CN117008311A - Automatic identification system and method for medical cumulus coronal complex - Google Patents
Automatic identification system and method for medical cumulus coronal complex Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000012545 processing Methods 0.000 claims abstract description 74
- 235000013601 eggs Nutrition 0.000 claims abstract description 53
- 210000004027 cell Anatomy 0.000 claims abstract description 32
- 210000001733 follicular fluid Anatomy 0.000 claims abstract description 22
- 210000001519 tissue Anatomy 0.000 claims abstract description 21
- 230000005540 biological transmission Effects 0.000 claims abstract description 17
- 208000007536 Thrombosis Diseases 0.000 claims abstract description 13
- 210000000981 epithelium Anatomy 0.000 claims abstract description 13
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 11
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 11
- 210000004681 ovum Anatomy 0.000 claims abstract description 9
- 238000002834 transmittance Methods 0.000 claims description 27
- 239000013013 elastic material Substances 0.000 claims description 2
- 210000002969 egg yolk Anatomy 0.000 claims 2
- 235000013345 egg yolk Nutrition 0.000 claims 2
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- 238000012360 testing method Methods 0.000 description 8
- 210000001161 mammalian embryo Anatomy 0.000 description 7
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- 210000000287 oocyte Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000001125 extrusion Methods 0.000 description 3
- 230000016087 ovulation Effects 0.000 description 3
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- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
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- 238000000338 in vitro Methods 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
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- 239000007788 liquid Substances 0.000 description 1
- 210000004508 polar body Anatomy 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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Abstract
The invention provides an automatic recognition system and method of a medical egg and crown complex, belonging to the technical field of egg and crown complex recognition, wherein the system comprises a first ocular, a second ocular, a third ocular, a microscope body, an observation display, a processing unit, an objective lens, a microscope light source, a mechanical arm, a constant temperature counting box and a suction component; the inside of microscope body is provided with discernment collection system, and discernment collection system discerns the light information that the microscope light source sent to gather the image information of picking up the ovum ware under the microscope light source shines, processing unit fixes a position different cells or tissues according to the printing opacity condition of light, then counts and marks, then shows through observing the display. The method utilizes the principle of the light transmission condition and the state of follicular fluid, a cumulus oophorus complex, blood clot and epithelial tissue to realize rapid identification of the cumulus oophorus complex, and improves the speed of picking eggs.
Description
Technical Field
The invention belongs to the technical field of recognition of a cumulus compound, and particularly relates to an automatic recognition system and method for a cumulus compound for medical use.
Background
In the diagnosis and treatment work of the reproductive medicine center, a very important link is an egg taking operation of a clinician and an egg picking operation of embryo laboratory staff. In the routine work of embryo laboratories, the operator must be skilled in the "picking up eggs". After female patients conforming to the test tube infant indication undergo ovulation promoting treatment by using ovulation promoting medicines to enable follicles to develop and mature, an egg taking operation is performed, at the moment, a clinician sucks target follicles through vaginal puncture under the guidance of vaginal B ultrasonic, then a follicle-hillock complex in the follicles and follicular fluid are collected in a test tube for sealing (the number of test tubes is variable), the test tube temperature is guaranteed, the test tube temperature is rapidly transmitted to embryo laboratory staff through an egg taking operation room and an embryo laboratory window, and liquid in the test tube is poured into a 3003 dish, and identification and picking of the follicle-hillock complex are performed under a microscope. Wherein the cumulus oophorus complex refers to a complex of oophorus cavities and follicular fluid which appear after growth and differentiation (the diameter can reach 10-20 mm) of follicles to form oocytes, cumulus oophorus and radial granular cells. The clinician and laboratory staff repeat this procedure until the patient's target follicles are collected.
The qualification of the egg picking station is generally that tens of patients need to be picked up continuously, the number of follicles of each patient is required to be minimum, for example, not less than 8, missed picking cannot occur during continuous egg picking, statistics is carried out again once the missed picking occurs, and the egg picking process needs to be fast under the premise of ensuring the temperature so as to reduce the damage of the external environment to the oocytes.
