CN117019246A - Sequential micromixer based on sine wave vortex flow and working method - Google Patents
Sequential micromixer based on sine wave vortex flow and working method Download PDFInfo
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- CN117019246A CN117019246A CN202311004131.2A CN202311004131A CN117019246A CN 117019246 A CN117019246 A CN 117019246A CN 202311004131 A CN202311004131 A CN 202311004131A CN 117019246 A CN117019246 A CN 117019246A
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- 238000000034 method Methods 0.000 title claims description 25
- 238000002156 mixing Methods 0.000 claims abstract description 38
- 239000000725 suspension Substances 0.000 claims abstract description 36
- 239000012530 fluid Substances 0.000 claims abstract description 26
- 230000005684 electric field Effects 0.000 claims abstract description 13
- 239000011521 glass Substances 0.000 claims abstract description 10
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 8
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 8
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract description 8
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims description 42
- 239000007853 buffer solution Substances 0.000 claims description 24
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 24
- 239000008346 aqueous phase Substances 0.000 claims description 15
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 8
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 239000013543 active substance Substances 0.000 claims description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 229920000136 polysorbate Polymers 0.000 claims description 4
- 235000011164 potassium chloride Nutrition 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000012071 phase Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
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- 238000006243 chemical reaction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000005370 electroosmosis Methods 0.000 description 3
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- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000004113 cell culture Methods 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/3031—Micromixers using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- Organic Chemistry (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a sequential micromixer based on sine wave vortex flow, comprising: a glass substrate; the PDMS cover plate is provided with a micro-channel and is arranged on the glass substrate; 2 3D electrodes respectively arranged on two sides of the micro-channel; one end of the planar electrode is connected with the 3D electrode, and the other end of the planar electrode is externally connected with an external power supply so as to form a uniform electric field in the micro-channel through the 3D electrode; and the 2 suspension electrodes are arranged in the micro-channel and are sequentially and asymmetrically arranged along the fluid flow direction of the micro-channel. The sequential micromixer of the present invention has efficient and controllable mixing effects.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a sequential micromixer based on sine wave vortex flow and a working method thereof.
Background
Microfluidic chip technology (LOC for short) has been developed robustly in the fields of chemistry, biology, material analysis, etc., and plays an important role in laboratory applications. Conventional experimental methods often rely on culture dishes or cell culture flasks, but these methods have some limitations, such as difficulty in batch processing, difficulty in simulating the in vivo conditions of the cell growth environment, and the like. To overcome these limitations and improve experimental efficiency and accuracy, microfluidic technology has evolved.
Among them, the micromixing technology is one of important research directions in the field of microfluidics, and aims to rapidly contact molecules from different reagents within a microchannel, thereby achieving efficient reaction and analysis. In particular for multicomponent reactions, organic synthesis and highly complex biomacromolecule detection, multiplex micromixing is critical for successful experiments. However, in past studies, there has been little research on sequential mixing of parallel streams, mainly focused on two-fluid mixing in a single channel, or on segmented channel mixing of multiple fluids.
Conventional sequential mixing methods typically require multiple steps of mechanical agitation, which not only risks reagent consumption and non-uniformity in concentration, but also for rapid reactions or quantitative detection, precise control of reactant usage is not possible due to insufficient mixing. To overcome the limitations of conventional mixing, fluid mixing within passive and active microchannels has attracted considerable attention and has proven to be effective in enhancing mass transfer. Passive micromixing typically relies on specific geometries or complex microchannel structures embedded in the microchannels. In addition to complex manufacturing processes, such methods are commonly used to achieve multi-step or simultaneous micromixing in three or more fluids. Challenges remain in the multi-fluid parallel mixing aspect. In contrast, active micromixing is driven by external energy sources, such as magnetic fields, acoustic energy, electrical stimulation, and optical fields. The active mixer can perform micromixing rapidly and uniformly in a simple channel and can be completed in a short time. More importantly, the mixing position and mixing time can be conveniently controlled by the design achieved by the external stimulus.
