CN117031717A - Fluorescence microscopy system - Google Patents

Abstract

The present application provides a fluorescence microscopy system comprising: the fluorescence acquisition mechanism comprises a fixed bracket and a plurality of single-path optical machines arranged on the fixed bracket in an array manner; the single-path optical machine is used for exciting and collecting fluorescence of the sample, and the collecting ranges of the single-path optical machines are at least partially not overlapped; the carrying mechanism comprises a carrying platform which is positioned below the plurality of single-path optical machines and used for carrying the samples, and a driving mechanism for driving the carrying platform to move along at least one direction. In the scheme, the fluorescence acquisition mechanism for acquiring the fluorescence of the sample is formed by adopting a plurality of single-path optical machines, so that the acquisition range of the fluorescence acquisition mechanism is improved. In addition, in the process of sample collection, a plurality of single-path optical machines are adopted to be fixed, and a sample carrying mechanism moves, so that the safety of the plurality of single-path optical machines can be greatly improved, and meanwhile, the stability in the collection process is also improved.

Description

Fluorescence microscopy system

Technical Field

The application relates to the technical field of nucleic acid detection, in particular to a fluorescence microscopy system.

Background

In the process of nucleic acid detection, tens of thousands of nucleic acid molecules need to be subjected to fluorescence detection, and how to realize the detection process quickly and at low cost is a difficulty of the current fluorescence detection technology. In the existing fluorescence microscopic imaging system, a single-path optical machine is used for detecting samples, the imaging area of the single-path optical machine is limited, the detection efficiency is low, the detection time is greatly increased, the detection data flux of the single-path optical machine is limited, the detection labor and reagent cost are increased, and the detection cost is high.

Disclosure of Invention

The application provides a fluorescence microscopy system which improves the effect of testing a sample.

The present application provides a fluorescence microscopy system comprising:

the fluorescence acquisition mechanism comprises a fixed bracket and a plurality of single-path optical machines arranged on the fixed bracket in an array manner; the single-path optical machine is used for exciting and collecting fluorescence of the sample, and the collecting ranges of the single-path optical machines are at least partially not overlapped;

the carrying mechanism comprises a carrying platform which is positioned below the plurality of single-path optical machines and used for carrying the samples, and a driving mechanism for driving the carrying platform to move along at least one direction.

In the scheme, the fluorescence acquisition mechanism for acquiring the fluorescence of the sample is formed by adopting a plurality of single-path optical machines, so that the acquisition range of the fluorescence acquisition mechanism is improved. In addition, in the process of sample collection, a plurality of single-path optical machines are adopted to be fixed, and a sample carrying mechanism moves, so that the safety of the plurality of single-path optical machines can be greatly improved, and meanwhile, the stability in the collection process is also improved.

In a specific implementation manner, the number of the single-path light machines is four, and the four single-path light machines are arranged in a rectangular array.

In a specific embodiment, each of the single-pass optical engines comprises:

an excitation light source assembly for emitting light that excites fluorescence of the sample;

a focusing light source assembly for emitting a first focusing light ray irradiated to the sample;

the objective lens is used for transmitting the light rays emitted by the excitation light source assembly to the sample and receiving fluorescence excited by the sample; and propagating the first focusing light to the sample and receiving a second focusing light reflected by the sample;

imaging means for collecting fluorescence excited by the sample; focusing and imaging according to the second focusing light rays;

the first dichroic mirror is used for reflecting the light rays emitted by the excitation light source component and the first focusing light rays to the objective lens; and transmitting the fluorescence excited by the sample and the second focusing light to the imaging device.

In a specific embodiment, each of the single-pass optical engines further comprises: the first double-bandpass filter is positioned between the first dichroic mirror and the imaging device and is used for filtering illumination light doped in fluorescence excited by the sample and transmitting the fluorescence excited by the filtered sample to the imaging device.