The infertility diagnosis and treatment period is long, so that the physical and psychological fatigue of patients can be caused, and each follicle of each patient after ovulation promotion is extremely precious. After the operation room punctures and picks up the eggs, the clinician rapidly transmits follicular fluid (filled in a test tube) to an embryo laboratory, wherein the follicular fluid possibly contains a follicle-hillock complex, a blood clot and epithelial tissues, and embryo laboratory personnel pour the follicle-hillock complex into a plate under a split microscope to search for the follicle-hillock complex, and transfer the follicle-hillock complex into a G-MOPS plate covered with oil for temporary storage after the follicle-hillock complex is found. In this process, when the extracted cumulus complex contacts the external environment, environmental factors such as temperature change, pH change or light irradiation damage the oocyte, thereby affecting the success of "in vitro insemination-embryo transfer". At the same time, CO 2 Loss is related to surface area, and is faster in a culture dish than in a test tube, so that egg finding and egg picking speeds are faster, in order to quickly find a egg coronal-cumulus complex, after a follicular fluid containing the egg coronal-cumulus complex is poured into the dish, the fluid is spread to a thickness of not more than 0.5cm, embryo laboratory personnel observe under a split microscope, the split microscope is also linked with a display, and an assistant of the egg picking personnel can observe under a display observation mirror. After the follicular fluid is poured into the dish, the follicular fluid is easily recognized by naked eyes in view of the special structure of the cumulus complex, like a mucous mass, but omission sometimes occurs. In order to increase the operation speed of picking up eggs and assist the work of egg picking up staff and ensure that the eggs are not missed to be picked up, a device for assisting in searching and positioning and marking the cumulus complex is designed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides an automatic identification system and an automatic identification method for a medical cumulus coronal complex, and aims to solve the technical problems of improving the egg picking operation speed and avoiding the occurrence of missed picking.
In order to solve the technical problems, the invention provides an automatic identification system of a medical cumulus complex for a egg, which comprises a first ocular, a second ocular, a third ocular, a microscope body, an observation display, a processing unit, an objective lens, a microscope light source, a mechanical arm, a constant temperature counting box and a suction component;
the first ocular, the second ocular and the third ocular are connected above the microscope body, the observation display is electrically connected with the third ocular, the processing unit is electrically connected with the microscope body, the objective lens is connected below the microscope body, the microscope light source is arranged below the objective lens, the mechanical arm is electrically connected with the processing unit, the constant temperature counting box is electrically connected with the processing unit, and one end of the sucking component is connected with the mechanical arm;
the inside of microscope body is provided with discernment collection system, and discernment collection system can discern the light information that the microscope light source sent to gather the image information of ovum dish, then transmit light information and image information to processing unit, processing unit fixes a position, counts and marks target cell or tissue, then shows through observing the display.
The invention provides an automatic identification system of a medical egg and crown complex, which is characterized by comprising a first ocular, a second ocular, a third ocular, a microscope body, a display, a processing unit, an objective lens, a microscope light source, a mechanical arm, a constant temperature counting box and a suction component, wherein the first ocular is a lens;
the first ocular, the second ocular and the third ocular are connected above the microscope body, the display is arranged inside the first ocular or the second ocular, the processing unit is electrically connected with the microscope body, the objective lens is connected below the microscope body, the microscope light source is arranged below the objective lens, the mechanical arm is electrically connected with the processing unit, the constant temperature counting box is electrically connected with the processing unit, and one end of the sucking component is connected with the mechanical arm;
the inside of microscope body is provided with discernment collection system, and discernment collection system can discern the light information that the microscope light source sent to gather the image information of ovum dish, then transmit light information and image information to processing unit, processing unit is to target cell or tissue location, count and mark, then show through the display.
Further, a plurality of compartments are arranged in the constant temperature counting box.
Further, a temperature control device is arranged in the constant temperature counting box.
Further, the constant temperature counting box is characterized in that a counting device is arranged inside the constant temperature counting box.
Further, the inside of the lateral wall of constant temperature count box is provided with flexible shading lid.
Further, the suction member includes a suction portion and a pressing portion, and the pressing portion is connected to an upper end of the suction portion.
Further, the pressing portion is made of an elastic material.
Further, the suction part is a glass Pasteur pipette.
