CN117089488A - Lactobacillus plantarum DT88 and application thereof in preparation of micro-plastic adsorption and removal products - Google Patents
Lactobacillus plantarum DT88 and application thereof in preparation of micro-plastic adsorption and removal products Download PDFInfo
- Publication number
- CN117089488A CN117089488A CN202310941575.2A CN202310941575A CN117089488A CN 117089488 A CN117089488 A CN 117089488A CN 202310941575 A CN202310941575 A CN 202310941575A CN 117089488 A CN117089488 A CN 117089488A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- microplastic
- plantarum
- lactiplantibacillus
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000006024 Lactobacillus plantarum Species 0.000 title claims abstract description 90
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims abstract description 90
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims abstract description 90
- 238000002360 preparation method Methods 0.000 title claims description 34
- 229920003023 plastic Polymers 0.000 title claims description 10
- 239000004033 plastic Substances 0.000 title claims description 10
- 238000001179 sorption measurement Methods 0.000 title description 15
- 229920000426 Microplastic Polymers 0.000 claims abstract description 57
- 244000005700 microbiome Species 0.000 claims abstract description 9
- 230000000813 microbial effect Effects 0.000 claims description 23
- 235000013305 food Nutrition 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 9
- 239000003963 antioxidant agent Substances 0.000 claims description 8
- 230000003078 antioxidant effect Effects 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 5
- 238000009472 formulation Methods 0.000 claims description 3
- 239000003463 adsorbent Substances 0.000 claims description 2
- 239000000825 pharmaceutical preparation Substances 0.000 claims description 2
- 229940127557 pharmaceutical product Drugs 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000002000 scavenging effect Effects 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 10
- 239000002253 acid Substances 0.000 abstract description 10
- 239000003833 bile salt Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 6
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 6
- 230000004792 oxidative damage Effects 0.000 abstract description 6
- 239000006041 probiotic Substances 0.000 abstract description 6
- 235000018291 probiotics Nutrition 0.000 abstract description 6
- 238000009825 accumulation Methods 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 4
- 230000000529 probiotic effect Effects 0.000 abstract description 4
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 3
- 230000000968 intestinal effect Effects 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 20
- 230000001580 bacterial effect Effects 0.000 description 18
- 239000007788 liquid Substances 0.000 description 15
- 239000001963 growth medium Substances 0.000 description 9
- 239000006872 mrs medium Substances 0.000 description 9
- 230000004083 survival effect Effects 0.000 description 9
- 239000002609 medium Substances 0.000 description 8
- 239000004005 microsphere Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 235000006708 antioxidants Nutrition 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- 241000917009 Lactobacillus rhamnosus GG Species 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 229940059406 lactobacillus rhamnosus gg Drugs 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 241000251468 Actinopterygii Species 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 210000000813 small intestine Anatomy 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 235000015872 dietary supplement Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000015784 hyperosmotic salinity response Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 238000012353 t test Methods 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- OCZVHBZNPVABKX-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine;ethanol Chemical compound CCO.[O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 OCZVHBZNPVABKX-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 238000003794 Gram staining Methods 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 210000000941 bile Anatomy 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 229940099352 cholate Drugs 0.000 description 2
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 238000009631 Broth culture Methods 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 241000237903 Hirudo Species 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical compound C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
- 235000005811 Viola adunca Nutrition 0.000 description 1
- 240000009038 Viola odorata Species 0.000 description 1
- 235000013487 Viola odorata Nutrition 0.000 description 1
- 235000002254 Viola papilionacea Nutrition 0.000 description 1
- 238000011481 absorbance measurement Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940093761 bile salts Drugs 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 108010005400 cutinase Proteins 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013502 plastic waste Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000003642 reactive oxygen metabolite Substances 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Environmental & Geological Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Soil Sciences (AREA)
- Epidemiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the technical field of microorganisms, in particular to lactobacillus plantarum DT88 and application thereof in preparing products for adsorbing and removing microplastic. The lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 provided by the invention is preserved in the Guangdong province microorganism strain collection center, and the preservation number is GDMCC No:63626. the strain has better acid resistance and bile salt resistance, can resist the gastrointestinal tract environment, can efficiently adsorb the microplastic, accelerates the discharge and removal of the microplastic, has antioxidation capability, can reduce the oxidative damage caused by the accumulation of the microplastic, has the potential of being developed into an edible probiotic product, and has the effects of adsorbing the microplastic, accelerating the discharge of the microplastic and reducing the damage of the microplastic as intestinal bacteria in a human body.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to lactobacillus plantarum DT88 and application thereof in preparing products for adsorbing and removing microplastic.
Background
Plastic waste produced from plastic articles is broken down into tiny particles in the environment. Typically, plastic particles with a particle size of less than 5mm are Microplastic (MPs), wherein plastic particles with a particle size of less than 0.1 μm are nano-plastic (NPs). The microplastic is widely existed in air, water and soil, can be ingested by plankton, fish, birds and the like, finally enters human body through a food chain, not only causes environmental pollution, but also forms potential threat to human health. Numerous studies have demonstrated that microplastic can cause damage to the digestive, respiratory, immune, nervous and reproductive systems of rodents and aquatic organisms, that microplastic accumulation in tissues cannot be cleared, can cause a substantial increase in reactive oxygen species, and causes oxidative stress, resulting in toxic effects. Therefore, the method has important significance for human health by discharging the microplastic in the body and reducing the microplastic content.
There is no report on a method capable of removing microplastic in a human body. Methods for reducing contamination of plastics in the environment and water using biological methods have been reported, for example, some bacteria and fungi have been reported to have the ability to degrade plastics by secreting cutinases, proteases, esterases, lipases, etc., to break down polymers into monomers or oligomers. In addition, there are bacteria with the ability to capture and adsorb the microplastic, which can adhere to the surface of the microplastic and create a viscous biofilm, and this viscous matrix can capture free microplastic, causing the microplastic microorganisms to aggregate, thereby achieving separation and removal of the microplastic. However, bacteria and fungi capable of degrading or adsorbing the micro plastics are not edible microorganisms, and bacteria adsorbing the micro plastics in environments such as water generally need to form a biological film adsorbing the micro plastics for a long time, and meanwhile, the bacteria are difficult to tolerate the gastrointestinal tract environment. Therefore, the above method is difficult to be used for human microplastic removal.
Disclosure of Invention
The invention provides lactobacillus plantarum DT88 and application thereof in preparing products for adsorbing and removing microplastic.
The invention provides lactobacillus plantarum (Lactiplantibacillus plantarum) DT88, which is deposited with the microorganism strain collection (Guangdong Microbial Culture Collection Center, GDMCC, address: building 5, post code: 510070, no. 59, mitsui 100, guangzhou City, guangdong) of Guangdong province on day 5 of 2023, and is classified and named Lactiplantibacillus plantarum, with the deposit number of GDMCC No:63626.
in the present invention, lactobacillus plantarum DT88 strain was isolated from fish tea, and identified by bacterial morphology, physiology and 16S rRNA sequencing, and as a result, lactobacillus plantarum (Lactiplantibacillus plantarum) was named lactobacillus plantarum DT88.
Lactobacillus plantarum DT88 has the following microbiological characteristics:
(1) Morphological features
Gram staining is positive, cells are rod-shaped under a light microscope, two ends are round, and single cells or the cells are arranged in pairs and chains.
After 24h of culture in MRS solid medium, milky white colonies with round, convex, neat and smooth edges and moist surfaces are formed.
(2) Physiological characteristics
Lactobacillus plantarum DT88 can be grown in acidic or bile salt-containing media. The strain can effectively adsorb the microplastic, and the adsorption rate reaches 79%. Lactobacillus plantarum DT88 has an antioxidation effect, and can reduce oxidative damage caused by microplastic.
