CN117233094A

CN117233094A - Detection assembly and detection device

Info

Publication number: CN117233094A
Application number: CN202210633749.4A
Authority: CN
Inventors: 许一太
Original assignee: SAE Magnetics HK Ltd
Current assignee: SAE Magnetics HK Ltd
Priority date: 2022-06-06
Filing date: 2022-06-06
Publication date: 2023-12-15

Abstract

The application provides a detection assembly and a detection device. The detection assembly comprises a runner layer and an optical waveguide structure. On the one hand, a micro-channel (a millimeter-sized or even micrometer-sized channel) for guiding a sample to be detected is formed by utilizing the channel layer; on the other hand, the optical waveguide structure forms at least two optical waveguide paths, and a mixed region corresponding to each optical waveguide path is formed on the surface of the optical waveguide material of the optical waveguide structure. The micro flow channels direct the sample flow to pass through each mixing zone such that the sample is contacted with a different reactive fluid in the mixing zone. The light transmitted through the optical waveguide path passes through the corresponding mixing area in the transmission process, and the light is influenced by whether the reaction between the sample to be detected and the reaction fluid occurs or not when passing through different mixing areas, so that the intensity and/or the phase of the light are changed, and therefore, specific components corresponding to the reaction components in the sample to be detected can be qualitatively and/or quantitatively detected.

Description

Detection assembly and detection device

Technical Field

The present application relates to a detection device combining an optical waveguide structure and a micro flow channel for detecting a specific component in a sample, and particularly relates to a detection assembly and a detection device including the detection assembly.

Background

In the optical field, it is found through research that light is incident from an optically dense medium to an optically sparse medium at a certain incident angle, and when the incident angle is larger than a certain angle, a total reflection phenomenon occurs, the refracted light disappears, and the light propagates only in the reflection direction. At the position where the light ray is totally reflected, an evanescent wave propagating along the direction of the optically sparse medium is generated. Further, the evanescent wave is sensitively affected by the boundary reaction at the surface of the optical waveguide material during the propagation of light in the optical waveguide path. Thus, the above phenomenon can be utilized to accurately and qualitatively and/or quantitatively detect a specific component or component variation of a sample at the surface of the optical waveguide material by detecting the intensity variation or phase variation of the incident light and the outgoing light before and after being affected by the above.

Disclosure of Invention

The present application has been made in view of the above state of the art. It is an object of the present application to provide a detection assembly that enables a qualitative and/or quantitative accurate detection of a specific component in a sample to be detected (e.g. a biological sample) using the characteristics of the evanescent wave as described above. Another object of the present application is to provide a detection apparatus including the above-described detection assembly, which is capable of achieving a miniaturized structure in the case of qualitatively and/or quantitatively accurately detecting a specific component in a sample to be detected.

In order to achieve the above object, the present application adopts the following technical scheme.

The application provides a detection assembly, comprising:

a cap layer formed with separate inflow and outflow holes;

a flow channel layer formed with a micro flow channel communicating with the inflow hole and the outflow hole; and

and an optical waveguide structure forming at least two optical waveguide paths, mixing regions corresponding to each optical waveguide path being formed on a surface of an optical waveguide material of the optical waveguide structure, the micro flow channels being for guiding a sample to be inspected to flow from the inflow hole toward the outflow hole and through each mixing region, so that the sample to be inspected is in contact with a reaction fluid flowing through or stored in the mixing region, and light propagating through the optical waveguide paths passing through the corresponding mixing regions during propagation.

In an alternative, the flow channel layer is located between the cover layer and the optical waveguide structure in the thickness direction of the detection assembly, and the flow channel layer abuts against the cover layer and the optical waveguide structure.

In another alternative, the cover layer is located at one side of the flow channel layer in the thickness direction, the optical waveguide structure is located at the other side of the flow channel layer in the thickness direction, a surface of one side of the flow channel layer in the thickness direction is abutted with a surface of the other side of the cover layer in the thickness direction, and a surface of the other side of the flow channel layer in the thickness direction is abutted with a surface of one side of the optical waveguide structure in the thickness direction.

In another alternative, at least a part of the micro flow channel is formed on a surface of one side in the thickness direction of the flow channel layer.

In another alternative, the other portions of the micro flow channel than the portions communicating with the inflow holes and the outflow holes are closed by the cover layer from one side in the thickness direction.

In another alternative, the optical waveguide structure includes an upper cladding layer, a core, and a lower cladding layer, the core being made of the optical waveguide material and being located between the upper cladding layer and the lower cladding layer, the upper cladding layer abutting the runner layer.

In another alternative, the mixing region is located in the upper cladding layer.

In another alternative, the detection assembly includes a reactive fluid carrier disposed on the core, the reactive fluid carrier defining the mixing region.

In another alternative, the cap layer and the reaction fluid carrier face each other via the micro flow channel at a distance in the thickness direction of the detection assembly.

The application also provides a detection device which comprises the detection assembly according to any one of the technical schemes.

The application also provides a detection assembly comprising:

a cap layer formed with a separate inflow hole and outflow hole, the cap layer further formed with a storage part storing a reaction fluid;

an optical waveguide structure forming at least two optical waveguide paths, a mixing region corresponding to each optical waveguide path being formed on a surface of an optical waveguide material of the optical waveguide structure, the micro flow channel being for guiding a sample to be inspected to flow from the inflow hole toward the outflow hole and through each mixing region, each mixing region being in controlled communication with a corresponding storage portion such that the reaction fluid can flow from the storage portion to the corresponding mixing region in a controlled manner, such that the sample to be inspected is in contact with the reaction fluid at the mixing region, and light propagating through the optical waveguide path passing through the corresponding mixing region during propagation.

In another alternative, the storage portions and the corresponding mixing regions are opposed to each other across the flow channel layer, and the storage portions can be in controlled communication with the mixing regions via the micro flow channels.

In another alternative, the storage part includes a groove, and a bottom of the groove is formed with a through hole communicating with the micro flow channel and opposite to the corresponding mixing region, and the through hole can be controllably closed and opened.

In another alternative, the cover layer includes a main body portion and a cover portion assembled together, the storage portion is formed in the main body portion, the storage portion is formed with an opening that is open toward one side in the thickness direction, and the cover portion closes the opening.

In another alternative, the inflow hole and the outflow hole are formed in the main body portion and penetrate the main body portion in the thickness direction,

the main body part is also provided with a drainage groove connected with the inflow hole and used for guiding the sample to be detected into the inflow hole.

In another alternative, the micro flow channel includes a mixing through hole penetrating the flow channel layer in the thickness direction, and the reservoir may communicate with the mixing region via the mixing through hole.