The invention provides an automatic identification method of a medical cumulus and a cumulus compound, which comprises the following steps of:
step S1: placing the egg picking dish between the objective lens and the microscope light source;
step S2: the recognition acquisition device recognizes the light information transmitted through the egg picking dish and acquires the image information of the egg picking dish irradiated by the microscope light source;
step S3: the identification acquisition device transmits the light information and the position information to the processing unit, and the processing unit positions, counts and marks the cumulus complex and then displays the cumulus complex through the display or the observation display;
step S4: the processing unit commands the mechanical arm to control the suction component to pick up the cumulus oophorus complex according to the marks in the display, so as to finish egg picking.
In the step S2, the identification and collection device identifies the light information transmitted through the egg-picking dish, and according to the light transmission condition of the light passing through follicular fluid, the cumulus of the egg-crowns, blood clots and epithelial tissues, the three levels of high transmittance, medium transmittance and poor transmittance are divided, the light transmittance level of follicular fluid is high, the light transmittance level of blood clot and epithelial tissue is poor, and the light transmittance level of the cumulus complex is medium transmittance.
Further, in step S3, the processing unit identifies and locates the cumulus complex in the image information transmitted by the identification and acquisition device according to the light transmission condition of the cumulus complex, and then marks and counts the cumulus complex.
Further, in the step S4, the staff member sets the constant temperature counting box to a set temperature.
Further, in the step S4, the constant temperature counting box counts the placed cumulus complexes, after the picking is finished, the constant temperature counting box inputs the calculated quantity data into the processing unit, compares the calculated quantity data with the number of the cumulus complexes counted by the processing unit in the step S3, and if the calculated quantity data is inconsistent, the processing unit alarms through a display or an observation display.
Further, in the step S4, after the eggs are picked up in a row or a column of cells in the constant temperature counting box, the telescopic light shielding cover of the constant temperature counting box extends out under the control of the processing unit to cover the cells or tissues in the row.
According to the automatic recognition system of the medical cumulus oophorus complex, the light transmission condition and the state of the follicular fluid, the cumulus oophorus complex, blood clots and epithelial tissues are utilized, a recognition acquisition device is arranged in a microscope body to recognize light information sent by a microscope light source which is upwards transmitted through a cumulus oophorus picking plate, the cumulus oophorus complex is recognized according to the light brightness, and a processing unit is utilized to position and mark the cumulus oophorus complex, so that the rapid recognition of the cumulus oophorus complex is realized; counting the cumulus complexes by using a processing unit, and comparing the cumulus complexes with the count of a constant temperature counting box to avoid missed picking; the processing unit is used for controlling the mechanical arm and the suction component to pick up the cumulus coronal complex, so that the automatic picking up of the cumulus coronal complex is realized, and the speed of picking up eggs is improved; the telescopic shading cover is arranged in the constant temperature counting box, so that the influence of light on the cumulus complex can be avoided; the temperature control device is arranged in the constant temperature counting box and can control according to the temperature required by the cumulus compound, so that the influence of the temperature on the cumulus compound is avoided.
The automatic recognition method of the medical cumulus oophorus complex provided by the invention utilizes the principle that the different light transmission conditions and the state of follicular fluid, the cumulus oophorus complex, blood clot and epithelial tissue are present, so that the cumulus oophorus complex is rapidly recognized, and the speed of picking eggs is improved.
Drawings
Fig. 1 is a schematic structural view of an automatic identification system for a medical cumulus complex.
Wherein 1-a first eyepiece; 2-a second eyepiece; 3-a third eyepiece; 4-a microscope body; 5-viewing a display; a 6-processing unit; 7-an objective lens; 8-a microscope light source; 9-a mechanical arm; 10-a constant temperature counting box; 11-suction means.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In order to better understand the purpose, structure and function of the present invention, the following describes in further detail a medical automatic identification system for a cumulus complex and a method thereof with reference to the accompanying drawings.
Example 1:
as shown in fig. 1, the invention provides an automatic recognition system of a medical cumulus complex, which comprises a first ocular lens 1, a second ocular lens 2, a third ocular lens 3, a microscope body 4, an observation display 5, a processing unit 6, an objective lens 7, a microscope light source 8, a mechanical arm 9, a constant temperature counting box 10 and a suction component 11;
the first ocular 1, the second ocular 2 and the third ocular 3 are connected above the microscope body 4, the observation display 5 is electrically connected with the third ocular 3, the processing unit 6 is electrically connected with the microscope body 4, the objective lens 7 is connected below the microscope body 4, the microscope light source 8 is arranged below the objective lens 7, the mechanical arm 9 is electrically connected with the processing unit 6, the constant temperature counting box 10 is electrically connected with the processing unit 6, and one end of the sucking component 11 is connected with the mechanical arm 9;
the inside of microscope body 4 is provided with discernment collection system, and discernment collection system can discern the light information that the microscope light source sent to gather the image information of ovum dish, then transmit light information and image information to processing unit 6, processing unit 6 is to target cell or tissue location, count and mark, then shows through observing display 5.