Lactobacillus plantarum DT88 can be cultivated by the following cultivation method: lactobacillus plantarum DT88 was inoculated into MRS broth and cultured anaerobically at 37℃for 24h. Wherein, the MRS broth culture medium comprises the following components: 10.0g/L of casein enzyme digests, 10.0g/L of beef powder, 4.0g/L of yeast powder, 2.0g/L of citric acid triammonium, 5.0g/L of sodium acetate, 0.2g/L of magnesium sulfate, 0.05g/L of manganese sulfate, 20.0g/L of glucose, 2.0g/L of dipotassium hydrogen phosphate, 1.0g/L of tween 80 and pH 5.7+/-0.2, and 1.5% of agar is added into the solid culture medium.
Lactobacillus plantarum is widely present in fermented foods and is commonly used in the food industry as a starter or preservative. Lactobacillus plantarum has been listed in a list of species useful for food, and in the list of European Union microbial species safety Qualification (QPS) issued by the European Union food Security agency (EFSA), GRAS (generally recognised as safe) certification by the United states Food and Drug Administration (FDA) was also obtained, and genome research data, mouse experiments and human clinical experiments have demonstrated the safety of lactobacillus plantarum in a wide variety of probiotic foods and dietary supplements worldwide.
The present invention provides a microbial preparation comprising lactobacillus plantarum DT88 as described above.
Preferably, in the microbial preparation described above, lactobacillus plantarum DT88 is present in the form of a living bacterium.
The microbial preparation described above may be a solid preparation (e.g., a bacterial powder) or a liquid preparation.
The invention provides a preparation method of the microbial preparation, which comprises the step of culturing the lactobacillus plantarum DT88.
Preferably, the culture is anaerobic at 35-37 ℃.
Preferably, the culturing is performed using MRS broth. After the culture is finished, bacterial liquid is collected and further prepared into a microbial preparation.
Based on the function of lactobacillus plantarum DT88, the present invention provides any of the following uses of the strain.
The present invention provides the use of said lactobacillus plantarum DT88 or said microbial preparation for the preparation of a product for adsorbing and/or facilitating the discharge of microplastic.
The lactobacillus plantarum DT88 has better acid resistance and cholate resistance, can well resist the gastrointestinal tract environment in vivo, and further plays roles of adsorbing microplastic in vivo, promoting the discharge of the microplastic and resisting oxidation.
In the above applications, the product is preferably a food, pharmaceutical or feed. The food is preferably a health food.
The invention provides the application of lactobacillus plantarum DT88 or the microbial preparation in adsorbing and/or removing micro-plastics in the environment.
The lactobacillus plantarum DT88 can also play the roles of adsorbing microplastic and promoting the clearance of the microplastic in an in-vitro environment, and can be used for adsorbing and/or clearing the microplastic in the environment, such as water, soil and the like.
The invention provides application of lactobacillus plantarum DT88 or the microbial preparation in preparing antioxidant products.
The lactobacillus plantarum DT88 has free radical scavenging capability, can play an antioxidant function, and reduces oxidative damage caused by microplastic. The lactobacillus plantarum DT88 has the functions of adsorbing the microplastic and resisting oxidization, so that the discharge of the microplastic in the body can be promoted, the oxidative damage caused by the residual microplastic in the body can be reduced, and the adverse effect on the body caused by the accumulation of the microplastic can be effectively reduced from the two aspects.
In the above applications, the product is preferably a food, pharmaceutical or feed. The food is preferably a dietary supplement or a health food.
The invention provides application of lactobacillus plantarum DT88 or the microbial preparation in preparing foods, medicines or feeds.
The present invention provides a food product comprising lactobacillus plantarum DT88 as described above or the microbial preparation.
The present invention provides a pharmaceutical product comprising lactobacillus plantarum DT88 as described above or the microbial preparation.
The invention provides a feed comprising lactobacillus plantarum DT88 as described above or the microbial preparation.
