CN117066237A - Cell culture bottle is retrieved with bottle washing system - Google Patents

Cell culture bottle is retrieved with bottle washing system Download PDF

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Publication number
CN117066237A
CN117066237A CN202311345679.3A CN202311345679A CN117066237A CN 117066237 A CN117066237 A CN 117066237A CN 202311345679 A CN202311345679 A CN 202311345679A CN 117066237 A CN117066237 A CN 117066237A
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CN
China
Prior art keywords
sealing plate
cell culture
upper sealing
shell
water
Prior art date
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Granted
Application number
CN202311345679.3A
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Chinese (zh)
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CN117066237B (en
Inventor
杨君竹
张相平
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Jiannuowei Biotechnology Jiangsu Co ltd
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Jiannuowei Biotechnology Jiangsu Co ltd
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Priority to CN202311345679.3A priority Critical patent/CN117066237B/en
Publication of CN117066237A publication Critical patent/CN117066237A/en
Application granted granted Critical
Publication of CN117066237B publication Critical patent/CN117066237B/en
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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00Cleaning hollow articles by methods or apparatus specially adapted thereto 
    • B08B9/08Cleaning containers, e.g. tanks
    • B08B9/093Cleaning containers, e.g. tanks by the force of jets or sprays
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B13/00Accessories or details of general applicability for machines or apparatus for cleaning
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/50Reuse, recycling or recovery technologies
    • Y02W30/62Plastics recycling; Rubber recycling

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of cleaning, in particular to a bottle washing system for recycling cell culture bottles, which comprises a lower shell, an upper sealing plate and a lower sealing plate, wherein a water spraying rod is arranged in the lower shell; the upper sealing plate is provided with a one-way valve; a liquid chamber is arranged in the lower shell and below the lower sealing plate, and cleaning liquid is filled in the liquid chamber; the upper shell can be reversely buckled on the lower shell in an up-and-down sliding manner to form a closed environment, so that the cleaning liquid is prevented from splashing to cause external environmental pollution during cleaning, and the upper shell is connected with the upper sealing plate and drives the upper sealing plate to reciprocate up and down, so that the cleaning liquid below the lower sealing plate is extruded to enter the cell culture flask for cleaning through the water spray rod. The waste liquid after washing enters the gas chamber through the one-way valve, and the liquid before and after washing can not be mixed, so that the cleaning liquid is prevented from being polluted, and the cleaning effect is further improved.

Description

Cell culture bottle is retrieved with bottle washing system
Technical Field
The invention relates to the technical field of cleaning, in particular to a bottle washing system for recycling a cell culture bottle.
Background
The cell culture bottle needs to be cleaned and disinfected after being used, the cleaning modes are generally two, the cell culture bottle needs to be cleaned in batches by means of equipment during large-batch cleaning, and the cell culture bottle needs to be cleaned manually for saving water during small-batch bottle cleaning. In the prior art, there is a way to clean the inside of the bottle by flushing the bottle upside down. The cleaning mode is convenient and quick, waste liquid is easy to splash in the cleaning process, the experimental environment is polluted, and the cleaned waste water can be mixed with purified water, so that the flushing effect is affected, and the water saving is not facilitated.
Disclosure of Invention
The invention provides a bottle washing system for recycling a cell culture bottle, which aims to solve the problems of poor cleaning effect and water consumption in the prior art when cleaning the bottle.
The invention relates to a bottle washing system for recovering a cell culture bottle, which adopts the following technical scheme:
the bottle washing system for recovering the cell culture bottle comprises a lower shell, an upper sealing plate and a lower sealing plate, wherein a water spraying rod is arranged in the lower shell, is vertically arranged and is fixed on the lower shell; the water spray rod is hollow, and a water inlet communicated with the interior of the water spray rod is arranged at the position of the water spray rod at the bottom of the lower shell; the upper sealing plate and the lower sealing plate are both sleeved on the water spray rod and are both in sliding seal with the outer peripheral surface of the water spray rod and the inner wall surface of the lower shell, and the upper sealing plate is positioned above the lower sealing plate and is used for limiting a sealed gas chamber with the lower sealing plate; the upper sealing plate is provided with a one-way valve which allows fluid to flow to the gas chamber in one way; a liquid chamber is arranged in the lower shell and below the lower sealing plate, and cleaning liquid is filled in the liquid chamber; the upper end of the water spray rod is positioned above the upper sealing plate, and a water spray port is arranged at the upper end of the water spray rod; the upper sealing plate is provided with a clamping mechanism, the cell culture bottle is reversely buckled on the upper sealing plate through the clamping mechanism, and the upper end of the water spraying rod extends into the cell culture bottle; the upper shell can be reversely buckled on the lower shell in a vertical sliding way, and the upper shell is connected with the upper sealing plate and drives the upper sealing plate to reciprocate up and down, so that cleaning liquid below the lower sealing plate is extruded to enter the cell culture flask for cleaning through the water spray rod.