In a specific embodiment, the single-path optical machine further comprises a cylindrical lens located between the imaging device and the first dual-bandpass filter, and a plurality of lenses are arranged in the cylindrical lens; the lenses are used for focusing fluorescence excited by the filtered sample to the imaging device through the first double-bandpass filter.

In a specific embodiment, the excitation light source assembly includes: a first light source for emitting light of a first wavelength, and a second light source for emitting light of a second wavelength; further comprises: a second dichroic mirror for transmitting the first wavelength light and reflecting the second wavelength light; and a second dual bandpass filter for filtering fluorescence bands in the first wavelength light and the second wavelength light.

In a specific embodiment, the focusing light source assembly includes: a laser emission source; an aperture diaphragm located on a propagation light path of the laser emitted by the laser emission source; and a third dichroic mirror for reflecting the laser light emitted from the laser light emitting source and transmitting the first wavelength light and the second wavelength light filtered by the second dual-band pass filter.

In a specific embodiment, the drive mechanism comprises: the first guide rail is fixed relative to the fixed support, the base is slidably assembled on the first guide rail, and the carrying platform is slidably assembled on the base; wherein,

the sliding direction of the base is intersected with the sliding direction of the carrying platform; and the sliding direction of the base and the sliding direction of the carrying platform are respectively parallel to the surfaces of the plurality of single-path optical machines facing the sample.

In a specific embodiment, the fluorescence microscopy system further comprises a support, the fixed support being fixedly connected to the support;

the first guide rail is fixed on the supporting seat.

In a specific embodiment, the longitudinal portion of the first guide rail is located outside the plurality of single-path optical machines, and the carrying platform is located outside the plurality of single-path optical machines when the supporting seat slides to the first setting position.

Drawings

FIG. 1 is a schematic diagram of a fluorescence microscope system according to an embodiment of the present application;

fig. 2 is a schematic diagram of a single-path optical machine according to an embodiment of the present application.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present application will be further described in detail with reference to the accompanying drawings.

It is noted that unless otherwise defined, technical or scientific terms used in one or more embodiments of the present disclosure should be taken in a general sense as understood by one of ordinary skill in the art to which the present disclosure pertains. The use of the terms "first," "second," and the like in one or more embodiments of the present description does not denote any order, quantity, or importance, but rather the terms "first," "second," and the like are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that elements or items preceding the word are included in the element or item listed after the word and equivalents thereof, but does not exclude other elements or items. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", etc. are used merely to indicate relative positional relationships, which may also be changed when the absolute position of the object to be described is changed.

In order to facilitate understanding of the fluorescence microscope system provided by the embodiment of the application, an application scene of the fluorescence microscope system is first described. The fluorescence microscopy system provided by the embodiment of the application is used for carrying out fluorescence detection on the sample. The current detection mode is to detect the sample by adopting a single-path optical machine, but the imaging area of the single-path optical machine is limited, so that the detection efficiency is lower and the detection time is longer. Therefore, the embodiment of the application provides a fluorescence microscopy system for improving the efficiency of sample detection. The following detailed description is made with reference to the specific drawings and examples.

Referring to fig. 1, fig. 1 shows an overall structure diagram of a fluorescence microscopy system provided by an embodiment of the application. The fluorescence microscopy system provided by the embodiment of the application is mainly divided into two parts: fluorescence acquisition mechanism 1000 and carrier mechanism 2000. The fluorescence acquisition mechanism 1000 comprises a fixed bracket 200 and a plurality of single-path optical machines 100 arranged on the fixed bracket 200 in an array manner; the single-path optical machines 100 are used for exciting and collecting fluorescence of the sample 300, and collecting ranges of the single-path optical machines 100 are at least partially not overlapped. The loading mechanism 2000 includes a loading platform 2100 located under the plurality of single-path optical machines 100 and used for carrying the sample, and a driving mechanism (not shown) for driving the loading platform 2100 to move along at least one direction.

