CN1170147C - Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method - Google Patents
Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method Download PDFInfo
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- CN1170147C CN1170147C CNB01133682XA CN01133682A CN1170147C CN 1170147 C CN1170147 C CN 1170147C CN B01133682X A CNB01133682X A CN B01133682XA CN 01133682 A CN01133682 A CN 01133682A CN 1170147 C CN1170147 C CN 1170147C
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Abstract
The present invention relates to a quick urine iodine quantitative detection reagent kit and a urine iodine detection method. A reagent kit is composed of a digestion solution, an analysis agent, a neutralizing agent, a reducing agent, an indicator, an oxidizing agent and an iodine series standard solution. N detection, firstly, the digestion solution prepared by that overoxidation acyl chloride, sodium hypochlorite and chlorine dioxide are mixed proportionally is used for digesting a urine sample under normal temperature, substances which interfere in the measurement of iodine in urine are decomposed, the urine analysis agent and the acid neutralizing agent are used for decomposing residual digesting liquid and regulating acidity, the end point of reaction is indicated by selecting the indicator (Ferroin) through the reaction of an iodine catalysis and reducing agent (arsenous acid) and the oxidizing agent (sulfuric acid ceria or sulfuric acid ceria ammonium), and reaction time is recorded by a timer. The logarithm value of reaction time (S) is in linear quantitative relation with the logarithm value of the content (C) of iodine, namely that lnC =a+blnS or lgC =a + blgS, and thereby, the content of iodine in urine is calculated. The reagent kit has the advantages of quick detection, accuracy and high sensitivity.
Description
Technical field
The invention belongs to the detectable class, belong to detectable and the detection method of measuring content of iodine in the urine particularly.
Background technology
Urine iodide level is an important indicator of estimating human body iodine nutrition condition.Can diagnose whether iodine deficiency of human body by detecting the urine content of iodine.
Content of iodine is very little in the urine, has only for 1,000,000/zero point several; The urine complicated component, the material that disturbs iodine to measure is a lot.Therefore, measure urine iodine and generally adopt eliminating interfering material earlier, utilize the character of several chemical reactions of catalysis of iodine then, indirect determination urine iodine.
Merck KGaA company proposed urine iodine half-quantitative detection kit in 1998, and this kit adopts the resin filter urine sample, and the organism that disturbs iodine to measure is adsorbed on the resin, got filtrate mensuration.Assay method adopts the reaction of catalysis of iodine tetramethyl biphenyl diamines and hydrogen peroxide, according to hydrogen peroxide the tetramethyl biphenyl diamines is oxidized to the tetramethyl biphenyl diquinone and manifests yellow, relatively more yellow depth degree comes sxemiquantitative to judge that urine iodine contains the iodine scope, detect to be divided into＜100 μ g/L, 100-300 μ g/L and＞300 μ g/L third gear.
Summary of the invention
The objective of the invention is according to above-mentioned present situation condition, aim to provide a kind of urine iodine detection by quantitative kit and urine iodine detecting method that can fast, accurately detect content of iodine in the urine.
The implementation of the object of the invention is, urine iodine detection by quantitative kit, and kit is become to be grouped into by following main agents,
(1) digestion solution: form or wherein single component is formed by peroxidating acyl chlorides, sodium hypochlorite, chlorine dioxide are compound, its total available chlorine content is with Cl
2Meter is at 0.3～1.2mol/L,
(2) resolve agent, be urea solution, its concentration is 0.1～1mol/L in urea,
(3) neutralizing agent: sulfuric acid, hydrochloric acid or phosphoric acid solution, its acidity is counted 1～10mol/L with pH,
(4) reductive agent: be dissolved in the arsenic chloride solution that is prepared in the acid solution by arsenic trioxide, its content is 1 * 10 in arsenic trichloride
-3～0.5mol/L,
(5) indicator: Ferroin solution, its concentration are 5.0 * 10 in Ferroin
-3～1.0 * 10
-2Mol/L,
(6) oxygenant: Cericammoniumsulfate or cerous sulfate solution, content are 5 * 10
-4～5 * 10
-2Mol/L.
