CN117025393A - Cell-restricted migration micro-fluidic chip and preparation method and application thereof - Google Patents
Cell-restricted migration micro-fluidic chip and preparation method and application thereof Download PDFInfo
- Publication number
- CN117025393A CN117025393A CN202310942139.7A CN202310942139A CN117025393A CN 117025393 A CN117025393 A CN 117025393A CN 202310942139 A CN202310942139 A CN 202310942139A CN 117025393 A CN117025393 A CN 117025393A
- Authority
- CN
- China
- Prior art keywords
- migration
- cell
- limiting
- cell culture
- restricted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005012 migration Effects 0.000 title claims abstract description 112
- 238000013508 migration Methods 0.000 title claims abstract description 112
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 238000004113 cell culture Methods 0.000 claims abstract description 59
- 230000000670 limiting effect Effects 0.000 claims abstract description 47
- 230000002093 peripheral effect Effects 0.000 claims abstract description 14
- 230000012292 cell migration Effects 0.000 claims abstract description 7
- 229940079593 drug Drugs 0.000 claims description 13
- 239000003814 drug Substances 0.000 claims description 13
- 239000000758 substrate Substances 0.000 claims description 7
- 238000013537 high throughput screening Methods 0.000 claims description 5
- 230000008685 targeting Effects 0.000 claims description 5
- 239000002861 polymer material Substances 0.000 claims description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 3
- 238000011161 development Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 229920002120 photoresistant polymer Polymers 0.000 claims description 3
- 238000004080 punching Methods 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 8
- 210000002220 organoid Anatomy 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 57
- 230000008611 intercellular interaction Effects 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 238000003501 co-culture Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000005868 ontogenesis Effects 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- -1 polydimethylsiloxane Polymers 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 206010013710 Drug interaction Diseases 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Abstract
The application discloses a cell restriction migration micro-fluidic chip, a preparation method and application thereof, relates to the technical field of cell migration micro-fluidic chips, and provides a cell restriction migration micro-fluidic chip, wherein the chip is provided with a restriction migration layer and a basal layer from top to bottom; the limiting migration layer consists of an array limiting migration unit; the limiting migration unit consists of a central cell culture cavity, a peripheral cell culture cavity and a cell limiting migration area; the cell-restricted migration zone consists of several microchannels with height and width dimensions smaller than the cell diameter. The application also provides a preparation method of the microfluidic chip. The application can culture one cell/organoid in the central cell culture cavity and one or more cells/organoids in the peripheral cell culture cavity, thus realizing high-throughput research of the influence of cells on cell restricted migration.
Description
Technical Field
The application relates to the technical field of cell migration micro-fluidic chips, in particular to a cell restriction migration micro-fluidic chip, a preparation method and application thereof.
Background
Cell migration is an extremely important cellular process in ontogenesis, injury repair, and tumor metastasis. During the migration of cells in vivo, a heterogeneous microenvironment is passed through, cells and extracellular matrix in the microenvironment form narrow micropores and microchannels, a restricted migration path allowing the cells to pass through is formed, and the cells utilize various migration matrixes to change the shape of the cells through a recombination framework to realize the migration. Regarding the research of cell restricted migration, the method has important significance for deeply understanding the mechanism of ontogenesis and injury repair and developing antitumor drugs targeting cell restricted migration.
Publication (bulletin) No. (CN 115197841A) discloses microfluidic devices for cell culture and drug screening for restricted migration; the chip comprises a first channel and a second channel which is arranged in parallel with the first channel; the first channel is communicated with the second channel through a plurality of restricted migration channels; and the depth of the limiting migration channel is smaller than that of the first channel and the second channel; the two ends of the first channel are provided with a first inlet and a first outlet, and the two ends of the second channel are provided with a second inlet and a second outlet; the method comprises the steps of arranging a plurality of first channels and second channels, and arranging a pyramid shunting structure for shunting the first channels for ensuring smooth shunting; when the cell culture device is used, cells to be cultured are added into one of the first channel and the second channel, and drugs or cells capable of affecting cell restricted migration are added into the other channel; the blocking effect of the drug or the cell on the cell restricted migration is completed, and then the drug and the cell which are effective for the restricted migration are screened. However, this application cannot culture cells in an open structure cell culture chamber, and the culture chambers are not communicated with each other by a restricted migration microchannel, so that the cell inoculation and culture operations are complicated, and furthermore, the application cannot study the influence of cell-cell interaction on the restricted migration under the condition of cell co-culture.
