CN117070345A - Temperature control equipment and method for biological reaction container - Google Patents

Abstract

The invention provides temperature control equipment and a temperature control method for a biological reaction container, wherein the temperature control equipment for the biological reaction container comprises a first TCU, the biological reaction container and a liquid storage tank, wherein a first liquid inlet end, a first liquid outlet end and a first liquid return end are arranged on the first TCU, a refrigeration liquid inlet end, a refrigeration liquid outlet end and a refrigeration liquid return end are arranged on the liquid storage tank, the first liquid outlet end is communicated with the refrigeration liquid inlet end through a first liquid pump, the first liquid inlet end is communicated with a liquid inlet pipe of the equipment through a first electromagnetic valve, the first liquid outlet end is communicated with the liquid outlet pipe of the equipment through a second electromagnetic valve, the refrigeration liquid outlet end is communicated with the biological reaction container through a second liquid pump, and the refrigeration liquid return end is communicated with the biological reaction container through a fourth electromagnetic valve. Through setting up the first TCU that has the refrigeration function, the cooperation stores the liquid case, refrigerates the liquid that gets into first TCU, forms the refrigerating fluid, and the refrigerating fluid carries out the heat exchange in the inside of biological reaction container, makes biological reaction container's internal environment just cool down by a wide margin fast.

Description

Temperature control equipment and method for biological reaction container

Technical Field

The invention relates to the technical field of pharmaceutical equipment, in particular to temperature control equipment and a temperature control method for a biological reaction container.

Background

The biological reaction vessel is a vessel for providing a reaction environment for biological reaction in the biological pharmacy process. The biological reaction carried out in the container requires a sterile environment and also requires the temperature of the environment, which is to be raised and lowered in a short time. Generally, the process of heating up or cooling down needs to be completed within ten minutes.

Currently, there are two conventional approaches: firstly, a jacket is arranged outside the biological reaction container, a runner is arranged in the jacket and communicated with a TCU (Temperature Control Unit ), and the temperature of reactants is controlled by controlling the temperature of fluid in the runner; second, a coil is placed in the bioreactor vessel and is connected to the TCU, and the temperature of the reactants is controlled by controlling the temperature of the fluid in the coil. The temperature control speed of the coil is faster than that of the jacket.

In the prior art, TCU has limited power and slow temperature control speed, especially the temperature rise in the reaction vessel by adopting a jacket temperature control mode can only marginally meet the requirement of short-time temperature rise, and the speed of cooling in the reaction vessel by adopting two temperature control modes is slower than the required speed. In addition, the coil pipe arranged in the reaction vessel needs to be fixed, and the coil pipe is generally made of a metal pipe, so that the material is not safe, and the coil pipe cannot be completely prevented from participating in biological reaction.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide temperature control equipment and a temperature control method for a biological reaction container, and aims to solve the problem that the temperature control equipment in the prior art is difficult to quickly refrigerate the inside of the biological reaction container.

In order to achieve the above object, the present invention is achieved by the following technical scheme:

the utility model provides a temperature control equipment of biological reaction container, includes first TCU, biological reaction container and stock solution case, first TCU is used for the liquid refrigeration to getting into it, set up first feed liquor end, first flowing back end, first play liquid end and first back liquid end on the first TCU, set up refrigeration feed liquor end, refrigeration flowing back end, refrigeration play liquid end and refrigeration back liquid end on the stock solution case, first play liquid end passes through first liquid pump intercommunication refrigeration feed liquor end, so that first TCU intercommunication stock solution case, the stock solution case is used for storing the liquid after the refrigeration, first back liquid end intercommunication refrigeration flowing back end, first feed liquor end passes through first solenoid valve intercommunication equipment feed liquor pipe, so that liquid is from equipment feed liquor pipe gets into first TCU, first flowing back end passes through second solenoid valve intercommunication equipment feed liquor pipe, so that liquid is from first TCU passes through equipment drain liquor to outside, second liquid pump passes through second liquid pump intercommunication biological reaction liquid after the stock solution case is used for storing the liquid, first liquid return liquid pump passes through the second solenoid valve intercommunication biological reaction container carries out the biological reaction container.