The medicines, foods and feeds can contain raw materials or auxiliary materials allowed in the fields of medicines, foods and feeds besides the lactobacillus plantarum DT88 or the microbial preparation. Wherein, the pharmaceutically acceptable auxiliary materials comprise filler, excipient, lubricant, wetting agent, diluent, etc. The type of preparation of the medicine may be a solid preparation (e.g., powder, granule, capsule, tablet, etc.) or a liquid preparation (e.g., oral liquid, etc.).
The invention provides a micro plastic adsorbent comprising lactobacillus plantarum DT88 as described above or the microbial preparation.
Compared with the prior art, the technical scheme provided by the invention has the beneficial effects that at least: the lactobacillus plantarum DT88 has better acid resistance and bile salt resistance, can resist the gastrointestinal tract environment, and has potential as edible probiotics; the strain can efficiently adsorb the microplastic, accelerate the discharge and the removal of the microplastic, has antioxidation capability, can reduce the oxidation damage caused by the accumulation of the microplastic, is hopeful to be developed into an edible probiotic product, and has the effects of adsorbing the microplastic, accelerating the discharge of the microplastic, reducing the damage of the microplastic and protecting the health of the gastrointestinal tract as intestinal bacteria in a human body.
Drawings
In order to more clearly illustrate the invention or the technical solutions of the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are some embodiments of the invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a colony morphology of Lactobacillus plantarum DT88 on MRS medium in example 1.
FIG. 2 is a microscopic image of Lactobacillus plantarum DT88 of example 1.
FIG. 3 shows the result of the survival rate of Lactobacillus plantarum DT88 in acidic medium in example 2, wherein ns represents p-values greater than 0.05 (data from three replicates, error bars represent standard deviations), and the statistical analysis method is t test.
FIG. 4 shows the result of the viability of Lactobacillus plantarum DT88 in bile salt-containing media in example 3, where ns represents p-values greater than 0.05 (data from three replicates, error bars represent standard deviations), and the statistical analysis method is t test.
FIG. 5 shows the result of adsorption and aggregation of Lactobacillus plantarum DT88 with PS fluorescent microspheres in solution in example 4.
Fig. 6 shows the adsorption rate of lactobacillus plantarum DT88 to PS fluorescent microspheres in solution in example 4, wherein p-value is less than 0.0001 (data from three replicates, error bars standard deviation), and statistical analysis method is t test.
FIG. 7 is an electron micrograph of Lactobacillus plantarum DT88 and PS fluorescent microsphere adsorbed in example 4 at 50000 Xmagnification.
FIG. 8 shows the clearance results of Lactobacillus plantarum DT88 on DPPH in example 5, wherein the data is from three replicates and the error bars represent standard deviations.
Control in fig. 3, 4, and 6 represents a Control.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
EXAMPLE 1 isolation and characterization of Lactobacillus plantarum DT88
1. Isolation and identification of Lactobacillus plantarum DT88
1.1 sample Source
Lactobacillus plantarum DT88 of the present invention is isolated from fish tea.
1.2 preparation of Medium
The culture medium used for sample separation is BBL culture medium, and Lactobacillus plantarum DT88 is cultured by MRS culture medium.
BBL medium components are shown in Table 1, MRS medium components are shown in Table 2, and solid medium is obtained by adding 1.5% agar.
TABLE 1BBL Medium formulation
TABLE 2MRS Medium formulation
1.3 isolation of strains
Placing 1g of fish tea sample into 10mL of BBL liquid culture medium prepared in step 1.2, uniformly mixing, culturing at 36 ℃ for 24h, sucking 1mL of enrichment liquid in an ultra-clean bench, performing ten-fold gradient dilution, and selecting 10 -4 、10 -5 、10 -6 、10 -7 Four bacterial solutions with dilution gradient of 100 mu L are coated on a culture dish containing sterile BBL solid culture medium, and are subjected to static culture at 36 ℃ for 48-72 hours under anaerobic condition until obvious single colonies are formed, a high-throughput automatic platform is used for automatically picking typical bacterial colonies from the culture dish to the BBL liquid culture medium for culture, and the isolated bacterial strains are subjected to 16S rRNA sequencing to determine species information.