The multiple single-path optical machines 100 of the fluorescence collection mechanism 1000 are used for exciting the sample 300 to emit fluorescence and collecting the fluorescence excited by the sample 300 to complete detection; the loading platform 2100 of the loading mechanism 2000 is used to load the sample 300 and support the sample 300 under the fluorescence collection mechanism 1000. The driving mechanism is used to adjust different areas on the sample 300 to be located under the single-path optical machine 100 so as to image the different areas. By adopting the fluorescence collection mechanism 1000 for collecting sample fluorescence formed by the plurality of single-path photomechanical machines 100, the collection range of the fluorescence collection mechanism is increased, and the problem that the traditional single-path photomechanical machine 100 needs to move for many times when collecting images is avoided. In addition, in the process of sample collection, a plurality of single-path optical machines 1000 are fixed, and the object carrying mechanism 2000 for carrying the samples moves, so that vibration during moving of the single-path optical machines 100 is avoided, stability in the collection process is improved, and detection accuracy and safety of the plurality of single-path optical machines are improved.

In one embodiment, in addition to the fluorescence collection mechanism 1000 and the carrying mechanism 2000, the fluorescence microscope system may further include a support base 3000, where the support base 3000 is used as a support structure to support the fluorescence collection mechanism 1000 and the carrying mechanism 2000, respectively. The respective partial structures of the fluorescence microscope system are described below.

With continued reference to fig. 1, the main structure of the fluorescence microscopy system illustrated in fig. 1 includes a support 3000, a fluorescence acquisition mechanism 1000, and a carrier mechanism 2000. The support base 3000 is a table structure, when the fluorescent light collecting mechanism 1000 and the carrying mechanism 2000 are supported, the fluorescent light collecting mechanism 1000 and the carrying mechanism 2000 are located at the same side of the support base 3000, and the fluorescent light collecting mechanism 1000 and the carrying mechanism 2000 are arranged at intervals along the height direction. In a specific arrangement, the loading mechanism 2000 is close to the support 3000, and the fluorescence acquisition mechanism 1000 is relatively far from the support 3000. In addition, when the carrying mechanism 2000 and the fluorescence collection mechanism 1000 are supported, the fluorescence collection mechanism 1000 is fixed on the supporting seat 3000, and the carrying mechanism 2000 can move relative to the supporting seat 3000, so that the sample 300 carried by the carrying mechanism 2000 can move under the fluorescence collection mechanism 1000, and thus the fluorescence collection mechanism 1000 can collect fluorescence excited by all areas of the sample 300.

It should be understood that, in the embodiment of the present application, the support base 3000 is an optional structure, and the fluorescence microscope system and the carrying mechanism 2000 according to the embodiments of the present application may not be supported by a separate support base 3000. Such as: the carrier mechanism 2000 is supported by other support structures or by the fluorescence acquisition mechanism 1000. For convenience of description, in the embodiment of the present application, the fluorescence microscope system includes the support base 3000.

The fixing support 200 provided in the embodiment of the application is used as a supporting structure to support the single-path optical machine 100, and the single-path optical machine 100 is used for exciting and collecting fluorescence on the sample 300. In the embodiment of the present application, the number of the single-path optical machines 100 is plural, and the plural single-path optical machines 100 are arranged on the fixing support 200 in an array arrangement manner.

With continued reference to fig. 1, the mounting bracket 200 is of an inverted U-shaped configuration with a vertical portion fixedly coupled to the support base 3000 to secure the entire fluorescence acquisition mechanism 1000 to the support base 3000. While the horizontal portion of the fixed bracket 200 serves as a connection structure with the single-track optical bench 100. When a plurality of single-path optical engines 100 are fixed to the fixed bracket 200, an array thereof is arranged on a side of the horizontal portion of the fixed bracket 200 facing away from the support base 3000. It should be understood that a through hole or a hollowed structure for avoiding the collecting part of the single-path optical machine 100 is provided at the horizontal part of the fixing bracket 200. So that the light emitted by each single-path light machine 100 can irradiate the sample 300 carried by the carrying mechanism 2000 and collect the fluorescence excited by the sample 300.