Compared with the prior art, the invention has the beneficial effects that: through setting up have refrigeration function first TCU, the cooperation the stock solution case is cooled down the liquid that gets into first TCU forms the refrigerating fluid, the refrigerating fluid is in the inside of biological reaction container carries out the heat exchange, makes biological reaction container's internal environment just cools down by a wide margin fast, reaches the required low temperature of reactant in the biological reaction container.

Further, the temperature control equipment of the biological reaction container further comprises a second TCU, the second TCU is used for performing temperature control treatment on liquid entering the second TCU, a second liquid inlet end, a second liquid outlet end, a second liquid return end and a second liquid return end are arranged on the second TCU, the second liquid inlet end is communicated with the equipment liquid inlet pipe through a fifth electromagnetic valve so that the liquid enters the second TCU from the equipment liquid inlet pipe, the second liquid outlet end is communicated with the equipment liquid outlet pipe through a sixth electromagnetic valve so that the liquid is discharged to the outside from the second TCU through the equipment liquid outlet pipe, the second liquid outlet end is communicated with the biological reaction container through a third liquid pump, a seventh electromagnetic valve is arranged between the third liquid pump and the biological reaction container, and the second liquid return end is communicated with the biological reaction container through an eighth electromagnetic valve.

Still further, set up ninth solenoid valve between third liquid pump with equipment feed liquor pipe, the second goes out the liquid end through third liquid pump, ninth solenoid valve and first solenoid valve intercommunication first feed liquor end.

Still further, equipment feed liquor pipe intercommunication outside, just set up pressure valve on the equipment feed liquor pipe is close to the one end of outside, pressure valve is used for adjusting the pressure when liquid gets into the control by temperature change equipment of biological reaction container.

Still further, set up container feed liquor pipe and container fluid-discharge tube on the biological reaction container, set up the hose in the biological reaction container, the hose is used for holding the liquid of specific temperature, so that the biological reaction container internal environment intensifies or drops the temperature, container feed liquor pipe the container fluid-discharge tube reaches the hose all communicates each other, seventh solenoid valve passes through container feed liquor pipe intercommunication the hose, just the seventh solenoid valve with set up first check valve between the container feed liquor pipe, third solenoid valve passes through container feed liquor pipe intercommunication the hose, just the third solenoid valve with set up the second check valve between the container feed liquor pipe, fourth solenoid valve with eighth solenoid valve all passes through container fluid-discharge tube intercommunication the hose, container fluid-discharge tube passes through tenth solenoid valve intercommunication equipment fluid-discharge tube.

Still further, the hose is the kink shape, just the kink of hose butt biological reaction vessel's inside wall, set up metal support piece in the lateral wall of hose, metal support piece is located the kink of hose.

Still further, the hose is disposed around an inner sidewall of the bioreactor vessel.

The embodiment of the invention also provides a temperature control method of the biological reaction container, which is applied to the temperature control equipment of the biological reaction container in the technical scheme, and comprises the following steps:

s1, enabling liquid to enter a liquid inlet pipe of the equipment, opening the first electromagnetic valve, keeping the fifth electromagnetic valve closed, enabling the liquid to enter the first TCU through the first electromagnetic valve and the first liquid inlet end, and refrigerating the liquid by the first TCU to form refrigerating liquid;

s2, starting the first liquid pump, enabling the refrigerating fluid to enter the storage fluid tank from the first TCU through the first fluid outlet end, the first liquid pump and the refrigerating fluid inlet end for storage, and measuring the temperature of the refrigerating fluid in the storage fluid tank to obtain a detection temperature;

s3, after the detected temperature reaches the reaction temperature required in the biological reaction container, the first liquid pump stops working, the second liquid pump and the third electromagnetic valve are started, and refrigerating fluid enters the hose from the liquid storage tank through the refrigerating liquid outlet end, the second liquid pump, the third electromagnetic valve and the container liquid inlet pipe to cool reactants in the biological reaction container;

s4, opening the fourth electromagnetic valve, and enabling the refrigerating fluid to form cold return fluid after heat exchange with reactants in the biological reaction container, wherein the cold return fluid is discharged from the container liquid discharge pipe and enters the storage fluid tank through the fourth electromagnetic valve and the refrigerating return fluid end to form circulation.