Example 2:
the invention provides an automatic identification system of a medical egg and crown complex, which comprises a first ocular lens 1, a second ocular lens 2, a third ocular lens 3, a microscope body 4, a display, a processing unit 6, an objective lens 7, a microscope light source 8, a mechanical arm 9, a constant temperature counting box 10 and a suction component 11, wherein the first ocular lens is a lens;
the first ocular lens 1, the second ocular lens 2 and the third ocular lens 3 are connected above the microscope body 4, the display is arranged inside the first ocular lens 1 or the second ocular lens 2, the processing unit 6 is electrically connected with the microscope body 4, the objective lens 7 is connected below the microscope body 4, the microscope light source 8 is arranged below the objective lens 7, the mechanical arm 9 is electrically connected with the processing unit 6, the constant temperature counting box 10 is electrically connected with the processing unit 6, and one end of the sucking component 11 is connected with the mechanical arm 9;
the inside of microscope body 4 is provided with discernment collection system, and discernment collection system can discern the light information that the microscope light source sent to gather the image information of ovum dish, then with light information and image information transmission to processing unit 6, processing unit 6 is to target cell or tissue location, count and mark, then show through the display.
A plurality of compartments are arranged in the constant temperature counting box 10, and each of the cumulus complexes is placed in one compartment; the temperature control device is arranged in the constant temperature counting box 10, so that a worker can adjust the temperature required by cells or tissues according to the needs, and the damage of the indoor temperature to the cells or tissues is reduced;
the constant temperature counting box 10 is internally provided with a counting device for counting cells or tissues put into the constant temperature counting box 10, and the cells or tissues are compared with the count in the processing unit 6 after the counting is finished, so that missed picking is prevented;
the top of constant temperature count box 10 is provided with flexible shading lid, and flexible shading lid sets up inside the lateral wall of constant temperature count box 10, after accomplishing the ovum of picking up of a row of check, stretches out under the control of processing unit 6, covers this cell or tissue of a row, avoids light to cause the harm to the cell.
The suction member 11 includes a suction portion and an extrusion portion, the extrusion portion is made of a silicone head or a rubber head, the mechanical arm 9 extrudes the extrusion portion to form negative pressure under the control of the processing unit 6, and the suction portion is a glass tube to suck the target cell tissue.
The working process of the automatic identification system for the medical coronal and cumulus complex is as follows:
the egg collection dish is placed between the objective lens 7 and the microscope light source 8, and the collected follicular fluid may contain a cumulus complex, a blood clot, and epithelial tissue. The follicular fluid is best in general colorless and transparent (blood is sometimes mixed to be red due to the technical problem of egg taking operation, and certain light transmittance is also caused at the moment), blood clots are red and opaque, epithelial tissues are white/yellow and opaque, the follicles and the follicles are in a shape of 'round dot opaque and surrounding transparent' (when the follicular fluid is blood stained to be red, the periphery of the follicles and the follicles are easier to find, because the periphery of the follicles and the follicles are not stained with blood), the opaque part of the round dot in the middle of the follicles and the follicles are actually oocytes, a polar body with a relatively small volume is wrapped with an extracellular matrix transparent belt mainly formed by oocyte secretion, and the surrounding transparent part is actually transparent granular cells).
According to the light transmission condition that light passes through each cell or tissue or follicular fluid, divide into transmittance height, transmittance medium and the poor three level of transmittance, the light transmission level of follicular fluid is transmittance height, and the light transmission level of blood clot and epithelial tissue is the transmittance poor, and the light transmission level of ovum crown-cumulus complex is transmittance medium etc..
