CN116924454A - Preparation method of silver oxide nanospheres and application of silver oxide nanospheres in organophosphorus pesticide detection - Google Patents

Preparation method of silver oxide nanospheres and application of silver oxide nanospheres in organophosphorus pesticide detection Download PDF

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CN116924454A
CN116924454A CN202211371649.5A CN202211371649A CN116924454A CN 116924454 A CN116924454 A CN 116924454A CN 202211371649 A CN202211371649 A CN 202211371649A CN 116924454 A CN116924454 A CN 116924454A
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silver oxide
solution
detection
organophosphorus
oxide nanospheres
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景文杰
马红艳
强珊
贾泽君
孟璇
戴玉杰
张黎明
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Jingyuan Industrial Technology Research Institute
Tianjin University of Science and Technology
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Tianjin University of Science and Technology
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract

The application provides a preparation method of silver oxide nanospheres and application thereof in organophosphorus pesticide detection, wherein the preparation method comprises the following steps: dropwise adding ammonia water into the silver nitrate solution in the stirring process to form a mixed solution; dropwise adding sodium hydroxide solution into the mixed solution to obtain a precipitate; collecting the precipitate, and standing for 12 hours at room temperature in a dark place; washing and light-shielding drying the precipitate after standing to obtain the silver oxide nanospheres. The silver oxide nanospheres prepared by the application do not need to add natural acetylcholinesterase in the process of detecting organophosphorus pesticides, well overcome the defects of unstable natural enzymes, easy inactivation, high price and the like, and simultaneously can distinguish various organophosphorus pesticides simultaneously through cross response by catalyzing various chromogenic substrates.

Description

Preparation method of silver oxide nanospheres and application of silver oxide nanospheres in organophosphorus pesticide detection
Technical Field
The application relates to the technical field of biosensing analysis, in particular to a preparation method of silver oxide nanospheres and application of the silver oxide nanospheres in organophosphorus pesticide detection.
Background
Pesticides are often widely used to control or eliminate pests and weeds to increase and improve crop yields and quality. The organic phosphorus pesticide has relatively high persistence under natural conditions, and has higher effectiveness in killing diseases and insects compared with other types of pesticides, so that the organic phosphorus pesticide is widely applied to the production process of various crops. However, excessive or unreasonable use of organophosphorus pesticides causes pesticide residues in environmental media such as crops, soil and water, which pose a great threat to human health and the environment. Nowadays, although various detection methods including chromatography, enzyme-linked immunosorbent, electrochemical analysis, surface-enhanced raman scattering technique, etc. have been developed to detect various pesticides, these detection methods have high sensitivity and specificity. Unfortunately, however, these detection methods have problems, such as chromatographic methods typically require relatively expensive instrumentation and cumbersome pretreatment of the sample, enzyme-linked immunosorbent often rely on specific antibodies for participation, and electrochemical methods are susceptible to environmental interference.
It is appreciated that in recent years, biosensors constructed based on nanoenzymes have been studied and applied to organophosphorus pesticide detection. The detection principle is that nano enzyme with peroxidase-like activity is combined with natural enzyme to construct multi-step cascade catalytic reaction, according to the characteristic that the organophosphorus pesticide can inhibit the activity of acetylcholinesterase (AChE) (the phosphate group in the organophosphorus pesticide can react with the active group of AChE,thereby destroying the active site of AChE, resulting in inactivation of AChE) thereby affecting the production of H 2 O 2 The amount of the organic phosphorus pesticide further influences the amount of generated oxTMB, and the colorimetric detection of the organic phosphorus pesticide is realized.