Drawings

FIG. 1 is a schematic structural view of a temperature control apparatus for a bioreactor container according to a first embodiment of the present invention;

FIG. 2 is a schematic view showing the arrangement of hoses in a temperature control apparatus for a bioreactor container according to a first embodiment of the present invention;

FIG. 3 is a schematic view showing the arrangement of hoses in a temperature control apparatus of a bioreactor container according to a second embodiment of the present invention;

FIG. 4 is a flow chart of a method for controlling the temperature of a bioreactor container according to a third embodiment of the present invention;

description of main reference numerals:

first TCU	10	Second TCU	20
				First electromagnetic valve	31	Second electromagnetic valve	32
Third electromagnetic valve	33	Fourth electromagnetic valve	34
				Fifth electromagnetic valve	35	Sixth electromagnetic valve	36
Seventh electromagnetic valve	37	Eighth electromagnetic valve	38
				Ninth electromagnetic valve	39	First liquid pump	41
Second liquid pump	42	Third liquid pump	43
				Storage liquid tank	50	Liquid inlet pipe of equipment	61
Pressure valve	62	Liquid discharge pipe of equipment	63
				Tenth electromagnetic valve	64	First check valve	65
Second check valve	66	Biological reaction vessel	70
				Flexible pipe	71	Metal support	72

The invention will be further described in the following detailed description in conjunction with the above-described figures.

Detailed Description

In order that the invention may be readily understood, a more complete description of the invention will be rendered by reference to the appended drawings. Several embodiments of the invention are presented in the figures. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1, a temperature control device of a bioreactor in a first embodiment of the present invention includes a first TCU10, a second TCU20, a bioreactor 70 and a storage tank 50, wherein the first TCU10 is configured to refrigerate a liquid entering the first TCU10, a first liquid inlet end, a first liquid outlet end and a first liquid return end are disposed on the first TCU10, a refrigeration liquid inlet end, a refrigeration liquid outlet end and a refrigeration liquid return end are disposed on the storage tank 50, the first liquid outlet end is communicated with the refrigeration liquid inlet end through a first liquid pump 41, so that the first TCU10 is communicated with the storage tank 50, the storage tank 50 is configured to store the refrigerated liquid, the first liquid return end is communicated with the refrigeration liquid outlet end, the first liquid inlet end is communicated with a device liquid inlet pipe 61 through a first solenoid valve 31, so that the liquid enters the first TCU10 from the device liquid inlet pipe 61, the first liquid draining end is communicated with a device liquid draining pipe 63 through a second electromagnetic valve 32 so that the liquid is drained to the outside from the first TCU10 through the device liquid draining pipe 63, the refrigerating liquid draining end is communicated with the biological reaction container 70 through a second liquid pump 42 so that the liquid enters the biological reaction container 70 to exchange heat, a third electromagnetic valve 33 is arranged between the second liquid pump 42 and the biological reaction container, the refrigerating liquid returning end is communicated with the biological reaction container through a fourth electromagnetic valve 34, the second TCU20 is used for performing temperature control treatment on the liquid entering the biological reaction container, a second liquid inlet end, a second liquid draining end, a second liquid returning end and a second liquid returning end are arranged on the second TCU20, the second liquid inlet end is communicated with the device liquid inlet pipe 61 through a fifth electromagnetic valve 35 so that the liquid enters the second TCU20 from the device liquid inlet pipe 61, the second liquid outlet end is communicated with the equipment liquid outlet pipe 63 through a sixth electromagnetic valve 36 so that the liquid is discharged from the second TCU20 to the outside through the equipment liquid outlet pipe 63, the second liquid outlet end is communicated with the biological reaction container 70 through a third liquid pump 43, a seventh electromagnetic valve 37 is arranged between the third liquid pump 43 and the biological reaction container 70, and the second liquid return end is communicated with the biological reaction container 70 through an eighth electromagnetic valve 38. Preferably, temperature measuring devices are disposed in the first TCU10, the second TCU20, and the storage tank 50, the temperature measuring devices are used for monitoring water temperature, the storage tank 50 has a heat preservation function, and the bioreactor 70 is a disposable bioreactor made of co-extrusion film.

