CN117159212A - Blood purification rabbit model and construction method and application thereof - Google Patents
Blood purification rabbit model and construction method and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of animal model construction, in particular to a blood purification rabbit model, and a construction method and application thereof. The invention relates to a construction method of a blood purification rabbit model, which comprises the following steps: a closed needle-prick-prevention venous indwelling needle is adopted for carrying out rabbit vein or artery puncture catheterization, and a blood purification rabbit model is established; the path of the tube is an internal carotid artery puncture tube, a femoral artery puncture tube, an internal jawbone vein puncture tube or an ear central artery puncture tube. The invention provides a new Zealand rabbit blood purification model established by utilizing the closed type puncture-injury-preventing vein or artery indwelling needle to perform internal carotid artery puncture catheterization, femoral artery puncture catheterization or internal jawbone vein puncture catheterization or central auricular artery catheterization for the first time, and provides guidance and reference for later blood purification treatment.
Description
Technical Field
The invention relates to the technical field of animal model construction, in particular to a blood purification rabbit model, and a construction method and application thereof.
Background
The blood purification treatment is widely applied to rescuing toxicosis, substitution treatment of acute and chronic liver function or renal failure, autoimmune diseases, preparation before organ transplantation and the like because of being capable of directly and rapidly removing pathogenic components in blood. Animal models are an indispensable experimental means for studying blood purification treatments. The selection of experimental animals and the selection of a tube placing mode are the core of establishing a blood purification animal model. According to different experimental purposes, large animals such as pigs and sheep and small animals such as mice are selected. Large animals are expensive and individual differences between hybrid animals are large. The price of the pure breed small animals is low, the individual difference is small, but the small animals have small weight, small blood volume and large operation difficulty, and the cardiovascular system is fragile, so the success rate of the operation is low. In recent years, blood purification experimental studies have been reported using New Zealand rabbits. The traditional blood purification treatment animal model is mainly provided with a carotid artery incision catheter or femoral artery incision catheter, and the two catheter methods have the common defects that the operation side artery cannot be reserved after operation and risks of wound infection, tissue ischemia and animal death are caused.
In 2007, in the study of experimental allergic neuritis by whole blood perfusion immunoadsorption treatment, related scholars abandon the prior animals such as pigs, dogs and the like from the aspects of economy, feasibility and safety, and use New Zealand rabbits as experimental animals, and after operation, the blood volume of the New Zealand rabbits is proved to be large, the operation tolerance is good, and the blood index detection is convenient; the physiological and biochemical data are rich, and can be compared and referenced; the method is relatively low in cost, mild in sex, and easy to manage and feed. Therefore, researchers used New Zealand rabbits to prepare a blood-purifying animal model in subsequent studies, and again verified that New Zealand rabbits are ideal experimental animals for blood-purifying treatment. In the research, the internal carotid artery is cut, a central venous catheter is arranged, the drainage blood flows back from the external jugular vein at the same side to the internal tube arrangement mode, the vital sign of the experimental rabbit in operation is stable, but the infection rate of the wound after operation is high, the limb paralysis and the weight of the experimental rabbit are reduced, the death rate of the experimental rabbit after single whole blood perfusion treatment is 50-75%, and the data influence of the death of the rabbit on the research can be only compensated by increasing the number of the experimental rabbits. We summarize that the reasons for the non-ideal carotid artery dissection tube placement effect are: (1) After ligating the internal carotid artery, the perfusion volume of the arterial blood supply area can be reduced sharply in a short time, and the physiological function of the tissue ischemia after the operation is limited; (2) The ligatured blood vessel has fine disuse property and scar healing of wound tissues after operation, and the secondary catheterization of the in-situ blood vessel is difficult; (3) The experimental animals are frequently listless, weight loss or quadriplegia after operation, and the postoperative infection and mortality are high; (4) Survivors also need a longer recovery period to accept the secondary experiment, and most experimental rabbits die after the secondary experiment; (5) The number of the early-stage experimental rabbits must be increased to complete the later-stage research work, so that the experimental cost is greatly increased.
In recent years, a new direction of research on a tube placing path and a tube placing mode of an experimental animal is to use a double-cavity catheter, perform arterial or venous puncture tube placing by using a Seldinger improved puncture method, and flow back one cavity of blood into the experimental animal body through the other cavity after passing through a blood purifier, so that the death rate of the experimental animal is reduced, and the utilization rate of the experimental animal is improved. However, part of congenital defects are still difficult to overcome, and the method mainly aims at (1) separating out target blood vessels by using glass needles in the operation, has large wounds and has higher postoperative wound infection rate; (2) The puncture blood vessel after operation can not be completely closed, and the problem is particularly remarkable when arterial puncture is performed and the diameter of a catheter is thicker, and the puncture blood vessel still needs to be ligated when necessary, so that the physiological function of an experimental animal after operation is affected; (3) The ligatured vascular animal can not puncture the catheter again at the same position within a short period of time; (4) There is still some death of the experimental animals, increasing experimental cost and uncontrollable study.
In conclusion, an animal model which is safe, effective, reusable, simple in experimental operation, relatively low in cost and easy to replicate and popularize is established, and is an important point and a difficult point of basic research of the current blood purification treatment.
Disclosure of Invention
The invention aims to provide a safe, effective and reusable blood purification animal model, and particularly relates to a blood purification rabbit model, and a construction method and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a construction method of a blood purification rabbit model, which comprises the following steps:
a closed needle-prick-prevention venous indwelling needle is adopted for carrying out rabbit vein or artery puncture catheterization, and a blood purification rabbit model is established;
the path of the tube is an internal carotid artery puncture tube, a femoral artery puncture tube, an internal jawbone vein puncture tube or an ear central artery puncture tube.
Preferably, the method for placing the carotid artery puncture tube comprises the following steps:
making 1.5-2.5 cm incision along the midline downwards at the thyroid cartilage of the neck, and respectively placing the indwelling needle into the internal carotid artery and the external jugular vein, wherein the internal carotid artery is used as a perfusion end, and the external jugular vein is used as a drainage end.
Preferably, the femoral artery puncture catheter placement method comprises the following steps:
the groin is longitudinally cut into 1.5-2.5 cm openings, the indwelling needle is respectively arranged on the right femoral artery and the right femoral vein, the right femoral artery is arranged to serve as a perfusion end, and the right femoral vein is arranged to serve as a drainage end.
Preferably, the method for the intra-jaw intravenous puncture catheterization comprises the following steps: an incision of 1.5-2.5 cm is made downwards along the normal midline at the thyroid cartilage of the neck, the indwelling needle is respectively arranged in the intra-jaw vein and the extra-jaw vein, the intra-jaw vein is used as a perfusion end, and the extra-jaw vein is used as a drainage end.
Preferably, the method for the central auricular artery puncture catheterization comprises the following steps:
the indwelling needle is used for respectively placing the tube in the central auricle and the auricular veins, the central auricle is used as a perfusion end, and the auricular veins are used as a drainage end.
Preferably, the indwelling needle is 22G in type.
The invention also provides a blood purification rabbit model constructed by the construction method.
The invention also provides application of the construction method in establishing a blood purification treatment animal model.
The invention also provides application of the blood purification rabbit model in researching animal blood purification.
The invention provides a blood purification rabbit model, and a construction method and application thereof. The invention provides a new Zealand rabbit blood purification model established by using the closed puncture-proof venous indwelling needle for carotid artery puncture catheterization, femoral artery puncture catheterization or maxillo-mandibular vein puncture catheterization or auricular central artery catheterization for the first time, and provides guidance and reference for later blood purification treatment.
The method proves that the New Zealand rabbits are ideal experimental animals for blood purification treatment, and provides reference and guidance for the selection of basic research animals in the future.
The invention adopts the closed type puncture-proof vein indwelling needle to carry out arterial and vein puncture catheterization, and experiments show that the catheterization method is feasible, safe and sustainable, and provides a new catheterization method for the research of later blood purification.
The tube placing method has high safety, simple tube placing, simple operation surrounding management and sustainable utilization. And reliable data support is provided for the basic research of later blood purification.
The success rate is high: the central auricular artery puncture tube, the internal carotid artery puncture tube and the intrajawbone vein puncture tube and the femoral artery puncture tube can be successfully placed, and the operation is simple and convenient, and the popularization is easy.