Although sensors constructed based on nanoenzymes have achieved a relatively efficient detection of organophosphorus pesticides and exhibit good sensitivity and specificity. However, there are still some problems: (1) At present, the detection of the pesticide is designed to be carried out by the participation of natural enzymes, and the natural enzymes are easy to inactivate, so false positive signals often appear in the actual detection, and the actual detection is not feasible. (2) Most reports at present only detect one to two organophosphorus pesticides (only one of the most reports), and cannot detect and distinguish multiple organophosphorus pesticides simultaneously. In particular, for a wide variety of organophosphorus pesticides, under one condition, the number of detectable tests by one detection means is limited, and for detection of a plurality of organophosphorus pesticides, a plurality of methods or multiple determinations under a plurality of conditions are often required, so that the actual detection is limited. Therefore, it is needed to solve the problem of simultaneous detection and discrimination of multiple organophosphorus pesticides by combining the advantages of nanoenzymes in the field of organophosphorus pesticide detection with other means.
Disclosure of Invention
In order to overcome the defects of the prior art, the application aims to provide a preparation method of silver oxide nanospheres and application of the silver oxide nanospheres in organophosphorus pesticide detection.
In order to achieve the above object, the present application provides the following solutions:
a method for preparing silver oxide nanospheres, comprising:
dropwise adding ammonia water into the silver nitrate solution in the stirring process to form a mixed solution;
dropwise adding sodium hydroxide solution into the mixed solution to obtain a precipitate;
collecting the precipitate, and standing for 12 hours at room temperature in a dark place;
washing and light-shielding drying the precipitate after standing to obtain the silver oxide nanospheres.
Preferably, the concentration of the ammonia water is 0.05M, the concentration of the silver nitrate solution is 0.05M, and the concentration of the sodium hydroxide solution is 2M.
Preferably, the volume ratio of the ammonia water, the silver nitrate solution and the sodium hydroxide solution is 20:10:1.
preferably, the precipitate is dried in the dark for 12 hours at a temperature of 45 ℃.
The application also provides a method for applying the silver oxide nanospheres in organophosphorus pesticide detection, which comprises the following steps:
adding silver oxide nanosphere solution into organophosphorus pesticide to be detected to form pesticide detection solution;
adding a chromogenic substrate into the pesticide detection liquid, and shaking uniformly to obtain a solution to be analyzed;
and carrying out colorimetric detection on the solution to be analyzed to obtain the class or concentration of the corresponding organophosphorus pesticide.
Preferably, the organophosphorus pesticide comprises any one or more of phoxim, chlorpyrifos, dimethoate, triazophos, methylparathion and trichlorfon.
Preferably, the chromogenic substrate comprises: 3,3', 5' -tetramethylbenzidine, o-phenylenediamine and 2,2' -azido-bis (3-ethylbenzothiazoline-6-sulfonic acid).
Preferably, in the solution to be analyzed, the concentration of the silver oxide nanosphere solution is 15-50 mug/mL, the concentration of 3,3', 5' -tetramethylbenzidine, o-phenylenediamine and 2,2' -azido-bis (3-ethylbenzothiazoline-6-sulfonic acid) is 2mM, 5mM and 2.5mM respectively, and the concentration of the organophosphorus pesticide is 1-100ng/mL.
Preferably, colorimetric detection is performed on the solution to be analyzed to obtain the category or concentration of the corresponding organophosphorus pesticide, which comprises the following steps:
placing the solution to be analyzed on a 96-well plate, and shooting the 96-well plate by using a mobile phone to obtain a shooting picture;
the APP in the mobile phone is utilized to carry out color taking on the photo to obtain RGB values of the corresponding organophosphorus pesticides;
and (5) completing the differentiation of the corresponding organophosphorus pesticide types according to the RGB values.