The recognition acquisition device recognizes the light information transmitted through the egg picking dish, can divide the light transmission levels of different cells or tissues or follicular fluid according to the light transmission condition of the light, and acquires the image information of the egg picking dish irradiated by the microscope light source 8; then transmitting the light information and the image information to a processing unit 6, positioning the target cells (the cumulus complexes) in the image information transmitted by the identification acquisition device according to the light transmission condition of the target cells (the cumulus complexes), marking and counting, marking by different colors, and displaying by a display;
the processing unit 6 commands the mechanical arm 9 to control the suction component 11 to pick up target cells or tissues according to the marks in the display;
the suction component 11 is used for placing the picked-up cumulus complexes in the constant temperature counting box 10 for heat preservation, after the picking-up is finished, the constant temperature counting box 10 can input the number of the cumulus complexes in the processing unit 6, the number is compared with the number of cells or tissues counted by the processing unit 6 before, and if the number is inconsistent, the processing unit 6 alarms through a display.
Example 3:
the invention provides an automatic identification method of a medical cumulus complex, which comprises the following steps:
step S1: placing an egg picking dish between the objective lens 7 and the microscope light source 8;
step S2: the recognition acquisition device recognizes the light information transmitted through the egg picking up dish and acquires the image information of the egg picking up dish irradiated by the microscope light source 8;
step S3: the recognition acquisition device transmits the light information and the image information to the processing unit 6, and the processing unit 6 positions, counts and marks the cumulus complexes and then displays the cumulus complexes through the display;
step S4: the processing unit 6 commands the mechanical arm 9 to control the suction component 11 to pick up target cells or tissues according to the marks in the display, and the constant temperature counting box 10 counts the placed cumulus complexes to finish picking up eggs.
In the step S2, the identification and collection device identifies the light information transmitted through the egg-picking dish, and according to the light transmission condition of the light passing through follicular fluid, a cumulus of a follicle, blood clots and epithelial tissues, the three levels of high transmittance, medium transmittance and poor transmittance are divided, the light transmittance level of follicular fluid is high, the light transmittance level of blood clot and epithelial tissue is poor, and the light transmittance level of the cumulus complex is medium transmittance.
In step S3, the processing unit 6 locates the target cell cumulus complex in the image information transmitted by the identification and acquisition device according to the light transmission condition of the cumulus complex, and then marks and counts the target cell cumulus complex.
In the step S4, after the constant temperature counting box 10 is set to a set temperature, the suction component 11 places the picked-up cumulus complexes in the constant temperature counting box 10 for heat preservation, after the picking is finished, the constant temperature counting box 10 inputs the number data of the cumulus complexes in the interior into the processing unit 6, and compares the number data with the number of the cumulus complexes counted by the processing unit 6 in the step S3, if the number data is inconsistent, the processing unit 6 alarms through a display, thereby avoiding the occurrence of missed picking.
In the step S4, after the egg picking in a row or a column of cells is completed, the telescopic light shielding cover extends out under the control of the processing unit 6 to cover the cells or tissues in the row, so as to avoid damage to the cells caused by light.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.
Claims (15)
1. The automatic identification system of the medical cumulus complex for the egg crowns is characterized by comprising a first ocular, a second ocular, a third ocular, a microscope body, an observation display, a processing unit, an objective lens, a microscope light source, a mechanical arm, a constant temperature counting box and a suction component;
the first ocular, the second ocular and the third ocular are connected above the microscope body, the observation display is electrically connected with the third ocular, the processing unit is electrically connected with the microscope body, the objective lens is connected below the microscope body, the microscope light source is arranged below the objective lens, the mechanical arm is electrically connected with the processing unit, the constant temperature counting box is electrically connected with the processing unit, and one end of the sucking component is connected with the mechanical arm;
the inside of microscope body is provided with discernment collection system, and discernment collection system can discern the light information that the microscope light source sent to gather the image information of ovum dish, then transmit light information and image information to processing unit, processing unit fixes a position, counts and marks target cell or tissue, then shows through observing the display.