The repeated use rate is high: the 4 tube placing modes can be repeatedly used, and the repetition frequency is 9.0-10.3 times.
The death rate is low: the 4 tube placing modes have no death of New Zealand rabbits.
The physiological influence on the organism is small: 4 tube placing modes have no influence on the blood convention, blood sugar, electrolyte and liver functions of New Zealand rabbits.
Drawings
Fig. 1 is a schematic diagram of a blood purification rabbit model constructed by ear central artery puncture catheterization.
Fig. 2 is a schematic diagram of a blood purification rabbit model constructed by internal carotid artery vein puncture catheterization.
Figure 3 neck artery anatomy of New Zealand rabbits.
Fig. 4 shows a state of a new zealand rabbit after a closed puncture-proof venous indwelling needle performs central artery puncture catheterization.
Fig. 5 shows a state of a new zealand rabbit immediately after a closed needle-stick injury prevention venous indwelling needle performs carotid artery, femoral artery and maxillo-venous puncture catheterization.
FIG. 6 is a route pattern created by the blood purification rabbit model of examples 1 to 4.
Detailed Description
The invention provides a construction method of a blood purification rabbit model, which comprises the following steps:
a closed needle-prick-prevention venous indwelling needle is adopted for carrying out rabbit vein or artery puncture catheterization, and a blood purification rabbit model is established; the path of the tube is an internal carotid artery puncture tube, a femoral artery puncture tube, an internal jawbone vein puncture tube or an ear central artery puncture tube.
In the invention, the method for placing the carotid artery puncture tube comprises the following steps: making 1.5-2.5 cm incision along the midline downwards at the thyroid cartilage of the neck, and respectively placing the indwelling needle into the internal carotid artery and the external jugular vein, wherein the internal carotid artery is used as a perfusion end, and the external jugular vein is used as a drainage end. In the present invention, the size of the incision is preferably 2.0cm. The method for placing the carotid artery comprises the following steps: the right hand holds a 22G closed type vein indwelling needle capable of preventing puncture, namely, the 22G indwelling needle enters the needle from the distal end of the carotid artery to the proximal end, and after successful puncture, the needle core is withdrawn, the indwelling needle is fixed, and the infusion tube is connected. External jugular vein catheterization method: the right hand 22G indwelling needle enters the needle from the distal end of the external jugular vein to the proximal end, after successful puncture, the needle core is withdrawn, the indwelling needle is fixed, and the tail end of the external jugular vein indwelling needle is connected with the drainage tube. 1250IU/ml heparin sodium physiological saline 2ml/Kg is injected from the external jugular vein to perform in vivo anticoagulation, a peristaltic pump is started, the pump speed is controlled to be 5ml/min, and when blood runs to a vein pipeline, a drainage tube is opened to enable the blood to flow back into the body. After operation, the remaining needle and the puncture are pulled out, and the pressure hemostasis is carried out for 10min.
In the invention, the femoral artery puncture catheter placement method comprises the following steps:
the groin is longitudinally cut into 1.5-2.5 cm openings, and the 22G indwelling needle is respectively arranged on the right femoral artery and the right femoral vein, the right femoral artery is arranged as a perfusion end, and the right femoral vein is arranged as a drainage end. In the present invention, the size of the inguinal longitudinal incision is preferably 2cm. In the invention, the method for placing the right femoral artery comprises the following steps: the right hand 22G indwelling needle is inserted from the distal end of the femoral artery to the proximal end, and after successful puncture, the needle core is withdrawn and the indwelling needle is fixed, and the indwelling needle is connected with the perfusion tube. The method for placing the right femoral vein tube comprises the following steps: the right hand 22G indwelling needle is inserted from the distal end of the femoral vein on the right side to the proximal end, after successful puncture, the needle core is withdrawn, the indwelling needle is fixed, and the tail end of the femoral vein indwelling needle is connected with the drainage tube. After the femoral vein is placed into a tube, 1250IU/ml heparin sodium physiological saline is injected into 2ml/Kg of the femoral vein to perform in-vivo anticoagulation, a peristaltic pump is started, the pump speed is controlled to be 5ml/min, and when blood runs to a vein pipeline, a drainage tube is opened to enable the blood to flow back into the body. After operation, the remaining needle and the puncture are pulled out, and the pressure hemostasis is carried out for 10min.