2. Identification of Lactobacillus plantarum DT88
2.1 colony characterization
After culturing lactobacillus plantarum DT88 in MRS solid medium for 24h, it forms milky white colony with round shape, convex shape, neat and smooth edge and wet surface, see FIG. 1.
2.2 morphology under microscope
Lactobacillus plantarum DT88 colony smear: gram staining is positive, cells are rod-shaped under a light microscope, two ends are round, and single cells or the cells are arranged in pairs and chains. See fig. 2.
2.3 16S rRNA identification
Identification unit: optimum Trinitum Prinsepiae SpA.
Identification sequence: as shown in SEQ ID NO. 1.
Identification result: the sequencing result is compared with NCBI database, and the comparison result is combined with physiological and biochemical result to determine that the strain is lactobacillus plantarum (Lactiplantibacillus plantarum).
Lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 was deposited at 7.5.2023 with the Guangdong province microorganism strain collection center (Guangdong Microbial Culture Collection Center, GDMCC, address: building 5, 30 of the Guangzhou City, first, hirudo 100, guangdong, post code: 510070), classified as Lactiplantibacillus plantarum, accession number GDMCC No:63626.
example 2 acid resistance test of Lactobacillus plantarum DT88
The overall pH condition in the gastric environment of the human body is strongly acidic, so the acid resistance of the strain is an important index for evaluating whether the strain can survive and colonize in the gastric acid environment. The commercial strain lactobacillus rhamnosus Lactobacillus rhamnosus GG is a widely used probiotic and has strong acid resistance.
In this example, the acid resistance of lactobacillus plantarum DT88 was verified using MRS medium at ph=2.5. 1mL of the bacterial liquid was centrifuged at 4000rpm for 10min, the supernatant was discarded, and then 1mL of PBS was added for washing once, and after centrifugation at 4000rpm for 10min, the pellet was resuspended in MRS medium at pH=2.5. Incubate for 3h at 37℃and sample at 0h and 3h, respectively. After centrifugation, the samples were resuspended in PBS and diluted in a gradient, and the diluted samples were plated on MRS agar plates and subjected to colony counting after anaerobic incubation at 37℃for 16 h. The survival rate calculation formula is: acid-resistant survival (%) =c1/c0×100% (c0:0 h count; c1:3h count). The control strain is lactobacillus rhamnosus GG.
After 3 hours of incubation with acid medium, the control strain lactobacillus rhamnosus GG had a survival rate of 84.56% and lactobacillus plantarum DT88 had a survival rate of 62.53% (fig. 3), which was not significantly different from the control strain and was able to survive in the stomach environment.
EXAMPLE 3 detection of bile salt tolerance of Lactobacillus plantarum DT88
After entering the intestine through the stomach, the bacteria are killed by the high concentration of bile salts in the small intestine. The residence time of the food in the small intestine is generally 1 to 4 hours.
Thus, this example uses 0.1% bile salt-MRS medium to verify the bile salt tolerance of Lactobacillus plantarum DT88 strain. Lactobacillus plantarum DT88 bacterial liquid is inoculated in a 96 deep well plate containing MRS culture medium, and is subjected to anaerobic culture at 37 ℃ for 24 hours. 300. Mu.L of the cultured bacterial liquid was centrifuged at 4000rpm for 10min, the supernatant was discarded, 600. Mu.L of MRS medium containing 0.1% bile salt was added, and the mixture was resuspended. The control group was taken 100. Mu.L of the resuspension, and 20. Mu.L of MTT (thiazole blue) solution was added; the treatment group was taken as 100. Mu.L of the heavy suspension, and after incubation at 37℃for 4 hours, 20. Mu.L of MTT solution was added. After adding MTT solution, the reaction was carried out at 37℃for 4 hours in the absence of light, and after the completion of the reaction, the reaction was centrifuged at 4000rpm for 10 minutes, and the supernatant was discarded. 100 mu L of DMSO solution is added into each hole, and the mixture is incubated for 10min under shaking at 37 ℃ to completely dissolve and mix the blue-violet formazan generated by the reaction. After mixing, absorbance at 570nm was measured by using an enzyme-labeled instrument, and survival rate was calculated. Survival = A1/a0×100% (A1: absorbance at 570nm for treatment group solutions, A0: absorbance at 570nm for control group solutions). The viability of the control strain lactobacillus rhamnosus GG was determined in the same way.
After 4 hours of incubation with 0.1% bile salt-MRS medium, the survival rate of the control strain Lactobacillus rhamnosus GG was 101.5% and the survival rate of Lactobacillus plantarum DT88 was 100.9% (FIG. 4). This indicates that lactobacillus plantarum DT88 is comparable to the control strain lactobacillus rhamnosus GG in terms of bile salt tolerance and is able to survive in the small intestine.
Example 4 measurement of the Effect of Lactobacillus plantarum DT88 on adsorbing microplastic
Lactobacillus plantarum DT88 was inoculated into MRS medium and cultured anaerobically at 37℃for 24h. The cultured bacterial solution was centrifuged at 4000rpm for 10min, the supernatant was discarded, and 450. Mu.L of sterile PBS buffer was added for washing 2 times. Then PBS is added for resuspension, and the concentration of bacterial liquid is adjusted to be 1 multiplied by 10 9 CFU/mL. The experimental group takes 100 mu L to 1.5mL EP tube of lactobacillus plantarum DT88 bacterial suspension, 900 mu L PS fluorescent microsphere working solution (0.16 mg/mL, particle size 0.1 mu m, sile company) is added and mixed evenly, and the mixture is placed in a shaking table to shake and incubate for 4 hours under the conditions of 37 ℃ and 800rpm. The blank group was incubated with 100. Mu.L PBS and 900. Mu.L PS fluorescent microsphere working solution in a 1.5mL EP tube. The bacterial liquid control group is prepared by uniformly mixing and incubating 100 mu L of lactobacillus plantarum DT88 bacterial suspension and 900 mu L of PBS into a 1.5mL EP tube. After the incubation is finished, taking the incubation liquid for observation and photographing, and obtaining the resultFig. 5.
Taking the incubation liquid of the experimental group and the blank control group, centrifuging at 2000rpm for 10min, taking 100 mu L of supernatant, and measuring the fluorescence intensity by using an enzyme-labeling instrument. The parameters of the enzyme label instrument are as follows: excitation wavelength: 494nm; detection wavelength: 518nm. And calculating the adsorption rate according to the fluorescence intensity value. The control strain was another lactobacillus plantarum selected in the same batch of screening experiments, and the adsorption rate of the control strain was measured in the same manner, and the result is shown in fig. 6. The adsorption rate calculation formula is: adsorption (%) = (A1-A2)/a1×100% (A1: fluorescence value of the blank group, A2: fluorescence value of the lactobacillus plantarum group). Taking the precipitate after centrifugation, fixing the precipitate at 4 ℃ by glutaraldehyde overnight, carrying out gradient dehydration by ethanol, drying, and observing the precipitate by an electron microscope, wherein the result is shown in figure 7.
As can be seen from fig. 5, PS fluorescent microspheres did not self-aggregate in the blank group; in the bacteria liquid control group, the lactobacillus plantarum DT88 does not self-coagulate; in the experimental group, lactobacillus plantarum DT88 and PS fluorescent microspheres show specific adsorption and flocculation.
As can be seen from FIG. 6, the adsorption rate of the control strain is 8.03%, the adsorption capacity of the microplastic is poor, while the adsorption rate of the Lactobacillus plantarum DT88 reaches 79.78%, and the microplastic adsorption capacity is very strong. This indicates that lactobacillus plantarum DT88 has strain specificity for the adsorption effect of microplastic.
