CN1172183C - Liquid introduction device for microanalysis chip - Google Patents
Liquid introduction device for microanalysis chip Download PDFInfo
- Publication number
- CN1172183C CN1172183C CNB011260882A CN01126088A CN1172183C CN 1172183 C CN1172183 C CN 1172183C CN B011260882 A CNB011260882 A CN B011260882A CN 01126088 A CN01126088 A CN 01126088A CN 1172183 C CN1172183 C CN 1172183C
- Authority
- CN
- China
- Prior art keywords
- liquid
- chip
- outlet
- sample
- microchip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 132
- 238000004452 microanalysis Methods 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 claims description 45
- 238000005213 imbibition Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000005484 gravity Effects 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 abstract description 32
- 239000000523 sample Substances 0.000 description 35
- 238000012544 monitoring process Methods 0.000 description 7
- 238000005251 capillar electrophoresis Methods 0.000 description 6
- 235000001014 amino acid Nutrition 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 229910052697 platinum Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 238000001499 laser induced fluorescence spectroscopy Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910021421 monocrystalline silicon Inorganic materials 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000001039 wet etching Methods 0.000 description 1
Images
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention relates to a liquid introducing device for a micro analysis chip, which comprises at least one liquid introducing opening, at least one liquid extracting opening and a flow channel, wherein the flow channel is arranged between the liquid introducing openings and the liquid extracting openings, and is communicated with at least one micro channel on a micro chip. The liquid introducing device for a micro analysis chip has the advantage that the automatic continuous liquid introduction with high efficiency is realized in a micro chip analysis system.
Description
Technical field
The present invention relates to the microchip analysis field, this domain name is more at present, title micro-total analysis system (Micro totalanalysis systems is arranged, μ TAS), title chip lab (Lab on a chip) is also arranged, or claim micro-fluidic chip (Microfluidic chips), or claim biochip (Biochip), or claim microchip analysis (Microchip analysis), below be referred to as the microchip analysis.
Background technology
Microanalysis chip usually by last 4,5 two-layer or multilayer chiops constitute down, the material of chip is monocrystalline silicon or quartzy or glass or high molecular polymer etc.; The geometric scale of chip: long is the 1-500 millimeter, and wide is the 1-500 millimeter, and thickness is the 0.1-20 millimeter; Adopt micro-processing method (be generally last slice) on chip to etch the microchannel of certain configuration, microchannel width 1-10000 micron, degree of depth 1-5000 micron; Vertically punching on chip constitutes microchannel and extraneous access way, aperture 1-50000 micron; With upper strata chip 4 and lower floor's chip 5 involutions, form the microchannel network of sealing, constitute complete microanalysis chip.The liquid storage tank of storing sample, reagent, waste liquid is arranged on the chip of upper strata, and liquid storage tank job operation in the past to be adopted as methods that figure two describes, i.e. the fixing method of column tube (internal diameter 0.1-500 millimeter, high 1-100 millimeter) formation well shape liquid storage tank on chip.At present, in the microchip analysis system that document is delivered, the common trait that is used to store the liquid storage tank of sample, reagent, waste liquid is that liquid storage tank is the single radial cut mode.The actual sample that carries out adds sample and reagent when measuring in liquid storage tank, by certain microfluidic control technology, finish on chip the compartment analysis operation to sample.When changing liquid wherein, need primary liquid in the sucking-off pond, introduce liquid with cleaning fluid or desire and clean (adding and sucking-off) liquid storage tank 1-5 time; Add the liquid that desire is introduced again.The most manual methods that adopt of the introducing method of liquid carry out (Manz-A, Becker-H, (Eds) Microsystem Technology inChemistry and Life Sciences, Springer, Berlin, 1999), minority adopts automated process to finish (Van denBerg-A, Olthuisi-W, Bergveld-P, (Eds.), Micro Total Analysis Systems 2000, KluverAcademic Publishers, The Netherlands, 2000,391).In the microchip compartment analysis, the introducing of relevant sample operation is carried out more.Promptly when analytic system was measured different samples, need employing craft or automated process were repeatedly changed the sample (promptly varying operation) in the sample liquid storage tank.With the more employing electroosmotic pump of bibliographical information is that the microfluidic analysis system of power is an example, and its concrete operations comprise usually: cut off operating voltage; Shift out electrode in the sample cell, cleaning electrode; Central Plains, sucking-off pond sample solution; Clean (adding and sucking-off) sample cell 1-5 time with cleaning fluid or fresh sample solution; Add fresh sample solution; Electrode moves in the sample cell; Open operating voltage, system recovery work.Though this mode of varying that adopts is operated simply at present, its shortcoming is that complex steps is time-consuming, and efficient is not high; Adopt discontinuous formula sample introducing method need interrupt the analysis operation of sample, reduced the analysis speed of system in the stage of varying; System also is difficult to carry out a fairly large number of sample determination and online process monitoring.Also useful automated process (Van den Berg-A, Olthuisi-W, Bergveld-P, (Eds.), Micro Total AnalysisSystems 2000, Kluver Academic Publishers, The Netherlands, 2000,391) replace manual method to realize the report that varies continuously, though this system can realize sample and introduce automation of operation, its process is imitated manual step substantially, and it is still not high to vary efficient.