The ninth electromagnetic valve 39 is arranged between the third liquid pump 43 and the equipment liquid inlet pipe 61, the second liquid outlet end is communicated with the first liquid inlet end through the third liquid pump 43, the ninth electromagnetic valve 39 and the first electromagnetic valve 31, the equipment liquid inlet pipe 61 is communicated with the outside, a pressure valve 62 is arranged at one end, close to the outside, of the equipment liquid inlet pipe 61, and the pressure valve 62 is used for adjusting the pressure when liquid enters the temperature control equipment of the biological reaction container. It will be appreciated that when the pressure valve 62 is opened, liquid may be introduced into the temperature control device of the bioreactor from the outside, and when the pressure valve 62 is closed, the device liquid inlet pipe 61 may be used as a part of an internal circuit of the temperature control device of the bioreactor, the liquid outlet of the second TCU20 may be pumped to the device liquid inlet pipe 61 through the third liquid pump 43 and the ninth electromagnetic valve 39 to prepare the first TCU10 for use, the first TCU10 and the second TCU20 may cooperate with refrigeration, i.e. second order refrigeration, or the temperature control mode of the second TCU20 may be switched to refrigeration after heating with the second TCU20 to cooperate with the first TCU10 according to the requirement of the reaction temperature.

Referring to fig. 1 and 2, a container liquid inlet pipe and a container liquid outlet pipe are disposed on the bioreactor container 70, a hose 71 is disposed in the bioreactor container 70, the hose 71 is used for containing a liquid with a specific temperature, so as to raise or lower the temperature of the environment in the bioreactor container 70, the container liquid inlet pipe, the container liquid outlet pipe and the hose 71 are all mutually communicated, the seventh solenoid valve 37 is communicated with the hose 71 through the container liquid inlet pipe, a first check valve 65 is disposed between the seventh solenoid valve 71 and the container liquid inlet pipe, the third solenoid valve 33 is communicated with the hose 71 through the container liquid inlet pipe, a second check valve 66 is disposed between the third solenoid valve 33 and the container liquid inlet pipe, the fourth solenoid valve 34 and the eighth solenoid valve 38 are both communicated with the hose through the container liquid outlet pipe, and the container liquid outlet pipe is communicated with the equipment liquid outlet pipe 63 through the tenth solenoid valve 64.

The hose 71 is in a bent shape, the bent part of the hose 71 abuts against the inner side wall of the bioreactor 70, a metal support 72 is arranged in the side wall of the hose 71, and the metal support 72 is located at the bent part of the hose 71. Preferably, the hose 71 is made of silica gel and coiled in the bioreactor container 70, specifically, the hose 71 is coiled in a reactant, the bioreactor container 70 may be in a cylindrical shape or a cubic shape, the metal support 72 is approximately in a spring shape, is buried in the inner wall of the hose 71 and is used for supporting the hose 71, so that the inner part of the hose 71 is hollow all the time, the inner blockage caused by bending is prevented, the smooth waterway is ensured, the normal temperature control efficiency is ensured, the support hardware is arranged outside the bioreactor container 70, a magnet is arranged on the support hardware, and the magnet adsorbs the metal support 72, so that the purpose of fixing the hose 71 is achieved, the number of circles and the positions of the hose 71 when coiled can be adjusted according to the height of the reactant in the bioreactor container 70, the fixed position of the hose 71 corresponds to the position of the reactant, and only the reactant is subjected to heat exchange, so that the temperature control efficiency is improved.