Further, the upper sealing plate and the lower sealing plate are in spiral fit with the water spraying rod so as to rotate when the upper sealing plate moves up and down along with the upper shell.
Further, the inner wall of the lower shell is provided with a water spraying pipe which is communicated with the liquid chamber and the upper sealing plate and used for guiding the cleaning liquid to flow upwards to clean the outer surface of the cell culture flask.
Further, the spray pipes are multiple, circumferentially distributed around the spray rod, and the first spray heads are installed at the communication positions of the upper sealing plate and the upper part of each spray pipe.
Further, the clamping mechanism comprises a chuck and a clamping jaw piece, the clamping jaw piece comprises a bottom ring and a plurality of ejector rods, and the bottom ring and the chuck are sleeved outside the water spraying rod and are detachably arranged on the upper sealing plate; the ejector rods are circumferentially distributed around the bottom ring and are fixed on the bottom ring, and the ejector rods are elastic pieces capable of deforming and are used for clamping the body of the cell culture flask; the chuck is located in the bottom ring, and a clamping block for clamping the bottle mouth of the cell culture bottle is arranged on the chuck.
Further, a gas release valve is mounted on the lower housing in communication with the gas chamber, the gas release valve having an open state in which the gas chamber is allowed to communicate with the outside of the lower housing and a closed state in which the gas release valve isolates the gas chamber from the outside of the lower housing.
Further, the upper shell is fixedly connected with a compression bar, the lower end of the compression bar is connected with a first magnetic block, a second magnetic block is fixedly installed on the upper sealing plate, and when the upper shell is reversely buckled on the lower shell, the first magnetic block and the second magnetic block are attracted to each other, so that the upper sealing plate moves up and down along with the upper shell synchronously.
Further, a water filling port and a water outlet are formed in the side wall of the lower shell, and the water outlet is positioned above the water filling port.
Further, a second spray head is arranged at the water spraying port at the upper end of the water spraying rod.
Further, a handle which is convenient for gripping is arranged on the upper shell.
The beneficial effects of the invention are as follows: the upper shell of the bottle washing system for recycling the cell culture bottles is reversely buckled with the lower shell to form a closed environment, so that the cell culture bottles are cleaned in the closed environment, the external environment pollution caused by splashing of cleaning liquid during cleaning is avoided, the system is suitable for cleaning small-batch cell culture bottles, and the water consumption is low. When the upper shell drives the upper sealing plate to move downwards, the upper sealing plate pushes the lower sealing plate to move downwards through pushing the gas chamber, the lower sealing plate pushes the liquid chamber, so that cleaning liquid enters the water spray rod from the water inlet and is sprayed out from the water spray port to flush the inside of the cell culture bottle, and the upper sealing plate moves up and down to drive the cell culture bottle to move up and down, so that the water spray port can flush different positions in the cell culture bottle, and the cleaning effect is better; the waste liquid after washing enters the gas chamber through the one-way valve, and the liquid before and after washing can not be mixed, so that the cleaning liquid is prevented from being polluted, and the cleaning effect is further improved.
Further, the size of the gas chamber can be adjusted by arranging the air release valve, and then the cleaning liquid extruded by the lower sealing plate is adjusted to be pressed down each time, so that the consumption of the cleaning liquid can be controlled according to actual use conditions, the size of the gas chamber can be adjusted according to different bottle body sizes, and the cleaning liquid can be effectively cleaned and flushed at different positions in a larger range in the cell culture bottle under the condition of the same water yield.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the invention, and that other drawings can be obtained according to these drawings without inventive faculty for a person skilled in the art.
