CN117099841A - Application of preservative composite paper in Morchella preservation - Google Patents
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- 241000221638 Morchella Species 0.000 title claims abstract description 85
- 239000002131 composite material Substances 0.000 title claims abstract description 36
- 230000002335 preservative effect Effects 0.000 title claims abstract description 29
- 239000003755 preservative agent Substances 0.000 title claims abstract description 28
- 238000004321 preservation Methods 0.000 title claims abstract description 10
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- 229920002678 cellulose Polymers 0.000 claims abstract description 30
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000000725 suspension Substances 0.000 claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 8
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- 238000003756 stirring Methods 0.000 claims abstract description 6
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- 238000002156 mixing Methods 0.000 claims abstract 2
- 239000012528 membrane Substances 0.000 claims description 5
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- 235000011187 glycerol Nutrition 0.000 claims 2
- 238000000034 method Methods 0.000 abstract description 10
- 230000008901 benefit Effects 0.000 abstract description 8
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- 235000013305 food Nutrition 0.000 abstract description 4
- 238000004806 packaging method and process Methods 0.000 abstract description 4
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 25
- 229940118019 malondialdehyde Drugs 0.000 description 25
- 230000000844 anti-bacterial effect Effects 0.000 description 24
- 102000030523 Catechol oxidase Human genes 0.000 description 22
- 108010031396 Catechol oxidase Proteins 0.000 description 22
- 102000016938 Catalase Human genes 0.000 description 18
- 108010053835 Catalase Proteins 0.000 description 18
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- 235000002779 Morchella esculenta Nutrition 0.000 description 17
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- 239000002250 absorbent Substances 0.000 description 12
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- 230000000694 effects Effects 0.000 description 9
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- 239000000126 substance Substances 0.000 description 4
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- 102000004190 Enzymes Human genes 0.000 description 2
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- 241001660097 Pedobacter Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
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- 239000012298 atmosphere Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 230000015572 biosynthetic process Effects 0.000 description 1
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- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
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- 238000011049 filling Methods 0.000 description 1
- 239000007888 film coating Substances 0.000 description 1
- 238000009501 film coating Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229930003658 monoterpene Natural products 0.000 description 1
- 150000002773 monoterpene derivatives Chemical class 0.000 description 1
- -1 monoterpenes Chemical class 0.000 description 1
- 235000002577 monoterpenes Nutrition 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
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- 239000005416 organic matter Substances 0.000 description 1
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- 244000052769 pathogen Species 0.000 description 1
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- 229920006254 polymer film Polymers 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of preservative composite paper in Morchella preservation; the fresh-keeping composite paper is prepared by adding the emulsifiable concentrate oil into carboxylated cellulose nano whisker suspension, then adding glycerol, stirring and uniformly mixing, performing ultrasonic treatment, filtering, soaking the water-absorbing paper in the filtered solution, and airing; when in use, the Morchella is separated and placed layer by using the fresh-keeping compound paper, and the Morchella is refrigerated and kept fresh; the preservative composite paper prepared by the invention is used for packaging Morchella, is simple and convenient to operate, effectively prolongs the preservative storage period of Morchella, has high economic benefit, is suitable for transportation and storage processes of various Morchella, is suitable for long-distance or long-time transportation and storage or short-distance transportation and storage, can effectively prolong the shelf life of Morchella by 7-14 days, and ensures the food safety.
Description
Technical Field
The invention relates to preparation of novel preservative composite paper and application of the novel preservative composite paper in Morchella preservation, and belongs to the technical field of food packaging and preservation.
Background
Morchella has unique flavor and extremely high nutritional value, is more and more popular worldwide, and has powerful functional properties such as antioxidant and anti-inflammatory biological activity, antibacterial, antiproliferative and potential anticancer agents. However, morchella is extremely susceptible to spoilage by microorganisms after picking, during transportation and storage, so that the nutritional value and the commodity value are reduced, and the shelf life is shortened.