Referring to fig. 1 and 3, the temperature control apparatus for a bioreactor according to the second embodiment of the present invention is different in that the hose 71 of the temperature control apparatus for a bioreactor is disposed around the inner sidewall of the bioreactor 70. Preferably, the co-extruded film is welded to the inner wall of the co-extruded film of the bioreactor 70 to form a bent-coil channel, and the hoses 71 may be divided into several groups, and each group may be independently arranged on each inner wall of the bioreactor 70. In other embodiments of the present invention, the straight path portion of the flexible tube 71 may be bent after crossing all the inner side walls of the bioreactor container 70. It will be appreciated that the co-extruded film has a degree of toughness that prevents the flexible channels formed from clogging at the bends.

Referring to fig. 4, a temperature control method for a bioreactor according to a third embodiment of the present invention is applied to the temperature control apparatus for a bioreactor according to the above embodiment, and includes the following steps:

s1, enabling liquid to enter a liquid inlet pipe of the equipment, opening the first electromagnetic valve, keeping the fifth electromagnetic valve closed, enabling the liquid to enter the first TCU through the first electromagnetic valve and the first liquid inlet end, and refrigerating the liquid by the first TCU to form refrigerating liquid;

the liquid entering the liquid inlet pipe 61 of the apparatus may be a normal temperature liquid introduced from outside the temperature control apparatus of the bioreactor container, or may be a liquid subjected to the temperature control treatment of the second TCU20, and at this time, the fifth electromagnetic valve 35 is kept in a closed state so that the liquid smoothly enters the first TCU10.

S2, starting the first liquid pump, enabling the refrigerating fluid to enter the storage fluid tank from the first TCU through the first fluid outlet end, the first liquid pump and the refrigerating fluid inlet end for storage, and measuring the temperature of the refrigerating fluid in the storage fluid tank to obtain a detection temperature;

preferably, to ensure the refrigerating effect, if the detected temperature obtained in the storage tank 50 is higher than the temperature required by the reaction, the first liquid pump 41 is started to allow more refrigerating liquid to enter the storage tank 50, and the liquid in the storage tank 50 returns to the first TCU10 through the refrigerating liquid discharging end and the first liquid returning end to cool again, so as to form an internal circulation.

S3, after the detected temperature reaches the reaction temperature required in the biological reaction container, the first liquid pump stops working, the second liquid pump and the third electromagnetic valve are started, and refrigerating fluid enters the hose from the liquid storage tank through the refrigerating liquid outlet end, the second liquid pump, the third electromagnetic valve and the container liquid inlet pipe to cool reactants in the biological reaction container;

it will be appreciated that the first TCU10 and the storage tank 50 cooperate to perform refrigeration on the reactant in the bioreactor 70, after the first TCU10 pre-cools the liquid, the refrigerant is stored in the storage tank 50 with a heat preservation function, and the refrigerant in the storage tank 50 may participate in the internal circulation, so that the temperature required for the reaction can be stably maintained.

S4, opening the fourth electromagnetic valve, and enabling the refrigerating fluid to form cold return fluid after heat exchange with reactants in the biological reaction container, wherein the cold return fluid is discharged from the container liquid discharge pipe and enters the storage fluid tank through the fourth electromagnetic valve and the refrigerating return fluid end to form circulation.

A refrigerating cycle of liquid is formed between the storage tank 50 and the bioreactor 70, and the temperature of the reactant in the bioreactor 70 is continuously reduced to reach the required reaction temperature.

Preferably, before the step of making the liquid enter the liquid inlet pipe of the device, opening the first electromagnetic valve, and making the liquid enter the first TCU through the first electromagnetic valve and the first liquid inlet end, the first TCU refrigerates the liquid, and before the step of forming the refrigeration liquid, the method may further include the following steps:

opening and adjusting the pressure valve 62, opening the fifth electromagnetic valve 35, keeping the first electromagnetic valve 31 closed, and allowing liquid to enter the second TCU20 from the outside through the equipment liquid inlet pipe 61, the fifth electromagnetic valve 35 and the second liquid inlet end, wherein the second TCU20 pre-refrigerates the liquid to form pre-refrigerating liquid;

closing the pressure valve 62, and opening the third liquid pump 43 and the ninth electromagnetic valve 39, so that the prefabricated cold liquid enters the equipment liquid inlet pipe 61 from the second TCU20 through the second liquid outlet end, the third liquid pump 43 and the ninth electromagnetic valve 39, and the first TCU10 is used.