FIG. 1 is a schematic diagram showing the overall structure of an embodiment of a bottle washing system for recovering a cell culture bottle according to the present invention;
FIG. 2 is an exploded view showing the overall structure of an embodiment of a bottle washing system for cell culture bottle recovery according to the present invention;
FIG. 3 is a top view of an embodiment of a system for washing a cell culture flask in accordance with the present invention;
FIG. 4 is a schematic diagram showing the structure of a chuck in an embodiment of a bottle washing system for recovering a cell culture bottle according to the present invention;
FIG. 5 is a schematic view in section in the direction A-A of FIG. 3;
FIG. 6 is a schematic partial cutaway view of an embodiment of a system for washing a cell culture flask in accordance with the invention;
in the figure: 100. an upper housing; 110. a compression bar; 120. a first magnetic block; 130. a handle; 200. a lower housing; 210. a water spray rod; 211. a water inlet; 213. a second nozzle; 220. a release valve; 230. a water filling port; 240. a water outlet; 300. a clamping mechanism; 310. a chuck; 311. a clamping block; 320. a jaw member; 321. a bottom ring; 322. a push rod; 400. an upper sealing plate; 410. a one-way valve; 500. a lower sealing plate; 600. a water spray pipe; 610. a first spray head.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
An embodiment of a bottle washing system for recovering a cell culture bottle of the present invention, as shown in fig. 1 to 6, includes a lower housing 200, an upper housing 100, an upper sealing plate 400, and a lower sealing plate 500.
A water spray rod 210 is arranged in the lower shell 200, the water spray rod 210 is vertically arranged, and the lower end of the water spray rod is fixed on the lower shell 200; the water spray bar 210 is hollow inside, and a water inlet 211 communicating with the inside of the water spray bar 210 is provided at the lower end of the water spray bar 210. The bottom of the lower housing 200 is provided with an ear plate by which the lower housing 200 may be secured to a table top or other location.
The upper sealing plate 400 and the lower sealing plate 500 are sleeved on the water spraying rod 210 and are in sliding seal with the outer peripheral surface of the water spraying rod 210 and the inner wall surface of the lower shell 200, and the upper sealing plate 400 is positioned above the lower sealing plate 500 and defines a sealed gas chamber with the lower sealing plate 500; the upper seal plate 400 is provided with a one-way valve 410 that allows fluid to flow in one direction to the gas chamber; a liquid chamber is arranged in the lower shell 200 and below the lower sealing plate 500, and cleaning liquid is filled in the liquid chamber; the upper end of the water spray bar 210 is located above the upper seal plate 400, and the upper end of the water spray bar 210 is provided with water spray ports.
The upper sealing plate 400 is provided with a clamping mechanism 300, the cell culture bottle is reversely buckled on the upper sealing plate 400 through the clamping mechanism 300, and the upper end of the water spraying rod 210 extends into the cell culture bottle.
The upper casing 100 is reversely buckled on the lower casing 200 in a vertically sliding manner, and the upper casing 100 is connected with the upper sealing plate 400 and drives the upper sealing plate 400 to reciprocate up and down, so that cleaning fluid below the lower sealing plate 500 is extruded to enter the cell culture flask for cleaning through the water spray rod 210.
The upper shell 100 is reversely buckled with the lower shell 200 to form a closed environment, so that the cell culture flask is cleaned in the closed environment, the external environment pollution caused by splashing of cleaning liquid during cleaning is avoided, and the system is suitable for cleaning small-batch cell culture flasks and has low water consumption. When the upper shell 100 drives the upper sealing plate 400 to move downwards, the upper sealing plate 400 pushes the lower sealing plate 500 to move downwards through the extrusion gas chamber, the lower sealing plate 500 extrudes the liquid chamber, so that cleaning liquid enters the water spray rod 210 from the water inlet 211 and is sprayed out from the water spray port to flush the inside of the cell culture bottle, and the upper sealing plate 400 moves up and down to drive the cell culture bottle to move up and down, so that the water spray port can flush different positions in the cell culture bottle, and the cleaning effect is better; the waste liquid after flushing enters the gas chamber through the one-way valve 410, and the liquid before and after cleaning is not mixed, so that the cleaning liquid is prevented from being polluted, and the cleaning effect is further improved.
In this embodiment, the upper sealing plate 400 and the lower sealing plate 500 are in spiral fit with the water spraying rod 210, so that the upper sealing plate 400 rotates when moving up and down along with the upper housing 100, and further drives the cell culture flask to rotate, so that cleaning is more uniform, and dirt on the inner wall of the cell culture flask can be discharged by rotating the cell culture flask. Specifically, the outer circumference of the water spray rod 210 is provided with a spiral groove, the upper sealing plate 400 and the lower sealing plate 500 are sleeved on the inner ring side wall of the water spray rod 210 and are respectively provided with a protrusion, the protrusions are matched with the spiral groove in a sliding sealing manner, and when the upper sealing plate 400 and the lower sealing plate 500 move up and down, the protrusions slide in the spiral groove to enable the upper sealing plate 400 and the lower sealing plate 500 to rotate.
