groove pressure: 10V (Max);
current range: 100 mA/+ -200 mA/+ -400 mA;
reference electrode input impedance: 1mΩ;
sensitivity range: 4X 10-8-0.1/0.2/0.4A total eight gear;
input bias current: <10pA;
current measurement resolution: <1pA;
and a data acquisition system: 16 bit sampling, maximum 100KHz;
and (3) power supply: DC12V/1.5A.
The application can realize the function of single-channel (single electrode) connection multi-channel detection by the mutual coordination among the processing module, the channel selection module, the first power module and the interaction unit, and avoids the trouble that the multi-channel test can be realized only by changing different test interfaces in the test process.
Example 2
Referring to fig. 11 to 12, in a second embodiment of the present application, unlike the previous embodiment, the present embodiment provides a multi-channel detection device, which solves the problems of non-integration, large volume and interference in information transmission of a multi-channel workstation test apparatus, and includes a multi-channel detection circuit; and, bear the weight of the body 300, including chip electrode card slot 301 and mounting groove 302, chip electrode card slot 301 offers the accommodation space for waiting to test the chip, the mounting groove 302 is used for controlling the module 201 to embed and install on the multichannel detection device, and then realize the integration of the detection equipment, control the module 201 to embed and set up in the mounting groove 302; the chip electrode connection unit 400 comprises a chip electrode connection circuit board 401, an electrode elastic sheet 402 and a spring type connecting terminal 403, wherein the electrode elastic sheet 402 and the spring type connecting terminal 403 are respectively welded on two sides of the chip electrode connection circuit board 401; the reference electrode test port 102a-2, the counter electrode test port 102a-3 and the working motor port 102b-1 of the potentiostat circuit 102b are all provided with wires and are welded with the spring-type wiring terminal 403, so that the detection of the multi-channel electrochemical chip (i.e. the chip to be detected) is realized, and the detection data can be directly processed through the device. Meanwhile, the device can also be provided with corresponding circuit conducting modules aiming at different types of chip electrodes, so that the selective collection or sequential alternate collection of the multi-channel electrodes is realized.
Specifically, the electrode elastic sheet 402 is used for being connected with a chip electrode circuit to be tested, the electrode elastic sheet 402 is embedded in the protective shell 404 of the chip electrode connecting unit 400, the chip electrode connecting circuit board 401 and the spring type wiring terminal 403 are arranged in the protective shell 404, the rotating shafts on two sides of one end of the protective shell 404 are embedded in the chip electrode clamping groove 301 to be connected through rotation, when detection and collection are performed, the protective shell 404 is rotated, and the electrode elastic sheet 402 is embedded in the measuring hole of the chip to be tested.
It should be noted that, a containing space is provided in the bearing housing 300, where the data acquisition unit 100, the first USB interface 202 and the second power module 203 are all disposed in the containing space, and the containing space of the bearing housing 300 plays a role in bearing and protecting the data acquisition unit 100, the first USB interface 202 and the second power module 203, so as to provide a foundation for integrated detection.
Further, a chip to be tested is arranged in the chip electrode clamping groove 301; the electrode elastic sheet 402 is connected with a chip to be tested, specifically, the chip electrode card slot 301 is provided with 1 card slot, the chip to be tested is clamped in the card slot, the chip to be tested is an eight-channel chip containing 8 electrodes, and the elastic sheet of the electrode elastic sheet 402 is in contact connection with a measurement pin of the chip to be tested.
The chip electrode connection unit 400 may be modified according to different types of electrodes, and may be connected to a chip electrode, or may change the electrode connection module into a common electrode clip for connection of a common electrode (non-chip electrode), and its connection mode does not affect the program use.
The application integrates the data acquisition unit, the interaction unit, the bearing shell and the chip electrode connection unit, thereby greatly reducing the detection volume, reducing the anti-interference problem of information transmission and improving the detection speed and accuracy.
Example 3
Referring to fig. 13, in a third embodiment of the present application, unlike the previous embodiment, this embodiment provides a multi-channel detection method, including a multi-channel detection device, the steps of the multi-channel detection method include: starting an interaction unit 200 and setting an acquisition test mode; connecting the chip electrode connection unit 400 with the chip to be tested placed in the chip electrode card slot 301; performing four-stage life cycle test on the chip to be tested to obtain acquisition test data; the collected test data is transmitted to the control module 201 to calculate the peak height and map the peak height versus cycle number.