It will be appreciated that if the temperature selected by the operator is low and the temperature difference from the inside of the bioreactor vessel 70 is large, the second TCU20 may be used for pre-cooling, and the first TCU10 may be used for performing a second-order cooling method of re-cooling, so that the temperature inside the bioreactor vessel 70 is rapidly reduced to the desired temperature.

Preferably, after the opening and adjusting the pressure valve, the fifth electromagnetic valve is opened, the first electromagnetic valve is kept closed, so that the liquid enters the second TCU from the outside through the liquid inlet pipe of the device, the fifth electromagnetic valve and the second liquid inlet end, and the second TCU pre-refrigerates the liquid, and before the step of forming pre-refrigerating liquid, the method may further include the following steps:

if the inside of the bioreactor container 70 needs to be heated first, the pressure valve 62 is adjusted to enable liquid to enter the liquid inlet pipe 61 of the temperature control device of the bioreactor container from the outside, the fifth electromagnetic valve 35 is opened, the liquid enters the second TCU20 through the fifth electromagnetic valve 35 and the second liquid inlet end, and the second TCU20 heats the liquid to form heating liquid;

keeping the ninth electromagnetic valve 39 closed, opening the third liquid pump 43 and the seventh electromagnetic valve 37, and allowing heating liquid to enter the hose 71 from the second TCU20 through the third liquid pump 43, the seventh electromagnetic valve 37 and the container liquid inlet pipe to heat the reactant in the bioreactor container;

opening the eighth electromagnetic valve 38, and forming a hot return liquid after heat exchange with the reactant in the biological reaction container 70, wherein the hot return liquid is discharged from the container liquid discharge pipe and enters the second TCU20 through the eighth electromagnetic valve 38 and the second liquid return end to form a cycle;

after the heating of the inside of the bioreactor container 70 is completed, the tenth electromagnetic valve 64 is opened, and the hot return liquid is discharged from the temperature control device of the bioreactor container through the tenth electromagnetic valve 64 and the device drain pipe 63.

It can be understood that the hot liquid is directly discharged out of the temperature control equipment of the biological reaction container, the heated liquid is not required to be cooled, the cooling speed is improved, the temperature control efficiency is improved, and the temperature control equipment of the biological reaction container can finish the rapid switching of heating and refrigerating modes. Preferably, if separate heating of the bioreactor vessel 70 is desired, a thermal circulation of the liquid between the second TCU20 and the bioreactor vessel 70 is maintained after the hot return liquid returns to the second TCU 20.

Preferably, the liquid used as the temperature control medium in the temperature control device of the biological reaction vessel is warm water.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims (8)

1. The utility model provides a temperature control equipment of biological reaction container, its characterized in that includes first TCU, biological reaction container and stock solution case, first TCU is used for the liquid refrigeration that gets into it, set up first feed liquor end, first flowing back end, first play liquid end and first back liquid end on the first TCU, set up refrigeration feed liquor end, refrigeration flowing back end, refrigeration play liquid end and refrigeration back liquid end on the stock solution case, first play liquid end passes through first liquid pump intercommunication refrigeration feed liquor end, so that first TCU intercommunication stock solution case, the stock solution case is used for storing the liquid after the refrigeration, first back liquid end intercommunication refrigeration flowing back end, first feed liquor end passes through first solenoid valve intercommunication equipment feed liquor pipe, so that the liquid is from equipment feed liquor pipe gets into first TCU, first flowing back end passes through the second solenoid valve intercommunication equipment drain liquor pipe, so that the liquid is from first TCU passes through equipment to outside, the first liquid pump intercommunication biological reaction container passes through second liquid pump intercommunication biological reaction container passes through the second liquid pump intercommunication biological reaction container is carried out between the biological reaction container.