In this embodiment, the inner wall of the lower housing 200 is provided with a water spray pipe 600, and the water spray pipe 600 is communicated with the liquid chamber and the upper sealing plate 400 for guiding the cleaning liquid to flow upwards to clean the outer surface of the cell culture flask.
In this embodiment, the number of the spray nozzles 600 is plural, the spray nozzles 600 are circumferentially distributed around the spray rod 210, and the first spray nozzles 610 are installed at the communication position above the upper sealing plate 400, each first spray nozzle 610 corresponds to a certain angle for flushing the outer circumference of the cell culture flask, and the spray range of the plurality of first spray nozzles 610 can cover the whole outer circumference of the cell culture flask by setting the plurality of spray nozzles 600.
In this embodiment, the clamping mechanism 300 includes a chuck 310 and a clamping jaw member 320, the clamping jaw member 320 includes a bottom ring 321 and a plurality of top rods 322, and the bottom ring 321 and the chuck 310 are both sleeved outside the water spray rod 210 and are both detachably mounted on the upper sealing plate 400; the ejector rods 322 are circumferentially distributed around the bottom ring 321 and are fixed on the bottom ring 321, and the ejector rods 322 are elastic members capable of deforming and are used for clamping the body of the cell culture flask. Specifically, the lower ends of the ejector rods 322 are fixedly connected with the bottom ring 321, the upper ends of the ejector rods 322 are far away from each other, the cell culture flask is generally of a conical structure with a small bottleneck and a large flask bottom, the flask body pushes the upper ends of the ejector rods 322 away from each other when the flask body is inverted, and the ejector rods 322 clamp the flask body under the action of restoring deformation. The chuck 310 is positioned in the bottom ring 321, a clamping block 311 for clamping the bottle mouth of the cell culture bottle is arranged on the chuck 310, the clamping block 311 is an elastic piece capable of deforming, and the lower end of the clamping block 311 is fixedly connected with the chuck 310; the fixture blocks 311 are multiple, are distributed around the circumference interval of the chuck 310, when the cell culture bottle is back-buckled, the upper ends of the plurality of fixture blocks 311 are pushed away from by the bottle mouth, the bottle mouth is clamped by the plurality of fixture blocks 311 under the action of restoring deformation, the upper ends of the fixture blocks 311 are in a reversed hook shape, and the barb-shaped structures of the fixture blocks 311 are back-buckled at the edge of the bottle mouth when the fixture blocks 311 clamp the bottle mouth, so that the cell culture bottle is prevented from being separated from the chuck 310. The waste liquid after rinsing flows from the space between the chuck 310, the bottom ring 321 and the upper closure plate 400 to the upper closure plate 400.
In the present embodiment, the lower case 200 is mounted with the gas release valve 220 in communication with the gas chamber, the gas release valve 220 having an opened state in which the gas release valve 220 allows communication between the gas chamber and the outside of the lower case 200 and blocks the gas outside the lower case 200 from entering the gas chamber, and a closed state in which the gas release valve 220 isolates the gas chamber from the outside of the lower case 200. The air release valve 220 moves the upper sealing plate 400 downward in an opened state to press the gas in the gas chamber to be discharged, so that the distance between the upper sealing plate 400 and the lower sealing plate 500 is reduced, and the initial gas amount of the gas chamber is reduced. When flushing is started, the air release valve 220 is in a closed state, the gas chamber is isolated from the outside of the lower shell 200, and the smaller the initial gas amount of the gas chamber, the more cleaning fluid is extruded and sprayed through the lower sealing plate 500 under the same downward movement amplitude of the upper sealing plate 400; that is, the size of the gas chamber can be adjusted by the air release valve 220, so that the cleaning liquid extruded by the lower sealing plate 500 is adjusted each time, the consumption of the cleaning liquid can be controlled according to the actual use condition, and the cleaning liquid can be effectively saved. A cap may be disposed at a position of the lower housing 200 where the air release valve 220 is installed, the air release valve 220 is in a closed state by screwing the cap, and the air release valve 220 is in an opened state by removing the cap.