Above-mentioned urine iodine detection by quantitative kit detects the method for urine iodine, and concrete steps are:
(1) clear up the serial iodine standard solution that accurately pipettes 50,100,200,300 and 400 μ g/L and each 0.1-1ml of urine sample in test tube, respectively add the 0.2-2ml digestion solution, room temperature leaves standstill after 5 minutes violent jolting test tube repeatedly, and the gas that causes reaction to produce is mainly N
2, O
2With a small amount of Cl
2Till draining, clearing up overall process needs 10-15 minute;
(2) resolve every pipe and add 0.2-2ml and resolve agent, room temperature leaves standstill that violent jolting test tube repeatedly drains the gas that reaction produces after 2 minutes, and overall process must not be less than 10 minutes;
(3) the every pipe of neutralization adds the 0.2-2ml neutralizing agent, and violent jolting test tube repeatedly drains the gas that reaction produces, and overall process must not be less than 10 minutes;
(4) sample handled well was placed 10 minutes at the mensuration place, treat temperature balance after every pipe add the 0.2-2ml reductive agent, shake up;
(5) measure every pipe and add the 0.2-2ml indicator, shake up, a selected pipe, add the 0.2-2ml oxygenant fast with sample injector on the other hand, another hand starts manual time-keeping simultaneously, and takes out this test tube immediately and shake up, and this moment, test solution became blueness, carrying out along with reaction, test solution will become bluish violet from blueness, become redness at last, stop stopwatch when test solution becomes bluish violet and just redness occurred, recording reacting time
Repeat above operation, measure and write down all the other sample hose reaction time;
(6) calculating of urine content of iodine
Adopt the linear regression equation method to calculate the urine content of iodine, because of logarithm value lnC or the lgC of the logarithm value lnS of reaction time S or lgS and iodine concentration C (μ g/L) linear, obtain the regression equation of typical curve by two recurrence statistical methods, lnC=a+blnS or lgC=a+blgS, a wherein, b is respectively the intercept and the slope of curve, be constant, try to achieve by returning statistical method, it is different constants under different temperatures, regression equation with the reaction time substitution typical curve of sample hose calculates the sample content of iodine.
The present invention with digestion solution (peroxidating acyl chlorides, sodium hypochlorite, chlorine dioxide or potpourri wherein) in vitro decompose urine in organism get rid of interfering material; Pass through to resolve the digestion solution of agent decomposing excessive again and regulate acidity; Reconcile acidity by neutralizing agent, and further decompose remaining digestion solution; By catalysis of iodine arsenious acid and cerous sulfate reaction, use indicator Ferroin Indicator Reaction terminal point then, the record reaction is from beginning to the needed time of terminal point.
The present invention in detection, the first pre-treatment process that iodine standard solution, urine sample are cleared up, resolve, neutralized, the suggestion of these processes is carried out in outdoor ventilation place or fuming cupboard, to avoid the suction of irritative gas.The sample handled well was placed 10 minutes at the mensuration place, treat temperature balance after, every pipe adds the 0.2-2ml reductive agent, shakes up.
During mensuration, every pipe adds the 0.2-2ml indicator, shakes up.Because of the easy passivation of indicator overlong time in solution, once the pipe number of Jia Ruing too much, is not advisable with 20 pipes.Be ready to stopwatch, minute book and a blank sheet of paper, below carry out the single tube operation.A selected pipe adds the 0.2-2ml oxygenant fast with sample injector on the other hand, and another hand starts manual time-keeping simultaneously, and takes out this test tube immediately and shake up, and test solution becomes blueness.With the blank sheet of paper is background, observes change in color in vitro.Along with the carrying out of reaction, test solution will become bluish violet from blueness, become redness at last.When test solution becomes bluish violet and redness just occurred, stop stopwatch during catastrophe point, recording reacting time.Catastrophe point normal root really needs only consistent according to operator's observation habit.
The calculating of urine content of iodine adopts the linear regression equation method to calculate the urine content of iodine.Because of logarithm value lnC or the lgC of the numerical value lnS of reaction time S or lgS and iodine concentration C linear, with the logarithm of the concentration of standard solution logarithm input function counter in corresponding its reaction time, obtain the regression equation of typical curve, lnC=a+blnS or lgC=a+blgS by two recurrence statistical methods.A, b are respectively the intercept and the slopes of curve in the formula, are different constants under different temperatures.With the regression equation of the reaction time substitution typical curve of sample hose, calculation sample content of iodine.The sensitivity of this kit is 5 μ g/l.