At present, the method for preserving Morchella at home and abroad mainly comprises the following steps: the drying method has the advantages of low equipment cost, easy operation and wide application, and the drying method has the advantages of high equipment cost and low efficiency. The drying method has great influence on the nutrition loss of Morchella and the flavor. The film coating technology is characterized in that a layer of polymer film with antibacterial effect is uniformly coated on the surface of Morchella, so that the moisture loss is reduced, the respiration is blocked, a certain antibacterial effect is achieved, and the loss of nutrient components during storage is reduced; however, as the Morchella has a plurality of holes on the surface, the Morchella is not easy to dry after being coated, and a moist environment which is conducive to the growth and propagation of harmful microorganisms is provided for the transportation and storage process; and is not easy to be cleaned before eating; the modified atmosphere preservation technology can inhibit the respiration of the picked fruit and vegetable products and reduce the physiological metabolism activity by adjusting the content of the gas component in the fruit and vegetable preservation space, thereby achieving the purpose of preservation; the method is only suitable for large-scale transportation of Morchella, and has been researched and found to use CO 2 When the gas is fresh-keeping, when CO 2 When the volume fraction exceeds 30%, morchella has whitening phenomenon, and generates peculiar smell. Therefore, the inert gas and the gas content can have influence on Morchella. Exogenous energy compound ATP treatment, through increasing intracellular ATP content and energy charge level, reduces active oxygen formation, maintains energy balance, reduces Morchella respiratory rate and organic matter consumption, thereby inhibiting microorganism growth, reducing sugar, and reducing lipid oxidation degree. The fresh-keeping effect is good, but the industrialization is difficult at present due to the technical difficulty and cost. The combination of slightly acidic electrolyzed water and ultraviolet light for fresh keeping has strong antibacterial activity, no adverse effect on human health, and the combination of the slightly acidic electrolyzed water and ultraviolet light has a certain effect on the fresh keeping of Morchella, but has no fresh keeping effect when the ultraviolet light is applied to inhibiting the microbial spoilage on the surfaces of fruits and vegetablesLong, suitable for short-distance fresh-keeping, and the technical equipment is expensive, and can not be used on a small scale.
Cellulose nanowhisker is a natural biomass nanomaterial, has natural affinity to composite materials, can form new bonds to reconnect through breaking original bonds, so that the polymer and the filling material have stronger adhesive strength, and have the advantages of high strength, high crystallinity, higher stability, biocompatibility, biodegradability and the like, and are often used as reinforcing agents of the composite materials. The cellulose nano whisker modified by carboxylation has the advantages of more easily forming new bonds, better biocompatibility, extremely strong thixotropic property, better water dispersion system, larger viscosity to the system and the like due to the fact that the surface of the cellulose nano whisker contains carboxyl.
The emulsifiable concentrate is natural essential oil obtained from resin, has been used for thousands of years, is widely applied to treating ulcers, wounds and the like, and has a strong antibacterial effect due to the fact that the main component of the emulsifiable concentrate contains compounds such as monoterpenes, ketols and the like.
Disclosure of Invention
The invention provides a preparation method of preservative composite paper and application thereof in Morchella preservation, firstly, carboxylated cellulose nanowhiskers are prepared into suspension by a high-pressure homogenizer, and then essential oil is used as a natural antibacterial substance and added into the suspension to prepare an antibacterial system; then, soaking the water-absorbing paper in an antibacterial system, and airing at room temperature to obtain composite paper; when in use, the Morchella is separated and placed layer by using the fresh-keeping compound paper, and is put into a foam box for refrigeration and fresh keeping.
The invention separates and identifies main putrefying bacteria in the cold storage process of Morchella, wherein the bacteria are mainly Pseudomonas, pedobacter and Flavobacterium, and the fungi are mainly Cystofilobasidium and Gibberella; the packaging material with more antibacterial effect is obtained by developing fresh-keeping composite paper aiming at the main spoilage bacteria.
The preparation method of the fresh-keeping composite paper comprises the following steps:
(1) Adding the essential oil emulsion into the carboxylated cellulose nanowhisker suspension according to the proportion of adding 100mL of deionized water into 1-2 g of carboxylated cellulose nanowhisker, homogenizing for 25 times in a high-pressure homogenizer at 800bar to homogenize the carboxylated cellulose nanowhisker uniformly to obtain the carboxylated cellulose nanowhisker suspension with the mass volume concentration of 1-2%;
(2) Adding the emulsion essential oil into the carboxylated cellulose nano whisker suspension in the step (1), adding glycerol as a lubricant, magnetically stirring for 20-25 min, performing ultrasonic treatment for 2-4 times for 5min each time, and filtering by adopting a filter membrane with the pore diameter of 0.45 mu m to prepare an antibacterial system; wherein the mass volume ratio g of the emulsion essential oil and the carboxylated cellulose nano whisker suspension is 0.1-0.5% of mL, and the mass volume ratio g of the glycerol and the carboxylated cellulose nano whisker suspension is 4-6% of mL;
(3) Soaking 20cm multiplied by 100cm of absorbent paper in each 100mL of antibacterial system, soaking the absorbent paper in the antibacterial system in the step (2) for 25-35 min, and drying at normal temperature to obtain fresh-keeping composite paper; and (3) separating Morchella by using fresh-keeping compound paper, placing the Morchella layer by layer, putting the Morchella into a foam box, and refrigerating and preserving.
The preservative composite paper takes the packaging absorbent paper as a substrate, not only can absorb moisture of Morchella esculenta in the transportation and storage period, but also has a certain buffering effect and a protective effect on Morchella esculenta; the olibanum essential oil is used as an antibacterial substance, is safe and nontoxic, and plays a certain role in inhibiting the growth and reproduction of microorganisms. The carboxylated cellulose nano whisker is used as an emulsifiable concentrate carrier, is biodegradable, has good biocompatibility and strong adhesive strength, can better load antibacterial substances, and has high strength and high stability.
Compared with the prior art, the invention has the following advantages:
(1) The preservative composite paper prepared by the invention is used for packaging Morchella, is simple and convenient to operate, effectively prolongs the preservative storage period of Morchella, has high economic benefit, is suitable for long-distance or long-time transportation and storage or short-distance transportation and storage of various Morchella, is suitable for small-dose transportation and storage, can effectively prolong the shelf life of Morchella, can prolong 7-14 days, and ensures the food safety;
(2) The fresh-keeping composite paper prepared by the invention can effectively inhibit the growth and propagation of microorganisms in the transportation and storage process of the toadstool after picking, and particularly has a key effect on the inhibition of main putrefying bacteria and the enzyme activity; effectively protecting and maintaining physical and chemical indexes and nutritional ingredients of Morchella;
(3) The preservative composite paper prepared by the invention can widen the sales market of Morchella, reduce the transportation cost and avoid the economic loss of more Morchella caused by spoilage;
(4) The preparation method of the fresh-keeping composite paper provided by the invention has the advantages of simple process, low cost, environment friendliness and safety.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but the scope of the present invention is not limited to the above description, and the organic solvents used in the examples are all of analytical purity of domestic origin; the hardness of Morchella is determined by using TA.TOUCH (China ultra technology instruments Co., ltd.), the microbial community change is detected by using a kit purchased from Nanjing established biotechnology Co., ltd.) after total DNA is extracted by using a genome DNA extraction kit (Shanghai biological engineering Co., ltd.) and amplified by using a PCR instrument (Ningbo New Zhi Biotech Co., ltd.) and then sequenced by Shanghai biological Co., ltd., antioxidant enzyme (PPO, CAT) activity.
Example 1: preparation of preservative composite paper and application of preservative composite paper in storage process of Morchella
(1) Adding 100mL of deionized water into 2g of carboxylated cellulose nanowhisker, homogenizing for 25 times in a high-pressure homogenizer at 800bar to homogenize the carboxylated cellulose nanowhisker uniformly to obtain carboxylated cellulose nanowhisker suspension with the concentration of 2%;
(2) Adding 0.1g of essential oil into the carboxylated cellulose nano whisker suspension in the step (1), adding 5g of glycerol, stirring for 20min at the speed of 1200r/min on a magnetic stirrer, performing ultrasonic treatment for 5min, repeating for three times to fully load the essential oil, and filtering with a filter membrane with the pore diameter of 0.45 mu m to obtain an antibacterial system;
(3) Soaking the water-absorbing paper in the antibacterial system of the step (2) for 30min according to the proportion of 20cm multiplied by 100cm water-absorbing paper in each 100mL antibacterial system, and drying for 12 hours at normal temperature to obtain the fresh-keeping composite paper;
(4) Separating Morchella by using the prepared fresh-keeping compound paper, placing the Morchella layer by layer, putting the Morchella into a foam box, and refrigerating and keeping fresh; changes in the indices of Morchella were recorded during days 0, 4, 8, 16; meanwhile, the Morchella is packaged by absorbent paper which is not treated by an antibacterial system as a control;
the results showed that Morchella using untreated absorbent paper accounted for 45.32% of the population by bacterial Pseudomonas on day 0. Second are Pedobacterium (27.18%) and Flavobacterium (18.26%); on day 16 Pseudomonas is 78.7% and is the predominant pathogen in spoilage, and Pedobacterium (7.83%) and Flavobacterium (5.62%) are reduced in relative abundance during spoilage. The highest content of the 0 th natural fungus Morchella is 98.4% of the whole community, the highest content of the 0 th natural fungus Morchella is fruiting body of Morchella, other fungi are less than 1%, at the moment, the flora is stable, and the influence of microorganisms is small. Whereas Cystofilobasium increased with increasing days of storage, increasing to 9.50% from day 0 to day 16, gibberella accounted for 4.21%. While Morchella as a fruiting body only accounts for 65.31%, which means that the fruiting body is seriously affected by microorganisms and Morchella is seriously spoiled.