In this embodiment, a pressing rod 110 is fixedly connected to the upper housing 100, a first magnetic block 120 is connected to the lower end of the pressing rod 110, a second magnetic block (not shown in the drawing) is fixedly mounted on the upper sealing plate 400, and when the upper housing 100 is fastened to the lower housing 200 in an inverted manner, the first magnetic block 120 and the second magnetic block attract each other, so that the upper sealing plate 400 moves up and down synchronously with the upper housing 100. When the upper housing 100 is required to be separated, the first magnetic block 120 and the second magnetic block are separated by rotating the upper housing 100, and then the upper housing 100 is lifted upwards. Through the magnetic block connection, the upper shell 100 and the upper sealing plate 400 can be connected and separated quickly, and the service time is saved.
In this embodiment, the sidewall of the lower housing 200 is provided with a water filling port 230 and a water outlet 240, and the water outlet 240 is located above the water filling port 230. Both the water filling port 230 and the water outlet port 240 can be opened and closed as needed.
In this embodiment, the second nozzle 213 is installed at the water spraying port at the upper end of the water spraying rod 210, and the second nozzle 213 has a structure in the prior art, and can be an annular nozzle capable of spraying the bottom and the peripheral wall of the cell culture flask at the same time.
In this embodiment, the upper case 100 is mounted with a grip 130 for easy grip.
Before the bottle washing system for recycling the cell culture bottle is used, the water filling port 230, the water outlet 240 and the air release valve 220 are all in a closed state, cleaning liquid is filled into the liquid chamber by opening the water filling port 230, the lower sealing plate 500 moves upwards under the buoyancy effect of the cleaning liquid and is always positioned above the liquid level, and the lower sealing plate 500 pushes the upper sealing plate 400 to synchronously move upwards by extruding the gas chamber until the gas chamber is positioned at a position capable of being communicated with the air release valve 220. Then, the water filling port 230 is closed, the cell culture flask is reversely buckled on the clamping mechanism 300 on the upper sealing plate 400, then the upper shell 100 is reversely buckled on the lower shell 200, the upper shell 100 and the lower shell 200 form a closed space, the first magnetic block 120 on the upper shell 100 and the second magnetic block on the upper sealing plate 400 are attracted, then the air release valve 220 is in an open state, the upper sealing plate 400 is driven to move up and down by the upper shell 100, the initial size of the gas chamber is adjusted according to requirements, and the air release valve 220 is in a closed state after adjustment. Pulling the upper case 100 to move downward and then to move upward, and making the amplitude of each downward movement consistent with the amplitude of the upward movement as much as possible; when the upper sealing plate 400 moves downwards, the lower sealing plate 500 is pushed to move downwards by extruding the gas chamber, the lower sealing plate 500 extrudes the liquid chamber, so that part of cleaning liquid enters the water spray rod 210 from the water inlet 211 and is sprayed out from the water spray port to flush the inside of the cell culture bottle, and part of cleaning liquid flows upwards through the water spray pipe 600 and is sprayed out from the first spray nozzle 610 to flush the body of the cell culture bottle. When the upper sealing plate 400 moves upwards, the waste liquid after flushing is caused to enter the gas chamber through the one-way valve 410, and the liquid before and after cleaning is not mixed, so that the cleaning liquid is prevented from being polluted, and the cleaning effect is further improved. The upper sealing plate 400 rotates under the spiral cooperation with the water spray rod 210 when moving up and down, and drives the cell culture bottle to move up and down and rotate, so that the water spray port can flush different positions in the cell culture bottle, and the cleaning effect is better.
Each time the upper sealing plate 400 moves downwards to push the lower sealing plate 500 to move downwards by a certain extent, the lower sealing plate 500 moves downwards until the gas chamber can be communicated with the water outlet 240, and the water outlet 240 is opened to discharge waste liquid in the gas chamber.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.