Further, the step of starting the interaction unit 200 and setting the collection test mode includes:
opening a switch of the control module 201 of the interaction unit 200;
starting a multichannel detection APP of the control module 201;
starting a main program;
creating a main program;
binding the interface to the description page;
initializing a USB authorization state to be unknown;
initializing a broadcast receiver to monitor USB insertion;
binding state controls.
It should be noted that, as shown in fig. 14, the multi-channel detection APP software mainly sets a menu bar, a real-time data selection frame, and a status bar; wherein the menu bar includes: devices, modes, start, stop, calibrate, export, import, and reference; specifically, as shown in fig. 15, the device menu bar is used to display whether the device is normally connected to the electrochemical workstation, i.e., the data acquisition unit 100; two electrochemical detection methods are selected in the mode menu bar, namely 2 modes are selectable, as shown in fig. 16 and 17, and relevant detection parameters including a Range (Range), an initial voltage (Init E), a highest voltage (High E), a lowest voltage (Low E), a Scan Rate (Scan Rate), a static Time (quick Time), a sampling interval (Sample Int), a Scan number (Sweep Segment) and an electrode Channel selection (Channel Switch) can be set in a Cyclic Voltammetry (CV) selection page; the square wave voltammetry selection page may set relevant detection parameters including Range (Range), start voltage (Init E), end voltage (Final E), delta voltage (Incr E), amplitude (Amplitude), frequency (Frequency), static Time (quick Time), cycle number (sylcle Time), cycle Interval (Cycle Interval), cycle Start electrode Channel selection (Start Channel), and Cycle End electrode Channel selection (End Channel); the start menu bar is used for controlling the detection start; the stop menu bar is used for controlling detection to stop; the calibration menu bar is used for detecting state calibration; the export menu bar is used for exporting the current detection result data; the input menu bar is used for inputting square wave voltammetry data, after the input, the peak height can be directly calculated according to the square wave voltammetry real-time detection of each channel, and a peak height-cycle number chart is drawn, as shown in fig. 18; importing a menu bar to record the basic performance of the software; the interface is also provided with a real-time data selection frame, the data which is being detected is displayed when the data is checked, and a result diagram of the imported data is displayed when the data is not checked; the software interface also sets a status bar including Stop status display (Stop), out-of-range display (Overflow), and state of charge (SOC).
Further, the chip electrode connection unit 400 is connected with the chip to be tested placed in the chip electrode card slot 301 by inserting the chip electrode connection unit 400 into the electrode spring plate 402 of the chip electrode connection unit 400.
Further, the step of performing a four-stage lifecycle test on the chip to be tested to obtain the collected test data includes:
starting a timing task of an automatic refreshing status bar;
a main program path state;
the main program is restored and initialized;
registering a broadcast receiver;
detecting whether a chip to be tested is connected or not;
connecting a chip to be tested;
starting to run the main program after connection;
carrying out four-stage life cycle test on the chip to be tested;
the buzzer circuit 105 sends out warning sound and logs out the broadcast receiver;
acquiring acquisition test data.
It should be noted that, if the chip to be detected is connected and is provided with green, yellow and blue indicator lamps in the acquisition and connection display circuit 105b, when the chip to be detected is respectively connected with the working electrode test port 102b-1, the reference electrode test port 102a-2 and the counter electrode test port 102a-3, the green, yellow and blue indicator lamps will be respectively on to indicate that the multiple channels are normally connected; if the green, yellow and blue lamps are not on or are not on, inquiring the unknown or authorized state of the USB, and if yes, connecting the chip to be detected; if not, the main program starts to run.
It should be noted that, the four-stage lifecycle includes an acquisition interface lifecycle, a sensor interface lifecycle, a curve interface lifecycle, and a history number lifecycle, and the acquisition interface lifecycle, the sensor interface lifecycle, the curve interface lifecycle, and the history number lifecycle are tested by adopting a program automatic inter-cutting mode, which can be implemented by editing program codes.
It should be noted that after the broadcast receiver is logged off, the test process may be ended, or the chip to be tested may be tested in four-stage life cycle.