In another alternative, the optical waveguide structure includes an upper cladding layer, a core, and a lower cladding layer, the core being made of the optical waveguide material and being located between the upper cladding layer and the lower cladding layer, the upper cladding layer abutting the runner layer, the mixing region being located in the upper cladding layer.

In another alternative, the reaction fluid comprises a buffer and a modifying pigment.

The application also provides a detection assembly comprising:

a cap layer formed with separate inflow and outflow holes;

a flow channel layer formed with a micro flow channel communicating with the inflow hole and the outflow hole;

an optical waveguide structure forming at least two optical waveguide paths, a mixing region corresponding to each optical waveguide path being formed on a surface of an optical waveguide material of the optical waveguide structure, the micro flow channel being for guiding a sample to be inspected to flow from the inflow hole toward the outflow hole and through each mixing region such that the sample to be inspected is in contact with a reaction fluid flowing through or stored in the mixing region, light propagating through the optical waveguide path passing through the corresponding mixing region during propagation; and

An optical system for capturing light so that the light is incident into each of the optical waveguide paths, and for guiding out the light in the optical waveguide paths from the optical waveguide paths.

In an alternative, the optical system includes a first optical device and a second optical device corresponding to each of the optical waveguide paths, the first optical device being configured to capture light from a light source to make the light incident into the corresponding optical waveguide path, the second optical device being configured to guide the light in the optical waveguide path out of the optical waveguide path,

the mixing region is located between the first optical device and the second optical device in an extending direction of the optical waveguide path.

In another alternative, the first optical device is one or more of a grating, a mirror, a lens group, a lens array, and a beam splitter; and/or

The second optical device is one or a combination of a plurality of gratings, reflectors, lenses, lens groups, lens arrays and beam splitters.

In another alternative, the detection assembly further includes only one light source, and light from the light source is made incident into all of the optical waveguide paths using the optical system.

In another alternative, the detection assembly further comprises a light detection portion, the light source and the light detection portion being disposed offset relative to the core.

In another alternative, the light source and the light detecting section are disposed on the same side of the core in a thickness direction of the detecting member; or alternatively

The light source and the light detecting portion are disposed on different sides of the core in a thickness direction of the detecting member.

In another alternative, the detection assembly further includes a light detection portion, and the light source and the light detection portion are disposed in alignment with opposite end faces of the core and are disposed on both end sides of the core of the optical waveguide structure, respectively.

In another alternative, the detection assembly further comprises a light detection portion, the light source comprises a self-luminescent film disposed on a surface of the core, and the light detection portion comprises a light detection film disposed on a surface of the core.

The application also provides a detection assembly comprising:

a cap layer formed with separate inflow and outflow holes;

an optical waveguide structure forming at least two optical waveguide paths, mixing regions corresponding to each of the optical waveguide paths being formed on a surface of an optical waveguide material of the optical waveguide structure, the micro flow channels being for guiding a sample to be inspected to flow from the inflow hole toward the outflow hole and through each of the mixing regions so that the sample to be inspected is in contact with a reaction fluid flowing through or stored in the mixing region, light propagating through the optical waveguide paths passing through the corresponding mixing region during propagation,

the optical waveguide structure comprises an upper cladding layer, a first core, a second core and a lower cladding layer, wherein the upper cladding layer is in butt joint with the runner layer, the first core and the second core are made of optical waveguide materials and are located between the upper cladding layer and the lower cladding layer, the second core is arranged on the first core, and the mixing area is arranged on the surface of the second core.

In an alternative, the second core has a refractive index of light greater than that of the first core, and the light is incident into the first core before entering the second core through the mixing region and then returning to the first core.

In another alternative, the detection assembly further includes a reactive fluid carrier disposed on a surface of the second core, the reactive fluid carrier defining the mixing region.

In another alternative, the cap layer includes a storage part for storing the reaction fluid, and the through holes of the bottom of the storage part are opposite to each other via the micro flow channel and the mixing region.

In another alternative, in the direction of the flow of the sample to be tested along the micro flow channel, the micro flow channel comprises an inflow part, a drainage part, an inflow branch part, a mixing through hole, an outflow branch part and an outflow part which are communicated in sequence,

the inflow portion is communicated with the inflow hole, the outflow portion is communicated with the outflow hole, the drainage portion and the inflow branch portion are used for guiding the sample to be detected to the mixing through hole, the mixing area is communicated with the mixing through hole, and the outflow branch portion is used for guiding the sample in the mixing through hole to the outflow portion.

In another alternative, the drainage portion includes a curved section and a straight section, the curved section being located between and in direct communication with both the inflow portion and the straight section, the curved section forming a reciprocally folded shape during extension from the inflow portion toward the straight section.

In another alternative, the micro flow channel further comprises a flow blocking structure provided at the outflow branch part, the outflow branch part is formed as a groove, and the flow blocking structure comprises a structure for changing the depth and/or width of the groove.

In another alternative, the cover layer is located on one side of the flow path layer in the thickness direction of the detection assembly, and closes the drainage portion, the inflow branch portion, the mixing through hole, and the outflow branch portion from the one side in the thickness direction.

In another alternative, the lower cladding layer includes a base layer made of glass and a cladding layer made of silica and covering the base layer, the cladding layer abutting the upper cladding layer.

By adopting the technical scheme, the application provides a detection assembly and a detection device. The detection assembly comprises a runner layer and an optical waveguide structure. On the one hand, a micro-channel (a millimeter-sized or even micrometer-sized channel) for guiding a sample to be detected is formed by utilizing the channel layer; on the other hand, the optical waveguide structure forms at least two optical waveguide paths, and a mixed region corresponding to each optical waveguide path is formed on the surface of the optical waveguide material of the optical waveguide structure. In this way, it is possible to flow a reaction fluid containing a reaction component capable of reacting with a specific component in a sample to be inspected through or stored in a corresponding mixing region, and further, the micro flow channel guides the sample to be inspected through each mixing region so that the sample to be inspected is brought into contact with a different reaction fluid in the mixing region. Further, the light propagating through the optical waveguide path passes through the corresponding mixing region in the propagation process, and when the light passes through different mixing regions, the light is influenced by whether the reaction occurs between the sample to be detected and the reaction fluid, so that the intensity and/or the phase of the light are changed, and therefore, specific components corresponding to the reaction components in the sample to be detected can be qualitatively and/or quantitatively detected. Moreover, the combination of the micro-channel and the optical waveguide structure is beneficial to simplifying the structure of the detection assembly and the detection device comprising the detection assembly, thereby achieving the purpose of miniaturization.

Drawings

Fig. 1A and 1B are explanatory diagrams for explaining a detection principle of a detection assembly according to the present application.

Fig. 1C-1I show illustrative schematic diagrams of alternatives of the detection assembly of the present application.

Fig. 2A is a perspective view showing a partial structure of a detection assembly according to a first embodiment of the present application, in which a cover layer is omitted in order to show a specific configuration of a micro flow channel and a mixing region.