In the invention, the method for the intra-jaw intravenous puncture catheterization comprises the following steps: an incision of 1.5-2.5 cm is made downwards along the midline at the thyroid cartilage of the neck, and the 22G indwelling needle is respectively arranged in the intra-jaw vein and the extra-jaw vein, wherein the intra-jaw vein is used as a perfusion end, and the extra-jaw vein is used as a drainage end. In the present invention, the size of the incision is preferably 2cm. The method for placing the tube in the jaw vein comprises the following steps: the right hand 22G indwelling needle is inserted from the distal end of the intrajawbone vein to the proximal end, and after successful puncture, the needle core is withdrawn and the indwelling needle is fixed, and the indwelling needle is connected with the perfusion tube. The method for placing the external jaw vein tube comprises the following steps: the right hand 22G indwelling needle is inserted from the distal end of the extra-mandibular vein to the proximal end, after successful puncture, the needle core is withdrawn and the indwelling needle is fixed, and the tail end of the extra-mandibular vein indwelling needle is connected with the drainage tube. After the external jaw vein is placed into a tube, 1250IU/ml heparin sodium physiological saline 2ml/Kg is injected for in vivo anticoagulation, a peristaltic pump is started, the pump speed is controlled to be 5ml/min, and when blood runs to a vein pipeline, a drainage tube is opened to enable the blood to flow back into the body. After operation, the remaining needle and the puncture are pulled out, and the pressure hemostasis is carried out for 10min.
In the invention, the method for the central auricular artery puncture catheterization comprises the following steps:
the indwelling needle is used for respectively placing a tube in the auricular vein and the auricular central artery, the auricular central artery is used as a perfusion end, and the auricular vein is used as a drainage end. The right hand medical 22G closed type vein indwelling needle capable of preventing needle puncture from entering the needle from the distal end of the vein at the auricular edge to the proximal end, after successful puncture, the needle core is withdrawn, the indwelling needle is fixed, after blood flows out, blood is taken out to be measured, 1250IU/ml heparin sodium physiological saline 2ml/Kg is slowly pushed into the indwelling needle for whole body heparinization, and the tail end of the indwelling needle is connected with the drainage tube. The central artery is positioned in the center of the rabbit ear, the pipe diameter is thicker, the color is more bright red, the puncture of the central artery of the ear, the placement of the pipe and the perfusion pipe are finished by the same operation, the perfusion pipe is connected to the peristaltic pump, the pump speed is controlled to be 5ml/min, and when blood moves to the venous pipeline, the drainage pipe is opened and connected with the venous pipeline, so that the blood flows back into the body. After operation, the remaining needle and the puncture are pulled out, and the pressure hemostasis is carried out for 5min.
In the invention, the model of the indwelling needle is 22G.
The invention also provides a blood purification rabbit model constructed by the construction method.
The invention also provides application of the construction method in establishing a blood purification treatment animal model.
The invention also provides application of the blood purification rabbit model in researching animal blood purification.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
The animals of the embodiment of the invention are healthy and white New Zealand rabbits, the weight is 2.5-3.0 kg, and 30 experimental rabbits and male and female halves are needed for each tube placing mode.
The data of the invention are analyzed by SPSS22.0 statistical software. Normal distribution of measurement data in mean ± standard deviationThe two-group comparison uses two independent sample t test, the multi-group comparison uses single factor analysis of variance, and the further two-group comparison uses Tukey-Kramer test. The number of the count data (percentage) [ n (%)]X represents the comparison between groups using the list data 2 Checking, further comparing two by two with X 2 A segmentation method; when the theoretical frequency is less than 5,then Fisher exact probability method is used. With P<A difference of 0.05 is statistically significant.