At present, a kind of solution thinking of relevant this problem is to adopt disposable sample introducing method, avoids the replacing operation of sample.Its concrete grammar comprises: the method that adopts disposable use chip in microchip analysis system; Method (the Agilent Bioanalyzer 2100 of a plurality of sample cells is processed in employing on chip, http://www.chem.agilent.com), or on chip processing a plurality of analytic units method (Shi-Y, Simpson-PC et al., Anal.Chem.71,1999,535) measure the preceding a plurality of samples of disposable introducing, on chip, realize mensuration various sample.But this method is varied the restriction that quantity is subjected to die size, varies the program underaction, and speed is slow, also is difficult to carry out online process monitoring.And under current chip cost condition with higher, the chip of replication ability adopts disposable method to still having repeatedly, is unfavorable for the popularization of method.Continue to use the problem of varying that then still exists to change all samples on the chip as need.
Summary of the invention
The purpose of this invention is to provide a kind of liquid that is used for microanalysis chip and introduce interface arrangement, replace single radial cut formula liquid storage tank in the past, realize that high efficiency continuous liquid is introduced on the microchip.Having liquid on this apparatus function simultaneously leads in/out and two functions of liquid storage continuously.This device both can be used for the storage and the replacing of sample on the chip, also can be used for the storage and the replacing of other solution in the microchip analysis system or liquid.
What the present invention relates to is that flow type liquid on the microanalysis chip is introduced interface arrangement, according to the present invention, it is characterized in that this device is made of flow channel three parts that communicate with at least one microchannel on the microchip between at least one liquid intake, at least one liquid outlet, at least one liquid intake and the liquid outlet.
According to the present invention, the flow channel between described liquid intake, liquid outlet, liquid intake and the liquid outlet, the cross-sectional area of three's passage is between 100 square microns-100 square millimeter.Adopt bigger cross-sectional area, help reducing liquid and introduce the interference of flowing inner analysis process in microchannel on the microchip.Adopt less cross-sectional area to help reducing liquid introducing amount, save the liquid that is introduced into.
According to the present invention, liquid intake and the liquid outlet flow channel configuration between the two can have multiple choices, and that easily realizes has: linear path, and liquid intake and liquid outlet lay respectively at the two ends of linear flow channel; " U " type passage, liquid intake and liquid outlet lay respectively at the two ends of " U " shape flow channel; " Z " type passage, liquid intake and liquid outlet lay respectively at the two ends of " Z " shape flow channel; Bore is than the thimble tube passage of major path tape footpath smaller channels.
Beneficially, the setting of linear flow channel on chip between liquid intake and the liquid outlet adopts the vertical-type passage along gravity direction, or the horizontal type passage of passage on same surface level.
According to the present invention, described device can have two or more liquid intakes to be connected with flow channel, introduces when realizing multiple liquid.
The introducing of liquid in apparatus of the present invention, draw operation and can cause the contacted outlet liquid level of liquid and atmosphere in the liquid storage tank that the variation of height takes place usually, the variation that produces liquid level difference on the chip then might be disturbed normal compartment analysis process on the chip.According to another characteristics of the present invention, it is characterized in that utilizing the overflow phenomena of trickle at the liquid outlet, by regulating the liquid outlet to predetermined horizontal level, and in analytic process, keep its horizontal level constant, reach in the regulating device liquid horizontal level of liquid level that contacts with atmosphere, and remain on the stable purpose of this liquid level horizontal level in the analytic process.Its principle is to utilize the gravity and the flowability of liquid self, when introducing liquid by flow type liquid storage tank inlet, excess liquid is overflowed by its overflow type outlet and is flowed away in the pond, outlet liquid level in the liquid storage tank remain with export identical surface level on, the horizontal level of overflow type outlet promptly is the liquid level position of device middle outlet.In analytic process,, just can guarantee the constant of its outlet liquid level position as long as guarantee that the horizontal level of flow type liquid storage tank overflow type outlet is constant.