Fig. 2B is a schematic top view showing the structure of the detection assembly in fig. 2A.

Fig. 2C is an exploded view showing the structure of the detection assembly in fig. 2A.

Fig. 2D is another exploded view illustrating the detection assembly of fig. 2A.

Fig. 2E is a schematic perspective view showing the structure of a cap layer of the detection assembly in fig. 2A.

Fig. 2F is a schematic cross-sectional view showing the cap layer of fig. 2E, with cross-hatching omitted.

Fig. 2G is a perspective view showing the structure of the flow channel layer of the detection assembly in fig. 2A.

Fig. 2H and 2I are perspective views showing the structure of the upper cladding layer of the detection assembly in fig. 2A.

Fig. 2J is a perspective view showing the structure of the first core of the detection assembly in fig. 2A.

Fig. 2K is a schematic perspective view showing a combination of both the second core of the detection assembly in fig. 2A and a reaction fluid carrier.

FIG. 2L is a schematic perspective view showing the structure of the base layer of the lower cladding layer of the detection assembly of FIG. 2A.

Fig. 2M is a perspective view showing a structure of a cladding of a lower cladding of the detection assembly in fig. 2A.

Fig. 2N is a perspective view showing an example structure of an entrance grating and an exit grating of the detection assembly in fig. 2A.

Fig. 3A is a perspective view showing a partial structure of a detection assembly according to a second embodiment of the present application, in which a cover layer is omitted in order to show a specific configuration of a micro flow channel and a mixing region.

Fig. 3B is a schematic top view showing the structure of the detection assembly in fig. 3A.

Fig. 3C is a perspective view showing the structure of the flow channel layer of the detection assembly in fig. 3A.

Fig. 4A is a perspective view showing a partial structure of a detection assembly according to a third embodiment of the present application, in which a cover layer is omitted in order to show a specific configuration of a micro flow channel and a mixing region.

Fig. 4B is a schematic top view showing the structure of the detection assembly in fig. 4A.

Fig. 4C is a perspective view showing the structure of the flow path layer of the detection assembly in fig. 4A.

Description of the reference numerals

LS light source; an LTS optical system; an OWG optical waveguide structure; OWP an optical waveguide path; an SR mixing region; an LR light detection unit;

1, a cover layer; 11a main body portion; 111 drainage grooves; 112 flow into the aperture; 113A first storage section; 113B a second storage section; 114 outflow holes; 12 cover parts;

2, a runner layer; 21 an inflow portion; 22 drainage parts; 221 a bending section; 222 straight line segments; 23A first inflow branch section; 23B second inflow branch section; 24A first mixing through hole; 24B second mixing through holes; 25A first outflow branch; 25B second outflow branch; 26. 26', 26 "flow blocking structure; 27 outflow portions; 28 grating holes;

3 an optical waveguide structure; 31 upper cladding; 311A first mixing tank; 311B second mixing tank; 312A first elongated slot; 312B second elongated slot; 313 grating grooves; 32 a first core; 33 a second core; 34 lower cladding; 341 a base layer; 342 coating;

4a reaction fluid carrier;

5A incidence grating; 5B, emitting a grating;

l length direction; w is the width direction; t thickness direction.

Detailed Description

Exemplary embodiments of the present application are described below with reference to the accompanying drawings. It should be understood that these specific illustrations are for the purpose of illustrating how one skilled in the art may practice the application, and are not intended to be exhaustive of all of the possible ways of practicing the application, nor to limit the scope of the application.

The optical principle utilized by the detection assembly according to the application is first described below. As explained in the background art, during the propagation of light in an optical waveguide path, an evanescent wave is sensitively affected by a boundary reaction at the surface of the optical waveguide path. With the above phenomenon, the inventors of the present application have made an accurate qualitative and/or quantitative detection of a specific component of a sample to be inspected, which flows through a mixing region and is capable of reacting with the specific reaction component, by disposing the reaction fluid including the specific reaction component at the mixing region at the surface of the optical waveguide material forming the optical waveguide path. In addition, in order to smoothly flow the sample to be inspected through the mixing region and in consideration of the need to miniaturize the detection assembly, the inventors of the present application also applied a micro flow channel (a flow channel of millimeter order or even micrometer order) to the detection assembly to drain the sample to be inspected.

Specifically, in an alternative of the detection assembly of the present application, as shown in fig. 1A, light from the same light source LS is transmitted through two optical waveguide paths OWP formed of an optical waveguide structure OWG (which may be typically a rectangular optical waveguide structure or a ridge optical waveguide structure) by using an optical system LTS, whereby parameters such as wavelengths of light passing through the two optical waveguide paths OWP are the same. The optical system LTS herein may be a combination of one or more of optical devices such as gratings, mirrors, lenses, lens groups, lens arrays, splitters, and the like. In these two optical waveguide paths OWP, a mixed region SR is provided on the surface of the optical waveguide material of the optical waveguide structure OWG, and a reaction fluid containing a reaction component is caused to flow through or be stored in one mixed region SR, while a reaction fluid not containing a reaction component is caused to flow through or be stored in the other mixed region SR. In the technical scheme of the application, the micro flow channel is utilized to drain the sample to be detected and enable the sample to be detected to flow through the two mixing areas SR at the same time, and specific components of the sample to be detected can react with reaction components, so that the intensity and/or the phase of light rays transmitted in the optical waveguide path OWP provided with the mixing areas SR are significantly changed due to the influence of the reaction. Other parameters in the two optical waveguide paths OWP are identical except for whether the reactive components are contained. In this way, the values of the parameters such as the intensity and/or the phase of the light beam measured by the optical waveguide path (alignment path) OWP provided with the one mixing region SR can be used as the alignment data, and the values of the parameters such as the intensity and/or the phase of the light beam measured by the optical waveguide path (reference path) OWP provided with the other mixing region SR can be used as the reference data. Thus, by comparing the data with the reference data (performing processing such as denoising), the specific component of the sample to be detected in the mixed region SR is accurately detected qualitatively and/or quantitatively. It will be appreciated that the optical system LTS shown in fig. 1A may include a beam splitter and a mirror to enable light rays of the same light source LS to propagate through the two optical waveguide paths OWP, respectively.