When the plurality of single-path optical machines 100 are fixed on the fixed support 200 in an array manner, the collection ranges of the plurality of single-path optical machines 100 are at least partially not overlapped. So that the collection area of the plurality of single-way photomechanical machines 100 is larger than that of the single-way photomechanical machine 100, and the integral imaging area of the plurality of single-way photomechanical machines 100 is larger than that of the single machine, thereby increasing the flux of detection data, reducing the cost of manpower and reagents for detection and reducing the detection cost.

In addition, the single-path optical machine 100 includes a precise lens and a motor, and the existing fluorescence detection system adopts a scheme of moving the single-path optical machine 100 to scan a sample, so that the risk of damage to the core part of the optical machine core is increased, and the product stability is not facilitated. However, in the fluorescence microscope system provided by the embodiment of the application, the plurality of single-path optical machines 100 are fixedly arranged (fixed on the supporting seat 3000 through the fixing bracket 200), and the solution of relatively moving the carrying mechanism 2000 carrying the sample 300 can reduce the adverse effect caused by the movement of the heavy fluorescence acquisition mechanism 1000. Meanwhile, the fluorescent collection mechanism 1000 is fixedly arranged, so that a cable connected with the single-path optical machine 100 cannot move, and the reliability of the cable and the single-path optical machine 100 in connection is ensured.

In fig. 1, the fluorescence acquisition mechanism 1000 is illustrated as including four single-pass optical engines 100, and the four single-pass optical engines 100 are arranged in a rectangular array. On the one hand, this approach allows for a larger acquisition area while also taking into account the cost of the device itself, to strike a balance between detection efficiency and device cost. Of course, the fluorescence collection mechanism 1000 provided in the embodiment of the present application is not limited to the four single-way optical machines 100 illustrated in the above example, but may be two single-way optical machines 100, three single-way optical machines 100, five single-way optical machines 100, or six single-way optical machines 100 with different numbers of single-way optical machines 100.

Referring to fig. 2 together, fig. 2 shows a schematic diagram of a single-path optical engine 100 according to an embodiment of the present application. The single-path optical machine 100 provided in the embodiment of the present application includes: excitation light source assembly 110, focusing light source assembly 120, objective lens 130, imaging device 170, and first dichroic mirror 140. The excitation light source assembly 110 is used for emitting light for exciting fluorescence of the sample 300, the focusing light source assembly 120 is used for emitting first focusing light for irradiating the sample 300, and the objective lens 130, the first dichroic mirror 140 and the imaging device 170 are part of structures imaged by the light. For convenience of description of the relative positional relationship between the components, the placement direction of the single-path optical bench 100 shown in fig. 2 is taken as a reference direction.

First, describing the objective lens 130 and the imaging device 170, when the objective lens 130 and the imaging device 170 are disposed, the objective lens 130 and the imaging device 170 are disposed along a vertical direction in fig. 2, wherein the objective lens 130 is located at a side close to the sample 300, and the imaging device 170 is relatively far away from the sample 300. The objective lens 130 is used to transmit light to the sample 300 on the one hand and to transmit fluorescence excited by the sample 300 to the imaging device 170 on the other hand. While the imaging device 170 is used to collect and image fluorescence excited by the sample 300, the imaging device 170 may transmit the formed image to a display device or other apparatus to observe the imaging result of the sample 300.