Disclosure of Invention
The technical idea of the invention is as follows: in the active micromixer, electrodynamic methods such as inductive electroosmotic flow (ICEO) and alternating-current electrothermal coupling (ACET) have the characteristics of simple electrode structure, no moving parts and low voltage, making them attractive hybrid mechanisms in various biological analysis applications. Interestingly, ICEO is created on the electrically floating electrode, which by inducing a voltage rather than directly applying an electrical signal, micro-eddy currents that exhibit ICEO basis can occur at any desired location. It appears that the ICEO flow value is expected to meet the multi-fluid micromixing requirements.
The invention provides a sequential micromixer based on sine wave vortex flow and a working method thereof, which adopt the sine wave vortex flow principle and realize sequential micromixer by utilizing reconfigurable ICEO-based micro vortex.
An aspect of embodiments of the present specification discloses a sequential micromixer based on sinusoidal wave swirl flow, comprising:
a glass substrate;
the PDMS cover plate is provided with a micro-channel and is arranged on the glass substrate;
2 3D electrodes respectively arranged on two sides of the micro-channel;
one end of the planar electrode is connected with the 3D electrode, and the other end of the planar electrode is externally connected with an external power supply so as to form a uniform electric field in the micro-channel through the 3D electrode;
and the 2 suspension electrodes are arranged in the micro-channel and are sequentially and asymmetrically arranged along the fluid flow direction of the micro-channel.
In one embodiment disclosed in the specification, the 2 suspension electrodes are rectangular suspension electrodes and sinusoidal suspension electrodes in sequence along the fluid flow direction of the microchannel.
In one embodiment disclosed in the specification, the micro-channel comprises a first channel, a second channel, a third channel and a mixing channel which are communicated, wherein the first channel is provided with a first water phase inlet, the second channel is provided with a second water phase inlet, the third channel is provided with a third water phase inlet, the mixing channel is provided with an outlet, and the first water phase inlet, the second water phase inlet, the third water phase inlet and the outlet are all arranged on the PDMS cover plate.
In one embodiment disclosed in the present specification, 2 3D electrodes are respectively disposed at two sides of the mixing channel.
In one embodiment disclosed in the present specification, the rectangular suspension electrode and the sinusoidal suspension electrode are disposed within the mixing channel.
Another aspect of the embodiments of the present disclosure discloses a method for operating a sequential micromixer based on sine wave swirl flow, implemented by the sequential micromixer based on sine wave swirl flow described above;
the working method of the sequential micromixer based on sine wave vortex flow comprises the following steps of;
injecting a solution to be mixed into the microchannel;
and an external power supply is turned on, and a uniform electric field is formed in the micro-channel through the 3D electrode so as to induce an electric double layer through the suspension electrode, and then an asymmetric fluid vortex is formed in the micro-channel, so that rapid micro-mixing of different fluids is realized.
In one embodiment disclosed in the present specification, the method for operating a sequential micromixer based on sine wave swirl flow further comprises:
buffer solution was prepared: preparing a buffer solution with the pH value of 9.2 and the conductivity of 0.2S/m by adding potassium chloride and ammonia water;
preparing a fluorescein solution with the concentration of 1.32X10-5 mol/L;
9: mixing absolute ethyl alcohol and tween solution according to a volume ratio of 1 to prepare an active agent solution;
1, the method comprises the following steps: 99 to the buffer and the fluorescein solution, respectively, to complete the preparation of the solution to be mixed.
In one embodiment disclosed in the present specification, the method for operating a sequential micromixer based on sine wave swirl flow further comprises:
respectively installing the syringes filled with the buffer solution and the fluorescein solution on three syringe pumps, wherein the buffer solution syringe pumps are installed at inlets on two sides of the micro-channel, and the fluorescein solution syringe pumps are installed at inlets in the middle of the micro-channel;
opening a buffer solution injection pump, and injecting buffer solution into the micro-channel to soak the channel;
and (3) opening a fluorescein solution injection pump to inject the fluorescein solution into the microchannel.
The embodiment of the specification can at least realize the following beneficial effects:
the invention forms a uniform electric field in the micro-channel through the planar electrode and the 2 3D electrodes; through 2 suspension electrodes, asymmetric fluid eddies are formed in the micro-channel, so that rapid micro-mixing of different fluids is promoted, and the efficient and controllable mixing effect is achieved.