When it is desired to perform qualitative and/or quantitative accurate detection of a plurality of parameters in a sample to be detected, as shown in fig. 1B, in an alternative embodiment of the detection assembly of the present application, in addition to the optical waveguide paths OWP for acquiring reference data, a reaction fluid containing different reaction components may be stored in or caused to flow through the mixing region SR in correspondence with the other plurality (three are shown in the drawing) of optical waveguide paths OWP, thereby enabling detection of different specific components in the sample to be detected. In the above embodiments, the components of the corresponding reaction fluid in each optical waveguide OWP are identical except for the reaction components. Of course, in order to more accurately detect a specific component in a sample to be detected, the same reaction fluid containing a reaction component corresponding to the specific component may be stored or flowed in the mixing region SR corresponding to the plurality of optical waveguide paths OWP. It will be appreciated that the optical system LTS in fig. 1B may include a diffraction grating and a lens array to achieve propagation of light rays of the same light source LS through four optical waveguide paths OWP, respectively. In addition, the reference path and the comparison path may be provided in pairs, that is, two pairs of the reference path and the comparison path are provided in fig. 1B, so that the detection of two specific parameters is achieved.

In addition, in order to adapt to different application scenarios and improve the flexibility of the structural layout of the detection assembly, the kind and the installation position of the light source LS, the composition and the installation position of the optical system LTS, and the like may be adjusted. Specifically, as shown in fig. 1C, in an alternative of the detection assembly of the present application, the light source LS and the light detection portion LR may be disposed offset with respect to the optical waveguide structure OWG and disposed laterally of the optical waveguide structure OWG, for example, disposed on the upper side in fig. 1C. In this way, as shown in fig. 1C, the light from the light source LS is captured from one side of the optical waveguide structure OWG and is incident into the optical waveguide structure OWG, propagates in the optical waveguide path defined in the optical waveguide structure OWG by total reflection, and causes a change in intensity and/or phase due to the influence of the evanescent wave in the mixed region SR, and is finally detected by the light detecting section LR. Further, when the light source LS, the light detecting portion LR, and the light waveguide structure OWG are arranged in the manner shown in fig. 1C, the function of capturing the light from the light source LS to transmit into the light waveguide structure OWG and transmitting the light in the light waveguide structure OWG to the light detecting portion LR may be realized using the grating shown in fig. 1D. The same function may also be achieved with one or more of the prisms shown in fig. 1E and the optical devices such as the polarizing plate shown in fig. 1F. In the above alternatives, the light source LS and the light detection portion LR may be arranged offset with respect to the optical waveguide structure OWG and arranged on the same side of the optical waveguide structure OWG, it being understood that in other alternatives the light source LS and the light detection portion LR may be arranged offset with respect to the optical waveguide structure OWG and arranged on different sides of the optical waveguide structure OWG. Further, as shown in fig. 1G, in an alternative of the detection assembly of the present application, the light source LS and the light detection portion LR may be disposed to be aligned with the end faces of the optical waveguide structure OWG, and disposed at both end sides of the optical waveguide structure OWG. In this case, as shown in fig. 1H, light from the light source LS may be incident on the optical waveguide structure OWG by an optical device such as a polarizing plate.

Further, regarding the kind of the light source LS and the associated arrangement position, in the alternative shown in fig. 1C to 1H, the detection assembly of the present application may typically employ an LD (laser diode) or an LED (light emitting diode) or the like, which is external to the optical waveguide structure OWG, as the light source LS, and correspondingly may typically employ a PD (photoelectric sensor), a CCD (charge coupled device), a CMOS (complementary metal oxide semiconductor), a power meter or the like, which is external to the optical waveguide structure OWG, as the light detection section LR. In the alternative shown in fig. 1I, the detection module of the present application may further employ an organic self-luminous film (which may be formed of an OLED, i.e., an organic light emitting semiconductor) directly provided on the optical waveguide material of the optical waveguide structure OWG forming the optical waveguide path, and the organic self-luminous film may be made to emit light by external excitation, and correspondingly may employ a light detection film directly provided on the optical waveguide material of the optical waveguide structure OWG as the light detection portion LR.

Further, regarding the micro flow channel, in different detection components, the drainage of the sample to be detected can be achieved by adopting micro flow channels with different structures. In addition, in order to prevent the sample to be inspected from being contaminated in the course of flowing through the micro flow channel and in view of easy manufacturing, a configuration in which the micro flow channel is formed in the flow channel layer and the portions of the micro flow channel other than the inflow portion and the outflow portion are closed with the cap layer may be adopted in the detection assembly of the present application. With respect to the flow channel layer and the cover layer, specific examples may be found in the embodiments of the detection assembly of the present application described below.

The detection assembly of the present application can be constructed using the above optical principles and technical ideas, and the structure of the detection assembly according to the first embodiment of the present application will be described below with reference to the drawings of the specification.

In an embodiment of the application, the detection assembly is integrally formed in a generally rectangular parallelepiped shape. "longitudinal direction", "width direction" and "thickness direction" refer to the longitudinal direction, the width direction and the thickness direction, respectively, of the detection assembly according to the present application, in which light is conducted in the form of total reflection in the optical waveguide path formed by the optical waveguide structure, and it can be considered that the light propagates in the optical waveguide path as a whole in the longitudinal direction, that is, the extending direction of the optical waveguide path is the longitudinal direction, which is also the extending direction of the core formed of the optical waveguide material in the optical waveguide structure.

In an embodiment of the present application, the "flow direction" refers to a flow direction of a sample to be tested (e.g., blood) in a micro flow channel of a flow channel layer of a detection assembly, wherein the flow direction is correspondingly restricted with a specific configuration of each portion of the micro flow channel.

(construction of the detection Assembly according to the first embodiment of the application)

As shown in fig. 2A to 2D, the detection assembly according to the first embodiment of the present application includes a cap layer 1, a runner layer 2, an optical waveguide structure 3, and an optical system that are assembled together. The cap layer 1, the runner layer 2, and the optical waveguide structure 3 are arranged in a stacked manner from one side to the other side in the thickness direction T. Assuming that the surface of each portion facing one side in the thickness direction T is a first surface and the surface of each portion facing the other side in the thickness direction T is a second surface, the first surface of the cap layer 1 is exposed toward one side in the thickness direction T, the second surface of the cap layer 1 is in contact with the first surface of the runner layer 2 (in particular, the two surfaces are in contact with each other, and the second surface of the runner layer 2 is in contact with the first surface of the optical waveguide structure 3, and the second surface of the optical waveguide structure 3 is exposed toward the other side in the thickness direction T.

The structure of each component of the detection unit will be described below.

(cover layer)

In the present embodiment, as shown in fig. 2E and 2F, the cap layer 1 includes a main body portion 11 and a cap portion 12 assembled together.

As shown in fig. 2E, the body 11 is formed with an inflow hole 112 and an outflow hole 114 penetrating in the thickness direction T. The inflow hole 112 is opposed to the inflow portion 21 described below of the micro flow channel of the flow channel layer 2 in the thickness direction T, and the outflow hole 114 is opposed to the outflow portion 27 described below of the micro flow channel of the flow channel layer 2 in the thickness direction T, so that the sample to be inspected, which flows in via the inflow hole 112, can flow into the inflow portion 21 of the micro flow channel along the inflow hole 112, and after the sample to be inspected has passed through the micro flow channel through the whole course, flows out from the outflow portion 27 via the outflow hole 114, and the outflow hole 114 can be externally connected to a suction device, which can be used to promote the flow of the sample to be inspected in the micro flow channel.