As can be seen from fig. 7, under observation by an electron microscope, spherical PS fluorescent microspheres were adsorbed on the surface of lactobacillus plantarum DT88.
EXAMPLE 5 antioxidant Capacity determination of Lactobacillus plantarum DT88
Lactobacillus plantarum DT88 was inoculated into MRS medium and cultured anaerobically at 37℃for 24h. Centrifuging the cultured bacterial liquid at 4000rpm for 10min, discarding supernatant, adding 450 μl of sterile PBS buffer, washing for 2 times, and adjusting bacterial liquid concentration to 1×10 9 CFU/mL. Experimental groups 500. Mu.L of the bacterial suspension were taken and 500. Mu.L of 0.2 mmoL/L1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) ethanol solution was added. The antioxidant vitamin C (Vc) was used as a positive control and 500. Mu.L of 3. Mu.g/mL vitamin solution was added to 500. Mu.L of 0.2mmoL/L DPPH ethanol solution. The control group was taken from 500. Mu.L of PBS and 500. Mu.L of 0.2mmoL/L DPPH ethanol solution was added. Empty spaceThe white group was prepared by adding 500. Mu.L of the bacterial suspension to 500. Mu.L of an absolute ethanol solution. Mixing, and shaking in a shaking table at 30deg.C for 30min. After the completion of the shaking, the reaction mixture was centrifuged at 4000rpm for 10 minutes, and 100. Mu.L of the supernatant was subjected to absorbance measurement at 517nm by using a microplate reader. DPPH radical scavenging was calculated. The calculation formula is as follows: DPPH clearance (%) = [1- (As-A0)/Ai]X 100% (As: experimental; A0: blank; ai: control).
As can be seen from fig. 8, the antioxidant vitamin C has a clearance rate of 60.90% for DPPH, and similar to vitamin C, lactobacillus plantarum DT88 has a certain antioxidant capacity, and a clearance rate of 35.36% for DPPH. Thus, colonization of Lactobacillus plantarum DT88 may reduce the oxidative damage of the host by the microplastic.
In conclusion, the lactobacillus plantarum DT88 is obtained through separation and screening. The strain is acid-resistant and cholate-resistant, has the capability of field planting in stomach and small intestine, and can be applied to development of edible probiotics. The lactobacillus plantarum DT88 has strong capacity of adsorbing microplastic, and experimental data also prove that the strain has antioxidant capacity. Therefore, the lactobacillus plantarum DT88 is a strain suitable for the digestive tract environment, and has wide application prospect in the aspects of adsorbing microplastic, accelerating the discharge of the microplastic and reducing the oxidative damage caused by the microplastic.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A lactobacillus plantarum (Lactiplantibacillus plantarum) DT88, wherein the lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 is deposited with the cantonese province microorganism strain collection under the accession number GDMCC No:63626.
2. a microbial formulation comprising lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 as claimed in claim 1.
3. A method of preparing a microbial preparation according to claim 2, characterized in that the method comprises the step of culturing the lactobacillus plantarum (Lactiplantibacillus plantarum) DT88.
4. Use of lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 according to claim 1 or a microbial preparation according to claim 2 for the preparation of a product for adsorbing and/or facilitating the discharge of microplastic.
5. Use of lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 as claimed in claim 1 or a microbial preparation as claimed in claim 2 for adsorbing and/or scavenging micro-plastics in an environment.
6. Use of lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 as claimed in claim 1 or a microbial preparation as claimed in claim 2 for the manufacture of an antioxidant product.
7. Use of lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 as claimed in claim 1 or a microbial preparation as claimed in claim 2 in the preparation of a food, pharmaceutical or feed product.
8. A food product, characterized in that it comprises lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 according to claim 1 or a microbial preparation according to claim 2.
9. A pharmaceutical product comprising lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 according to claim 1 or a microbial preparation according to claim 2.