For hardness, day 0, morchella esculenta handles 32.71+ -0.42N, morchella esculenta caps 47.56+ -0.67N. By 16 days, morchella esculenta 13.09+ -0.77N, morchella esculenta 12.29+ -0.21N. Morchella is obviously softened and spoiled seriously. At this time, the food is completely spoiled, has a pungent odor and cannot be eaten. The changes in the content of PPO, CAT and Malondialdehyde (MDA) are shown in table 1. Wherein polyphenol oxidase (PPO) is activated by substrate when physiological disorder or tissue damage occurs in tissue, so that the content of PPO increases rapidly with days during storage, which indicates that the content of PPO is greatly affected by microorganisms and decay is accelerated. The decrease started by day 12, indicating that Morchella had completely spoiled. Whereas Catalase (CAT) has antioxidant activity, also increases with increasing days of storage in the early period of spoilage, and decreases rapidly by the highest day, 16 th day, to the eighth day; indicating that the morchella is completely spoiled and deteriorated in 8 th to 12 th days; malondialdehyde is an important index of damage to plant somatic cells, and is also a 3-class carcinogen published by the world health organization International cancer research institute, and the content thereof increases rapidly with time.
TABLE 1 variation of PPO, CAT and Malondialdehyde (MDA) content of Morchella using untreated absorbent paper
| Day 0 | Day 4 | Day 8 | Day 12 | Day 16 | |
| PPO(U/g) | 14.33 | 26.67 | 82.67 | 169.33 | 139.33 |
| CAT(×10 4 U/kg) | 99.02 | 218.18 | 489.25 | 342.79 | 134.64 |
| MDA(mmol/kg) | 11.22 | 42.31 | 32.45 | 57.99 | 80.65 |
。
The Morchella using the preservative composite paper accounts for 63.48% of the total weight of the Morchella by 16 days, and the relative abundance of Pedobacterium (4.31%) and Flavobacterium (6.32%) is reduced relative to that of Morchella packaged by untreated absorbent paper, so that the species abundance is relatively uniform and the structure is relatively stable. The fungi had a Morchella ration of 92.19% on day 16, indicating that the fruiting body remained well-conditioned. Cystofilobasium and Gibberella at 3.49% and 4.16%, respectively; for hardness, 22.12+/-0.40N of Morchella esculenta handles and 18.77+/-0.63N of Morchella esculenta caps are obtained after 16 days, and the hardness is obviously higher than that of Morchella esculenta packaged by untreated absorbent paper. At this time, morchella is not completely spoiled and can be eaten. The changes of the contents of PPO, CAT and Malondialdehyde (MDA) are shown in table 2, compared with the changes, the preservative composite paper obviously reduces the PPO and MDA of Morchella, especially the PPO, by about 2 times on 8 th and 12 th days, and still shows an increasing trend by 16 th days, which indicates that the Morchella is not completely rotten yet, but obviously increases the CAT value, which indicates that the preservative composite paper can achieve a good preservative effect by positively acting on the internal environment of tissue cells.
TABLE 2 variation of PPO, CAT and Malondialdehyde (MDA) content of Morchella using preservative paper
| Day 0 | Day 4 | Day 8 | Day 12 | Day 16 | |
| PPO(U/g) | 13.67 | 17.33 | 44.33 | 85.33 | 104.33 |
| CAT(×10 4 U/kg) | 99.23 | 284.32 | 527.44 | 493.56 | 301.49 |
| MDA(mmol/kg) | 12.03 | 17.26 | 25.17 | 25.23 | 42.24 |
。
Example 2: preparation of preservative composite paper and application of preservative composite paper in storage process of Morchella
(1) Adding 100mL of deionized water into 1.5g of carboxylated cellulose nanowhisker, homogenizing in a high-pressure homogenizer for 25 times at 800bar to homogenize the carboxylated cellulose nanowhisker uniformly to obtain carboxylated cellulose nanowhisker suspension with the concentration of 1.5%;
(2) Adding 0.3g of essential oil into the carboxylated cellulose nano whisker suspension in the step (1), adding 5g of glycerol, stirring for 20min at the speed of 1200r/min on a magnetic stirrer, performing ultrasonic treatment for 5min and repeating for three times to fully load the essential oil of the frankincense, and filtering with a filter membrane with the pore diameter of 0.45 mu m to obtain an antibacterial system;
(3) Soaking the water-absorbing paper in the antibacterial system of the step (2) for 30min according to the proportion of 20cm multiplied by 100cm water-absorbing paper in each 100mL antibacterial system, and drying for 12 hours at normal temperature to obtain the fresh-keeping composite paper;
(4) Separating Morchella by using the prepared fresh-keeping compound paper, placing the Morchella layer by layer, putting the Morchella into a foam box, and refrigerating and keeping fresh; changes in the indices of Morchella were recorded during days 0, 4, 8, 16; comparison is as in example 1;
morchella using the preservative composite paper has 71.52 percent of Pseudomonas, 7.22 percent of Pedobacterium and 5.48 percent of Flavobacterium on the 16 th day, and has reduced relative abundance, changed species abundance and stable structure compared with Morchella packaged by untreated absorbent paper. The 16 th natural fungus Morchella accounts for 91.77%, and the Cystofilobasidium accounts for 1.23% and the Gibberella accounts for 3.18%, respectively, which indicates that the fruiting body is not seriously damaged yet; for hardness, 24.42±0.61N for morchella, 20.91±1.21N for 16 days; the hardness of the Morchella esculenta on day 16 is obviously higher than that of the Morchella esculenta packaged by untreated absorbent paper, which indicates that the Morchella esculenta still maintains basic physicochemical properties, and is not completely spoiled at the moment. The change of the contents of PPO, CAT and Malondialdehyde (MDA) is shown in Table 3, the fresh-keeping compound paper obviously reduces the PPO and MDA of Morchella, and the MAD is obviously reduced by 1.79 times on day 16. The CAT value is obviously increased, which indicates that the fresh-keeping compound paper can have a positive effect on the internal environment of tissue cells, so that the fresh-keeping effect is well influenced;
TABLE 3 variation of PPO, CAT and Malondialdehyde (MDA) content of Morchella using preservative paper
| Day 0 | Day 4 | Day 8 | Day 12 | Day 16 | |
| PPO(U/g) | 14.67 | 18.33 | 45.33 | 92.67 | 118.31 |
| CAT(×10 4 U/kg) | 98.25 | 334.14 | 548.78 | 553.65 | 376.42 |
| MDA(mmol/kg) | 13.24 | 19.46 | 28.44 | 24.78 | 45.12 |
。
Example 3: preparation of preservative composite paper and application of preservative composite paper in storage process of Morchella
(1) Adding 100mL of deionized water into 2g of carboxylated cellulose nanowhisker, homogenizing for 25 times in a high-pressure homogenizer at 800bar to homogenize the carboxylated cellulose nanowhisker uniformly to obtain carboxylated cellulose nanowhisker suspension with the concentration of 2%;
(2) Adding 0.5g of essential oil into the carboxylated cellulose nano whisker suspension in the step (1), adding 5g of glycerol, stirring for 20min at the speed of 1200r/min on a magnetic stirrer, performing ultrasonic treatment for 5min and repeating for three times to fully load the essential oil of the frankincense, and filtering by adopting an ultrafiltration membrane with the aperture of 0.45 mu m to obtain an antibacterial system;
(3) Soaking the water-absorbing paper in the antibacterial system of the step (2) for 30min according to the proportion of 20cm multiplied by 100cm water-absorbing paper in each 100mL antibacterial system, and drying for 12 hours at normal temperature to obtain the fresh-keeping composite paper;
(4) And (3) utilizing the prepared preservative composite paper to separate Morchella layers by layers, placing the Morchella layers into a foam box, and refrigerating and preserving. Changes in the indices of Morchella were recorded during days 0, 4, 8, 16; comparison is as in example 1;
morchella using the preservative composite paper has a ratio of 53.26% by day 16, pedobacterium (14.54%) and Flavobacterium (11.78%) increased relative to Morchella packaged in untreated absorbent paper, but the Pedobacterium and Flavobacterium decreased, however, the relative abundance was still not low, the community diversity was high, and the flora was more stable. The 16 th Eumycota Morchella was 93.51% in weight, indicating that the fruiting body was not severely destroyed. Cystofilobasium and Gibberella at 0.82% and 2.76%, respectively; effectively inhibit the growth and propagation of main pathogenic fungi. For hardness, until 16 days, the Morchella esculenta handles 27.68 +/-0.78N and the Morchella esculenta caps 22.45 +/-0.92N, the hardness is obviously higher than that of Morchella esculenta packaged by untreated absorbent paper, which indicates that the content of the emulsifiable concentrate has a larger influence on the quality of Morchella esculenta, and the Morchella esculenta is not completely spoiled at the moment and also has a better form. The change of the contents of PPO, CAT and Malondialdehyde (MDA) is shown in a table 4, compared with the change, the fresh-keeping compound paper obviously reduces the PPO and MDA of Morchella, reduces more than 2 times of PPO, especially MDA on 8 th and 12 th days, is more than 2 times lower on 4 th, 12 th and 16 th days, obviously improves the CAT value, especially improves the fresh-keeping effect of the fresh-keeping compound paper on Morchella on 16 th day compared with that of Morchella which is not treated;
TABLE 4 variation of PPO, CAT and Malondialdehyde (MDA) content of Morchella using preservative paper
| Day 0 | Day 4 | Day 8 | Day 12 | Day 16 | |
| PPO(U/g) | 14.00 | 12.67 | 39.33 | 67.67 | 87.33 |
| CAT(×10 4 U/kg) | 99.26 | 313.57 | 529.24 | 567.31 | 377.29 |
| MDA(mmol/kg) | 14.56 | 10.37 | 19.16 | 22.42 | 35.21 |
。
Claims (4)
1. An application of preservative composite paper in Morchella preservation;
the fresh-keeping composite paper is prepared by adding the emulsifiable concentrate oil into carboxylated cellulose nano whisker suspension, then adding glycerol, stirring and uniformly mixing, performing ultrasonic treatment, filtering, soaking the water-absorbing paper in the filtered solution, and airing;
when in use, the Morchella is separated and placed layer by using the fresh-keeping compound paper, and the Morchella is refrigerated and kept fresh.
2. The use according to claim 1, characterized in that: the mass-volume ratio of the emulsifiable essence oil to the carboxylated cellulose nano whisker suspension is 0.1-0.5 percent, and the mass-volume ratio of the glycerin to the carboxylated cellulose nano whisker suspension is 4-6 percent.
3. The use according to claim 1, characterized in that: the mass volume concentration of the carboxylated cellulose nano whisker in the carboxylated cellulose nano whisker suspension is 1-2%.
4. The use according to claim 1, characterized in that: the filtration was carried out with a filter membrane having a pore size of 0.45. Mu.m.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117384802A (en) * | 2023-12-04 | 2024-01-12 | 中国科学院昆明植物研究所 | Flavobacterium fluviale strain for inhibiting Morchella esculenta and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117384802A (en) * | 2023-12-04 | 2024-01-12 | 中国科学院昆明植物研究所 | Flavobacterium fluviale strain for inhibiting Morchella esculenta and application thereof |
| CN117384802B (en) * | 2023-12-04 | 2024-04-09 | 中国科学院昆明植物研究所 | Flavobacterium fluviale strain for inhibiting Morchella esculenta and application thereof |
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