Further, as shown in fig. 2E, the first surface of the main body 11 is also formed with a drainage groove 111 connected to the inflow hole 112. The drainage groove 111 extends linearly along the longitudinal direction L, and the drainage groove 111 is recessed toward the other side in the thickness direction T. After the sample to be inspected is added to the drainage groove 111, the drainage groove 111 is used for draining the sample to be inspected into the inflow hole 112.

Further, as shown in fig. 2E, the first surface of the main body 11 is also formed with a first storage portion 113A and a second storage portion 113B corresponding to the reaction fluid carrier 4 provided in the optical waveguide structure 3. The first storage portion 113A and the second storage portion 113B are each formed as a groove recessed toward the other side in the thickness direction T, the first storage portion 113A and the second storage portion 113B being spaced apart from each other, the first storage portion 113A corresponding to the first optical waveguide path of the optical waveguide structure 3, and the second storage portion 113B corresponding to the second optical waveguide path of the optical waveguide structure 3. The first storage portion 113A stores a reaction fluid containing a reaction component (for example, aldehyde), and the second storage portion 113B stores a reaction fluid containing no reaction component. Further, taking the first storage portion 113A as an example, as shown in fig. 2F, a bottom portion of the first storage portion 113A may be formed with a through hole that is opposed to the mixing region defined by the reaction fluid carrier 4 provided in the first optical waveguide path. With the through-holes open, the reaction fluid can flow into the mixing region. The second storage portion 113B is formed with a corresponding structure, and the second storage portion 113B faces the mixing region defined by the reaction fluid carrier 4 provided in the second optical waveguide path. Further, the two cover portions 12 are respectively covered on the first storage portion 113A and the second storage portion 113B. When the detection assembly is not used, the protruding structures formed by the cover portion 12 close the through holes at the bottoms of the first storage portion 113A and the second storage portion 113B, respectively; when the detection assembly is used, the cover 12 may be lifted, pivoted by a corresponding mechanical mechanism (e.g., spring, lever, etc.), or the cover 12 may be directly removed so that the reaction fluid flows onto the reaction fluid carrier 4 on the optical waveguide path. In an alternative, the through holes, which are the bottoms of the grooves of the storage portions 113A, 113B, are closed by a protective film, and the cover portion 12 has a protruding structure disposed opposite to or partially inserted into the through holes, and pressing the cover portion 12 can cause the protruding structure to break the protective film so that the reaction fluid in the storage portion flows to the mixing region defined by the reaction fluid carrier 4. In addition, fine holes may be formed in the cover 12 so that the internal pressure of the storage portions 113A, 113B is the same as the atmospheric pressure after the cover 12 is lifted, thereby allowing the reaction fluid to smoothly flow out through the through holes.

(flow passage layer)

In the present embodiment, as shown in fig. 2A to 2D and 2G, a micro flow channel of a predetermined shape is formed in the flow channel layer 2, and on the one hand, the micro flow channel is capable of guiding a sample to be inspected to a mixing region defined by the reaction fluid carrier 4, and further guiding the sample to be inspected from the mixing region; on the other hand, the micro-flow channel can filter foreign matters in the sample to be detected to a certain extent, and accuracy of detection results is improved.

The runner layer 2 may be formed of a polymer. As shown in fig. 2A to 2D and 2G, a micro flow channel of a predetermined shape is formed on the first surface of the flow channel layer 2, and is used for draining a sample to be inspected entering the micro flow channel. In the flow direction of the sample to be inspected, the micro flow path includes an inflow portion 21, a drainage portion 22, an inflow branching portion (a first inflow branching portion 23A and a second inflow branching portion 23B), a mixing through hole (a first mixing through hole 24A and a second mixing through hole 24B), an outflow branching portion (a first outflow branching portion 25A and a second outflow branching portion 25B), a flow blocking structure 26, and an outflow portion 27, which are sequentially communicated.

The inflow portion 21 is formed as a blind hole and has a circular cross-sectional shape. The opening of the inflow portion 21 is opposed to the inflow hole 112 of the cap layer 1, and the opening size of the inflow portion 21 should be ensured so that the sample to be inspected can smoothly flow from the inflow hole 112 of the cap layer 1.

The drainage portion 22 is formed to penetrate the flow path layer 2 in the thickness direction T. One end of the drainage portion 22 communicates with the inflow portion 21, and the other end of the drainage portion 22 communicates with the inflow branching portions 23A, 23B. In the present embodiment, the drainage portion 22 includes a bent section 221 and a straight section 222. The bending section 221 is located between the inflow portion 21 and the straight section 222 and is in direct communication with both the inflow portion 21 and the straight section 222, and the bending section 221 is formed in a shape that is reciprocally folded back in the width direction W in the process of extending from the inflow portion 21 toward the straight section 222. The bending section 221 is formed into a smoothly transiting curved shape during bending, not into a bent shape having a sharp included angle. In this way, the pressure drop of the sample to be inspected is low in the process of smoothly guiding the sample to be inspected flowing from the inflow portion 21 to the straight line segment 222 by the bending segment 221. Furthermore, when the sample to be tested contains a plurality of components to be mixed, the bending section 221 facilitates the components to be mixed sufficiently. The straight line segment 222 is used to communicate the bent segment 221 with the inflow branching portions 23A, 23B. The straight line segment 222 extends linearly along the length direction L, and the straight line segment 222 extends through between the two grating holes 28 corresponding to the incident grating 5A.

The inflow branching portions 23A, 23B are formed to penetrate the runner layer 2 in the thickness direction T. The inflow branching portions 23A, 23B include a first inflow branching portion 23A and a second inflow branching portion 23B, and the first inflow branching portion 23A and the second inflow branching portion 23B each communicate with the straight line section 222 of the drainage portion 22. The first inflow branching portion 23A and the second inflow branching portion 23B extend obliquely with respect to the straight line section 222 so that the three are formed in an inverted "Y" shape in a plan view shown in fig. 2B, thereby constituting a Y-shaped micro flow channel. The first inflow branching portion 23A is for smoothly draining a part of the sample to be inspected to the first mixing through hole 24A, and the second inflow branching portion 23B is for smoothly draining another part of the sample to be inspected to the second mixing through hole 24B.

The first mixing through hole 24A and the second mixing through hole 24B are each formed to penetrate the runner layer 2 in the thickness direction T. The runner layer 2 and the under cladding 34 surround the reaction cell at the locations where the first mixing through hole 24A and the second mixing through hole 24B are located. The first optical waveguide path corresponds to one reaction cell in which a mixing region is defined mainly by the reactive fluid carrier 4, and a portion of the first core 32 and the second core 33 forming the first optical waveguide path and the corresponding reactive fluid carrier 4 are exposed through the first mixing through-hole 24A. The second optical waveguide path corresponds to another reaction cell in which a mixing region is defined mainly by the reactive fluid carrier 4, and a portion of the first core 32 and the second core 33 forming the second optical waveguide path and the corresponding reactive fluid carrier 4 are exposed through the second mixing through-hole 24B. Further, the first mixing through holes 24A communicate with the first inflow branching portions 23A so that the sample to be inspected flowing in via the first inflow branching portions 23A can flow into the mixing regions defined by the reaction fluid carriers 4 of the corresponding reaction cells. The second mixing through holes 24B communicate with the second inflow branching portions 23B so that the sample to be inspected flowing in via the second inflow branching portions 23B can flow into the mixing regions defined by the reaction fluid carriers 4 of the corresponding reaction cells.

The outflow branch portions 25A, 25B are formed in a groove shape recessed toward the other side in the thickness direction T and do not penetrate the flow channel layer 2 in the thickness direction T. The outflow branch portions 25A, 25B include a first outflow branch portion 25A and a second outflow branch portion 25B, the first outflow branch portion 25A communicates with the first mixing through hole 24A, and the second outflow branch portion 25B communicates with the second mixing through hole 24B. In order to leave the sample to be examined in the reaction cell for as long as possible, flow blocking structures 26 may be formed in both the first outflow branch portion 25A and the second outflow branch portion 25B. As shown in fig. 2B and 2G, in the present embodiment, on the one hand, the flow blocking structure 26 includes a plurality of steps formed at the bottom of the first outflow branch portion 25A and a plurality of steps formed at the bottom of the second outflow branch portion 25B; on the other hand, the flow blocking structure 26 further includes a configuration in which the bottom of the first outflow branch portion 25A and the bottom of the second outflow branch portion 25B are gradually raised toward the outflow portion. The steps and the gradually-rising structures form a height difference structure in the thickness direction T, and the flow of the sample to be detected from the reaction tank can be delayed as much as possible in the process of draining the sample to be detected through the micro-channel, so that the sample to be detected and the reaction fluid can be fully mixed, and further specific components in the sample to be detected and reaction components in the reaction fluid can be fully reacted.

The outflow portion 27 is formed as a blind hole and has a circular cross-sectional shape. The outflow portion 27 communicates with both the first outflow branch portion 25A and the second outflow branch portion 25B, so that the sample to be inspected via the first outflow branch portion 25A and the second outflow branch portion 25B merges into the outflow portion 27. In addition, the opening of the outflow portion 27 is opposed to the outflow hole 114 of the cap layer 1, and the size of the opening of the outflow portion 27 should be such that the sample to be inspected can smoothly flow into the outflow hole.

It is understood that, in the above-described flow path layer 2, the depth of the inflow portion 21 and the outflow portion 27 in the thickness direction T is the shallowest, and the depth of the drainage portion 22, the inflow branch portions 23A and 23B, and the mixing through holes 24A and 24B is the deepest due to penetration through the flow path layer 2, and the depth of the outflow branch portions 25A and 25B in the thickness direction T becomes progressively shallower from the mixing through holes 24A and 24B toward the outflow portion 27. Further, by adopting the above-described flow path layer 2, the sample to be inspected, which has entered from the inflow portion 21, can be caused to flow in two flow paths, one of which corresponds to the first optical waveguide, specifically, the inflow portion 21→the drainage portion 22→the first inflow branching portion 23a→the first mixing through hole (communicating with the mixing region) 24a→the first outflow branching portion 25a→the outflow portion 27; the other flow path corresponds to the second optical waveguide, specifically, the inflow portion 21→the drainage portion 22→the second inflow branching portion 23b→the second mixing through hole (communicating with the mixing region) 24b→the second outflow branching portion 25b→the outflow portion 27. In one flow path, the sample to be inspected reacts with the reaction components of the reaction fluid in the reaction cell formed by the first mixing through hole 24A; in the other flow path, the sample to be inspected is mixed with the reaction fluid containing no reaction component only in the reaction cell formed by the second mixing through hole 24B and cannot react.

In the flow path layer 2, two grating holes 28 for exposing the incident grating 5A are formed at both sides of the straight line segment 222 of the flow guiding portion 22 in the width direction W, and two grating holes 28 for exposing the exit grating 5B are formed at both sides of the outflow portion 27 in the width direction W. In the width direction W, one grating hole 28 for the incident grating 5A is provided on one side of the straight line section 222 of the drainage portion 22, and one grating hole 28 for the exit grating 5B is provided on one side of the outflow portion 27, and the incident grating 5A and the exit grating 5B corresponding to the two grating holes 28 are used in pairs. In the width direction W, the other grating hole 28 for the incident grating 5A is provided on the other side of the straight line section 222 of the drainage portion 22, the other grating hole 28 for the exit grating 5B is provided on the other side of the outflow portion 27, and the incident grating 5A and the exit grating 5B corresponding to the two grating holes 28 are used in pairs.

(optical waveguide structure)

In the present embodiment, as shown in fig. 2A to 2D, the optical waveguide structure 3 includes an upper clad 31, a first core 32, a second core 33, and a lower clad 34 for forming two optical waveguide paths. The light from the same light source can be propagated through total reflection in the two optical waveguide paths by using the two groups of gratings 5A and 5B.

As shown in fig. 2H and 2I, on the first face of the upper cladding layer 31, the upper cladding layer 31 is formed with two grating grooves 313 corresponding to the grating holes 28 for the incident grating 5A, respectively, and these grating grooves 313 are identical in shape and size to the corresponding grating holes 28, and the grating grooves 313 and the grating holes 28 together form a receiving cavity that receives the incident grating 5A. Further, on the upper side surface of the upper cladding layer 31, the upper cladding layer 31 is further formed with two grating grooves 313 corresponding to the grating holes 28 for the exit grating 5B, respectively, and the shape and size of these grating grooves 313 are the same as those of the corresponding grating holes 28, and the grating grooves 313 and the grating holes 28 together form a housing chamber housing the exit grating 5B.

Further, on the first surface of the upper cladding layer 31, the upper cladding layer 31 is also formed with a first mixing groove 311A corresponding to the first mixing through hole 24A and a second mixing groove 311B corresponding to the second mixing through hole 24B. The first mixing groove 311A has the same shape and size as the first mixing through hole 24A, and the first mixing groove 311A and the first mixing through hole 24A together form a reaction tank accommodating the second core 33 and the reaction fluid carrier 4. The second mixing groove 311B has the same shape and size as the second mixing through hole 24B, and the second mixing groove 311B and the second mixing through hole 24B together form a reaction tank accommodating the second core 33 and the reaction fluid carrier 4.