When the objective lens 130 is specifically disposed, the objective lens 130 includes a plurality of lenses, and specifically may include a plurality of convex lenses or a combination of concave lenses, or only a combination of convex lenses, to focus light. Specific lenses in the objective lens 130 are not described in detail in the embodiment of the present application, and conventional lens combination arrangements may be adopted. In particular, when light is transmitted, on the one hand, the light emitted by the excitation light source assembly 110 is transmitted to the sample 300, and after the light excites fluorescence on the sample 300, the objective lens 130 can also receive the fluorescence excited by the sample 300 and transmit the fluorescence to the imaging device 170. In addition, when the focusing light source assembly 120 emits the focusing light, the objective lens 130 may transmit the first focusing light to the sample 300, and receive the second focusing light reflected by the sample 300, and transmit the second focusing light to the imaging device 170. When focusing imaging is required, the driving mechanism connected with the objective lens 130 can drive the objective lens 130 to move along the vertical direction so as to adjust the distance between the objective lens 130 and the imaging device 170, thereby adjusting the focal length, and enabling the imaging device 170 to present a clear image. The driving mechanism may be a driving motor 180, and the specific structure of the driving motor 180 driving the objective lens 130 to perform focusing may be a conventional structure of focusing by a camera, which is not described in detail herein.

The imaging device 170 may be a CCD image sensor (Charge coupled Device, chinese, full: charge coupled device, which may be referred to as a CCD image sensor) for presenting a fluorescence image of the sample 300. In particular use, the imaging device 170 may be used to collect fluorescence excited by the sample 300 for imaging; and focusing imaging according to the second focusing light. During focusing, the position of the objective lens 130 can be determined according to the imaging result of the second focusing light on the imaging device 170, and the objective lens 130 is controlled to perform focusing so as to ensure the effect of fluorescence imaging.

With continued reference to fig. 2, the single-path optical engine 100 further includes a first dual-band-pass filter 150 and a barrel lens 160, where the first dual-band-pass filter 150 and the barrel lens 160 are located between the imaging device 170 and the objective lens 130, and when the first dual-band-pass filter 150 is located on a side of the barrel lens 160 facing away from the imaging device 170, that is, the first dual-band-pass filter 150 is located between the barrel lens 160 and the objective lens 130, and the first dual-band-pass filter 150 is used for filtering illumination light doped in fluorescence excited by the sample 300, and transmitting the fluorescence excited by the filtered sample 300 to the imaging device 170. The cylindrical lens 160 is a light transmission structure, and is internally provided with a plurality of lenses; the plurality of lenses are used to focus the fluorescence excited by the filtered sample 300 by the first dual bandpass filter 150 to the imaging device 170. The plurality of lenses may be convex lenses or concave lenses for converging light rays for imaging on the imaging device 170. The lenses in the barrel lens 160 may be selected to have different lens combinations that co-propagate light with the lenses in the objective lens 130 to propagate light collected by the objective lens 130 into the imaging device 170 for imaging, as desired.

With continued reference to fig. 2, the excitation light source component 110 and the focusing light source component 120 provided in the embodiment of the application are respectively configured to emit different light rays. Wherein, excitation light source assembly 110 includes: a first light source 111 and a second light source 113, wherein the first light source 111 is configured to emit light of a first wavelength and the second light source 113 is configured to emit light of a second wavelength. In a specific arrangement, the light source types of the first light source 111 and the second light source 113 include different light source types of LEDs, lasers, and the like.

In addition, excitation light source assembly 110 also includes a second dichroic mirror 112 and a second dual bandpass filter 114. Wherein the second dichroic mirror 112 is configured to transmit the first wavelength light and reflect the second wavelength light. When the second dichroic mirror 112 is disposed, the first light source 111 and the second light source 113 are disposed on two opposite sides of the second dichroic mirror 112, respectively, the second light beam with the second wavelength emitted by the second light source 113 is reflected to the second dual-bandpass filter 114 by the second dichroic mirror 112, and the first light beam with the first wavelength emitted by the first light source 111 is transmitted to the second dual-bandpass filter 114 by the second dichroic mirror 112. The second dual-band filter 114 is used for filtering out fluorescence bands in the first wavelength light and the second wavelength light, so as to avoid interference between fluorescence signals excited by the sample 300 and light source signals (light emitted by the first light source 111 and the second light source 113), and ensure imaging effect.