Disclosure of Invention
Aiming at the problems, the application aims to provide a cell restricted migration micro-fluidic chip, a preparation method and application thereof, wherein cell culture cavities are of an open structure, the culture cavities are communicated by a restricted migration micro-pipeline, cell inoculation and culture operations are simple, and in addition, the influence of cell-cell interaction on restricted migration can be studied under the condition of cell co-culture.
The main idea of the technical scheme adopted by the application is as follows: the application relates to a microfluidic chip for high-throughput research of cell-restricted migration mechanism or high-throughput screening of targeted cell-restricted migration drugs based on various cell-cell interactions or cell-various drug interactions and a preparation method thereof. Based on the above, a micro-fluidic chip for limiting cell migration is designed, wherein the chip is provided with a limiting migration layer and a basal layer from top to bottom; the limiting migration layer consists of an array limiting migration unit; the limiting migration unit consists of a central cell culture cavity, a peripheral cell culture cavity and a cell limiting migration area; the peripheral cell culture chamber consists of a plurality of microcavities surrounding the central cell culture chamber; the cell-restricted migration zone consists of several microchannels with height and width dimensions smaller than the cell diameter.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows:
a cell-restricted migration microfluidic chip comprising a substrate layer, characterized in that: a limiting migration layer is arranged on the basal layer;
the limiting migration layer is provided with a plurality of limiting migration units, and each limiting migration unit is internally provided with a cell culture structure;
the cell culture structure is a cell culture cavity, and a cell restricted migration zone is arranged in the cell culture cavity.
Further, the cell culture chamber includes a central cell culture chamber and a plurality of peripheral cell culture chambers located around the central cell culture chamber.
Further, a cell-restricted migration zone is provided between the central cell culture chamber and each of the surrounding cell culture chambers.
Further, a plurality of micro-channels for communicating the central cell culture chamber with the surrounding cell culture chambers are provided in the cell restricted migration zone.
The preparation method of the cell-restricted migration micro-fluidic chip is characterized by comprising the following steps of:
s1: making the limiting migration layer into a photomask;
s2: coating photoresist on a silicon wafer, and carrying out ultraviolet exposure and development to prepare a limiting migration layer chip template;
s3: covering the surface of the limiting migration layer chip template with a high polymer material, and curing;
s4: stripping the solidified limiting migration layer chip from the template, and punching holes at the design position;
s5: and bonding the limiting migration layer chip and the basal layer to prepare the microfluidic chip.
The cell restricted migration microfluidic chip is applied to high-throughput research on cell restricted migration influence and high-throughput screening research on biochemical factors and medicines targeting cell restricted migration.
The beneficial effects of the application are as follows: compared with the prior art, the application has the advantages that,
the application can culture one cell/organoid in the central cell culture cavity and one or more cells/organoids in the peripheral cell culture cavity, thus realizing high-throughput research of the influence of cells on cell restricted migration.
The application can also culture a cell in a central cell culture cavity, and provide one or more biochemical factors/medicines in peripheral cell culture cavities, thereby realizing high-throughput screening of the biochemical factors/medicines for targeted cell restricted migration.
Drawings
FIG. 1 is a schematic diagram of a cell-restricted migration microfluidic chip according to the present application.
Fig. 2 is a bottom view of the chip-limiting transfer unit of the present application.
FIG. 3 is a cross-sectional view of a cell-limiting migration unit of the present application.
Wherein: 1. a limiting migration layer 2, a basal layer 3, a limiting migration unit 4, a central cell culture cavity 5, a peripheral cell culture cavity 6, a cell limiting migration zone 7 and a micro-pipeline.
Detailed Description
In order to enable those skilled in the art to better understand the technical solution of the present application, the technical solution of the present application is further described below with reference to the accompanying drawings and examples.
