CN116917474A - 多参数发现和优化平台 - Google Patents

多参数发现和优化平台 Download PDF

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Publication number
CN116917474A
CN116917474A CN202180090611.8A CN202180090611A CN116917474A CN 116917474 A CN116917474 A CN 116917474A CN 202180090611 A CN202180090611 A CN 202180090611A CN 116917474 A CN116917474 A CN 116917474A
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China
Prior art keywords
cells
variants
library
variant
polymer
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CN202180090611.8A
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English (en)
Chinese (zh)
Inventor
杰里米·阿格雷斯蒂
安德烈斯·奥尼拉斯瓦加斯
凯文·格雷戈里·霍夫
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Tripbar Biotech
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Tripbar Biotech
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Publication of CN116917474A publication Critical patent/CN116917474A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20—Supervised data analysis
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/50—Mutagenesis
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/10—Design of libraries
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B99/00—Subject matter not provided for in other groups of this subclass

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Molecular Biology (AREA)
  • Medical Informatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Data Mining & Analysis (AREA)
  • Ecology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Artificial Intelligence (AREA)
  • Bioethics (AREA)
  • Databases & Information Systems (AREA)
  • Epidemiology (AREA)
  • Evolutionary Computation (AREA)
  • Public Health (AREA)
  • Software Systems (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Library & Information Science (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180090611.8A 2020-11-13 2021-11-13 多参数发现和优化平台 Pending CN116917474A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063113571P 2020-11-13 2020-11-13
US63/113,571 2020-11-13
PCT/US2021/059275 WO2022104164A1 (en) 2020-11-13 2021-11-13 Multiparametric discovery and optimization platform

Publications (1)

Publication Number Publication Date
CN116917474A true CN116917474A (zh) 2023-10-20

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Family Applications (1)

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CN202180090611.8A Pending CN116917474A (zh) 2020-11-13 2021-11-13 多参数发现和优化平台

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US (3) US11972840B2 (cg-RX-API-DMAC7.html)
EP (1) EP4244353A4 (cg-RX-API-DMAC7.html)
JP (1) JP2023549850A (cg-RX-API-DMAC7.html)
CN (1) CN116917474A (cg-RX-API-DMAC7.html)
GB (1) GB2617285A (cg-RX-API-DMAC7.html)
WO (1) WO2022104164A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119580852A (zh) * 2024-11-19 2025-03-07 中国热带农业科学院环境与植物保护研究所 一种宏基因组数据集的生物地球化学元素循环分析方法及系统

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022104164A1 (en) 2020-11-13 2022-05-19 Triplebar Bio, Inc. Multiparametric discovery and optimization platform
CN116047038B (zh) * 2022-12-21 2025-04-25 南雄市沃太化工有限公司 一种树脂固化条件的实验方法、装置、存储介质及设备
WO2025184561A1 (en) * 2024-03-01 2025-09-04 Deepmind Technologies Limited Optimizing molecule fitness using multiple diverse molecule design techniques

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US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
US8005620B2 (en) * 2003-08-01 2011-08-23 Dna Twopointo Inc. Systems and methods for biopolymer engineering
WO2009102901A1 (en) * 2008-02-12 2009-08-20 Codexis, Inc. Method of generating an optimized, diverse population of variants
WO2010111231A1 (en) * 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US9984201B2 (en) * 2015-01-18 2018-05-29 Youhealth Biotech, Limited Method and system for determining cancer status
PT3289362T (pt) 2015-04-30 2022-06-21 European Molecular Biology Laboratory Deteção e classificação de gotas microfluídicas
CA3007840C (en) 2015-12-07 2020-09-15 Zymergen Inc. Microbial strain improvement by a htp genomic engineering platform
WO2018035602A1 (en) 2016-08-22 2018-03-01 Sci-Bots Inc. Multiplexed droplet actuation and sensing in digital microfluidics
JP7227162B2 (ja) * 2017-06-06 2023-02-21 ザイマージェン インコーポレイテッド 真菌株を改良するためのｈｔｐゲノム操作プラットフォーム
US12374423B2 (en) * 2017-06-27 2025-07-29 The Broad Institute, Inc Systems and methods for MHC class II epitope prediction
US20190345566A1 (en) * 2017-07-12 2019-11-14 The General Hospital Corporation Cancer polygenic risk score
PL3765615T3 (pl) * 2018-03-14 2023-11-13 Arbor Biotechnologies, Inc. Nowe enzymy i układy crispr ukierunkowane na dna
US11028402B2 (en) 2018-04-06 2021-06-08 Wisconsin Alumni Research Foundation Polypeptide and yeast cell compositions and methods of using the same
WO2020148956A1 (ja) * 2019-01-18 2020-07-23 富士フイルム株式会社 細胞生成支援装置、方法、及びプログラム
CN114651064A (zh) 2019-09-13 2022-06-21 芝加哥大学 使用机器学习对蛋白质和其它序列定义的生物分子进行进化数据驱动设计的方法和设备
WO2022104164A1 (en) 2020-11-13 2022-05-19 Triplebar Bio, Inc. Multiparametric discovery and optimization platform

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119580852A (zh) * 2024-11-19 2025-03-07 中国热带农业科学院环境与植物保护研究所 一种宏基因组数据集的生物地球化学元素循环分析方法及系统

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Publication number Publication date
US20230174968A1 (en) 2023-06-08
US20250061962A1 (en) 2025-02-20
GB2617285A (en) 2023-10-04
US11972840B2 (en) 2024-04-30
GB202308819D0 (en) 2023-07-26
WO2022104164A1 (en) 2022-05-19
JP2023549850A (ja) 2023-11-29
US20230154562A1 (en) 2023-05-18
EP4244353A1 (en) 2023-09-20
EP4244353A4 (en) 2024-10-30
US11923045B2 (en) 2024-03-05

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