The invention promotes the rapid mixing of different reagents in the fluid, is suitable for chemical reaction, biological experiments and other laboratory applications, and provides a new driving force for the application and development of the microfluidics technology in the biomedical field. Compared with the traditional sequential mixer, the invention has simpler structure and higher mixing efficiency, provides a more convenient operation mode for the experimental process, provides a new solution for efficient reaction and sample analysis, and can provide a very promising LOC alternative method for more complex chemical and biological analysis.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic perspective view of a sequential micromixer based on sinusoidal vortex flow in accordance with some embodiments of the present invention.
Fig. 2 is an exploded view of fig. 1.
Fig. 3 is a schematic diagram of the internal structure of a sequential micromixer based on sinusoidal vortex flow according to some embodiments of the present invention.
Fig. 4 is a schematic diagram of the internal dimensional structure of a sequential micromixer based on sinusoidal vortex flow according to some embodiments of the present invention.
FIG. 5 is a schematic cross-sectional dimensional structure of sequential micromixers based on sinusoidal vortex flow in accordance with some embodiments of the present invention.
Reference numerals:
1. a PDMS cover plate; 2. a first aqueous phase inlet; 3. an outlet; 4. a planar electrode; 5. a second aqueous phase inlet; 6. a third aqueous phase inlet; 7. a glass substrate; 8. a 3D electrode; 9. a suspension electrode; 91. rectangular suspension electrodes; 92. a sinusoidal suspension electrode; 10. a first channel; 11. a second channel; 12. a third channel; 13. a mixing channel.
Detailed Description
Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those of skill in the pertinent art, the described embodiments may be modified in various different ways without departing from the spirit or scope of the present invention. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, or orientations or positional relationships conventionally placed in use of the product of the present invention, or orientations or positional relationships conventionally understood by those skilled in the art, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.
The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Furthermore, the terms "mounted," "connected," "secured," and the like are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally formed; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
As shown in fig. 1 and 2, an aspect of the embodiments of the present specification discloses a sequential micromixer based on sine wave swirl flow, comprising:
a glass substrate 7;
a PDMS cover 1 with micro-channels arranged on the glass substrate 7;
2 3D electrodes 8 respectively arranged on two sides of the micro-channel;
one end of the planar electrode 4 is connected with the 3D electrode 8, and the other end of the planar electrode is externally connected with an external power supply so as to form a uniform electric field in the micro-channel through the 3D electrode 8;
and the 2 suspension electrodes 9 are all arranged in the micro-channel and are sequentially and asymmetrically arranged along the fluid flow direction of the micro-channel.
In some embodiments, 2 of the suspension electrodes 9 are rectangular suspension electrodes 91 and sinusoidal suspension electrodes 92 in sequence along the fluid flow direction of the microchannel. The upper and lower surfaces of the sinusoidal suspension electrode 92 are sinusoidally formed by a sinusoid.
In some embodiments, the microchannel comprises a first channel, a second channel, a third channel and a mixing channel in communication, the first channel having a first aqueous phase inlet 2, the second channel having a second aqueous phase inlet 5, the third channel having a third aqueous phase inlet 6, the mixing channel having an outlet 3, the first aqueous phase inlet 2, the second aqueous phase inlet 5, the third aqueous phase inlet 6 and the outlet 3 being all provided on the PDMS cover plate 1.
In some embodiments, 2 3D electrodes 8 are provided on each side of the mixing channel.
In some embodiments, the rectangular suspension electrode 91 and sinusoidal suspension electrode 92 are disposed within the mixing channel.
Another aspect of the embodiments of the present disclosure discloses a method for operating a sequential micromixer based on sine wave swirl flow, implemented by the sequential micromixer based on sine wave swirl flow described above;
the working method of the sequential micromixer based on sine wave vortex flow comprises the following steps of;
injecting a solution to be mixed into the microchannel;
and an external power supply is turned on, a uniform electric field is formed in the micro-channel through the 3D electrode 8, so that an electric double layer is induced through the suspension electrode 9, and then an asymmetric fluid vortex is formed in the micro-channel, and rapid micro-mixing of different fluids is realized.