Referring to fig. 1-3, the application discloses a cell-restricted migration microfluidic chip, which comprises a microfluidic chip, wherein a basal layer 2 is arranged on the surface of the microfluidic chip, and a plurality of migration-restricted layers 1 are adhered to the basal layer 2;
wherein the restrictive migration layer 1 comprises an array-type restrictive migration unit 3; the limiting migration unit 3 comprises a central cell culture chamber 4, a plurality of peripheral cell culture chambers 5 and a plurality of cell limiting migration areas 6; the cell restricted migration zone 6 comprises a plurality of micro-ducts 7, the micro-ducts 7 communicating the central cell culture chamber 4 with the surrounding cell culture chamber 5. Note that, each structure in the limiting transfer unit 3 and the limiting transfer unit 3 are integrally formed.
The material of the chip limiting migration layer 1 and the substrate layer 2 is glass, polydimethylsiloxane or other elastic polymer materials.
More specifically, the cell restriction migration micro-fluidic chip is rectangular, the length L is 12.7mm, the width W is 85.5mm, and the height H is 5-25mm; 2-8 rows of restrictive migration units 3 are arranged on the restrictive migration layer 1, each row comprises 3-12 restrictive migration units 3, each restrictive migration unit 3 is square, and the side length is 7-15mm;
the central cell culture chamber 4 and the peripheral cell culture chamber 5 of the restrictive transfer unit 3 are of an open circular or regular polygonal structure; the cell-restricted migration zone 6 has a height h of 3-20 μm, a width w of 3-1000 μm and a length l of 50-500. Mu.m.
The preparation method of the cell-restricted migration micro-fluidic chip comprises the following steps:
firstly, manufacturing a photomask from a designed limiting migration layer structure;
coating photoresist on a silicon wafer, and carrying out ultraviolet exposure and development to prepare a limiting migration layer chip template;
covering polydimethylsiloxane or other high polymer materials on the surface of the limiting migration layer chip template, and baking and curing;
fourthly, stripping the solidified limiting migration layer chip from the template, and punching holes at the design position;
and fifthly, treating the limiting migration layer chip and the glass substrate layer by using a plasma treatment instrument, and bonding the limiting migration layer chip and the glass substrate layer to prepare the microfluidic chip.
Examples
The cell restricted migration microfluidic chip of the application can be used for high-throughput research of influence of cells on cell restricted migration and high-throughput screening research of biochemical factors/drugs targeting cell restricted migration, and specifically comprises the following steps:
step one, adding a cell suspension into a central cell culture cavity 4 of a chip array type limiting migration unit by using a pipetting gun, and standing a chip in a cell culture box for cell culture or organoid formation culture;
step two, adding other types of cells into the surrounding cell culture cavity 5 according to experimental requirements, and standing the chip in a cell culture box for cell culture or organoid formation culture;
thirdly, observing migration conditions of cells in the micro-pipeline 7 of the cell restricted migration zone 6, and analyzing influence of cell-interaction on cell restricted migration;
step four, adding the required biochemical factors or medicines into the surrounding cell culture cavity 5 according to experimental requirements, and observing the migration condition of cells in the micro-pipeline 7 of the cell restricted migration zone 6;
step five, observing migration conditions of cells in the micro-pipeline 7 of the cell restricted migration zone 6, and analyzing influence of biochemical factors/drugs on the cell restricted migration;
step six, adding cell morphology and function detection reagents into the central cell culture cavity 4 and the peripheral cell culture cavity 5 for cell morphology and function detection after cell-cell interaction or biochemical factors/medicines are treated on cells; or adding cell lysate into the central cell culture chamber 4 and the peripheral cell culture chamber 5, extracting cell components, detecting gene and protein expression of cells on the outside of the chip, analyzing the influence of the cells on cell restricted migration based on the cell lysate, and targeting the biochemical factors/drugs of cell restricted migration.
The foregoing has shown and described the basic principles, principal features and advantages of the application. It will be understood by those skilled in the art that the present application is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present application, and various changes and modifications may be made without departing from the spirit and scope of the application, which is defined in the appended claims. The scope of the application is defined by the appended claims and equivalents thereof.
Claims (6)
1. A cell-restricted migration microfluidic chip comprising a substrate layer (2), characterized in that: a limiting migration layer (1) is arranged on the substrate layer (2);
a plurality of limiting migration units (3) are arranged on the limiting migration layer (1), and a cell culture structure is arranged in each limiting migration unit (3);
the cell culture structure is a cell culture cavity, and a cell restricted migration zone is arranged in the cell culture cavity.
2. The cell-restricted migration microfluidic chip of claim 1, wherein: the cell culture chamber comprises a central cell culture chamber (4) and a plurality of peripheral cell culture chambers (5) located around the central cell culture chamber (4).