Testing process of the present invention can be simplified, and resolving agent and neutralizing agent can selectivity add, and promptly iodine standard solution, urine sample add earlier and resolve agent or neutralizing agent after clearing up, and add reductive agent again and reduce.Neutralizing agent, reductive agent, indicator be three or wherein the two mixes the back and adds in advance, neutralizes simultaneously, reduces, indication process or two processes wherein.
Embodiment
Below enumerate embodiments of the invention:
Reagent composition and content
Digestion solution: available chlorine content is 0.6mol/l; Resolve agent: urea content is 0.25mol/l; Neutralizing agent: sulfuric acid concentration is 2.5mol/L; Reductive agent: arsenic trioxide content is 6.0 * 10
-2Mol/L; Indicator: Ferroin (ferrous spirit) content is 3.6 * 10
-2Mol/L; Oxygenant: the content of Cericammoniumsulfate is 7.0 * 10
-3Mol/L.
Testing result
Detect by detection method of the present invention.Wherein, standard solution and urine sample are respectively got 0.2ml, and the consumption of other 6 kinds of reagent of every pipe is 0.5ml, and it is as follows to record one group of data under 17 ℃ of room temperatures:
Standard and sample (μ g/L) 50 100 200 300 400 samples 1 sample 2
Reaction time (S) 78.03 50.91 31.43 25.24 20. 45.96 32.32
The regression equation of trying to achieve typical curve with function calculator is:
lnc＝10.759-1.571lns
With sample 1 and sample 2 reaction time substitution regression equation, the content of iodine that draws sample 1 is 115.1 μ g/L, and the content of iodine of sample 2 is 200.1 μ g/L.
Claims (4)
1, urine iodine detection by quantitative kit is characterized in that kit is become to be grouped into by following main agents,
(1) digestion solution: form or wherein single component is formed by peroxidating acyl chlorides, sodium hypochlorite, chlorine dioxide are compound, its total available chlorine content is with Cl
2Meter is at 0.3～1.2mol/L,
(2) resolve agent, be urea solution, its concentration is 0.1～1mol/L in urea,
(3) neutralizing agent: sulfuric acid, hydrochloric acid or phosphoric acid solution, its acidity is counted 1～10mol/L with pH,
(4) reductive agent: be dissolved in the arsenic chloride solution that is prepared in the acid solution by arsenic trioxide, its content counts 1 * 10 with arsenic trichloride
-3～0.5mol/L,
(5) indicator: Ferroin solution, its concentration are 5.0 * 10 in Ferroin
-3～1.0 * 10
-2Mol/L,
(6) oxygenant: Cericammoniumsulfate or cerous sulfate solution, content are 5 * 10
-4～5 * 10
-2Mol/L.
2, a kind of method of utilizing urine iodine detection by quantitative kit detection urine iodine in the claim 1 is characterized in that concrete steps are:
(1) clears up the serial iodine standard solution that accurately pipettes 50,100,200,300 and 400 μ g/L and each 0.1-1ml of urine sample in test tube, respectively add the 0.2-2ml digestion solution, room temperature leaves standstill after 5 minutes violent jolting test tube repeatedly, and the gas that causes reaction to produce is mainly N
2, O
2With a small amount of Cl
2Till draining, clearing up overall process needs 10-15 minute approximately,
(2) resolve every pipe and add 0.2-2ml and resolve agent, room temperature leaves standstill that violent jolting test tube repeatedly drains the gas that reaction produces after 2 minutes, and overall process must not be less than 10 minutes,
(3) the every pipe of neutralization adds the 0.2-2ml neutralizing agent, and violent jolting test tube repeatedly drains the gas that reaction produces, and overall process must not be less than 10 minutes
(4) sample handled well was placed 10 minutes at the mensuration place, treat temperature balance after every pipe add the 0.2-2ml reductive agent, shake up,
(5) measure every pipe and add the 0.2-2ml indicator, shake up, a selected pipe, add the 0.2-2ml oxygenant fast with sample injector on the other hand, another hand starts manual time-keeping simultaneously, and takes out this test tube immediately and shake up, and this moment, test solution became blueness, carrying out along with reaction, test solution will become bluish violet from blueness, become redness at last, stop stopwatch when test solution becomes bluish violet and just redness occurred, recording reacting time
Repeat above operation, the reaction time of measuring and writing down all the other sample hoses,
(6) calculating of urine content of iodine
Adopt the linear regression equation method to calculate the urine content of iodine, because of logarithm value lnC or the lgC of the logarithm value lnS of reaction time S or lgS and iodine concentration C (μ g/L) linear, obtain the regression equation of typical curve by two recurrence statistical methods, lnC=a+blnS or lgC=a+blgS, a wherein, b is respectively the intercept and the slope of curve, be constant, try to achieve by returning statistical method, it is different constants under different temperatures, regression equation with the reaction time substitution typical curve of sample hose calculates the sample content of iodine.