10. A microplastic adsorbent comprising lactobacillus plantarum (Lactiplantibacillus plantarum) DT88 according to claim 1 or a microbial preparation according to claim 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310941575.2A CN117089488A (en) | 2023-07-28 | 2023-07-28 | Lactobacillus plantarum DT88 and application thereof in preparation of micro-plastic adsorption and removal products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310941575.2A CN117089488A (en) | 2023-07-28 | 2023-07-28 | Lactobacillus plantarum DT88 and application thereof in preparation of micro-plastic adsorption and removal products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117089488A true CN117089488A (en) | 2023-11-21 |
Family
ID=88776188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310941575.2A Pending CN117089488A (en) | 2023-07-28 | 2023-07-28 | Lactobacillus plantarum DT88 and application thereof in preparation of micro-plastic adsorption and removal products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117089488A (en) |
-
2023
- 2023-07-28 CN CN202310941575.2A patent/CN117089488A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112358999B (en) | Lactobacillus reuteri and application thereof | |
| CN112080445A (en) | Lactobacillus plantarum ZJ316 and application thereof in inhibiting helicobacter pylori | |
| AU2020103929A4 (en) | Bacillus coagulans strain BACO-17 with high germination rate in the intestines and its uses for promoting gastrointestinal health | |
| CN115044504B (en) | Enterococcus faecalis YZ-1 and probiotic application thereof | |
| CN111534435B (en) | Freeze-drying protective agent capable of improving acid resistance of bifidobacteria and application thereof | |
| CN114085789A (en) | Pediococcus pentosaceus MA.WTPQJ01 and application thereof | |
| CN116970512A (en) | Lactobacillus plantarum, and culture method and application thereof | |
| CN116421632A (en) | Application of pediococcus acidilactici in preparing food, health-care product or medicine with lipid-lowering effect | |
| CN117089487A (en) | Lactobacillus paracasei DT33 and application thereof in preparation of products with functions of micro-plastic adsorption, removal and defecation promotion | |
| CN117187113A (en) | Lactobacillus paracasei DT11 and application thereof in preparation of products with functions of micro-plastic adsorption, removal and defecation promotion | |
| CN112877231B (en) | Lactic acid bacteria with antibacterial and antioxidant activities and application thereof | |
| CN113088468A (en) | Lactobacillus casei Ma. GLRGJ1 and application thereof | |
| CN115466693B (en) | Stokes Bei Jisi Rumex bacillus CY2 strain and application thereof | |
| CN117089488A (en) | Lactobacillus plantarum DT88 and application thereof in preparation of micro-plastic adsorption and removal products | |
| CN113913334B (en) | Enterococcus faecalis EF-ZA1107-06 and application thereof | |
| CN117683674A (en) | Lactobacillus fermentum 755 and application thereof | |
| CN117327616A (en) | Lactiplantibacillus plantarum ZN1 and application thereof | |
| CN111944729B (en) | High-temperature-resistant lactobacillus plantarum microbial inoculum and preparation method and application thereof | |
| CN117187111A (en) | Lactobacillus plantarum DT77 and application thereof in preparation of products with micro-plastic adsorption and removal functions | |
| CN116987645A (en) | Lactobacillus plantarum (Lactiplantibacillus plantarum) HEPRO-185 and application thereof | |
| CN113604387B (en) | Salt-tolerant and high-temperature-resistant lactobacillus reuteri and application thereof in prevention and treatment of pathogenic bacteria in livestock and poultry aquaculture | |
| CN117089486A (en) | Lactobacillus plantarum DT55 and application thereof in preparation of products with micro-plastic adsorption and removal functions | |
| CN117187112A (en) | Lactobacillus paracasei DT66 and application thereof in preparation of products with micro-plastic adsorption and removal functions | |
| CN116286480A (en) | Screening and separating method for clostridium butyricum and application thereof | |
| CN114107127B (en) | Bacillus amyloliquefaciens D1 capable of degrading lipopolysaccharide and producing protease at high yield and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20231121 |