Beneficially, the Way out of aforesaid liquid outlet should be along gravity direction vertically upward.
Another characteristics according to the present invention, what relate to is that relevant overflow type outlet is made, and it is characterized in that installing around the liquid outlet that installs imbibition water conservancy diversion cover.Purpose is to utilize the stronger imbibition ability of imbibition material, Liquid Absorption and the guiding that the liquid storage tank outlet is overflowed flows into waste fluid container fast, to avoid being changed by the rate of outflow in the liquid storage tank because of liquid the fluctuation of the flow type liquid storage tank overflow type outlet liquid level position that is caused.Simpler method is to adopt the filter paper sleeve to make aforesaid imbibition water conservancy diversion cover.
According to another characteristics of the present invention, what relate to is the buffer-type level stability device that other single radial cut liquid storage tanks on the microanalysis chip of apparatus of the present invention are installed.Purpose is that the liquid level of liquid only produces less relatively variation in the liquid storage tank in the compartment analysis process of chip, and unlikely have remarkable influence to the compartment analysis process, thus make the microanalysis chip liquid introduction device can be in longer a period of time continuous firing.The feature of this level stability device is to use the liquid storage tank with bigger horizontal sectional area, makes in chip compartment analysis process, and within a certain period of time, the variation of liquid volume only produces very little liquid level change relatively in the liquid storage tank.More advantageously, the horizontal sectional area of aforesaid single radial cut liquid storage tank should be greater than 50 square millimeters.
Advantage of the present invention is that the high efficiency continuous liquid that can realize robotization is introduced; The analysis operation that varies operation and sample can carry out simultaneously, does not disturb mutually, and the influence of operation is not varied by system's other analytical performances (as analysis speed, separating property etc.); The amount of varying is little, saves sample; The speed of varying is fast, can carry out the mensuration of high-throughout different samples; Be suitable for carrying out the fast measuring of many samples, be particularly suited for carrying out high-resolution online process monitoring and can carry out high-resolution online process monitoring.
Description of drawings
Fig. 1. be that 1 capillary electrophoresis analysis microchip in accordance with a preferred embodiment of the present invention is installed;
Fig. 2. be the side sectional view of the single radial cut formula liquid storage tank that uses on Fig. 1 microchip;
Fig. 3. be Fig. 1. in according to the side sectional view of liquid introduction device of the present invention;
Fig. 4. be 2 side sectional view according to still another embodiment of the invention;
Fig. 5. be the vertical view of embodiment 2;
Fig. 6. be 3 side sectional view according to still a further embodiment;
Fig. 7. be the vertical view of embodiment 3;
Fig. 8. be 4 side sectional view according to still a further embodiment;
Fig. 9. be the vertical view of embodiment 4;
Figure 10. be 5 side sectional view according to still a further embodiment;
Figure 11. show and investigate the outcome record figure that embodiment 1 device is carried performance outside;
Figure 12. show microchip analysis system that embodiment 1 device is installed carries out express-analysis continuously to the different aminoacids sample record diagram;
Figure 13. show that the microchip analysis system that embodiment 1 device is installed is applied to the record diagram of on-line monitoring fluorescein isothiocynate (FITC) mark different aminoacids course of reaction.
Embodiment
With reference to accompanying drawing, below will describe according to a preferred embodiment of the present invention 1 in detail.
Fig. 1. be the capillary electrophoresis analysis microchip that is equipped with according to a kind of liquid introduction device of the present invention.Chip is made up of last 4, following 5 liang of glass sheet, and glass sheet length, width, thickness are respectively 60 millimeters, 30 millimeters, 1.7 millimeters.Adopt classical photomask and wet etching technique last 4 processing cruciform microchannel.The microchannel width is 100 microns, and the degree of depth is 23 microns.Split tunnel 7 length are 38 millimeters, and sample intake passage 6 length are 15 millimeters, both in distance split tunnel 7 ends 5 millimeters locate to intersect.Respectively in the boring of the end of cruciform microchannel, form the duct of the connection microchannel 6 of corresponding liquid storage tank 8,9,10 of difference and liquid introduction device of the present invention 11 with 1.7 millimeters drill bits.Simultaneously,, treat 4, behind following 5 two involutions, can form the inlet 1 of up/down perforation and export 2 vertical channel 3 with identical bit bore in the position of following sheet 5 corresponding apparatus of the present invention 11.Adopt the method for classical high temperature bonding to realize last 4, following 5 two permanent involution.
Fig. 2 is the side sectional view that adopts the single radial cut formula liquid storage tank of classical way making.Intercepting the cylindrical plastic pipe 17 of 10 millimeters high of 1.5 milliliters of centrifuge tube ends, is that the center of circle sticks with glue agent 18 and is fixed in slices 4 with boring, forms the inner liquid storage tank 8,9,10 that certain liq 19 is housed.
