CN116947971A - Antibacterial peptide and application thereof - Google Patents
Antibacterial peptide and application thereof Download PDFInfo
- Publication number
- CN116947971A CN116947971A CN202310725014.9A CN202310725014A CN116947971A CN 116947971 A CN116947971 A CN 116947971A CN 202310725014 A CN202310725014 A CN 202310725014A CN 116947971 A CN116947971 A CN 116947971A
- Authority
- CN
- China
- Prior art keywords
- antibacterial peptide
- application
- pseudomonas fluorescens
- seq
- micrococcus luteus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003910 polypeptide antibiotic agent Substances 0.000 title claims abstract description 16
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 4
- 241000191938 Micrococcus luteus Species 0.000 claims description 14
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 14
- 241000194056 Streptococcus iniae Species 0.000 claims description 12
- 102000044503 Antimicrobial Peptides Human genes 0.000 claims description 5
- 108700042778 Antimicrobial Peptides Proteins 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 5
- 208000035143 Bacterial infection Diseases 0.000 claims description 3
- 208000022362 bacterial infectious disease Diseases 0.000 claims description 2
- 239000003112 inhibitor Substances 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 8
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 7
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- 241000192125 Firmicutes Species 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000031729 Bacteremia Diseases 0.000 description 1
- 206010012455 Dermatitis exfoliative Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to the field of molecular biology, in particular to two antibacterial peptides and application thereof. The antibacterial peptide is shown in the amino acid sequences in the sequence tables SEQ ID No.1 and SEQ ID No. 2. The application of the antibacterial peptide in preparing a bactericidal preparation.
Description
Technical Field
The invention relates to the field of molecular biology, in particular to two antibacterial peptides and application thereof.
Background
An antimicrobial peptide (antimicrobial peptide) is a polypeptide with antimicrobial activity, generally having a strong alkalinity, and playing an important role in innate immunity. Antibacterial peptides are found in almost all living species and exert biological activities against bacteria, viruses, fungi, parasites, etc. Compared with antibiotics, the antibacterial peptide has the characteristics of environmental protection and no drug resistance of target bacteria, thus having wide application prospect. Pseudomonas fluorescens (Pseudomonas fluorescens) belongs to gram-negative aerobic bacillus, is a conditional pathogenic bacterium for aquatic animals, and can infect various seawater and freshwater animals to cause septicemia and erythroderma. Streptococcus ragmitis (Streptococcus iniae) is gram-positive bacteria, can cause streptococcicosis of fish, and is an important pathogenic bacteria of various marine fishes and freshwater fishes. Micrococcus luteus (Micrococcus luteus) belongs to gram-positive bacteria, is widely distributed in living environments such as soil, air, water and the like and animal body surfaces, can infect human beings, and causes bacteremia and the like. At present, few antibacterial peptides which have killing effects on Pseudomonas fluorescens, streptococcus iniae and Micrococcus luteus are reported.
Disclosure of Invention
The invention aims to provide two antibacterial peptides and application thereof.
In order to achieve the above purpose, the invention adopts the following technical scheme:
an antibacterial peptide is shown in the amino acid sequence of SEQ ID No.1 or SEQ ID No.2 of a sequence table.
Use of said antimicrobial peptide for the preparation of an inhibitor against bacterial infection.
The bacteria are Pseudomonas fluorescens (Pseudomonas fluorescens), streptococcus iniae (Streptococcus iniae) or Micrococcus luteus (Micrococcus luteus).
Drawings
FIG. 1 shows the killing effect of the antibacterial peptide provided by the embodiment of the invention on bacteria.
Detailed Description
The invention is further illustrated below with reference to examples. The examples are intended to illustrate the invention and not to limit it in any way.
Example 1
The artificially designed antibacterial peptides (named BcP and DrP) are respectively the amino acid sequences of SEQ ID No.1 and SEQ ID No.2 in the sequence table.
The sequence table SEQ ID No.1 is:
KCFLRRRRSRA
(a) Sequence characteristics:
● Length: 11
● Type (2): amino acid sequence
● Chain type: single strand
● Topology structure: linearity of
(b) Molecular type: proteins
(c) Assume that: whether or not
(d) Antisense: whether or not
(e) The initial source is: artificial synthesis
The sequence table SEQ ID No.2 is:
MKRRRKFSHFSFKRGY
(a) Sequence characteristics:
● Length: 16
● Type (2): amino acid sequence
● Chain type: single strand
● Topology structure: linearity of
(b) Molecular type: proteins
(c) Assume that: whether or not
(d) Antisense: whether or not
(e) The initial source is: artificial synthesis
Example 2
Application of antibacterial peptide
Step 1) bacterial preparation.
Culturing Pseudomonas fluorescens, streptococcus iniae and Micrococcus luteus in LB medium to OD 600 0.8 and then centrifuged (5000 g,4 ℃ C.) for 10min. The cells were collected and suspended in PBS to a final concentration of 5X10 3 cfu/ml。
The LB comprises the following components in percentage by weight: 1.0% peptone, 0.5% yeast powder, 1.0% sodium chloride, 97.5% distilled water. The PBS comprises the following components in percentage by weight: 0.8% NaCl,0.02% KCl,0.358% Na 2 HPO 4 .12H 2 O,0.024％ NaH 2 PO 4 。
The Pseudomonas fluorescens (Pseudomonas fluorescens) is stored in China general microbiological culture collection center (CGMCC) of China general microbiological culture Collection center, and the storage number is: CGMCC No.2329. The streptococcus iniae (Streptococcus iniae) is stored in China general microbiological culture collection center (CGMCC) with the storage number of: CGMCC No.1984. The micrococcus luteus (Micrococcus luteus) is stored in CGMCC with the number of CGMCC 1.191.