2. The automatic identification system of the medical cumulus complex for the egg crowns is characterized by comprising a first ocular, a second ocular, a third ocular, a microscope body, a display, a processing unit, an objective lens, a microscope light source, a mechanical arm, a constant temperature counting box and a suction component;
the first ocular, the second ocular and the third ocular are connected above the microscope body, the display is arranged inside the first ocular or the second ocular, the processing unit is electrically connected with the microscope body, the objective lens is connected below the microscope body, the microscope light source is arranged below the objective lens, the mechanical arm is electrically connected with the processing unit, the constant temperature counting box is electrically connected with the processing unit, and one end of the sucking component is connected with the mechanical arm;
the inside of microscope body is provided with discernment collection system, and discernment collection system can discern the light information that the microscope light source sent to gather the image information of ovum dish, then transmit light information and image information to processing unit, processing unit is to target cell or tissue location, count and mark, then show through the display.
3. The automatic identification system of a medical cumulus complex for a egg yolk according to any one of claims 1-2, wherein a plurality of compartments are provided inside the constant temperature counting box.
4. The automatic identification system for a medical cumulus complex for a egg according to claim 3, wherein a temperature control device is arranged in the constant temperature counting box.
5. The automatic identification system for a medical cumulus complex for a egg according to claim 3, wherein a counting device is arranged in the constant temperature counting box.
6. The automatic identification system for a medical cumulus complex for a egg yolk according to claim 3, wherein a telescopic light shielding cover is arranged inside a side wall of the constant temperature counting box.
7. The automatic identification system of a medical cumulus complex for a egg according to any one of claims 1-2, wherein the suction means comprises a suction portion and a pressing portion, and the pressing portion is connected to an upper end of the suction portion.
8. The automatic identification system of a medical cumulus complex for a egg according to claim 7, wherein the pressing portion is made of an elastic material.
9. The automatic identification system of a medical cumulus complex for a egg according to claim 7, wherein the suction part is a pasteur pipette.
10. An automatic identification method of a medical cumulus coronal complex, characterized in that the automatic identification system of the medical cumulus coronal complex according to any one of claims 1-2 is used, comprising the following steps:
step S1: placing the egg picking dish between the objective lens and the microscope light source;
step S2: the recognition acquisition device recognizes the light information transmitted through the egg picking dish and acquires the image information of the egg picking dish irradiated by the microscope light source;
step S3: the identification acquisition device transmits the light information and the position information to the processing unit, and the processing unit positions, counts and marks the cumulus complex and then displays the cumulus complex through the display or the observation display;
step S4: the processing unit commands the mechanical arm to control the suction component to pick up the cumulus oophorus complex according to the marks in the display, so as to finish egg picking.
11. The automatic recognition method of a medical cumulus complex for a egg, as set forth in claim 10, wherein in the step S2, the recognition and acquisition device recognizes the light information transmitted through the egg-picking dish, and the light transmission of the light through the follicular fluid, the cumulus complex, the blood clot and the epithelial tissue is classified into three levels of high transmittance, medium transmittance and poor transmittance, the follicular fluid is high in transmittance, the blood clot and the epithelial tissue are poor in transmittance, and the cumulus complex is medium in transmittance.
12. The method according to claim 11, wherein in the step S3, the processing unit identifies and locates the cumulus complex in the image information transmitted by the identification and acquisition device according to the light transmission condition of the cumulus complex, and then marks and counts the cumulus complex.
13. The method for automatically identifying a medical cumulus complex according to claim 10, wherein in the step S4, the worker sets the constant temperature counting box to a set temperature.
14. The automatic identification method of a medical cumulus complex according to claim 10, wherein in the step S4, the thermostatic counting box counts the number of the placed cumulus complex, after the picking is finished, the thermostatic counting box inputs the calculated number data into the processing unit, and compares the calculated number with the number of the cumulus complex counted by the processing unit in the step S3, and if the number is inconsistent, the processing unit alarms through a display or an observation display.
15. The method according to claim 10, wherein in the step S4, after the eggs are picked up in one row or one column of cells in the constant temperature counting box, the retractable light-shielding cover of the constant temperature counting box is extended under the control of the processing unit to cover the cells or tissues in the one row.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117982214A (en) * | 2024-04-07 | 2024-05-07 | 安徽医科大学第一附属医院 | Auxiliary identification method and system for rapid egg picking |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117982214A (en) * | 2024-04-07 | 2024-05-07 | 安徽医科大学第一附属医院 | Auxiliary identification method and system for rapid egg picking |
| CN117982214B (en) * | 2024-04-07 | 2024-07-23 | 安徽医科大学第一附属医院 | Auxiliary identification method and system for rapid egg picking |
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