Fig. 3 is the side sectional view according to liquid introduction device 11 of the present invention.The method for making of apparatus of the present invention 11 outlets 2 is: get one 6 millimeters high, 2.3 millimeters of internal diameters suitable for reading, the conical tube 22 that the end opening internal diameter is 1.7 millimeters, be that the center of circle sticks with glue agent 18 and is fixed on slices 4 with boring, the conical tube outer ring is with filter paper sleeve 21 parcels, and filter paper sleeve 21 upper edges exceed 2 millimeters of conical tube 22 upper edges.With 30 millimeters long, the semicircle plastics diversion trench 12 of 6 mm wides is fixed in around conical tube 22 and the filter paper sleeve 21 with tackifier 18, place 40 millimeters long in the diversion trench 12, the water conservancy diversion filter paper bar 13 of 4 mm wides, water conservancy diversion filter paper bar 13 1 ends contact with filter paper sleeve 21, and an end places the outlet of diversion trench 12.The method for making of apparatus of the present invention 11 inlets 1 is: get one 6 millimeters high, and 1.5 millimeters of external diameters suitable for reading, the conical tube 23 that the end opening external diameter is 2.5 millimeters is with 1 millimeters deep in the conical tube 23 insertion borings suitable for reading.Get one 30 millimeters long, 0.5 millimeter of internal diameter, the elastoplast pipe 15 that external diameter is 1.5 millimeters inserts in the end opening of conical tube 23.Get 10 millimeters long, the platinum filament 24 of 0.3 mm outer diameter passes conical tube 23 tube walls and inserts in the boring, and its tip is inserted to the little sample intake passage of chip 6 porch, and the other end of platinum filament 24 links to each other with Capillary Electrophoresis power lead 16.With tackifier 18 said apparatus is fixed in sheet 5 under the chip respectively.Elastoplast pipe 15 that chip is outer and power lead 16 parts are drawn by sleeve pipe 14 parcels.
Apparatus of the present invention 11 are by 0.35 internal diameter, and the teflon catheter of 20 centimeter length links to each other with the peristaltic pump that varies power is provided.Apparatus of the present invention 11 adopt power supplys to suspend or install the method for 11 ground connection, avoid in the capillary electrophoresis analysis work sample introduction high voltage to varying the influence of power system.
In microchip capillary electrophoretic separation analytic process, can adopt the multiple method of varying.Wherein comparatively commonly used is: vary in certain period of detachment process, carry out simultaneously with capillary electrophoresis separation, arrhea in other separation and sample introduction period apparatus of the present invention 11; The another kind method of varying is that bubble varies method at interval, and sample is introduced into apparatus of the present invention 11 under two sections air are carried secretly, and the system that makes can adopt less volume of sample, plays the saving sample, accelerates the effect of the speed of varying; Another method of varying is the mode of varying continuously, when the compartment analysis process is carried out, varies the operation nothing and intermittently carries out continuously.
Adopt above device, reach laser induced fluorescence detector, separated the amino acid of fluorescein isothiocynate (FITC) mark with capillary electrophoresis.Figure 11, Figure 12 and Figure 13 are the record diagrams of separating resulting.Figure 11 show circulation in the present embodiment vary the pond to the amount of carrying outside of variable concentrations sample less than 1.5%., wherein sample 1 differs one times with sample 2 concentration, and the superperformance of this liquid introduction device aspect carrying outside is described.Figure 12 is that microchip analysis system has shown that at short notice to the measurement result of various sample this liquid introduction device has the ability of various sample being carried out high throughput analysis in the present embodiment.Figure 13 carries out on-line monitoring fluorescein isothiocynate (FITC) mark different aminoacids course of reaction record diagram for microchip analysis system in the present embodiment, shows that this liquid introduction device has the ability long-time, the online process monitoring of high-resolution of carrying out.
With reference to attached Figure 4 and 5, below will describe according to still another embodiment of the invention 2.
Fig. 4 is the side sectional view according to embodiments of the invention 2.Fig. 5 is the vertical view of embodiment 2.Wherein, liquid intake 1 and liquid outlet 2 flow channel 3 between the two is configured as linear path, and passage is the horizontal type passage on same surface level, and in the middle of the passage 3 and microchannels 6 on the microchip communicate.Simultaneously, this device adopts two liquid intakes 1, can introduce two kinds of different liquid 20 and 25 simultaneously.Thick arrow represents to be introduced into the flow direction of liquid.