Further, on the second surface of the upper cladding layer 31, the upper cladding layer 31 is formed with two first and second elongated grooves 312A and 312B parallel to each other, the first and second elongated grooves 312A and 312B extending linearly along the length direction L and each having a rectangular cross-sectional shape. The shape and size of the first slot 312A and the second slot 312B correspond to the shape and size of the first core 32. In the thickness direction T of the upper cladding layer 31, the first elongated groove 312A communicates with one grating groove 313 for the incident grating 5A, one grating groove 313 for the exit grating 5B, and the first mixed groove 311A, and the second elongated groove 312B communicates with the other grating groove 313 for the incident grating 5A, the other grating groove 313 for the exit grating 5B, and the second mixed groove 311B.

In the present embodiment, as shown in fig. 2A to 2D, the lower cladding layer 34 includes a base layer 341 made of glass and a cladding layer 342 made of silica and covering the base layer 341. The lower cladding layer 34 is located on the lower side of the upper cladding layer 31 in the thickness direction T. The cladding 342 of the lower cladding 34 and the upper cladding 31 surround each other by the first elongated groove 312A and the second elongated groove 312B to form a receiving cavity for receiving the first core 32, and the upper cladding 342 is in contact with the upper cladding 31 in the thickness direction T. Typical shapes of the base layer 341 and the cover layer 342 are shown in fig. 2L and 2M, respectively.

In the present embodiment, as shown in fig. 2J, the first core 32 is made of an optical waveguide material and is formed in a long strip shape extending linearly along the length direction L. The two first cores 32 are inserted into the accommodation chamber formed by the upper cladding 31 and the lower cladding 34 using the long grooves 312A, 312B. One first core 32 of the two first cores 32 extends through one grating groove 313 for the incidence grating 5A, the first mixing groove 311A, and one grating groove 313 for the exit grating 5B, and the first core 32 is exposed via one grating groove 313 for the incidence grating 5A, the first mixing groove 311A, and one grating groove 313 for the exit grating 5B. The other first core 32 of the two first cores 32 extends through the other grating groove 313 for the incidence grating 5A, the second mixing groove 311B, and the other grating groove 313 for the exit grating 5B, and the first core 32 is exposed via the other grating groove 313 for the incidence grating 5A, the second mixing groove 311B, and the other grating groove 313 for the exit grating 5B.

In the present embodiment, as shown in fig. 2K, the second core 33 is made of an optical waveguide material and is formed in a long strip shape extending linearly along the length direction L. The optical waveguide material of the second core 33 is different from the optical waveguide material of the first core 32, the refractive index of the optical waveguide material of the second core 33 to light is larger than the refractive index of the optical waveguide material of the first core 32 to light, and the second core 32 is disposed at an intermediate position of the first core 33 and between the corresponding incident grating 5A and the exit grating 5B. It will be appreciated that the dimension of the first core 32 in the length direction L is primarily related to the dimension of the entire detection assembly in the length direction L, and that the dimension of the second core 33 in the length direction L is primarily related to the detection sensitivity of the detection assembly, and thus the dimensions of both the first core 32 and the second core 33 in the length direction L can be adjusted accordingly. In this way, the loss when light is introduced into the first core 32 through the incidence grating 5A is small, whereas the loss of light incident on the second core 33 through the first core 32 at the interface of the second core 33 and the reaction fluid carrier 4 (the portion of the surface of the second core 333 overlapping the reaction fluid carrier 4) due to the evanescent wave phenomenon is more remarkable, which is advantageous for obtaining a more accurate detection result finally.

Further, the reaction fluid carrier 4 may be formed in a porous configuration and fixedly disposed on the second core 33, the second core 33 being fixedly disposed on the first core 32, the reaction fluid carrier 4 effectively defining a mixing region of the detection assembly for the sample to be detected and the reaction fluid. An evanescent wave is generated when light is incident at the interface of the second core 33 and the reaction fluid carrier 4, so that the influence of the reactant generated after the reaction of the reaction component in the reaction fluid carrier 4 with the sample to be detected on the evanescent wave is utilized to qualitatively and/or quantitatively detect a specific component in the sample to be detected. In the present embodiment, when the reaction fluid corresponding to the first optical waveguide contains a buffer solution and a modified dye in addition to the reaction component, the reaction fluid corresponding to the second optical waveguide contains no reaction component and only the buffer solution and the modified dye. The reaction fluid carrier 4 is capable of holding the reaction fluid during detection with the detection assembly so that the reaction fluid can be concentrated at the second core 33 as much as possible.

It is understood that in the case where the second core 33 is provided, light propagating in the first core 32 may enter the second core 33 from the first core 32 and then exit from the second core 33 to the light detecting portion. Alternatively, the light propagating in the first core 32 may enter the second core 33 from the first core 32, and then return to the first core 32 from the second core 33 again and exit from the first core 32 to the light detecting section. In addition, the reaction fluid carrier 4 may be directly disposed on the first core 32 without disposing the second core 33.

By adopting the above-described structure, the sample to be inspected flowing through the first flow path formed by the micro flow path reacts with the reaction component in the reaction fluid entering the reaction cell via the first storage portion 113A, thereby significantly affecting the intensity and/or phase of the light propagating in the first optical waveguide path. In this way, the components corresponding to the reaction components in the sample to be examined can be qualitatively and/or quantitatively detected using the principles described previously.

The structure of the detecting assembly according to the second embodiment of the present application is described below.

(construction of a detection Assembly according to a second embodiment of the application)

The structure of the detecting assembly according to the second embodiment of the present application is substantially the same as that of the detecting assembly according to the first embodiment of the present application, and differences therebetween will be mainly described below.

In this embodiment, as shown in fig. 3A to 3C, compared with the first embodiment, the flow blocking structure 26' includes more step structures, so that more discrete height abrupt change positions can be formed, and thus, a better effect of delaying the flow of the sample to be detected from the reaction cell can be exerted.

The structure of the detecting assembly according to the third embodiment of the present application is described below.

(construction of detection Assembly according to third embodiment of the application)

The structure of the detecting assembly according to the third embodiment of the present application is substantially the same as that of the detecting assembly according to the first embodiment of the present application, and differences therebetween will be mainly described below.

In the present embodiment, as shown in fig. 4A to 4C, the first outflow branch portion 25A and the second outflow branch portion 25B are formed with a choke structure 26 "having a different configuration than the first embodiment. The flow blocking structure 26 "includes two thin sections of reduced width, a wide section being disposed between the thin sections, the width of the thin sections being narrowed relative to the width of the wide section. Therefore, the thin part with narrow width delays the outflow of the sample to be detected, and the functions of delaying the outflow of the sample to be detected from the reaction tank described in the first embodiment and the second embodiment can be exerted.