Example 1
Blood purification rabbit model constructed by central auricular artery puncture catheter
(1) Preoperative preparation: 2 sterile infusion apparatuses are adopted, the tail end of each infusion apparatus is cut off, and each infusion apparatus is 20cm long and is used as a perfusion tube and a drainage tube respectively. The experimental rabbits were fasted for 12 hours before operation, and were not forbidden. (2) anesthesia: 3% pentobarbital sodium 30mg/Kg, from the left side margin vein slow static pushing, later according to the experimental rabbit reaction condition, can static pushing the total dose of 1/5, so as to maintain the anesthesia depth. (3) surgery: gently removing hairs on skin on the surface of a blood vessel of a right ear of a rabbit, flicking an eardrum, smearing the skin of the right ear with 75% alcohol for 3 times, fixing the rabbit ear by a left hand, holding a medical 22G closed type puncture-preventing venous indwelling needle (22G indwelling needle) by the right hand, feeding the needle from the distal end of a vein at the edge of the ear to the proximal end, withdrawing a needle core and fixing the indwelling needle after successful puncture, taking blood to be measured after blood flows out, slowly pushing 1250IU/ml heparin sodium physiological saline into the indwelling needle for 2ml/Kg whole body heparinization, and connecting the tail end of the indwelling needle with a drainage tube. The central artery is positioned in the center of the rabbit ear, the pipe diameter is thicker, the color is more bright red, the puncture of the central artery of the ear, the placement of the pipe and the perfusion pipe are finished by the same operation, the perfusion pipe is connected to the peristaltic pump, the pump speed is controlled to be 5ml/min, and when blood moves to the venous pipeline, the drainage pipe is opened and connected with the venous pipeline, so that the blood flows back into the body. After operation, the remaining needle is pulled out, the puncture is pressurized to stop bleeding for 5min, and the puncture is aseptically processed. A schematic diagram of the rabbit model constructed by the method is shown in FIG. 1.
After the construction is completed, the state of the New Zealand rabbits can be photographed and observed, as shown in FIG. 4, wherein the image is obtained by randomly selecting 1 from 30 models to photograph.
And (3) continuously carrying out auricular central arterial puncture catheterization on the experimental rabbits for 24 hours to construct a blood purification rabbit model, recording the maximum repeatable catheterization times of single rabbits, recording wound infection and death, and recording the reasons of abandoning the experimental rabbits after the maximum times of catheterization. The remaining experimental rabbits were repeatedly operated and experimental data were recorded separately. The results are shown in Table 1.
Fig. 4 shows that the rabbit was in good condition after the blood purification rabbit model was prepared by the method.
Example 2
Blood purification rabbit model constructed by intra-jaw venipuncture catheterization
(1) Preparation of preoperative preparation and anesthesia of the rabbit model with central artery catheterization. (2) surgery: the rabbits were fixed on the operating table on their back. Shearing the neck to prepare the skin, sterilizing with iodophor, making a 2cm incision downwards along the midline from the thyroid cartilage of the neck, clamping the skin on the right side of the incision, lifting the skin from the back of the neck to the incision by the left finger, and making two branches of the external jugular vein visible on the outer edge of the mastoid muscle of the sternoclavicular bone, wherein the inner side is the internal jaw vein, the outer side is the external jaw vein, and the anatomic diagram is shown in figure 3. The wound is covered with normal saline gauze for 1min to stop bleeding. The operation does not destroy the fat layer and connective tissue at the periphery of the maxillo-and external-maxillo-veins, and the maxillo-and external-maxillo-veins are separated by a glass needle, and only the right and left internal-and external-maxillo-veins are exposed at the incision. The right hand of the operator holds the medical 22G indwelling needle from the distal end of the jawbone vein to the proximal end, and after successful puncture, the needle core is withdrawn and the indwelling needle is fixed, and the indwelling needle is connected with the perfusion tube. The same procedure was completed for left extra-mandibular vein catheterization. The external jaw vein is injected with 1250IU/ml heparin sodium physiological saline solution and 2ml/Kg of heparin sodium physiological saline solution for in vivo anticoagulation. After the perfusion tube is connected with the arterial pipeline, a peristaltic pump is started, the pump speed is controlled to be 5ml/min, and when blood flows to the venous pipeline, a drainage tube is opened and connected with the tail end of the extra-mandibular venous indwelling needle, so that the blood flows back into the body. After operation, the remaining needle and the puncture are pulled out, and the pressure hemostasis is carried out for 10min. After aseptic treatment, closing the window, and covering the wound with sterile gauze. The state of the rabbit after the operation by this method is shown in fig. 5.
Fig. 5 shows that the mental state of new zealand rabbits is slightly less optimal but without limb paralysis immediately after the intra-mandibular venipuncture catheterization. 24-48 hours after operation, the mental state and the diet water of the New Zealand rabbits are as usual, and the wound is free from infection. Fig. 5 is a photograph of a rabbit in a randomly selected blood purification model.
And (3) continuously carrying out intrajaw intravenous puncture catheterization on the experimental rabbits for 72 hours to construct a blood purification rabbit model, recording the maximum repeatable catheterization times of single rabbits, recording wound infection and death, and recording the reasons of abandoning the experimental rabbits after the maximum times of catheterization. The remaining experimental rabbits were repeatedly operated and experimental data were recorded separately. The results are shown in Table 1.
Example 3
Blood purification rabbit model constructed by carotid artery puncture catheter
(1) Preoperative preparation and anaesthesia the procedure of reference example 2. (2) intraoperative manipulation the catheterization method of reference example 2. The internal carotid artery is used as a perfusion end, and the external jugular vein is used as a drainage tube. After operation, the remaining needle and the puncture are pulled out, and the pressure hemostasis is carried out for 10min. After aseptic treatment, closing the window, and covering the wound with sterile gauze. A schematic of the construction is shown in fig. 2.
The method is used for constructing and obtaining 30 rabbit blood purification models. When the blood purification model is constructed by the method, no experimental rabbit dies during operation, the mental state of the experimental rabbit after operation is slightly low, the drinking water is slightly reduced, and the state of the experimental rabbit is consistent with that shown in fig. 5. 24-48 hours after operation, the mental state and the diet water of the New Zealand rabbits are recovered to be normal, the wound is not infected, and the experimental rabbits are free from limb paralysis and death.
And (3) continuously carrying out carotid artery puncture catheterization on the experimental rabbits for 72 hours to construct a blood purification rabbit model, recording the maximum repeatable catheterization times of single rabbits, recording wound infection and death, and recording the reasons of abandoning the experimental rabbits after the maximum times of catheterization. The remaining experimental rabbits were repeatedly operated and experimental data were recorded separately. The results are shown in Table 1.
Example 4
Blood purification rabbit model constructed by femoral artery puncture catheter
(1) Preoperative preparation and anesthesia were as in example 1. (2) After conventional skin preparation and local disinfection, a longitudinal incision is made at the groin for 2cm, a right femoral artery catheterization and a right femoral vein catheterization are respectively completed by using a 22G indwelling needle according to the indwelling needle method of the embodiment 1, the femoral artery catheterization is used as a perfusion end, heparin is injected for anticoagulation after the femoral vein catheterization, and the femoral vein catheterization is used as a drainage end, so that the blood drainage operation is completed. After operation, the remaining needle and the puncture are pulled out, and the pressure hemostasis is carried out for 10min. After aseptic treatment, closing the window, and covering the wound with sterile gauze.
The method is used for constructing a blood purification model of 30 rabbits, no experimental rabbits die during operation, the mental state and the diet water of the experimental rabbits after operation are slightly poor, and the state of the experimental rabbits is consistent with that shown in fig. 5. The mental state and the dietary water of the experimental rabbits are recovered to be normal 24-48 hours after operation. No experimental rabbit had wound infection, lower limb swelling, gangrene, paralysis and death after operation.
And (3) continuously carrying out femoral artery puncture catheterization on the experimental rabbits for 72 hours to construct a blood purification rabbit model, recording the maximum repeated catheterization times of single rabbits, recording wound infection and death, and recording the reasons of abandoning the experimental rabbits after the maximum times of catheterization. The remaining experimental rabbits were repeatedly operated and experimental data were recorded separately. The results are shown in Table 1.
Table 1 4 conditions of construction of New Zealand rabbit model for blood purification by tube-placing method
Note that: a p compared with the central artery group of the ear<0.05； b P compared to the femoral artery group<0.05; -: adopts Fisher exact probability method, and has no χ 2 Values.
Wound crusting, tissue hardening, vascular collapse, infection and death were discarded [ n (%) ] during the course of the experiment.
Table 1 shows that the 4-way-set rabbit model has low infection rate and no death.
Experimental example 1
Examples 1 to 4 a roadmap of a blood purification rabbit model was constructed as shown in fig. 6.