The focusing light source assembly 120 includes a laser light emitting source 123, and an aperture stop 122 located on a propagation path of laser light emitted from the laser light emitting source 123. The focusing light source assembly 120 further includes a third dichroic mirror 121, and the third dichroic mirror 121 is configured to reflect the laser light emitted by the laser light emitting source 123 and transmit the first wavelength light and the second wavelength light filtered by the second dual band-pass filter 114. As shown in fig. 2, the third dichroic mirror 121 is located on a side of the second dual bandpass filter 114 facing away from the second dichroic mirror 112. In use, the first wavelength light and the second wavelength light filtered by the second dual bandpass filter 114 are transmitted through the third dichroic mirror 121 and then continue to propagate. Wherein, the laser emission source 123 adopts a laser diode as an emission light source, and the aperture diaphragm 122 is a circular eccentric through hole. Light emitted from the laser diode passes through the aperture stop 122 and is reflected by the third dichroic mirror 121.

With continued reference to fig. 2, the first dichroic mirror 140 is configured to reflect the light emitted by the excitation light source component 110 and the first focusing light emitted by the focusing light source component 120 to the objective lens 130; and transmits fluorescence excited by the sample 300 and second focusing light formed by reflection of the first focusing light to the imaging device 170. As shown in fig. 2, first dichroic mirror 140 is positioned between objective 130 and imaging device 170. When the single-path optical bench 100 includes the barrel lens 160 and the first dual-band-pass filter 150, the first dichroic mirror 140 is located between the first dual-band-pass filter 150 and the objective lens 130, that is, when the placement direction of the single-path optical bench 100 shown in fig. 2 is taken as the reference direction, the imaging device 170, the first dual-band-pass filter 150, the first dichroic mirror 140, and the objective lens 130 are sequentially arranged from top to bottom in the vertical direction.

In addition, the single-path optical engine 100 further includes a lens 190, and the lens 190 is disposed between the third dichroic mirror 121 and the first dichroic mirror 140, so as to transmit the first focusing light, the first wavelength light and the second wavelength light filtered by the second dual-band-pass filter 114 to the first dichroic mirror 140. The lens 190 is a convex lens, and specifically acts as a collimating lens. In use, light is collected and the angle at which the light is incident on first dichroic mirror 140 is adjusted to ensure that the light is reflected by first dichroic mirror 140 and propagates into objective 130.

As can be seen from the above description, in use, the excitation light (the first wavelength light and the second wavelength light) emitted by the first light source 111 and the second light source 113 passes through the second dichroic mirror 112 and enters the second dual band pass filter 114, and the second dual band pass filter 114 mainly filters out the emission band of fluorescence, so as to avoid interference between the fluorescence signal and the light source signal. The excitation light passing through the second bandpass filter 114 enters the lens through the third dichroic mirror 121, is then reflected by the first dichroic mirror 140 (which is a bandpass dichroic mirror), enters the objective lens 130, and finally reaches the surface of the sample 300. The fluorescence wavelength in the excitation sample 300 is red shifted or blue shifted, the fluorescence signal is collected by the high power objective 130, the illumination light in the fluorescence signal is further filtered by the first dichroic mirror 140 entering the first dual bandpass filter 150, and the fluorescence signal is imaged to the imaging device 170 after passing through the cylindrical mirror 160.

When focusing is performed, the laser emission source 123 adopts a laser diode as an emission light source, the aperture diaphragm 122 is a circular eccentric through hole, a first focusing light beam emitted by the laser diode passes through the aperture diaphragm 122, is reflected by the third dichroic mirror 121, enters the lens 190 to be collimated, and enters the objective lens 130 after being reflected by the first dichroic mirror 140, is focused by the objective lens 130 and reaches the surface of the sample 300, the reflected first focusing light beam on the surface of the sample 300 forms a second focusing light beam, the second focusing light beam is collimated after being collected by the objective lens 130, passes through the first dichroic mirror 140 and the first dual bandpass filter 150, and is focused on the imaging device 170 after passing through the barrel lens 160.