With reference to accompanying drawing 6 and 7, below will describe according to still a further embodiment 3.
Fig. 6 is the side sectional view according to embodiments of the invention 3.Fig. 7 is the vertical view of embodiment 3.Wherein, liquid intake 1 and liquid outlet 2 flow channel 3 between the two is vertical " U " type channel configurations.Liquid intake 1 and liquid outlet 2 lay respectively at the two ends of " U " shape flow channel 3.Passage 3 microchannels 6 middle and on the microchip communicate.Thick arrow represents to be introduced into the flow direction of liquid.
With reference to accompanying drawing 8 and 9, below will describe according to still a further embodiment 4.
Fig. 8 is the side sectional view according to embodiments of the invention 4.Fig. 9 is the vertical view of embodiment 4.Wherein, liquid intake 1 and liquid outlet 2 flow channel 3 between the two is " Z " shape channel configurations.Liquid intake 1 and liquid outlet 2 lay respectively at the two ends of " Z " shape flow channel 3.Passage 3 microchannels 6 middle and on the microchip communicate.Thick arrow represents to be introduced into the flow direction of liquid.
With reference to accompanying drawing 10, below will describe according to still a further embodiment 5.
Figure 10 is the side sectional view of embodiment 5.Wherein, an external diameter inserts in the bigger passage 3 of microchip top external diameter than ductule 26, forms the thimble tube channel architecture.Passage 3 bottoms communicate with microchannel 6 on the microchip.Thick arrow represents to be introduced into the flow direction of liquid.
Claims (3)
1, a kind of device that is used for the introducing of microanalysis chip liquid, this device is by at least one liquid intake, at least one liquid outlet, flow channel three parts that communicate with at least one microchannel on the microchip between at least one liquid intake and the liquid outlet constitute, it is characterized in that, described liquid outlet is the outlet of overflow shape, direction is along gravity direction vertically upward, by regulating the liquid outlet to predetermined horizontal level, and in analytic process, keep its horizontal level constant, reach in the regulating device liquid horizontal level of liquid level that contacts with atmosphere, and remain in the analytic process this liquid level horizontal level and stablize.
2, the microanalysis chip liquid introduction device that is used for according to claim 1 is characterized in that, in the described device liquid outlet around imbibition water conservancy diversion cover is installed.
3, the microanalysis chip liquid introduction device that is used for according to claim 2 is characterized in that, described imbibition water conservancy diversion cover adopts the filter paper sleeve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011260882A CN1172183C (en) | 2001-08-21 | 2001-08-21 | Liquid introduction device for microanalysis chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011260882A CN1172183C (en) | 2001-08-21 | 2001-08-21 | Liquid introduction device for microanalysis chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1402010A CN1402010A (en) | 2003-03-12 |
| CN1172183C true CN1172183C (en) | 2004-10-20 |
Family
ID=4666159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011260882A Expired - Fee Related CN1172183C (en) | 2001-08-21 | 2001-08-21 | Liquid introduction device for microanalysis chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1172183C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1941941A1 (en) * | 2005-10-24 | 2008-07-09 | Yokogawa Electric Corporation | Microchip device |
| CN100392316C (en) * | 2006-03-27 | 2008-06-04 | 博奥生物有限公司 | Flow structure of controlling liquid continuously flowing in micro-pipeline |
| CN103558126B (en) * | 2013-11-07 | 2015-09-23 | 深圳中科强华科技有限公司 | The device and method of particle in Sequencing and analysis liquid |
-
2001
- 2001-08-21 CN CNB011260882A patent/CN1172183C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1402010A (en) | 2003-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7695629B2 (en) | Microfluidic separator | |
| US7259020B2 (en) | Microfabricated structures for facilitating fluid introduction into microfluidic devices | |
| EP1487581B1 (en) | Microfluidic channel network device | |
| US6488896B2 (en) | Microfluidic analysis cartridge | |
| EP3993905B1 (en) | Microfluidic device and method for processing and aliquoting a sample liquid | |
| CN1172183C (en) | Liquid introduction device for microanalysis chip | |
| US20060204403A1 (en) | Micro-fluidic fluid separation device and method | |
| CN109746063A (en) | Microlayer model detection system | |
| Cai et al. | A gravity driven micro flow injection wetting film extraction system on a polycarbonate chip | |
| CN1818662A (en) | Sequential injection analyzer based on capillaries and utilization thereof | |
| Chen et al. | A Multiphase laminar flow diffusion chip with ion selective electrode detection | |
| CN100389321C (en) | Liquid level stabilizing device in micro-analysis chip liquid pool and method of use thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20041020 Termination date: 20110821 |