It should be understood that the above-described embodiments are merely exemplary and are not intended to limit the present application. Those skilled in the art can make various modifications and changes to the above-described embodiments without departing from the scope of the present application. Supplementary explanation is made below.

i. In the above embodiment, it can be considered that a reaction cell is formed by surrounding the cap layer 1, the runner layer 2 and the optical waveguide structure 3, and the porous reaction fluid carrier 4 accommodated in the reaction cell defines a mixing region of the reaction fluid and the sample to be inspected.

Further, in an alternative, a separate sensing layer may be provided, which may be provided between the optical waveguide structure 3 and the runner layer 2 and inside which a reactive fluid carrier 4 may be provided, the reactive fluid carrier 4 may be in direct abutment with the core of the optical waveguide structure 3.

Further, in an alternative, the reaction fluid may be stored directly in the reaction fluid carrier 4, not necessarily in the storage portions 113A, 113B of the cap layer 1, whereby the structure of the detection assembly may be further simplified.

in the above embodiment, the passage from the storage portions 113A, 113B of the cap layer 1 to the reaction cell is not necessarily limited to the specific structure described in the above embodiment, but may be changed as needed.

in the above embodiment, the optical device for capturing the light from the light source and the optical device for guiding the light propagating in the optical waveguide path to the light detection section are not limited to the gratings 5A, 5B, but may be selected as needed. In particular, the kind and arrangement position of the optical device can be selected as required with reference to the alternatives in fig. 1C to 1I. Regarding the light source, the optical system, and the light detection section combined with the aspects of the embodiments of the present application, their arrangement manner and arrangement position references may refer to the cores of the optical waveguide structures in the above-described embodiments.

In addition, in the above embodiment, when the cover layer 1 itself is made of a light-transmitting material, light from the light source may enter the light waveguide structure 3 through the cover layer 1 via the incident grating 5A, and light emitted from the exit grating 5B may be detected by the light detecting section after passing through the cover layer 1. When the cover layer 1 itself is not capable of transmitting light, through holes may be formed at the portions of the cover layer 1 where the incident grating 5A and the exit grating 5B are located.

The form of the detection assembly of the present application may be a so-called "chip". In addition, the application also provides a detection device comprising the detection component, and the chip can be inserted into the detection device in a detachable mode. The detection device may comprise a housing, a display screen, a power supply, and necessary input/output modules and ports, etc. in addition to the above-described detection assembly, and may comprise additional light sources when the detection assembly does not comprise a light source. The detection device can realize miniaturization and disposable functional characteristics. When the detection device has a disposable functional property, the outflow opening 114 in the cover layer 1 of the detection assembly may alternatively be formed as a waste liquid receptacle, not necessarily in the form of a through-hole.

The sample to be tested for by the testing assembly of the present application is not limited to blood, but may be other biological samples. The reaction fluid is typically, but not limited to, a liquid, wherein the reactive component of the reaction fluid is capable of reacting with the particular component desired to be detected in the sample to be detected, and wherein the component desired to be detected in the sample to be detected is not reactive with other components in the reaction fluid. In the case where the reaction fluid is a solid substance such as a powder, the reaction fluid (solid substance) may be directly stored in the reaction fluid carrier 4. Alternatively, the reaction fluid may include a solid substance such as a powder and a liquid, which may be stored in the reaction fluid carrier 4 and the storage portions 113A, 113B, respectively.

In an alternative, the partial structure of the detection assembly according to the above-described embodiment of the application may be manufactured using the following manufacturing method:

a film is formed as a clad layer 342 on a base layer 341 made of glass by using a silica film press, and the clad layer 342 may be formed by other means such as sputtering;

forming the first core 32, the second core 33, and the upper cladding 31 on the cladding 342 using a polymer, and forming a grating (including an incident grating 5A and an exit grating 5B) at a predetermined position of the first core 32, the shape of the grating may be as shown in fig. 2N;

Forming a silicone film on the second core 33 by spin coating, and forming a reaction fluid carrier 4 by the silicone film, removing an unnecessary portion;

the runner layer 2 is formed on the upper cladding layer 31.

Claims

1. A sensing assembly, comprising:

a cap layer (1) formed with a separate inflow hole (112) and outflow hole (114);

a flow channel layer (2) that forms a micro flow channel that communicates with the inflow hole (112) and the outflow hole (114);

an optical waveguide structure (3) forming at least two optical waveguide paths, a mixing region corresponding to each optical waveguide path being formed on a surface of an optical waveguide material of the optical waveguide structure (3), the micro flow channel being for guiding a sample to be inspected to flow from the inflow hole (112) toward the outflow hole (114) and through each mixing region such that the sample to be inspected is in contact with a reaction fluid flowing through or stored in the mixing region, light propagating through the optical waveguide path passing through the corresponding mixing region during propagation; and

2. The detection assembly of claim 1, wherein the optical system includes a first optical device and a second optical device corresponding to each of the optical waveguide paths, the first optical device for capturing light from a light source to cause light to be incident into the corresponding optical waveguide path, the second optical device for guiding light in the optical waveguide path out of the optical waveguide path,

3. The detection assembly of claim 2, wherein,

the first optical device is one or a combination of a plurality of gratings, reflectors, lenses, lens groups, lens arrays and beam splitters; and/or

4. A detection assembly according to any one of claims 1 to 3, further comprising only one light source, light from the light source being incident into all of the optical waveguide paths using the optical system.

5. The detection assembly according to claim 4, characterized in that the optical waveguide structure (3) comprises an upper cladding layer (31), a core (32) and a lower cladding layer (34), the core (32) being made of the optical waveguide material and being located between the upper cladding layer (31) and the lower cladding layer (34), the upper cladding layer (31) being in abutment with the runner layer (2).

6. The detection assembly of claim 5, further comprising a light detection portion, the light source and the light detection portion being disposed offset relative to the core (32).

7. The detection assembly of claim 6, wherein,

the light source and the light detection section are disposed on the same side of the core (32) in a thickness direction (T) of the detection assembly; or alternatively

The light source and the light detection portion are disposed on different sides of the core (32) in a thickness direction (T) of the detection assembly.

8. The detection assembly according to claim 5, further comprising a light detection portion, wherein the light source and the light detection portion are disposed so as to be aligned with opposite end faces of the core (32), and are disposed on both end sides of the core (32) of the optical waveguide structure (3), respectively.

9. The detection assembly of claim 5, further comprising a light detection section, wherein the light source comprises a self-luminescent film disposed on a surface of the core (32), and wherein the light detection section comprises a light detection film disposed on a surface of the core (32).

10. A test device comprising a test assembly according to any one of claims 1 to 9.