The sample 300 is positioned on a carriage 2000. The carriage 2000 can move the sample 300 in at least one direction and scan under the objective 130 for imaging. Before scanning, the driving motor 180 is controlled to drive the objective 130 to move up and down to find the best focal plane of the objective 130 according to the central coordinate of the light spot on the imaging device 170, so as to realize the focusing function. When the object carrying mechanism 2000 moves the sample to different scanning areas, the best focal plane can be quickly searched and photographed for imaging by utilizing the focusing mode.

With continued reference to fig. 1, the carrying platform 2100 of the carrying mechanism 2000 according to the present application has a plate-like structure, and a fixing component for fixing the sample 300 is disposed on the plate-like structure, and the fixing component may be a buckle or other structure capable of fixing the sample 300, which will not be described herein.

The driving mechanism of the carrying mechanism 2000 provided in the embodiment of the present application may drive the carrying platform 2100 to move along at least one direction, where the at least one direction is a direction parallel to the surface of the single-path optical machine 100 facing the sample 300. Illustratively, the drive mechanism may drive the stage 2100 to move in two directions, with the two directions of movement intersecting so that it may move at any position within a plane parallel to the surface of the single-pass optical machine 100 facing the sample 300, and when moved, different areas on the sample 300 may be adjusted to be under the single-pass optical machine 100 to image the different areas. In a specific arrangement, two directions may be perpendicular to each other.

Illustratively, the drive mechanism may include a first rail 2200 fixed relative to the fixed bracket 200, a base 2300 slidably mounted to the first rail 2200, and a carrier platform 2100 slidably mounted to the base 2300. When the fluorescence microscopy system includes the support 3000, the first guide 2200 may be disposed on the support 3000. The base 2300 is slidably mounted on the first guide 2200 in a first direction, i.e., a longitudinal direction of the first guide 2200. The carrier platform 2100 is slidably mounted on the base 2300, and in particular, a second rail is provided on the base 2300, and the carrier platform 2100 is slidably mounted on the second rail. The carrying platform 2100 may slide on the second rail along a second direction, which is a length direction of the second rail.

When the first direction and the second direction are specifically set, the first direction and the second direction intersect, and may be specifically perpendicular to each other, so that the stage 2100 may move at any position in a plane parallel to the surface of the single-path optical machine 100 facing the sample 300.

In addition, when the first guide rail 2200 is provided, a longitudinal portion of the first guide rail 2200 is located outside the plurality of single-track light machines 100, and when the support base 3000 slides to the first setting position, the loading platform 2100 is located outside the plurality of single-track light machines 100. The length direction of the first rail 2200 of the above example refers to the sliding of the carrier platform 2100 into or out of the collection region of the fluorescence collection mechanism 1000 in the first direction. When the carrying platform 2100 slides out of the collection area (first set position) of the fluorescence collection mechanism 1000, the sample 300 can be put into the carrying platform 2100 at this position; when the carrier platform 2100 slides under the collection area of the fluorescence collection mechanism 1000, fluorescence collection can be performed to achieve detection.

As can be seen from the above description, the fluorescence microscope system provided by the embodiment of the present application adopts 4 single-path light machines 100 to be assembled above the sample 300 in parallel, and the objective lenses 130 of the 4 single-path light machines 100 have the same focal plane. The object carrying platform 2100 drives the sample 300 to scan in different areas of the same plane, after the sample 300 moves to an imaging position, the 4 single-path optical machines 100 are simultaneously started to focus, an optimal focal plane of the objective 130 is found, after the focal plane is found, the photographing imaging procedures of the plurality of single-path optical machines 100 are simultaneously started, and fluorescence microscopic imaging is completed. The plurality of single-path optical machines 100 can simultaneously complete photographing and imaging of a plurality of sample 300 areas, so that fluorescence detection efficiency is greatly improved, cost is reduced, and meanwhile, the scheme that the carrying platform 2100 drives the sample 300 to move for scanning and imaging is adopted, so that the stability and reliability of the single-path optical machine 100 system are improved.