2. The temperature control device of a bioreactor according to claim 1, further comprising a second TCU, wherein the second TCU is configured to perform temperature control treatment on a liquid entering the second TCU, a second liquid inlet end, a second liquid outlet end, and a second liquid return end are disposed on the second TCU, the second liquid inlet end is connected to the device liquid inlet pipe through a fifth electromagnetic valve, so that the liquid enters the second TCU from the device liquid inlet pipe, the second liquid outlet end is connected to the device liquid outlet pipe through a sixth electromagnetic valve, so that the liquid is discharged from the second TCU to the outside through the device liquid outlet pipe, the second liquid outlet end is connected to the bioreactor through a third liquid pump, a seventh electromagnetic valve is disposed between the third liquid pump and the bioreactor, and the second liquid return end is connected to the bioreactor through an eighth electromagnetic valve.

3. The temperature control device of the biological reaction container according to claim 2, wherein a ninth electromagnetic valve is arranged between the third liquid pump and the liquid inlet pipe of the device, and the second liquid outlet end is communicated with the first liquid inlet end through the third liquid pump, the ninth electromagnetic valve and the first electromagnetic valve.

4. A temperature control device for a bioreactor vessel according to claim 3, wherein the device liquid inlet pipe is communicated with the outside, and a pressure valve is arranged at one end of the device liquid inlet pipe close to the outside, and the pressure valve is used for adjusting the pressure when liquid enters the temperature control device for the bioreactor vessel.

5. The apparatus according to claim 4, wherein a container liquid inlet pipe and a container liquid outlet pipe are provided on the bioreactor container, a hose is provided in the bioreactor container, the hose is used for containing liquid with a specific temperature so as to raise or lower the temperature of the environment in the bioreactor container, the container liquid inlet pipe, the container liquid outlet pipe and the hose are all mutually communicated, the seventh electromagnetic valve is communicated with the hose through the container liquid inlet pipe, a first check valve is provided between the seventh electromagnetic valve and the container liquid inlet pipe, the third electromagnetic valve is communicated with the hose through the container liquid inlet pipe, a second check valve is provided between the third electromagnetic valve and the container liquid inlet pipe, the fourth electromagnetic valve and the eighth electromagnetic valve are both communicated with the hose through the container liquid outlet pipe, and the container liquid outlet pipe is communicated with the apparatus liquid outlet pipe through a tenth electromagnetic valve.

6. The apparatus according to claim 5, wherein the flexible tube is bent, and the bent portion of the flexible tube abuts against the inner side wall of the bioreactor, and a metal support member is disposed in the side wall of the flexible tube, and the metal support member is located at the bent portion of the flexible tube.

7. The apparatus according to claim 5, wherein the hose is disposed around an inner sidewall of the bioreactor.

8. A temperature control method of a bioreactor vessel, applied to a temperature control device of a bioreactor vessel as set forth in claim 5, comprising the steps of:

s1, enabling liquid to enter a liquid inlet pipe of the equipment, opening the first electromagnetic valve, keeping the fifth electromagnetic valve closed, enabling the liquid to enter the first TCU through the first electromagnetic valve and the first liquid inlet end, and refrigerating the liquid by the first TCU to form refrigerating liquid;

s2, starting the first liquid pump, enabling the refrigerating fluid to enter the storage fluid tank from the first TCU through the first fluid outlet end, the first liquid pump and the refrigerating fluid inlet end for storage, and measuring the temperature of the refrigerating fluid in the storage fluid tank to obtain a detection temperature;

s3, after the detected temperature reaches the reaction temperature required in the biological reaction container, the first liquid pump stops working, the second liquid pump and the third electromagnetic valve are started, and refrigerating fluid enters the hose from the liquid storage tank through the refrigerating liquid outlet end, the second liquid pump, the third electromagnetic valve and the container liquid inlet pipe to cool reactants in the biological reaction container;

s4, opening the fourth electromagnetic valve, and enabling the refrigerating fluid to form cold return fluid after heat exchange with reactants in the biological reaction container, wherein the cold return fluid is discharged from the container liquid discharge pipe and enters the storage fluid tank through the fourth electromagnetic valve and the refrigerating return fluid end to form circulation.