3, method according to claim 2 is characterized in that iodine standard solution, urine sample after clearing up, and adds to resolve agent or neutralizing agent, adds reductive agent again and reduces.
4, method according to claim 2 is characterized in that neutralizing agent, reductive agent, indicator three or wherein the two mixes the back and adds in advance, neutralizes simultaneously, reduces, indication process or two processes wherein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB01133682XA CN1170147C (en) | 2001-11-16 | 2001-11-16 | Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB01133682XA CN1170147C (en) | 2001-11-16 | 2001-11-16 | Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method |
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| Publication Number | Publication Date |
|---|---|
| CN1348097A CN1348097A (en) | 2002-05-08 |
| CN1170147C true CN1170147C (en) | 2004-10-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB01133682XA Expired - Lifetime CN1170147C (en) | 2001-11-16 | 2001-11-16 | Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101788495B (en) * | 2010-01-29 | 2011-10-26 | 白银有色集团股份有限公司 | Method for measuring arsenic by iodine solution absorption method |
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| CN1320351C (en) * | 2004-09-07 | 2007-06-06 | 上海大学 | Method for measuring casein iodide in fodder |
| CN102495203B (en) * | 2011-11-10 | 2014-01-22 | 武汉众生生化技术有限公司 | Reagent used for detecting urinary iodine by biochemistry analyzer and urinary iodine detecting method |
| CN102636483B (en) * | 2012-05-03 | 2014-04-16 | 李又兰 | Method and device for detecting urine iodine concentration |
| CN105004719A (en) * | 2015-07-21 | 2015-10-28 | 杨先锋 | Urine iodine determination kit and applications thereof |
| CN109540877A (en) * | 2017-09-22 | 2019-03-29 | 王九宏 | Urinate the reagent and detection method that above microplate reader detects after iodine and saliva iodine room temperature pre-treatment |
| CN108776134B (en) * | 2018-08-14 | 2019-11-12 | 厦门市疾病预防控制中心（厦门市卫生检测检验中心） | One kind being based on the iodide ion detection reagent and method of catalysis of iodine hydrazine-[oxidant-Ferroin reagent] system |
| CN109959656B (en) * | 2019-04-10 | 2020-08-07 | 长沙塞克陆德医疗科技有限公司 | Method, equipment and system for automatically detecting concentration of iodine element |
| CN110320207A (en) * | 2019-07-23 | 2019-10-11 | 黄艳丽 | A kind of iodine detection reagent and detection method |
| CN110501966A (en) * | 2019-08-19 | 2019-11-26 | 中国石油天然气集团有限公司 | The control method and device of oil-based drill cuttings thermal desorption process oil content and residence time |
| CN110567948A (en) * | 2019-09-10 | 2019-12-13 | 张鹏 | Iodide ion detection kit |
| CN112945943A (en) * | 2019-12-11 | 2021-06-11 | 文逢乐 | Iodine element detection kit and iodine element detection method |
| CN112082986A (en) * | 2020-09-17 | 2020-12-15 | 湖北文理学院 | Urine iodine detection method |
| CN113358637B (en) * | 2021-05-11 | 2024-02-20 | 棒糖科技(杭州)股份有限公司 | Method for realizing interpretation of semi-quantitative test paper result through stable time |
-
2001
- 2001-11-16 CN CNB01133682XA patent/CN1170147C/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101788495B (en) * | 2010-01-29 | 2011-10-26 | 白银有色集团股份有限公司 | Method for measuring arsenic by iodine solution absorption method |
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| CN1348097A (en) | 2002-05-08 |
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