In some embodiments, the method of operating a sequential micromixer based on sine wave swirl flow further comprises:
buffer solution was prepared: preparing a buffer solution with the pH value of 9.2 and the conductivity of 0.2S/m by adding potassium chloride and ammonia water;
preparing a fluorescein solution with the concentration of 1.32X10-5 mol/L;
9: mixing absolute ethyl alcohol and tween solution according to a volume ratio of 1 to prepare an active agent solution;
1, the method comprises the following steps: 99 to the buffer and the fluorescein solution, respectively, to complete the preparation of the solution to be mixed.
In some embodiments, the method of operating a sequential micromixer based on sine wave swirl flow further comprises:
respectively installing the syringes filled with the buffer solution and the fluorescein solution on three syringe pumps, wherein the buffer solution syringe pumps are installed at inlets on two sides of the micro-channel, and the fluorescein solution syringe pumps are installed at inlets in the middle of the micro-channel;
opening a buffer solution injection pump, and injecting buffer solution into the micro-channel to soak the channel;
and (3) opening a fluorescein solution injection pump to inject the fluorescein solution into the microchannel.
The principle of the invention is as follows:
the outside of the channel is connected with an external power supply by using the plane electrode 4 so as to be connected with the 3D electrodes 8 at the two sides of the channel, and a uniform electric field is formed in the channel; in the internal structure of the micromixer, a rectangular suspension electrode 91 (RFE) and a sinusoidal suspension electrode 92 (SSFE) are asymmetrically arranged in the channel, an alternating electric field can induce an electric double layer in the vicinity of the suspension electrode 9, induced charge electroosmosis (ico) and alternating electric thermal coupling (ACET) and other electromotive forces are generated by the suspension electrode 9 under the excitation electric field, the surface potential of the suspension electrode 9 changes, the electric charge changes, the capacitance of the electric double layer is influenced, and the electroosmosis current on the surface of the suspension electrode 9 is changed, so that asymmetric fluid vortex is formed in the channel. Meanwhile, the position of the suspension electrode 9 has a remarkable influence on the electric field, the formation of an electric double layer is controlled, and once the position of the suspension electrode 9 is changed asymmetrically along the width of a channel, the electric field and the electric double layer are rebuilt, and the phenomenon can cause time-varying micro-fluid vortexes corresponding to the shape of the suspension electrode 9 and alternately interfere with contact interfaces of left and right fluids, so that rapid micro-mixing between different fluids is caused.
In one embodiment, the dimensions of the sequential micromixer based on sinusoidal vortex flow may be as shown in fig. 3-5, or may be set according to practical requirements. As shown in fig. 5, the glass substrate 7 has a height (thickness) of 1.1mm, the pdms cover plate has a height (thickness) of 4mm to 1.1 mm=2.9 mm, and the respective channels (first channel 10, second channel 11, third channel 12, mixing channel 13) have a height of 0.8mm.
1. Experiment preparation: (1) Before the experiment, preparing a buffer solution, namely preparing the buffer solution with the pH value of 9.2 and the conductivity of 0.2S/m by adding potassium chloride and ammonia water; (2) Preparing a fluorescein solution with the concentration of 1.32X10-5 mol/L; (3) at 9: mixing absolute ethyl alcohol and tween solution according to a volume ratio of 1 to prepare an active agent solution; (4) at 1:99 volume ratio the active agent solution was added to the buffer and fluorescein solutions.
2. Experimental operation: (1) Placing the microfluidic chip inserted with the Teflon plastic tube on a microscope stage to facilitate subsequent experimental observation and debugging; (2) The syringes filled with the buffer solution and the fluorescein solution are respectively arranged on three syringe pumps and connected with respective inlet positions on the chip (the fluorescein solution is arranged in the middle and the buffer solution is arranged at two sides); (3) Opening a buffer solution injection pump, injecting buffer solution into the chip, and soaking the channel for 10 minutes; (4) Opening a fluorescein solution injection pump, adjusting to a proper flow rate, and injecting the fluorescein solution into the chip; (5) Turning on a power supply of the signal generator, and adjusting parameters such as voltage, frequency and the like of an external electric signal; (6) Observing and recording the mixing condition of the solution in the chip channel under a microscope; (7) Parameters such as voltage, frequency and flow rate are adjusted and the experimental steps are repeated.