Further, the step of collecting test data and transmitting the test data to the control module 201 to calculate the peak height and draw a peak height-cycle number chart includes:
stopping the test program and disconnecting the chip to be tested;
the collected test data are transmitted to an APP data display interface of the control module 201 through the switching module 104 and the first USB interface 202;
square wave voltammetry data are imported;
and calculating the peak height according to the square wave volt-ampere real-time detection of each channel and drawing a peak height-cycle number graph.
The Square Wave Voltammetry (SWV) diagram detected in real time can directly output a detection result through importing data, and the function of outputting the detection result to the chip electrode is realized.
The method comprises the following steps of selecting and collecting chips to be tested or collecting the chips in turn: the square wave voltammetry at the mode menu bar selects the detection parameters related to the page setting, wherein the Range of Range (Range), the starting voltage (Init E), the end voltage (Final E), the static Time (quill Time) are the same as the meaning and setting Range in the cyclic voltammetry parameter setting, and furthermore the delta voltage (Incr E): the step value of the potential in the process from the initial potential to the termination potential is input by a keyboard, and the setting range is 1-1000 mV; amplitude (Amplitude): representing the difference value of the adjacent two stable potentials, wherein the parameter setting range is 1-1000 mV; frequency (Frequency) pulse Frequency, the parameter setting range is 1-500'; number of cycles (Sycle Times): the square wave voltammetry scanning times from the initial potential to the final potential of each electrode channel are referred to, and the scanning is counted as one cycle from the initial electrode channel to the final electrode channel; cycle Interval (Cycle Interval): the time interval from the start electrode channel to the next electrode channel is set in seconds; cycle Start electrode Channel selection (Start Channel): a first electrode channel for cyclic scanning by square wave voltammetry; cycle termination electrode Channel selection (End Channel): square wave voltammetry is used for circularly scanning the last electrode channel. If the cycle start electrode channel selection and the cycle end electrode channel selection are set to the same electrode channel, the program will cycle scan the electrode channel at cycle intervals.
When the cyclic voltammetry interface is selected in the mode menu bar, "select collect" means select a designated Channel to collect in the "Channel Switch" bar. After selection, the instructions of the touch screen are transmitted to the ARM processor through the USB interface, so that the ARM processor controls the ADG1408YRUZ digital-to-analog converter to select one of the eight channels to be a channel.
After the square wave voltammetry interface is selected, the principle of 'selecting and collecting' is the same as that of cyclic voltammetry, and 'sequentially and alternately collecting' is that after the circulation times, the circulation interval time, the circulation start electrode channel selection and the circulation end electrode channel selection are set, instructions of the touch screen are transmitted to an ARM processor through a USB interface, and the ARM processor controls an ADG1408YRUZ digital-analog converter to sequentially and alternately open the selected channels according to the instructions and according to time intervals.
The application realizes the electrochemical workstation Cyclic Voltammetry (CV) and Square Wave Voltammetry (SWV) of the chip electrode multichannel control by combining the circuit and software operation, and realizes the high flux acquisition and data processing functions of the multichannel chip electrode by processing the imported Square Wave Voltammetry (SWV) data by utilizing the processing module 101. And secondly, a new detection mode is provided for a high-flux chip data acquisition mode through the design of a chip electrode connection module.
It is important to note that the construction and arrangement of the application as shown in the various exemplary embodiments is illustrative only. Although only a few embodiments have been described in detail in this disclosure, those skilled in the art who review this disclosure will readily appreciate that many modifications are possible (e.g., variations in sizes, dimensions, structures, shapes and proportions of the various elements, values of parameters (e.g., temperature, pressure, etc.), mounting arrangements, use of materials, colors, orientations, etc.) without materially departing from the novel teachings and advantages of the subject matter described in this application. For example, elements shown as integrally formed may be constructed of multiple parts or elements, the position of elements may be reversed or otherwise varied, and the nature or number of discrete elements or positions may be altered or varied. Accordingly, all such modifications are intended to be included within the scope of present application. The order or sequence of any process or method steps may be varied or re-sequenced according to alternative embodiments. In the claims, any means-plus-function clause is intended to cover the structures described herein as performing the recited function and not only structural equivalents but also equivalent structures. Other substitutions, modifications, changes and omissions may be made in the design, operating conditions and arrangement of the exemplary embodiments without departing from the scope of the present applications. Therefore, the application is not limited to the specific embodiments, but extends to various modifications that nevertheless fall within the scope of the appended claims.
Furthermore, in order to provide a concise description of the exemplary embodiments, all features of an actual implementation may not be described (i.e., those not associated with the best mode presently contemplated for carrying out the application, or those not associated with practicing the application).
It should be appreciated that in the development of any such actual implementation, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort might be complex and time consuming, but would nevertheless be a routine undertaking of design, fabrication, and manufacture for those of ordinary skill having the benefit of this disclosure.
It should be noted that the above embodiments are only for illustrating the technical solution of the present application and not for limiting the same, and although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present application may be modified or substituted without departing from the spirit and scope of the technical solution of the present application, which is intended to be covered in the scope of the claims of the present application.
Claims (10)
1. A multi-channel detection circuit, characterized by: comprising the steps of (a) a step of,
the data acquisition unit (100) comprises a processing module (101), a channel selection module (102) and a first power module (103), wherein the processing module (101) is connected with the channel selection module (102), and the first power module (103) is respectively connected with the processing module (101) and the channel selection module (102);
and the interaction unit (200) is respectively connected with the processing module (101) and the first power supply module (103).
2. The multi-channel detection circuit of claim 1, wherein: the channel selection module (102) comprises a transimpedance preamplifier circuit (102 a) and a constant potential circuit (102 b) connected with the transimpedance preamplifier circuit (102 a);
wherein the transimpedance preamplifier circuit (102 a) and the potentiostatic circuit (102 b) are respectively connected with the first power supply module (103).
3. The multi-channel detection circuit of claim 2, wherein: the resistor network circuit (102 a-1) of the transimpedance preamplifier circuit (102 a) is connected to the processing module (101).
4. A multi-channel detection circuit as claimed in claim 3, wherein: the transimpedance preamplifier circuit (102 a) further comprises a reference electrode test port (102 a-2) and a counter electrode test port (102 a-3);
wherein the counter electrode test port (102 a-3) is connected to the reference electrode test port (102 a-2).
5. The multi-channel detection circuit of claim 4, wherein: the interaction unit (200) comprises a control module (201), a first USB interface (202) and a second power module (203), wherein the control module (201) is respectively connected with the processing module (101) and the first power module (103) through the first USB interface (202).
6. A multi-channel assay device, characterized in that: comprising a multi-channel detection circuit as claimed in claims 1 to 5; the method comprises the steps of,
the bearing shell (300) comprises a chip electrode clamping groove (301) and a mounting groove (302), and the control module (201) is embedded in the mounting groove (302);
the chip electrode connecting unit (400) comprises a chip electrode connecting circuit board (401), electrode shrapnel (402) and a spring type connecting terminal (403) which are respectively connected to two sides of the chip electrode connecting circuit board (401);
wherein the reference electrode test port (102 a-2), the counter electrode test port (102 a-3) and the working motor port (102 b-1) of the potentiostatic circuit (102 b) are all connected with the spring type wiring terminal (403);
the electrode spring plate (402) is embedded in a protective shell (404) of the chip electrode connection unit (400), the chip electrode connection circuit board (401) and the spring type wiring terminal (403) are arranged in the protective shell (404), and one end of the protective shell (404) is embedded in and rotationally connected with the chip electrode clamping groove (301).
7. The multi-channel detector of claim 6, wherein: an accommodating space is arranged in the bearing shell (300);
wherein, the data acquisition unit (100) is arranged in the accommodating space.
8. The multi-channel assay device of claim 7, wherein: a chip to be tested is arranged in the chip electrode clamping groove (301);
the electrode spring piece (402) is connected with the chip to be tested.
9. A detection method using the multi-channel detection device according to any one of claims 6 to 8, characterized in that: comprises the steps of,
starting an interaction unit (200) and setting an acquisition test mode;
connecting the chip electrode connecting unit (400) with a chip to be tested placed in the chip electrode clamping groove (301);
performing four-stage life cycle test on the chip to be tested to obtain acquisition test data;
the collected test data are transmitted to a control module (201) to calculate the peak height and draw a peak height-cycle number graph.
10. The multi-channel detection method of claim 8, wherein: the four-stage lifecycle includes an acquisition interface lifecycle, a sensor interface lifecycle, a curve interface lifecycle, and a history number lifecycle.
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