3. The cell-restricted migration microfluidic chip of claim 2, wherein: a cell restricted migration zone (6) is arranged between the central cell culture chamber (4) and each peripheral cell culture chamber (5).
4. A cell-restricted migration microfluidic chip according to claim 3, wherein: the cell restricted migration zone (6) is provided with a plurality of micro-channels (7) for communicating the central cell culture chamber (4) with the surrounding cell culture chambers (5).
5. The method for preparing a cell-restricted migration micro fluidic chip according to any one of claims 1 to 4, comprising the steps of:
s1: forming a limiting migration layer (1) into a photomask;
s2: coating photoresist on a silicon wafer, and carrying out ultraviolet exposure and development to prepare a limiting migration layer chip template;
s3: covering the surface of the limiting migration layer chip template with a high polymer material, and curing;
s4: stripping the solidified limiting migration layer chip from the template, and punching holes at the design position;
s5: and bonding the limiting migration layer chip and the substrate layer (2) to prepare the microfluidic chip.
6. A cell migration limiting microfluidic chip according to any one of claims 1-4 for use in high throughput studies of cell migration limiting effects, and in high throughput screening studies of biochemical factors, drugs targeting cell migration limiting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310942139.7A CN117025393A (en) | 2023-07-29 | 2023-07-29 | Cell-restricted migration micro-fluidic chip and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310942139.7A CN117025393A (en) | 2023-07-29 | 2023-07-29 | Cell-restricted migration micro-fluidic chip and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117025393A true CN117025393A (en) | 2023-11-10 |
Family
ID=88640513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310942139.7A Pending CN117025393A (en) | 2023-07-29 | 2023-07-29 | Cell-restricted migration micro-fluidic chip and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117025393A (en) |
-
2023
- 2023-07-29 CN CN202310942139.7A patent/CN117025393A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1601874B1 (en) | Integrated microfluidic control employing programmable tactile actuators | |
| CN101948741B (en) | Microfluidic gene chip for nucleic acid sequencing | |
| Sugiura et al. | Pressure‐driven perfusion culture microchamber array for a parallel drug cytotoxicity assay | |
| EP2646798B1 (en) | Microanalysis of cellular function | |
| JP7257565B2 (en) | Microfluidic device, method of manufacture, and use thereof | |
| Gao et al. | A microfluidic localized, multiple cell culture array using vacuum actuated cell seeding: integrated anticancer drug testing | |
| CN107881106B (en) | Array type cell dynamic culture and regionalization processing micro-fluidic chip and preparation method and application thereof | |
| CN104561286B (en) | A kind of novel polymeric enzyme chain reaction micro-fluid chip control system and preparation method thereof | |
| WO2017164636A1 (en) | Fine particle manufacturing device | |
| CN111621420B (en) | Cell co-culture micro-fluidic chip for enhancing neuron function | |
| KR101201939B1 (en) | Microfluidic platform and preparation method of the same | |
| US20160136642A1 (en) | A Microfluidic Device with Pillars | |
| CN117025393A (en) | Cell-restricted migration micro-fluidic chip and preparation method and application thereof | |
| Schudel et al. | Microfluidic platforms for RNA interference screening of virus–host interactions | |
| CN108384713A (en) | A kind of cell migration micro-fluidic chip and preparation method thereof | |
| CN111909838A (en) | Micro-fluidic chip for cell function detection | |
| KR20110064549A (en) | A microfluidic device for cultivating cells, and a method for cultivating cells using the same | |
| US20210339242A1 (en) | Microfluidic device with embedded cell culture chambers for high throughput biological assays | |
| CN111019829B (en) | Single cell array chip and its preparation method and use | |
| JP7246041B2 (en) | CELL CULTURE CHIP AND MANUFACTURING METHOD THEREOF | |
| KR101223274B1 (en) | Cell trapping apparatus | |
| Cheng et al. | An open-chamber flow-focusing device for focal stimulation of micropatterned cells | |
| CN114100714B (en) | Nucleic acid or polypeptide high-throughput synthesis chip and application thereof | |
| KR102359971B1 (en) | Microfluidic chip for the production of useful materials of microorganisms | |
| Li | Micromachining of Polymeric Microfluidic Micro/Nanoelectroporation Device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |