CN117210318A - Enterobacter detection kit - Google Patents
Enterobacter detection kit Download PDFInfo
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- CN117210318A CN117210318A CN202311010237.3A CN202311010237A CN117210318A CN 117210318 A CN117210318 A CN 117210318A CN 202311010237 A CN202311010237 A CN 202311010237A CN 117210318 A CN117210318 A CN 117210318A
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- Prior art keywords
- mixing chamber
- box body
- enterobacteria
- detection kit
- detection
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- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 241000588914 Enterobacter Species 0.000 title description 2
- 238000002156 mixing Methods 0.000 claims abstract description 30
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 23
- 241000305071 Enterobacterales Species 0.000 claims abstract description 21
- 238000010438 heat treatment Methods 0.000 claims abstract description 14
- 241000588724 Escherichia coli Species 0.000 claims description 16
- 238000005516 engineering process Methods 0.000 claims description 8
- 238000007397 LAMP assay Methods 0.000 claims description 5
- 101150038062 fliC gene Proteins 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 239000002313 adhesive film Substances 0.000 claims description 3
- 239000000523 sample Substances 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 13
- 230000001717 pathogenic effect Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 238000011161 development Methods 0.000 description 5
- 230000000369 enteropathogenic effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000003753 real-time PCR Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 101100281124 Aquifex aeolicus (strain VF5) flaA gene Proteins 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 208000005577 Gastroenteritis Diseases 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 208000012873 acute gastroenteritis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application belongs to the technical field of biological detection, in particular to an enterobacteria detection kit, which comprises a kit body and a mixing chamber; the top of the box body is provided with a box cover, the front surface of the box cover is provided with an observation window, one side of the box body is provided with a battery groove, and the battery groove is provided with a groove cover; the utility model discloses a mixing chamber, including box body, mixing chamber, micro motor, heating seat, reagent reaction package, the top of heating seat is provided with sealed lid, the bottom of mixing chamber is provided with micro motor, micro motor's output extends to the interior chamber of mixing chamber and is provided with the axis of rotation, the surface of axis of rotation is provided with the puddler, one side of mixing chamber is provided with the micropump, the output of micropump is provided with the shunt tubes, the inner chamber opposite side of box body is provided with the heating seat, the top of heating seat is provided with reagent reaction package, and its is rational in infrastructure, in-process that uses, the condition of convenient control detection, the operation degree of difficulty is low, and application scope is wide, and need not with the help of other instruments, and detection efficiency is high.
Description
Technical Field
The application relates to the technical field of biological detection, in particular to an enterobacteria detection kit.
Background
Coli has pathogenic and non-pathogenic components, where pathogenic escherichia coli can cause food poisoning. Pathogenic E.coli is classified into invasive and toxic types. Diarrhea caused by the former is similar to dysentery caused by bacillus dysenteriae, and is generally called acute dysentery type; diarrhea caused by the latter is of the gastroenteritis type, commonly referred to as acute gastroenteritis type.
The traditional detection method of pathogenic escherichia coli needs fermentation or enrichment, colony purification and separation, physiological and biochemical identification, serological identification and the like, is time-consuming and labor-consuming, has complex steps, and cannot meet the rapid, simple and high-specificity identification requirements of the pathogenic escherichia coli. The newly developed method for detecting pathogenic escherichia coli by using the molecular biology technology can greatly improve the detection working efficiency. However, the existing molecular biological method for detecting pathogenic escherichia coli often needs more than ten reagents, and the reagents are expensive, small in dosage and need to be preserved or used in a low-temperature sterile mode, so that interference on detection results is reduced. For example, CN103361414a discloses a LAMP detection method of enteropathogenic escherichia coli, which has the advantages of good sensitivity, rapidness, reliability, simple operation and simple identification. CN102605068A discloses a method for detecting enteropathogenic escherichia coli by fluorescence PCR, which can also rapidly detect enteropathogenic escherichia coli. The kit needs more than ten reagents such as primers, enzyme, DNA probe templates, buffer solution, color development solution, sterile ultrapure water and the like, and the existing kit only stores reagent test tubes, and uses equipment such as a pipetting gun, an ultra-clean bench and the like, so that the kit is very inconvenient and complex to operate, and the detection speed is slowed down.