In summary, a plurality of specific embodiments of the present invention are disclosed, and under the condition of no paradox, each embodiment may be freely combined to form a new embodiment, that is, embodiments belonging to alternative schemes may be freely replaced, but cannot be mutually combined; embodiments not belonging to the alternatives can be combined with each other, and these new embodiments also belong to the essential content of the invention.
While the above examples describe various embodiments of the present invention, those skilled in the art will appreciate that various changes and modifications can be made to these embodiments without departing from the spirit and scope of the present invention, and that such changes and modifications fall within the scope of the present invention.
Claims (8)
1. A sequential micromixer based on sinusoidal wave swirl flow, comprising:
a glass substrate;
the PDMS cover plate is provided with a micro-channel and is arranged on the glass substrate;
2 3D electrodes respectively arranged on two sides of the micro-channel;
one end of the planar electrode is connected with the 3D electrode, and the other end of the planar electrode is externally connected with an external power supply so as to form a uniform electric field in the micro-channel through the 3D electrode;
and the 2 suspension electrodes are arranged in the micro-channel and are sequentially and asymmetrically arranged along the fluid flow direction of the micro-channel.
2. The sequential micromixer based on sinusoidal vortex flow according to claim 1, wherein 2 of the suspension electrodes are rectangular suspension electrodes and sinusoidal suspension electrodes in sequence along the fluid flow direction of the microchannel.
3. The sequential micromixer based on sinusoidal vortex flow of claim 2, wherein the microchannel comprises a first channel, a second channel, a third channel and a mixing channel in communication, the first channel having a first aqueous phase inlet, the second channel having a second aqueous phase inlet, the third channel having a third aqueous phase inlet, the mixing channel having an outlet, the first aqueous phase inlet, the second aqueous phase inlet, the third aqueous phase inlet and the outlet all being provided on the PDMS cover plate.
4. A sequential micromixer based on sinusoidal vortex flow according to claim 3, wherein 2 of the 3D electrodes are provided on either side of the mixing channel.
5. The sinusoidal vortex flow based sequential micromixer of claim 4, wherein the rectangular suspension electrode and sinusoidal suspension electrode are disposed within the mixing channel.
6. A method of operating a sequential micromixer based on sinusoidal vortex flow, characterized in that it is achieved by a sequential micromixer based on sinusoidal vortex flow according to any of claims 1 to 5;
the working method of the sequential micromixer based on sine wave vortex flow comprises the following steps of;
injecting a solution to be mixed into the microchannel;
and an external power supply is turned on, and a uniform electric field is formed in the micro-channel through the 3D electrode so as to induce an electric double layer through the suspension electrode, and then an asymmetric fluid vortex is formed in the micro-channel, so that rapid micro-mixing of different fluids is realized.
7. The method of operating a sequential micromixer based on sinusoidal vortex flow of claim 6, further comprising:
buffer solution was prepared: preparing a buffer solution with the pH value of 9.2 and the conductivity of 0.2S/m by adding potassium chloride and ammonia water;
preparing a fluorescein solution with the concentration of 1.32X10-5 mol/L;
9: mixing absolute ethyl alcohol and tween solution according to a volume ratio of 1 to prepare an active agent solution;
1, the method comprises the following steps: 99 to the buffer and the fluorescein solution, respectively, to complete the preparation of the solution to be mixed.
8. The method of operating a sequential micromixer based on sinusoidal vortex flow of claim 6, further comprising:
respectively installing the syringes filled with the buffer solution and the fluorescein solution on three syringe pumps, wherein the buffer solution syringe pumps are installed at inlets on two sides of the micro-channel, and the fluorescein solution syringe pumps are installed at inlets in the middle of the micro-channel;
opening a buffer solution injection pump, and injecting buffer solution into the micro-channel to soak the channel;
and (3) opening a fluorescein solution injection pump to inject the fluorescein solution into the microchannel.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311004131.2A CN117019246A (en) | 2023-08-10 | 2023-08-10 | Sequential micromixer based on sine wave vortex flow and working method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202311004131.2A CN117019246A (en) | 2023-08-10 | 2023-08-10 | Sequential micromixer based on sine wave vortex flow and working method |
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| CN202311004131.2A Pending CN117019246A (en) | 2023-08-10 | 2023-08-10 | Sequential micromixer based on sine wave vortex flow and working method |
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