According to the specific embodiment provided by the application, the application discloses the following technical effects:
the application provides a preparation method of silver oxide nanospheres and application thereof in organophosphorus pesticide detection, wherein the preparation method comprises the following steps: dropwise adding ammonia water into the silver nitrate solution in the stirring process to form a mixed solution; dropwise adding sodium hydroxide solution into the mixed solution to obtain a precipitate; collecting the precipitate, and standing for 12 hours at room temperature in a dark place; washing and light-shielding drying the precipitate after standing to obtain the silver oxide nanospheres. The silver oxide nanospheres prepared by the application do not need to add natural acetylcholinesterase in the process of detecting organophosphorus pesticides, well overcome the defects of unstable natural enzymes, easy inactivation, high price and the like, and simultaneously can distinguish various organophosphorus pesticides simultaneously through cross response by catalyzing various chromogenic substrates.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 shows the Ag prepared according to this application in example 1 2 Scanning electron microscope images of O nanospheres;
FIG. 2 shows the Ag prepared according to this application in example 1 2 X-ray diffraction pattern of O nanospheres;
FIG. 3 is a schematic diagram of example 2 based on Ag 2 The three-channel array sensor constructed by the O nanospheres is used for identifying a schematic diagram of various organophosphorus pesticides;
FIG. 4 is a schematic diagram of example 2 based on Ag 2 The three-channel array sensor constructed by the O nanospheres realizes a distinguishing detection diagram of six organophosphorus pesticides under the condition of 10ng/mL concentration;
FIG. 5 is a diagram based on Ag in example 3 2 The three-channel colorimetric array sensor constructed by the O nanospheres realizes a distinguishing detection diagram of the dimethoate with different concentrations;
FIG. 6 is a schematic diagram of example 4Based on Ag 2 The three-channel colorimetric array sensor constructed by the O nanospheres realizes a distinguishing detection diagram of binary mixed organophosphorus pesticides phoxim and trichlorfon;
FIG. 7 is a diagram based on Ag in example 5 2 The three-channel colorimetric array sensor constructed by the O nanospheres realizes a distinguishing detection diagram of organophosphorus pesticides in melon epidermis cleaning liquid;
FIG. 8 is a diagram based on Ag in example 6 2 The three-channel colorimetric array sensor constructed by the O nanospheres is combined with the smart phone to obtain an actual photographing effect diagram;
FIG. 9 is a diagram based on Ag in example 6 2 The three-channel colorimetric array sensor constructed by the O nanospheres is combined with a smart phone to realize a distinguishing detection diagram of six organophosphorus pesticides.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
The terms "first," "second," "third," and "fourth" and the like in the description and in the claims and drawings are used for distinguishing between different objects and not necessarily for describing a particular sequential or chronological order. Furthermore, the terms "comprise" and "have," as well as any variations thereof, are intended to cover a non-exclusive inclusion. For example, inclusion of a list of steps, processes, methods, etc. is not limited to the listed steps but may alternatively include steps not listed or may alternatively include other steps inherent to such processes, methods, products, or apparatus.
In order that the above-recited objects, features and advantages of the present application will become more readily apparent, a more particular description of the application will be rendered by reference to the appended drawings and appended detailed description.
A method for preparing silver oxide nanospheres, comprising:
step 1: dropwise adding ammonia water into the silver nitrate solution in the stirring process to form a mixed solution;
step 2: dropwise adding sodium hydroxide solution into the mixed solution to obtain a precipitate; wherein the concentration of the ammonia water is 0.05M, the concentration of the silver nitrate solution is 0.05M, the concentration of the sodium hydroxide solution is 2M, and the volume ratio of the ammonia water, the silver nitrate solution and the sodium hydroxide solution is 20:10:1.
step 3: collecting the precipitate, and standing for 12 hours at room temperature in a dark place;
step 4: washing the precipitate after standing, and drying the precipitate at 45 ℃ in a dark place for 12 hours to obtain the silver oxide nanospheres.
The following describes the application in connection with specific examples:
example 1:
5mL of 0.05M aqueous ammonia was added dropwise to 2.5mL of 0.05MAgNO with magnetic stirring 3 Is a kind of medium. After magnetic stirring for 10min, 0.25mL of 2M NaOH was added dropwise to the resulting solution under stirring, giving rise to a large amount of brownish-black precipitate. Then transferred to a 10mL centrifuge tube and allowed to stand at room temperature in the dark for 12h. And centrifugally collecting the precipitate, washing the precipitate with distilled water and absolute ethyl alcohol for multiple times, and drying the precipitate in an oven at 45 ℃ in a dark place for 12 hours to obtain the silver oxide nanospheres with excellent oxidase-like activity particle sizes between 100 and 300 nm. FIG. 1 shows the Ag obtained in example 1 2 As shown in a scanning electron microscope image of the O nanospheres, the Ag prepared by the embodiment of the application can be seen from the image 2 The O nano material is spherical, and the particle size range is 100-300 nm. FIG. 2 is Ag 2 X-ray diffraction pattern of O nanospheres, as shown in the figure, from the figureIt can be seen that Ag prepared in the examples of the present application 2 All diffraction peaks of the O nanospheres are equal to Ag 2 O crystal corresponds (JCPDS, 43-0997).
The application also provides a method for applying the silver oxide nanospheres in organophosphorus pesticide detection, which comprises the following steps:
adding silver oxide nanosphere solution into organophosphorus pesticide to be detected to form pesticide detection solution; the organophosphorus pesticide comprises any one or more of Phoxim (Phoxim), chlorpyrifos (Chlorpyrifos), dimethoate (Dimethoate), triazophos (Triazophos), parathion-methyl (Parath-methyl) and Trichlorphon (Trichlorphon).
Adding a chromogenic substrate into the pesticide detection liquid, and shaking uniformly to obtain a solution to be analyzed; the chromogenic substrate comprises: 3,3', 5' -Tetramethylbenzidine (TMB), o-phenylenediamine (OPD), 2' -azido-bis (3-ethylbenzothiazoline-6-sulfonic Acid) (ABTS).
And carrying out colorimetric detection on the solution to be analyzed to obtain the class or concentration of the corresponding organophosphorus pesticide. In the solution to be analyzed, the concentration of the silver oxide nanosphere solution is 15-50 mug/mL, the concentration of 3,3', 5' -tetramethylbenzidine, o-phenylenediamine and 2,2' -azido-bis (3-ethylbenzothiazoline-6-sulfonic acid) is 2mM, 5mM and 2.5mM respectively, and the concentration of the organophosphorus pesticide is 1-100ng/mL.
The silver oxide nanospheres can catalyze and oxidize TMB, OPD, ABTS three substrates, then blue oxTMB, yellow oxOPD and green oxABTS are generated, a three-way colorimetric array sensor can be formed, according to the different degrees of influence of organophosphorus pesticides on the catalytic activity of the silver oxide nanospheres, a silver oxide solution and an organophosphorus pesticide solution to be detected are mixed and then incubated for a period of time, and then a prepared chromogenic substrate solution (TMB, OPD, ABTS) is added for detection by a colorimetric method.
Furthermore, the solution to be analyzed can be placed on a 96-well plate, and a mobile phone is used for shooting the 96-well plate to obtain a shooting picture; the APP in the mobile phone is utilized to carry out color taking on the photo to obtain RGB values of the corresponding organophosphorus pesticides; and (5) completing the differentiation of the corresponding organophosphorus pesticide types according to the RGB values.
The silver oxide nanospheres prepared by the application do not need to add natural acetylcholinesterase in the process of detecting organophosphorus pesticides, well overcome the defects of unstable natural enzymes, easy inactivation, high price and the like, and simultaneously construct a series of non-selective or semi-selective sensing units by taking three chromogenic substrates as sensing channels, greatly reduce the specific requirements on materials and greatly expand the range of detection objects, so that the array sensor has great advantages in the aspect of complex sample detection.
The following describes the detection process of pesticides in detail with reference to specific examples:
example 2: based on Ag 2 The three-channel array sensor constructed by the O nanospheres is used for high-flux qualitative detection of organophosphorus pesticides.
Ag prepared in example 1 2 The O nanospheres were prepared as 150. Mu.g/mL solutions, 100. Mu.L of the solution was removed with a gun head and added to a centrifuge tube, followed by 100. Mu.L of 100ng/mL organophosphorus pesticide solution, 700. Mu.L of the prepared buffer solution (20 mM NaAc-HAc, pH=4.0) and finally 100. Mu.L of chromogenic substrate TMB at 20 mM. Similarly, ag to be prepared 2 The O nanospheres were prepared as 200. Mu.g/mL solutions, 100. Mu.L were removed with a gun head and added to a centrifuge tube, followed by 100. Mu.L of 100ng/mL organophosphorus pesticide solution, 700. Mu.L of the prepared buffer solution (20 mM NAAc-HAc, pH=4.0) and finally 100. Mu.L of chromogenic substrate OPD at a concentration of 50 mM. Similarly, ag to be prepared 2 The O nanospheres were prepared as 500. Mu.g/mL solutions, 100. Mu.L of the solution was removed with a gun head and added to a centrifuge tube, followed by 100. Mu.L of 100ng/mL organophosphorus pesticide solution, 700. Mu.L of the prepared buffer solution (20 mM NAAc-HAc, pH=4.0) and finally 100. Mu.L of 25mM chromogenic substrate ABTS. After shaking on a shaker, 500. Mu.L of the solution was taken out with a gun head and added to a cuvette, and colorimetric detection was performed using an ultraviolet-visible light absorption spectrometer (UV-2450, japan).
The absorbance of the blank solution detected when the organophosphorus pesticide solution is not added is recorded as A 0 Detecting the absorbance of an organophosphorus pesticide solution upon addition of the solutionThe absorbance was recorded and processed with software. Three array channels are constructed by utilizing three chromogenic substrates, and various organophosphorus pesticides are added, so that the organophosphorus pesticides can be identified efficiently and rapidly through different response signals. In this example, six types of organophosphorus pesticides including Phoxim (Phoxim), chlorpyrifos (Chlorpyrifos), dimethoate (Dimethoate), triazophos (Triazophos), parathion-methyl (Parathion-methyl), and trichlorfon (Trichlorphon) were detected, and six parallel samples were measured for each organophosphorus pesticide, and efficient discrimination of six organophosphorus pesticides was achieved by pattern recognition. Ag based as shown in fig. 3 2 O-constructed three-channel array sensor is used for identifying various organophosphorus pesticides schematically, and is based on Ag shown in FIG. 4 2 And the three-channel colorimetric array sensor constructed by the O realizes a response mode diagram for distinguishing six organophosphorus pesticides under the condition that the final concentration is 10 ng/mL.
Example 3: based on Ag 2 The three-channel array sensor constructed by the O nanospheres is used for quantitatively detecting organophosphorus pesticide dimethoate.
Ag prepared in example 2 2 The O nanosphere solution was removed with a gun head and added to a centrifuge tube, followed by 100. Mu.L of 100-5000ng/mL organophosphorus pesticide solution, 700. Mu.L of the prepared buffer solution (20 mM NaAc-HAc, pH=4.0) and finally 100. Mu.L of chromogenic substrate TMB/OPD/ABTS. After shaking on a shaker, 500. Mu.L of the solution was taken out with a gun head and added to a cuvette, and colorimetric detection was performed using an ultraviolet-visible light absorption spectrometer (UV-2450, japan). This example quantitatively measures and distinguishes Dimethoate at different concentrations. Six parallel samples are measured for each concentration, and efficient quantitative detection and distinction of the dimethoate with different concentrations are realized through pattern recognition. Ag based as shown in fig. 5 2 O is a three-channel colorimetric array sensor response mode diagram constructed by O for different concentrations of dimethoate.
Example 4: based on Ag 2 The three-channel array sensor constructed by the O nanospheres is used for distinguishing and detecting binary mixed organophosphorus pesticides.
Ag prepared in example 2 2 Taking out 100 μl of O nanosphere solution with gun head, adding into ionTo the tube, 100. Mu.L of a mixture of phoxim and trichlorfon in different proportions was added, followed by 700. Mu.L of the prepared buffer (20 mM NaAc-HAc, pH=4.0) and finally 100. Mu.L of chromogenic substrate TMB/OPD/ABTS. After shaking on a shaker, 500. Mu.L of the solution was taken out with a gun head and added to a cuvette, and colorimetric detection was performed using an ultraviolet-visible light absorption spectrometer (UV-2450, japan). This example measured and differentiated phoxim and trichlorfon mixed in different proportions. Six parallel samples are measured for each mixed proportion concentration, and the distinguishing detection of the binary mixed organophosphorus pesticide phoxim and trichlorfon is realized through pattern recognition. Ag based as shown in fig. 6 2 And (3) a response mode diagram of the three-channel colorimetric array sensor constructed by O to binary mixed organophosphorus pesticides phoxim and trichlorfon.
Example 5: based on Ag 2 The three-channel array sensor constructed by the O nanospheres is used for distinguishing and detecting organophosphorus pesticides remained on the epidermis of the melon.
Firstly, melon is washed, and the washing liquid is collected, then 6 organophosphorus pesticide solutions are added into the collected washing liquid sample, and the Ag prepared in example 2 2 The solution of O nanospheres was removed with a gun head and added to a centrifuge tube, followed by 100. Mu.L of a sample solution of the wash solution with the organophosphorus pesticide added at a concentration of 200ng/mL, 700. Mu.L of the prepared buffer solution (20 mM NaAc-HAc, pH=4.0) and finally 100. Mu.L of chromogenic substrate TMB/OPD/ABTS. After shaking on a shaker, 500. Mu.L of the solution was taken out with a gun head and added to a cuvette, and colorimetric detection was performed using an ultraviolet-visible light absorption spectrometer (UV-2450, japan). According to the embodiment, the organophosphorus pesticide residues on the surfaces of the melon samples are measured and distinguished, six parallel samples are measured for each organophosphorus pesticide, and the organophosphorus pesticides in the melon samples are distinguished and detected through pattern recognition. Ag based as shown in fig. 7 2 And (3) a response mode diagram of the three-channel colorimetric array sensor constructed by the O to organophosphorus pesticides in melon cleaning liquid.
Example 6: based on Ag 2 The three-channel array sensor constructed by the O nanospheres is combined with the smart phone to realize detection and differentiation of organophosphorus pesticides.
In order to further simplify the detection flow of the application, the on-site rapid detection is realized by combining the detection flow with a smart phone, and the specific operation is as follows: 200 μl of the liquid to be measured is placed on a 96-well plate, the 96-well plate is placed in a box with airtight, a smart phone is used for shooting at the vertical upper part of the 96-well plate, and the vertical distance between a lens of the smart phone and the 96-well plate is 30cm. The ColorMaxAPP of the smart phone is utilized to obtain respective RGB values, so that the distinction of 6 pesticides at the concentration of 20ng/mL is realized. Ag based as shown in fig. 8 2 And O, combining the constructed three-channel colorimetric array sensor with the smart phone to obtain an actual photographing effect diagram. Ag-based as shown in fig. 9 2 And (3) combining the three-channel colorimetric array sensor constructed by the O with a smart phone to obtain a layer clustering analysis chart of six organophosphorus pesticide detection results.
According to the specific embodiment provided by the application, the application discloses the following beneficial effects:
1. by synthesizing Ag with excellent oxidase-like activity 2 O nanomaterial which can catalyze and oxidize three chromogenic substrates and pass organophosphorus pesticide and Ag under the condition of no participation of natural enzyme 2 The O nano material directly acts, so that the simultaneous detection and the distinction of various organophosphorus pesticides are realized.
2. The method has low detection limit, and can realize the percentage differentiation of 6 organophosphorus pesticides under the condition of the lowest concentration of 10 ng/mL.
3. When one of the organophosphorus pesticides, such as Dimethoate, is quantitatively detected, the organophosphorus pesticide has high sensitivity and wide linear range, the detection limit is 3.1ng/mL, and the linear range is 10-500ng/mL.
4. At a total concentration of 50ng/mL, detection discrimination of binary mixtures, such as phoxim and trichlorfon, dimethoate and methyl parathion, can be achieved.
5. By combining with a smart phone, the on-site rapid detection of binary mixtures, such as dimethoate and methyl parathion, can be realized at a total concentration of 50 ng/mL.
6. The detection method is simple to operate and easy to detect on site.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other. For the method disclosed in the embodiment, since it corresponds to the device disclosed in the embodiment, the description is relatively simple, and the relevant points are referred to the device part description.
The principles and embodiments of the present application have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present application and the core ideas thereof; also, it is within the scope of the present application to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the application.

Claims (9)

1. The preparation method of the silver oxide nanospheres is characterized by comprising the following steps:
dropwise adding ammonia water into the silver nitrate solution in the stirring process to form a mixed solution;
dropwise adding sodium hydroxide solution into the mixed solution to obtain a precipitate;
collecting the precipitate, and standing for 12 hours at room temperature in a dark place;
washing and light-shielding drying the precipitate after standing to obtain the silver oxide nanospheres.
2. The method for preparing silver oxide nanospheres according to claim 1, wherein the concentration of the ammonia water is 0.05M, the concentration of the silver nitrate solution is 0.05M, and the concentration of the sodium hydroxide solution is 2M.
3. The method for preparing silver oxide nanospheres according to claim 2, wherein the volume ratio of the ammonia water, the silver nitrate solution and the sodium hydroxide solution is 20:10:1.
4. the method for preparing silver oxide nanospheres according to claim 3, wherein the precipitate is dried in the dark for 12 hours at 45 ℃.
5. A method for applying silver oxide nanospheres in organophosphorus pesticide detection, which is characterized by comprising the following steps:
adding silver oxide nanosphere solution into organophosphorus pesticide to be detected to form pesticide detection solution;
adding a chromogenic substrate into the pesticide detection liquid, and shaking uniformly to obtain a solution to be analyzed;
and carrying out colorimetric detection on the solution to be analyzed to obtain the class or concentration of the corresponding organophosphorus pesticide.
6. The method for applying silver oxide nanospheres to detection of organophosphorus pesticides of claim 5, wherein the organophosphorus pesticides comprise any one or more of phoxim, chlorpyrifos, dimethoate, triazophos, methylparathion and trichlorfon.
7. The method for applying silver oxide nanospheres to organophosphorus pesticide detection as claimed in claim 5, wherein the chromogenic substrate comprises: 3,3', 5' -tetramethylbenzidine, o-phenylenediamine and 2,2' -azido-bis (3-ethylbenzothiazoline-6-sulfonic acid).
8. The method for applying the silver oxide nanospheres to detection of organophosphorus pesticides according to claim 5, wherein the concentration of the silver oxide nanospheres in the solution to be analyzed is 15-50 μg/mL, the concentration of 3,3', 5' -tetramethylbenzidine, o-phenylenediamine and 2,2' -azido-bis (3-ethylbenzothiazoline-6-sulfonic acid) are 2mM, 5mM and 2.5mM respectively, and the concentration of the organophosphorus pesticides is 1-100ng/mL.
9. The method for applying the silver oxide nanospheres to the detection of organophosphorus pesticides according to claim 5, wherein colorimetric detection of the solution to be analyzed to obtain the class or concentration of the corresponding organophosphorus pesticides comprises the following steps:
placing the solution to be analyzed on a 96-well plate, and shooting the 96-well plate by using a mobile phone to obtain a shooting picture;
the APP in the mobile phone is utilized to carry out color taking on the photo to obtain RGB values of the corresponding organophosphorus pesticides;
and (5) completing the differentiation of the corresponding organophosphorus pesticide types according to the RGB values.
CN202211371649.5A 2022-11-03 2022-11-03 Preparation method of silver oxide nanospheres and application of silver oxide nanospheres in organophosphorus pesticide detection Pending CN116924454A (en)

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