The traditional escherichia coli detection method has the defects of long detection period, complex procedure, multiple required reagents and the like, and can not meet the modern detection requirements. The pathogenic nucleic acid detection techniques represented by the Polymerase Chain Reaction (PCR) technique have some problems in practical application, such as the necessity of special instruments for the ordinary Polymerase Chain Reaction (PCR) technique, and the disadvantages of easy cross contamination and cumbersome operation procedures. While fluorescence real-time quantitative polymerase chain reaction (real time PCR) technology better solves the problem of cross contamination and simplifies the operation process, it requires more complex quantitative measuring instruments and is therefore not suitable for rapid detection on site. And the cost of the fluorescent probe in the real-time quantitative polymerase chain reaction PCR technology is high, and the difficulty of popularization and application is increased. The immunological detection technology is quick, simple and low in cost, but requires a monoclonal antibody with high quality and high stability, otherwise, the monoclonal antibody is not accurate enough, and can only be an auxiliary detection means at present. So the latest results of timely applying biotechnology development have important significance for meeting the continuous improvement of the detection requirements of pathogenic microorganisms.
In the prior art, the application name is CN201010181095.3, the application number is an escherichia coli detection kit and a use method thereof, and in the recorded technical scheme, the detection time is short, the detection result is convenient to observe, the detection effect is more comprehensive, the specificity is high, and the omission factor is low; however, in the practical use process, the kit still has some defects, has higher detection conditions, is inconvenient to control the detection conditions, needs special medical staff to operate, has a small application range, and provides a novel enterobacteria detection kit for the purpose.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.
The present application has been made in view of the problems occurring in the prior art.
Therefore, the application aims to provide the enterobacteria detection kit, which can realize the conditions of convenient control detection in the use process, has low operation difficulty and wide application range, does not need other tools, and has high detection efficiency.
In order to solve the technical problems, according to one aspect of the present application, the following technical solutions are provided:
an enterobacteria detection kit comprising a box body and a mixing chamber;
wherein,
the top of the box body is provided with a box cover, the front surface of the box cover is provided with an observation window, one side of the box body is provided with a battery groove, and the battery groove is provided with a groove cover;
the utility model discloses a reagent reaction package, including box body, micro-motor, mixing chamber, stirring rod, micro-pump, heating seat, reagent reaction package, shunt tubes, mixing chamber sets up the inner chamber at the box body, the top of mixing chamber is provided with sealed lid, the bottom of mixing chamber is provided with micro-motor, micro-motor's output extends to the mixing chamber intracavity and is provided with the axis of rotation, the surface of axis of rotation is provided with the puddler, one side of mixing chamber is provided with the micro-pump, the output of micro-pump is provided with the shunt tubes, the inner chamber opposite side of box body is provided with the heating seat, the top of heating seat is provided with reagent reaction package, and reagent reaction package is connected with the other end of shunt tubes.
As a preferred embodiment of the kit for detecting enterobacteria of the present application, there is provided: the front surface of lid is provided with the control box, the front surface of control box is provided with display screen and temperature sensor, and temperature sensor's probe extends to the box body inner chamber, temperature sensor and display electric connection.
As a preferred embodiment of the kit for detecting enterobacteria of the present application, there is provided: the magnetic attraction blocks are arranged on two sides of the top of the box body, and the matched magnetic attraction sheets are arranged on two sides of the bottom of the box cover.
As a preferred embodiment of the kit for detecting enterobacteria of the present application, there is provided: the middle of the shunt tube is provided with an adhesive film layer, and the shunt tube is also provided with a one-way valve.
As a preferred embodiment of the kit for detecting enterobacteria of the present application, there is provided: the reagent reaction package comprises two pairs of primers designed based on loop-mediated isothermal amplification technology by taking fliC gene of escherichia coli as target gene: inner primer FIP/BIP and outer primer F3/B3; and (5) developing a color solution.
As a preferred embodiment of the kit for detecting enterobacteria of the present application, there is provided: an insulating layer is arranged on the inner side of the box body.
As a preferred embodiment of the kit for detecting enterobacteria of the present application, there is provided: an independent power supply is arranged in the inner cavity of the control box, and the independent power supply is a button cell.
As a preferred embodiment of the kit for detecting enterobacteria of the present application, there is provided: and a loudspeaker is arranged on the display screen externally.
Compared with the prior art, the application has the beneficial effects that: through being provided with the mixing chamber in the inside of box body, can treating the solution that detects and carry out intensive mixing, improve the accuracy that detects, through being provided with the reagent reaction package in the inside of box body, including two pairs of primers that the fliC gene with escherichia coli is target gene, based on loop-mediated isothermal amplification technical design in the reagent reaction package: inner primer FIP/BIP and outer primer F3/B3; the color development liquid has low operation difficulty, wide application range, no need of other tools and high detection efficiency; in addition, through being provided with temperature sensor and carrying out real-time detection to the temperature of heating seat, the convenient control reaction condition, further improvement detects the accuracy.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following detailed description of the embodiments of the present application will be given with reference to the accompanying drawings, which are to be understood as merely some embodiments of the present application, and from which other drawings can be obtained by those skilled in the art without inventive faculty. Wherein:
FIG. 1 is a schematic diagram of the structure of the present application;
FIG. 2 is a schematic diagram of the internal structure of the present application;
FIG. 3 is a schematic view of the mixing chamber structure of the present application;
fig. 4 is a schematic view of a shunt tube according to the present application.
In the figure; 100 box bodies, 110 box covers, 120 observation windows, 130 battery grooves, 140 groove covers, 150 magnetic attraction blocks, 160 magnetic attraction sheets, 200 mixing chambers, 210 sealing covers, 220 micro motors, 230 rotating shafts, 240 stirring rods, 250 micro pumps, 260 shunt pipes, 261 sticky film layers, 262 check valves, 270 reagent reaction bags, 280 heating seats, 300 control boxes, 310 display units and 320 temperature sensors.
Detailed Description
In order that the above objects, features and advantages of the application will be readily understood, a more particular description of the application will be rendered by reference to the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, but the present application may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present application is not limited to the specific embodiments disclosed below.
Next, the present application will be described in detail with reference to the drawings, wherein the sectional view of the device structure is not partially enlarged to general scale for the convenience of description, and the drawings are only examples, which should not limit the scope of the present application. In addition, the three-dimensional dimensions of length, width and depth should be included in actual fabrication.
For the purpose of making the objects, technical solutions and advantages of the present application more apparent, embodiments of the present application will be described in further detail below with reference to the accompanying drawings.
The application provides the following technical scheme: the enterobacteria detection kit is convenient to control detection conditions in the use process, has low operation difficulty and wide application range, does not need other tools, has high detection efficiency, and is shown in fig. 1-4, and comprises a kit body 100, a mixing chamber 200 and a control box 300;
referring to fig. 1 to 4 again, the top of the case body 100 is connected with a case cover 110 through a hinge, the front surface of the case cover 110 is screwed with an observation window 120, one side of the case body 100 is provided with a battery groove 130, the battery groove 130 is screwed with a groove cover 140, two sides of the top of the case body 100 are adhered with magnetic attraction blocks 150, and two sides of the bottom of the case cover 110 are adhered with matched magnetic attraction sheets 160;
referring to fig. 1-4 again, the mixing chamber 200 is screwed in the inner cavity of the case 100, the top of the mixing chamber 200 is screwed with the sealing cover 210, the bottom of the mixing chamber 200 is screwed with the micro motor 220, the output end of the micro motor 220 extends to the inner cavity of the mixing chamber 200 and is screwed with the rotating shaft 230, the surface of the rotating shaft 230 is screwed with the stirring rod 240, one side of the mixing chamber 200 is screwed with the micro pump 250, the output end of the micro pump 250 is inserted with the shunt tube 260, the other side of the inner cavity of the case 100 is screwed with the heating seat 280, the top of the heating seat 280 is adhered with the reagent reaction package 270, the reagent reaction package 270 is connected with the other end of the shunt tube 260, and the reagent reaction package 270 comprises two pairs of primers designed based on the loop-mediated isothermal amplification technology by using fliC genes of escherichia coli as target genes: inner primer FIP/BIP and outer primer F3/B3; the middle of the color development liquid and the shunt tube 260 is adhered with an adhesive film layer 261, and the shunt tube 260 is also screwed with a one-way valve 262;
referring to fig. 1 to 4 again, the front surface of the box cover 110 is screwed with a control box 300, the front surface of the control box 300 is screwed with a display screen 310 and a temperature sensor 320, a probe of the temperature sensor 320 extends to the inner cavity of the box body 100, the temperature sensor 320 is electrically connected with a display, the inner cavity of the control box 300 is screwed with an independent power supply, and the independent power supply is a button cell; the display screen 310 has a speaker externally mounted thereon.