The present disclosure is intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Any omissions, modifications, equivalents, improvements, and the like, which are within the spirit and principles of the one or more embodiments of the disclosure, are therefore intended to be included within the scope of the disclosure.

The foregoing is merely illustrative embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily think about variations or substitutions within the technical scope of the present application, and the application should be covered. Therefore, the protection scope of the application is subject to the protection scope of the claims.

Claims (10)

1. A fluorescence microscopy system, comprising:

the fluorescence acquisition mechanism comprises a fixed bracket and a plurality of single-path optical machines arranged on the fixed bracket in an array manner; the single-path optical machine is used for exciting and collecting fluorescence of the sample, and the collecting ranges of the single-path optical machines are at least partially not overlapped;

the carrying mechanism comprises a carrying platform which is positioned below the plurality of single-path optical machines and used for carrying the samples, and a driving mechanism for driving the carrying platform to move along at least one direction.

2. The fluorescence microscopy system of claim 1, wherein the number of the single-pass light machines is four, and the four single-pass light machines are arranged in a rectangular array.

3. The fluorescence microscopy system of claim 1, wherein each of the single-pass light engines comprises:

an excitation light source assembly for emitting light that excites fluorescence of the sample;

a focusing light source assembly for emitting a first focusing light ray irradiated to the sample;

the objective lens is used for transmitting the light rays emitted by the excitation light source assembly to the sample and receiving fluorescence excited by the sample; and propagating the first focusing light to the sample and receiving a second focusing light reflected by the sample;

imaging means for collecting fluorescence excited by the sample; focusing and imaging according to the second focusing light rays;

the first dichroic mirror is used for reflecting the light rays emitted by the excitation light source component and the first focusing light rays to the objective lens; and transmitting the fluorescence excited by the sample and the second focusing light to the imaging device.

4. The fluorescence microscopy system of claim 3, wherein each of the single-pass light engines further comprises: the first double-bandpass filter is positioned between the first dichroic mirror and the imaging device and is used for filtering illumination light doped in fluorescence excited by the sample and transmitting the fluorescence excited by the filtered sample to the imaging device.

5. The fluorescence microscopy system of claim 4, wherein the single pass light engine further comprises a barrel mirror positioned between the imaging device and the first dual bandpass filter, the barrel mirror having a plurality of lenses disposed therein; the lenses are used for focusing fluorescence excited by the filtered sample to the imaging device through the first double-bandpass filter.

6. The fluorescence microscopy system of claim 3, wherein the excitation light source assembly comprises: a first light source for emitting light of a first wavelength, and a second light source for emitting light of a second wavelength; further comprises:

a second dichroic mirror for transmitting the first wavelength light and reflecting the second wavelength light; and a second dual bandpass filter for filtering fluorescence bands in the first wavelength light and the second wavelength light.

7. The fluorescence microscopy system of claim 6, wherein the focused light source assembly comprises: a laser emission source; an aperture diaphragm located on a propagation light path of the laser emitted by the laser emission source; and a third dichroic mirror for reflecting the laser light emitted from the laser light emitting source and transmitting the first wavelength light and the second wavelength light filtered by the second dual-band pass filter.

8. The fluorescence microscopy system of any of claims 1-7, wherein the drive mechanism comprises: the first guide rail is fixed relative to the fixed support, the base is slidably assembled on the first guide rail, and the carrying platform is slidably assembled on the base; wherein,

the sliding direction of the base is intersected with the sliding direction of the carrying platform; and the sliding direction of the base and the sliding direction of the carrying platform are respectively parallel to the surfaces of the plurality of single-path optical machines facing the sample.

9. The fluorescence microscopy system of claim 8, further comprising a support base, the stationary mount being fixedly connected to the support base;

the first guide rail is fixed on the supporting seat.

10. The fluorescence microscopy system of claim 8, wherein the lengthwise portion of the first rail is located outside the plurality of single-pass light engines and the load platform is located outside the plurality of single-pass light engines when the support base is slid to the first set position.