Claims (10)

1. A bottle washing system for cell culture bottle recovery, which is characterized in that: comprises a lower shell (200), an upper shell (100), an upper sealing plate (400) and a lower sealing plate (500);
a water spray rod (210) is arranged in the lower shell (200), and the water spray rod (210) is vertically arranged and fixed on the lower shell (200); the water spray rod (210) is hollow, and a water inlet (211) communicated with the interior of the water spray rod (210) is arranged at the position of the water spray rod (210) at the bottom of the lower shell (200);
the upper sealing plate (400) and the lower sealing plate (500) are sleeved on the water spraying rod (210) and are in sliding seal with the outer peripheral surface of the water spraying rod (210) and the inner wall surface of the lower shell (200), and the upper sealing plate (400) is positioned above the lower sealing plate (500) and is defined with a sealed gas chamber between the lower sealing plate (500); the upper sealing plate (400) is provided with a one-way valve (410) which allows fluid to flow to the gas chamber in one way; a liquid chamber is arranged in the lower shell (200) and below the lower sealing plate (500), and cleaning liquid is filled in the liquid chamber; the upper end of the water spray rod (210) is positioned above the upper sealing plate (400), and a water spray port is arranged at the upper end of the water spray rod (210);
the upper sealing plate (400) is provided with a clamping mechanism (300), the cell culture bottle is reversely buckled on the upper sealing plate (400) through the clamping mechanism (300), and the upper end of the water spraying rod (210) extends into the cell culture bottle;
the upper shell (100) can be reversely buckled on the lower shell (200) in an up-down sliding way, the upper shell (100) is connected with the upper sealing plate (400) and drives the upper sealing plate (400) to reciprocate up and down, and cleaning liquid below the lower sealing plate (500) is extruded to enter the cell culture flask for cleaning through the water spraying rod (210).
2. The system for washing a cell culture flask according to claim 1, wherein: the upper sealing plate (400) and the lower sealing plate (500) are in spiral fit with the water spray rod (210) so as to rotate when the upper sealing plate (400) moves up and down along with the upper shell (100).
3. The system for washing a cell culture flask according to claim 1, wherein: the inner wall of the lower shell (200) is provided with a spray pipe (600), and the spray pipe (600) is communicated with the liquid chamber and the upper part of the upper sealing plate (400) and is used for guiding the cleaning liquid to flow upwards to clean the outer surface of the cell culture flask.
4. A cell culture flask recycling bottle washing system according to claim 3, wherein: the spray pipes (600) are distributed circumferentially around the spray rod (210), and a first spray head (610) is arranged at the communication position of each spray pipe (600) and the upper part of the upper sealing plate (400).
5. The system for washing a cell culture flask according to claim 1, wherein: the clamping mechanism (300) comprises a chuck (310) and a clamping jaw piece (320), wherein the clamping jaw piece (320) comprises a bottom ring (321) and a plurality of ejector rods (322), and the bottom ring (321) and the chuck (310) are sleeved outside the water spraying rod (210) and are detachably arranged on the upper sealing plate (400); the ejector rods (322) are circumferentially distributed around the bottom ring (321) and are fixed on the bottom ring (321), and the ejector rods (322) are elastic pieces capable of deforming and are used for clamping the body of the cell culture flask; the chuck (310) is positioned in the bottom ring (321), and a clamping block (311) for clamping the bottle mouth of the cell culture bottle is arranged on the chuck (310).
6. The system for washing a cell culture flask according to claim 1, wherein: the lower housing (200) is provided with a gas release valve (220) communicated with the gas chamber, the gas release valve (220) is provided with an opening state and a closing state, the gas release valve (220) allows the gas chamber to be communicated with the outside of the lower housing (200) in the opening state, and the gas release valve (220) isolates the gas chamber from the outside of the lower housing (200) in the closing state.
7. The system for washing a cell culture flask according to claim 1, wherein: the upper shell (100) is internally fixedly connected with a pressing rod (110), the lower end of the pressing rod (110) is connected with a first magnetic block (120), a second magnetic block is fixedly installed on the upper sealing plate (400), and when the upper shell (100) is reversely buckled on the lower shell (200), the first magnetic block (120) and the second magnetic block are attracted to each other, so that the upper sealing plate (400) moves up and down along with the upper shell (100) synchronously.
8. The system for washing a cell culture flask according to claim 1, wherein: a water filling port (230) and a water outlet (240) are formed in the side wall of the lower shell (200), and the water outlet (240) is located above the water filling port (230).
9. The system for washing a cell culture flask according to claim 1, wherein: a second spray head (213) is arranged at the water spraying port at the upper end of the water spraying rod (210).
10. The system for washing a cell culture flask according to claim 1, wherein: a handle (130) for facilitating gripping is mounted on the upper housing (100).
CN202311345679.3A 2023-10-18 2023-10-18 Cell culture bottle is retrieved with bottle washing system Active CN117066237B (en)

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CN117066237B CN117066237B (en) 2023-12-15

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