Working principle: in the process of using the kit, the solution to be detected can be fully mixed by arranging the mixing chamber 200 in the box body 100, so that the detection accuracy is improved, and the kit is provided with the reagent reaction package 270 in the box body 100, wherein the reagent reaction package 270 comprises two pairs of primers which are designed based on the loop-mediated isothermal amplification technology by taking fliC genes of escherichia coli as target genes: inner primer FIP/BIP and outer primer F3/B3; the color development liquid has low operation difficulty, wide application range, no need of other tools and high detection efficiency; in addition, through being provided with temperature sensor 320 and carrying out real-time detection to the temperature of heating seat 280, the convenient control reaction condition further improves the accuracy of detection.
Although the application has been described hereinabove with reference to embodiments, various modifications thereof may be made and equivalents may be substituted for elements thereof without departing from the scope of the application. In particular, the features of the disclosed embodiments may be combined with each other in any manner as long as there is no structural conflict, and the exhaustive description of these combinations is not given in this specification merely for the sake of omitting the descriptions and saving resources. Therefore, it is intended that the application not be limited to the particular embodiment disclosed, but that the application will include all embodiments falling within the scope of the appended claims.
Claims (8)
1. The enterobacteria detection kit is characterized in that: comprises a box body (100) and a mixing chamber (200);
wherein,
the top of the box body (100) is provided with a box cover (110), the front surface of the box cover (110) is provided with an observation window (120), one side of the box body (100) is provided with a battery groove (130), and the battery groove (130) is provided with a groove cover (140);
mixing chamber (200) set up the inner chamber at box body (100), the top of mixing chamber (200) is provided with sealed lid (210), the bottom of mixing chamber (200) is provided with micromotor (220), the output of micromotor (220) extends to mixing chamber (200) inner chamber and is provided with axis of rotation (230), the surface of axis of rotation (230) is provided with puddler (240), one side of mixing chamber (200) is provided with micropump (250), the output of micropump (250) is provided with shunt tubes (260), the inner chamber opposite side of box body (100) is provided with heating seat (280), the top of heating seat (280) is provided with reagent reaction package (270), and reagent reaction package (270) are connected with the other end of shunt tubes (260).
2. The enterobacteria detection kit according to claim 1, wherein: the front surface of lid (110) is provided with control box (300), the front surface of control box (300) is provided with display screen (310) and temperature sensor (320), and the probe of temperature sensor (320) extends to box body (100) inner chamber, temperature sensor (320) and display electric connection.
3. The enterobacteria detection kit according to claim 1, wherein: magnetic attraction blocks (150) are arranged on two sides of the top of the box body (100), and matched magnetic attraction sheets (160) are arranged on two sides of the bottom of the box cover (110).
4. The enterobacteria detection kit according to claim 1, wherein: an adhesive film layer (261) is arranged in the middle of the shunt tube (260), and a one-way valve (262) is further arranged on the shunt tube (260).
5. The enterobacteria detection kit according to claim 1, wherein: the reagent reaction package (270) comprises two pairs of primers which are designed based on loop-mediated isothermal amplification technology by taking fliC gene of escherichia coli as target gene: inner primer FIP/BIP and outer primer F3/B3; and (5) developing a color solution.
6. The enterobacteria detection kit according to claim 1, wherein: an insulation layer is arranged on the inner side of the box body (100).
7. The enterobacteria detection kit according to claim 2, wherein: an independent power supply is arranged in the inner cavity of the control box (300), and the independent power supply is a button battery.
8. The enterobacteria detection kit according to claim 2, wherein: and a loudspeaker is externally arranged on the display screen (310).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311010237.3A CN117210318A (en) | 2023-08-11 | 2023-08-11 | Enterobacter detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311010237.3A CN117210318A (en) | 2023-08-11 | 2023-08-11 | Enterobacter detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117210318A true CN117210318A (en) | 2023-12-12 |
Family
ID=89045103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311010237.3A Withdrawn CN117210318A (en) | 2023-08-11 | 2023-08-11 | Enterobacter detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117210318A (en) |
-
2023
- 2023-08-11 CN CN202311010237.3A patent/CN117210318A/en not_active Withdrawn
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20231212 |
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| WW01 | Invention patent application withdrawn after publication |