The grades of the blood purification rabbit models prepared in examples 1 to 4 were evaluated. The evaluation criteria are shown in tables 2 to 5.
TABLE 2 post-operative nerve function evaluation criteria for rabbit model
Table 3 rabbit model postoperative wound infection evaluation criteria
| Level of | Experimental Rabbit behavior |
| Level 0 | Wound cleansing |
| Level 1 | Mild swelling of skin and good blood circulation at wound |
| Level 2 | Red, swelling, tenderness and increased skin temperature of wound |
| 3 grade | Erosion and seepage of wound base |
| Grade 4 | Pus overflowed from wound |
| Grade 5 | Extensive tissue necrosis at the wound site |
TABLE 4 Rabbit model postoperative lower limb blood and motor ability evaluation criteria
TABLE 5 Rabbit model postoperative body function evaluation criteria
The rabbit model constructed in example 1 was evaluated according to the standard for nerve function, wound infection, lower limb blood and motor ability to be 0 level, and body function to be 1 level. The rabbit model constructed in examples 2 to 4 was rated 1 for nerve function, wound infection, lower limb blood and motor ability.
The blood index of the experimental rabbits in the blood purification rabbit model constructed in examples 1 to 4 was examined. The test results are shown in Table 6.
Table 6 4 blood detection index of blood purification New Zealand rabbit model constructed by tube-placing method
Note that: a p compared with the central artery group of the ear<0.05； b P compared to the femoral artery group<0.05; -: adopts Fisher exact probability method, and has no χ 2 Values.
Table 6 shows that the blood index in the rabbit model prepared by examples 1 to 4 is normal.
As can be seen from the above embodiments, the present invention provides a blood purification rabbit model, and a construction method and application thereof. The invention provides a new Zealand rabbit blood purification model established by using the closed puncture-proof venous indwelling needle for carotid artery puncture catheterization, femoral artery puncture catheterization or maxillo-mandibular vein puncture catheterization or auricular central artery catheterization for the first time, and provides guidance and reference for later blood purification treatment.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (9)
1. The construction method of the blood purification rabbit model is characterized by comprising the following steps of:
a closed needle-prick-prevention venous indwelling needle is adopted for carrying out rabbit vein or artery puncture catheterization, and a blood purification rabbit model is established;
the path of the tube is an internal carotid artery puncture tube, a femoral artery puncture tube, an internal jawbone vein puncture tube or an ear central artery puncture tube.
2. The method for constructing a carotid artery puncture catheter according to claim 1, wherein the method for constructing a carotid artery puncture catheter comprises the following steps:
making 1.5-2.5 cm incision along the midline downwards at the thyroid cartilage of the neck, and respectively placing the indwelling needle into the internal carotid artery and the external jugular vein, wherein the internal carotid artery is used as a perfusion end, and the external jugular vein is used as a drainage end.
3. The method for constructing a femoral artery puncture catheter according to claim 2, wherein the method comprises the following steps:
the groin is longitudinally cut into 1.5-2.5 cm openings, the indwelling needle is respectively arranged on the right femoral artery and the right femoral vein, the right femoral artery is arranged to serve as a perfusion end, and the right femoral vein is arranged to serve as a drainage end.
4. The method for constructing a cannula according to claim 3, wherein the method for positioning the cannula for intravenous puncture in the jaw comprises the following steps: an incision of 1.5-2.5 cm is made downwards along the normal midline at the thyroid cartilage of the neck, the indwelling needle is respectively arranged in the intra-jaw vein and the extra-jaw vein, the intra-jaw vein is used as a perfusion end, and the extra-jaw vein is used as a drainage end.
5. The method according to claim 4, wherein the method for placing the ear central artery puncture tube comprises the following steps:
the indwelling needle is used for respectively placing the tube in the central auricle and the auricular veins, the central auricle is used as a perfusion end, and the auricular veins are used as a drainage end.
6. The method according to any one of claims 2 to 5, wherein the indwelling needle is 22G in type.
7. The blood purification rabbit model constructed by the construction method according to any one of claims 1 to 6.
8. Use of the construction method according to any one of claims 1 to 6 for the construction of a blood purification treatment animal model.
9. Use of the blood purification rabbit model of claim 7 for studying animal blood purification.
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