Step 2) antibacterial effect of BcP and DrP 16.
100ul of the Pseudomonas fluorescens liquid, streptococcus iniae liquid and Micrococcus luteus liquid of the step 1) above were mixed with the antibacterial peptide BcP (20. Mu.M final concentration), drP16 (20. Mu.M final concentration) or PBS (control), respectively, and incubated at 22℃for 4 hours. The mixture was then spread on LB solid plates, and after culturing for 32-48 hours, the growth conditions of Pseudomonas fluorescens (left in FIG. 1A), streptococcus ragmitis (left in FIG. 1B) and Micrococcus luteus (left in FIG. 1C) were observed. At the same time, colony counts were performed. The results showed that the pseudomonas fluorescens (right in fig. 1A), streptococcus iniae (right in fig. 1B) and micrococcus luteus (right in fig. 1C) in the BcP and DrP16 treated groups survived almost no more than the PBS treated groups, i.e., the sterilization rates of BcP11 and DrP16 were about 100%.
These results indicate that BcP and DrP have high antimicrobial effect and can be applied to the prevention and treatment of bacterial diseases related to Pseudomonas fluorescens, streptococcus iniae and Micrococcus luteus.
Claims (3)
1. An antimicrobial peptide, characterized in that: the antibacterial peptide is shown in the amino acid sequence of SEQ ID No.1 or SEQ ID No.2 of the sequence table.
2. Use of the antimicrobial peptide of claim 1, wherein: the antibacterial peptide is used for preparing an inhibitor for resisting bacterial infection.
3. Use according to claim 2, characterized in that: the bacteria are Pseudomonas fluorescens (Pseudomonas fluorescens), streptococcus iniae (Streptococcus iniae) or Micrococcus luteus (Micrococcus luteus).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310725014.9A CN116947971A (en) | 2023-06-19 | 2023-06-19 | Antibacterial peptide and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310725014.9A CN116947971A (en) | 2023-06-19 | 2023-06-19 | Antibacterial peptide and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116947971A true CN116947971A (en) | 2023-10-27 |
Family
ID=88459471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310725014.9A Pending CN116947971A (en) | 2023-06-19 | 2023-06-19 | Antibacterial peptide and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116947971A (en) |
-
2023
- 2023-06-19 CN CN202310725014.9A patent/CN116947971A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU618714B2 (en) | Novel bacteriocin compositions for use as enhanced broad range bactericides and methods of preventing and treating microbial infection | |
| CN112940082B (en) | Antibacterial peptide and application thereof | |
| US7026353B2 (en) | Inhibition of Gram positive bacteria | |
| KR101655781B1 (en) | Bacillus tequilensis HD15 with antibacterial activity and purification method of bacteriocin using the same | |
| CN102167736A (en) | Green mud crab antibacterial peptide Sphistin and application thereof | |
| US20210317172A1 (en) | Antimicrobial peptide scyreprocin of scylla paramamosain and method thereof | |
| Dewasthale et al. | Microbial biofilm: current challenges in health care industry | |
| Lim et al. | Synergistic antimicrobial effect of a seaweed-probiotic blend against acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus | |
| CN116947971A (en) | Antibacterial peptide and application thereof | |
| Aunpad et al. | Isolation and characterization of bacteriocin with anti-listeria and anti-MRSA activity produced by food and soil isolated bacteria | |
| KR100407074B1 (en) | Antimicrobial Peptide-Producing Microorganism from Korean Fermented Fishes, Jeotkal in Korean | |
| CN115536737A (en) | Application of cobra antibacterial peptide OH-CATH30 in resisting aquatic animal pathogenic bacteria | |
| CN116284248A (en) | Antibacterial peptide and antibacterial application thereof | |
| KR101970656B1 (en) | Novel Streptomyces lienomycini DS620 Strain and Antimicrobial Composition Comprising Extract Thereof | |
| CN112625935A (en) | MRS solid culture medium, microbial compound bacterium agent for inhibiting staphylococcus aureus and preparation method thereof | |
| Ranjini et al. | Bactericidal activity of skin mucus and skin extracts of Catla catla and Channa striatus | |
| KR20220037035A (en) | Antibacterial peptide derived from Haliotis madaka | |
| Masitoh et al. | Antimicrobial activity of Bacillus cereus and Bacillus thuringiensis on pathogenic Vibrio harveyi in Litopenaeus vannamei | |
| CN110974838A (en) | Application of gold compound in preparation of antibacterial agent | |
| Temario et al. | Inhibitory activity of probiotic Bacillus subtilis BF12 against Vibrio parahaemolyticus infection and its growth-promoting effects on juvenile Penaeus monodon | |
| Popova et al. | Investigation on the microflora of the longtailed mealybug Pseudococcus longispinus (Targioni-Tozzetti)(Hemiptera: Pseudococcidae) in order to assess its importance as a carrier of pathogenic microorganisms | |
| CN114097786B (en) | Method for completely killing vibrio splendidus | |
| CN106957353B (en) | Novel polypeptide capable of achieving broad-spectrum antibacterial effect and application thereof | |
| KR101461096B1 (en) | Biofilm formation inhibitors comprising streptomyces sp. or kribbella sp. and a method using thereof | |
| CN113528461B (en) | Isolated aeromonas salmonicida phage, compositions and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |